AU2014229280B2 - Methods for purifying antibodies - Google Patents

Methods for purifying antibodies Download PDF

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Publication number
AU2014229280B2
AU2014229280B2 AU2014229280A AU2014229280A AU2014229280B2 AU 2014229280 B2 AU2014229280 B2 AU 2014229280B2 AU 2014229280 A AU2014229280 A AU 2014229280A AU 2014229280 A AU2014229280 A AU 2014229280A AU 2014229280 B2 AU2014229280 B2 AU 2014229280B2
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AU
Australia
Prior art keywords
protein
acid
ppm
sodium
tris
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2014229280A
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English (en)
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AU2014229280A1 (en
Inventor
Kent E. Goklen
Eric J. Suda
Antonio Raul Ubiera
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
GlaxoSmithKline Intellectual Property No 2 Ltd
Original Assignee
GlaxoSmithKline Intellectual Property No 2 Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Application filed by GlaxoSmithKline Intellectual Property No 2 Ltd filed Critical GlaxoSmithKline Intellectual Property No 2 Ltd
Publication of AU2014229280A1 publication Critical patent/AU2014229280A1/en
Application granted granted Critical
Publication of AU2014229280B2 publication Critical patent/AU2014229280B2/en
Ceased legal-status Critical Current
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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2014229280A 2013-03-15 2014-03-13 Methods for purifying antibodies Ceased AU2014229280B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361787309P 2013-03-15 2013-03-15
US61/787,309 2013-03-15
PCT/IB2014/059755 WO2014141150A1 (en) 2013-03-15 2014-03-13 Methods for purifying antibodies

Publications (2)

Publication Number Publication Date
AU2014229280A1 AU2014229280A1 (en) 2015-10-01
AU2014229280B2 true AU2014229280B2 (en) 2017-01-05

Family

ID=50391244

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2014229280A Ceased AU2014229280B2 (en) 2013-03-15 2014-03-13 Methods for purifying antibodies

Country Status (15)

Country Link
US (3) US10676503B2 (enExample)
EP (3) EP3680000B1 (enExample)
JP (2) JP6599773B2 (enExample)
KR (1) KR102114021B1 (enExample)
CN (1) CN105188872B (enExample)
AU (1) AU2014229280B2 (enExample)
BR (1) BR112015022321A2 (enExample)
CA (1) CA2902287A1 (enExample)
DK (2) DK3680000T3 (enExample)
ES (2) ES2978309T3 (enExample)
IL (1) IL240753B (enExample)
RU (1) RU2651483C2 (enExample)
SG (1) SG11201506494XA (enExample)
TW (1) TWI625335B (enExample)
WO (1) WO2014141150A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3680000B1 (en) * 2013-03-15 2024-03-13 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
WO2014186350A1 (en) 2013-05-15 2014-11-20 Medimmune Limited Purification of recombinantly produced polypeptides
EP3137186B1 (en) 2014-04-30 2021-01-27 Novo Nordisk A/S Methods for the purification of proteins using caprylic acid
JPWO2016121701A1 (ja) 2015-01-26 2017-11-09 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
EP3252158A4 (en) 2015-01-26 2018-07-18 Kaneka Corporation Mutant immunoglobulin kappa chain variable region-binding peptide
WO2017195638A1 (ja) * 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
EP3510042B1 (en) * 2016-09-07 2024-05-22 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
CN106939045B (zh) * 2017-05-01 2020-03-31 深圳市国创纳米抗体技术有限公司 一种单克隆抗体细胞培养液澄清的方法
CN108085358A (zh) * 2018-02-08 2018-05-29 保定冀中药业有限公司 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法
TW202003555A (zh) 2018-03-07 2020-01-16 英商葛蘭素史克智慧財產發展有限公司 用於純化重組多肽之方法
TW202000891A (zh) * 2018-03-07 2020-01-01 英商葛蘭素史克智慧財產發展有限公司 純化抗體之方法
EP4200048A1 (en) * 2020-08-18 2023-06-28 Sartorius BIA Separations d.o.o. Metal-affinity extraction of host cell dna
CN114133446A (zh) * 2021-12-31 2022-03-04 方坦思(上海)生物医药有限公司 单克隆抗体的纯化方法
CN116179582A (zh) * 2022-09-27 2023-05-30 南京医事达生物科技有限公司 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用

Citations (2)

