RU2197524C2 - Термостабильная днк-полимераза, способ ее получения, фрагмент выделенной днк, способ амплификации днк, способ введения метки в днк и способ обратной транскрипции - Google Patents
Термостабильная днк-полимераза, способ ее получения, фрагмент выделенной днк, способ амплификации днк, способ введения метки в днк и способ обратной транскрипции Download PDFInfo
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- RU2197524C2 RU2197524C2 RU99109121/13A RU99109121A RU2197524C2 RU 2197524 C2 RU2197524 C2 RU 2197524C2 RU 99109121/13 A RU99109121/13 A RU 99109121/13A RU 99109121 A RU99109121 A RU 99109121A RU 2197524 C2 RU2197524 C2 RU 2197524C2
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- dna
- polymerase
- activity
- dna polymerase
- reverse transcriptase
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
- C12N9/1241—Nucleotidyltransferases (2.7.7)
- C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
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- Microbiology (AREA)
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- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Saccharide Compounds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96115873.0 | 1996-10-03 | ||
| EP96115873A EP0834569A1 (en) | 1996-10-03 | 1996-10-03 | Thermostable DNA polymerase from carboxydothermus hydrogenoformans |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU99109121A RU99109121A (ru) | 2001-05-20 |
| RU2197524C2 true RU2197524C2 (ru) | 2003-01-27 |
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| RU99109121/13A RU2197524C2 (ru) | 1996-10-03 | 1997-10-01 | Термостабильная днк-полимераза, способ ее получения, фрагмент выделенной днк, способ амплификации днк, способ введения метки в днк и способ обратной транскрипции |
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| Country | Link |
|---|---|
| US (1) | US6468775B1 (enExample) |
| EP (2) | EP0834569A1 (enExample) |
| JP (1) | JP4005637B2 (enExample) |
| AT (1) | ATE357527T1 (enExample) |
| AU (1) | AU717009B2 (enExample) |
| CA (1) | CA2268014C (enExample) |
| DE (1) | DE69737502T2 (enExample) |
| IL (1) | IL129085A (enExample) |
| NO (1) | NO323846B1 (enExample) |
| NZ (1) | NZ334652A (enExample) |
| RU (1) | RU2197524C2 (enExample) |
| WO (1) | WO1998014589A1 (enExample) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733145A (zh) * | 2020-07-01 | 2020-10-02 | 济南国科医工科技发展有限公司 | 重组酶的纯化方法 |
| RU2809366C1 (ru) * | 2022-12-28 | 2023-12-11 | Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) | Способ получения большого фрагмента Bst-полимеразы (варианты) |
Families Citing this family (28)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0921196A1 (en) * | 1997-12-02 | 1999-06-09 | Roche Diagnostics GmbH | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
| EP0922765B1 (en) * | 1997-12-02 | 2005-11-23 | Roche Diagnostics GmbH | Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction |
| US20040009486A1 (en) * | 1999-10-29 | 2004-01-15 | Sorge Joseph A. | Compositions and methods utilizing DNA polymerases |
| US20030228616A1 (en) * | 1999-10-29 | 2003-12-11 | Stratagene | DNA polymerase mutants with reverse transcriptase activity |
| US20050123940A1 (en) * | 1999-10-29 | 2005-06-09 | Stratagene California | Compositions and methods for synthesizing cDNA |
| EP1452593B1 (en) * | 2001-11-14 | 2009-04-08 | Toyo Boseki Kabushiki Kaisha | Dna synthesis promoters, dna polymerase-associated factors and utilization thereof |
| AU2004230569B2 (en) | 2003-03-31 | 2007-12-06 | F. Hoffmann-La Roche Ag | Compositions and methods for detecting certain flaviviruses, including members of the Japanese encephalitis virus serogroup |
| US20050208482A1 (en) | 2004-03-16 | 2005-09-22 | Cohen David I | Tat-based immunomodulatory compositions and methods for their discovery and use |
| US8962293B2 (en) | 2006-10-18 | 2015-02-24 | Roche Molecular Systems, Inc. | DNA polymerases and related methods |
| JP5692933B2 (ja) | 2009-03-23 | 2015-04-01 | ナニアールエックス セラピューティックス インコーポレイテッド | 免疫刺激ＨＩＶＴａｔ誘導体ポリペプチドによる腫瘍治療 |
| EP2582803B1 (en) | 2010-06-18 | 2015-02-25 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| WO2011157436A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| CN103003418B (zh) | 2010-06-18 | 2015-08-26 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| ES2536252T3 (es) | 2010-06-18 | 2015-05-21 | F. Hoffmann-La Roche Ag | ADN polimerasas con diferenciación de desapareamientos 3' aumentada |
| CN103025869B (zh) | 2010-06-18 | 2015-07-01 | 霍夫曼-拉罗奇有限公司 | 具有增强的3’-错配辨别力的dna聚合酶 |
| EP2582806B1 (en) | 2010-06-18 | 2015-12-16 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| WO2011157434A1 (en) | 2010-06-18 | 2011-12-22 | Roche Diagnostics Gmbh | Dna polymerases with increased 3'-mismatch discrimination |
| ES2564692T3 (es) | 2010-06-18 | 2016-03-28 | F. Hoffmann-La Roche Ag | ADN polimerasas con discriminación incrementada de no correspondencia 3' |
| EP2675897B1 (en) | 2011-02-15 | 2016-05-11 | Roche Diagnostics GmbH | Dna polymerases with increased 3'-mismatch discrimination |
| ES2561885T3 (es) | 2011-04-11 | 2016-03-01 | F. Hoffmann-La Roche Ag | ADN polimerasas de actividad mejorada |
| CA2839964C (en) | 2011-07-28 | 2019-03-12 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
| JP6144697B2 (ja) | 2011-12-08 | 2017-06-07 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 改善された活性を有するｄｎａポリメラーゼ |
| EP2788480B1 (en) | 2011-12-08 | 2019-01-16 | Roche Diagnostics GmbH | Dna polymerases with improved activity |
| WO2013083263A1 (en) | 2011-12-08 | 2013-06-13 | Roche Diagnostics Gmbh | Dna polymerases with improved activity |
| WO2015051245A1 (en) | 2013-10-04 | 2015-04-09 | Pin Pharma, Inc. | Immunostimulatory hiv tat derivative polypeptides for use in cancer treatment |
| ES2969883T3 (es) | 2018-03-21 | 2024-05-23 | Hoffmann La Roche | ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados |
| JP7504872B2 (ja) | 2018-09-13 | 2024-06-24 | エフ． ホフマン－ラ ロシュ アーゲー | 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ |
| WO2026153862A1 (en) | 2025-01-14 | 2026-07-23 | F. Hoffmann-La Roche Ag | Dna polymerases with improved activity |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5374553A (en) * | 1986-08-22 | 1994-12-20 | Hoffmann-La Roche Inc. | DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima |
| US5912155A (en) * | 1994-09-30 | 1999-06-15 | Life Technologies, Inc. | Cloned DNA polymerases from Thermotoga neapolitana |
-
1996
- 1996-10-03 EP EP96115873A patent/EP0834569A1/en not_active Withdrawn
-
1997
- 1997-10-01 JP JP51624298A patent/JP4005637B2/ja not_active Expired - Fee Related
- 1997-10-01 DE DE69737502T patent/DE69737502T2/de not_active Expired - Lifetime
- 1997-10-01 NZ NZ334652A patent/NZ334652A/xx not_active IP Right Cessation
- 1997-10-01 AU AU50495/98A patent/AU717009B2/en not_active Ceased
- 1997-10-01 WO PCT/EP1997/005391 patent/WO1998014589A1/en not_active Ceased
- 1997-10-01 US US09/269,861 patent/US6468775B1/en not_active Expired - Lifetime
- 1997-10-01 RU RU99109121/13A patent/RU2197524C2/ru not_active IP Right Cessation
- 1997-10-01 IL IL129085A patent/IL129085A/en not_active IP Right Cessation
- 1997-10-01 CA CA002268014A patent/CA2268014C/en not_active Expired - Fee Related
- 1997-10-01 AT AT97913133T patent/ATE357527T1/de active
- 1997-10-01 EP EP97913133A patent/EP0929680B1/en not_active Expired - Lifetime
-
1999
- 1999-03-30 NO NO19991568A patent/NO323846B1/no not_active IP Right Cessation
Non-Patent Citations (1)
| Title |
|---|
| PERLER F.B et al. Thermostable DNA polymerases Advances in protein Chemistry, 1996, v.48, p.377-435. * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111733145A (zh) * | 2020-07-01 | 2020-10-02 | 济南国科医工科技发展有限公司 | 重组酶的纯化方法 |
| RU2809366C1 (ru) * | 2022-12-28 | 2023-12-11 | Федеральное бюджетное учреждение науки "Центральный научно-исследовательский институт эпидемиологии" Федеральной службы по надзору в сфере защиты прав потребителей и благополучия человека (ФБУН ЦНИИ Эпидемиологии Роспотребнадзора) | Способ получения большого фрагмента Bst-полимеразы (варианты) |
| RU2815455C1 (ru) * | 2023-05-10 | 2024-03-18 | Федеральное государственное бюджетное учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук (ИХБФМ СО РАН) | Рекомбинантный штамм бактерий Escherichia coli Rosetta 2(DE3)/pET28c-Mau - продуцент ДНК-полимеразы А |
| RU2840236C1 (ru) * | 2024-07-26 | 2025-05-19 | Федеральное государственное бюджетное учреждение науки Институт химической биологии и фундаментальной медицины Сибирского отделения Российской академии наук (ИХБФМ СО РАН) | Рекомбинантный штамм бактерий Escherichia coli Rosetta 2(DE3)/pET28c-TstP36H-Sso7d - продуцент химерной термостабильной ДНК-полимеразы TstP36H-Sso7d из Thermococcus stetteri |
Also Published As
| Publication number | Publication date |
|---|---|
| NO323846B1 (no) | 2007-07-09 |
| CA2268014C (en) | 2008-01-15 |
| NO991568L (no) | 1999-06-02 |
| IL129085A (en) | 2006-12-10 |
| US6468775B1 (en) | 2002-10-22 |
| EP0834569A1 (en) | 1998-04-08 |
| JP2001502170A (ja) | 2001-02-20 |
| EP0929680B1 (en) | 2007-03-21 |
| WO1998014589A1 (en) | 1998-04-09 |
| DE69737502D1 (en) | 2007-05-03 |
| DE69737502T2 (de) | 2007-10-31 |
| EP0929680A1 (en) | 1999-07-21 |
| JP4005637B2 (ja) | 2007-11-07 |
| AU5049598A (en) | 1998-04-24 |
| CA2268014A1 (en) | 1998-04-09 |
| ATE357527T1 (de) | 2007-04-15 |
| NO991568D0 (no) | 1999-03-30 |
| NZ334652A (en) | 2000-03-27 |
| AU717009B2 (en) | 2000-03-16 |
| IL129085A0 (en) | 2000-02-17 |
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