TWI625335B - 純化抗體的方法 - Google Patents

純化抗體的方法 Download PDF

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Publication number
TWI625335B
TWI625335B TW103108898A TW103108898A TWI625335B TW I625335 B TWI625335 B TW I625335B TW 103108898 A TW103108898 A TW 103108898A TW 103108898 A TW103108898 A TW 103108898A TW I625335 B TWI625335 B TW I625335B
Authority
TW
Taiwan
Prior art keywords
protein
acid
item
buffer
sodium
Prior art date
Application number
TW103108898A
Other languages
English (en)
Chinese (zh)
Other versions
TW201522365A (zh
Inventor
Kent E. Goklen
肯特 蓋肯
Eric J. Suda
艾瑞克 蘇德
Antonio Raul Ubiera
安東尼奧 貝宜勒
Original Assignee
Glaxosmithkline Intellectual Property (No. 2) Limited
葛蘭素史克智慧財產（第二）有限公司
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=50391244&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=TWI625335(B) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Glaxosmithkline Intellectual Property (No. 2) Limited, 葛蘭素史克智慧財產（第二）有限公司 filed Critical Glaxosmithkline Intellectual Property (No. 2) Limited
Publication of TW201522365A publication Critical patent/TW201522365A/zh
Application granted granted Critical
Publication of TWI625335B publication Critical patent/TWI625335B/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
TW103108898A 2013-03-15 2014-03-13 純化抗體的方法 TWI625335B (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201361787309P 2013-03-15 2013-03-15
US61/787,309 2013-03-15

Publications (2)

Publication Number Publication Date
TW201522365A TW201522365A (zh) 2015-06-16
TWI625335B true TWI625335B (zh) 2018-06-01

Family

ID=50391244

Family Applications (1)

Application Number Title Priority Date Filing Date
TW103108898A TWI625335B (zh) 2013-03-15 2014-03-13 純化抗體的方法

Country Status (15)

Country Link
US (3) US10676503B2 (enExample)
EP (3) EP3680000B1 (enExample)
JP (2) JP6599773B2 (enExample)
KR (1) KR102114021B1 (enExample)
CN (1) CN105188872B (enExample)
AU (1) AU2014229280B2 (enExample)
BR (1) BR112015022321A2 (enExample)
CA (1) CA2902287A1 (enExample)
DK (2) DK3680000T3 (enExample)
ES (2) ES2978309T3 (enExample)
IL (1) IL240753B (enExample)
RU (1) RU2651483C2 (enExample)
SG (1) SG11201506494XA (enExample)
TW (1) TWI625335B (enExample)
WO (1) WO2014141150A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3680000B1 (en) 2013-03-15 2024-03-13 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
CA2910065C (en) 2013-05-15 2023-09-19 Medimmune Limited Purification of recombinantly produced polypeptides
EP3842108A1 (en) 2014-04-30 2021-06-30 Novo Nordisk A/S Methods for the purification of proteins using caprylic acid
JP6663360B2 (ja) 2015-01-26 2020-03-11 株式会社カネカ 変異型免疫グロブリンκ鎖可変領域結合性ペプチド
WO2016121701A1 (ja) 2015-01-26 2016-08-04 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
WO2017195638A1 (ja) * 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
EP4389757A3 (en) * 2016-09-07 2025-01-01 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
CN106939045B (zh) * 2017-05-01 2020-03-31 深圳市国创纳米抗体技术有限公司 一种单克隆抗体细胞培养液澄清的方法
CN108085358A (zh) * 2018-02-08 2018-05-29 保定冀中药业有限公司 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法
TW202003555A (zh) 2018-03-07 2020-01-16 英商葛蘭素史克智慧財產發展有限公司 用於純化重組多肽之方法
TW202000891A (zh) 2018-03-07 2020-01-01 英商葛蘭素史克智慧財產發展有限公司 純化抗體之方法
JP2023539163A (ja) * 2020-08-18 2023-09-13 ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー． 宿主細胞ｄｎａの金属アフィニティー抽出
CN114133446A (zh) * 2021-12-31 2022-03-04 方坦思(上海)生物医药有限公司 单克隆抗体的纯化方法
CN116179582A (zh) * 2022-09-27 2023-05-30 南京医事达生物科技有限公司 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1922207A (zh) * 2004-02-27 2007-02-28 奥克特珐玛股份有限公司 提供纯化的、病毒安全的抗体制剂的方法
WO2007109163A2 (en) * 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using

