CN105188872B - 用于纯化抗体的方法 - Google Patents

用于纯化抗体的方法 Download PDF

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Publication number
CN105188872B
CN105188872B CN201480016016.XA CN201480016016A CN105188872B CN 105188872 B CN105188872 B CN 105188872B CN 201480016016 A CN201480016016 A CN 201480016016A CN 105188872 B CN105188872 B CN 105188872B
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China
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albumen
acid
ppm
sodium
washing buffer
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CN201480016016.XA
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English (en)
Chinese (zh)
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CN105188872A (zh
Inventor
K.E.戈克伦
E.J.苏达
A.R.尤比拉
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GlaxoSmithKline Intellectual Property No 2 Ltd
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GlaxoSmithKline Intellectual Property No 2 Ltd
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN201480016016.XA 2013-03-15 2014-03-13 用于纯化抗体的方法 Active CN105188872B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201361787309P 2013-03-15 2013-03-15
US61/787309 2013-03-15
PCT/IB2014/059755 WO2014141150A1 (en) 2013-03-15 2014-03-13 Methods for purifying antibodies

Publications (2)

Publication Number Publication Date
CN105188872A CN105188872A (zh) 2015-12-23
CN105188872B true CN105188872B (zh) 2019-01-15

Family

ID=50391244

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CN201480016016.XA Active CN105188872B (zh) 2013-03-15 2014-03-13 用于纯化抗体的方法

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US (3) US10676503B2 (enExample)
EP (3) EP3680000B1 (enExample)
JP (2) JP6599773B2 (enExample)
KR (1) KR102114021B1 (enExample)
CN (1) CN105188872B (enExample)
AU (1) AU2014229280B2 (enExample)
BR (1) BR112015022321A2 (enExample)
CA (1) CA2902287A1 (enExample)
DK (2) DK3680000T3 (enExample)
ES (2) ES2978309T3 (enExample)
IL (1) IL240753B (enExample)
RU (1) RU2651483C2 (enExample)
SG (1) SG11201506494XA (enExample)
TW (1) TWI625335B (enExample)
WO (1) WO2014141150A1 (enExample)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3680000B1 (en) 2013-03-15 2024-03-13 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
CA2910065C (en) 2013-05-15 2023-09-19 Medimmune Limited Purification of recombinantly produced polypeptides
EP3842108A1 (en) 2014-04-30 2021-06-30 Novo Nordisk A/S Methods for the purification of proteins using caprylic acid
JP6663360B2 (ja) 2015-01-26 2020-03-11 株式会社カネカ 変異型免疫グロブリンκ鎖可変領域結合性ペプチド
WO2016121701A1 (ja) 2015-01-26 2016-08-04 株式会社カネカ 免疫グロブリンκ鎖可変領域含有タンパク質精製用アフィニティー分離マトリックス
WO2017195638A1 (ja) * 2016-05-09 2017-11-16 株式会社カネカ 抗体またはκ鎖可変領域含有抗体断片の精製方法
EP4389757A3 (en) * 2016-09-07 2025-01-01 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
CN106939045B (zh) * 2017-05-01 2020-03-31 深圳市国创纳米抗体技术有限公司 一种单克隆抗体细胞培养液澄清的方法
CN108085358A (zh) * 2018-02-08 2018-05-29 保定冀中药业有限公司 一种犬细小病毒单克隆抗体IgG2b-Fab片段的制备方法
TW202003555A (zh) 2018-03-07 2020-01-16 英商葛蘭素史克智慧財產發展有限公司 用於純化重組多肽之方法
TW202000891A (zh) 2018-03-07 2020-01-01 英商葛蘭素史克智慧財產發展有限公司 純化抗體之方法
JP2023539163A (ja) * 2020-08-18 2023-09-13 ザルトリウス ビーアイエー セパレーションズ ディー．オー．オー． 宿主細胞ｄｎａの金属アフィニティー抽出
CN114133446A (zh) * 2021-12-31 2022-03-04 方坦思(上海)生物医药有限公司 单克隆抗体的纯化方法
CN116179582A (zh) * 2022-09-27 2023-05-30 南京医事达生物科技有限公司 一种通用型基因重组蛋白质a亲和纯化填料制备方法和应用

Citations (1)

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WO2012135415A1 (en) * 2011-03-29 2012-10-04 Glaxosmithkline Llc Buffer system for protein purification

