CO2018000859A2 - Musculo cardíaco humanizado - Google Patents

Musculo cardíaco humanizado

Info

Publication number
CO2018000859A2
CO2018000859A2 CONC2018/0000859A CO2018000859A CO2018000859A2 CO 2018000859 A2 CO2018000859 A2 CO 2018000859A2 CO 2018000859 A CO2018000859 A CO 2018000859A CO 2018000859 A2 CO2018000859 A2 CO 2018000859A2
Authority
CO
Colombia
Prior art keywords
human
handii
tbx5
nkx2
combination
Prior art date
Application number
CONC2018/0000859A
Other languages
English (en)
Inventor
Daniel J Garry
Mary G Garry
Naoko Koyano-Nakagawa
Original Assignee
Univ Minnesota
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Minnesota filed Critical Univ Minnesota
Publication of CO2018000859A2 publication Critical patent/CO2018000859A2/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0271—Chimeric vertebrates, e.g. comprising exogenous cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276—Knock-out vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/02—Animal zootechnically ameliorated
    • A01K2267/025—Animal producing cells or organs for transplantation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Animal Husbandry (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Public Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)

Abstract

En este documento se describe un método para producir un animal no humano quimérico que expresa un gen NKX2-5, HANDII, TBX5 o una combinación de los mismos que comprende: a) generar una célula animal no humana nula NKX2-5, HANDII, TBX5 o una combinación de las mismas, en la que ambas copias del gen no humano NKX2-5, gen HANDII, gen TBX5 o una combinación de los mismos porta una mutación que impide la producción de proteína funcional NKX2-5, proteína HANDII, proteína TBX5 o una combinación de las mismas en dicho animal no humano; b) crear una mórula o blastocito nulo no humano NKX2-5, HANDII, TBX5 o una combinación de los mismos mediante transferencia nuclear de células somáticas que comprende fusionar un núcleo de dicha célula nula no humana NKX2-5, HANDII, TBX5 o una combinación de las células animales no humanas nulas de a) en un oocito no humano enucleado y activar dicho oocito para que se divida a fin de formar una mórula o blastocito nulo no humano NKX2-5, HANDII, TBX5 o una combinación de los mismos; c) introducir células madre humanas en las no humanas NKX2-5, HANDII, TBX5 o una combinación de blastocitos no humanos nulos b); y d) implantar dicho blastocito de c) en un animal no humano sustituto pseudopreñado para generar un animal quimérico no humano que expresa NKX2-5, HANDII, TBX5 humano o una combinación de los mismos.
CONC2018/0000859A 2015-06-30 2018-01-29 Musculo cardíaco humanizado CO2018000859A2 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201562187040P 2015-06-30 2015-06-30
PCT/US2016/040431 WO2017004388A1 (en) 2015-06-30 2016-06-30 Humanized heart muscle

Publications (1)

Publication Number Publication Date
CO2018000859A2 true CO2018000859A2 (es) 2018-07-10

Family

ID=57609529

Family Applications (1)

Application Number Title Priority Date Filing Date
CONC2018/0000859A CO2018000859A2 (es) 2015-06-30 2018-01-29 Musculo cardíaco humanizado

Country Status (13)

Country Link
US (2) US10897880B2 (es)
EP (1) EP3316899A4 (es)
JP (1) JP2018523999A (es)
KR (1) KR20180021135A (es)
CN (1) CN108472318A (es)
AU (1) AU2016288671A1 (es)
BR (1) BR112017028608A2 (es)
CA (1) CA2991056A1 (es)
CO (1) CO2018000859A2 (es)
IL (1) IL256544A (es)
MX (1) MX2018000071A (es)
RU (1) RU2018103234A (es)
WO (1) WO2017004388A1 (es)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112017018932A2 (pt) 2015-03-03 2018-07-31 Univ Minnesota etv2 e usos do mesmo
RU2018103093A (ru) 2015-06-30 2019-07-31 Реджентс Оф Зэ Юниверсити Оф Миннесота Гуманизированная скелетная мышца
JP2018523999A (ja) 2015-06-30 2018-08-30 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ ヒト化心筋

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WO1997025412A1 (en) * 1996-01-09 1997-07-17 The Regents Of The University Of California Ungulate embryonic stem-like cells, methods of making and using the cells to produce transgenic ungulates
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BR112017018932A2 (pt) 2015-03-03 2018-07-31 Univ Minnesota etv2 e usos do mesmo
RU2018103093A (ru) 2015-06-30 2019-07-31 Реджентс Оф Зэ Юниверсити Оф Миннесота Гуманизированная скелетная мышца
JP2018523999A (ja) 2015-06-30 2018-08-30 リージェンツ オブ ザ ユニバーシティ オブ ミネソタ ヒト化心筋
CN108697067A (zh) 2015-10-27 2018-10-23 明尼苏达大学评议会 嵌合胚胎-辅助的器官制备的组合物和方法
CN108125943A (zh) 2018-01-17 2018-06-08 云南省第二人民医院 前胡醇当归脂作为制备抗动脉粥样硬化药物的应用

Also Published As

Publication number Publication date
RU2018103234A (ru) 2019-07-31
KR20180021135A (ko) 2018-02-28
BR112017028608A2 (pt) 2018-09-04
CA2991056A1 (en) 2017-01-05
JP2018523999A (ja) 2018-08-30
US20180177165A1 (en) 2018-06-28
US20210169054A1 (en) 2021-06-10
US10897880B2 (en) 2021-01-26
AU2016288671A1 (en) 2018-02-01
EP3316899A1 (en) 2018-05-09
MX2018000071A (es) 2018-03-16
WO2017004388A1 (en) 2017-01-05
IL256544A (en) 2018-02-28
CN108472318A (zh) 2018-08-31
EP3316899A4 (en) 2019-04-03

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