CN113874501A - 使用碱基编辑器进行靶向诱变 - Google Patents

使用碱基编辑器进行靶向诱变 Download PDF

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CN113874501A
CN113874501A CN201980087604.5A CN201980087604A CN113874501A CN 113874501 A CN113874501 A CN 113874501A CN 201980087604 A CN201980087604 A CN 201980087604A CN 113874501 A CN113874501 A CN 113874501A
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梅钰
A·赫梅尔
Z·瓦格齐帕瓦拉
高彩霞
李超
张瑞
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Suzhou Qihe Biotechnology Co ltd
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Abstract

本发明涉及发现性状和产生具有改进表型的细胞系统的新方法。特别地，本发明提供了通过使用具有很少或没有脱靶效应的靶向诱变来开发具有优化农艺表型的植物的方法。靶向诱变是通过引入包含一系列靶向感兴趣核酸序列的向导RNA的碱基编辑复合物或STEME复合物来实现的。本发明还涉及通过本文所述的方法获得的细胞系统，以及包含向导RNA阵列的碱基编辑复合物或STEME复合物用于产生具有重要农艺表型的细胞系统和用于鉴定重要农艺表型的用途。

Description

使用碱基编辑器进行靶向诱变
技术领域
本发明涉及发现性状和产生具有改进表型的细胞系统的新方法。特别地，本发明提供了通过使用具有很少或没有脱靶效应的靶向诱变来开发具有农艺优化表型的植物的方法。靶向诱变是通过引入包含一系列靶向目标核酸序列的向导RNA的碱基编辑复合物来实现的。感兴趣的核酸序列是与农艺学上重要的性状例如抗逆性或高产量相关的基因组序列。本发明还涉及通过本文所述方法获得的细胞系统，以及包含向导RNA阵列的碱基编辑器复合物用于产生具有重要农艺表型的细胞系统和用于鉴定重要农艺表型的用途。
发明背景
几十年来，诱导诱变一直是植物和动物性状发现的遗传变异的宝贵来源。由于突变密度低，诸如化学或辐射诱导的诱变等较旧的技术是费力的(Tadele,Z.(2016).用于剖析植物基因功能的诱变和TILLING。当前基因组学,17(6),499-508)，需要对数千或数百万个人进行筛选以找到感兴趣基因中的一些突变。使用这些方法在单个基因中实现高密度的从头突变实际上是不可能的，而且由于突变随机散布在整个基因组中，使识别感兴趣性状的潜在遗传学变得复杂，因此它们进一步存在问题。
因此，需要改进的诱变技术以加速植物和动物的性状发现。Rodriguez-Leal等人，2017(Rodríguez-Leal、Daniel等人2017年“通过基因组编辑进行作物改良的工程定量性状变异”，细胞，171：470-80.e8.)，描述了CRISPR/Cas9基于调控基因组区域的工具，用于生成缺失突变群体，以探索不同的顺式调控等位基因如何影响数量性状。所描述的方法使用多个gRNA将CRISPR/Cas9复合物靶向所需的区域，在那里它引入了多个双链断裂。结果表明该方法引入了序列重排和多达数千个碱基对的大缺失。
S/G2细胞周期阶段之外的细胞主要通过参与非同源末端连接(NHEJ)修复途径来响应在基因组位点引入双链断裂(DSB)。虽然这些机制通常只是简单地重新连接两端，但在CRISPR/Cas9系统存在的情况下，DSB会反复重新引入，从而更有可能发生插入和缺失(indel)。如果使用多个gRNA在基因组基因座内靶向多个位点，则可以预期插入缺失的积累和基因座的完全破坏，在基因座是编码区时这会导致基因关闭。
为了鉴定可能改善某种性状的突变组合，因此非常需要在不引入DSB的情况下进行靶向诱变，从而导致可追踪的序列修改，而不会完全破坏目标基因座。
碱基编辑器，包括BE(介导C到T转换的碱基编辑器)和ABE(介导A到G转换的腺嘌呤碱基编辑器)，是无需双链切割即可引入直接和可编程突变的强大工具(Komor等人，Nature,2016,533(7603),420-424；Gaudelli等人,Nature,2017,551,464-471)。一般来说，碱基编辑器至少由一个DNA靶向模块和一个使胞苷或腺嘌呤脱氨的催化结构域组成。只要可以将碱基编辑器向导到目标位点，DNA的所有四种转换(A-T到G-C和C-G到T-A)都是可能的。BE和ABE最初是为在哺乳动物细胞系统中工作而开发的，现已优化并应用于植物细胞系统。高效的碱基编辑已在多种植物物种中得到展示(Zong等人,Nature Biotechnology,vol.25,no.5,2017,438-440；Yan等人,Molecular Plant,vol.11,4,2018,631-634；Hua等，Molecular Plant，第11、4、2018卷，627-630)。
碱基编辑器已被用于在基因组序列中引入特定的、定向的替换，在植物和动物中具有已知或预测的表型效应。此外，碱基编辑器已用于靶向哺乳动物细胞基因座内的多个位点(Ma Y等人(2016年)Nature Methods 13,1029-1035，靶向AID介导的诱变(TAM)使哺乳动物细胞的基因组多样化成为可能；和Hess GT等人(2016)Nature Methods 13,1036-1042，在哺乳动物细胞中使用dCas9靶向体细胞超突变进行定向进化)。但到目前为止，它们还没有用于靶向基因内多个位点的定向诱变基因座或几个基因座，以识别植物中的新的或优化的性状。
本发明的一个目的是提供在一个或多个感兴趣的基因组基因座中进行靶向的、密度可调的诱变的手段和方法，其允许鉴定导致改善的表型的突变的特定组合。
本发明的另一个目的是，手段和方法应该特异性靶向某个基因座或某些基因座，而不是在其他基因组区域中引入脱靶突变。此外，不应引入双链断裂以避免插入缺失的积累。
这些方法应该可用于探索基因编码序列和基因调控元件(如启动子、终止子、抑制子和增强子)的广泛应用。
本发明的另一个目的是提供产生具有优化性状的经修饰的细胞系统的手段和方法，其提供改进的农业性能。
发明简述
根据本发明的第一方面，通过一种鉴定细胞系统中农艺重要表型的方法来实现上述目的，包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种的核酸序列的向导RNA阵列或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统；
(d)获得在至少一种感兴趣的核酸序列中包含至少一种修饰的细胞系统；
(e)在获得细胞系统M0群体的条件下培养细胞系统；
(f)筛选细胞系统的M0群体中与至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型；和
(g)鉴定并由此选择细胞系统中农艺学上重要的表型，
其中至少一种碱基编辑复合物的向导RNA阵列包含至少两种向导RNA分子或编码其的序列，靶向至少一种感兴趣的核酸序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
根据另一方面，本发明涉及一种鉴定细胞系统中重要农艺表型的方法，包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸的向导RNA阵列或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统的遗传物质中；
(d)在获得细胞系统M0群体的条件下培养细胞系统；
(e)将细胞系统的M0群体与包含至少一种感兴趣的核酸序列的细胞系统的野生型群体杂交以获得细胞系统的后代群体；
(f)获得在至少一种感兴趣的核酸序列中具有至少一种修饰的细胞系统的后代群体；
(g)针对与至少一种感兴趣的核酸中的至少一种修饰相关的农艺学重要表型筛选细胞系统的后代群体；和
(h)鉴定并选择细胞系统中农艺学上重要的表型，
其中至少一种碱基编辑复合物的向导RNA阵列包含至少两种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
根据又一方面，本发明涉及一种产生具有重要农艺表型的经修饰的细胞系统的方法，该方法包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统；
(d)获得在至少一种感兴趣的核酸序列中包含至少一种修饰的细胞系统；
(e)在获得细胞系统M0群体的条件下培养细胞系统；
(f)筛选细胞系统的M0群体中与至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型；和
(g)从具有重要农艺学表型的M0群体中鉴定并从而选择细胞系统；和
(h)获得具有重要农艺表型的经修饰的细胞系统，
其中至少一种碱基编辑复合物的向导RNA阵列包含至少两种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
根据另一方面，本发明还涉及一种产生具有农艺学重要表型的修饰的细胞系统后代的方法，该方法包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物，或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统的遗传物质中；
(d)在获得细胞系统M0群体的条件下培养细胞系统；
(e)将细胞系统的M0群体与包含至少一种目的核酸序列的细胞系统的野生型群体杂交以获得细胞系统的后代群体；
(f)获得在至少一种感兴趣的核酸序列中具有至少一种修饰的细胞系统的后代群体；
(g)针对与至少一种目的核酸中的至少一种修饰相关的农艺学重要表型筛选细胞系统的后代群体；和
(h)从具有重要农艺表型的后代群体中鉴定并由此选择细胞系统，
(i)获得具有重要农艺表型的修饰的细胞系统的后代，
其中至少一种碱基编辑复合物的向导RNA阵列包含至少两种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
在本发明各个方面的一个实施方案中，至少一种碱基编辑复合物的向导RNA阵列包含至少三种、至少四种、至少五种、至少六种、至少七种、至少八种、至少九、至少十、至少十一、至少十二、至少十三、至少十四、至少十五、至少十六、至少十七、至少十八、至少十九、至少二十或更多种靶向至少一种感兴趣的核酸序列的单独的向导RNA分子。
在本发明的各个方面的一个实施方案中，至少一种STEME复合物的向导RNA阵列包含至少两种、至少三种、至少四种、至少五种、至少六种、至少七种、至少至少八，至少九，至少十，至少十一，至少十二，至少十三，至少十四，至少十五，至少十六，至少十七，至少十八，至少十九，至少二十或更多种靶向至少一种感兴趣的核酸序列的单独的向导RNA分子。
在本发明各个方面的另一个实施方案中，向导RNA分子靶向感兴趣核酸序列的重叠的和/或不同的片段。
在本发明各个方面的又一实施方案中，将至少一种碱基编辑复合物或其组分作为至少一种质粒、至少一种载体或至少一种线性DNA分子的一部分，作为RNA分子和/或作为RNA和/或蛋白质的预组装复合物引入，。
在本发明各个方面的一个实施方案中，将至少一种STEME复合物或其组分作为至少一种质粒、至少一种载体或至少一种线性DNA分子的一部分，作为RNA分子和/或作为RNA和/或蛋白质的预组装复合物引入。
在本发明各个方面的另一个实施方案中，至少一种碱基编辑器复合物或至少一种STEME复合物通过生物或物理方式引入细胞系统，包括转染，转化，包括通过农杆菌属优选根癌农杆菌的转化，病毒载体，基因枪轰击，使用化学试剂的转染，包括聚乙二醇转染，或其任何组合。
在本发明各个方面的另一个实施方案中，至少一个感兴趣的核酸序列是与农艺学上重要的表型相关的内源基因或遗传元件。
在本发明各个方面的另一个实施方案中，上述内源基因选自编码对非生物胁迫包括干旱胁迫、渗透胁迫、热胁迫、冷胁迫、氧化胁迫、重金属胁迫、氮缺乏、磷缺乏、盐胁迫或涝渍的抗性或耐受性，编码除草剂抗性，包括对草甘膦、草铵膦/膦丝菌素、潮霉素、原卟啉原氧化酶(PPO)抑制剂、ALS抑制剂和麦草畏(一种基因)的抗性；编码对生物胁迫的抗性或耐受性，包括病毒抗性基因、真菌抗性基因、细菌抗性基因、昆虫抗性基因；或编码产量相关性状的基因，包括抗倒伏性、开花时间、抗裂性、种子颜色、胚乳成分或营养含量。
在本发明各个方面的又一个实施方案中，上述遗传元件是编码非编码RNA如rRNA、tRNA、miRNA、siRNA、piRNA、snRNA、snoRNA、lncRNA、反义-RNA、核糖开关或核酶的DNA，或调控序列或调控序列的至少一部分，其中调控序列或其部分包含核心启动子序列、近端启动子序列、顺式调控序列、反式调控序列、基因座控制序列、绝缘子序列、沉默子序列、增强子序列、终止子序列和/或它们的任何组合。
在本发明各个方面的另一个实施方案中，至少一种感兴趣的核酸序列中的至少一种修饰是指至少一种碱基编辑复合物或至少一种STEME复合物在感兴趣的核酸序列中诱导至少1，至少2、至少3、至少4、至少5、至少6、至少7、至少8、至少9、至少10、至少11、至少12、至少13、至少14、至少15、至少16、至少17、至少18、至少19、至少20、至少21、至少22、至少23、至少24、至少25或甚至更多个核苷酸交换。
在本发明各个方面的另一个实施方案中，至少一种碱基编辑器复合物的至少一种碱基编辑器组分或至少一种STEME复合物的至少一种STEME组分包含至少一个核酸识别结构结构域和至少一个核酸编辑结构域，其中至少一个核酸识别结构域独立地选自CRISPR-Cas9、CRISPR-Cpf1、CRISPR-CasX、CRISPR-MAD7、CRISPR-Csm1、CRISPR-Cas9切口酶、CRISPR-Cpf1切口酶、CRISPR-CasX切口酶、CRISPR-MAD7切口酶或CRISPR-Csm1切口酶，并且其中至少一个核酸编辑结构域独立地选自胞苷脱氨酶或腺嘌呤脱氨酶或两者，优选地其中至少一个核酸编辑结构域是独立地选自载脂蛋白B mRNA编辑复合物(APOBEC)家族脱氨酶，优选大鼠衍生的APOBEC、活化诱导的胞苷脱氨酶(AID)、ACF1/ASE脱氨酶、ADAT家族d脱氨酶、ADAR2脱氨酶或PmCDA1脱氨酶、TadA衍生的脱氨酶和/或其任何组合、变体或催化活性片段，并且其中至少一种碱基编辑器组分任选地包含至少一种核定位信号，并且其中至少一种碱基编辑器组分任选地包含至少一种接头序列，优选地XTEN接头，并且其中至少一种碱基编辑器组分任选地包含抑制天然存在的DNA或RNA修复的至少一种组分，优选地尿嘧啶DNA糖基化酶抑制剂(UGI)结构域、噬菌体Mu的Gam蛋白结构域或肌苷碱基切除修复结构域的抑制剂。
在本发明各个方面的又一实施例中，上述至少一种碱基编辑器组件，或编码其的序列，或上述至少一种STEME组件，或编码其的序列，被提供为融合分子。
在本发明的各个方面的另一个实施方案中，上述碱基编辑器复合物的组件，或编码其的序列，或上述STEME复合物的组件，或编码其的序列，被提供为单独的分子。
在本发明各个方面的一个实施方案中，细胞系统选自真核生物，其中真核生物是植物、植物的一部分或植物细胞。
在本发明各个方面的另一个实施方案中，上述植物的部分选自叶、茎、根、出芽的胚根、花、花部分、花瓣、果实、花粉、花粉管、花药花丝、胚珠、胚囊、卵细胞、子房、受精卵、胚、合子胚、体细胞胚、顶端分生组织、维管束、周轮、种子、根和插条。本文使用的植物细胞可以是原生质体细胞。
在本发明各个方面的又一个实施方案中，上述植物、植物的一部分或植物细胞是或源自选自以下的植物物种：大麦(Hordeum vulgare)、球茎大麦(Hordeum bulbusom)、双色高粱(Sorghum bicolor)、甘蔗(Saccharum officinarium)、玉米(Zea mays)、小米(Setaria italic)、小粒稻(Oryza minuta)、水稻(Oryza sativa)、澳洲野生稻(Oryzaaustraliensis)、高秆野生稻(Oryza alta)、普通小麦(Triticum aestivum)、黑麦(Secalecereale)、苹果(Malus domestica)、紫短柄草(Brachypodium distich-yon)、海滨大麦(Hordeum marinum)、节节麦(Aegilops tauschii)、Daucus glochidiatus、甜菜(Betavulgaris)、小胡萝卜(Daucus pusillus)、Daucus muricatus、胡萝卜(Daucus carota)、巨桉(Eucalyptus grandis)、美花烟草(Nicotiana sylvestris)、绒毛状烟草(Nicotianatomentosiformis)、烟草(Nicotiana tabacum)、番茄(Solanum lycopersicum)、马铃薯(Solanum tuberosum)、中果咖啡(Coffea canephora)、葡萄(Vitis vinifera)、Erythrante guttata、螺旋狸藻(Genlisea aurea)、黄瓜(Cucumis sativus)、川桑(Morusnotabilis)、Arabidopsis arenosa、深山南芥(Arabidopsis lyrata)、拟南芥(Arabidopsis thaliana)、喜马拉雅鼠耳芥(Crucihimalaya himalaica)、卵叶须弥芥(Crucihimalaya wallichii)、弯曲碎米荠(Cardamine flexuosa)、北美独行菜(Lepidiumvirginicum)、荠菜(Capsella bursa pastoris)、Olmarabidopsis pumila、筷子芥(Arabishirsute)、欧洲油菜(Brassica napus)、甘蓝(Brassica oeleracia)、芜菁(Brassicarapa)、萝卜(Raphanus sativus)、芥菜(Brassica juncea)、黑芥(Brassica nigra)、Erucavesicaria subsp.sativa、甜橙(Citrus sinensis)、麻风树(Jatropha curcas)、毛果杨(Populus trichocarpa)、蒺藜状苜蓿(Medicago truncatula)、山下鹰嘴豆(Cicer yama-shitae)、Cicer bijugum、鹰嘴豆(Cicer arietinum)、网状鹰嘴豆(Cicer reticulatum)、Cicer judaicum、木豆(Cajanus cajanifolius)、蔓草虫豆(Cajanus scarabaeoides)、菜豆(Phaseolus vulgaris)、大豆(Glycine max)、棉属(Gossypium sp.)、紫云英(Astragalus sinicus)、百脉根(Lotus japonicas)、夏堇(Torenia fournieri)、Spinaceaoleracea、菜豆(Phaseolus vulgaris)、蚕豆(Vicia faba)、洋葱(Allium cepa)、葱(Allium fistulosum)、蒜(Allium sativum)、和韭菜(Allium tuberosum)。
在另一方面，本发明还涉及通过根据上述任一方面和实施例的方法获得的修饰的细胞系统。
在又一方面，本发明涉及至少一种碱基编辑器复合物的用途，该复合物包含靶向细胞系统遗传物质中的至少一种感兴趣的核酸序列的向导RNA阵列，用于
(a)产生具有农艺学重要表型的细胞系统，该表型与至少一种感兴趣的核酸序列中的至少一种修饰相关；和/或
(b)鉴定与细胞系统遗传物质中至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型。
在又一方面，本发明涉及至少一种STEME复合物的用途，所述STEME复合物包含靶向细胞系统的遗传物质中的至少一种感兴趣的核酸序列的向导RNA阵列，用于
(a)产生具有农艺学重要表型的细胞系统，该表型与至少一种感兴趣的核酸序列中的至少一种修饰相关；和/或
(b)鉴定与细胞系统遗传物质中至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型。
附图简述
图1：由于蛋白质诱变和调节基序诱变，碱基编辑器向导RNA在编码DNA序列或启动子序列上平铺的示意图，用于表型发现。(A)编码序列中的诱变；(B)编码序列中活性位点的诱变；(C)启动子中的诱变。
图2：胞苷(C)碱基编辑器(BE)的编辑窗口示意图。编辑窗口由矩形框表示。(A)可以在一个gRNA所针对的一个编辑窗口中编辑两个附近的C。(B)一个gRNA(Guide 1)针对一个编辑窗口内的两个C，另一个gRNA(Guide 2)针对另一个编辑窗口中不同位置的一个C。原始间隔区相邻基序(PAM)以黑色表示。
图3：如实施例4所述，使用碱基编辑器通过从头诱变在OsACCase基因上产生新的HR突变。(A)针对M0代中OsACCase基因功能结构域的40个sgRNA位点的核苷酸替换频率。发生W2125C取代的位置2125用箭头标记。与氨基酸区域一致的sgRNA显示在顶部。(B)Haloxyfop处理后T1编辑水稻在OsACCase-W2125位点的表型。携带OsACCase-W2125C和OsACCase-W2125C&R2126K的突变体和野生型在四叶期用氟草醚(48.6g a.i./ha)处理，10天后拍照。比例尺代表2厘米。
图4：通过融合胞苷和腺苷脱氨酶的STEME碱基编辑。(a)STEME-1、STEME-2、STEME-3和STEME-4的架构。缩写：ecTadA7.10：进化的大肠杆菌TadA；aa：氨基酸；XTEN：16aa接头。(b)A3A-PBE和四种STEME结构(n＝3)的C>T编辑频率的比较。(c)PABE-7和四种STEME结构(n＝3)的A>G编辑频率的比较。未经处理的原生质体样品用作对照。值和误差条表示三个独立生物重复的平均值±sem。
图5.STEME-NG在水稻原生质体中进行饱和诱变。(a)STEME-NG的结构。缩写：ecTadA7.10：进化的大肠杆菌TadA；aa：氨基酸。(b)Pfam产生的OsACC蛋白结构结构域的概述。设计具有正向NGD-3'(D＝A、T或G)PAM和反向互补5'-HCN(H＝A、T或C)PAM的sgRNA。BC-N：生物素羧化酶，N-末端结构域；CPSase_L_D2：氨基甲酰磷酸合酶L链，ATP结合结构域；BC-C：生物素羧化酶，C端结构域；BA：需要生物素的酶，附着结构域结合生物素；ACC中心：乙酰辅酶A羧化酶，中心区；CT：羧基转移酶结构域。
图6.来自水稻OsACC(SEQ ID NO:192)和酵母ScACC(SEQ ID NO:193)的CT结构域的序列比对。参与除草剂结合的关键残基用红色表示：水稻OsACC中的Y1912、W2097、W2125和F2128，以及酵母ACC中的Y1738、W1924、W1953和F1956。筛选中发现的突变是：S1866F(T1692)、A1884P(L1710)、P1927F(P1753)和W2125C(W1953)。
定义
在本发明的上下文中，“农艺学上重要的表型”是植物的表型，其表现出一种或多种新的或优化的性状，其提供改进的农业性能，例如，产量、结构、养分分配、光合作用、碳固存、抗病性、胁迫耐受性、除草剂耐受性、激素信号传导和其他性状类别。农艺学上重要的表型可由植物遗传物质的一个或多个编码或调控区中的任何一种或一种或多种突变的组合引起。修饰可能与空间接近度或基因组背景相关，也可能完全无关。因此，农艺学上重要的表型可能表现出一种或多种多基因性状。
本文使用的术语“核酸序列”是指天然或合成来源的单链或双链DNA或RNA。核酸分子或核酸序列在任何长度的特定序列中分别包含至少一个核苷酸或两个或更多个核苷酸，包括寡核苷酸或多核苷酸。核酸序列可以是基因的编码区或调控区或其一部分，或者包含一个或多个任选地包括调控区的基因。
在本发明的上下文中，术语核酸分子的“修饰”或“修饰”是指核酸序列的变化，其导致核酸序列中的至少一个差异将其与原始序列区分开来。特别地，本发明上下文中的修饰是一个或多个核碱基的取代，这不需要待修饰的DNA中的任何双链断裂。
如本文所用，“细胞系统”是指包含待修饰的感兴趣细胞的全部或部分基因组的至少一种元件。细胞系统因此可以是任何体内或体外系统，也包括无细胞系统。细胞系统包含以合适的方式，即以可用于遗传修饰或操作的形式修饰的靶基因组或基因组序列。细胞系统可以选自，例如，原核或真核细胞，包括动物或植物细胞，或者细胞系统可以包含遗传构建体，该构建体包含待修饰的原核或真核细胞的全部或部分基因组以高度针对性的方式。细胞系统可以作为分离的细胞或载体提供，或者细胞系统可以包含在组织、器官、材料或整个生物体中的细胞网络中，在体内或作为体外分离的系统。在这种情况下，细胞系统的“遗传物质”因此可以理解为生物体基因组的全部或部分，该生物体的遗传物质作为整体或部分存在于待修饰的细胞系统中。优选地，本发明上下文中的“细胞系统”是指细胞、生物体或生物体的部分或组织，优选植物或植物系、植物部分或植物器官、分化的和未分化的植物组织、植物细胞、种子及其衍生物和后代。
如本文所用，“碱基编辑器”是指包含至少一种蛋白质或其片段的脱氨酶蛋白质或复合物，所述蛋白质或其片段能够介导靶向碱基修饰，即，感兴趣碱基的转换导致的感兴趣的点突变。优选地，本发明上下文中的至少一个碱基编辑器包含至少一个用于将碱基编辑器靶向核酸序列的特定位点的核酸识别结构域和至少一个在特定目标位点的至少一个核碱基执行转换的核酸编辑结构域。除了核酸识别结构域和核酸编辑结构域之外，碱基编辑器还可以包含其他成分，例如间隔子、定位信号和抑制天然存在的DNA或RNA修复机制的成分，以确保所需的编辑结果。如本文所用，“饱和靶向内源诱变编辑器(STEME)”是指组合胞苷脱氨酶和腺苷脱氨酶的融合脱氨酶蛋白。除了融合脱氨酶外，STEME还可能包含CRISPR切口酶，如nCas9(D10A)和尿嘧啶DNA糖基化酶抑制剂(UGI)(图1a)。优选地，本发明上下文中的至少一个STEME包含至少一个用于将碱基编辑器靶向核酸序列的特定位点的核酸识别结构域和至少一个执行在特定目标位点至少有一个核碱基的转换的核酸编辑结构域。除了核酸识别结构域和核酸编辑结构域之外，STEME还可以包含其他成分，例如间隔区、定位信号和抑制天然存在的DNA或RNA修复机制的成分，以确保所需的编辑结果。
术语“核酸识别结构域”是指碱基编辑器的组成部分，它通过将碱基编辑器定向到预定位置内的目标位点来确保碱基编辑器的位点特异性。核酸识别结构域可以基于CRISPR系统，该系统使用向导RNA(gRNA)或单向导RNA(sgRNA)特异性识别细胞系统的核酸分子内的靶序列，可以是CRISPR RNA(crRNA)和反式激活crRNA(tracrRNA)。
“CRISPR系统”是指包含CRISPR核酸酶的任何天然存在的系统，其已从其天然环境中分离，并且优选已被修饰或组合成感兴趣的重组构建体以适合作为靶向基因组工程的工具。只要原始野生型CRISPR核酸酶提供DNA识别，即结合特性，任何CRISPR核酸酶都可以使用并任选地重新编程或另外突变以适用于根据本发明的各种实施方案。所述DNA识别可以是PAM(原始间隔区相邻基序)依赖性的。可以为特定应用使用和创建具有优化和工程化PAM识别模式的CRISPR核酸酶。PAM识别代码的扩展可适用于将位点特异性效应复合物靶向感兴趣的目标位点，而与基于野生型CRISPR的核酸酶的原始PAM特异性无关。CRISPR核酸酶还包含天然存在的CRISPR效应子序列或编码它们的相应序列的突变体或催化活性片段或融合体。CRISPR核酸酶还可特别指CRISPR切口酶或什至在其天然环境中具有核酸内切功能的CRISPR多肽的核酸酶缺陷变体。
术语“核酸编辑结构域”是指碱基编辑器或STEME的组件，它启动核苷酸转换以产生所需的编辑。核酸编辑结构域的催化功能可以是胞苷脱氨酶功能或腺嘌呤脱氨酶功能，或两者。
碱基编辑器代表“碱基编辑器复合物”的一个组件，它另外包含一系列gRNA。“gRNA阵列”是指两种或更多种gRNA，它们被设计为各自靶向一种特定的核酸序列。
STEME代表“STEME复合物”的一个组成部分，它另外包含一系列gRNA。“gRNA阵列”是指一种gRNA或优选一种或多种gRNA，它们被设计为各自靶向一种特定的核酸序列。
“M0群体”是指在细胞系统中诱变后培养获得的一定数量的细胞系统个体。M0群体在诱变中靶向的遗传物质的核酸序列中表现出多种不同的修饰。相反，“M1群体”是通过杂交M0群体获得的。在本发明的上下文中，诱变后通过培养获得的M0群体优选与野生型细胞系统或野生型群体杂交。
发明详述
本发明提供了一种使用碱基编辑器在单个感兴趣的基因(或少数感兴趣的基因)中引起靶向、超高密度、从头诱变并随后筛选诱变群体以寻找新的或优化的性状的方法。在目标区域以外的区域几乎没有(如果有的话)或没有脱靶效应，并且引入不希望的插入缺失的风险被最小化。该方法允许根据目标序列和所需的多样性微调突变密度。
本文描述的方法可用于其中大量高密度遗传变异在发现新的或优化的性状方面可能有价值的任何情况。在植物中，这可以包括产量、形态、结构、养分分配、光合作用、碳固存、抗病性、胁迫耐受性、除草剂耐受性、激素信号传导、生育力和其他性状类别。
在本发明的第一方面，提供了一种用于鉴定细胞系统中重要农艺表型的方法，包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物，或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣核酸序列的向导RNA阵列，或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统；
(d)获得在至少一种感兴趣的核酸序列中包含至少一种修饰的细胞系统；
(e)在获得细胞系统M0群体的条件下培养细胞系统；
(f)筛选细胞系统的M0群体中与至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型；和
(g)在细胞系统中识别并选择农艺学上重要的表型，
其中所述向导RNA阵列包含靶向至少一种感兴趣的核酸序列的至少两种向导RNA分子或编码其的序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
为了鉴定表现出一种或多种改良的或新的性状的农艺学上重要的表型，可以在步骤(a)中选择感兴趣的核酸序列，其中可以预期序列多样性会产生有用的表型。特别感兴趣的可能是基因或其他基因组元件，其中序列多样性已被证明会影响有价值的表型，但尚未探索所有潜在序列多样性的范围。这适用于农业中的绝大多数重要性状，因为迄今为止不可能在不引入脱靶效应和避免插入/缺失(InDel)形成的情况下进行目标特异性密度可调诱变。
然而，为了发现新的性状或改善性状的新方法，可能还对靶序列感兴趣，已知这些序列对某些表型有影响。
感兴趣的核酸序列可以包括基因编码序列的所有部分、编码非编码RNA的DNA序列，如rRNA、tRNA、miRNA、siRNA、piRNA、snRNA、snoRNA、lncRNA、反义RNA、核糖开关或核酶的DNA，或调控元件，如作为启动子、终止子、增强子或抑制子。这些元件中的任何一个都可以单独或组合作为目标。有利地，本发明的方法还允许鉴定由看似不相关的基因组区域中的突变组合引起的表型，例如。多基因性状。
为了靶向选定的感兴趣的核酸序列，设计了一系列gRNA。gRNA阵列决定了经历高密度诱变的区域。值得注意的是，在编码某种蛋白质的目标核酸中，编码蛋白质活性位点的序列可能会或可能不会被靶向，这取决于所需的结果(图1A和1B)。要诱变的基因的基因靶标和特定特征取决于预期产生感兴趣表型的遗传多样性类型。例如，优选的设计可能是靶向编码植物酶活性位点的序列进行诱变，以便在该酶的活性位点产生结构和化学多样性(图1B)。另一种设计可能针对目标基因的启动子区域(图1C)。许多不同的gRNA阵列可能对单个基因组目标有用，以便优先获得不同的诱变谱。特定的gRNA几乎没有或没有潜在的脱靶效应。然而，即使观察到脱靶效应，这会导致所需表型出现问题，这些突变体也不会在筛选中被选中。
阵列的设计取决于目标区域的大小和所需的突变密度，例如可能因不同的靶基因而异。如果编码序列是目标区域，则重点放在第一个和/或第二个核碱基上。有可能相同的核碱基突变为不同的核碱基。例如，基于胞苷脱氨酶的碱基编辑器主要将C转化为T，但它也可以在较低频率下产生C到A或C到G的副产物。
诱变是在编辑窗口内进行的，即目标区域的一部分，其中核苷酸被编辑。编辑窗口的大小和位置由基本编辑器决定。例如，使用不同的脱氨酶会导致不同的编辑窗口。碱基编辑器还可包含多于一个脱氨酶结构域，其可通过本领域公知的技术彼此连接，从而影响编辑窗口的大小和位置。STEME是这样的脱氨酶融合蛋白，其包含一个以上脱氨酶结构域，优选至少一个胞苷脱氨酶结构域和至少一个腺嘌呤脱氨酶结构域。在编辑窗口之外，通常不会编辑任何碱基。例如，在BE-PLUS系统中，一个dCas9结构域可以招募10个APOBEC结构域，而在CRISPR-X系统中，一个dCas9结构域可以招募4个AID结构域。
如果要编辑的多个位点都在附近，则在编辑窗口内，这可以通过使用单个gRNA来实现(图2A)。如果要编辑的位点彼此相距更远，则可以传递多个gRNA，以便将碱基编辑器定位到不同的位置，并可以在这些不同的位置进行编辑(图2B)。以任何位点或多个位点为目标的自由仅受是否存在合适的PAM以及基本编辑器或STEME的编辑窗口的限制。
在编辑窗口中，所使用的特定碱基编辑器可能有多个目标。在这种情况下，可以编辑所有目标或仅编辑一个或几个。此外，如上所述，相同的核碱基可以突变为不同的核碱基。因此，可以通过本发明的方法创建高度多样性的碱基编辑并发现新的和改进的性状。
将细胞再生或植入整个生物体进行表型筛选可能是合适的。筛选足够大的群体以确保评估所有可能的诱变多样性非常重要。可能的诱变结果的复杂性直接由影响表型的变化的数量决定，它是碱基编辑器或STEME目标密度的函数、碱基编辑器或STEME的特定特征以及会发生突变的碱基转化次数的函数。导致对表型的影响。考虑可能的诱变结果的范围和频率，并筛选足够大的群体是非常重要的。一般来说，目标数量越多，群体规模就应该越大。
此外，群体规模取决于性状及其表型的可能性。一般来说，目标区域的大小和所需的突变密度决定了所需的gRNA数量，这也决定了筛选的人口规模。更大的目标区域和高密度的所需突变需要更多的gRNA和更大的群体规模。
碱基编辑可以产生纯合突变或双等位基因突变。否则可以通过自交获得纯合植物。可以按照Rodriguez-Leal等人，2017年(Rodríguez-Leal，Daniel等人，2017年.'Engineering Quantitative Trait Variation for Crop Improvement by GenomeEditing',Cell,171:470-80.e8.)
