MX2015006810A - Animales no humanos clonados libres de marcadores selectivos. - Google Patents
Animales no humanos clonados libres de marcadores selectivos.Info
- Publication number
- MX2015006810A MX2015006810A MX2015006810A MX2015006810A MX2015006810A MX 2015006810 A MX2015006810 A MX 2015006810A MX 2015006810 A MX2015006810 A MX 2015006810A MX 2015006810 A MX2015006810 A MX 2015006810A MX 2015006810 A MX2015006810 A MX 2015006810A
- Authority
- MX
- Mexico
- Prior art keywords
- genetically modified
- recombinase
- human animal
- somatic cells
- free
- Prior art date
Links
- 108010091086 Recombinases Proteins 0.000 abstract 4
- 210000001082 somatic cell Anatomy 0.000 abstract 3
- 210000004027 cell Anatomy 0.000 abstract 2
- 239000003550 marker Substances 0.000 abstract 2
- 102000018120 Recombinases Human genes 0.000 abstract 1
- 108010052160 Site-specific recombinase Proteins 0.000 abstract 1
- 210000002459 blastocyst Anatomy 0.000 abstract 1
- 238000000338 in vitro Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 210000000287 oocyte Anatomy 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 230000008685 targeting Effects 0.000 abstract 1
- 210000004291 uterus Anatomy 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0273—Cloned vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D19/00—Instruments or methods for reproduction or fertilisation
- A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8771—Bovine embryos
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
- C12N15/877—Techniques for producing new mammalian cloned embryos
- C12N15/8778—Swine embryos
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/20—Animal model comprising regulated expression system
- A01K2217/203—Animal model comprising inducible/conditional expression system, e.g. hormones, tet
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/007—Vectors comprising a special translation-regulating system cell or tissue specific
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2999/00—Further aspects of viruses or vectors not covered by groups C12N2710/00 - C12N2796/00 or C12N2800/00
- C12N2999/007—Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Veterinary Medicine (AREA)
- Wood Science & Technology (AREA)
- Animal Behavior & Ethology (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Molecular Biology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Transplantation (AREA)
- Reproductive Health (AREA)
- Public Health (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Se proporcionan las células somáticas genéticamente modificadas de un animal no humano, que se modifican para contener un casete de expresión recombinasa, auto-escindible que comprende un gene recombinasa específico del sitio, unido operativamente a un promotor específico de la célula ES. Se proporcionan las composiciones y los métodos para producir un animal no humano donado, genéticamente modificado, que está libre de un gene marcador selectivo y un gene recombinasa, en donde una construcción objetivo comprende un gene recombinasa auto-escindible unido operativamente a un promotor específico de la célula ES que se introduce en las células somáticas diferenciadas. El genoma modificado genéticamente se las células somáticas se transfiere en un ovocito huésped enucleado. El cigoto creado artificialmente después se cultiva in vitro hasta la etapa embriónica de blastocisto y se implanta subsecuentemente en un útero de una madre sustituta para formar un animal no humano donado, modificado genéticamente, libre de los genes de marcador selectivo y los genes recombinasa.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201261730771P | 2012-11-28 | 2012-11-28 | |
PCT/US2013/072088 WO2014085501A1 (en) | 2012-11-28 | 2013-11-26 | Cloned non-human animals free of selective markers |
Publications (2)
Publication Number | Publication Date |
---|---|
MX2015006810A true MX2015006810A (es) | 2015-09-07 |
MX353279B MX353279B (es) | 2018-01-05 |
Family
ID=49725406
