RU2651483C2 - Способы очистки антител - Google Patents
Способы очистки антител Download PDFInfo
- Publication number
- RU2651483C2 RU2651483C2 RU2015136860A RU2015136860A RU2651483C2 RU 2651483 C2 RU2651483 C2 RU 2651483C2 RU 2015136860 A RU2015136860 A RU 2015136860A RU 2015136860 A RU2015136860 A RU 2015136860A RU 2651483 C2 RU2651483 C2 RU 2651483C2
- Authority
- RU
- Russia
- Prior art keywords
- protein
- acid
- sodium
- buffer
- contaminant
- Prior art date
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- 238000000034 method Methods 0.000 title claims abstract description 74
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 174
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 172
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims abstract description 120
- 239000011534 wash buffer Substances 0.000 claims abstract description 58
- 239000000356 contaminant Substances 0.000 claims abstract description 56
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- -1 aliphatic carboxylate Chemical class 0.000 claims abstract description 39
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims abstract description 34
- BYKRNSHANADUFY-UHFFFAOYSA-M sodium octanoate Chemical compound [Na+].CCCCCCCC([O-])=O BYKRNSHANADUFY-UHFFFAOYSA-M 0.000 claims abstract description 33
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- 231100000617 superantigen Toxicity 0.000 claims abstract description 28
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- FIWQZURFGYXCEO-UHFFFAOYSA-M sodium;decanoate Chemical compound [Na+].CCCCCCCCCC([O-])=O FIWQZURFGYXCEO-UHFFFAOYSA-M 0.000 claims abstract description 16
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- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 18
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- 235000017281 sodium acetate Nutrition 0.000 claims description 18
- WWZKQHOCKIZLMA-UHFFFAOYSA-M octanoate Chemical compound CCCCCCCC([O-])=O WWZKQHOCKIZLMA-UHFFFAOYSA-M 0.000 claims description 17
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- IHPYMWDTONKSCO-UHFFFAOYSA-N 2,2'-piperazine-1,4-diylbisethanesulfonic acid Chemical compound OS(=O)(=O)CCN1CCN(CCS(O)(=O)=O)CC1 IHPYMWDTONKSCO-UHFFFAOYSA-N 0.000 claims description 4
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 claims description 4
- DVLFYONBTKHTER-UHFFFAOYSA-N 3-(N-morpholino)propanesulfonic acid Chemical compound OS(=O)(=O)CCCN1CCOCC1 DVLFYONBTKHTER-UHFFFAOYSA-N 0.000 claims description 4
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- JOCBASBOOFNAJA-UHFFFAOYSA-N N-tris(hydroxymethyl)methyl-2-aminoethanesulfonic acid Chemical compound OCC(CO)(CO)NCCS(O)(=O)=O JOCBASBOOFNAJA-UHFFFAOYSA-N 0.000 claims description 4
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- YMAWOPBAYDPSLA-UHFFFAOYSA-N glycylglycine Chemical compound [NH3+]CC(=O)NCC([O-])=O YMAWOPBAYDPSLA-UHFFFAOYSA-N 0.000 claims description 4
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- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 2
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 claims description 2
- HRGUSFBJBOKSML-UHFFFAOYSA-N 3',5'-di-O-methyltricetin Chemical compound COC1=C(O)C(OC)=CC(C=2OC3=CC(O)=CC(O)=C3C(=O)C=2)=C1 HRGUSFBJBOKSML-UHFFFAOYSA-N 0.000 claims description 2
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 claims description 2
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
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- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 2
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- KDYFGRWQOYBRFD-UHFFFAOYSA-N Succinic acid Natural products OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 2
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- 229910052783 alkali metal Inorganic materials 0.000 claims description 2