有几种策略可用，通过这些策略可以生成用于筛选的诱变群体。在植物中，一种策略是将含有碱基编辑器和向导RNA或STEME和向导RNA的表达盒的单个或多个DNA分子输送到细胞或组织，然后应用再生过程，该过程将产生数百或数千个独特的M0植物。将针对表型筛选该M0植物群体或其后代。这种方法的缺点是，产生如此大量的M0植物所涉及的劳动实际上很困难，在某些情况下，实际上不可能在没有非常有效的DNA传递、选择和再生系统的物种中实现。
这种方法的替代方法是生成少量M0植物，其中包含完整的碱基编辑器和向导RNA表达盒或完整的STEME和向导RNA表达盒。然后将这些植物或其后代与其他植物杂交以产生大量后代，并对后代群体进行表型筛选。尽管由于产生筛选群体需要至少一个额外的世代，因此该方法需要更多时间，但由于与再生大量植物群体相比易于杂交植物以产生大量群体，因此劳动力需求显着降低。
因此，另一方面，本发明提供了一种鉴定细胞系统中重要农艺表型的方法，包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物，或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列，或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统的遗传物质中；
(d)在获得细胞系统M0群体的条件下培养细胞系统；
(e)将细胞系统的M0群体与包含至少一种目的核酸序列的细胞系统的野生型群体杂交以获得细胞系统的后代群体；
(f)获得在至少一种感兴趣的核酸序列中具有至少一种修饰的细胞系统的后代群体；
(g)针对与至少一种目的核酸中的至少一种修饰相关的农艺学重要表型筛选细胞系统的后代群体；和
(h)在细胞系统中鉴定并选择农艺学上重要的表型，
其中所述向导RNA阵列包含靶向至少一种感兴趣的核酸序列的至少两个向导RNA分子或编码其的序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
在上述方法中，通过异交和对来自杂交的基因组的野生型拷贝的碱基编辑作用产生群体。使用这种方法，基本上可以产生用于筛选的任何植物物种的大型突变群体。
本发明还涉及一种产生具有重要农艺表型的改良细胞系统的方法。使用碱基编辑器或STEME在单个感兴趣的基因(或少数感兴趣的基因)中引起靶向诱变，可以产生具有新的或优化的性状的表型，例如提高产量、抗病性、胁迫耐受性、除草剂耐受性和其他性状类别。由于该方法的目标特异性和避免双链断裂，很少(如果有的话)或没有观察到脱靶效应或InDel形成。
根据另一方面，本发明因此提供了一种产生具有重要农艺表型的修饰的细胞系统的方法，该方法包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物，或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列，或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统；
(d)获得在至少一种感兴趣的核酸序列中包含至少一种修饰的细胞系统；
(e)在获得细胞系统M0群体的条件下培养细胞系统；
(f)筛选细胞系统的M0群体中与至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型；和
(g)从具有重要农艺学表型的M0群体中鉴定并从而选择细胞系统；和
(h)获得具有重要农艺表型的改良细胞系统，
其中所述向导RNA阵列包含靶向至少一种感兴趣的核酸序列的至少两种向导RNA分子或编码其的序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
可能已经使用如上所述的鉴定农艺重要表型的方法预先鉴定了农艺重要表型。因此，要靶向的感兴趣的核酸序列可能是已知的，或者可能从其他来源获知，遗传材料中一个或多个核酸序列中的突变对所需的重要农艺表型。
农艺学上重要的表型可能是由基因编码序列、编码非编码RNA的DNA序列或调控元件(例如启动子、终止子或抑制子)的任何部分的突变引起的。因此，这些元素中的任何一个都可以单独或组合作为目标。有利地，本发明的方法还允许通过特异性靶向所有这些区域来产生由远距离基因组区域(例如多基因性状)中的突变组合引起的表型。
在碱基编辑器复合物的情况下，至少两个但可能更多的gRNA的阵列被设计用于靶向选定的感兴趣的核酸序列，在STEME复合物的情况下，至少一个或唯一一个的阵列，但可能更多的gRNA被设计用于靶向选定的感兴趣的核酸序列。如果准确知道需要突变以产生重要的农艺表型，则可以专门设计gRNA以靶向这些位点。如上文在鉴定农艺学重要表型的方法的上下文中已经描述的，技术人员知道使用哪些碱基编辑器来靶向某些核苷酸以及如何调整编辑窗口的大小和位置。如果编码序列是目标区域，则重点放在第一个和/或第二个核碱基上。例如，使用带有胞苷脱氨酶的碱基编辑器，C's是主要导致C到T转化的目标，作为主要产物。然而，如上所述，可能会形成副产物，这可能会或可能不会导致所需的表型。此外，并非编辑窗口内的所有目标核苷酸都可以转换，这也可能会也可能不会导致所需的表型。因此，为了挑选出不提供所需表型的突变体，对M0群体进行表型筛选，并相应地选择突变体。
诱变后，培养细胞系统以获得用于筛选的M0群体。如上文在鉴定农艺学重要表型的方法中已经描述的，需要根据诱变目标调整群体大小以进行筛选。
为了生成用于筛选的诱变群体，可以将包含碱基编辑器和向导RNA表达盒的单个或多个DNA分子递送至细胞或组织，然后可以应用再生过程，从而产生数百或数千个独特的M0植物.将针对表型筛选该M0植物群体或其后代。
正如上面已经提到的，这种方法的缺点是产生如此大量的M0植物所涉及的劳动实际上很困难，在某些情况下，在某些物种中实际上不可能实现。因此，或者，可以生成少量包含全套碱基编辑器/STEME和向导RNA表达盒的M0植物，并将其与其他植物杂交以产生大量后代，并对后代群体进行表型筛选。
因此，另一方面，本发明提供了一种产生具有重要农艺表型的修饰细胞系统后代的方法，该方法包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物，或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列，或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统的遗传物质中；
(d)在获得细胞系统M0群体的条件下培养细胞系统；
(e)将细胞系统的M0群体与包含至少一种目的核酸序列的细胞系统的野生型群体杂交以获得细胞系统的后代群体；
(f)获得在至少一种感兴趣的核酸序列中具有至少一种修饰的细胞系统的后代群体；
(g)针对与至少一种目的核酸中的至少一种修饰相关的农艺学重要表型筛选细胞系统的后代群体；和
(h)从具有重要农艺表型的后代群体中鉴定并由此选择细胞系统，
(i)获得具有重要农艺表型的改良细胞系统的后代，
其中所述向导RNA阵列包含靶向至少一种感兴趣的核酸序列的至少两种向导RNA分子或编码其的序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
使用这种通过将M0群体与野生型群体杂交来产生后代的策略，基本上可以产生用于筛选的任何植物物种的大型突变群体。
在上述本发明的各个方面的一个实施方案中，碱基编辑复合物的向导RNA阵列包含至少三种、至少四种、至少五种、至少六种、至少七种、至少八种、至少三种、至少四种、至少五种、至少六种、至少七种、至少八种、至少九种、至少十种、至少十一种、至少十二种、至少十三种、至少十四种、至少十五种、至少十六种、至少十七种、至少十八种、至少十九种、至少二十种或更多种靶向至少一种感兴趣的核酸序列的单独向导RNA分子。
在上述本发明各个方面的另一个实施方案中，STEME复合物的向导RNA阵列包含至少两种、至少三种、至少四种、至少五种、至少六种、至少七种、至少八、至少九、至少十、至少十一、至少十二、至少十三、至少十四、至少十五、至少十六、至少十七、至少十八、至少十九、至少二十，或多种靶向至少一种感兴趣的核酸序列的单独的向导RNA分子。
在上述本发明各个方面的又一个实施方案中，向导RNA分子靶向感兴趣核酸序列的重叠的和/或不同的片段。
使用的gRNA数量取决于目标区域的大小和所需的突变密度。单个gRNA可以在编辑窗口中引起多个突变，但也可以使用两个或多个gRNA靶向邻近或重叠区域。许多不同的gRNA阵列可能对单个基因组目标有用，以便优先获得不同的诱变谱。阵列的设计取决于诱变的目的，即基因的整个ORF或蛋白质的特定结构域或基因的调节区是否需要诱变(参见实施例2)。
在设计gRNA序列时，多路复用是首选的克隆方法，因为对于个体克隆而言，要执行的构建体和转化的数量以及群体的筛选很容易导致大量的工作和成本。有多种载体系统可用于克隆多重gRNA(参见实施例3)。
在上述本发明各个方面的一个实施方案中，将至少一种碱基编辑器复合物或至少一种STEME复合物或其组分作为至少一种质粒、至少一种载体或至少一种载体的一部分引入一种线性DNA分子，作为RNA分子和/或作为RNA和/或蛋白质的预组装复合物。
在上述本发明各个方面的另一个实施方案中，至少一种碱基编辑器复合物或至少一种STEME复合物通过生物或物理方式引入细胞系统，包括转染，转化，包括通过农杆菌属的转化，优选根癌农杆菌，病毒载体，基因枪轰击，使用化学试剂的转染，包括聚乙二醇转染，或其任何组合。
取决于感兴趣的细胞或细胞系统，可以应用任何合适的递送方法将至少一种碱基编辑器复合物或其组分或至少一种STEME复合物或其组分引入细胞或细胞系统中。因此，本文使用的术语“引入”意味着生物分子或基因构建体(DNA、RNA、单链或双链、蛋白质，包括天然和/或合成成分，或其混合物)进入至少一个细胞或进入感兴趣的隔间，例如细胞核或细胞器，或进入细胞质，其允许转录和/或翻译和/或催化活性和/或结合活性，包括核酸分子与另一个核酸分子的结合，包括DNA或RNA，或蛋白质与至少一个细胞或细胞系统内的靶结构的结合，和/或这样引入的酶的催化活性，任选地在转录和/或翻译之后。
因此，根据本发明的方法，多种递送技术可能适用于将至少一种碱基编辑器复合物或其组分，或至少一种STEME复合物或其组分引入植物细胞或源自植物细胞的细胞系统，递送方法是技术人员已知的，例如通过选择以下直接递送技术，原生质体的聚乙二醇(PEG)处理、程序如电穿孔、显微注射、碳化硅纤维晶须技术、病毒载体介导方法和粒子轰击。
一种常见的生物学方法是用农杆菌属进行转化。几十年来，它已被用于各种不同的植物材料。病毒载体介导的植物转化代表了将遗传物质引入感兴趣的细胞的进一步策略。
值得注意的是，所述用于转化和转染的递送方法可用于同时引入至少一种碱基编辑器复合物的组分。上述递送技术，单独或组合，可用于体内(植物体内)或体外方法。根据本发明的各种实施例，不同的递送技术可以相互组合以引入至少一种碱基编辑器复合物或其组分，或至少一种STEME复合物或其组分。
gRNA阵列可以在一个构建体或多个构建体中提供。gRNA可以从常用的启动子高效表达。
在上述本发明各个方面的一个实施方案中，至少一个感兴趣的核酸序列是与农艺学上重要的表型相关的(一种)内源基因或遗传元件。
编码与农业性能相关的性状的内源基因的修饰可能导致相应性状的改进或优化。另一方面，与这些性状相关的遗传元件(例如调节序列)的修饰也可能对农业性能产生很大影响。还可能需要同时靶向内源性状相关基因和相关调控序列，以鉴定或产生农艺学上重要的表型。
在上述本发明各个方面的一个实施方案中，内源基因选自编码对非生物胁迫包括干旱胁迫、渗透胁迫、热胁迫、冷胁迫。胁迫、氧化胁迫、重金属胁迫、氮缺乏、磷酸盐缺乏、盐胁迫或水涝的抗性或耐受性的基因，编码除草剂抗性，包括对草甘膦、草铵膦/膦酸、潮霉素、原卟啉原氧化酶(PPO)抑制剂、ALS抑制剂和麦草畏(一种编码基因的基因)的抗性的基因，编码对生物胁迫的抗性或耐受性的基因，包括病毒抗性基因、真菌抗性基因、细菌抗性基因、昆虫抗性基因，或编码产量相关性状的基因，包括抗倒伏性、开花时间、抗落粒性、种子颜色，胚乳成分或营养含量。
在上述本发明各个方面的另一个实施方案中，遗传元件是调控序列的至少一部分，其中调控序列包含核心启动子序列、近端启动子序列、顺式调控序列、反式调控序列、基因座控制序列、绝缘子序列、沉默子序列、增强子序列、终止子序列和/或它们的任何组合。
在调节序列中诱导的一种或多种修饰可能导致一种或多种靶基因的表达改变。例如，与未编辑的启动子序列相比，修饰的启动子序列可以显示增加的启动子活性、增加的启动子组织特异性、减少的启动子活性或减少的启动子组织特异性。此外，新的启动子活性、诱导型启动子活性、基因表达的扩展窗口、同一细胞层或其他细胞层中基因表达时间或发育进程的修改，例如，延长花药绒毡层基因表达的时间，DNA结合元件的突变和/或DNA结合元件的缺失或添加可能由修饰引起。
在上述本发明各个方面的一个实施方案中，至少一种碱基编辑器复合物诱导至少1、至少2、至少3、至少4、至少5、至少6、至少7、至少8、至少9、至少10、至少11、至少12、至少13、至少14、至少15、至少16、至少17、至少18、至少19、至少感兴趣的核酸序列中有20、至少21、至少22、至少23、至少24、至少25或甚至更多个核苷酸交换。
根据本发明的方法允许在多个靶核苷酸处进行位点特异性、密度可调的诱变。修改可能与空间接近度或基因组背景相关，也可能完全无关。用根据本发明的方法鉴定或产生的农艺学上重要的表型因此可以表现出一种或多种多基因性状。
在本发明各个方面的另一个实施方案中，至少一个位点特异性碱基编辑器包含至少一个核酸识别结构域和至少一个核酸编辑结构域，并且至少一个STEME包含至少一个核酸识别结构域和至少两个核酸编辑结构域，其中至少一个核酸识别结构域独立地选自任何CRISPR核酸酶的解除武装和切口酶版本，包括但不限于CRISPR-dCas9、CRISPR-dCpf1、CRISPR-dCsm1、CRISPR-dCasX、CRISPR-dCasY、CRISPR-dMAD7、CRISPR-Cas9切口酶、CRISPR-Cpf1切口酶、CRISPR-Csm1切口酶、CRISPR-CasX切口酶、CRISPR-CasY切口酶或CRISPR-MAD7切口酶，或其中至少一种至少两个核酸编辑结构域独立地选自胞苷脱氨酶或腺嘌呤脱氨酶，优选地其中所述至少一个核酸编辑结构域独立地选自载脂蛋白B mRNA-编辑复合物(APOBEC)家族脱氨酶，优选大鼠衍生的APOBEC、活化诱导的胞苷脱氨酶(AID)、ACF1/ASE脱氨酶、ADAT家族脱氨酶、ADAR2脱氨酶或PmCDA1脱氨酶、TadA衍生的脱氨酶和/或其任何组合、变体或催化活性片段，并且其中所述至少一种位点特异性碱基编辑器任选地包含至少一种核定位信号，并且其中所述至少一种碱基编辑器任选地包含至少一种接头序列，优选XTEN接头，并且其中至少一种碱基编辑器任选地包含抑制天然存在的DNA或RNA修复的至少一种成分，优选尿嘧啶DNA糖基化酶抑制剂(UGI)结构域、噬菌体Mu的Gam蛋白结构域或Mu的抑制剂肌苷碱基切除修复结构域。
核酸识别结构域可以基于包含修饰的CRISPR核酸酶的CRISPR系统，该系统将碱基编辑器向导至所需的靶位点但缺乏任何核酸酶功能或优选被修饰以充当切口酶(例如nCas9)。因此，CRISPR核酸酶不会引入双链断裂，而只会在未编辑的链中引入缺口。靶核苷酸的转化由胞苷脱氨酶或腺嘌呤脱氨酶的作用开始。CRISPR核酸识别结构域可以选自不同的生物体，例如，化脓性链球菌或金黄色葡萄球菌。
合适的核酸编辑结构域可包含载脂蛋白B mRNA-编辑复合物(APOBEC)家族脱氨酶，优选大鼠衍生的APOBEC、活化诱导的胞苷脱氨酶(AID)、ACF1/ASE脱氨酶、ADAT家族脱氨酶、ADAR2脱氨酶，或PmCDA1脱氨酶、TadA衍生的脱氨酶和/或其任何组合、变体或催化活性片段。关于适合作为根据本公开内容的碱基编辑器组分的这些和其他脱氨酶的信息可以从WO2015089406A1、WO2017070632A2、WO2017070633A2、WO2018027078A1或WO2015133554A1获得。
关于在碱基编辑的背景下使用噬菌体Mu的Gam蛋白结构域的信息可以在Komor等人中找到，“改进的碱基切除修复抑制和噬菌体Mu Gam蛋白产量C:G-to-T:A碱基编辑器具有更高的效率和产品纯度”，《科学进展》，2017年，Vol.3、8号：eaao4774。
Komor et al.,2016(Komor et al.,Nature,2016,533(7603),420-424)中描述了三种BE版本，即BE1、BE2和BE3，其中BE3显示出靶向C的最高效率T转化，导致人体细胞中高达37％的所需C到T转化。BE3由APOBEC-XTEN-dCas9(A840H)-UGI组成，其中APOBEC1是胞苷脱氨酶，XTEN是16个残基接头，dCas9(A840H)是Cas9的切口酶版本，可在非编辑链上切开，UGI是尿嘧啶DNA糖基化酶抑制剂。在该系统中，BE复合物被sgRNA向导至目标DNA，然后胞嘧啶通过胞嘧啶脱氨作用转化为尿嘧啶。UGI抑制细胞尿嘧啶DNA糖基化酶的功能，该酶催化从DNA中去除尿嘧啶并启动碱基切除修复(BER)。切割未编辑的DNA链有助于将U:G错配解析为所需的U:A和T:A产物。
如上所述，BE在将C转化为T(G到A)方面是有效的，但不能将A转化为G(T到C)。ABE最初由Gaudelli等人于2017年开发(Gaudelli等人，Nature,2017,551,464-471)用于将A-T转换为G-C。进化出一种转移RNA腺苷脱氨酶以对DNA进行操作，该酶催化腺苷脱氨产生肌苷，肌苷被聚合酶读取并复制为G。通过融合进化的腺嘌呤脱氨酶和Cas9模块，Gaudelli等人在2017年(参见上文)中描述的ABE在靶向A到G的转化中显示出约50％的效率。
宗等人(Zong et al.,Nature Biotechnology,vol.25,no.5,2017,438-440)，采用了BE2和BE3(Komor et al.,2016,vide supra)，它们由ratAPOBEC1-Cas9(催化BE2死亡，BE3)-UGI切口酶，密码子优化谷类植物的序列，将它们克隆到玉米Ubiquitin-1基因启动子下，然后将它们应用于水稻、小麦和玉米。他们报告说，使用CRISPR-Cas9切口酶-胞苷脱氨酶融合，原生质体和再生水稻、小麦和玉米植物中C到T的靶向转化显示频率高达43.48％。严等人和华等人两者都报道了采用Gaudelli等人，2017年(见上文)中描述的ABE来在水稻植物中产生靶向AT到GC的突变(Yan等人，Molecular Plant，第11,4,2018卷，631-634；Hua等，Molecular Plant,vol.11,4,2018,627-630)。Yan等人进行了水稻中表达的密码子优化；而华等人使用Gaudelli等人中描述的哺乳动物密码子优化序列，此外还与来自化脓性链球菌和金黄色葡萄球菌的Cas9(D10A)切口酶C末端的强VirD2核定位信号融合。这两项工作都证明了ABE在水稻植物中引入A到G转化的成功应用。
当前基于CRISPR的碱基编辑器在PAM位点和可转换的核苷酸碱基(目前为C->T或A->G)中存在序列限制。由于这种碱基编辑器诱变引起的高遗传多样性增加了可以采样以获得有用表型的可能遗传空间，因此具有较低PAM要求的碱基编辑器通常很有用(以增加感兴趣区域内向导RNA的密度)，在残基转换方面更加灵活(例如，C->T、G或A；A->G、T或C；G->A、C或T；和T->C、A或G)和更大的转换窗口。
优选的碱基编辑器之一是最近开发的A3A-PBE，它由与Cas9切口酶(针对谷类植物的密码子优化)融合的人APOBEC3A(A3A)胞苷脱氨酶组成。这种碱基编辑器的优点是它有一个17个核苷酸的编辑窗口，其活性与序列上下文无关。基本上，A3A碱基编辑器由在Ubi1启动子和CaMV终止子控制下的APOBEC3A-XTEN-nCas9-NLS-UGI-NLS组成(Zong等人，NatureBiotechnology,36,2018,950-953)。该序列是针对谷类植物进行密码子优化的，但可以通过技术人员已知的方式针对其他植物进行优化。与基于大鼠APOBEC1的BE3开发的原始PBE相比，后者具有4-5nt的狭窄编辑窗口，并且在高GC环境下效率低下(Zong等人，NatureBiotechnology，第25卷，第5期，2017年),438-440和Komor等人,2016,同上)A3A碱基编辑器在小麦、水稻和马铃薯中有效地将C转换为T，在所有检查的位点具有17-nt编辑窗口，与序列上下文无关。
具有宽转换窗口的碱基编辑器比具有窄转换窗口的碱基编辑器更具优势，因为它们能够影响每个向导RNA的更多序列空间。为此，最近描述的BE-PLUS系统或类似系统在本发明的上下文中是进一步优选的(Jiang等人，“BE-PLUS：一种具有加宽编辑窗口和增强保真度的新碱基编辑工具”，Cell研究，2018年，第28卷，第8期，855-861)。
此外，发明人设想了这样一种可能性，即使用单个sgRNA的单个蛋白质除了C:G>T:A替换外还可以执行A:T>G:C替换并充当新的饱和靶向内源诱变编辑器(STEME)。图1a)。因此，他们将胞苷脱氨酶与腺苷脱氨酶结合以获得融合脱氨酶。除了融合脱氨酶之外，STEME可能包含例如nCas9(D10A)和尿嘧啶DNA糖基化酶抑制剂(UGI)(图1a)。这新型STEME使用了例如高效胞嘧啶碱基编辑器A3A-PBE，在植物中具有广泛的碱基编辑窗口，植物腺嘌呤碱基编辑器PABE-7包含进化的tRNA腺苷脱氨酶(ecTadA-ecTadA7.10)。为了使用单一蛋白质在同一靶序列中产生C:G>T:A和A:T>G:C取代，发明人将例如APOBEC3A-ecTadA65-ecTadA7.10或ecTadA-ecTadA7.10-APOBEC3A融合到例如nCas9(D10A)的N末端，连同UGI或nCas9(D10A)C末端的两个游离UGI拷贝，产生STEME-1(DNA＝SEQ ID NO:175:APOBEC3A(1…597)-48aa接头(598…741)-ecTadA(742…1239)-32aa接头(1240…1335)-ecTadA7.10(1336…1833)-32aa接头(1834…1929)-nCas9(D10A)-nCas9(D10A)-NLS(6031…6078)-UGI(6097…6345)-NLS(6358…6378)；蛋白质＝SEQ ID NO:176)、STEME-2(DNA＝SEQ ID NO:177:ecTadA(1…501)-32aa接头(502…597)-ecTadA7.10(598…1095)-32aa接头(1096…1191)-APOBEC3A(1192…1785)-16aa接头(1786…1833)-nCas9(D10A)…LS940(5941…5988)-UGI(6007…6255)-NLS(6268…6288)；蛋白质＝SEQ ID NO:178)、STEME-3(DNA＝SEQ ID NO:179:APOBEC3A(1…597)-48aa接头(598…741)-ecTadA(742…1239)-32aa接头(1240…1335)-ecTadA7.10(1195…1833)-32aa接头(1834…1929)-nCas9(D10A)(1930…6030)-NLS(6031…6078)-T2A(6085…6138)-UGI(6139…6387)-NLS(6400…6420)-T2A(6421…6474)-UGI(6421…6474)-UGI(6139…6387)6723)-NLS(6736…6756)；蛋白质＝SEQ ID NO:180)和STEME-4(DNA＝SEQ ID NO:181:ecTadA(1…501)-32aa接头(502…597)-ecTadA7.10(598…1095)-32aa接头(1096)…1191)-APOBEC3A(1192…1785)-16aa接头(1786…1833)-nCas9(D10A)(1834…5934)-NLS(5935…5982)-T2A(5989…6042)-UGI6(291)NLS(6304…6324)-T2A(6325…6378)-UGI(6379…6627)-NLS(6640…6660)；蛋白质＝SEQ ID NO:182。STEME可以针对作物植物进行密码子优化，并由在植物细胞中发挥功能的启动子驱动，如玉米的Ubi-1启动子。C>T基础编辑窗口的范围优选为0.10-60％，其中STEME-1最有效。在A3A-PBE(C1-C17；将PAM远端作为位置1)的主要编辑窗口内，STEME-1在不同基因靶标中显示出平均25.14％的C>T编辑效率。C>T编辑效率比A3A-PBE高1.5倍(平均17.25％)。
STEME-1还显示了四个STEME中最高的A>G碱基编辑效率(0.69-15.50％)和A4到A8的A>G碱基编辑窗口。STEME-1的A>G编辑效率能够为改进的定向进化策略提供所需的多样性。此外，通常在STEME-1的A>G取代的情况下，这伴随着在同一DNA链中同时进行C>T编辑。在任何所需的sgRNA目标上都没有明显的不需要的编辑(<0.05％)。STEMEs的插入缺失频率也与未处理的对照植物细胞相同。STEMEs可以仅使用一种sgRNA来诱导C>T和A>G转换，STEME-1可有效产生同步突变，以增加目标位点突变的多样性。
为了扩大STEME-1或其他STEME的靶向范围，将nCas9(D10A)替换为密码子优化的nCas9-NG(D10A)以产生STEME-NG(DNA＝SEQ ID NO:183:APOBEC3A(1…597)-48aa接头(598…741)-ecTadA(742…1239)-32aa接头(1240…1335)-ecTadA7.10(1336…1833)-32aa接头(1834…1929)(DCas109)1930…6030)-NLS(6031…6078)-UGI(6100…6360)-NLS(6361…6381)；蛋白质＝SEQ ID NO:184)源自STEME 1。STEME-NG具有广泛的编辑能力C>NGPAM序列中的T和A>G，但首选NGD(D＝A、T或G)PAM。与STEME-1(平均C>T 17.89％，A>G3.80％)相比，STEME-NG在规范NGG PAM序列中表现出受损的活性(平均C>T 7.92％，A>G1.84％)。STEME-NG在C1到C17的窗口中编辑胞嘧啶，在A4到A8的窗口中编辑腺嘌呤。此外，STEME-NG在植物细胞中以比pCas9-NG(0.16-13.24％)低得多的频率(<0.10％)产生插入缺失，例如原生质体。总之，STEME-NG的编辑活动取决于Cas9-NG的性质。与NGC PAM相比，它更喜欢NGD PAM。尽管STEME-NG在NGG PAM上的编辑效率平均比STEME-1低2.2倍，但以下数据表明STEME-NG可能扩大C>T和A>G碱基编辑的范围，并可能促进定向进化在植物中的应用。
本发明证明STEME辅助的定向编辑是植物诱变的有效工具。STEME可以产生多种突变，包括碱基替换和框内插入缺失，促进蛋白质功能的分析和农艺性状的发展。同时，通过编辑原生质体获得的高产品纯度和低插入缺失数量表明CRISPR瞬时表达的重要性。STEME系统可用于定向进化，例如需要新的或替代的功能活性的蛋白质编码基因，并且该系统也可应用于植物之外，例如，用于筛选耐药突变体、改变非编码区域的顺式元件和纠正动物的致病性SNV。
在上述本发明的各个方面的一个实施方案中，至少一种碱基编辑器组件，或编码其的序列，或至少一种STEME组件，或编码其的序列，作为融合分子提供.
