RU2015122802A - Клонированное животное, отличное от человека, которое не содержит селективный маркер - Google Patents
Клонированное животное, отличное от человека, которое не содержит селективный маркер Download PDFInfo
- Publication number
- RU2015122802A RU2015122802A RU2015122802A RU2015122802A RU2015122802A RU 2015122802 A RU2015122802 A RU 2015122802A RU 2015122802 A RU2015122802 A RU 2015122802A RU 2015122802 A RU2015122802 A RU 2015122802A RU 2015122802 A RU2015122802 A RU 2015122802A
- Authority
- RU
- Russia
- Prior art keywords
- promoter
- recombinase
- somatic cell
- cells
- genetically modified
- Prior art date
Links
- 239000003550 marker Substances 0.000 title claims 24
- 241001465754 Metazoa Species 0.000 title claims 6
- 210000001082 somatic cell Anatomy 0.000 claims 32
- 108090000623 proteins and genes Proteins 0.000 claims 31
- 108010091086 Recombinases Proteins 0.000 claims 22
- 102000018120 Recombinases Human genes 0.000 claims 21
- 108010052160 Site-specific recombinase Proteins 0.000 claims 20
- 210000000287 oocyte Anatomy 0.000 claims 19
- 230000006798 recombination Effects 0.000 claims 18
- 238000005215 recombination Methods 0.000 claims 18
- 210000004027 cell Anatomy 0.000 claims 12
- 239000002773 nucleotide Substances 0.000 claims 12
- 125000003729 nucleotide group Chemical group 0.000 claims 12
- 238000000034 method Methods 0.000 claims 11
- 108700028369 Alleles Proteins 0.000 claims 9
- 241000282898 Sus scrofa Species 0.000 claims 7
- 230000000415 inactivating effect Effects 0.000 claims 7
- 210000002950 fibroblast Anatomy 0.000 claims 6
- 108020004707 nucleic acids Proteins 0.000 claims 5
- 150000007523 nucleic acids Chemical class 0.000 claims 5
- 102000039446 nucleic acids Human genes 0.000 claims 5
- 210000002459 blastocyst Anatomy 0.000 claims 4
- 230000035897 transcription Effects 0.000 claims 4
- 238000013518 transcription Methods 0.000 claims 4
- 241000283690 Bos taurus Species 0.000 claims 3
- 241000282412 Homo Species 0.000 claims 3
- 101710135898 Myc proto-oncogene protein Proteins 0.000 claims 3
- 102100038895 Myc proto-oncogene protein Human genes 0.000 claims 3
- 101100247004 Rattus norvegicus Qsox1 gene Proteins 0.000 claims 3
- 101710150448 Transcriptional regulator Myc Proteins 0.000 claims 3
- 101150111214 lin-28 gene Proteins 0.000 claims 3
- 210000001789 adipocyte Anatomy 0.000 claims 2
- 210000000601 blood cell Anatomy 0.000 claims 2
- 210000002449 bone cell Anatomy 0.000 claims 2
- 210000005229 liver cell Anatomy 0.000 claims 2
- 210000001161 mammalian embryo Anatomy 0.000 claims 2
- 210000000663 muscle cell Anatomy 0.000 claims 2
- 210000002569 neuron Anatomy 0.000 claims 2
- 210000001778 pluripotent stem cell Anatomy 0.000 claims 2
- 210000004927 skin cell Anatomy 0.000 claims 2
- 241000283707 Capra Species 0.000 claims 1
- 241000283074 Equus asinus Species 0.000 claims 1
- 241000283073 Equus caballus Species 0.000 claims 1
- 241000283973 Oryctolagus cuniculus Species 0.000 claims 1
- 241001494479 Pecora Species 0.000 claims 1
- 230000003213 activating effect Effects 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000002513 implantation Methods 0.000 claims 1
- 210000003292 kidney cell Anatomy 0.000 claims 1
- 210000004291 uterus Anatomy 0.000 claims 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0273—Cloned vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8771—Bovine embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8778—Swine embryos
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/20—Animal model comprising regulated expression system
- A01K2217/203—Animal model comprising inducible/conditional expression system, e.g. hormones, tet
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/007—Vectors comprising a special translation-regulating system cell or tissue specific
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2999/00—Further aspects of viruses or vectors not covered by groups C12N2710/00 - C12N2796/00 or C12N2800/00
- C12N2999/007—Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Public Health (AREA)
- Reproductive Health (AREA)
- Transplantation (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261730771P | 2012-11-28 | 2012-11-28 | |
| US61/730,771 | 2012-11-28 | ||
| PCT/US2013/072088 WO2014085501A1 (en) | 2012-11-28 | 2013-11-26 | Cloned non-human animals free of selective markers |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2015122802A true RU2015122802A (ru) | 2017-01-10 |
Family
ID=49725406
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015122802A RU2015122802A (ru) | 2012-11-28 | 2013-11-26 | Клонированное животное, отличное от человека, которое не содержит селективный маркер |
Country Status (16)
| Country | Link |
|---|---|
| US (1) | US20140221734A1 (enExample) |
| EP (1) | EP2925122A1 (enExample) |
| JP (2) | JP6154479B2 (enExample) |
| KR (1) | KR20150089004A (enExample) |
| CN (1) | CN104918484A (enExample) |
| AU (1) | AU2013352235A1 (enExample) |
| BR (1) | BR112015012408A2 (enExample) |
| CA (1) | CA2891163A1 (enExample) |
| HK (1) | HK1211177A1 (enExample) |
| IL (1) | IL238771A0 (enExample) |
| MX (1) | MX353279B (enExample) |
| MY (1) | MY193790A (enExample) |
| RU (1) | RU2015122802A (enExample) |
| SG (1) | SG11201503752SA (enExample) |
| WO (1) | WO2014085501A1 (enExample) |
| ZA (1) | ZA201503305B (enExample) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3988649B1 (en) | 2013-09-18 | 2024-11-27 | Kymab Limited | Methods, cells and organisms |
| JP6929527B2 (ja) * | 2015-03-04 | 2021-09-01 | 株式会社ポル・メド・テック | 安定した表現型を示す疾患モデルブタおよびその作製方法 |
| CN105200086A (zh) * | 2015-10-22 | 2015-12-30 | 中国农业科学院北京畜牧兽医研究所 | 一种无选择标记的多基因复合性状基因工程猪的制备方法 |
| EP4249074A3 (en) * | 2015-11-04 | 2024-01-10 | Fate Therapeutics, Inc. | Genomic engineering of pluripotent cells |
| CA3099441A1 (en) * | 2018-06-13 | 2019-12-19 | Regeneron Pharmaceuticals, Inc. | A rodent model of fibrodysplasia ossificans progressiva |
| CN109504708A (zh) * | 2018-12-03 | 2019-03-22 | 江苏集萃药康生物科技有限公司 | 一种筛选标记自我删除的基因打靶载体及方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1375654A3 (en) * | 1998-11-02 | 2008-01-16 | GTC Biotherapeutics, Inc. | Transgenic and cloned mammals |
| US7049480B1 (en) * | 2000-09-01 | 2006-05-23 | Avigenics, Inc. | Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy |
| US7803920B2 (en) * | 2004-09-29 | 2010-09-28 | Shinya Yamanaka | ECAT16 gene expressed specifically in ES cells and utilization of the same |
| WO2009121999A1 (es) * | 2008-04-04 | 2009-10-08 | Consejo Superior De Investigaciones Científicas | Promotor especifico de celulas troncales |
| US8518392B2 (en) * | 2009-08-14 | 2013-08-27 | Regeneron Pharmaceuticals, Inc. | Promoter-regulated differentiation-dependent self-deleting cassette |
| CN102628061A (zh) * | 2012-04-09 | 2012-08-08 | 西北农林科技大学 | 一种hbd3 乳腺特异性表达载体及构建的重组细胞 |
-
2013
- 2013-11-26 MX MX2015006810A patent/MX353279B/es active IP Right Grant
- 2013-11-26 HK HK15112184.4A patent/HK1211177A1/xx unknown
- 2013-11-26 EP EP13802213.2A patent/EP2925122A1/en not_active Withdrawn
- 2013-11-26 CN CN201380060720.0A patent/CN104918484A/zh active Pending
- 2013-11-26 RU RU2015122802A patent/RU2015122802A/ru not_active Application Discontinuation
- 2013-11-26 JP JP2015544195A patent/JP6154479B2/ja not_active Expired - Fee Related
- 2013-11-26 US US14/091,207 patent/US20140221734A1/en not_active Abandoned
- 2013-11-26 WO PCT/US2013/072088 patent/WO2014085501A1/en not_active Ceased
- 2013-11-26 MY MYPI2015001216A patent/MY193790A/en unknown
- 2013-11-26 CA CA2891163A patent/CA2891163A1/en not_active Abandoned
- 2013-11-26 AU AU2013352235A patent/AU2013352235A1/en not_active Abandoned
- 2013-11-26 KR KR1020157012263A patent/KR20150089004A/ko not_active Withdrawn
- 2013-11-26 BR BR112015012408A patent/BR112015012408A2/pt not_active IP Right Cessation
- 2013-11-26 SG SG11201503752SA patent/SG11201503752SA/en unknown
-
2015
- 2015-05-12 ZA ZA2015/03305A patent/ZA201503305B/en unknown
- 2015-05-12 IL IL238771A patent/IL238771A0/en unknown
-
2016
- 2016-11-22 JP JP2016226816A patent/JP6227747B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| SG11201503752SA (en) | 2015-06-29 |
| IL238771A0 (en) | 2015-06-30 |
| BR112015012408A2 (pt) | 2017-07-11 |
| JP6154479B2 (ja) | 2017-06-28 |
| JP2016502407A (ja) | 2016-01-28 |
| JP2017060502A (ja) | 2017-03-30 |
| AU2013352235A1 (en) | 2015-05-28 |
| KR20150089004A (ko) | 2015-08-04 |
| ZA201503305B (en) | 2016-05-25 |
| CN104918484A (zh) | 2015-09-16 |
| US20140221734A1 (en) | 2014-08-07 |
| HK1211177A1 (en) | 2016-05-20 |
| CA2891163A1 (en) | 2014-06-05 |
| MY193790A (en) | 2022-10-27 |
| JP6227747B2 (ja) | 2017-11-08 |
| WO2014085501A1 (en) | 2014-06-05 |
| EP2925122A1 (en) | 2015-10-07 |
| MX2015006810A (es) | 2015-09-07 |
| MX353279B (es) | 2018-01-05 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA94 | Acknowledgement of application withdrawn (non-payment of fees) |
Effective date: 20190626 |