CL2017000465A1 - Generación de plantas haploides - Google Patents

Generación de plantas haploides

Info

Publication number
CL2017000465A1
CL2017000465A1 CL2017000465A CL2017000465A CL2017000465A1 CL 2017000465 A1 CL2017000465 A1 CL 2017000465A1 CL 2017000465 A CL2017000465 A CL 2017000465A CL 2017000465 A CL2017000465 A CL 2017000465A CL 2017000465 A1 CL2017000465 A1 CL 2017000465A1
Authority
CL
Chile
Prior art keywords
plants
haploid
present
transgenic
haploid plant
Prior art date
Application number
CL2017000465A
Other languages
English (en)
Inventor
Milena Ouzunova
Andreas Houben
Raheleh Karimi-Ashiyani
Takayoshi Ishii
Nils Stein
Jochen Kumlehm
Christof Bolduan
Frank Breuer
Monika Kloiber-Maitz
Markus Niessen
Britta Schulz
Silke Wieckhorst
Original Assignee
Kws Saat Se & Co Kgaa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP14182719.6A external-priority patent/EP2989889B8/en
Priority claimed from EP14004389.4A external-priority patent/EP3037540A1/en
Application filed by Kws Saat Se & Co Kgaa filed Critical Kws Saat Se & Co Kgaa
Publication of CL2017000465A1 publication Critical patent/CL2017000465A1/es

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/06Processes for producing mutations, e.g. treatment with chemicals or with radiation
    • A01H1/08Methods for producing changes in chromosome number
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/06Roots
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H5/00Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
    • A01H5/10Seeds
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/20Brassicaceae, e.g. canola, broccoli or rucola
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8287Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for fertility modification, e.g. apomixis
    • C12N15/8289Male sterility
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146Genetically Modified [GMO] plants, e.g. transgenic plants

Abstract

<p>LA PRESENTE INVENCIÓN SE RELACIÓN A PLANTAS NO TRANSGÉNICAS Y TRANSGÉNICAS, PREFERIBLEMENTE PLANTAS DE CULTIVO, QUE COMPRENDE UNA MUTACIÓN QUE CAUSA UNA ALTERACIÓN DE LA SECUENCIA DE AMINOÁCIDO EN EL DOMINIO CATD DEL CENTRÓMERO HISTONA H3 (CENH3), PREFERIBLEMENTE DENTRO DEL BUCLE1O LA Α2-HELICE DEL DOMINIO CATD, QUE TIENE LA ACTIVIDAD BIOLÓGICA DE UN INDUCTOR HAPLOIDE. ADEMÁS, LA PRESENTE INVENCIÓN PROPORCIONA MÉTODOS PARA GENERAR LAS PLANTAS DE LA PRESENTE INVENCIÓN Y PLANTAS HAPLOIDES Y DOBLE HAPLOIDES OBTENIBLES AL CRUZAR LAS PLANTAS DE LA PRESENTE INVENCIÓN CON PLANTAS DE TIPO SILVESTRE COMO TAMBIÉN MÉTODO PARA FACILITAR EL INTERCAMBIO DE CITOPLASMA.</p>
CL2017000465A 2014-08-28 2017-02-27 Generación de plantas haploides CL2017000465A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP14182719.6A EP2989889B8 (en) 2014-08-28 2014-08-28 Generation of haploid plants
EP14004389.4A EP3037540A1 (en) 2014-12-23 2014-12-23 Haploid inducer

Publications (1)

Publication Number Publication Date
CL2017000465A1 true CL2017000465A1 (es) 2017-10-06

Family

ID=54147123

Family Applications (2)

Application Number Title Priority Date Filing Date
CL2017000465A CL2017000465A1 (es) 2014-08-28 2017-02-27 Generación de plantas haploides
CL2023000238A CL2023000238A1 (es) 2014-08-28 2023-01-25 Generación de plantas haploides

Family Applications After (1)

Application Number Title Priority Date Filing Date
CL2023000238A CL2023000238A1 (es) 2014-08-28 2023-01-25 Generación de plantas haploides

Country Status (15)