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| EP3680000B1 (en) | 2013-03-15 | 2024-03-13 | GlaxoSmithKline Intellectual Property Development Limited | Methods for purifying antibodies |
| CA2910065C (en) | 2013-05-15 | 2023-09-19 | Medimmune Limited | Purification of recombinantly produced polypeptides |
| EP3842108A1 (en) | 2014-04-30 | 2021-06-30 | Novo Nordisk A/S | Methods for the purification of proteins using caprylic acid |
| JP6663360B2 (ja) | 2015-01-26 | 2020-03-11 | 株式会社カネカ | 変異型免疫グロブリンκ鎖可変領域結合性ペプチド |
| WO2016121701A1 (ja) | 2015-01-26 | 2016-08-04 | 株式会社カネカ | 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス |
| WO2017195638A1 (ja) * | 2016-05-09 | 2017-11-16 | 株式会社カネカ | 抗体またはκ鎖可変領域含有抗体断片の精製方法 |
| EP4389757A3 (en) * | 2016-09-07 | 2025-01-01 | GlaxoSmithKline Intellectual Property Development Limited | Methods for purifying antibodies |
| CN106939045B (zh) * | 2017-05-01 | 2020-03-31 | 深圳市国创纳米抗体技术有限公司 | 一种单克隆抗体细胞培养液澄清的方法 |
| CN108085358A (zh) * | 2018-02-08 | 2018-05-29 | 保定冀中药业有限公司 | 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法 |
| TW202003555A (zh) | 2018-03-07 | 2020-01-16 | 英商葛蘭素史克智慧財產發展有限公司 | 用於純化重組多肽之方法 |
| TW202000891A (zh) | 2018-03-07 | 2020-01-01 | 英商葛蘭素史克智慧財產發展有限公司 | 純化抗體之方法 |
| JP2023539163A (ja) * | 2020-08-18 | 2023-09-13 | ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー． | 宿主細胞ｄｎａの金属アフィニティー抽出 |
| CN114133446A (zh) * | 2021-12-31 | 2022-03-04 | 方坦思(上海)生物医药有限公司 | 单克隆抗体的纯化方法 |
| CN116179582A (zh) * | 2022-09-27 | 2023-05-30 | 南京医事达生物科技有限公司 | 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用 |
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| US5429746A (en) * | 1994-02-22 | 1995-07-04 | Smith Kline Beecham Corporation | Antibody purification |
| US6955917B2 (en) | 1997-06-20 | 2005-10-18 | Bayer Healthcare Llc | Chromatographic method for high yield purification and viral inactivation of antibodies |
| IL127127A0 (en) | 1998-11-18 | 1999-09-22 | Peptor Ltd | Small functional units of antibody heavy chain variable regions |
| DK2272378T3 (da) * | 2001-06-01 | 2014-10-06 | Upfront Chromatography As | Fraktionering af proteinholdige blandinger |
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| WO2003102208A2 (en) | 2002-05-31 | 2003-12-11 | Indian Council Of Agricultural Research | Rapid detection of bt-cry toxins |
| PT1718675E (pt) * | 2004-02-27 | 2013-05-21 | Octapharma Ag | Método de proporcionar uma preparação purificada de anticorpos livre de vírus |
| JP2008500972A (ja) * | 2004-05-14 | 2008-01-17 | キリンファーマ株式会社 | 免疫グロブリンの精製方法 |
| US8263750B2 (en) * | 2006-03-16 | 2012-09-11 | Amgen Inc. | Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step |
| AU2007292253A1 (en) | 2006-09-08 | 2008-03-13 | Wyeth | Arginine wash in protein purification using affinity chromatography |
| US20080167450A1 (en) * | 2007-01-05 | 2008-07-10 | Hai Pan | Methods of purifying proteins |
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| IL244149B2 (en) | 2013-09-13 | 2025-09-01 | Genentech Inc | Methods and compositions containing purified recombinant polypeptides |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2012135415A1 (en) * | 2011-03-29 | 2012-10-04 | Glaxosmithkline Llc | Buffer system for protein purification |
Non-Patent Citations (2)
| Title |
|---|
| WO 2012135415 A1, 04.10.2012, формула. Brodsky Y. et al. Caprylic acid precipitation method for impurity reduction: an alternative to conventional chromatography for monoclonal antibody purification //Biotechnology and bioengineering. - 2012. - Vol. 109. - n. 10. - P. 2589-2598. * |
| формула. Brodsky Y. et al. Caprylic acid precipitation method for impurity reduction: an alternative to conventional chromatography for monoclonal antibody purification //Biotechnology and bioengineering. - 2012. - Vol. 109. - n. 10. - P. 2589-2598. * |
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