MX2018000071A - Musculo cardiaco humanizado. - Google Patents

Musculo cardiaco humanizado.

Info

Publication number
MX2018000071A
MX2018000071A MX2018000071A MX2018000071A MX2018000071A MX 2018000071 A MX2018000071 A MX 2018000071A MX 2018000071 A MX2018000071 A MX 2018000071A MX 2018000071 A MX2018000071 A MX 2018000071A MX 2018000071 A MX2018000071 A MX 2018000071A
Authority
MX
Mexico
Prior art keywords
human
handii
nkx2
tbx5
combination
Prior art date
Application number
MX2018000071A
Other languages
English (en)
Inventor
J Garry Daniel
G Garry Mary
Koyano-Nakagawa Naoko
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Publication of MX2018000071A publication Critical patent/MX2018000071A/es

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0271—Chimeric animals, e.g. comprising exogenous cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knockout animals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

Se describe en la presente un método para producir un animal quimérico no humano que expresa un gen humano de NKX2- 5, HANDII, TBX5 o una combinación de los mismos que comprende: a) generar un NKX2-5, HANDII, TBX5 o una combinación de los mismos de una célula animal no humana, en la que ambas copias del gen NKX2-5, HANDII, TBX5 no humano o una combinación de las mismas llevan una mutación que impide la producción de proteína funcional NKX2-5, HANDII, TBX5 o una combinación de las mismas en el animal no humano; b) crear un NKX2-5, HANDII, TBX5 o una combinación de los mismos blastocito no humano mediante transferencia nuclear de células somáticas que comprende fusionar un núcleo del NKX2- 5, HANDII, TBX5 o una combinación de las mismas células animales no humanas de a) en un no nucleado enucleado ovocito humano y activación del ovocito para dividirse para formar un NKX2-5, HANDII, TBX5 o una combinación de los mismos blastocito no humano no humano; c) introducir células madre humanas en el NKX2-5, HANDII, TBX5 o combinación de blastocito nulo no humano de b); y d) implantar el blastocito de c) en un animal no humano sustituto pseudopreñado para generar un animal no humano quimérico que expresa NKX2-5, HANDII, TBX5 humano o una combinación de los mismos.
MX2018000071A 2015-06-30 2016-06-30 Musculo cardiaco humanizado. MX2018000071A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562187040P 2015-06-30 2015-06-30
PCT/US2016/040431 WO2017004388A1 (en) 2015-06-30 2016-06-30 Humanized heart muscle

Publications (1)

Publication Number Publication Date
MX2018000071A true MX2018000071A (es) 2018-03-16

Family

ID=57609529

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018000071A MX2018000071A (es) 2015-06-30 2016-06-30 Musculo cardiaco humanizado.

Country Status (13)

Country Link
US (2) US10897880B2 (es)
EP (1) EP3316899A4 (es)
JP (1) JP2018523999A (es)
KR (1) KR20180021135A (es)
CN (1) CN108472318A (es)
AU (1) AU2016288671A1 (es)
BR (1) BR112017028608A2 (es)
CA (1) CA2991056A1 (es)
CO (1) CO2018000859A2 (es)
IL (1) IL256544A (es)
MX (1) MX2018000071A (es)
RU (1) RU2018103234A (es)
WO (1) WO2017004388A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017018932A2 (pt) 2015-03-03 2018-07-31 Univ Minnesota etv2 e usos do mesmo
CA2991053A1 (en) 2015-06-30 2017-01-05 Regents Of The University Of Minnesota Humanized skeletal muscle
JP2018523999A (ja) 2015-06-30 2018-08-30 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ ヒト化心筋

Family Cites Families (28)

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NZ326996A (en) * 1996-01-09 1998-11-25 Univ California Sustained culture of pluripotent ungulate embryonic stem-like cells, methods of making and using the cells to produce transgenic ungulates
US5994619A (en) * 1996-04-01 1999-11-30 University Of Massachusetts, A Public Institution Of Higher Education Of The Commonwealth Of Massachusetts, As Represented By Its Amherst Campus Production of chimeric bovine or porcine animals using cultured inner cell mass cells
CN1241210A (zh) * 1996-10-11 2000-01-12 得克萨斯农业及机械体系综合大学 产生原生殖细胞和转基因动物物种的方法
WO2003034818A1 (fr) 2001-10-24 2003-05-01 Chugai Seiyaku Kabushiki Kaisha Animal non humain a srgf genetiquement modifie
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WO2009104794A1 (ja) 2008-02-22 2009-08-27 国立大学法人 東京大学 遺伝子改変による致死性表現型を持つ動物の繁殖用ファウンダー動物作製法
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AR100216A1 (es) 2014-04-28 2016-09-21 Recombinetics Inc Edición de genes multiplexados
BR112017018932A2 (pt) 2015-03-03 2018-07-31 Univ Minnesota etv2 e usos do mesmo
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CA2991053A1 (en) 2015-06-30 2017-01-05 Regents Of The University Of Minnesota Humanized skeletal muscle
CN108697067A (zh) 2015-10-27 2018-10-23 明尼苏达大学评议会 嵌合胚胎-辅助的器官制备的组合物和方法
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Also Published As

Publication number Publication date
AU2016288671A1 (en) 2018-02-01
EP3316899A1 (en) 2018-05-09
US20210169054A1 (en) 2021-06-10
BR112017028608A2 (pt) 2018-09-04
CO2018000859A2 (es) 2018-07-10
RU2018103234A (ru) 2019-07-31
EP3316899A4 (en) 2019-04-03
US10897880B2 (en) 2021-01-26
CA2991056A1 (en) 2017-01-05
KR20180021135A (ko) 2018-02-28
IL256544A (en) 2018-02-28
CN108472318A (zh) 2018-08-31
JP2018523999A (ja) 2018-08-30
WO2017004388A1 (en) 2017-01-05
US20180177165A1 (en) 2018-06-28

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