在上述本发明的各个方面的另一个实施方案中，碱基编辑器复合物的组分，或编码其的序列，或至少一种STEME组分，或编码其的序列，作为单独的分子提供。
至少一种碱基编辑器或至少一种STEME的组分可以作为融合分子存在，或作为通过共价或非共价相互作用中的至少一种缔合或被缔合的单个分子存在，使得在至少有一个碱基编辑器复合物在物理上接近。
融合可以例如提供碱基编辑器的亚细胞定位(例如，用于靶向(例如位点特异性核酸酶)细胞核的核定位信号(NLS)，用于靶向线粒体、叶绿体的线粒体定位信号用于靶向叶绿体等的定位信号。
在上述本发明各个方面的一个实施方案中，细胞系统选自真核生物，其中真核生物是植物、植物的一部分或植物细胞。
在上述本发明各个方面的另一个实施方案中，植物部分选自叶、茎、根、出芽的胚根、花、花部分、花瓣、果实、花粉、花粉管、花药细丝、胚珠、胚囊、卵细胞、子房、受精卵、胚胎、合子胚、体细胞胚、顶端分生组织、维管束、周轮、种子、根和插条。植物细胞可以是原生质体。
在上述本发明各个方面的又一个实施方案中，植物、植物的部分或植物细胞是或源自选自下组的植物物种：大麦(Hordeum vulgare)、球茎大麦(Hordeum bulbusom)、双色高粱(Sorghum bicolor)、甘蔗(Saccharum officinarium)、玉米(Zea mays)、小米(Setaria italic)、小粒稻(Oryza minuta)、水稻(Oryza sativa)、澳洲野生稻(Oryzaaustraliensis)、高秆野生稻(Oryza alta)、普通小麦(Triticum aestivum)、黑麦(Secalecereale)、苹果(Malus domestica)、紫短柄草(Brachypodium distich-yon)、海滨大麦(Hordeum marinum)、节节麦(Aegilops tauschii)、Daucus glochidiatus、甜菜(Betavulgaris)、小胡萝卜(Daucus pusillus)、Daucus muricatus、胡萝卜(Daucus carota)、巨桉(Eucalyptus grandis)、美花烟草(Nicotiana sylvestris)、绒毛状烟草(Nicotianatomentosiformis)、烟草(Nicotiana tabacum)、番茄(Solanum lycopersicum)、马铃薯(Solanum tuberosum)、中果咖啡(Coffea canephora)、葡萄(Vitis vinifera)、Erythrante guttata、螺旋狸藻(Genlisea aurea)、黄瓜(Cucumis sativus)、川桑(Morusnotabilis)、Arabidopsis arenosa、深山南芥(Arabidopsis lyrata)、拟南芥(Arabidopsis thaliana)、喜马拉雅鼠耳芥(Crucihimalaya himalaica)、卵叶须弥芥(Crucihimalaya wallichii)、弯曲碎米荠(Cardamine flexuosa)、北美独行菜(Lepidiumvirginicum)、荠菜(Capsella bursa pastoris)、Olmarabidopsis pumila、筷子芥(Arabishirsute)、欧洲油菜(Brassica napus)、甘蓝(Brassica oeleracia)、芜菁(Brassicarapa)、萝卜(Raphanus sativus)、芥菜(Brassica juncea)、黑芥(Brassica nigra)、Erucavesicaria subsp.sativa、甜橙(Citrus sinensis)、麻风树(Jatropha curcas)、毛果杨(Populus trichocarpa)、蒺藜状苜蓿(Medicago truncatula)、山下鹰嘴豆(Cicer yama-shitae)、Cicer bijugum、鹰嘴豆(Cicer arietinum)、网状鹰嘴豆(Cicer reticulatum)、Cicer judaicum、木豆(Cajanus cajanifolius)、蔓草虫豆(Cajanus scarabaeoides)、菜豆(Phaseolus vulgaris)、大豆(Glycine max)、棉属(Gossypium sp.)、紫云英(Astragalus sinicus)、百脉根(Lotus japonicas)、夏堇(Torenia fournieri)、Spinaceaoleracea、菜豆(Phaseolus vulgaris)、蚕豆(Vicia faba)、洋葱(Allium cepa)、葱(Allium fistulosum)、蒜(Allium sativum)、和韭菜(Allium tuberosum)。
根据另一方面，本发明还涉及通过上述方面和实施例中的任一个的方法获得的改进的细胞系统。
根据又一方面，本发明还涉及至少一种碱基编辑复合物或至少一种STEME复合物的用途，所述STEME复合物包含靶向细胞系统遗传物质中的至少一种感兴趣的核酸序列的向导RNA阵列，其用于
(a)产生具有农艺学重要表型的细胞系统，该表型与至少一种感兴趣的核酸序列中的至少一种修饰相关；和/或
(b)鉴定与细胞系统遗传物质中至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型。
对于包含向导RNA阵列的至少一种碱基编辑器复合物的使用或包含向导RNA阵列的至少一种STEME复合物的使用，在上述各个方面和实施方案的上下文中描述的细节和特征相应地适用.
一个优选的实施方案是至少一种碱基编辑复合物或至少一种STEME复合物在鉴定农艺学重要表型的方法中的用途，所述STEME复合物包含靶向细胞系统遗传物质中的至少一种核酸序列的向导RNA阵列在如以上任何方面和实施例中定义的细胞系统中。
另一个优选的实施方案是至少一种碱基编辑器复合物或至少一种STEME复合物在产生修饰的细胞系统的方法中的用途，所述STEME复合物包含靶向细胞系统遗传物质中的至少一种核酸序列的向导RNA阵列具有农艺学上重要的表型或在产生具有如以上任何方面和实施方案中定义的农艺学上重要的表型的修饰细胞系统的后代的方法中。
在根据本发明的与至少一种碱基编辑复合物相关的用途的一个实施方案中，向导RNA阵列包含至少三种、至少四种、至少五种、至少六种、至少七种、至少八种、至少九种、至少十种、至少十一种、至少十二种、至少十三种、至少十四种、至少十五种、至少十六种、至少十七种、至少十八种、至少十九种、至少二十种或更多种单独的靶向至少一种感兴趣的核酸序列的向导RNA分子；和在与至少一种STEME复合物相关的根据本发明的用途中，向导RNA阵列包含至少两种、至少三种、至少四种、至少五种，在至少六、至少七、至少八、至少九、至少十、至少十一、至少十二、至少十三、至少十四、至少十五、至少十六、至少十七、至少十八、至少十九个、至少二十个或更多种靶向至少一种感兴趣的核酸序列的单独的向导RNA分子。
在根据本发明的用途的另一个实施方案中，向导RNA分子靶向感兴趣的核酸序列的重叠的和/或不同的片段。
在根据本发明的用途的另一个实施方案中，将至少一种碱基编辑器复合物或其组分或至少一种STEME复合物或其组分作为至少一种质粒、至少一种载体、或至少一种线性DNA分子，作为RNA分子和/或作为RNA和/或蛋白质的预组装复合物引入。
在根据本发明的用途的一个实施方案中，至少一种碱基编辑器复合物或至少一种STEME复合物通过生物或物理手段引入细胞系统，包括转染，转化，包括通过农杆菌属的转化，优选地根癌农杆菌，病毒载体，基因枪轰击，使用化学试剂的转染，包括聚乙二醇转染，或其任何组合。
在根据本发明的用途的另一个实施方案中，至少一个感兴趣的核酸序列是与农艺学上重要的表型相关的内源基因或遗传元件。
在根据本发明的用途的另一个实施方案中，上述内源基因选自编码对非生物胁迫包括干旱胁迫、渗透胁迫、热胁迫、冷胁迫氧化胁迫、重金属胁迫、氮缺乏、磷酸盐缺乏、盐胁迫或水涝的抗性或耐受性的基因，编码除草剂抗性的基因，包括对草甘膦、草铵膦/膦酸、潮霉素、原卟啉原氧化酶(PPO)抑制剂、ALS抑制剂和麦草畏(一种编码基因的基因)的抗性，对生物胁迫的抗性或耐受性的基因，包括病毒抗性基因、真菌抗性基因、细菌抗性基因、昆虫抗性基因，或编码产量相关性状的基因，包括抗倒伏性、开花时间、抗落粒性、种子颜色，胚乳成分或营养含量。
在根据本发明的用途的又一个实施方案中，上述遗传元件是调控序列的至少一部分，其中调控序列包含核心启动子序列、近端启动子序列、顺式调控序列中的至少一个、反式调控序列、基因座控制序列、绝缘子序列、沉默子序列、增强子序列、终止子序列和/或它们的任何组合。
在根据本发明的用途的一个实施方案中，至少一种碱基编辑器复合物诱导至少1、至少2、至少3、至少4、至少5、至少6、至少7、至少8、至少9、至少10、至少11、至少12、至少13、至少14、至少15、至少16、至少17、至少18、至少19、至少20、至少21、至少22、至少23、至少24、至少25或甚至更多个在感兴趣的核酸序列中的核苷酸交换。
在根据本发明的用途的另一个实施方案中，至少一种碱基编辑器复合物的至少一种碱基编辑器组分包含至少一个核酸识别结构域和至少一个核酸编辑结构域，并且至少一个STEME包含在至少一个核酸识别结构域和至少两个核酸编辑结构域，其中至少一个核酸识别结构域独立地选自任何CRISPR核酸酶的解除武装和切口酶版本，包括但不限于CRISPR-dCas9、CRISPR-dCpf1、CRISPR-dCsm1、CRISPR-dCasX、CRISPR-dCasY、CRISPR-dMAD7、CRISPR-Cas9切口酶、CRISPR-Cpf1切口酶、CRISPR-Csm1切口酶、CRISPR-CasX切口酶、CRISPR-CasY-切口酶、CRISPR-CasY切口酶和至少一个核酸编辑结构域或至少两个核酸编辑结构域独立地选自胞苷脱氨酶或腺嘌呤脱氨酶，优选其中至少一个核酸编辑结构域in独立地选自载脂蛋白B mRNA-编辑复合物(APOBEC)家族脱氨酶，优选大鼠衍生的APOBEC、活化诱导的胞苷脱氨酶(AID)、ACF1/ASE脱氨酶、ADAT家族脱氨酶、ADAR2脱氨酶，或PmCDA1脱氨酶、TadA衍生的脱氨酶和/或其任何组合、变体或催化活性片段，并且其中至少一种位点特异性碱基编辑器任选地包含至少一种核定位信号，并且其中至少一种碱基编辑器任选地包含至少一种接头序列，优选地XTEN接头，并且其中所述至少一种碱基编辑器任选地包含抑制天然存在的DNA或RNA修复的至少一种成分，优选地尿嘧啶DNA糖基化酶抑制剂(UGI)结构域、Gam噬菌体Mu的蛋白质结构域，或肌苷碱基切除修复结构域的抑制剂。
在根据本发明的用途的另一个实施方案中，将至少一种碱基编辑器组分或编码其的序列、或至少一种STEME组分或编码其的序列作为融合分子提供。
在根据本发明的用途的又一个实施方案中，碱基编辑器复合物的组分，或编码其的序列，或STEME复合物的组分，或编码其的序列，作为单独的分子提供。
在根据本发明的用途的一个实施方案中，细胞系统选自真核生物，其中真核生物是植物、植物的一部分或植物细胞。
在根据本发明的用途的另一个实施方案中，上述植物的部分选自叶、茎、根、出现的胚根、花、花部分、花瓣、果实、花粉、花粉管、花药丝、胚珠、胚囊、卵细胞、子房、受精卵、胚胎、合子胚胎、体细胞胚胎、顶端分生组织、维管束、周轮、种子、根和插条。
在根据本发明的用途的另一个实施方案中，上述植物、植物的部分或植物细胞是或源自选自下组的植物物种：大麦(Hordeum vulgare)、球茎大麦(Hordeum bulbusom)、双色高粱(Sorghum bicolor)、甘蔗(Saccharum officinarium)、玉米(Zea mays)、小米(Setaria italic)、小粒稻(Oryza minuta)、水稻(Oryza sativa)、澳洲野生稻(Oryzaaustraliensis)、高秆野生稻(Oryza alta)、普通小麦(Triticum aestivum)、黑麦(Secalecereale)、苹果(Malus domestica)、紫短柄草(Brachypodium distich-yon)、海滨大麦(Hordeum marinum)、节节麦(Aegilops tauschii)、Daucus glochidiatus、甜菜(Betavulgaris)、小胡萝卜(Daucus pusillus)、Daucus muricatus、胡萝卜(Daucus carota)、巨桉(Eucalyptus grandis)、美花烟草(Nicotiana sylvestris)、绒毛状烟草(Nicotianatomentosiformis)、烟草(Nicotiana tabacum)、番茄(Solanum lycopersicum)、马铃薯(Solanum tuberosum)、中果咖啡(Coffea canephora)、葡萄(Vitis vinifera)、Erythrante guttata、螺旋狸藻(Genlisea aurea)、黄瓜(Cucumis sativus)、川桑(Morusnotabilis)、Arabidopsis arenosa、深山南芥(Arabidopsis lyrata)、拟南芥(Arabidopsis thaliana)、喜马拉雅鼠耳芥(Crucihimalaya himalaica)、卵叶须弥芥(Crucihimalaya wallichii)、弯曲碎米荠(Cardamine flexuosa)、北美独行菜(Lepidiumvirginicum)、荠菜(Capsella bursa pastoris)、Olmarabidopsis pumila、筷子芥(Arabishirsute)、欧洲油菜(Brassica napus)、甘蓝(Brassica oeleracia)、芜菁(Brassicarapa)、萝卜(Raphanus sativus)、芥菜(Brassica juncea)、黑芥(Brassica nigra)、Erucavesicaria subsp.sativa、甜橙(Citrus sinensis)、麻风树(Jatropha curcas)、毛果杨(Populus trichocarpa)、蒺藜状苜蓿(Medicago truncatula)、山下鹰嘴豆(Cicer yama-shitae)、Cicer bijugum、鹰嘴豆(Cicer arietinum)、网状鹰嘴豆(Cicer reticulatum)、Cicer judaicum、木豆(Cajanus cajanifolius)、蔓草虫豆(Cajanus scarabaeoides)、菜豆(Phaseolus vulgaris)、大豆(Glycine max)、棉属(Gossypium sp.)、紫云英(Astragalus sinicus)、百脉根(Lotus japonicas)、夏堇(Torenia fournieri)、Spinaceaoleracea、菜豆(Phaseolus vulgaris)、蚕豆(Vicia faba)、洋葱(Allium cepa)、葱(Allium fistulosum)、蒜(Allium sativum)、和韭菜(Allium tuberosum)。
根据又一方面，本发明还涉及通过上述方法获得的修饰的细胞系统。
根据又一方面，本发明还涉及编码饱和靶向内源诱变编辑器(STEME)的核酸分子。优选地，核酸分子包含根据SEQ ID NO:175、SEQ ID NO:177、SEQ ID NO:179、SEQ ID NO:181或SEQ ID NO:183的核苷酸序列；或与SEQ ID NO:175、SEQ ID NO:177、SEQ ID NO:179、SEQ ID NO:181或SEQ ID NO 183具有至少80％、至少85％、至少90％、至少92％、至少94％、至少95％、至少96％、至少97％、至少98％，或至少99％相同性的核苷酸序列，或编码根据SEQ ID NO:176、SEQ ID NO:178、SEQ ID NO:180、SEQ ID NO:182或SEQ ID NO:184的脱氨酶融合蛋白的核苷酸序列；或编码脱氨酶融合蛋白的核苷酸序列，其氨基酸序列与SEQ IDNO:176、SEQ ID NO:178、SEQ ID NO:180、SEQ ID NO:182或SEQ ID NO:184具有至少80％、至少85％、至少90％、至少92％、至少94％、至少95％、至少96％、至少97％、至少98％或至少99％相同性。
根据又一方面，本发明还涉及编码饱和靶向内源诱变编辑器(STEME)的多肽。优选地，所述多肽编码根据SEQ ID NO：176、SEQ ID NO：178、SEQ ID NO：180、SEQ ID NO：182或SEQ ID NO：184的脱氨酶融合蛋白；或氨基酸序列与SEQ ID NO:176、SEQ ID NO:178、SEQID NO:180、SEQ ID NO:182或SEQ ID NO:184具有至少80％、至少85％、至少90％、至少92％、至少94％、至少95％、至少96％、至少至少97％、至少98％或至少99％相同性的脱氨酶融合蛋白。
根据上述任何方法和用途，此类核酸分子或编码的多肽可用作至少一种STEME复合物中的STEME或STEME组分。
实施例
实施例1：本发明中使用的碱基编辑器
有几种碱基编辑器可用于植物中，在基因组DNA中实现C到T的转换或A到G的转换。优选的碱基编辑器之一是最近开发的A3A-PBE，由与上述Cas9切口酶(针对谷类植物密码子优化)融合的人APOBEC3A(A3A)胞苷脱氨酶组成(Zong等，2018，见上文))。这种碱基编辑器的优点是它有一个17个核苷酸的编辑窗口，其活动与序列上下文无关。也可以使用任何其他可用的碱基编辑器。例如，Cas9结构域可以与任何其他CRISPR结构域互换，包括但不限于Cpf1、xCas9、C2c1、CasX、CasY等；胞苷脱氨酶结构域可以是以下之一，但不限于大鼠APOBEC1、PmCDA1、AID。它也可以是两个组件碱基编辑器，例如基于SunTag的BE-PLUS碱基编辑系统(Jiang等，“BE-PLUS：一种具有加宽编辑窗口和增强保真度的新碱基编辑工具”，Cell Research，2018，第28卷，第8期，855-861)。碱基编辑器的胞苷脱氨酶结构域可以被腺嘌呤脱氨酶取代，这将赋予A到G的转换，例如从ecTadA进化和优化的TadA*结构域。
实施例2：用于靶向诱变的向导RNA设计
要诱变的基因的基因靶标和特定特征取决于预期产生感兴趣表型的遗传多样性类型。碱基编辑器与向导RNA结合的灵活性使得靶向基因组中的任何区域成为可能。然而，优选的设计是1)以编码植物基因活性位点的序列为目标(如果此类信息可用)；2)靶向整个编码序列(如果已知基因功能与有价值的性状相关，但详细的结构或功能位点信息未知)或3)靶向基因调控元件，例如启动子、终止子、抑制子和增强子以微调目标基因的表达模式(图1)。
所选碱基编辑器的编辑窗口与实现一定突变密度所需的gRNA数量直接相关。当靶向基因的编码序列时，仅使用引起错义突变的gRNA，排除仅产生无义或同义突变的gRNA以及引起剪接变化的gRNA。还考虑了脱靶效应的可能性，因此如果可能，只包括几乎没有或没有潜在脱靶的gRNA。
实施例3：诱变和群体筛选的克隆策略
向导RNA可以单独克隆，但最好使用多重克隆。
对于个体克隆，使用以下方法。APOBEC1、不含BsaI的部分nCas9和UGI序列(SEQ IDNO:129)在商业上合成(GenScript，南京，中国)并作为中间载体克隆到pUC57(SEQ ID NO:130)中。nCas9的其他部分(SEQ ID NO:131)从pHUE411中切除(Xing,Hui-Li,et al.2014.'A CRISPR/Cas9 toolkit for Multiple Genome Editor in Plant',BMC Plant Biology,14:327.)与SdaI和MluI，并连接到用相同的两种酶消化的中间载体，产生质粒pUC57-APOBEC1-nCas9-UGI(SEQ ID NO:132)。使用XmaJI和SacI切除全长APOBEC1-nCas9-UGI，然后将其亚克隆到已使用相同的两种酶消化的pHUE411中。所得载体pZRH-PBE(SEQ ID NO：133)用于使用限制酶位点BsaI构建sgRNA表达质粒。
对于多路复用，碱基编辑器部分的克隆可以相同，但多个向导RNA被克隆到单个构建体中。例如，在CRISPR/Cas9系统中，此类构建体可以从金门组装或Gibson获得(Xing等人，2014年；Rodríguez-Leal等人，2017年，见上文)；或使用基于tRNA的多重CRISPR/Cas9载体(Xie,Kabin,et al.2015.'Boosting CRISPR/Cas9多重编辑能力与内源性tRNA处理系统'，美国国家科学院院刊，112：3570-75.；
Figure BDA0003144248320000281
et al.2017.'A MultipurposeToolkit to Enable Advanced Genome Engineering in Plants',The Plant Cell,29:1196-217.)；在CRISPR/Cpf1系统中，这可以通过使用单个定制的CRISPR阵列来实现，因为Cpf1能够处理自己的crRNA(Zetsche,Bernd,et al.2016.'Multiplex gene editing byCRISPR–Cpf1 using a single crRNA阵列'，自然生物技术，35：31)；US 62/616,136)。在使用农杆菌介导的转化时，通过混合含有不同向导RNA阵列的农杆菌培养物，或者在使用基因枪传递时，只需在DNA混合物中添加更多含有不同向导RNA阵列的质粒，可以进一步增加要传递的向导RNA的数量。
有几种策略可以生成用于筛选的诱变群体。在植物中，一种策略(筛选策略I)是将包含碱基编辑器和向导RNA的表达盒的单个或多个DNA分子传递到细胞或组织，然后应用再生过程，该过程将产生数百或数千个独特的M0植物。将针对表型筛选该M0植物群体或其后代(参见实施例4)。另一种首选策略(筛选策略II)是使用多重gRNA生成少量M0植物，其中包含完整的碱基编辑器和向导RNA表达盒，将这些转基因植物与野生型群体进行杂交以产生更大的群体。对杂交基因的野生型拷贝进行编辑(Rodríguez-Leal、Daniel等人，2017年。“通过基因组编辑进行作物改良的工程定量性状变异”，细胞，171：470-80.e8.)。
实施例4：水稻乙酰辅酶A羧化酶(ACCase)基因功能结构域的从头诱变
ACCase是植物脂质生物合成中的关键酶，它使乙酰辅酶A羧化形成丙二酰辅酶A，据报道A1992的突变赋予对quizalofop的抗性(Ostlie,Michael等人，2015年。“突变冬小麦的开发和表征”(Triticum aestivum L.)对除草剂quizalofop具有抗性的种质，Theoretical and Applied Genetics，128：343-51.)。为了在水稻中产生对抑制ACCase的除草剂具有抗性的从头多种突变体，设计了40个靶向ACCase基因功能结构域的sgRNA(Délye、Christophe等人，2005年。'Molecular Bases for Sensitivity to Acetyl-CoenzymeA Carboxylase Inhibitors in Black-Grass'，植物生理学，137：794-806)。将单个sgRNA克隆到pZRH-PBE载体中，并将所得构建体(SEQ ID NO:134-171)分别转化到水稻愈伤组织(var.Zhonghua11)中。40种sgRNA的靶序列列于下表2(SEQ ID NO:1、8、11、15、22、27、32、40、42、47、49、53、59、61、64、68、71,73,76,79,81,83,85,88,90,92,94,99,102,104,107,109,113,115,119,122,125,and 127)由38/40sgRNA向导的碱基编辑器以5.9-80.0％的频率对转基因M0植物进行编辑(图3A和表1)，产生86个独特的错义编辑(表2)。M1植物的除草剂抗性分析，使用ACCase抑制剂，氟草醚，烯禾啶或唑啉草酯，属于三个不同的化学组，显示W2125C和双突变W2125C和R2126K都赋予了对氟草酯的抗性，其抗性是田间推荐的比率48.6g a.i./ha(图3B)。W2125C对应于W2027C，其他三种草中自然发生的HR突变，W2125C和R2126K以前没有报道过(Powles,Stephen B.,et al.2010.'Evolution in Action:PlantsResistant to Herbicides'，Annual Review of Plant Biology,61:317-47)。这些结果表明，碱基编辑介导的从头诱变是在植物中产生新的功能获得性突变的有效工具。有趣的是，W2125C是由间隔序列第11位的G到C颠换而不是G到A转换引起的，后者会产生终止密码子。
表1：针对OsACCase的40个位点的核苷酸替换和插入缺失的频率(％)。
Figure BDA0003144248320000291
Figure BDA0003144248320000301
Figure BDA0003144248320000311
表2：分析靶向OsACCase的38个位点的核苷酸和氨基酸替换。该表中不包括间隔序列之外的核苷酸替换和嵌合突变。靶向胞嘧啶或鸟嘌呤以斜体表示，被PBE取代的核苷酸以粗体表示。
Figure BDA0003144248320000312
Figure BDA0003144248320000321
Figure BDA0003144248320000331
Figure BDA0003144248320000341
Figure BDA0003144248320000351
Figure BDA0003144248320000361
实施例5：水稻二羟酸脱水酶(DHAD)中的靶向诱变
DHAD是植物物种中必不可少且高度保守的酶，可催化β-脱水反应产生异亮氨酸、缬氨酸和亮氨酸的α-酮酸前体。最近，发现了一种针对DHAD的天然产物除草剂(Yan等人，2018年.“具有新作用模式的天然产物除草剂的抗性基因导向发现”，《自然》，559：415-18.)。为了产生对抑制DHAD的除草剂具有抗性的多种突变体，设计了16种sgRNA，以靶向水稻DHAD的整个编码序列(SEQ ID NO:172)。使用实施例3中的方法产生具有碱基编辑器和多重sgRNA表达盒的构建体，并且使用农杆菌介导的转化或基因枪递送将这些构建体转化到水稻愈伤组织中。少数转基因M0植物被再生，并在编辑窗口内对碱基替换进行序列分析。如实施例3中所述，将M0植物与野生型水稻植物异交，并使用DHAD抑制剂天冬氨酸筛选F1和/或F2后代的除草剂抗性。
为了靶向在拟南芥DHAD的结构分析中揭示的活性位点(Yan等人，2018年，见上文)，使用了由腺嘌呤脱氨酶和xCas9结构结构域组成的碱基编辑器。在这种情况下，设计了11个Guide，涵盖了活性位点内和周围的大部分氨基酸残基。使用与上述相同的克隆和筛选策略，还使用DHAD抑制剂天冬氨酸筛选该群体的除草剂抗性。
实施例6：小麦蔗糖合酶(SUS)调控结构域中的靶向诱变
蔗糖合酶催化蔗糖转化为果糖和UDP-葡萄糖，这与淀粉生物合成有关。由于淀粉是小麦干种子中的主要成分，淀粉合成对产量有显着影响。拟南芥蔗糖合酶-1的结构分析表明，N端调控结构域参与多个界面相互作用(Zheng,Yi等人，2011年。“拟南芥蔗糖合酶-1的结构及其功能意义”，Journal of生物化学，286：36108-18。)。基于此，小麦SUS1的调控结构域(专注于参与四聚体磷酸化和界面相互作用的保守氨基酸残基)通过碱基编辑进行诱变，以发现产生优化产量的新等位基因(SEQ ID NO：173)。总共设计了12个sgRNA，用于引入保守氨基酸的多个突变。多重sgRNA表达和筛选策略II用于筛选具有优化产量的突变体。
实施例7：SlCLV3启动子区结构域的靶向诱变
据报道，使用CRISPR/Cas9对SlCLV3启动子进行靶向诱变会产生新的顺式调控等位基因以进行数量变异(Rodríguez-Leal等人，2017，见上文)。此处使用碱基编辑器靶向相同的基因。总共设计了14个sgRNA，靶向编码序列(SEQ ID NO：174)上游2kb的SlCLV3启动子区域，不考虑任何预测的顺式调节元件。碱基编辑器和多重sgRNA表达构建体通过农杆菌介导的转化转化到S.lyc(Gupta、Sarika等人，2016年。“植物再生培养基的改良减少了番茄栽培品种M82稳定转基因系的恢复时间”，植物细胞、组织和器官培养(PCTOC)，127：417-23；Rodríguez-Leal等人，2017年，见上文；和
Figure BDA0003144248320000371
等人，2017，见上文)。再生五到十个转基因M0植物，对编辑窗口内位点的碱基替换进行序列分析。筛选来自M0转基因和野生型植物杂交的F1和/或F2后代的果实大小和细胞室数。
实施例8：
为了使用单一蛋白质在同一靶序列中产生C:G>T:A和A:T>G:C取代，发明人将APOBEC3A-ecTadA65ecTadA7.10或ecTadA-ecTadA7.10-APOBEC3A融合到nCas9(D10A)，连同UGI或nCas9(D10A)C末端的两个游离UGI副本，分别产生STEME-1、STEME-2、STEME-3和STEME-4(图4a)。STEMEs是针对作物进行密码子优化的，由玉米的Ubi-1启动子驱动。为了检查它们对内源基因的碱基编辑活动，设计了六种针对不同水稻基因的sgRNA，并将其克隆到pOsU3-esgRNA中。
每个sgRNA与四个STEME中的每一个一起共转染到水稻原生质体中。A3A-PBE、PABE-7和野生型Cas9用作对照。扩增子深度测序表明，所有四个STEME都有效地产生了C>T和A>G转换(图4b、c)。C>T碱基编辑窗口与A3A-PBE等效，编辑效率范围为0.10-61.61％，其中STEME-1效率最高(图4b)。在A3A-PBE(C1-C17；将PAM远端作为位置1)的主要编辑窗口内，STEME-1在OsAAT、OsACC、OsCDC48和OsDEP1中的C>T编辑效率平均为25.14％比A3A-PBE高1.5倍(平均17.25％)(图4c)。
STEME-1在四个STEME和A4到A8的A>G碱基编辑窗口中也具有最高的A>G碱基编辑效率(0.69-15.50％)。尽管这低于PABE-7(1.74-21.54％)，但STEME-1A>G编辑效率仍处于可接受的阈值内，可为改进的定向进化策略提供所需的多样性(图4c)。此外，在超过99％的A>G被STEME-1取代的情况下，这伴随着同一DNA链中的同时C>T编辑。在任何sgRNA目标上都没有明显的不需要的编辑(<0.05％)。STEMEs的插入缺失频率(0.04-0.63％)也与未处理的对照原生质体(0.04-0.51％)相当，远低于Cas9(6.30-15.61％)。这些结果表明，STEMEs仅使用一种sgRNA即可诱导C>88T和A>G转换，并且STEME-1可有效产生同步突变以增加靶位点突变的多样性。
接下来，为了扩大STEME-1的靶向范围以提高其效用，STEME-1中的nCas9(D10A)被密码子优化的nCas9-NG(D10A)取代以产生STEME-NG(图5a)。它还产生了A3A-PBE-NG(DNA＝SEQ ID NO:185；蛋白质＝SEQ ID NO:186)、PABE7-NG(DNA＝SEQ ID NO:187；蛋白质＝SEQID NO:188)和pCas9-NG(DNA＝SEQ ID NO:189；蛋白质＝SEQ ID NO:190)构建体，通过用密码子优化的nCas9-NG(D10A)或Cas9-NG替换A3A-PBE、PABE-7和pCas9的相应部分.它设计了16个20-nt间隔区，带有来自四个不同水稻基因座的NG PAM。然后将STEME-NG与这16种sgRNA中的每一种一起共转染到水稻原生质体中。已经发现STEME-NG在编辑NG PAM序列中的C>T和A>G方面具有广泛的能力，但首选NGD(D＝A、T或G)PAM。与Cas9-NG24一样，与STEME-1(平均C>T 17.89％，A>G 3.80％)相比，STEME-NG在规范NGG PAM序列中表现出受损的活性(平均C>T 7.92％，A>G 1.84％)。STEME-NG在C1到C17的窗口中编辑胞嘧啶，在A4到A8的窗口中编辑腺嘌呤，这分别与对单个A3A-PBE-NG和PABE7-NG观察到的相同。此外，STEME-NG、A3A-PBE-NG和PABE7-NG在水稻原生质体中以比pCas9-NG(0.16-13.24％)低得多的频率(<0.10％)产生插入缺失。综上所述，这些数据表明STEME-NG、A3A-PBE-NG和PABE7-NG在NGPAM的编辑活动主要取决于Cas9-NG的性质。虽然STEME-NG在NGG PAM上的编辑效率平均比STEME-1低2.2倍，但上述数据表明STEME-NG能够扩展C>T和A>G碱基编辑的范围，促进定向进化在植物中的应用。
实施例9：
为了测试STEME在水稻原生质体中实现饱和从头诱变的能力，以乙酰辅酶A羧化酶(OsACC)为例。ACC是脂质生物合成中的关键酶，其羧基转移酶(CT)结构域是除草剂的目标(图5b)。CT结构域中的氨基酸取代可以赋予草除草剂抗性。已经设计了20种sgRNA，包括11种具有正向NGD-3'PAM的sgRNA和9种具有反向互补5'-HCN(H＝A、T或C)PAM，跨越168bp DNA序列，编码CT结构域的56个氨基酸(图5b)。使用STEME-NG，sgRNAs在编辑窗口中覆盖了90.32％的胞嘧啶、40.43％的腺嘌呤、77.78％的鸟嘌呤和38.89％的胸腺嘧啶，总共对应于编码链碱基的61.31％.这些sgRNA已与STEME-NG一起单独共转染到水稻原生质体中。A3A-PBE-NG和pCas9-NG作为对照。扩增子深度测序显示，STEME-NG将编码链上覆盖碱基中96.43％的Cs转化为Ts，63.16％的As转化为Gs，92.86％的Gs转化为As，以及42.86％的Ts转化为Cs；平均碱基编辑效率分别为11.50％、0.35％、13.33％和0.45％。同时，A3A-PBE-NG将编码链上89.29％的Cs编辑为Ts，将92.86％的Gs编辑为As，未发现A>G或T>C取代。在未处理的对照中未检测到碱基转化。使用STEME-NG由这20种sgRNA诱导的突变多样性比使用A3A-PBE-NG观察到的多约两倍。同时发生的C:G>T:A和A:T>G:C事件贡献了18.4％的观察到的STEME-NG多样性，效率高达2.71％。与上述实验一致，STEME-NG在未处理的插入缺失对照原生质体(<0.02％)中显示出不同的目标集，类似于A3A-PBE-NG(<0.01％)，远低于Cas9-NG(0.32-39.72％))。
我们还分析了STEME-NG在目标56个氨基酸中产生的氨基酸替换。我们发现41个氨基酸被替换(包括沉默突变、错义突变和无义突变)。其中，二十四个、十二个和五个氨基酸分别具有一种、两种和三种氨基酸取代。因此，使用STEME-NG的56个氨基酸和仅20个sgRNA发生了接近饱和的诱变(73.21％)。同样，A3A-PBE-NG突变了33个氨基酸，其中26个、6个、1个分别含有1、2、3种氨基酸取代。这些结果共同表明，STEME-NG可以在水稻编码序列中诱导多种突变类型。因此，它有望成为通过原位饱和从头诱变来定向进化内源基因的有力工具。
实施例10：
作为概念验证，STEMEs已被用于水稻植物中ACC的定向进化。选择编码CT结构域400个aa的1,200-nt区域作为诱变目标。总共设计了200个sgRNA，包括118个正向NGD-3'和82个反向互补5'-HCN PAM sgRNA。STEME-1被选择用于具有NGG-3'或5'-CCN PAM的102个sgRNA，而STEME-NG用于剩余的具有NGW-3'或5'-WCN(W＝A或T)PAM的sgRNA.这些sgRNA在编辑窗口中覆盖了94.61％的Cs、48.26％的As、83.39％的Gs和37.46％的Ts，代表了编码链上所有碱基的63.95％。已将这些sgRNA分别插入到二元载体pH-STEME-1-esgRNA或pH-STEME-NG-esgRNA中。为了有效地进行植物转化和基因分型，将200个sgRNA分为27组(第1-27组)。在每组中，汇集了等量的4到11个sgRNA质粒，覆盖了OsACC中的80-142nt。
为了评估转化覆盖率，将从每组再生幼苗中提取的基因组DNA中的向导RNA序列进行扩增，用于扩增子深度测序。已经发现，每组中72.73％至100％的sgRNAs已经转化到植物中，并且总共92.50％(185/200)的sgRNAs已经成功引入。突变覆盖率以深度测序为特征，在覆盖的768个核苷酸中观察到377个核苷酸替换，涉及168个Cs(73.68％)、23个As(15.03％)、164个Gs(61.65％)和22个Ts(18.18％)。每组的平均编辑效率为13.18％。此外，与原生质体中观察到的统一替换不同，STEMEs诱导C>G/A、G>C/T转换和框内插入缺失，以及经典的C:G>T:A和A:T>G:C基础转换。编辑碱基之间的产物分布为81.86％C>T、13.73％C>G、4.41％C>A、76.63％G>A、19.02％G>C、4.35％G>T、100％A>G、100％T>C；这种稍微改变的分布可能是由于原生质体和植物中碱基切除修复机制的差异。因此，STEME也可用于生成C:G>G:C或C:G>A:T替代以及176到规范编辑，这将增强植物中蛋白质定向进化的多样性。
已经分析了在水稻植株中产生的突变读长的细节。在185种sgRNA诱导的495种突变读长中，76.36％、19.80％、3.64％和0.20％分别涉及1、2、3和4个氨基酸替换。此外，2.83％的突变序列涉及A:T>G:C变化，3.84％涉及同时发生的A:T>G:C和C:G>T:A变化。在靶向的400个氨基酸中，209个(52.25％)被改变，产生沉默、错义和无义突变(图6d)。其中，116、66、19、7和1分别具有1、2、3、4和6种氨基酸取代(图6d)。综上所述，这些数据表明STEME能够产生大量185个突变，作为水稻基因组中内源基因定向进化的基础。
实施例11：
为了鉴定所需的突变体，喷洒了常用的ACC抑制剂氟草醚，以选择第1至27组中的抗除草剂幼苗。三周后，出现了一些外观正常的幼苗，并且明显具有抗除草剂的能力。Sanger测序显示第6组中有10个携带突变：7个为P1927F纯合子，2个为杂合子，其余幼苗为Q1926*/P1927F和P1927F双等位突变体；第20组中的两株幼苗携带突变：一株为W2125C纯合子，另一株为A2123T/W2125C杂合子。已经观察到其他幼苗的氟草醚抗性比上面观察到的稍弱，这表明这些可能代表不同的等位基因。Sanger测序显示，第2组中的两株幼苗是S1866F杂合子，而第3组中的三株幼苗是A1884P杂合子。在所有含有S1866F、P1927F或A2123T替换的植物中，这些是C:G>T:A转换的结果，而C:G>G:C转换是所有观察到的W2125C替换的原因。这与水稻植株STEMEs的扩增子数据一致，表明发生了C:G>G:C颠换。相反，观察到的A1884P取代是由不同的活性引起的；两株植物在A1884P密码子中包含一个C:G>G:C颠换，而第三株植物同时包含C:G>G:C颠换和A:T>G:C转变，表明来自STEME-NG的同时脱氨酶活性。W2125C是除草剂抗性突变，已在禾本科植物中报道过(Powles,SB&Yu,Q.Evolution in action:plant resistance to herbicides.Annu.Rev.Plant.Biol.61,317-347(2010).)，表明STEME对ACC的诱变能够产生已知的突变。重要的是，这些结果还证实了STEME可以产生多个新的突变，例如P1927F、S1866F和A1884P，这些突变以前没有报道过。
实施例12：
已经测试了在并发选择压力下使用STEME进行靶向诱变的策略。基于上述结果，选择第6组(P1927)和第20组(W2125)作为代表性目标，并使用修改后的方案转化水稻，其中在愈伤组织诱导和再生期间施加除草剂选择压力。在目标转化中观察到愈伤组织的旺盛生长，而用对照载体转化的愈伤组织死亡。
选择来自第6组和第20组转化的20株植物用于进一步分析，并且都分别携带预期的P1927F或W2125C突变。携带W2125C的二十个突变体中的三个也包含A2123T突变，其核苷酸变化是由A2123密码子内的腺苷和胞苷脱氨酶活性引起的。此外，我们还对含有P1927F、W2125C、S1866F或A1884P的代表性抗性幼苗的OsACC基因进行了测序，未发现其他突变变化。因此，除了先前描述的突变之外，STEME对OsACC的诱变还可以揭示一系列新的功能性除草剂抗性突变，证明它们在进行定向蛋白质进化方面的潜在价值。
为了评估脱靶效应的可能性，扫描了所有包含多达3-nt错配的相似靶位点的基因组序列，并在各自的突变体中对这些位点进行了测序。从该分析中，仅在其中一个突变体(携带A2123T和A2123T/W2125C的双等位基因突变体)中发现了单个脱靶突变，而在任何其他突变体中均未发现脱靶突变。
质粒构建。
STEME-1、STEME-2、STEME-3和STEME-4的胞苷脱氨酶、腺苷脱氨酶、nCas9(D10A)和UGI部分从A3A-PBE或PABE-7扩增，并组装到pJIT163骨架中一步克隆(ClonExpress II一步克隆试剂盒，Vazyme，南京，中国)。使用TransStart FastPfu DNA聚合酶(TransGenBiotech)进行PCR。包含R1335V/L1111R/D1135V/G1218R/E1219F/A1322R/T1337R取代的Cas9变体nCas9-NG(D10A)是商业合成的(GENEWIZ，苏州，中国)。sgRNA构建体pOsU3-esgRNA之前已有描述(Li,C.等人。使用Cas9-腺苷脱氨酶融合在水稻和小麦中扩展碱基编辑。基因组生物学。19,59(2018))。将退火的寡核苷酸插入BsaI(新英格兰生物实验室)消化的pOsU3-esgRNA。为了构建pH-STEME-1-esgRNA和pH-STEME-NG-esgRNA二元载体，将STEME-1和STEME-NG以及OsU3-esgRNA表达盒克隆到pHUE411骨架中。所有引物组均由北京基因组研究所(BGI)合成。
原生质体转染。
我们使用粳稻品种Nipponbare来制备原生质体。如所述进行原生质体分离和转化(Shan,Q.等人，使用TALENs.Mol.Plant 6,1365-1368(2013)在水稻和Brachypodium中进行快速有效的基因修饰)。通过PEG介导的转染将核酸酶和sgRNA质粒DNA各10μg引入原生质体，通过血细胞计数器测量的平均转化效率为40-55％。转染的原生质体在23℃下孵育，转染后60小时收集它们并提取基因组DNA用于扩增子深度测序。
农杆菌介导的水稻愈伤组织转化。
将每组的二元载体以等摩尔比汇集并通过电穿孔转化到根癌农杆菌AGL1中并用于转化约240个水稻愈伤组织。对粳稻品种中花11的愈伤组织细胞进行农杆菌介导的转化如报道32,33。潮霉素(50μg/ml)用于选择转基因植物。
筛选除草剂耐受性。
将T0再生水稻幼苗转移到水中，在生长室(25℃，16小时光照和8小时黑暗)中生长10天，并喷洒氟草醚(34克活性成分ha-1)。使用加压设备以0.2MPa和450L/ha的喷雾量施用除草剂。三周后，鉴定出存活的幼苗。
在培养基中选择抗氟草醚的幼苗。
转化后，在补充有潮霉素(50μg/ml)的愈伤组织诱导培养基上筛选愈伤组织4周。然后将抗潮霉素愈伤组织转移到添加有氟草醚(0.108mg/L)的愈伤组织诱导培养基中。选择六周后，将新鲜明亮的愈伤组织转移到补充有氟草醚(0.108mg/L)的再生培养基中进行再生。
DNA提取。
原生质体的基因组DNA用DNA-Quick Plant System(Tiangen Biotech,Beijing,China)提取。用CTAB提取再生水稻幼苗基因组DNA，对每组幼苗全部取样。目标位点用特异性引物扩增，扩增子用EasyPure PCR纯化试剂盒(TransGen Biotech，北京，中国)纯化，并用NanoDropTM2000分光光度计(Thermo Fisher Scientific，Waltham，MA，USA)定量。
检测可能的脱靶位点。
使用在线工具Cas-OFFinder(Bae,S.,Park,J.&Kim,J.-S.Cas-OFFinder：一种快速且通用的算法，用于搜索潜在的脱靶位点)预测潜在的脱靶位点Cas9 RNA向导的核酸内切酶。生物信息学30,1473-1475(2014)。如果目标上的sgRNA带有NGG PAM，则使用NGG PAM预测脱靶位点。或者，使用NG PAM预测具有NG PAM的目标sgRNA和脱靶位点。在上面的例子中检查了包含最多3-nt错配的脱靶位点。
序列表
<110> 中国科学院遗传与发育研究所
<120> 使用碱基编辑器进行靶向诱变
<130> KWS0307PCT
<150> 62/754,281
<151> 2018-11-01
<160> 193
<170> PatentIn version 3.5
<210> 1
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> R1 wild type
<400> 1
ccagtgctta ttctagggca tat 23
<210> 2
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> R1 target site silent mutation
<400> 2
ccagtgctta ttctagagca tat 23
<210> 3
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> R1 target site R1891S
<400> 3
ccagtgctta ttctagcgca tat 23
<210> 4
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> Target site R1891T mutation
<400> 4
ccagtgctta ttctacagca tat 23
<210> 5
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> Target site R1891K mutation
<400> 5
ccagtgctta ttctaaagca tat 23
<210> 6
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> target site A1892T mutation
<400> 6
ccagtgctta ttctagaaca tat 23
<210> 7
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site R1891K and A1892T
<400> 7
ccagtgctta ttctaaaaca tat 23
<210> 8
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type target site
<400> 8
ccggtgcata cagcgtcttg acc 23
<210> 9
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site D1925N
<400> 9
ccggtgcata cagcgtctta acc 23
<210> 10
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site D1925H
<400> 10
ccggtgcata cagcgtcttc acc 23
<210> 11
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type target site
<400> 11
tctgcactga acaagcttct tgg 23
<210> 12
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutatetd target site A1935V
<400> 12
tctgtactga acaagcttct tgg 23
<210> 13
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site silent
<400> 13
tctgcattga acaagcttct tgg 23
<210> 14
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site S1934F
<400> 14
tttgcactga acaagcttct tgg 23
<210> 15
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type target site
<400> 15
ccacatgcag ttgggtggtc cca 23
<210> 16
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site G1953N
<400> 16
ccacatgcag ttgggtaatc cca 23
<210> 17
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site G1953D
<400> 17
ccacatgcag ttgggtgatc cca 23
<210> 18
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site G1953S
<400> 18
ccacatgcag ttgggtagtc cca 23
<210> 19
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site G1953A
<400> 19
ccacatgcag ttgggtgctc cca 23
<210> 20
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site G1953H
<400> 20
ccacatgcag ttgggtcatc cca 23
<210> 21
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site G1953T
<400> 21
ccacatgcag ttgggtactc cca 23
<210> 22
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type target site
<400> 22
ccatcttact gtttcagatg acc 23
<210> 23
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site D1969N
<400> 23
ccatcttact gtttcaaatg acc 23
<210> 24
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site D1969H
<400> 24
ccatcttact gtttcacatg acc 23
<210> 25
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site D1969N and D1970N
<400> 25
ccatcttact gtttcaaata acc 23
<210> 26
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site D1969H and D1970N
<400> 26
ccatcttact gtttcacata acc 23
<210> 27
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type target sequence
<400> 27
ccctgctgac cctggtcagc ttg 23
<210> 28
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence D2084N
<400> 28
ccctgctgac cctggtcagc tta 23
<210> 29
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutatetd target sequence D2084H
<400> 29
ccctgctgac cctggtcagc ttc 23
<210> 30
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site G2081D
<400> 30
ccctgctgac cctgatcagc ttg 23
<210> 31
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site D2079N, G2081D
<400> 31
ccctgctaac cctgatcaac ttg 23
<210> 32
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type target sequences
<400> 32
ttcctcgtgc tggacaagtg tgg 23
<210> 33
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequences P2091S
<400> 33
tttctcgtgc tggacaagtg tgg 23
<210> 34
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence P2091F
<400> 34
tttttcgtgc tggacaagtg tgg 23
<210> 35
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site P2091F, R2092C
<400> 35
ttttttgtgc tggacaagtg tgg 23
<210> 36
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site P2091L, R2092C
<400> 36
ttctttgtgc tggacaagtg tgg 23
<210> 37
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site R2092C
<400> 37
ttccttgtgc tggacaagtg tgg 23
<210> 38
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site P2091V
<400> 38
ttgttcgtgc tggacaagtg tgg 23
<210> 39
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site P2091C, R2092C
<400> 39
tttgttgtgc tggacaagtg tgg 23
<210> 40
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type target site
<400> 40
caagactgcg caggcattgc tgg 23
<210> 41
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site T2105I
<400> 41
caagattgcg caggcattgc tgg 23
<210> 42
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site R2126K
<400> 42
cctcgctaac tggagaggct tct 23
<210> 43
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site W2125STOP
<400> 43
cctcgctaac tgaagaggct tct 23
<210> 44
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site W2125C
<400> 44
cctcgctaac tgcagaggct tct 23
<210> 45
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site W2125C, R2126K
<400> 45
cctcgctaac tgcaaaggct tct 23
<210> 46
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site W2125C, G2127N
<400> 46
cctcgctaac tgcagaaact tct 23
<210> 47
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type target site
<400> 47
cgactattgt tgagaacctt agg 23
<210> 48
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site T2145I
<400> 48
cgattattgt tgagaacctt agg 23
<210> 49
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 49
ccatggctgc agagctacga gga 23
<210> 50
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target site R2168Q
<400> 50
ccatggctgc agagctacaa gga 23
<210> 51
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence R2168Q G2169E
<400> 51
ccatggctgc agagctacaa gaa 23
<210> 52
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2166K
<400> 52
ccatggctgc aaagctacaa gga 23
<210> 53
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 53
ccgcattgag tgctatgctg aga 23
<210> 54
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence silent
<400> 54
ccgcattgag tgctatgctg aaa 23
<210> 55
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2189K
<400> 55
ccgcattgag tgctatgcta aga 23
<210> 56
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2189K
<400> 56
ccgcattgag tgctatgcta aaa 23
<210> 57
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2189Q
<400> 57
ccgcattgag tgctatgctc aaa 23
<210> 58
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2189N
<400> 58
ccgcattgag tgctatgcta aca 23
<210> 59
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 59
tatgctgaga ggactgcaaa agg 23
<210> 60
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence A2188V
<400> 60