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JPS57106673A (en) 1980-12-24 1982-07-02 Chugai Pharmaceut Co Ltd Dibenzo(b,f)(1,4)oxazepin derivative
GB8607679D0 (en) 1986-03-27 1986-04-30 Winter G P Recombinant dna product
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
US6955917B2 (en) 1997-06-20 2005-10-18 Bayer Healthcare Llc Chromatographic method for high yield purification and viral inactivation of antibodies
IL127127A0 (en) 1998-11-18 1999-09-22 Peptor Ltd Small functional units of antibody heavy chain variable regions
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WO2003102208A2 (en) 2002-05-31 2003-12-11 Indian Council Of Agricultural Research Rapid detection of bt-cry toxins
PT1718675E (pt) * 2004-02-27 2013-05-21 Octapharma Ag Método de proporcionar uma preparação purificada de anticorpos livre de vírus
JP2008500972A (ja) * 2004-05-14 2008-01-17 キリンファーマ株式会社 免疫グロブリンの精製方法
US8263750B2 (en) * 2006-03-16 2012-09-11 Amgen Inc. Method for purifying a protein using protein-A affinity chromatography using an intermediate wash step
AU2007292253A1 (en) 2006-09-08 2008-03-13 Wyeth Arginine wash in protein purification using affinity chromatography
US20080167450A1 (en) * 2007-01-05 2008-07-10 Hai Pan Methods of purifying proteins
EP3680000B1 (en) 2013-03-15 2024-03-13 GlaxoSmithKline Intellectual Property Development Limited Methods for purifying antibodies
CA2910065C (en) * 2013-05-15 2023-09-19 Medimmune Limited Purification of recombinantly produced polypeptides
IL244149B2 (en) 2013-09-13 2025-09-01 Genentech Inc Methods and compositions containing purified recombinant polypeptides

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012135415A1 (en) * 2011-03-29 2012-10-04 Glaxosmithkline Llc Buffer system for protein purification

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Evaluation of a novel agarose-based synthetic ligand adsorbent for the recovery of antibodies from ovine serum;Sunil Chhatre et al.;《Journal of Chromatography B》;20071119;第860卷;摘要，第216页第1段，第213页第3.2节 *
Evaluation of the 96-well format for screening of chromatographic buffer conditions;Elin Monié;《Master’s degree project，Molecular Biotechnology Program，Uppsala University School of Engineering》;20061031;4.1.3.1.2节、4.1.1.1节、第3.1、3.3节，第53页，第55页 *
Optimised affinity purification of polyclonal antibodies from hyper immunised ovine serum using a synthetic Protein A adsorbent,MAbsorbent A2P;Anthony R. Newcombe et al.;《Journal of Chromatography B》;20041111;第814卷;第210第2.1节，第214页第3.3节，附图8 *

Also Published As

Publication number Publication date
WO2014141150A1 (en) 2014-09-18
CN105188872A (zh) 2015-12-23
RU2015136860A (ru) 2017-04-28
EP2969098A1 (en) 2016-01-20
IL240753B (en) 2021-01-31
EP4371996A3 (en) 2024-08-14
IL240753A0 (en) 2015-10-29
TW201522365A (zh) 2015-06-16
JP2020019795A (ja) 2020-02-06
US10676503B2 (en) 2020-06-09
EP3680000B1 (en) 2024-03-13
JP7002511B2 (ja) 2022-02-21
CA2902287A1 (en) 2014-09-18
ES2978309T3 (es) 2024-09-10
AU2014229280A1 (en) 2015-10-01
JP6599773B2 (ja) 2019-10-30
US20200277330A1 (en) 2020-09-03
ES2765206T3 (es) 2020-06-08
RU2651483C2 (ru) 2018-04-19
SG11201506494XA (en) 2015-09-29
KR102114021B1 (ko) 2020-05-22
EP2969098B1 (en) 2019-10-30
TWI625335B (zh) 2018-06-01
DK2969098T3 (da) 2020-01-27
EP3680000A1 (en) 2020-07-15
US20160024146A1 (en) 2016-01-28
US12358945B2 (en) 2025-07-15
AU2014229280B2 (en) 2017-01-05
KR20150131235A (ko) 2015-11-24
BR112015022321A2 (pt) 2017-07-18
US20250353878A1 (en) 2025-11-20
EP4371996A2 (en) 2024-05-22
JP2016519052A (ja) 2016-06-30
DK3680000T3 (da) 2024-04-02

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Address after: Steve Niki

Patentee after: GLAXOSMITHKLINE INTELLECTUAL PROPERTY (NO.2) Ltd.

Country or region after: Britain

Address before: The Middlesex County

Patentee before: GLAXOSMITHKLINE INTELLECTUAL PROPERTY (NO.2) Ltd.

Country or region before: Britain