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
MX2015006810A MX353279B (es) | 2012-11-28 | 2013-11-26 | Animales no humanos clonados libres de marcadores selectivos. |
Country Status (16)
Country | Link |
---|---|
US (1) | US20140221734A1 (es) |
EP (1) | EP2925122A1 (es) |
JP (2) | JP6154479B2 (es) |
KR (1) | KR20150089004A (es) |
CN (1) | CN104918484A (es) |
AU (1) | AU2013352235A1 (es) |
BR (1) | BR112015012408A2 (es) |
CA (1) | CA2891163A1 (es) |
HK (1) | HK1211177A1 (es) |
IL (1) | IL238771A0 (es) |
MX (1) | MX353279B (es) |
MY (1) | MY193790A (es) |
RU (1) | RU2015122802A (es) |
SG (1) | SG11201503752SA (es) |
WO (1) | WO2014085501A1 (es) |
ZA (1) | ZA201503305B (es) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2681622T3 (es) | 2013-09-18 | 2018-09-14 | Kymab Limited | Métodos, células y organismos |
WO2016140353A1 (ja) * | 2015-03-04 | 2016-09-09 | 株式会社 医療実験用大動物供給事業準備会社 | 安定した表現型を示す疾患モデルブタおよびその作製方法 |
CN105200086A (zh) * | 2015-10-22 | 2015-12-30 | 中国农业科学院北京畜牧兽医研究所 | 一种无选择标记的多基因复合性状基因工程猪的制备方法 |
SG11202010891SA (en) * | 2018-06-13 | 2020-11-27 | Regeneron Pharma | A rodent model of fibrodysplasia ossificans progressiva |
CN109504708A (zh) * | 2018-12-03 | 2019-03-22 | 江苏集萃药康生物科技有限公司 | 一种筛选标记自我删除的基因打靶载体及方法 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP1375654A3 (en) * | 1998-11-02 | 2008-01-16 | GTC Biotherapeutics, Inc. | Transgenic and cloned mammals |
US7049480B1 (en) * | 2000-09-01 | 2006-05-23 | Avigenics, Inc. | Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy |
WO2006035741A1 (ja) * | 2004-09-29 | 2006-04-06 | Dainippon Sumitomo Pharma Co., Ltd. | Es細胞特異的発現遺伝子及びその利用 |
WO2009121999A1 (es) * | 2008-04-04 | 2009-10-08 | Consejo Superior De Investigaciones Científicas | Promotor especifico de celulas troncales |
US8354389B2 (en) * | 2009-08-14 | 2013-01-15 | Regeneron Pharmaceuticals, Inc. | miRNA-regulated differentiation-dependent self-deleting cassette |
CN102628061A (zh) * | 2012-04-09 | 2012-08-08 | 西北农林科技大学 | 一种hbd3 乳腺特异性表达载体及构建的重组细胞 |
-
2013
- 2013-11-26 EP EP13802213.2A patent/EP2925122A1/en not_active Withdrawn
- 2013-11-26 MX MX2015006810A patent/MX353279B/es active IP Right Grant
- 2013-11-26 BR BR112015012408A patent/BR112015012408A2/pt not_active IP Right Cessation
- 2013-11-26 KR KR1020157012263A patent/KR20150089004A/ko not_active Application Discontinuation
- 2013-11-26 CA CA2891163A patent/CA2891163A1/en not_active Abandoned
- 2013-11-26 JP JP2015544195A patent/JP6154479B2/ja not_active Expired - Fee Related
- 2013-11-26 CN CN201380060720.0A patent/CN104918484A/zh active Pending
- 2013-11-26 AU AU2013352235A patent/AU2013352235A1/en not_active Abandoned
- 2013-11-26 US US14/091,207 patent/US20140221734A1/en not_active Abandoned
- 2013-11-26 SG SG11201503752SA patent/SG11201503752SA/en unknown
- 2013-11-26 MY MYPI2015001216A patent/MY193790A/en unknown
- 2013-11-26 WO PCT/US2013/072088 patent/WO2014085501A1/en active Application Filing
- 2013-11-26 RU RU2015122802A patent/RU2015122802A/ru not_active Application Discontinuation
-
2015
- 2015-05-12 ZA ZA2015/03305A patent/ZA201503305B/en unknown
- 2015-05-12 IL IL238771A patent/IL238771A0/en unknown
- 2015-12-10 HK HK15112184.4A patent/HK1211177A1/xx unknown
-
2016
- 2016-11-22 JP JP2016226816A patent/JP6227747B2/ja not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
JP2017060502A (ja) | 2017-03-30 |
MY193790A (en) | 2022-10-27 |
KR20150089004A (ko) | 2015-08-04 |
AU2013352235A1 (en) | 2015-05-28 |
HK1211177A1 (en) | 2016-05-20 |
CA2891163A1 (en) | 2014-06-05 |
CN104918484A (zh) | 2015-09-16 |
RU2015122802A (ru) | 2017-01-10 |
JP6227747B2 (ja) | 2017-11-08 |
JP2016502407A (ja) | 2016-01-28 |
IL238771A0 (en) | 2015-06-30 |
EP2925122A1 (en) | 2015-10-07 |
MX353279B (es) | 2018-01-05 |
WO2014085501A1 (en) | 2014-06-05 |
BR112015012408A2 (pt) | 2017-07-11 |
JP6154479B2 (ja) | 2017-06-28 |
SG11201503752SA (en) | 2015-06-29 |
US20140221734A1 (en) | 2014-08-07 |
ZA201503305B (en) | 2016-05-25 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
FG | Grant or registration |