tatgttgaga ggactgcaaa agg 23
<210> 61
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 61
ccaggattgc atgagtcggc ttg 23
<210> 62
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence M2126I
<400> 62
ccaggattgc ataagtcggc ttg 23
<210> 63
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence M2126I
<400> 63
ccaggattgc atgagtcagc ttg 23
<210> 64
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 64
ggagcttatc ttgctcgact tgg 23
<210> 65
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence A1911V
<400> 65
ggagtttatc ttgctcgact tgg 23
<210> 66
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence L1913F
<400> 66
ggagcttatt ttgctcgact tgg 23
<210> 67
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence L1913V
<400> 67
ggagcttatg ttgctcgact tgg 23
<210> 68
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 68
ccgcaagggt taattgagat caa 23
<210> 69
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence silent
<400> 69
ccgcaagggt taattgaaat caa 23
<210> 70
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2204K
<400> 70
ccgcaagggt taattaaaat caa 23
<210> 71
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 71
tgcttattct agggcatata agg 23
<210> 72
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence S1890F
<400> 72
tgcttatttt agggcatata agg 23
<210> 73
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 73
tttacactta catttgtgac tgg 23
<210> 74
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence T1898I
<400> 74
tttatactta catttgtgac tgg 23
<210> 75
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence L1899F
<400> 75
tttacattta catttgtgac tgg 23
<210> 76
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 76
agctcccaca tgcagttggg tgg 23
<210> 77
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence S1947F
<400> 77
agctttcaca tgcagttggg tgg 23
<210> 78
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence S1947F and H1948Y
<400> 78
agcttttaca tgcagttggg tgg 23
<210> 79
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 79
actgtttcag atgaccttga agg 23
<210> 80
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence S1968L
<400> 80
actgttttag atgaccttga agg 23
<210> 81
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 81
gcgtttctaa tatattgagg tgg 23
<210> 82
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence S1975F
<400> 82
gcgtttttaa tatattgagg tgg 23
<210> 83
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 83
tatgttcctg cctacattgg tgg 23
<210> 84
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence P1985L
<400> 84
tatgttcttg cctacattgg tgg 23
<210> 85
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 85
acttccagta acaacaccgt tgg 23
<210> 86
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence P1993A
<400> 86
acttctagta acaacaccgt tgg 23
<210> 87
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence P1993L
<400> 87
acttttagta acaacaccgt tgg 23
<210> 88
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 88
aacaacaccg ttggacccac cgg 23
<210> 89
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence T1995N
<400> 89
aacaataccg ttggacccac cgg 23
<210> 90
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 90
gaactcgtgt gatcctcgag cgg 23
<210> 91
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence S2012L
<400> 91
gaacttgtgt gatcctcgag cgg 23
<210> 92
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 92
gttactggca gagcaaagct tgg 23
<210> 93
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence T2052I
<400> 93
gttattggca gagcaaagct tgg 23
<210> 94
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 94
caaactatcc ctgctgaccc tgg 23
<210> 95
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> muatated target sequence T2075I
<400> 95
caaactattc ctgctgaccc tgg 23
<210> 96
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence P2076I
<400> 96
caaactattc ctgctgaccc tgg 23
<210> 97
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence P2077R
<400> 97
caaactatcc ttgctgaccc tgg 23
<210> 98
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence T2075I and silent
<400> 98
caaattattc ctgctgaccc tgg 23
<210> 99
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 99
atggctgcag agctacgagg agg 23
<210> 100
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence A2064V
<400> 100
atggttgcag agctacgagg agg 23
<210> 101
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence A2064G
<400> 101
atgggtgcag agctacgagg agg 23
<210> 102
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 102
gactgcaaaa ggcaatgttc tgg 23
<210> 103
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence A2192V
<400> 103
gactgtaaaa ggcaatgttc tgg 23
<210> 104
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 104
cccagaccgc attgagtgct atg 23
<210> 105
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence C2185Y
<400> 105
cccagaccgc attgagtact atg 23
<210> 106
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence silent
<400> 106
cccagaccgc attgaatgct atg 23
<210> 107
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 107
cctttgtcta cattcccatg gct 23
<210> 108
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence M2063I
<400> 108
cctttgtcta cattcccatg act 23
<210> 109
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 109
ccagtgggtg tgatagctgt gga 23
<210> 110
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2068K
<400> 110
ccagtgggtg tgatagctgt gaa 23
<210> 111
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2069K
<400> 111
ccagtgggta tgatagctgt gaa 23
<210> 112
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence A2068K, V2067M, silent
<400> 112
ccagtgggta tgatagctat gaa 23
<210> 113
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 113
ccaagggaaa tggttaggtg gta 23
<210> 114
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence G2031N
<400> 114
ccaagggaaa tggttaaatg gta 23
<210> 115
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 115
cctcgagcgg ctatccgtgg tgt 23
<210> 116
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence G2021N
<400> 116
cctcgagcgg ctatccgtaa tgt 23
<210> 117
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence G2021S
<400> 117
cctcgagcgg ctatccgtag tgt 23
<210> 118
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence G2021N, R2020Q
<400> 118
cctcgagcgg ctatccataa tgt 23
<210> 119
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> CCTGAGAACTCGTGTGATCCTCG
<400> 119
cctgagaact cgtgtgatcc tcg 23
<210> 120
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence D2014N
<400> 120
cctgagaact cgtgtaatcc tcg 23
<210> 121
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence C2013Y, D2014N
<400> 121
cctgagaact cgtataatcc tcg 23
<210> 122
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 122
cctgttgcat acattcctga gaa 23
<210> 123
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2010K
<400> 123
cctgttgcat acattcctaa gaa 23
<210> 124
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence E2010K
<400> 124
cctgttgcat acattcctaa aaa 23
<210> 125
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 125
ccgttggacc caccggacag acc 23
<210> 126
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence D2002N
<400> 126
ccgttggacc caccgaacag acc 23
<210> 127
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> wild type
<400> 127
cctattattc ttacaggcta ttc 23
<210> 128
<211> 23
<212> DNA
<213> Artificial Sequence
<220>
<223> mutated target sequence G1932D
<400> 128
cctattattc ttacagacta ttc 23
<210> 129
<211> 2521
<212> DNA
<213> Artificial Sequence
<220>
<223> APOBEC1, partial nCas9 and UGI
<400> 129
cctaggatgc cgaagaagaa gcgcaaggtg tccagcgaga cgggcccagt ggctgtcgac 60
ccaacgctgc gcaggcgcat cgagccgcac gagttcgagg tcttcttcga ccccagggag 120
ctgcgcaagg agacgtgcct cctgtacgag atcaactggg gcggcaggca ctccatctgg 180
aggcacacca gccagaacac gaacaagcac gtggaggtca acttcatcga gaagttcacc 240
acggagaggt acttctgccc gaacacccgc tgctccatca cgtggttcct gtcctggagc 300
ccctgcggcg agtgctccag ggcgatcacc gagttcctca gccgctaccc gcacgtgacg 360
ctgttcatct acatcgctag gctctaccac cacgctgacc ccaggaacag gcagggcctc 420
cgcgacctga tctccagcgg cgtgaccatc cagatcatga cggagcagga gtccggctac 480
tgctggagga acttcgtcaa ctactcccca agcaacgagg ctcactggcc gaggtaccca 540
cacctctggg tgcgcctcta cgtgctcgag ctgtactgca tcatcctcgg cctgccgccc 600
tgcctcaaca tcctgaggcg caagcagccc cagctgacct tcttcacgat cgccctccag 660
agctgccact accagaggct cccaccacac atcctgtggg cgaccggcct caagtccggc 720
agcgagacgc caggcacgtc cgagagcgct acgccagagc tgaaggacaa gaagtactcg 780
atcggcctcg ccattgggac taactctgtt ggctgggccg tgatcaccga cgagtacaag 840
gtgccctcaa agaagttcaa ggtcctgggc aacaccgatc ggcattccat caagaagaat 900
ctcattggcg ctctcctgtt cgacagcggc gagacggctg aggctacgcg gctcaagcgc 960
accgcccgca ggcggtacac gcgcaggaag aatcgcatct gctacctgca ggaagacgcg 1020
tacctgaacg cggtggtcgg cacagctctg atcaagaagt acccaaagct cgagagcgag 1080
ttcgtgtacg gggactacaa ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag 1140
gagattggca aggctaccgc caagtacttc ttctactcta acattatgaa tttcttcaag 1200
acagagatca ctctggccaa tggcgagatc cggaagcgcc ccctcatcga gacgaacggc 1260
gagacggggg agatcgtgtg ggacaagggc agggatttcg cgaccgtcag gaaggttctc 1320
tccatgccac aagtgaatat cgtcaagaag acagaggtcc agactggcgg gttctctaag 1380
gagtcaattc tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa ggactgggat 1440
ccgaagaagt acggcgggtt cgacagcccc actgtggcct actcggtcct ggttgtggcg 1500
aaggttgaga agggcaagtc caagaagctc aagagcgtga aggagctgct ggggatcacg 1560
attatggagc gctccagctt cgagaagaac ccgatcgatt tcctggaggc gaagggctac 1620
aaggaggtga agaaggacct gatcattaag ctccccaagt actcactctt cgagctggag 1680
aacggcagga agcggatgct ggcttccgct ggcgagctgc agaaggggaa cgagctggct 1740
ctgccgtcca agtatgtgaa cttcctctac ctggcctccc actacgagaa gctcaagggc 1800
agccccgagg acaacgagca gaagcagctg ttcgtcgagc agcacaagca ttacctcgac 1860
gagatcattg agcagatttc cgagttctcc aagcgcgtga tcctggccga cgcgaatctg 1920
gataaggtcc tctccgcgta caacaagcac cgcgacaagc caatcaggga gcaggctgag 1980
aatatcattc atctcttcac cctgacgaac ctcggcgccc ctgctgcttt caagtacttc 2040
gacacaacta tcgatcgcaa gaggtacaca agcactaagg aggtcctgga cgcgaccctc 2100
atccaccagt cgattaccgg cctctacgag acgcgcatcg acctgtctca gctcgggggc 2160
gacaagcggc cagcggcgac gaagaaggcg gggcaggcga agaagaagaa gacccgcgac 2220
tccggcggca gcacgaacct ctccgacatc atcgagaagg agacgggcaa gcagctcgtg 2280
atccaggaga gcatcctcat gctgccggag gaggtggagg aggtcatcgg caacaagccc 2340
gagtccgaca tcctcgtgca caccgcctac gacgagtcca cggacgagaa cgtcatgctc 2400
ctgacgagcg acgctccaga gtacaagcca tgggctctcg tgatccagga cagcaacggc 2460
gagaacaaga tcaagatgct gtccggcggc tccccgaaga agaagcgcaa ggtctgagct 2520
c 2521
<210> 130
<211> 1835
<212> DNA
<213> Artificial Sequence
<220>
<223> pUC57 vector
<400> 130
ccaatgatat cggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 60
aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 120
gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 180
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 240
cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 300
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 360
gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 420
cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 480
agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg 540
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 600
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 660
gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 720
cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 780
attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 840
accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 900
ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 960
gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 1020
agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 1080
ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 1140
ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 1200
gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 1260
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 1320
tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 1380
tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 1440
cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca 1500
tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 1560
gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 1620
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 1680
ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 1740
attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 1800
cgcgcacatt tccccgaaaa gtgccacctg acgtc 1835
<210> 131
<211> 2720
<212> DNA
<213> Artificial Sequence
<220>
<223> partial nCas9
<400> 131
cctgcaggag attttctcca acgagatggc gaaggttgac gattctttct tccacaggct 60
ggaggagtca ttcctcgtgg aggaggataa gaagcacgag cggcatccaa tcttcggcaa 120
cattgtcgac gaggttgcct accacgagaa gtaccctacg atctaccatc tgcggaagaa 180
gctcgtggac tccacagata aggcggacct ccgcctgatc tacctcgctc tggcccacat 240
gattaagttc aggggccatt tcctgatcga gggggatctc aacccggaca atagcgatgt 300
tgacaagctg ttcatccagc tcgtgcagac gtacaaccag ctcttcgagg agaaccccat 360
taatgcgtca ggcgtcgacg cgaaggctat cctgtccgct aggctctcga agtctcggcg 420
cctcgagaac ctgatcgccc agctgccggg cgagaagaag aacggcctgt tcgggaatct 480
cattgcgctc agcctggggc tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga 540
cgccaagctg cagctctcca aggacacata cgacgatgac ctggataacc tcctggccca 600
gatcggcgat cagtacgcgg acctgttcct cgctgccaag aatctgtcgg acgccatcct 660
cctgtctgat attctcaggg tgaacaccga gattacgaag gctccgctct cagcctccat 720
gatcaagcgc tacgacgagc accatcagga tctgaccctc ctgaaggcgc tggtcaggca 780
gcagctcccc gagaagtaca aggagatctt cttcgatcag tcgaagaacg gctacgctgg 840
gtacattgac ggcggggcct ctcaggagga gttctacaag ttcatcaagc cgattctgga 900
gaagatggac ggcacggagg agctgctggt gaagctcaat cgcgaggacc tcctgaggaa 960
gcagcggaca ttcgataacg gcagcatccc acaccagatt catctcgggg agctgcacgc 1020
tatcctgagg aggcaggagg acttctaccc tttcctcaag gataaccgcg agaagatcga 1080
gaagattctg actttcagga tcccgtacta cgtcggccca ctcgctaggg gcaactcccg 1140
cttcgcttgg atgacccgca agtcagagga gacgatcacg ccgtggaact tcgaggaggt 1200
ggtcgacaag ggcgctagcg ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa 1260
cctgccaaat gagaaggtgc tccctaagca ctcgctcctg tacgagtact tcacagtcta 1320
caacgagctg actaaggtga agtatgtgac cgagggcatg aggaagccgg ctttcctgtc 1380
tggggagcag aagaaggcca tcgtggacct cctgttcaag accaaccgga aggtcacggt 1440
taagcagctc aaggaggact acttcaagaa gattgagtgc ttcgattcgg tcgagatctc 1500
tggcgttgag gaccgcttca acgcctccct ggggacctac cacgatctcc tgaagatcat 1560
taaggataag gacttcctgg acaacgagga gaatgaggat atcctcgagg acattgtgct 1620
gacactcact ctgttcgagg accgggagat gatcgaggag cgcctgaaga cttacgccca 1680
tctcttcgat gacaaggtca tgaagcagct caagaggagg aggtacaccg gctgggggag 1740
gctgagcagg aagctcatca acggcattcg ggacaagcag tccgggaaga cgatcctcga 1800
cttcctgaag agcgatggct tcgcgaaccg caatttcatg cagctgattc acgatgacag 1860
cctcacattc aaggaggata tccagaaggc tcaggtgagc ggccaggggg actcgctgca 1920
cgagcatatc gcgaacctcg ctggctcgcc agctatcaag aaggggattc tgcagaccgt 1980
gaaggttgtg gacgagctgg tgaaggtcat gggcaggcac aagcctgaga acatcgtcat 2040
tgagatggcc cgggagaatc agaccacgca gaagggccag aagaactcac gcgagaggat 2100
gaagaggatc gaggagggca ttaaggagct ggggtcccag atcctcaagg agcacccggt 2160
ggagaacacg cagctgcaga atgagaagct ctacctgtac tacctccaga atggccgcga 2220
tatgtatgtg gaccaggagc tggatattaa caggctcagc gattacgacg tcgatcatat 2280
cgttccacag tcattcctga aggatgactc cattgacaac aaggtcctca ccaggtcgga 2340
caagaaccgg ggcaagtctg ataatgttcc ttcagaggag gtcgttaaga agatgaagaa 2400
ctactggcgc cagctcctga atgccaagct gatcacgcag cggaagttcg ataacctcac 2460
aaaggctgag aggggcgggc tctctgagct ggacaaggcg ggcttcatca agaggcagct 2520
ggtcgagaca cggcagatca ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac 2580
taagtacgat gagaatgaca agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa 2640
gctcgtctcc gacttcagga aggatttcca gttctacaag gttcgggaga tcaacaatta 2700
ccaccatgcc catgacgcgt 2720
<210> 132
<211> 7059
<212> DNA
<213> Artificial Sequence
<220>
<223> pUC57-APOBEC1-nCas9-UGI vector
<400> 132
ccaatgatcc taggatgccg aagaagaagc gcaaggtgtc cagcgagacg ggcccagtgg 60
ctgtcgaccc aacgctgcgc aggcgcatcg agccgcacga gttcgaggtc ttcttcgacc 120
ccagggagct gcgcaaggag acgtgcctcc tgtacgagat caactggggc ggcaggcact 180
ccatctggag gcacaccagc cagaacacga acaagcacgt ggaggtcaac ttcatcgaga 240
agttcaccac ggagaggtac ttctgcccga acacccgctg ctccatcacg tggttcctgt 300
cctggagccc ctgcggcgag tgctccaggg cgatcaccga gttcctcagc cgctacccgc 360
acgtgacgct gttcatctac atcgctaggc tctaccacca cgctgacccc aggaacaggc 420
agggcctccg cgacctgatc tccagcggcg tgaccatcca gatcatgacg gagcaggagt 480
ccggctactg ctggaggaac ttcgtcaact actccccaag caacgaggct cactggccga 540
ggtacccaca cctctgggtg cgcctctacg tgctcgagct gtactgcatc atcctcggcc 600
tgccgccctg cctcaacatc ctgaggcgca agcagcccca gctgaccttc ttcacgatcg 660
ccctccagag ctgccactac cagaggctcc caccacacat cctgtgggcg accggcctca 720
agtccggcag cgagacgcca ggcacgtccg agagcgctac gccagagctg aaggacaaga 780
agtactcgat cggcctcgcc attgggacta actctgttgg ctgggccgtg atcaccgacg 840
agtacaaggt gccctcaaag aagttcaagg tcctgggcaa caccgatcgg cattccatca 900
agaagaatct cattggcgct ctcctgttcg acagcggcga gacggctgag gctacgcggc 960
tcaagcgcac cgcccgcagg cggtacacgc gcaggaagaa tcgcatctgc tacctgcagg 1020
agattttctc caacgagatg gcgaaggttg acgattcttt cttccacagg ctggaggagt 1080
cattcctcgt ggaggaggat aagaagcacg agcggcatcc aatcttcggc aacattgtcg 1140
acgaggttgc ctaccacgag aagtacccta cgatctacca tctgcggaag aagctcgtgg 1200
actccacaga taaggcggac ctccgcctga tctacctcgc tctggcccac atgattaagt 1260
tcaggggcca tttcctgatc gagggggatc tcaacccgga caatagcgat gttgacaagc 1320
tgttcatcca gctcgtgcag acgtacaacc agctcttcga ggagaacccc attaatgcgt 1380
caggcgtcga cgcgaaggct atcctgtccg ctaggctctc gaagtctcgg cgcctcgaga 1440
acctgatcgc ccagctgccg ggcgagaaga agaacggcct gttcgggaat ctcattgcgc 1500
tcagcctggg gctcacgccc aacttcaagt cgaatttcga tctcgctgag gacgccaagc 1560
tgcagctctc caaggacaca tacgacgatg acctggataa cctcctggcc cagatcggcg 1620
atcagtacgc ggacctgttc ctcgctgcca agaatctgtc ggacgccatc ctcctgtctg 1680
atattctcag ggtgaacacc gagattacga aggctccgct ctcagcctcc atgatcaagc 1740
gctacgacga gcaccatcag gatctgaccc tcctgaaggc gctggtcagg cagcagctcc 1800
ccgagaagta caaggagatc ttcttcgatc agtcgaagaa cggctacgct gggtacattg 1860
acggcggggc ctctcaggag gagttctaca agttcatcaa gccgattctg gagaagatgg 1920
acggcacgga ggagctgctg gtgaagctca atcgcgagga cctcctgagg aagcagcgga 1980
cattcgataa cggcagcatc ccacaccaga ttcatctcgg ggagctgcac gctatcctga 2040
ggaggcagga ggacttctac cctttcctca aggataaccg cgagaagatc gagaagattc 2100
tgactttcag gatcccgtac tacgtcggcc cactcgctag gggcaactcc cgcttcgctt 2160
ggatgacccg caagtcagag gagacgatca cgccgtggaa cttcgaggag gtggtcgaca 2220
agggcgctag cgctcagtcg ttcatcgaga ggatgacgaa tttcgacaag aacctgccaa 2280
atgagaaggt gctccctaag cactcgctcc tgtacgagta cttcacagtc tacaacgagc 2340
tgactaaggt gaagtatgtg accgagggca tgaggaagcc ggctttcctg tctggggagc 2400
agaagaaggc catcgtggac ctcctgttca agaccaaccg gaaggtcacg gttaagcagc 2460
tcaaggagga ctacttcaag aagattgagt gcttcgattc ggtcgagatc tctggcgttg 2520
aggaccgctt caacgcctcc ctggggacct accacgatct cctgaagatc attaaggata 2580
aggacttcct ggacaacgag gagaatgagg atatcctcga ggacattgtg ctgacactca 2640
ctctgttcga ggaccgggag atgatcgagg agcgcctgaa gacttacgcc catctcttcg 2700
atgacaaggt catgaagcag ctcaagagga ggaggtacac cggctggggg aggctgagca 2760
ggaagctcat caacggcatt cgggacaagc agtccgggaa gacgatcctc gacttcctga 2820
agagcgatgg cttcgcgaac cgcaatttca tgcagctgat tcacgatgac agcctcacat 2880
tcaaggagga tatccagaag gctcaggtga gcggccaggg ggactcgctg cacgagcata 2940
tcgcgaacct cgctggctcg ccagctatca agaaggggat tctgcagacc gtgaaggttg 3000
tggacgagct ggtgaaggtc atgggcaggc acaagcctga gaacatcgtc attgagatgg 3060
cccgggagaa tcagaccacg cagaagggcc agaagaactc acgcgagagg atgaagagga 3120
tcgaggaggg cattaaggag ctggggtccc agatcctcaa ggagcacccg gtggagaaca 3180
cgcagctgca gaatgagaag ctctacctgt actacctcca gaatggccgc gatatgtatg 3240
tggaccagga gctggatatt aacaggctca gcgattacga cgtcgatcat atcgttccac 3300
agtcattcct gaaggatgac tccattgaca acaaggtcct caccaggtcg gacaagaacc 3360
ggggcaagtc tgataatgtt ccttcagagg aggtcgttaa gaagatgaag aactactggc 3420
gccagctcct gaatgccaag ctgatcacgc agcggaagtt cgataacctc acaaaggctg 3480
agaggggcgg gctctctgag ctggacaagg cgggcttcat caagaggcag ctggtcgaga 3540
cacggcagat cactaagcac gttgcgcaga ttctcgactc acggatgaac actaagtacg 3600
atgagaatga caagctgatc cgcgaggtga aggtcatcac cctgaagtca aagctcgtct 3660
ccgacttcag gaaggatttc cagttctaca aggttcggga gatcaacaat taccaccatg 3720
cccatgacgc gtacctgaac gcggtggtcg gcacagctct gatcaagaag tacccaaagc 3780
tcgagagcga gttcgtgtac ggggactaca aggtttacga tgtgaggaag atgatcgcca 3840
agtcggagca ggagattggc aaggctaccg ccaagtactt cttctactct aacattatga 3900
atttcttcaa gacagagatc actctggcca atggcgagat ccggaagcgc cccctcatcg 3960
agacgaacgg cgagacgggg gagatcgtgt gggacaaggg cagggatttc gcgaccgtca 4020
ggaaggttct ctccatgcca caagtgaata tcgtcaagaa gacagaggtc cagactggcg 4080
ggttctctaa ggagtcaatt ctgcctaagc ggaacagcga caagctcatc gcccgcaaga 4140
aggactggga tccgaagaag tacggcgggt tcgacagccc cactgtggcc tactcggtcc 4200
tggttgtggc gaaggttgag aagggcaagt ccaagaagct caagagcgtg aaggagctgc 4260
tggggatcac gattatggag cgctccagct tcgagaagaa cccgatcgat ttcctggagg 4320
cgaagggcta caaggaggtg aagaaggacc tgatcattaa gctccccaag tactcactct 4380
tcgagctgga gaacggcagg aagcggatgc tggcttccgc tggcgagctg cagaagggga 4440
acgagctggc tctgccgtcc aagtatgtga acttcctcta cctggcctcc cactacgaga 4500
agctcaaggg cagccccgag gacaacgagc agaagcagct gttcgtcgag cagcacaagc 4560
attacctcga cgagatcatt gagcagattt ccgagttctc caagcgcgtg atcctggccg 4620
acgcgaatct ggataaggtc ctctccgcgt acaacaagca ccgcgacaag ccaatcaggg 4680
agcaggctga gaatatcatt catctcttca ccctgacgaa cctcggcgcc cctgctgctt 4740
tcaagtactt cgacacaact atcgatcgca agaggtacac aagcactaag gaggtcctgg 4800
acgcgaccct catccaccag tcgattaccg gcctctacga gacgcgcatc gacctgtctc 4860
agctcggggg cgacaagcgg ccagcggcga cgaagaaggc ggggcaggcg aagaagaaga 4920
agacccgcga ctccggcggc agcacgaacc tctccgacat catcgagaag gagacgggca 4980
agcagctcgt gatccaggag agcatcctca tgctgccgga ggaggtggag gaggtcatcg 5040
gcaacaagcc cgagtccgac atcctcgtgc acaccgccta cgacgagtcc acggacgaga 5100
acgtcatgct cctgacgagc gacgctccag agtacaagcc atgggctctc gtgatccagg 5160
acagcaacgg cgagaacaag atcaagatgc tgtccggcgg ctccccgaag aagaagcgca 5220
aggtctgagc tcatcggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 5280
aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 5340
aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5400
ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5460
tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 5520
agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5580
gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5640
tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5700
acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 5760
tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 5820
caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 5880
aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 5940
aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 6000
ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 6060
agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 6120
atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 6180
cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 6240
aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 6300
cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6360
aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6420
ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 6480
gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6540
ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6600
tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6660
tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg 6720
ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 6780
tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 6840
agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 6900
acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 6960
ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 7020
gttccgcgca catttccccg aaaagtgcca cctgacgtc 7059
<210> 133
<211> 18803
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE vector
<400> 133
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtgagaccaa 720
cccagtggac ataagcctgt tcggttcgta agctgtaatg caagtagcgt atgcgctcac 780
gcaactggtc cagaaccttg accgaacgca gcggtggtaa cggcgcagtg gcggttttca 840
tggcttgtta tgactgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag 900
cgcgttacgc cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg 960
gcagtcgccc taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga 1020
ctcaactatc agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg 1080
tacatttgta cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc 1140
tggttacggt gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt 1200
tggaaacttc ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg 1260
ttgtgcacga cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag 1320
aatggcagcg caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc 1380
tggctatctt gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg 1440
aggaactctt tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa 1500
cgctatggaa ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt 1560
cccgcatttg gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact 1620
gggcaatgga gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt 1680
atcttggaca agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc 1740
actacgtgaa aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt 1800
tcaagccgac gccgcttcgc ggcgcggctt aactcaagcg ttagatgcac taagcacata 1860
attgctcaca gccaaactat caggtcaagt ctgcttttat tatttttaag cgtgcataat 1920
aagccggtct cggttttaga gctagaaata gcaagttaaa ataaggctag tccgttatca 1980
acttgaaaaa gtggcaccga gtcggtgctt ttttttttcg ttttgcattg agttttctcc 2040
gtcgcatgtt tgcagtttta ttttccgttt tgcattgaaa tttctccgtc tcatgtttgc 2100
agcgtgttca aaaagtacgc agctgtattt cacttattta cggcgccaca ttttcatgcc 2160
gtttgtgcca actatcccga gctagtgaat acagcttggc ttcacacaac actggtgacc 2220
cgctgacctg ctcgtacctc gtaccgtcgt acggcacagc atttggaatt aaagggtgtg 2280
atcgatactg cttgctgcta agcttgcatg cctgcagtgc agcgtgaccc ggtcgtgccc 2340
ctctctagag ataatgagca ttgcatgtct aagttataaa aaattaccac atattttttt 2400
tgtcacactt gtttgaagtg cagtttatct atctttatac atatatttaa actttactct 2460
acgaataata taatctatag tactacaata atatcagtgt tttagagaat catataaatg 2520
aacagttaga catggtctaa aggacaattg agtattttga caacaggact ctacagtttt 2580
atctttttag tgtgcatgtg ttctcctttt tttttgcaaa tagcttcacc tatataatac 2640
ttcatccatt ttattagtac atccatttag ggtttagggt taatggtttt tatagactaa 2700
tttttttagt acatctattt tattctattt tagcctctaa attaagaaaa ctaaaactct 2760
attttagttt ttttatttaa taatttagat ataaaataga ataaaataaa gtgactaaaa 2820
