CN104918484A - 不含选择性标志的克隆的非人类动物 - Google Patents

不含选择性标志的克隆的非人类动物 Download PDF

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Publication number
CN104918484A
CN104918484A CN201380060720.0A CN201380060720A CN104918484A CN 104918484 A CN104918484 A CN 104918484A CN 201380060720 A CN201380060720 A CN 201380060720A CN 104918484 A CN104918484 A CN 104918484A
Authority
CN
China
Prior art keywords
promoter
recombinase
gene
selectable marker
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN201380060720.0A
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English (en)
Chinese (zh)
Inventor
G·C·龚
K·V·莱
D·M·瓦伦泽拉
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Regeneron Pharmaceuticals Inc
Original Assignee
Regeneron Pharmaceuticals Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49725406&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CN104918484(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharmaceuticals Inc filed Critical Regeneron Pharmaceuticals Inc
Publication of CN104918484A publication Critical patent/CN104918484A/zh
Pending legal-status Critical Current

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/203—Animal model comprising inducible/conditional expression system, e.g. hormones, tet
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/007—Vectors comprising a special translation-regulating system cell or tissue specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2999/00—Further aspects of viruses or vectors not covered by groups C12N2710/00 - C12N2796/00 or C12N2800/00
    • C12N2999/007—Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN201380060720.0A 2012-11-28 2013-11-26 不含选择性标志的克隆的非人类动物 Pending CN104918484A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261730771P 2012-11-28 2012-11-28
US61/730,771 2012-11-28
PCT/US2013/072088 WO2014085501A1 (en) 2012-11-28 2013-11-26 Cloned non-human animals free of selective markers

Publications (1)

Publication Number Publication Date
CN104918484A true CN104918484A (zh) 2015-09-16

Family

ID=49725406

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201380060720.0A Pending CN104918484A (zh) 2012-11-28 2013-11-26 不含选择性标志的克隆的非人类动物

Country Status (16)

Country Link
US (1) US20140221734A1 (enExample)
EP (1) EP2925122A1 (enExample)
JP (2) JP6154479B2 (enExample)
KR (1) KR20150089004A (enExample)
CN (1) CN104918484A (enExample)
AU (1) AU2013352235A1 (enExample)
BR (1) BR112015012408A2 (enExample)
CA (1) CA2891163A1 (enExample)
HK (1) HK1211177A1 (enExample)
IL (1) IL238771A0 (enExample)
MX (1) MX353279B (enExample)
MY (1) MY193790A (enExample)
RU (1) RU2015122802A (enExample)
SG (1) SG11201503752SA (enExample)
WO (1) WO2014085501A1 (enExample)
ZA (1) ZA201503305B (enExample)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109504708A (zh) * 2018-12-03 2019-03-22 江苏集萃药康生物科技有限公司 一种筛选标记自我删除的基因打靶载体及方法
CN112292030A (zh) * 2018-06-13 2021-01-29 瑞泽恩制药公司 进行性骨化性纤维发育不良的啮齿类动物模型
CN115806940A (zh) * 2015-11-04 2023-03-17 菲特治疗公司 多能细胞的基因组工程改造

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3988649B1 (en) 2013-09-18 2024-11-27 Kymab Limited Methods, cells and organisms
WO2016140353A1 (ja) * 2015-03-04 2016-09-09 株式会社 医療実験用大動物供給事業準備会社 安定した表現型を示す疾患モデルブタおよびその作製方法
CN105200086A (zh) * 2015-10-22 2015-12-30 中国农业科学院北京畜牧兽医研究所 一种无选择标记的多基因复合性状基因工程猪的制备方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011020014A1 (en) * 2009-08-14 2011-02-17 Regeneron Pharmaceuticals, Inc. Promoter-regulated differentiation-dependent self-deleting cassette

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1375654A3 (en) * 1998-11-02 2008-01-16 GTC Biotherapeutics, Inc. Transgenic and cloned mammals
US7049480B1 (en) * 2000-09-01 2006-05-23 Avigenics, Inc. Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy
EP1811023A4 (en) * 2004-09-29 2009-04-29 Dainippon Sumitomo Pharma Co SPECIFICALLY EXPRESSED IN ES CELLS AND USE THEREOF
WO2009121999A1 (es) * 2008-04-04 2009-10-08 Consejo Superior De Investigaciones Científicas Promotor especifico de celulas troncales
CN102628061A (zh) * 2012-04-09 2012-08-08 西北农林科技大学 一种hbd3 乳腺特异性表达载体及构建的重组细胞

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2011020014A1 (en) * 2009-08-14 2011-02-17 Regeneron Pharmaceuticals, Inc. Promoter-regulated differentiation-dependent self-deleting cassette

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
KEISUKE OKITA，ET AL: "Generation of germline-competent induced pluripotent stem cells", 《NATURE》 *
LILI ET AL: "Production of a reporter transgenic pig for monitoring Cre recombinase activity", 《BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS》 *

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115806940A (zh) * 2015-11-04 2023-03-17 菲特治疗公司 多能细胞的基因组工程改造
CN112292030A (zh) * 2018-06-13 2021-01-29 瑞泽恩制药公司 进行性骨化性纤维发育不良的啮齿类动物模型
US12089576B2 (en) 2018-06-13 2024-09-17 Regeneron Pharmaceuticals, Inc. Nucleic acids comprising a modified rodent activin a receptor type 1 (Acvr1) gene
CN109504708A (zh) * 2018-12-03 2019-03-22 江苏集萃药康生物科技有限公司 一种筛选标记自我删除的基因打靶载体及方法

Also Published As

Publication number Publication date
IL238771A0 (en) 2015-06-30
HK1211177A1 (en) 2016-05-20
JP2017060502A (ja) 2017-03-30
MX2015006810A (es) 2015-09-07
BR112015012408A2 (pt) 2017-07-11
SG11201503752SA (en) 2015-06-29
JP2016502407A (ja) 2016-01-28
US20140221734A1 (en) 2014-08-07
MY193790A (en) 2022-10-27
EP2925122A1 (en) 2015-10-07
CA2891163A1 (en) 2014-06-05
MX353279B (es) 2018-01-05
KR20150089004A (ko) 2015-08-04
WO2014085501A1 (en) 2014-06-05
RU2015122802A (ru) 2017-01-10
JP6154479B2 (ja) 2017-06-28
AU2013352235A1 (en) 2015-05-28
JP6227747B2 (ja) 2017-11-08
ZA201503305B (en) 2016-05-25

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