MX2015006810A - Animales no humanos clonados libres de marcadores selectivos. - Google Patents

Animales no humanos clonados libres de marcadores selectivos.

Info

Publication number
MX2015006810A
MX2015006810A MX2015006810A MX2015006810A MX2015006810A MX 2015006810 A MX2015006810 A MX 2015006810A MX 2015006810 A MX2015006810 A MX 2015006810A MX 2015006810 A MX2015006810 A MX 2015006810A MX 2015006810 A MX2015006810 A MX 2015006810A
Authority
MX
Mexico
Prior art keywords
genetically modified
recombinase
human animal
somatic cells
free
Prior art date
Application number
MX2015006810A
Other languages
English (en)
Other versions
MX353279B (es
Inventor
David M Valenzuela
Guochun Gong
Ka-Man Venus Lai
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=49725406&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2015006810(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of MX2015006810A publication Critical patent/MX2015006810A/es
Publication of MX353279B publication Critical patent/MX353279B/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/203—Animal model comprising inducible/conditional expression system, e.g. hormones, tet
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/007—Vectors comprising a special translation-regulating system cell or tissue specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2999/00—Further aspects of viruses or vectors not covered by groups C12N2710/00 - C12N2796/00 or C12N2800/00
    • C12N2999/007—Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporcionan las células somáticas genéticamente modificadas de un animal no humano, que se modifican para contener un casete de expresión recombinasa, auto-escindible que comprende un gene recombinasa específico del sitio, unido operativamente a un promotor específico de la célula ES. Se proporcionan las composiciones y los métodos para producir un animal no humano donado, genéticamente modificado, que está libre de un gene marcador selectivo y un gene recombinasa, en donde una construcción objetivo comprende un gene recombinasa auto-escindible unido operativamente a un promotor específico de la célula ES que se introduce en las células somáticas diferenciadas. El genoma modificado genéticamente se las células somáticas se transfiere en un ovocito huésped enucleado. El cigoto creado artificialmente después se cultiva in vitro hasta la etapa embriónica de blastocisto y se implanta subsecuentemente en un útero de una madre sustituta para formar un animal no humano donado, modificado genéticamente, libre de los genes de marcador selectivo y los genes recombinasa.
MX2015006810A 2012-11-28 2013-11-26 Animales no humanos clonados libres de marcadores selectivos. MX353279B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261730771P 2012-11-28 2012-11-28
PCT/US2013/072088 WO2014085501A1 (en) 2012-11-28 2013-11-26 Cloned non-human animals free of selective markers

Publications (2)

Publication Number Publication Date
MX2015006810A true MX2015006810A (es) 2015-09-07
MX353279B MX353279B (es) 2018-01-05

Family

ID=49725406

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2015006810A MX353279B (es) 2012-11-28 2013-11-26 Animales no humanos clonados libres de marcadores selectivos.

Country Status (16)

Country Link
US (1) US20140221734A1 (es)
EP (1) EP2925122A1 (es)
JP (2) JP6154479B2 (es)
KR (1) KR20150089004A (es)
CN (1) CN104918484A (es)
AU (1) AU2013352235A1 (es)
BR (1) BR112015012408A2 (es)
CA (1) CA2891163A1 (es)
HK (1) HK1211177A1 (es)
IL (1) IL238771A0 (es)
MX (1) MX353279B (es)
MY (1) MY193790A (es)
RU (1) RU2015122802A (es)
SG (1) SG11201503752SA (es)
WO (1) WO2014085501A1 (es)
ZA (1) ZA201503305B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE202014010413U1 (de) * 2013-09-18 2015-12-08 Kymab Limited Zellen und Organismen
JP6929527B2 (ja) 2015-03-04 2021-09-01 株式会社ポル・メド・テック 安定した表現型を示す疾患モデルブタおよびその作製方法
CN105200086A (zh) * 2015-10-22 2015-12-30 中国农业科学院北京畜牧兽医研究所 一种无选择标记的多基因复合性状基因工程猪的制备方法
CN109504708A (zh) * 2018-12-03 2019-03-22 江苏集萃药康生物科技有限公司 一种筛选标记自我删除的基因打靶载体及方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1375654A3 (en) * 1998-11-02 2008-01-16 GTC Biotherapeutics, Inc. Transgenic and cloned mammals
US7049480B1 (en) * 2000-09-01 2006-05-23 Avigenics, Inc. Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy
US7803920B2 (en) * 2004-09-29 2010-09-28 Shinya Yamanaka ECAT16 gene expressed specifically in ES cells and utilization of the same
WO2009121999A1 (es) * 2008-04-04 2009-10-08 Consejo Superior De Investigaciones Científicas Promotor especifico de celulas troncales
WO2011020005A1 (en) * 2009-08-14 2011-02-17 Regeneron Pharmaceuticals, Inc. miRNA-REGULATED DIFFERENTIATION-DEPENDENT SELF-DELETING CASSETTE
CN102628061A (zh) * 2012-04-09 2012-08-08 西北农林科技大学 一种hbd3 乳腺特异性表达载体及构建的重组细胞

Also Published As

Publication number Publication date
SG11201503752SA (en) 2015-06-29
IL238771A0 (en) 2015-06-30
BR112015012408A2 (pt) 2017-07-11
CN104918484A (zh) 2015-09-16
CA2891163A1 (en) 2014-06-05
RU2015122802A (ru) 2017-01-10
JP6154479B2 (ja) 2017-06-28
WO2014085501A1 (en) 2014-06-05
JP2016502407A (ja) 2016-01-28
JP2017060502A (ja) 2017-03-30
KR20150089004A (ko) 2015-08-04
ZA201503305B (en) 2016-05-25
AU2013352235A1 (en) 2015-05-28
HK1211177A1 (en) 2016-05-20
MX353279B (es) 2018-01-05
JP6227747B2 (ja) 2017-11-08
EP2925122A1 (en) 2015-10-07
MY193790A (en) 2022-10-27
US20140221734A1 (en) 2014-08-07

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