attaaacaaa taccctttaa gaaattaaaa aaactaagga aacatttttc ttgtttcgag 2880
tagataatgc cagcctgtta aacgccgtcg acgagtctaa cggacaccaa ccagcgaacc 2940
agcagcgtcg cgtcgggcca agcgaagcag acggcacggc atctctgtcg ctgcctctgg 3000
acccctctcg agagttccgc tccaccgttg gacttgctcc gctgtcggca tccagaaatt 3060
gcgtggcgga gcggcagacg tgagccggca cggcaggcgg cctcctcctc ctctcacggc 3120
acggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg 3180
taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac 3240
acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc 3300
gctcgtcctc cccccccccc cctctctacc ttctctagat cggcgttccg gtccatggtt 3360
agggcccggt agttctactt ctgttcatgt ttgtgttaga tccgtgtttg tgttagatcc 3420
gtgctgctag cgttcgtaca cggatgcgac ctgtacgtca gacacgttct gattgctaac 3480
ttgccagtgt ttctctttgg ggaatcctgg gatggctcta gccgttccgc agacgggatc 3540
gatttcatga ttttttttgt ttcgttgcat agggtttggt ttgccctttt cctttatttc 3600
aatatatgcc gtgcacttgt ttgtcgggtc atcttttcat gctttttttt gtcttggttg 3660
tgatgatgtg gtctggttgg gcggtcgttc tagatcggag tagaattctg tttcaaacta 3720
cctggtggat ttattaattt tggatctgta tgtgtgtgcc atacatattc atagttacga 3780
attgaagatg atggatggaa atatcgatct aggataggta tacatgttga tgcgggtttt 3840
actgatgcat atacagagat gctttttgtt cgcttggttg tgatgatgtg gtgtggttgg 3900
gcggtcgttc attcgttcta gatcggagta gaatactgtt tcaaactacc tggtgtattt 3960
attaattttg gaactgtatg tgtgtgtcat acatcttcat agttacgagt ttaagatgga 4020
tggaaatatc gatctaggat aggtatacat gttgatgtgg gttttactga tgcatataca 4080
tgatggcata tgcagcatct attcatatgc tctaaccttg agtacctatc tattataata 4140
aacaagtatg ttttataatt attttgatct tgatatactt ggatgatggc atatgcagca 4200
gctatatgtg gattttttta gccctgcctt catacgctat ttatttgctt ggtactgttt 4260
cttttgtcga tgctcaccct gttgtttggt gttacttctg cagccctagg atgccgaaga 4320
agaagcgcaa ggtgtccagc gagacgggcc cagtggctgt cgacccaacg ctgcgcaggc 4380
gcatcgagcc gcacgagttc gaggtcttct tcgaccccag ggagctgcgc aaggagacgt 4440
gcctcctgta cgagatcaac tggggcggca ggcactccat ctggaggcac accagccaga 4500
acacgaacaa gcacgtggag gtcaacttca tcgagaagtt caccacggag aggtacttct 4560
gcccgaacac ccgctgctcc atcacgtggt tcctgtcctg gagcccctgc ggcgagtgct 4620
ccagggcgat caccgagttc ctcagccgct acccgcacgt gacgctgttc atctacatcg 4680
ctaggctcta ccaccacgct gaccccagga acaggcaggg cctccgcgac ctgatctcca 4740
gcggcgtgac catccagatc atgacggagc aggagtccgg ctactgctgg aggaacttcg 4800
tcaactactc cccaagcaac gaggctcact ggccgaggta cccacacctc tgggtgcgcc 4860
tctacgtgct cgagctgtac tgcatcatcc tcggcctgcc gccctgcctc aacatcctga 4920
ggcgcaagca gccccagctg accttcttca cgatcgccct ccagagctgc cactaccaga 4980
ggctcccacc acacatcctg tgggcgaccg gcctcaagtc cggcagcgag acgccaggca 5040
cgtccgagag cgctacgcca gagctgaagg acaagaagta ctcgatcggc ctcgccattg 5100
ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc tcaaagaagt 5160
tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc 5220
tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt 5280
acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac gagatggcga 5340
aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag gaggataaga 5400
agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt 5460
accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag gcggacctcc 5520
gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc ctgatcgagg 5580
gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc gtgcagacgt 5640
acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc 5700
tgtccgctag gctctcgaag tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg 5760
agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc acgcccaact 5820
tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag gacacatacg 5880
acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac ctgttcctcg 5940
ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg aacaccgaga 6000
ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac catcaggatc 6060
tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag gagatcttct 6120
tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct caggaggagt 6180
tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag ctgctggtga 6240
agctcaatcg cgaggacctc ctgaggaagc agcggacatt cgataacggc agcatcccac 6300
accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac ttctaccctt 6360
tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc ccgtactacg 6420
tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag tcagaggaga 6480
cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca 6540
tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc cctaagcact 6600
cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag tatgtgaccg 6660
agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc gtggacctcc 6720
tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac ttcaagaaga 6780
ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac gcctccctgg 6840
ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac aacgaggaga 6900
atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac cgggagatga 6960
tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg aagcagctca 7020
agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac ggcattcggg 7080
acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc gcgaaccgca 7140
atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc cagaaggctc 7200
aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct ggctcgccag 7260
ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg aaggtcatgg 7320
gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag accacgcaga 7380
agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt aaggagctgg 7440
ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat gagaagctct 7500
acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg gatattaaca 7560
ggctcagcga ttacgacgtc gatcatatcg ttccacagtc attcctgaag gatgactcca 7620
ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat aatgttcctt 7680
cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat gccaagctga 7740
tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc tctgagctgg 7800
acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact aagcacgttg 7860
cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag ctgatccgcg 7920
aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag gatttccagt 7980
tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac ctgaacgcgg 8040
tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc gtgtacgggg 8100
actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag attggcaagg 8160
ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca gagatcactc 8220
tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag acgggggaga 8280
tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc atgccacaag 8340
tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag tcaattctgc 8400
ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg aagaagtacg 8460
gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag gttgagaagg 8520
gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt atggagcgct 8580
ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag gaggtgaaga 8640
aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac ggcaggaagc 8700
ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg ccgtccaagt 8760
atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc cccgaggaca 8820
acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag atcattgagc 8880
agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat aaggtcctct 8940
ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat atcattcatc 9000
tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac acaactatcg 9060
atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc caccagtcga 9120
ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac aagcggccag 9180
cggcgacgaa gaaggcgggg caggcgaaga agaagaagac ccgcgactcc ggcggcagca 9240
cgaacctctc cgacatcatc gagaaggaga cgggcaagca gctcgtgatc caggagagca 9300
tcctcatgct gccggaggag gtggaggagg tcatcggcaa caagcccgag tccgacatcc 9360
tcgtgcacac cgcctacgac gagtccacgg acgagaacgt catgctcctg acgagcgacg 9420
ctccagagta caagccatgg gctctcgtga tccaggacag caacggcgag aacaagatca 9480
agatgctgtc cggcggctcc ccgaagaaga agcgcaaggt ctgagctcag agctttcgtt 9540
cgtatcatcg gtttcgacaa cgttcgtcaa gttcaatgca tcagtttcat tgcgcacaca 9600
ccagaatcct actgagtttg agtattatgg cattgggaaa actgtttttc ttgtaccatt 9660
tgttgtgctt gtaatttact gtgtttttta ttcggttttc gctatcgaac tgtgaaatgg 9720
aaatggatgg agaagagtta atgaatgata tggtcctttt gttcattctc aaattaatat 9780
tatttgtttt ttctcttatt tgttgtgtgt tgaatttgaa attataagag atatgcaaac 9840
attttgtttt gagtaaaaat gtgtcaaatc gtggcctcta atgaccgaag ttaatatgag 9900
gagtaaaaca cttgtagttg taccattatg cttattcact aggcaacaaa tatattttca 9960
gacctagaaa agctgcaaat gttactgaat acaagtatgt cctcttgtgt tttagacatt 10020
tatgaacttt cctttatgta attttccaga atccttgtca gattctaatc attgctttat 10080
aattatagtt atactcatgg atttgtagtt gagtatgaaa atatttttta atgcatttta 10140
tgacttgcca attgattgac aacgaattcg taatcatgtc atagctgttt cctgtgtgaa 10200
attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct 10260
ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc 10320
agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg 10380
gtttgcgtat tggctagagc agcttgccaa catggtggag cacgacactc tcgtctactc 10440
caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag 10500
ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag 10560
gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat 10620
cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat 10680
cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgataacat 10740
ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca 10800
aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg 10860
cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg 10920
ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa 10980
agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc 11040
aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta 11100
tccttcgcaa gaccttcctc tatataagga agttcatttc atttggagag gacacgctga 11160
aatcaccagt ctctctctac aaatctatct ctctcgagct ttcgcagatc ccggggggca 11220
atgagatatg aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa 11280
gttcgacagc gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag 11340
cttcgatgta ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta 11400
caaagatcgt tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct 11460
tgacattggg gagtttagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt 11520
cacgttgcaa gacctgcctg aaaccgaact gcccgctgtt ctacaaccgg tcgcggaggc 11580
tatggatgcg atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc 11640
gcaaggaatc ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca 11700
tgtgtatcac tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct 11760
cgatgagctg atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga 11820
tttcggctcc aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag 11880
cgaggcgatg ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg 11940
gttggcttgt atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg 12000
atcgccacga ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt 12060
ggttgacggc aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg 12120
atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac 12180
cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag 12240
ggcaaagaaa tagagtagat gccgaccgga tctgtcgatc gacaagctcg agtttctcca 12300
taataatgtg tgagtagttc ccagataagg gaattagggt tcctataggg tttcgctcat 12360
gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat 12420
aaaatttcta attcctaaaa ccaaaatcca gtactaaaat ccagatcccc cgaattaatt 12480
cggcgttaat tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca 12540
atttgtttac accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc 12600
tcggcacaaa atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga 12660
gagccgttgt aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa 12720
ctaagctgcc gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg 12780
atgacagagc gttgctgcct gtgatcaccg cggtttcaaa atcggctccg tcgatactat 12840
gttatacgcc aactttgaaa acaactttga aaaagctgtt ttctggtatt taaggtttta 12900
gaatgcaagg aacagtgaat tggagttcgt cttgttataa ttagcttctt ggggtatctt 12960
taaatactgt agaaaagagg aaggaaataa taaatggcta aaatgagaat atcaccggaa 13020
ttgaaaaaac tgatcgaaaa ataccgctgc gtaaaagata cggaaggaat gtctcctgct 13080
aaggtatata agctggtggg agaaaatgaa aacctatatt taaaaatgac ggacagccgg 13140
tataaaggga ccacctatga tgtggaacgg gaaaaggaca tgatgctatg gctggaagga 13200
aagctgcctg ttccaaaggt cctgcacttt gaacggcatg atggctggag caatctgctc 13260
atgagtgagg ccgatggcgt cctttgctcg gaagagtatg aagatgaaca aagccctgaa 13320
aagattatcg agctgtatgc ggagtgcatc aggctctttc actccatcga catatcggat 13380
tgtccctata cgaatagctt agacagccgc ttagccgaat tggattactt actgaataac 13440
gatctggccg atgtggattg cgaaaactgg gaagaagaca ctccatttaa agatccgcgc 13500
gagctgtatg attttttaaa gacggaaaag cccgaagagg aacttgtctt ttcccacggc 13560
gacctgggag acagcaacat ctttgtgaaa gatggcaaag taagtggctt tattgatctt 13620
gggagaagcg gcagggcgga caagtggtat gacattgcct tctgcgtccg gtcgatcagg 13680
gaggatatcg gggaagaaca gtatgtcgag ctattttttg acttactggg gatcaagcct 13740
gattgggaga aaataaaata ttatatttta ctggatgaat tgttttagta cctagaatgc 13800
atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag 13860
atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa 13920
aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg 13980
aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag 14040
ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg 14100
ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga 14160
tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc 14220
ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc 14280
acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga 14340
gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt 14400
cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg 14460
aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac 14520
atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga 14580
gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg 14640
gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata 14700
tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc 14760
tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc 14820
cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga 14880
gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga 14940
tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct 15000
ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg 15060
gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc 15120
tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag 15180
ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg 15240
ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt 15300
gggttcccaa tgtacgtgct atccacagga aacagacctt ttcgaccttt ttcccctgct 15360
agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg 15420
atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa 15480
tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg 15540
aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc 15600
ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa 15660
aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac 15720
atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc 15780
ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc 15840
ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg 15900
tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt 15960
ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg 16020
gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc 16080
ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct 16140
cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg 16200
gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc 16260
ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca 16320
gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg 16380
gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc 16440
gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat 16500
tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca 16560
tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag 16620
ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg 16680
tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg 16740
gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg 16800
ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt 16860
gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag 16920
cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg 16980
tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg 17040
gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg 17100
cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa 17160
ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca 17220
gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt 17280
cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt 17340
cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc 17400
cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta 17460
gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct 17520
tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc 17580
atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc 17640
ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat 17700
ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat 17760
catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg 17820
tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc 17880
aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg 17940
cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc 18000
acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg 18060
gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg 18120
cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg 18180
cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg 18240
gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct 18300
agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc 18360
gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg 18420
tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc 18480
agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta 18540
ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca 18600
gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa 18660
cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga 18720
ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc ttttcacgcc 18780
cttttaaata tccgttattc taa 18803
<210> 134
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R1 construct
<400> 134
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccagtgctt 720
attctagggc atatgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 135
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R2 construct
<400> 135
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccggtgcat 720
acagcgtctt gaccgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 136
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R4 construct
<400> 136
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtctgcactg 720
aacaagcttc ttgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 137
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R5 construct
<400> 137
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccacatgca 720
gttgggtggt cccagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 138
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R6 construct
<400> 138
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccatcttac 720
tgtttcagat gaccgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 139
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R7 construct
<400> 139
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccctgctga 720
ccctggtcag cttggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 140
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R8 construct
<400> 140
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gttcctcgtg 720
ctggacaagt gtgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 141
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R10 construct
<400> 141
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcaagactgc 720
gcaggcattg ctgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 142
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R11 construct
<400> 142
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcctcgctaa 720
ctggagaggc ttctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 143
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R12 construct
<400> 143
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcgactattg 720
ttgagaacct tagggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 144
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R13 construct
<400> 144
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccatggctg 720
cagagctacg aggagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 145
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R14 construct
<400> 145
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccgcattga 720
gtgctatgct gagagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 146
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R15 construct
<400> 146
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtatgctgag 720
aggactgcaa aagggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 147
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R16 construct
<400> 147
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccaggattg 720
catgagtcgg cttggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 148
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R17 construct
<400> 148
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gggagcttat 720
cttgctcgac ttgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 149
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R18 construct
<400> 149
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccgcaaggg 720
ttaattgaga tcaagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 150
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R19 construct
<400> 150
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtgcttattc 720
tagggcatat aagggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 151
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R20 construct
<400> 151
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtttacactt 720
acatttgtga ctgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 152
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R21 construct
<400> 152
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gagctcccac 720
atgcagttgg gtgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 153
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R22 construct
<400> 153
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gactgtttca 720
gatgaccttg aagggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 154
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R23 construct
<400> 154
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc ggcgtttcta 720
atatattgag gtgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 155
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R24 construct
<400> 155
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gtatgttcct 720
gcctacattg gtgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 156
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R25 construct
<400> 156
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gacttccagt 720
aacaacaccg ttgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 157
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R26 construct
<400> 157
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gaacaacacc 720
gttggaccca ccgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 158
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R27 construct
<400> 158
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc ggaactcgtg 720
tgatcctcga gcgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 159
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R28 construct
<400> 159
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc ggttactggc 720
agagcaaagc ttgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 160
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R29 construct
<400> 160
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcaaactatc 720
cctgctgacc ctgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 161
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R30 construct
<400> 161
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gatggctgca 720
gagctacgag gagggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 162
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R31 construct
<400> 162
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc ggactgcaaa 720
aggcaatgtt ctgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 163
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R32 construct
<400> 163
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcccagaccg 720
cattgagtgc tatggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 164
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R33 construct
<400> 164
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcctttgtct 720
acattcccat ggctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 165
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R34 construct
<400> 165
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccagtgggt 720
gtgatagctg tggagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 166
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R35 construct
<400> 166
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccaagggaa 720
atggttaggt ggtagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 167
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R36 construct
<400> 167
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcctcgagcg 720
gctatccgtg gtgtgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 168
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R37 construct
<400> 168
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcctgagaac 720
tcgtgtgatc ctcggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 169
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R38 construct
<400> 169
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcctgttgca 720
tacattcctg agaagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 170
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R39 construct
<400> 170
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gccgttggac 720
ccaccggaca gaccgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 171
<211> 17605
<212> DNA
<213> Artificial Sequence
<220>
<223> pZRH-PBE-R40 construct
<400> 171
taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta 60
aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc 120
aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg 180
gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca 240
cgacgttgta aaacgacggc cagtgccaag cttagtaatt catccaggtc accaagttct 300
aggattttca gaactgcaac ttattttatc aaggaatctt taaacatacg aacagatcac 360
ttaaagttct tctgaagcaa cttaaagtta tcaggcatgc atggatcttg gaggaatcag 420
atgtgcagtc agggaccata gcacaagaca ggcgtcttct actggtgcta ccagcaaatg 480
ctggaagccg ggaacactgg gtacgttgga aaccacgtga tgtgaagaag taagataaac 540
tgtaggagaa aagcatttcg tagtgggcca tgaagccttt caggacatgt attgcagtat 600
gggccggccc attacgcaat tggacgacaa caaagactag tattagtacc acctcggcta 660
tccacataga tcaaagctga tttaaaagag ttgtgcagat gatccgtggc gcctattatt 720
cttacaggct attcgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat 780
caacttgaaa aagtggcacc gagtcggtgc tttttttttt cgttttgcat tgagttttct 840
ccgtcgcatg tttgcagttt tattttccgt tttgcattga aatttctccg tctcatgttt 900
gcagcgtgtt caaaaagtac gcagctgtat ttcacttatt tacggcgcca cattttcatg 960
ccgtttgtgc caactatccc gagctagtga atacagcttg gcttcacaca acactggtga 1020
cccgctgacc tgctcgtacc tcgtaccgtc gtacggcaca gcatttggaa ttaaagggtg 1080
tgatcgatac tgcttgctgc taagcttgca tgcctgcagt gcagcgtgac ccggtcgtgc 1140
ccctctctag agataatgag cattgcatgt ctaagttata aaaaattacc acatattttt 1200
tttgtcacac ttgtttgaag tgcagtttat ctatctttat acatatattt aaactttact 1260
ctacgaataa tataatctat agtactacaa taatatcagt gttttagaga atcatataaa 1320
tgaacagtta gacatggtct aaaggacaat tgagtatttt gacaacagga ctctacagtt 1380
ttatcttttt agtgtgcatg tgttctcctt tttttttgca aatagcttca cctatataat 1440
acttcatcca ttttattagt acatccattt agggtttagg gttaatggtt tttatagact 1500
aattttttta gtacatctat tttattctat tttagcctct aaattaagaa aactaaaact 1560
ctattttagt ttttttattt aataatttag atataaaata gaataaaata aagtgactaa 1620
aaattaaaca aatacccttt aagaaattaa aaaaactaag gaaacatttt tcttgtttcg 1680
agtagataat gccagcctgt taaacgccgt cgacgagtct aacggacacc aaccagcgaa 1740
ccagcagcgt cgcgtcgggc caagcgaagc agacggcacg gcatctctgt cgctgcctct 1800
ggacccctct cgagagttcc gctccaccgt tggacttgct ccgctgtcgg catccagaaa 1860
ttgcgtggcg gagcggcaga cgtgagccgg cacggcaggc ggcctcctcc tcctctcacg 1920
gcacggcagc tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc 1980
cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc 2040
acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg 2100
ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg 2160
ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat 2220
ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta 2280
acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga 2340
tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt 2400
tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt 2460
tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac 2520
tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac 2580
gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt 2640
ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt 2700
gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat 2760
ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg 2820
gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata 2880
catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa 2940
taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag 3000
cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt 3060
ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta ggatgccgaa 3120
gaagaagcgc aaggtgtcca gcgagacggg cccagtggct gtcgacccaa cgctgcgcag 3180
gcgcatcgag ccgcacgagt tcgaggtctt cttcgacccc agggagctgc gcaaggagac 3240
gtgcctcctg tacgagatca actggggcgg caggcactcc atctggaggc acaccagcca 3300
gaacacgaac aagcacgtgg aggtcaactt catcgagaag ttcaccacgg agaggtactt 3360
ctgcccgaac acccgctgct ccatcacgtg gttcctgtcc tggagcccct gcggcgagtg 3420
ctccagggcg atcaccgagt tcctcagccg ctacccgcac gtgacgctgt tcatctacat 3480
cgctaggctc taccaccacg ctgaccccag gaacaggcag ggcctccgcg acctgatctc 3540
cagcggcgtg accatccaga tcatgacgga gcaggagtcc ggctactgct ggaggaactt 3600
cgtcaactac tccccaagca acgaggctca ctggccgagg tacccacacc tctgggtgcg 3660
cctctacgtg ctcgagctgt actgcatcat cctcggcctg ccgccctgcc tcaacatcct 3720
gaggcgcaag cagccccagc tgaccttctt cacgatcgcc ctccagagct gccactacca 3780
gaggctccca ccacacatcc tgtgggcgac cggcctcaag tccggcagcg agacgccagg 3840
cacgtccgag agcgctacgc cagagctgaa ggacaagaag tactcgatcg gcctcgccat 3900
tgggactaac tctgttggct gggccgtgat caccgacgag tacaaggtgc cctcaaagaa 3960
gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag aagaatctca ttggcgctct 4020
cctgttcgac agcggcgaga cggctgaggc tacgcggctc aagcgcaccg cccgcaggcg 4080
gtacacgcgc aggaagaatc gcatctgcta cctgcaggag attttctcca acgagatggc 4140
gaaggttgac gattctttct tccacaggct ggaggagtca ttcctcgtgg aggaggataa 4200
gaagcacgag cggcatccaa tcttcggcaa cattgtcgac gaggttgcct accacgagaa 4260
gtaccctacg atctaccatc tgcggaagaa gctcgtggac tccacagata aggcggacct 4320
ccgcctgatc tacctcgctc tggcccacat gattaagttc aggggccatt tcctgatcga 4380
gggggatctc aacccggaca atagcgatgt tgacaagctg ttcatccagc tcgtgcagac 4440
gtacaaccag ctcttcgagg agaaccccat taatgcgtca ggcgtcgacg cgaaggctat 4500
cctgtccgct aggctctcga agtctcggcg cctcgagaac ctgatcgccc agctgccggg 4560
cgagaagaag aacggcctgt tcgggaatct cattgcgctc agcctggggc tcacgcccaa 4620
cttcaagtcg aatttcgatc tcgctgagga cgccaagctg cagctctcca aggacacata 4680
cgacgatgac ctggataacc tcctggccca gatcggcgat cagtacgcgg acctgttcct 4740
cgctgccaag aatctgtcgg acgccatcct cctgtctgat attctcaggg tgaacaccga 4800
gattacgaag gctccgctct cagcctccat gatcaagcgc tacgacgagc accatcagga 4860
tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc gagaagtaca aggagatctt 4920
cttcgatcag tcgaagaacg gctacgctgg gtacattgac ggcggggcct ctcaggagga 4980
gttctacaag ttcatcaagc cgattctgga gaagatggac ggcacggagg agctgctggt 5040
gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca ttcgataacg gcagcatccc 5100
acaccagatt catctcgggg agctgcacgc tatcctgagg aggcaggagg acttctaccc 5160
tttcctcaag gataaccgcg agaagatcga gaagattctg actttcagga tcccgtacta 5220
cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg atgacccgca agtcagagga 5280
gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag ggcgctagcg ctcagtcgtt 5340
catcgagagg atgacgaatt tcgacaagaa cctgccaaat gagaaggtgc tccctaagca 5400
ctcgctcctg tacgagtact tcacagtcta caacgagctg actaaggtga agtatgtgac 5460
cgagggcatg aggaagccgg ctttcctgtc tggggagcag aagaaggcca tcgtggacct 5520
cctgttcaag accaaccgga aggtcacggt taagcagctc aaggaggact acttcaagaa 5580
gattgagtgc ttcgattcgg tcgagatctc tggcgttgag gaccgcttca acgcctccct 5640
ggggacctac cacgatctcc tgaagatcat taaggataag gacttcctgg acaacgagga 5700
gaatgaggat atcctcgagg acattgtgct gacactcact ctgttcgagg accgggagat 5760
gatcgaggag cgcctgaaga cttacgccca tctcttcgat gacaaggtca tgaagcagct 5820
caagaggagg aggtacaccg gctgggggag gctgagcagg aagctcatca acggcattcg 5880
ggacaagcag tccgggaaga cgatcctcga cttcctgaag agcgatggct tcgcgaaccg 5940
caatttcatg cagctgattc acgatgacag cctcacattc aaggaggata tccagaaggc 6000
tcaggtgagc ggccaggggg actcgctgca cgagcatatc gcgaacctcg ctggctcgcc 6060
agctatcaag aaggggattc tgcagaccgt gaaggttgtg gacgagctgg tgaaggtcat 6120
gggcaggcac aagcctgaga acatcgtcat tgagatggcc cgggagaatc agaccacgca 6180
gaagggccag aagaactcac gcgagaggat gaagaggatc gaggagggca ttaaggagct 6240
ggggtcccag atcctcaagg agcacccggt ggagaacacg cagctgcaga atgagaagct 6300
ctacctgtac tacctccaga atggccgcga tatgtatgtg gaccaggagc tggatattaa 6360
caggctcagc gattacgacg tcgatcatat cgttccacag tcattcctga aggatgactc 6420
cattgacaac aaggtcctca ccaggtcgga caagaaccgg ggcaagtctg ataatgttcc 6480
ttcagaggag gtcgttaaga agatgaagaa ctactggcgc cagctcctga atgccaagct 6540
gatcacgcag cggaagttcg ataacctcac aaaggctgag aggggcgggc tctctgagct 6600
ggacaaggcg ggcttcatca agaggcagct ggtcgagaca cggcagatca ctaagcacgt 6660
tgcgcagatt ctcgactcac ggatgaacac taagtacgat gagaatgaca agctgatccg 6720
cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc gacttcagga aggatttcca 6780
gttctacaag gttcgggaga tcaacaatta ccaccatgcc catgacgcgt acctgaacgc 6840
ggtggtcggc acagctctga tcaagaagta cccaaagctc gagagcgagt tcgtgtacgg 6900
ggactacaag gtttacgatg tgaggaagat gatcgccaag tcggagcagg agattggcaa 6960
ggctaccgcc aagtacttct tctactctaa cattatgaat ttcttcaaga cagagatcac 7020
tctggccaat ggcgagatcc ggaagcgccc cctcatcgag acgaacggcg agacggggga 7080
gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg aaggttctct ccatgccaca 7140
agtgaatatc gtcaagaaga cagaggtcca gactggcggg ttctctaagg agtcaattct 7200
gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag gactgggatc cgaagaagta 7260
cggcgggttc gacagcccca ctgtggccta ctcggtcctg gttgtggcga aggttgagaa 7320
gggcaagtcc aagaagctca agagcgtgaa ggagctgctg gggatcacga ttatggagcg 7380
ctccagcttc gagaagaacc cgatcgattt cctggaggcg aagggctaca aggaggtgaa 7440
gaaggacctg atcattaagc tccccaagta ctcactcttc gagctggaga acggcaggaa 7500
gcggatgctg gcttccgctg gcgagctgca gaaggggaac gagctggctc tgccgtccaa 7560
gtatgtgaac ttcctctacc tggcctccca ctacgagaag ctcaagggca gccccgagga 7620
caacgagcag aagcagctgt tcgtcgagca gcacaagcat tacctcgacg agatcattga 7680
gcagatttcc gagttctcca agcgcgtgat cctggccgac gcgaatctgg ataaggtcct 7740
ctccgcgtac aacaagcacc gcgacaagcc aatcagggag caggctgaga atatcattca 7800
tctcttcacc ctgacgaacc tcggcgcccc tgctgctttc aagtacttcg acacaactat 7860
cgatcgcaag aggtacacaa gcactaagga ggtcctggac gcgaccctca tccaccagtc 7920
gattaccggc ctctacgaga cgcgcatcga cctgtctcag ctcgggggcg acaagcggcc 7980
agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag acccgcgact ccggcggcag 8040
cacgaacctc tccgacatca tcgagaagga gacgggcaag cagctcgtga tccaggagag 8100
catcctcatg ctgccggagg aggtggagga ggtcatcggc aacaagcccg agtccgacat 8160
cctcgtgcac accgcctacg acgagtccac ggacgagaac gtcatgctcc tgacgagcga 8220
cgctccagag tacaagccat gggctctcgt gatccaggac agcaacggcg agaacaagat 8280
caagatgctg tccggcggct ccccgaagaa gaagcgcaag gtctgagctc agagctttcg 8340
ttcgtatcat cggtttcgac aacgttcgtc aagttcaatg catcagtttc attgcgcaca 8400
caccagaatc ctactgagtt tgagtattat ggcattggga aaactgtttt tcttgtacca 8460
tttgttgtgc ttgtaattta ctgtgttttt tattcggttt tcgctatcga actgtgaaat 8520
ggaaatggat ggagaagagt taatgaatga tatggtcctt ttgttcattc tcaaattaat 8580
attatttgtt ttttctctta tttgttgtgt gttgaatttg aaattataag agatatgcaa 8640
acattttgtt ttgagtaaaa atgtgtcaaa tcgtggcctc taatgaccga agttaatatg 8700
aggagtaaaa cacttgtagt tgtaccatta tgcttattca ctaggcaaca aatatatttt 8760
cagacctaga aaagctgcaa atgttactga atacaagtat gtcctcttgt gttttagaca 8820
tttatgaact ttcctttatg taattttcca gaatccttgt cagattctaa tcattgcttt 8880
ataattatag ttatactcat ggatttgtag ttgagtatga aaatattttt taatgcattt 8940
tatgacttgc caattgattg acaacgaatt cgtaatcatg tcatagctgt ttcctgtgtg 9000
aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc 9060
ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt 9120
ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg 9180
cggtttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac 9240
tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa 9300
agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa 9360
aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct 9420
atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc 9480
atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgataac 9540
atggtggagc acgacactct cgtctactcc aagaatatca aagatacagt ctcagaagac 9600
caaagggcta ttgagacttt tcaacaaagg gtaatatcgg gaaacctcct cggattccat 9660
tgcccagcta tctgtcactt catcaaaagg acagtagaaa aggaaggtgg cacctacaaa 9720
tgccatcatt gcgataaagg aaaggctatc gttcaagatg cctctgccga cagtggtccc 9780
aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 9840
tcaaagcaag tggattgatg tgatatctcc actgacgtaa gggatgacgc acaatcccac 9900
tatccttcgc aagaccttcc tctatataag gaagttcatt tcatttggag aggacacgct 9960
gaaatcacca gtctctctct acaaatctat ctctctcgag ctttcgcaga tcccgggggg 10020
caatgagata tgaaaaagcc tgaactcacc gcgacgtctg tcgagaagtt tctgatcgaa 10080
aagttcgaca gcgtctccga cctgatgcag ctctcggagg gcgaagaatc tcgtgctttc 10140
agcttcgatg taggagggcg tggatatgtc ctgcgggtaa atagctgcgc cgatggtttc 10200
tacaaagatc gttatgttta tcggcacttt gcatcggccg cgctcccgat tccggaagtg 10260
cttgacattg gggagtttag cgagagcctg acctattgca tctcccgccg tgcacagggt 10320
gtcacgttgc aagacctgcc tgaaaccgaa ctgcccgctg ttctacaacc ggtcgcggag 10380
gctatggatg cgatcgctgc ggccgatctt agccagacga gcgggttcgg cccattcgga 10440
ccgcaaggaa tcggtcaata cactacatgg cgtgatttca tatgcgcgat tgctgatccc 10500
catgtgtatc actggcaaac tgtgatggac gacaccgtca gtgcgtccgt cgcgcaggct 10560
ctcgatgagc tgatgctttg ggccgaggac tgccccgaag tccggcacct cgtgcacgcg 10620
gatttcggct ccaacaatgt cctgacggac aatggccgca taacagcggt cattgactgg 10680
agcgaggcga tgttcgggga ttcccaatac gaggtcgcca acatcttctt ctggaggccg 10740
tggttggctt gtatggagca gcagacgcgc tacttcgagc ggaggcatcc ggagcttgca 10800
ggatcgccac gactccgggc gtatatgctc cgcattggtc ttgaccaact ctatcagagc 10860
ttggttgacg gcaatttcga tgatgcagct tgggcgcagg gtcgatgcga cgcaatcgtc 10920
cgatccggag ccgggactgt cgggcgtaca caaatcgccc gcagaagcgc ggccgtctgg 10980
accgatggct gtgtagaagt actcgccgat agtggaaacc gacgccccag cactcgtccg 11040
agggcaaaga aatagagtag atgccgaccg gatctgtcga tcgacaagct cgagtttctc 11100
cataataatg tgtgagtagt tcccagataa gggaattagg gttcctatag ggtttcgctc 11160
atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat acttctatca 11220
ataaaatttc taattcctaa aaccaaaatc cagtactaaa atccagatcc cccgaattaa 11280
ttcggcgtta attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt 11340
caatttgttt acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca 11400
gctcggcaca aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg 11460
gagagccgtt gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc 11520
aactaagctg ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa 11580
cgatgacaga gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact 11640
atgttatacg ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt 11700
tagaatgcaa ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc 11760
tttaaatact gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg 11820
aattgaaaaa actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg 11880
ctaaggtata taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc 11940
ggtataaagg gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag 12000
gaaagctgcc tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc 12060
tcatgagtga ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg 12120
aaaagattat cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg 12180
attgtcccta tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata 12240
acgatctggc cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc 12300
gcgagctgta tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg 12360
gcgacctggg agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc 12420
ttgggagaag cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca 12480
gggaggatat cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc 12540
ctgattggga gaaaataaaa tattatattt tactggatga attgttttag tacctagaat 12600
gcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 12660
agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 12720
aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 12780
cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 12840
agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 12900
tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 12960
gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 13020
gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 13080
ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 13140
gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 13200
ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 13260
ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 13320
acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 13380
gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 13440
cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 13500
tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc 13560
gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc 13620
gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg 13680
gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt 13740
gatgtgggcg ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc 13800
ctggccgtag gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg 13860
cggggcgtag ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tcggctgtgc 13920
gctggccaga cagttatgca caggccaggc gggttttaag agttttaata agttttaaag 13980
agttttaggc ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc 14040
ggttcccaat gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct 14100
ttgggttccc aatgtacgtg ctatccacag gaaacagacc ttttcgacct ttttcccctg 14160
ctagggcaat ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct 14220
cgatcaggtt gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca 14280
aatcgtactc cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct 14340
tgaactctcc ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg 14400
ccttgcctgc ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca 14460
aaaagtaatc ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt 14520
acatccaatc agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga 14580
tcttgtagcg gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg 14640
ccttcttcgt acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca 14700
ggtcgtcttt ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt 14760
gtggacggaa cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt 14820
cggttagatg ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg 14880
ccggccctgc ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag 14940
ctcgtcggtc acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc 15000
gggtgcccac gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg 15060
gcttcctaat cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat 15120
cagcggccgc ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg 15180
cggcctgcgc ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta 15240
ccgggccgga tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc 15300
attgcagggc cggcagacaa cccagccgct tacgcctggc caaccgcccg ttcctccaca 15360
catggggcat tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt 15420
agccgctaaa attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga 15480
tgtattcaga tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct 15540
tggtgtgatc ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca 15600
ggctggccaa cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt 15660
ttgtgctttt gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc 15720
agcggccagc gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt 15780
tgtgccggcg gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat 15840
gggcagctcg tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat 15900
cgcccgcgac acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt 15960
aaccagctcc accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat 16020
cagcacgaag tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc 16080
gtcgatcact acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg 16140
gtcgatgccg acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact 16200
gccctgggga tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc 16260
tagatgggtt gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac 16320
cttcatgcgt tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc 16380
gcatgacgca agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt 16440
tcttcagcgg ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg 16500
atttcatgca gccgcacggt tgagacgtgc gcgggcggct cgaacacgta cccggccgcg 16560
atcatctccg cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc 16620
ggtttcatgc ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg 16680
tcaatgcgtc ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc 16740
tgcgctcaag tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt 16800
tcacggtgcg gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga 16860
gggtagggcg ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg 16920
tgcggtcgat gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca 16980
tgcggccggc cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc 17040
cggcctcctg gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt 17100
ctagcctggt cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct 17160
ccgggcggtc gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg 17220
cgtgcagttc ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc 17280
ccagcaggcc agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag 17340
tattctactt tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg 17400
cagcctgtcg cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg 17460
aacgtcagaa gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc 17520
gaggggaacc ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg 17580
cccttttaaa tatccgttat tctaa 17605
<210> 172
<211> 1785
<212> DNA
<213> Oryza sativa
<400> 172
atgcagtcgc ttgccctcac ctccccctcc cttccggcgc cggcgaccgc atccggccgc 60
cgccgccgcc gcctggagcg catccgcgcc accgcggtgt ccgacgagcc gaagctgaac 120
aagtacagcg cgcgcatcac ggagcccaag tcgcaggggg cgtcgcaggc ggtgctctac 180
ggcgtggggc tcaccgacgc cgacctccgc aagccgcagg tgggcgtctc ctccgtctgg 240
tacgagggca acacctgcaa catgcacctt ctccgcctcg ccgaggccgt cagggacggc 300
gtccgcgagg ccggcatggt ggccttccgc ttcaacaccg tcggggtcag cgacgccatc 360
tccatgggca cccggggcat gtgctacagc ctccagtcgc gcgacctcat cgccgacagc 420
atcgagaccg tcatgggcgc gcagcactac gacgccaaca tctccattcc tggatgcgac 480
aagaacatgc caggaacaat aatggcaatg gggcggctga accgaccaag tatcatgata 540
tatggtggaa ctattaagcc tggtcatttt cagggtaatt cttatgacat agtatctgct 600
ttccagtgct atggagaatt tgttaccgga tcaatcagtg atgagcaaag aaagaatgtg 660
ctccgcaact catgcccagg agcaggtgcg tgtggtggta tgtacaccgc aaatacaatg 720
gcatccgcca tcgagaccat gggtatgagc cttccgtaca gttcttcaac ccctgctgaa 780
gacccactaa agctagagga atgccgtctt gctgggaagt atcttttaga attgttaaag 840
atggatttga agcctaaaga cattatcact gagaaatctt tgcggaacgc aatggtcatt 900
gtcatggcac ttggtgggtc tactaatgct gttttgcatt taattgctat cgctaggtct 960
gtaggtctgc aattaactct tgatgatttt cagaaggtca gtgaccaagt tcctttcctt 1020
gcggacctta agcctagtgg caaatatgtc atggaggatc tgcataagat tggtggaaca 1080
cctgcagtaa ttcattatct tctggagcaa ggtcttcttg atggtgattg tatgaccgtc 1140
actgggaaaa ctctagcgga aaatgctaaa atcttccctc ctctgtccga aggacagcaa 1200
ataataaggc ccctggacaa cccaatcaaa tcaactggtc atatacaaat actttatggc 1260
aatcttgcac cagaaggttc tgtagcaaaa attactggca aagaggggat gtttttctca 1320
ggccctgcac tagtgtttga gggtgaagaa tctatgatta cagctatatc agaaaaccca 1380
gcgaatttca aggggaaggt agtagtgata cgaggagaag gaccgaaagg tggaccaggg 1440
atgcctgaaa tgctgactcc aactagtgca ataatgggtg ctggtcttgg gaaggagtgt 1500
gccctgctga ccgatggcag attttctgga ggatcacatg gatttgttgt aggccacatt 1560
tgtcctgaag cacaggaggg aggtccaatt ggtcttgttc aaaacggtga caaaattacc 1620
attgatgttg tgaagagagt aattgatgtt gaactgactg aagctgagct ggaagaaaga 1680
cggaggaaat ggacgccgcc gccacacaag gctgcccgtg gagcgctttg gaagtacacg 1740
aagctcgtgt cccctgcatc aagaggatgc gtcaccgatg agtag 1785
<210> 173
<211> 3676
<212> DNA
<213> Triticum aestivum
<400> 173
gcggctccgg gtagccgttc actttgatcg ccagtagttt cttgccccgc tgcttttctc 60
gggggtttct tgcacttctt gcggttcgtg tttggtgggg cgtcacctca atcccgcgcg 120
gcttcttgct gacgcgtttc tcggcttctc tgctccgagt ccctcctaga ttccccgcct 180
ggttaatgag cggactcctc ctcttctttt ccccaaattt ttaccctggg ccagttagaa 240
atttttagtc agagctctct taaggagatt cttggtcaag tacaagtttt tggttagcct 300
gggatcttgc ctgagaaatg tgtatggggt tttttgctgc cgtttttgcg gccttaatgg 360
catcaatgat ctggaagaaa tcagtgaacg cgcttgctca agtttgcccc ctttttttct 420
ctgtagaaac atctttagag aacgactggt ttggtatgga atcggtactt ggttaagttt 480
ggtgcaagat taatgctcgc tttacctttg cgttactttg atttcttcga tctgtgagat 540
tttaccagtc ttggtaaatg tttcaccacc tctgattagt cttgatttcc agtagtatat 600
tttttttgcg tgcttctgta ataatactag tgttgatatc ctagtggctg ttgactttgg 660
ttcttggcaa gtacagtaaa tgttttgatt ctccaaggct gaatgctgat ctttaccttg 720
ctctgcagtt ttgtgtcctg agccatggct gccaagctga ctcgcctcca cagcctcagg 780
gagcgccttg gtgccacctt ctcctcccac cccaatgagc tcattgcact cttctccagg 840
tatgttcacc agggcaaagg catgctccag cgccaccagc tgcttgctga gtttgacgaa 900
ctgttcgaat ccgacaagga gaagtatgcg ccctttgaag acatcctccg agctgctcag 960
gaagcaattg tgctgccccc atgggttgca cttgccatca ggcccaggcc tggtgtctgg 1020
gactacattc gggtgaatgt tagtgagctc gctgttgaag agctcactgt ttctgagtac 1080
ttggcattca aggaacagct tgtcgatgag catgccagca gcaagtttgt gcttgagctt 1140
gattttgagc ctttcaatgc ctccttcccg cgcccttcca tgtccaagtc catcggaaac 1200
ggggtgcagt tccttaaccg tcacctgtct tccaagttgt tccaggacaa ggagagcctc 1260
taccctctgc tcaacttcct gaaagcccat aactacaagg gcacgacaat gatgttgaac 1320
gacagaattc agagccttcg cggtctccag tcagccctta ggaaggcaga agagtatcta 1380
gtcagcattc ctgaagacac tcccagctct gaattcaacc acaggttcca agagcttggc 1440
ttggagaagg gttggggtga caccgcaaag cgtgtacacg acaccatcca tttgcttctg 1500
gaccttcttg aggcccctga tccggccagc ttggagaagt tccttggaac cattccaatg 1560
atgttcaatg ttgtcatcct gtctccccat ggatactttg ctcaatccaa tgtgttggga 1620
taccctgata ccggtggcca ggttgtgtac atcttggatc aagtccgtgc tttggagaat 1680
gagatgcttc tgaggattaa gcagcaaggc cttgatataa ctcccaagat cctcattgta 1740
accaggttgt tgcctgatgc tgttggaacc acatgtggcc agcggctgga gaaggtcatt 1800
ggaactgagc acactgacat tctccgtgtt ccattcagaa ctgagaatgg gatccttcgt 1860
aagtggatct cgcgttttga tgtctggcca tacctggaga catacaccga ggatgttgca 1920
aacgaactca tgagagaaat gcagaccaag cctgatttga tcattggcaa ctacagtgat 1980
ggtaaccttg tggccactct gcttgcccat aaattgggag ttacccagtg caccattgcc 2040
catgccttgg agaaaaccaa gtaccccaac tcagacatct acttggacaa attcgacagc 2100
cagtatcact tttcatgcca gttcacagct gacctgattg ccatgaacca cactgatttc 2160
atcatcacca gcacattcca ggaaattgcc ggaagcaagg atagcgtggg ccaatacgag 2220
tctcacattg ctttcaccct ccctgatctg taccgggttg tccatgggat tgacgtgttt 2280
gatcctaagt tcaacatcgt ctctcctgga gcagacatga ctgtctactt cccatacact 2340
gagactgaca agaggctcac cgccttccac tctgaaattg aggagctcct gtacagcgat 2400
gttgagaacg atgaacacaa atttgtgttg aaggacagga acaagccaat catcttttca 2460
atggctcgtc ttgaccgtgt gaagaacatg actggcttgg ttgagatgta cggcaagaat 2520
gcacatctga aggatttggc gaaccttgtg attgttgctg gcgaccatgg caaggagtcc 2580
aaggataggg aggagcaggc tgagttcaag aggatgtaca gtctcattga ggagtacaag 2640
ctgaagggcc atatccgttg gatctctgct cagatgaacc gtgttcgcaa tggtgagctg 2700
taccgctaca tctgtgacac caagggagca tttgtgcagc ctgcattcta tgaagctttt 2760
ggccttactg tcattgaggc catgacatgt ggtctgccga caattgcgac atgccacggt 2820
ggccctgctg aaatcattgt gaatggggtg tctggtgtgc acattgatcc ttaccacagt 2880
gacaaggccg cagatatctt ggtcaacttc tttgagaagt gcagcgagga tccaagctac 2940
tgggacaaaa tgtctgaagg aggcctgaag agaatttatg agaagtacac ctggaagctg 3000
tactcagaga ggctgatgac cctgaccggt gtgtatggat tctggaagta cgtgagcaac 3060
ctagagaggc gtgagactcg ccgttacctt gagatgttct acgccctgaa gtaccgcagc 3120
ctggctgctg cagttccatt ggcggttgac ggcgagaact ccgacaacta gcccggtgca 3180
atggggcatg aagaggcgca ttcagcggga gggtgaagtg tcggctgcgt tatgatttgt 3240