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WO2005082937A2 (en) * 2004-02-27 2005-09-09 Octapharma Ag A method of providing a purified, virus safe antibody preparation
WO2007109163A2 (en) * 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using

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JPS57106673A (en) 1980-12-24 1982-07-02 Chugai Pharmaceut Co Ltd Dibenzo(b,f)(1,4)oxazepin derivative
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US6955917B2 (en) 1997-06-20 2005-10-18 Bayer Healthcare Llc Chromatographic method for high yield purification and viral inactivation of antibodies
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
ATE290323T1 (de) 2001-06-01 2005-03-15 Upfront Chromatography As Fraktionierung von proteinhaltigen mischungen
ES2337986T3 (es) 2001-08-10 2010-05-03 Aberdeen University Dominios de union de antigenos de peces.
KR20050026396A (ko) 2002-05-31 2005-03-15 인디언 카운실 오브 애그리컬쳐럴 리서치 Ｂｔ-ｃｒｙ 독소의 고속 검출법
US20050272917A1 (en) * 2004-05-14 2005-12-08 Hematech, Llc Methods for immunoglobulin purification
WO2008031020A2 (en) 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography
WO2008085988A1 (en) * 2007-01-05 2008-07-17 Amgen Inc. Methods of purifying proteins
US9624261B2 (en) * 2011-03-29 2017-04-18 Glaxosmithkline Llc Buffer system for protein purification
EP3680000B1 (en) 2013-03-15 2024-03-13 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
WO2014186350A1 (en) 2013-05-15 2014-11-20 Medimmune Limited Purification of recombinantly produced polypeptides
NZ756750A (en) 2013-09-13 2022-05-27 Genentech Inc Methods and compositions comprising purified recombinant polypeptides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005082937A2 (en) * 2004-02-27 2005-09-09 Octapharma Ag A method of providing a purified, virus safe antibody preparation
WO2007109163A2 (en) * 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
BRODSKY YAN et al:'Caprylic acid precipitation method for impurity reduction: an alternative to conventional chromatography for monoclonal antibody purification', BIOTECH AND BIOENG OCT 2012,vol. 109, no. 10, October 2012, pp.2589-2598. *
NILSON B H K et al: "Purification of antibodies using protein L-binding framework structures in the light chain variable domain", JOURNAL OF IMMUNOLOGICAL METHODS, ELSEVIER SCIENCE PUBLISHERS B.V.,AMSTERDAM, NL, vol. 164, no. 1, 26/08/1993. *
TONG HONG-FEI et al: 'Caprylate as the albumin-selective modifier to improve IgG purification with hydrophobic charge-induction chromatography.", JOURNAL OF CHROMATOGRAPHY. A 12 APR 2013, vol. 1285, 15 February 2013, pages 88-96. *

Also Published As

Publication number Publication date
CN105188872B (zh) 2019-01-15
EP2969098B1 (en) 2019-10-30
AU2014229280A1 (en) 2015-10-01
US20250353878A1 (en) 2025-11-20
US20160024146A1 (en) 2016-01-28
RU2015136860A (ru) 2017-04-28
KR102114021B1 (ko) 2020-05-22
JP2020019795A (ja) 2020-02-06
EP4371996A2 (en) 2024-05-22
WO2014141150A1 (en) 2014-09-18
ES2765206T3 (es) 2020-06-08
CA2902287A1 (en) 2014-09-18
US12358945B2 (en) 2025-07-15
BR112015022321A2 (pt) 2017-07-18
TWI625335B (zh) 2018-06-01
EP3680000A1 (en) 2020-07-15
DK3680000T3 (da) 2024-04-02
KR20150131235A (ko) 2015-11-24
SG11201506494XA (en) 2015-09-29
TW201522365A (zh) 2015-06-16
JP7002511B2 (ja) 2022-02-21
IL240753B (en) 2021-01-31
EP4371996A3 (en) 2024-08-14
IL240753A0 (en) 2015-10-29
DK2969098T3 (da) 2020-01-27
RU2651483C2 (ru) 2018-04-19
JP6599773B2 (ja) 2019-10-30
US10676503B2 (en) 2020-06-09
EP3680000B1 (en) 2024-03-13
CN105188872A (zh) 2015-12-23
ES2978309T3 (es) 2024-09-10
EP2969098A1 (en) 2016-01-20
JP2016519052A (ja) 2016-06-30
US20200277330A1 (en) 2020-09-03

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FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired