CA2268014C - Thermostable dna polymerase from carboxydothermus hydrogenoformans - Google Patents

Thermostable dna polymerase from carboxydothermus hydrogenoformans Download PDF

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Publication number
CA2268014C
CA2268014C CA002268014A CA2268014A CA2268014C CA 2268014 C CA2268014 C CA 2268014C CA 002268014 A CA002268014 A CA 002268014A CA 2268014 A CA2268014 A CA 2268014A CA 2268014 C CA2268014 C CA 2268014C
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CA
Canada
Prior art keywords
dna
leu
polymerase
glu
lys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA002268014A
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English (en)
French (fr)
Other versions
CA2268014A1 (en
Inventor
Waltraud Ankenbauer
Ursula Markau
Vitaly Svetlichny
Gudrun Schmitz-Agheguian
Astrid Reiser
Bernhard Angerer
Christine Ebenbichler
Frank Laue
Elizaveta Bonch-Osmolovskaya
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Roche Diagnostics GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH filed Critical Roche Diagnostics GmbH
Publication of CA2268014A1 publication Critical patent/CA2268014A1/en
Application granted granted Critical
Publication of CA2268014C publication Critical patent/CA2268014C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
CA002268014A 1996-10-03 1997-10-01 Thermostable dna polymerase from carboxydothermus hydrogenoformans Expired - Fee Related CA2268014C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP96115873.0 1996-10-03
EP96115873A EP0834569A1 (en) 1996-10-03 1996-10-03 Thermostable DNA polymerase from carboxydothermus hydrogenoformans
PCT/EP1997/005391 WO1998014589A1 (en) 1996-10-03 1997-10-01 Thermostable dna polymerase from carboxydothermus hydrogenoformans

Publications (2)

Publication Number Publication Date
CA2268014A1 CA2268014A1 (en) 1998-04-09
CA2268014C true CA2268014C (en) 2008-01-15

Family

ID=8223262

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002268014A Expired - Fee Related CA2268014C (en) 1996-10-03 1997-10-01 Thermostable dna polymerase from carboxydothermus hydrogenoformans

Country Status (12)

Country Link
US (1) US6468775B1 (enExample)
EP (2) EP0834569A1 (enExample)
JP (1) JP4005637B2 (enExample)
AT (1) ATE357527T1 (enExample)
AU (1) AU717009B2 (enExample)
CA (1) CA2268014C (enExample)
DE (1) DE69737502T2 (enExample)
IL (1) IL129085A (enExample)
NO (1) NO323846B1 (enExample)
NZ (1) NZ334652A (enExample)
RU (1) RU2197524C2 (enExample)
WO (1) WO1998014589A1 (enExample)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0921196A1 (en) * 1997-12-02 1999-06-09 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
EP0922765B1 (en) * 1997-12-02 2005-11-23 Roche Diagnostics GmbH Modified DNA-polymerase from carboxydothermus hydrogenoformans and its use for coupled reverse transcription and polymerase chain reaction
US20040009486A1 (en) * 1999-10-29 2004-01-15 Sorge Joseph A. Compositions and methods utilizing DNA polymerases
US20030228616A1 (en) * 1999-10-29 2003-12-11 Stratagene DNA polymerase mutants with reverse transcriptase activity
US20050123940A1 (en) * 1999-10-29 2005-06-09 Stratagene California Compositions and methods for synthesizing cDNA
EP1452593B1 (en) * 2001-11-14 2009-04-08 Toyo Boseki Kabushiki Kaisha Dna synthesis promoters, dna polymerase-associated factors and utilization thereof
AU2004230569B2 (en) 2003-03-31 2007-12-06 F. Hoffmann-La Roche Ag Compositions and methods for detecting certain flaviviruses, including members of the Japanese encephalitis virus serogroup
US20050208482A1 (en) 2004-03-16 2005-09-22 Cohen David I Tat-based immunomodulatory compositions and methods for their discovery and use
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
JP5692933B2 (ja) 2009-03-23 2015-04-01 ナニアールエックス セラピューティックス インコーポレイテッド 免疫刺激ＨＩＶＴａｔ誘導体ポリペプチドによる腫瘍治療
EP2582803B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
CN103003418B (zh) 2010-06-18 2015-08-26 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
CN103025869B (zh) 2010-06-18 2015-07-01 霍夫曼-拉罗奇有限公司 具有增强的3’-错配辨别力的dna聚合酶
EP2582806B1 (en) 2010-06-18 2015-12-16 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
WO2011157434A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
ES2564692T3 (es) 2010-06-18 2016-03-28 F. Hoffmann-La Roche Ag ADN polimerasas con discriminación incrementada de no correspondencia 3'
EP2675897B1 (en) 2011-02-15 2016-05-11 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
ES2561885T3 (es) 2011-04-11 2016-03-01 F. Hoffmann-La Roche Ag ADN polimerasas de actividad mejorada
CA2839964C (en) 2011-07-28 2019-03-12 F. Hoffmann-La Roche Ag Dna polymerases with improved activity
JP6144697B2 (ja) 2011-12-08 2017-06-07 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 改善された活性を有するｄｎａポリメラーゼ
EP2788480B1 (en) 2011-12-08 2019-01-16 Roche Diagnostics GmbH Dna polymerases with improved activity
WO2013083263A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
WO2015051245A1 (en) 2013-10-04 2015-04-09 Pin Pharma, Inc. Immunostimulatory hiv tat derivative polypeptides for use in cancer treatment
ES2969883T3 (es) 2018-03-21 2024-05-23 Hoffmann La Roche ADN polimerasas para la incorporación eficiente y eficaz de dNTP metilados
JP7504872B2 (ja) 2018-09-13 2024-06-24 エフ． ホフマン－ラ ロシュ アーゲー 改善されたストランド置換能力を有する変異体ｄｎａポリメラーゼ
CN111733145A (zh) * 2020-07-01 2020-10-02 济南国科医工科技发展有限公司 重组酶的纯化方法
WO2026153862A1 (en) 2025-01-14 2026-07-23 F. Hoffmann-La Roche Ag Dna polymerases with improved activity

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5374553A (en) * 1986-08-22 1994-12-20 Hoffmann-La Roche Inc. DNA encoding a thermostable nucleic acid polymerase enzyme from thermotoga maritima
US5912155A (en) * 1994-09-30 1999-06-15 Life Technologies, Inc. Cloned DNA polymerases from Thermotoga neapolitana

Also Published As

Publication number Publication date
NO323846B1 (no) 2007-07-09
NO991568L (no) 1999-06-02
IL129085A (en) 2006-12-10
US6468775B1 (en) 2002-10-22
EP0834569A1 (en) 1998-04-08
JP2001502170A (ja) 2001-02-20
EP0929680B1 (en) 2007-03-21
WO1998014589A1 (en) 1998-04-09
DE69737502D1 (en) 2007-05-03
DE69737502T2 (de) 2007-10-31
EP0929680A1 (en) 1999-07-21
JP4005637B2 (ja) 2007-11-07
RU2197524C2 (ru) 2003-01-27
AU5049598A (en) 1998-04-24
CA2268014A1 (en) 1998-04-09
ATE357527T1 (de) 2007-04-15
NO991568D0 (no) 1999-03-30
NZ334652A (en) 2000-03-27
AU717009B2 (en) 2000-03-16
IL129085A0 (en) 2000-02-17

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EP0929680B1 (en) Thermostable dna polymerase from carboxydothermus hydrogenoformans
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