ctgccgtagt tttcatttgg tctggtcgtg gagtgtgtta atttgttgtc tcggcacatt 3300
tgtgaggtcc tgggcagtgc tcgctggttc atgccagccg catttgttgc ctggaataaa 3360
cgcctgccca tggtgttcca tctttcatca gagttcaatg caatgtttcg ttgtctgtct 3420
catttctctg tctttcaaaa ttatttgatc accccttttg tcatgaatga acctggtgtg 3480
tgtgtggttt tggtcaaagc ttcactgatg tgtgctgcac tgtggagttt ttctgaagaa 3540
tagtgccggt cttggcaagg acaaaaccaa atgccaacaa cctatgcact gtaacattgg 3600
ttatgtgatc aaagttctcc ggaaactaaa tactactttg tagtgtcaaa aacgctttta 3660
tattatggga tggagg 3676
<210> 174
<211> 3055
<212> DNA
<213> Solanum lycopersicum
<400> 174
tatatcaaaa aatatcgggc agttttgtag ttttaaacat attatataca atttaaattt 60
ttaaaaattg ctaaaaatag aagaaataaa gattattttt atgaaagttt ttttttaaaa 120
gattaatcag attgtgagtt tttctttttt tgacttattg atgtgatgtt tatattagag 180
aagccagcaa tgcaagtagc tgcaaaaggg tactaatata tatatatata tatatatata 240
tagcatcaat caaccataga tcttgatctg atgctaataa ttaattttat tattattttt 300
tatcgatatt catattgaga tttgaaaaaa taatctttca tatatgttca ttcaatgacg 360
gagaccaaat ttaaatcgag tggtgtcaaa attatacaac gtaatcaatt gtttaaaaat 420
gagaagaaga aaaaaacact aaagttaaaa cttgagctaa gatcgaaaaa ccgatcatta 480
agagttttta caactcgtta tcttgtgtca cgagatgtca atattaatat ctatcgaaaa 540
ataatcaaaa tttgatttat aattttccga taaacatata ttgttggaca cgtcatagac 600
aacatatgtt atcaataaaa gatcggataa ataaattaaa acgaaagaaa taaagaaaat 660
taatcatatc taaagtaatg attacacttt gtttaagata ttgtcagtaa tatatattta 720
ttagtacaac ttcaaatgtc aaaaatttat cctcaaattt tcctattggg ttgggcaggt 780
gtccttttgc atgaaataat ccctactaat atttacttgt gataagatat aggtgcagtt 840
attttcagat aataagccgt cactctgtct tgttactgca gtaaaaataa taataactat 900
tttcttcgtt ttaatttgtc tgatattatt cgatcaaatt taaagataag ttttataata 960
aggttgaata tatagtcagt agatcctact tataaacata caaatagagt ttacacacat 1020
caactttctc cagatcctac ttataaaatt atactgaata tatcgttatc ggttatcgat 1080
ttgacacaaa ttttaaacat tgaaaaggtg acaatgaaag agatccatag tacagtactt 1140
ggtgggaatc taattagcat aactaaaact gagagttgta tgggttttat atacagctac 1200
tactattata tgatcactac gtcacaatat cttaattatc attggtttat agtactttca 1260
ttatagaatc actaagtaca gtttgatgcc aaatataatc ctttttctaa ttttaattat 1320
ttttttttat acaatattgt tttctatagt gtctaattta accatttaat atttgaaatt 1380
caatatccta caacataatt tggtgtttcg tagctagtct taccagtatt ctcatagttt 1440
catgtgtttc gtttttctgt tacttacatg ttattttttg tgtttcgatt atcttgtatt 1500
atgttgatat ataaaactaa ttctgacttt agctaataag taactcgaac gaaaaaacaa 1560
tctatctaac cccataaagg caggggataa ggtctctcgc cctcctcaaa ctctacttga 1620
aaaattacac tgatgtattg tcgttgttgt tattgttaac tcaaactaaa cacaagataa 1680
atttaatctc attctttctt aaccaaagcc tcttaactat tttctttgag caaagtaaaa 1740
ttgaaagaat caaataaata actgagatga gctcatcttt atgtattgcc tcagcatgta 1800
gaggatttca gaactgcact gtcatatcat aaaaagtata cttatgatat acaacaatgg 1860
ctgcatggct catcattttc ttttgttcta taaattggct tttactatct tcacttctta 1920
tgcactcaac taacactgat ccattttgtt tcattacaca cttagatcaa gaacttgctt 1980
tttcatttgc ttcttgaaaa atgtctttga tcaatgctaa atatttcaat ctctttgtct 2040
tgctgatctg ttttttagta attcaagaat ctcatggtct gtctctaaag gaagttgctc 2100
ctgtgaaact cctaaacaga aaggttttta acttttcatc ttattctcat actatctttt 2160
actattgtct aattcattta tactatctat tcattcgtga gtctttactg ccctgttctt 2220
ttgttacttt ccttattttg atcatccacg aggttgtaga aagagagaaa gggggtgggg 2280
tggctatcta gaggagttgt ttcggttgta tgtcacgatg ggcaatggta caagtgaatc 2340
atcgcttaga tttccatgac taagtactaa tcctttaaaa ctatatgtga atgcaggttt 2400
tagaaaggca gtgggctgca tttgggaagg tatactacaa gcatgcagag aagattaatg 2460
agaagtttgc tgattgggag ctaagaggag ttccagctgg tcctgatcca ttgcatcaca 2520
atggtgctag tcctaagaaa cctaagactc cataatcatg caatattagt attgttttag 2580
ctctatgttc aagtttttag taaagggtag tatatggttt catctttctg tttttgttgt 2640
ttttggccca atagaaaagg aaaaaacaaa aagaaaacga aaatgtaagt atgggcttag 2700
gctttaggct agccaagttg gaatattagg atttcatgtc tccctgatat gtatatagct 2760
ttgatgcaaa gttttcgttt ttataactag tactaagaaa actcaagaat gaacacaagt 2820
ttttgctgta ctagcaatgt catcgatcca acgaacgttt ttgagaagag acaaagtcag 2880
ttgaggaaca acaacaacaa caacaaacac acattgtagt ccaacaagtg gggttgtgga 2940
gtttgggaag ggtagtatgt atgcaaactt atccctacct tgtgaatatt gagagattgt 3000
taacgaatca gttgaggaag aagagacaaa aaaaactcga gtccaagaca catgt 3055
<210> 175
<211> 6381
<212> DNA
<213> Artificial Sequence
<220>
<223> STEME-1
<400> 175
atggaggcca gcccggctag cggcccaagg catctcatgg acccgcacat cttcaccagc 60
aacttcaaca acggcatcgg caggcacaag acctacttgt gctacgaggt ggagaggctc 120
gacaacggaa cctccgtgaa gatggaccaa cacagggggt tcctccacaa ccaagccaag 180
aacctcctct gcggcttcta cggcaggcac gccgagttga ggttcctcga cttggtgcca 240
tccctccaac tcgatccagc ccaaatctac cgcgtgacct ggttcatctc ctggtcccca 300
tgcttctcct ggggttgcgc cggcgaggtt cgggctttcc tccaagaaaa cacccacgtc 360
cgcctccgca ttttcgccgc caggatctat gattacgacc ctctctacaa ggaggccctc 420
cagatgctgc gggacgccgg tgctcaggtg agtatcatga cctacgacga gttcaagcac 480
tgctgggaca ccttcgttga ccaccagggc tgcccattcc aaccatggga cggtctggat 540
gaacacagcc aagccttgtc cggcaggctc cgggccatcc tccaaaacca ggggaactcc 600
gggagcgaga cgccaggcac ctccgagtcg gccaccccag aatcttccgg cggatcttct 660
ggaggatcta gcggctccga gacaccagga acatccgaat ccgctacacc agagtcttct 720
ggaggatcta gcggaggatc ttccgaggtg gagttctctc acgagtattg gatgaggcac 780
gctcttacac ttgctaagag agcttgggac gaaagagaag tgccagttgg cgccgttctt 840
gtgcataata atagggtgat cggcgagggt tggaatagac caattggaag gcatgatcca 900
acagctcacg cagagattat ggctctcaga caaggcggcc tcgttatgca gaactacagg 960
ctcattgacg ctacactcta cgtgacactc gaaccttgcg ttatgtgcgc cggagctatg 1020
attcattcta ggattggcag ggtcgtgttt ggagctaggg acgctaaaac aggagccgcc 1080
ggatctctta tggacgtgtt gcatcatcca ggcatgaacc atagggtgga gattacagag 1140
ggcattcttg cagacgagtg cgctgctctt ctttccgatt tcttcaggat gagaaggcag 1200
gagattaagg cccagaagaa ggctcagtct tctacagata gcggaggatc ttccggagga 1260
tctagcggct ccgagacacc aggaacatcc gaaagcgcta caccagaatc tagcggaggc 1320
tcttccggag gatcttctga agtggagttc tcccacgagt attggatgag gcacgctctt 1380
acacttgcta aaagggctag ggacgaaagg gaagttccag ttggagctgt tctcgtgctc 1440
aataacaggg tgattggcga gggttggaat agagccattg gactccatga tccaacagct 1500
cacgcagaga ttatggctct tagacaaggc ggcctcgtta tgcagaatta cagactcatc 1560
gacgccacac tctacgttac cttcgaacct tgcgttatgt gcgccggagc tatgatccat 1620
tctaggattg gcagggtcgt gttcggcgtt agaaacgcta agacaggagc tgcaggctct 1680
cttatggacg ttcttcatta cccaggcatg aatcatagag tggagatcac agaaggcatt 1740
cttgcagacg agtgcgcagc tctcctttgc tatttcttca ggatgccgag gcaagttttc 1800
aacgctcaga agaaggccca gtcttctaca gattccggcg gatcttctgg aggatctagc 1860
ggctccgaga caccaggaac atccgaatcc gctacaccag agtcttctgg aggatctagc 1920
ggaggatctg acaagaagta ctcgatcggc ctcgccattg ggactaactc tgttggctgg 1980
gccgtgatca ccgacgagta caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc 2040
gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc tgttcgacag cggcgagacg 2100
gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc 2160
atctgctacc tgcaggagat tttctccaac gagatggcga aggttgacga ttctttcttc 2220
cacaggctgg aggagtcatt cctcgtggag gaggataaga agcacgagcg gcatccaatc 2280
ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt accctacgat ctaccatctg 2340
cggaagaagc tcgtggactc cacagataag gcggacctcc gcctgatcta cctcgctctg 2400
gcccacatga ttaagttcag gggccatttc ctgatcgagg gggatctcaa cccggacaat 2460
agcgatgttg acaagctgtt catccagctc gtgcagacgt acaaccagct cttcgaggag 2520
aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag 2580
tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg agaagaagaa cggcctgttc 2640
gggaatctca ttgcgctcag cctggggctc acgcccaact tcaagtcgaa tttcgatctc 2700
gctgaggacg ccaagctgca gctctccaag gacacatacg acgatgacct ggataacctc 2760
ctggcccaga tcggcgatca gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac 2820
gccatcctcc tgtctgatat tctcagggtg aacaccgaga ttacgaaggc tccgctctca 2880
gcctccatga tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg 2940
gtcaggcagc agctccccga gaagtacaag gagatcttct tcgatcagtc gaagaacggc 3000
tacgctgggt acattgacgg cggggcctct caggaggagt tctacaagtt catcaagccg 3060
attctggaga agatggacgg cacggaggag ctgctggtga agctcaatcg cgaggacctc 3120
ctgaggaagc agcggacatt cgataacggc agcatcccac accagattca tctcggggag 3180
ctgcacgcta tcctgaggag gcaggaggac ttctaccctt tcctcaagga taaccgcgag 3240
aagatcgaga agattctgac tttcaggatc ccgtactacg tcggcccact cgctaggggc 3300
aactcccgct tcgcttggat gacccgcaag tcagaggaga cgatcacgcc gtggaacttc 3360
gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca tcgagaggat gacgaatttc 3420
gacaagaacc tgccaaatga gaaggtgctc cctaagcact cgctcctgta cgagtacttc 3480
acagtctaca acgagctgac taaggtgaag tatgtgaccg agggcatgag gaagccggct 3540
ttcctgtctg gggagcagaa gaaggccatc gtggacctcc tgttcaagac caaccggaag 3600
gtcacggtta agcagctcaa ggaggactac ttcaagaaga ttgagtgctt cgattcggtc 3660
gagatctctg gcgttgagga ccgcttcaac gcctccctgg ggacctacca cgatctcctg 3720
aagatcatta aggataagga cttcctggac aacgaggaga atgaggatat cctcgaggac 3780
attgtgctga cactcactct gttcgaggac cgggagatga tcgaggagcg cctgaagact 3840
tacgcccatc tcttcgatga caaggtcatg aagcagctca agaggaggag gtacaccggc 3900
tgggggaggc tgagcaggaa gctcatcaac ggcattcggg acaagcagtc cgggaagacg 3960
atcctcgact tcctgaagag cgatggcttc gcgaaccgca atttcatgca gctgattcac 4020
gatgacagcc tcacattcaa ggaggatatc cagaaggctc aggtgagcgg ccagggggac 4080
tcgctgcacg agcatatcgc gaacctcgct ggctcgccag ctatcaagaa ggggattctg 4140
cagaccgtga aggttgtgga cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac 4200
atcgtcattg agatggcccg ggagaatcag accacgcaga agggccagaa gaactcacgc 4260
gagaggatga agaggatcga ggagggcatt aaggagctgg ggtcccagat cctcaaggag 4320
cacccggtgg agaacacgca gctgcagaat gagaagctct acctgtacta cctccagaat 4380
ggccgcgata tgtatgtgga ccaggagctg gatattaaca ggctcagcga ttacgacgtc 4440
gatcatatcg ttccacagtc attcctgaag gatgactcca ttgacaacaa ggtcctcacc 4500
aggtcggaca agaaccgggg caagtctgat aatgttcctt cagaggaggt cgttaagaag 4560
atgaagaact actggcgcca gctcctgaat gccaagctga tcacgcagcg gaagttcgat 4620
aacctcacaa aggctgagag gggcgggctc tctgagctgg acaaggcggg cttcatcaag 4680
aggcagctgg tcgagacacg gcagatcact aagcacgttg cgcagattct cgactcacgg 4740
atgaacacta agtacgatga gaatgacaag ctgatccgcg aggtgaaggt catcaccctg 4800
aagtcaaagc tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgggagatc 4860
aacaattacc accatgccca tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc 4920
aagaagtacc caaagctcga gagcgagttc gtgtacgggg actacaaggt ttacgatgtg 4980
aggaagatga tcgccaagtc ggagcaggag attggcaagg ctaccgccaa gtacttcttc 5040
tactctaaca ttatgaattt cttcaagaca gagatcactc tggccaatgg cgagatccgg 5100
aagcgccccc tcatcgagac gaacggcgag acgggggaga tcgtgtggga caagggcagg 5160
gatttcgcga ccgtcaggaa ggttctctcc atgccacaag tgaatatcgt caagaagaca 5220
gaggtccaga ctggcgggtt ctctaaggag tcaattctgc ctaagcggaa cagcgacaag 5280
ctcatcgccc gcaagaagga ctgggatccg aagaagtacg gcgggttcga cagccccact 5340
gtggcctact cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag 5400
agcgtgaagg agctgctggg gatcacgatt atggagcgct ccagcttcga gaagaacccg 5460
atcgatttcc tggaggcgaa gggctacaag gaggtgaaga aggacctgat cattaagctc 5520
cccaagtact cactcttcga gctggagaac ggcaggaagc ggatgctggc ttccgctggc 5580
gagctgcaga aggggaacga gctggctctg ccgtccaagt atgtgaactt cctctacctg 5640
gcctcccact acgagaagct caagggcagc cccgaggaca acgagcagaa gcagctgttc 5700
gtcgagcagc acaagcatta cctcgacgag atcattgagc agatttccga gttctccaag 5760
cgcgtgatcc tggccgacgc gaatctggat aaggtcctct ccgcgtacaa caagcaccgc 5820
gacaagccaa tcagggagca ggctgagaat atcattcatc tcttcaccct gacgaacctc 5880
ggcgcccctg ctgctttcaa gtacttcgac acaactatcg atcgcaagag gtacacaagc 5940
actaaggagg tcctggacgc gaccctcatc caccagtcga ttaccggcct ctacgagacg 6000
cgcatcgacc tgtctcagct cgggggcgac aagcggccag cggcgacgaa gaaggcgggg 6060
caggcgaaga agaagaagga attctccggc ggcagcacca acctgtccga catcatcgag 6120
aaggagacgg gcaagcaact cgtgatccag gagagcatcc tcatgctgcc agaggaggtg 6180
gaggaggtca tcggcaacaa gccagagtcc gacatcctgg tgcacaccgc ctacgacgag 6240
tccaccgacg agaacgtcat gctcctgacc agcgacgccc cagagtacaa gccatgggcc 6300
ctcgtcatcc aggacagcaa cggggagaac aagatcaaga tgctgtcggg ggggagccca 6360
aagaagaagc ggaaggtgta g 6381
<210> 176
<211> 2126
<212> PRT
<213> Artificial Sequence
<220>
<223> STEME-1
<400> 176
Met Glu Ala Ser Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His
1 5 10 15
Ile Phe Thr Ser Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr
20 25 30
Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met
35 40 45
Asp Gln His Arg Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys
50 55 60
Gly Phe Tyr Gly Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro
65 70 75 80
Ser Leu Gln Leu Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile
85 90 95
Ser Trp Ser Pro Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala
100 105 110
Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg
115 120 125
Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg
130 135 140
Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His
145 150 155 160
Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp
165 170 175
Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala
180 185 190
Ile Leu Gln Asn Gln Gly Asn Ser Gly Ser Glu Thr Pro Gly Thr Ser
195 200 205
Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser
210 215 220
Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser
225 230 235 240
Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr
245 250 255
Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg
260 265 270
Glu Val Pro Val Gly Ala Val Leu Val His Asn Asn Arg Val Ile Gly
275 280 285
Glu Gly Trp Asn Arg Pro Ile Gly Arg His Asp Pro Thr Ala His Ala
290 295 300
Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg
305 310 315 320
Leu Ile Asp Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys Val Met Cys
325 330 335
Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Ala
340 345 350
Arg Asp Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His
355 360 365
His Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala
370 375 380
Asp Glu Cys Ala Ala Leu Leu Ser Asp Phe Phe Arg Met Arg Arg Gln
385 390 395 400
Glu Ile Lys Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly
405 410 415
Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser
420 425 430
Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Glu Val
435 440 445
Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys
450 455 460
Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu
465 470 475 480
Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His
485 490 495
Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu
500 505 510
Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe
515 520 525
Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly
530 535 540
Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser
545 550 555 560
Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile
565 570 575
Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe
580 585 590
Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser
595 600 605
Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr
610 615 620
Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser
625 630 635 640
Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn
645 650 655
Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys
660 665 670
Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn
675 680 685
Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr
690 695 700
Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg
705 710 715 720
Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp
725 730 735
Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp
740 745 750
Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val
755 760 765
Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu
770 775 780
Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu
785 790 795 800
Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu
805 810 815
Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln
820 825 830
Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val
835 840 845
Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu
850 855 860
Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe
865 870 875 880
Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser
885 890 895
Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr
900 905 910
Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr
915 920 925
Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu
930 935 940
Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser
945 950 955 960
Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu
965 970 975
Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile
980 985 990
Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly
995 1000 1005
Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu
1010 1015 1020
Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
1025 1030 1035
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro
1040 1045 1050
His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln
1055 1060 1065
Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu
1070 1075 1080
Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala
1085 1090 1095
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu
1100 1105 1110
Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala
1115 1120 1125
Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn
1130 1135 1140
Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu
1145 1150 1155
Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr
1160 1165 1170
Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys
1175 1180 1185
Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val
1190 1195 1200
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp
1205 1210 1215
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu
1220 1225 1230
Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe
1235 1240 1245
Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu
1250 1255 1260
Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
1265 1270 1275
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu
1280 1285 1290
Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu
1295 1300 1305
Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp
1310 1315 1320
Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu
1325 1330 1335
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala
1340 1345 1350
Gln Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn
1355 1360 1365
Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val
1370 1375 1380
Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro
1385 1390 1395
Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln
1400 1405 1410
Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu
1415 1420 1425
Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val
1430 1435 1440
Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu
1445 1450 1455
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn
1460 1465 1470
Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe
1475 1480 1485
Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp
1490 1495 1500
Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
1505 1510 1515
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu
1520 1525 1530
Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly
1535 1540 1545
Gly Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu
1550 1555 1560
Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp
1565 1570 1575
Ser Arg Met Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg
1580 1585 1590
Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe
1595 1600 1605
Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile Asn Asn Tyr
1610 1615 1620
His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly Thr Ala
1625 1630 1635
Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr Gly
1640 1645 1650
Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys Ser Glu
1655 1660 1665
Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn
1670 1675 1680
Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu
1685 1690 1695
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu
1700 1705 1710
Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val
1715 1720 1725
Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln
1730 1735 1740
Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
1745 1750 1755
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr
1760 1765 1770
Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val
1775 1780 1785
Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys
1790 1795 1800
Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys
1805 1810 1815
Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys
1820 1825 1830
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu
1835 1840 1845
Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala Gly Glu Leu Gln
1850 1855 1860
Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu
1865 1870 1875
Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp
1880 1885 1890
Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His Tyr Leu
1895 1900 1905
Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile
1910 1915 1920
Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys
1925 1930 1935
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His
1940 1945 1950
Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr
1955 1960 1965
Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu
1970 1975 1980
Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
1985 1990 1995
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro
2000 2005 2010
Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys Glu Phe
2015 2020 2025
Ser Gly Gly Ser Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr
2030 2035 2040
Gly Lys Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu
2045 2050 2055
Glu Val Glu Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu
2060 2065 2070
Val His Thr Ala Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu
2075 2080 2085
Leu Thr Ser Asp Ala Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile
2090 2095 2100
Gln Asp Ser Asn Gly Glu Asn Lys Ile Lys Met Leu Ser Gly Gly
2105 2110 2115
Ser Pro Lys Lys Lys Arg Lys Val
2120 2125
<210> 177
<211> 6291
<212> DNA
<213> Artificial Sequence
<220>
<223> STEME-2
<400> 177
atgtccgagg tggagttctc tcacgagtat tggatgaggc acgctcttac acttgctaag 60
agagcttggg acgaaagaga agtgccagtt ggcgccgttc ttgtgcataa taatagggtg 120
atcggcgagg gttggaatag accaattgga aggcatgatc caacagctca cgcagagatt 180
atggctctca gacaaggcgg cctcgttatg cagaactaca ggctcattga cgctacactc 240
tacgtgacac tcgaaccttg cgttatgtgc gccggagcta tgattcattc taggattggc 300
agggtcgtgt ttggagctag ggacgctaaa acaggagccg ccggatctct tatggacgtg 360
ttgcatcatc caggcatgaa ccatagggtg gagattacag agggcattct tgcagacgag 420
tgcgctgctc ttctttccga tttcttcagg atgagaaggc aggagattaa ggcccagaag 480
aaggctcagt cttctacaga tagcggagga tcttccggag gatctagcgg ctccgagaca 540
ccaggaacat ccgaaagcgc tacaccagaa tctagcggag gctcttccgg aggatcttct 600
gaagtggagt tctcccacga gtattggatg aggcacgctc ttacacttgc taaaagggct 660
agggacgaaa gggaagttcc agttggagct gttctcgtgc tcaataacag ggtgattggc 720
gagggttgga atagagccat tggactccat gatccaacag ctcacgcaga gattatggct 780
cttagacaag gcggcctcgt tatgcagaat tacagactca tcgacgccac actctacgtt 840
accttcgaac cttgcgttat gtgcgccgga gctatgatcc attctaggat tggcagggtc 900
gtgttcggcg ttagaaacgc taagacagga gctgcaggct ctcttatgga cgttcttcat 960
tacccaggca tgaatcatag agtggagatc acagaaggca ttcttgcaga cgagtgcgca 1020
gctctccttt gctatttctt caggatgccg aggcaagttt tcaacgctca gaagaaggcc 1080
cagtcttcta cagattccgg cggatcttct ggaggatcta gcggctccga gacaccagga 1140
acatccgaat ccgctacacc agagtcttct ggaggatcta gcggaggatc tgaggccagc 1200
ccggctagcg gcccaaggca tctcatggac ccgcacatct tcaccagcaa cttcaacaac 1260
ggcatcggca ggcacaagac ctacttgtgc tacgaggtgg agaggctcga caacggaacc 1320
tccgtgaaga tggaccaaca cagggggttc ctccacaacc aagccaagaa cctcctctgc 1380
ggcttctacg gcaggcacgc cgagttgagg ttcctcgact tggtgccatc cctccaactc 1440
gatccagccc aaatctaccg cgtgacctgg ttcatctcct ggtccccatg cttctcctgg 1500
ggttgcgccg gcgaggttcg ggctttcctc caagaaaaca cccacgtccg cctccgcatt 1560
ttcgccgcca ggatctatga ttacgaccct ctctacaagg aggccctcca gatgctgcgg 1620
gacgccggtg ctcaggtgag tatcatgacc tacgacgagt tcaagcactg ctgggacacc 1680
ttcgttgacc accagggctg cccattccaa ccatgggacg gtctggatga acacagccaa 1740
gccttgtccg gcaggctccg ggccatcctc caaaaccagg ggaactccgg gagcgagacg 1800
ccaggcacct ccgagtcggc caccccagaa tctcttaagg acaagaagta ctcgatcggc 1860
ctcgccattg ggactaactc tgttggctgg gccgtgatca ccgacgagta caaggtgccc 1920
tcaaagaagt tcaaggtcct gggcaacacc gatcggcatt ccatcaagaa gaatctcatt 1980
ggcgctctcc tgttcgacag cggcgagacg gctgaggcta cgcggctcaa gcgcaccgcc 2040
cgcaggcggt acacgcgcag gaagaatcgc atctgctacc tgcaggagat tttctccaac 2100
gagatggcga aggttgacga ttctttcttc cacaggctgg aggagtcatt cctcgtggag 2160
gaggataaga agcacgagcg gcatccaatc ttcggcaaca ttgtcgacga ggttgcctac 2220
cacgagaagt accctacgat ctaccatctg cggaagaagc tcgtggactc cacagataag 2280
gcggacctcc gcctgatcta cctcgctctg gcccacatga ttaagttcag gggccatttc 2340
ctgatcgagg gggatctcaa cccggacaat agcgatgttg acaagctgtt catccagctc 2400
gtgcagacgt acaaccagct cttcgaggag aaccccatta atgcgtcagg cgtcgacgcg 2460
aaggctatcc tgtccgctag gctctcgaag tctcggcgcc tcgagaacct gatcgcccag 2520
ctgccgggcg agaagaagaa cggcctgttc gggaatctca ttgcgctcag cctggggctc 2580
acgcccaact tcaagtcgaa tttcgatctc gctgaggacg ccaagctgca gctctccaag 2640
gacacatacg acgatgacct ggataacctc ctggcccaga tcggcgatca gtacgcggac 2700
ctgttcctcg ctgccaagaa tctgtcggac gccatcctcc tgtctgatat tctcagggtg 2760
aacaccgaga ttacgaaggc tccgctctca gcctccatga tcaagcgcta cgacgagcac 2820
catcaggatc tgaccctcct gaaggcgctg gtcaggcagc agctccccga gaagtacaag 2880
gagatcttct tcgatcagtc gaagaacggc tacgctgggt acattgacgg cggggcctct 2940
caggaggagt tctacaagtt catcaagccg attctggaga agatggacgg cacggaggag 3000
ctgctggtga agctcaatcg cgaggacctc ctgaggaagc agcggacatt cgataacggc 3060
agcatcccac accagattca tctcggggag ctgcacgcta tcctgaggag gcaggaggac 3120
ttctaccctt tcctcaagga taaccgcgag aagatcgaga agattctgac tttcaggatc 3180
ccgtactacg tcggcccact cgctaggggc aactcccgct tcgcttggat gacccgcaag 3240
tcagaggaga cgatcacgcc gtggaacttc gaggaggtgg tcgacaaggg cgctagcgct 3300
cagtcgttca tcgagaggat gacgaatttc gacaagaacc tgccaaatga gaaggtgctc 3360
cctaagcact cgctcctgta cgagtacttc acagtctaca acgagctgac taaggtgaag 3420
tatgtgaccg agggcatgag gaagccggct ttcctgtctg gggagcagaa gaaggccatc 3480
gtggacctcc tgttcaagac caaccggaag gtcacggtta agcagctcaa ggaggactac 3540
ttcaagaaga ttgagtgctt cgattcggtc gagatctctg gcgttgagga ccgcttcaac 3600
gcctccctgg ggacctacca cgatctcctg aagatcatta aggataagga cttcctggac 3660
aacgaggaga atgaggatat cctcgaggac attgtgctga cactcactct gttcgaggac 3720
cgggagatga tcgaggagcg cctgaagact tacgcccatc tcttcgatga caaggtcatg 3780
aagcagctca agaggaggag gtacaccggc tgggggaggc tgagcaggaa gctcatcaac 3840
ggcattcggg acaagcagtc cgggaagacg atcctcgact tcctgaagag cgatggcttc 3900