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JPS57106673A (en) 1980-12-24 1982-07-02 Chugai Pharmaceut Co Ltd Dibenzo(b,f)(1,4)oxazepin derivative
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US6955917B2 (en) 1997-06-20 2005-10-18 Bayer Healthcare Llc Chromatographic method for high yield purification and viral inactivation of antibodies
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
DK2272378T3 (da) * 2001-06-01 2014-10-06 Upfront Chromatography As Fraktionering af proteinholdige blandinger
DK1419179T3 (da) 2001-08-10 2010-06-21 Univ Aberdeen Antigenbindingsdomæner fra fisk
WO2003102208A2 (en) 2002-05-31 2003-12-11 Indian Council Of Agricultural Research Rapid detection of bt-cry toxins
JP2008500972A (ja) * 2004-05-14 2008-01-17 キリンファーマ株式会社 免疫グロブリンの精製方法
AU2007292253A1 (en) 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography
US20080167450A1 (en) * 2007-01-05 2008-07-10 Hai Pan Methods of purifying proteins
WO2012135415A1 (en) 2011-03-29 2012-10-04 Glaxosmithkline Llc Buffer system for protein purification
EP3680000B1 (en) 2013-03-15 2024-03-13 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
CA2910065C (en) * 2013-05-15 2023-09-19 Medimmune Limited Purification of recombinantly produced polypeptides
IL244149B2 (en) 2013-09-13 2025-09-01 Genentech Inc Methods and compositions containing purified recombinant polypeptides

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1922207A (zh) * 2004-02-27 2007-02-28 奥克特珐玛股份有限公司 提供纯化的、病毒安全的抗体制剂的方法
WO2007109163A2 (en) * 2006-03-16 2007-09-27 Amgen Inc Wash buffer and method of using

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Brodsky, Yan, et al. "Caprylic acid precipitation method for impurity reduction: an alternative to conventional chromatography for monoclonal antibody purification." Biotechnology and bioengineering109.10 (2012): 2589-2598. *

Also Published As

Publication number Publication date
WO2014141150A1 (en) 2014-09-18
CN105188872A (zh) 2015-12-23
RU2015136860A (ru) 2017-04-28
EP2969098A1 (en) 2016-01-20
IL240753B (en) 2021-01-31
EP4371996A3 (en) 2024-08-14
IL240753A0 (en) 2015-10-29
TW201522365A (zh) 2015-06-16
JP2020019795A (ja) 2020-02-06
US10676503B2 (en) 2020-06-09
EP3680000B1 (en) 2024-03-13
JP7002511B2 (ja) 2022-02-21
CA2902287A1 (en) 2014-09-18
ES2978309T3 (es) 2024-09-10
AU2014229280A1 (en) 2015-10-01
JP6599773B2 (ja) 2019-10-30
US20200277330A1 (en) 2020-09-03
CN105188872B (zh) 2019-01-15
ES2765206T3 (es) 2020-06-08
RU2651483C2 (ru) 2018-04-19
SG11201506494XA (en) 2015-09-29
KR102114021B1 (ko) 2020-05-22
EP2969098B1 (en) 2019-10-30
DK2969098T3 (da) 2020-01-27
EP3680000A1 (en) 2020-07-15
US20160024146A1 (en) 2016-01-28
US12358945B2 (en) 2025-07-15
AU2014229280B2 (en) 2017-01-05
KR20150131235A (ko) 2015-11-24
BR112015022321A2 (pt) 2017-07-18
US20250353878A1 (en) 2025-11-20
EP4371996A2 (en) 2024-05-22
JP2016519052A (ja) 2016-06-30
DK3680000T3 (da) 2024-04-02

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