Country Link
US (2) US10993391B2 (es)
EP (1) EP3186381B1 (es)
CN (1) CN106998665B (es)
AU (1) AU2015309289B2 (es)
BR (1) BR112017003555A2 (es)
CA (1) CA2959566A1 (es)
CL (2) CL2017000465A1 (es)
DK (1) DK3186381T3 (es)
EA (1) EA037899B1 (es)
ES (1) ES2926055T3 (es)
HU (1) HUE059854T2 (es)
MX (1) MX2017002519A (es)
PL (1) PL3186381T3 (es)
UA (1) UA124293C2 (es)
WO (1) WO2016030019A1 (es)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3037540A1 (en) 2014-12-23 2016-06-29 Kws Saat Se Haploid inducer
EP3262177A4 (en) * 2015-02-24 2018-08-08 The Regents of The University of California Haploid induction
WO2018035070A2 (en) * 2016-08-16 2018-02-22 Monsanto Technology Llc Compositions and methods for plant haploid induction
US20200340009A1 (en) * 2018-01-08 2020-10-29 The Regents Of The University Of California Cenh3 deletion mutants
JP6614622B2 (ja) * 2018-04-17 2019-12-04 国立大学法人名古屋大学 植物ゲノム編集方法
CN108624547B (zh) * 2018-05-22 2021-08-13 天津农学院 一种分离单子叶植物保卫细胞的方法
WO2019234132A1 (en) * 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Base editing in polymerase theta deficient plants
WO2019234129A1 (en) 2018-06-05 2019-12-12 KWS SAAT SE & Co. KGaA Haploid induction with modified dna-repair
CN113874501A (zh) * 2018-11-01 2021-12-31 中国科学院遗传与发育生物学研究所 使用碱基编辑器进行靶向诱变
EP3660164A1 (en) 2018-11-30 2020-06-03 KWS SAAT SE & Co. KGaA Screening of germplasm for desired polymorphisms by assays tested on synthetic dna
EP3689135A1 (en) * 2019-01-30 2020-08-05 KWS SAAT SE & Co. KGaA Haploid inducers
CN109997683B (zh) * 2019-04-29 2021-01-01 华南农业大学 一种基于单倍体诱导系的水稻双单倍体育种方法
EP3794939A1 (en) * 2019-09-23 2021-03-24 Leibniz-Institut für Pflanzengenetik und Kulturpflanzenforschung (IPK) Generation of haploids based on mutation of sad2
WO2021239986A1 (en) 2020-05-29 2021-12-02 KWS SAAT SE & Co. KGaA Plant haploid induction
US20230270067A1 (en) * 2020-06-09 2023-08-31 University Of Georgia Research Foundation, Inc. Heterozygous cenh3 monocots and methods of use thereof for haploid induction and simultaneous genome editing
CN114656546B (zh) * 2020-12-03 2023-09-19 中国农业大学 孤雌生殖单倍体诱导基因及其应用
CN113549617B (zh) * 2021-06-03 2023-06-30 南京农业大学 编辑不结球白菜CENH3基因的sgRNA及其CRISPR/Cas9载体和应用
WO2023225469A2 (en) * 2022-05-19 2023-11-23 Syngenta Crop Protection Ag Conferring cytoplasmic male sterility
CN116463348A (zh) * 2023-05-26 2023-07-21 中国农业科学院作物科学研究所 利用CRISPR/Cas9系统编辑玉米ZmCENH3基因的sg RNA及其应用
CN117305326B (zh) * 2023-11-29 2024-02-09 中国农业科学院蔬菜花卉研究所 青花菜BoCENH3基因及其在单倍体诱导中的应用
CN117296710B (zh) * 2023-11-29 2024-03-01 中国农业科学院蔬菜花卉研究所 一种快速创制细胞质雄性不育系的方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2486135B1 (en) 2009-10-06 2020-01-08 The Regents of The University of California Generation of haploid plants and improved plant breeding
WO2014110274A2 (en) 2013-01-09 2014-07-17 Regents Of The University Of California A California Corporation Generation of haploid plants
EP3262177A4 (en) * 2015-02-24 2018-08-08 The Regents of The University of California Haploid induction

Also Published As

Publication number Publication date
US20210259178A1 (en) 2021-08-26
CN106998665A (zh) 2017-08-01
DK3186381T3 (da) 2022-09-12
ES2926055T3 (es) 2022-10-21
PL3186381T3 (pl) 2022-10-31
CL2023000238A1 (es) 2023-07-28
WO2016030019A1 (en) 2016-03-03
US11895960B2 (en) 2024-02-13
EP3186381A1 (en) 2017-07-05
CN106998665B (zh) 2024-02-27
US10993391B2 (en) 2021-05-04
EP3186381B1 (en) 2022-07-06
HUE059854T2 (hu) 2023-01-28
EA201790463A1 (ru) 2017-07-31
EA037899B1 (ru) 2021-06-03
AU2015309289B2 (en) 2021-04-01
UA124293C2 (uk) 2021-08-25
BR112017003555A2 (pt) 2017-12-12
AU2015309289A1 (en) 2017-03-16
US20170280646A1 (en) 2017-10-05
MX2017002519A (es) 2017-09-05
CA2959566A1 (en) 2016-03-03

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