gcgaaccgca atttcatgca gctgattcac gatgacagcc tcacattcaa ggaggatatc 3960
cagaaggctc aggtgagcgg ccagggggac tcgctgcacg agcatatcgc gaacctcgct 4020
ggctcgccag ctatcaagaa ggggattctg cagaccgtga aggttgtgga cgagctggtg 4080
aaggtcatgg gcaggcacaa gcctgagaac atcgtcattg agatggcccg ggagaatcag 4140
accacgcaga agggccagaa gaactcacgc gagaggatga agaggatcga ggagggcatt 4200
aaggagctgg ggtcccagat cctcaaggag cacccggtgg agaacacgca gctgcagaat 4260
gagaagctct acctgtacta cctccagaat ggccgcgata tgtatgtgga ccaggagctg 4320
gatattaaca ggctcagcga ttacgacgtc gatcatatcg ttccacagtc attcctgaag 4380
gatgactcca ttgacaacaa ggtcctcacc aggtcggaca agaaccgggg caagtctgat 4440
aatgttcctt cagaggaggt cgttaagaag atgaagaact actggcgcca gctcctgaat 4500
gccaagctga tcacgcagcg gaagttcgat aacctcacaa aggctgagag gggcgggctc 4560
tctgagctgg acaaggcggg cttcatcaag aggcagctgg tcgagacacg gcagatcact 4620
aagcacgttg cgcagattct cgactcacgg atgaacacta agtacgatga gaatgacaag 4680
ctgatccgcg aggtgaaggt catcaccctg aagtcaaagc tcgtctccga cttcaggaag 4740
gatttccagt tctacaaggt tcgggagatc aacaattacc accatgccca tgacgcgtac 4800
ctgaacgcgg tggtcggcac agctctgatc aagaagtacc caaagctcga gagcgagttc 4860
gtgtacgggg actacaaggt ttacgatgtg aggaagatga tcgccaagtc ggagcaggag 4920
attggcaagg ctaccgccaa gtacttcttc tactctaaca ttatgaattt cttcaagaca 4980
gagatcactc tggccaatgg cgagatccgg aagcgccccc tcatcgagac gaacggcgag 5040
acgggggaga tcgtgtggga caagggcagg gatttcgcga ccgtcaggaa ggttctctcc 5100
atgccacaag tgaatatcgt caagaagaca gaggtccaga ctggcgggtt ctctaaggag 5160
tcaattctgc ctaagcggaa cagcgacaag ctcatcgccc gcaagaagga ctgggatccg 5220
aagaagtacg gcgggttcga cagccccact gtggcctact cggtcctggt tgtggcgaag 5280
gttgagaagg gcaagtccaa gaagctcaag agcgtgaagg agctgctggg gatcacgatt 5340
atggagcgct ccagcttcga gaagaacccg atcgatttcc tggaggcgaa gggctacaag 5400
gaggtgaaga aggacctgat cattaagctc cccaagtact cactcttcga gctggagaac 5460
ggcaggaagc ggatgctggc ttccgctggc gagctgcaga aggggaacga gctggctctg 5520
ccgtccaagt atgtgaactt cctctacctg gcctcccact acgagaagct caagggcagc 5580
cccgaggaca acgagcagaa gcagctgttc gtcgagcagc acaagcatta cctcgacgag 5640
atcattgagc agatttccga gttctccaag cgcgtgatcc tggccgacgc gaatctggat 5700
aaggtcctct ccgcgtacaa caagcaccgc gacaagccaa tcagggagca ggctgagaat 5760
atcattcatc tcttcaccct gacgaacctc ggcgcccctg ctgctttcaa gtacttcgac 5820
acaactatcg atcgcaagag gtacacaagc actaaggagg tcctggacgc gaccctcatc 5880
caccagtcga ttaccggcct ctacgagacg cgcatcgacc tgtctcagct cgggggcgac 5940
aagcggccag cggcgacgaa gaaggcgggg caggcgaaga agaagaagga attctccggc 6000
ggcagcacca acctgtccga catcatcgag aaggagacgg gcaagcaact cgtgatccag 6060
gagagcatcc tcatgctgcc agaggaggtg gaggaggtca tcggcaacaa gccagagtcc 6120
gacatcctgg tgcacaccgc ctacgacgag tccaccgacg agaacgtcat gctcctgacc 6180
agcgacgccc cagagtacaa gccatgggcc ctcgtcatcc aggacagcaa cggggagaac 6240
aagatcaaga tgctgtcggg ggggagccca aagaagaagc ggaaggtgta g 6291
<210> 178
<211> 2096
<212> PRT
<213> Artificial Sequence
<220>
<223> STEME-2
<400> 178
Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu
1 5 10 15
Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala
20 25 30
Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro
35 40 45
Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg
50 55 60
Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu
65 70 75 80
Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His
85 90 95
Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly
100 105 110
Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His
115 120 125
Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu
130 135 140
Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys
145 150 155 160
Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser
165 170 175
Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser
180 185 190
Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr
195 200 205
Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg
210 215 220
Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly
225 230 235 240
Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala
245 250 255
Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg
260 265 270
Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys
275 280 285
Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val
290 295 300
Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His
305 310 315 320
Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala
325 330 335
Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro Arg Gln
340 345 350
Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly
355 360 365
Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser
370 375 380
Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Glu Ala Ser
385 390 395 400
Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His Ile Phe Thr Ser
405 410 415
Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr Leu Cys Tyr Glu
420 425 430
Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met Asp Gln His Arg
435 440 445
Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys Gly Phe Tyr Gly
450 455 460
Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro Ser Leu Gln Leu
465 470 475 480
Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile Ser Trp Ser Pro
485 490 495
Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala Phe Leu Gln Glu
500 505 510
Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg Ile Tyr Asp Tyr
515 520 525
Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg Asp Ala Gly Ala
530 535 540
Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His Cys Trp Asp Thr
545 550 555 560
Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp Asp Gly Leu Asp
565 570 575
Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala Ile Leu Gln Asn
580 585 590
Gln Gly Asn Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr
595 600 605
Pro Glu Ser Leu Lys Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly
610 615 620
Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro
625 630 635 640
Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys
645 650 655
Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu
660 665 670
Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys
675 680 685
Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys
690 695 700
Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu
705 710 715 720
Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp
725 730 735
Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys
740 745 750
Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu
755 760 765
Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly
770 775 780
Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu
785 790 795 800
Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser
805 810 815
Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg
820 825 830
Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly
835 840 845
Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe
850 855 860
Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys
865 870 875 880
Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp
885 890 895
Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile
900 905 910
Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro
915 920 925
Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu
930 935 940
Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys
945 950 955 960
Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp
965 970 975
Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu
980 985 990
Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
995 1000 1005
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro
1010 1015 1020
His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln
1025 1030 1035
Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu
1040 1045 1050
Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala
1055 1060 1065
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu
1070 1075 1080
Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala
1085 1090 1095
Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn
1100 1105 1110
Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu
1115 1120 1125
Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr
1130 1135 1140
Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys
1145 1150 1155
Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val
1160 1165 1170
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp
1175 1180 1185
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu
1190 1195 1200
Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe
1205 1210 1215
Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu
1220 1225 1230
Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
1235 1240 1245
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu
1250 1255 1260
Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu
1265 1270 1275
Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp
1280 1285 1290
Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu
1295 1300 1305
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala
1310 1315 1320
Gln Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn
1325 1330 1335
Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val
1340 1345 1350
Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro
1355 1360 1365
Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln
1370 1375 1380
Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu
1385 1390 1395
Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val
1400 1405 1410
Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu
1415 1420 1425
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn
1430 1435 1440
Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe
1445 1450 1455
Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp
1460 1465 1470
Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
1475 1480 1485
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu
1490 1495 1500
Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly
1505 1510 1515
Gly Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu
1520 1525 1530
Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp
1535 1540 1545
Ser Arg Met Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg
1550 1555 1560
Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe
1565 1570 1575
Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile Asn Asn Tyr
1580 1585 1590
His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly Thr Ala
1595 1600 1605
Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr Gly
1610 1615 1620
Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys Ser Glu
1625 1630 1635
Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn
1640 1645 1650
Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu
1655 1660 1665
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu
1670 1675 1680
Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val
1685 1690 1695
Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln
1700 1705 1710
Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
1715 1720 1725
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr
1730 1735 1740
Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val
1745 1750 1755
Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys
1760 1765 1770
Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys
1775 1780 1785
Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys
1790 1795 1800
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu
1805 1810 1815
Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala Gly Glu Leu Gln
1820 1825 1830
Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu
1835 1840 1845
Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp
1850 1855 1860
Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His Tyr Leu
1865 1870 1875
Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile
1880 1885 1890
Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys
1895 1900 1905
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His
1910 1915 1920
Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr
1925 1930 1935
Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu
1940 1945 1950
Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
1955 1960 1965
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro
1970 1975 1980
Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys Glu Phe
1985 1990 1995
Ser Gly Gly Ser Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr
2000 2005 2010
Gly Lys Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu
2015 2020 2025
Glu Val Glu Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu
2030 2035 2040
Val His Thr Ala Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu
2045 2050 2055
Leu Thr Ser Asp Ala Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile
2060 2065 2070
Gln Asp Ser Asn Gly Glu Asn Lys Ile Lys Met Leu Ser Gly Gly
2075 2080 2085
Ser Pro Lys Lys Lys Arg Lys Val
2090 2095
<210> 179
<211> 6759
<212> DNA
<213> Artificial Sequence
<220>
<223> STEME-3
<400> 179
atggaggcca gcccggctag cggcccaagg catctcatgg acccgcacat cttcaccagc 60
aacttcaaca acggcatcgg caggcacaag acctacttgt gctacgaggt ggagaggctc 120
gacaacggaa cctccgtgaa gatggaccaa cacagggggt tcctccacaa ccaagccaag 180
aacctcctct gcggcttcta cggcaggcac gccgagttga ggttcctcga cttggtgcca 240
tccctccaac tcgatccagc ccaaatctac cgcgtgacct ggttcatctc ctggtcccca 300
tgcttctcct ggggttgcgc cggcgaggtt cgggctttcc tccaagaaaa cacccacgtc 360
cgcctccgca ttttcgccgc caggatctat gattacgacc ctctctacaa ggaggccctc 420
cagatgctgc gggacgccgg tgctcaggtg agtatcatga cctacgacga gttcaagcac 480
tgctgggaca ccttcgttga ccaccagggc tgcccattcc aaccatggga cggtctggat 540
gaacacagcc aagccttgtc cggcaggctc cgggccatcc tccaaaacca ggggaactcc 600
gggagcgaga cgccaggcac ctccgagtcg gccaccccag aatcttccgg cggatcttct 660
ggaggatcta gcggctccga gacaccagga acatccgaat ccgctacacc agagtcttct 720
ggaggatcta gcggaggatc ttccgaggtg gagttctctc acgagtattg gatgaggcac 780
gctcttacac ttgctaagag agcttgggac gaaagagaag tgccagttgg cgccgttctt 840
gtgcataata atagggtgat cggcgagggt tggaatagac caattggaag gcatgatcca 900
acagctcacg cagagattat ggctctcaga caaggcggcc tcgttatgca gaactacagg 960
ctcattgacg ctacactcta cgtgacactc gaaccttgcg ttatgtgcgc cggagctatg 1020
attcattcta ggattggcag ggtcgtgttt ggagctaggg acgctaaaac aggagccgcc 1080
ggatctctta tggacgtgtt gcatcatcca ggcatgaacc atagggtgga gattacagag 1140
ggcattcttg cagacgagtg cgctgctctt ctttccgatt tcttcaggat gagaaggcag 1200
gagattaagg cccagaagaa ggctcagtct tctacagata gcggaggatc ttccggagga 1260
tctagcggct ccgagacacc aggaacatcc gaaagcgcta caccagaatc tagcggaggc 1320
tcttccggag gatcttctga agtggagttc tcccacgagt attggatgag gcacgctctt 1380
acacttgcta aaagggctag ggacgaaagg gaagttccag ttggagctgt tctcgtgctc 1440
aataacaggg tgattggcga gggttggaat agagccattg gactccatga tccaacagct 1500
cacgcagaga ttatggctct tagacaaggc ggcctcgtta tgcagaatta cagactcatc 1560
gacgccacac tctacgttac cttcgaacct tgcgttatgt gcgccggagc tatgatccat 1620
tctaggattg gcagggtcgt gttcggcgtt agaaacgcta agacaggagc tgcaggctct 1680
cttatggacg ttcttcatta cccaggcatg aatcatagag tggagatcac agaaggcatt 1740
cttgcagacg agtgcgcagc tctcctttgc tatttcttca ggatgccgag gcaagttttc 1800
aacgctcaga agaaggccca gtcttctaca gattccggcg gatcttctgg aggatctagc 1860
ggctccgaga caccaggaac atccgaatcc gctacaccag agtcttctgg aggatctagc 1920
ggaggatctg acaagaagta ctcgatcggc ctcgccattg ggactaactc tgttggctgg 1980
gccgtgatca ccgacgagta caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc 2040
gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc tgttcgacag cggcgagacg 2100
gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc 2160
atctgctacc tgcaggagat tttctccaac gagatggcga aggttgacga ttctttcttc 2220
cacaggctgg aggagtcatt cctcgtggag gaggataaga agcacgagcg gcatccaatc 2280
ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt accctacgat ctaccatctg 2340
cggaagaagc tcgtggactc cacagataag gcggacctcc gcctgatcta cctcgctctg 2400
gcccacatga ttaagttcag gggccatttc ctgatcgagg gggatctcaa cccggacaat 2460
agcgatgttg acaagctgtt catccagctc gtgcagacgt acaaccagct cttcgaggag 2520
aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag 2580
tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg agaagaagaa cggcctgttc 2640
gggaatctca ttgcgctcag cctggggctc acgcccaact tcaagtcgaa tttcgatctc 2700
gctgaggacg ccaagctgca gctctccaag gacacatacg acgatgacct ggataacctc 2760
ctggcccaga tcggcgatca gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac 2820
gccatcctcc tgtctgatat tctcagggtg aacaccgaga ttacgaaggc tccgctctca 2880
gcctccatga tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg 2940
gtcaggcagc agctccccga gaagtacaag gagatcttct tcgatcagtc gaagaacggc 3000
tacgctgggt acattgacgg cggggcctct caggaggagt tctacaagtt catcaagccg 3060
attctggaga agatggacgg cacggaggag ctgctggtga agctcaatcg cgaggacctc 3120
ctgaggaagc agcggacatt cgataacggc agcatcccac accagattca tctcggggag 3180
ctgcacgcta tcctgaggag gcaggaggac ttctaccctt tcctcaagga taaccgcgag 3240
aagatcgaga agattctgac tttcaggatc ccgtactacg tcggcccact cgctaggggc 3300
aactcccgct tcgcttggat gacccgcaag tcagaggaga cgatcacgcc gtggaacttc 3360
gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca tcgagaggat gacgaatttc 3420
gacaagaacc tgccaaatga gaaggtgctc cctaagcact cgctcctgta cgagtacttc 3480
acagtctaca acgagctgac taaggtgaag tatgtgaccg agggcatgag gaagccggct 3540
ttcctgtctg gggagcagaa gaaggccatc gtggacctcc tgttcaagac caaccggaag 3600
gtcacggtta agcagctcaa ggaggactac ttcaagaaga ttgagtgctt cgattcggtc 3660
gagatctctg gcgttgagga ccgcttcaac gcctccctgg ggacctacca cgatctcctg 3720
aagatcatta aggataagga cttcctggac aacgaggaga atgaggatat cctcgaggac 3780
attgtgctga cactcactct gttcgaggac cgggagatga tcgaggagcg cctgaagact 3840
tacgcccatc tcttcgatga caaggtcatg aagcagctca agaggaggag gtacaccggc 3900
tgggggaggc tgagcaggaa gctcatcaac ggcattcggg acaagcagtc cgggaagacg 3960
atcctcgact tcctgaagag cgatggcttc gcgaaccgca atttcatgca gctgattcac 4020
gatgacagcc tcacattcaa ggaggatatc cagaaggctc aggtgagcgg ccagggggac 4080
tcgctgcacg agcatatcgc gaacctcgct ggctcgccag ctatcaagaa ggggattctg 4140
cagaccgtga aggttgtgga cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac 4200
atcgtcattg agatggcccg ggagaatcag accacgcaga agggccagaa gaactcacgc 4260
gagaggatga agaggatcga ggagggcatt aaggagctgg ggtcccagat cctcaaggag 4320
cacccggtgg agaacacgca gctgcagaat gagaagctct acctgtacta cctccagaat 4380
ggccgcgata tgtatgtgga ccaggagctg gatattaaca ggctcagcga ttacgacgtc 4440
gatcatatcg ttccacagtc attcctgaag gatgactcca ttgacaacaa ggtcctcacc 4500
aggtcggaca agaaccgggg caagtctgat aatgttcctt cagaggaggt cgttaagaag 4560
atgaagaact actggcgcca gctcctgaat gccaagctga tcacgcagcg gaagttcgat 4620
aacctcacaa aggctgagag gggcgggctc tctgagctgg acaaggcggg cttcatcaag 4680
aggcagctgg tcgagacacg gcagatcact aagcacgttg cgcagattct cgactcacgg 4740
atgaacacta agtacgatga gaatgacaag ctgatccgcg aggtgaaggt catcaccctg 4800
aagtcaaagc tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgggagatc 4860
aacaattacc accatgccca tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc 4920
aagaagtacc caaagctcga gagcgagttc gtgtacgggg actacaaggt ttacgatgtg 4980
aggaagatga tcgccaagtc ggagcaggag attggcaagg ctaccgccaa gtacttcttc 5040
tactctaaca ttatgaattt cttcaagaca gagatcactc tggccaatgg cgagatccgg 5100
aagcgccccc tcatcgagac gaacggcgag acgggggaga tcgtgtggga caagggcagg 5160
gatttcgcga ccgtcaggaa ggttctctcc atgccacaag tgaatatcgt caagaagaca 5220
gaggtccaga ctggcgggtt ctctaaggag tcaattctgc ctaagcggaa cagcgacaag 5280
ctcatcgccc gcaagaagga ctgggatccg aagaagtacg gcgggttcga cagccccact 5340
gtggcctact cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag 5400
agcgtgaagg agctgctggg gatcacgatt atggagcgct ccagcttcga gaagaacccg 5460
atcgatttcc tggaggcgaa gggctacaag gaggtgaaga aggacctgat cattaagctc 5520
cccaagtact cactcttcga gctggagaac ggcaggaagc ggatgctggc ttccgctggc 5580
gagctgcaga aggggaacga gctggctctg ccgtccaagt atgtgaactt cctctacctg 5640
gcctcccact acgagaagct caagggcagc cccgaggaca acgagcagaa gcagctgttc 5700
gtcgagcagc acaagcatta cctcgacgag atcattgagc agatttccga gttctccaag 5760
cgcgtgatcc tggccgacgc gaatctggat aaggtcctct ccgcgtacaa caagcaccgc 5820
gacaagccaa tcagggagca ggctgagaat atcattcatc tcttcaccct gacgaacctc 5880
ggcgcccctg ctgctttcaa gtacttcgac acaactatcg atcgcaagag gtacacaagc 5940
actaaggagg tcctggacgc gaccctcatc caccagtcga ttaccggcct ctacgagacg 6000
cgcatcgacc tgtctcagct cgggggcgac aagcggccag cggcgacgaa gaaggcgggg 6060
caggcgaaga agaagaagga attcgagggc agaggaagtc ttctaacatg cggtgacgtg 6120
gaggagaatc ccggccctac caacctgtcc gacatcatcg agaaggagac gggcaagcaa 6180
ctcgtgatcc aggagagcat cctcatgctg ccagaggagg tggaggaggt catcggcaac 6240
aagccagagt ccgacatcct ggtgcacacc gcctacgacg agtccaccga cgagaacgtc 6300
atgctcctga ccagcgacgc cccagagtac aagccatggg ccctcgtcat ccaggacagc 6360
aacggggaga acaagatcaa gatgctgtcg ggggggagcc caaagaagaa gcggaaggtg 6420
gagggcagag gaagtcttct aacatgcggt gacgtggagg agaatcccgg ccctaccaac 6480
ctgtccgaca tcatcgagaa ggagacgggc aagcaactcg tgatccagga gagcatcctc 6540
atgctgccag aggaggtgga ggaggtcatc ggcaacaagc cagagtccga catcctggtg 6600
cacaccgcct acgacgagtc caccgacgag aacgtcatgc tcctgaccag cgacgcccca 6660
gagtacaagc catgggccct cgtcatccag gacagcaacg gggagaacaa gatcaagatg 6720
ctgtcggggg ggagcccaaa gaagaagcgg aaggtgtag 6759
<210> 180
<211> 2252
<212> PRT
<213> Artificial Sequence
<220>
<223> STEME-3
<400> 180
Met Glu Ala Ser Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His
1 5 10 15
Ile Phe Thr Ser Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr
20 25 30
Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met
35 40 45
Asp Gln His Arg Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys
50 55 60
Gly Phe Tyr Gly Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro
65 70 75 80
Ser Leu Gln Leu Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile
85 90 95
Ser Trp Ser Pro Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala
100 105 110
Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg
115 120 125
Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg
130 135 140
Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His
145 150 155 160
Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp
165 170 175
Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala
180 185 190
Ile Leu Gln Asn Gln Gly Asn Ser Gly Ser Glu Thr Pro Gly Thr Ser
195 200 205
Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser
210 215 220
Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser
225 230 235 240
Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr
245 250 255
Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg
260 265 270
Glu Val Pro Val Gly Ala Val Leu Val His Asn Asn Arg Val Ile Gly
275 280 285
Glu Gly Trp Asn Arg Pro Ile Gly Arg His Asp Pro Thr Ala His Ala
290 295 300
Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg
305 310 315 320
Leu Ile Asp Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys Val Met Cys
325 330 335
Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Ala
340 345 350
Arg Asp Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His
355 360 365
His Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala
370 375 380
Asp Glu Cys Ala Ala Leu Leu Ser Asp Phe Phe Arg Met Arg Arg Gln
385 390 395 400
Glu Ile Lys Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly
405 410 415
Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser
420 425 430
Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Glu Val
435 440 445
Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys
450 455 460
Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu
465 470 475 480
Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His
485 490 495
Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu
500 505 510
Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe
515 520 525
Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly
530 535 540
Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser
545 550 555 560
Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile
565 570 575
Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe
580 585 590
Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser
595 600 605
Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr
610 615 620
Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser
625 630 635 640
Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn
645 650 655
Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys
660 665 670
Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn
675 680 685
Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr
690 695 700
Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg
705 710 715 720
Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp
725 730 735
Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp
740 745 750
Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val
755 760 765
Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu
770 775 780
Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu
785 790 795 800
Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu
805 810 815
Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln
820 825 830
Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val
835 840 845
Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu
850 855 860
Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe
865 870 875 880
Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser
885 890 895
Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr
900 905 910
Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr
915 920 925
Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu
930 935 940
Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser
945 950 955 960
Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu
965 970 975
Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile
980 985 990
Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly
995 1000 1005
Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu
1010 1015 1020
Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
1025 1030 1035
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro
1040 1045 1050
His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln
1055 1060 1065
Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu
1070 1075 1080
Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala
1085 1090 1095
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu
1100 1105 1110
Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala
1115 1120 1125
Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn
1130 1135 1140
Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu
1145 1150 1155
Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr
1160 1165 1170
Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys
1175 1180 1185
Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val
1190 1195 1200
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp
1205 1210 1215
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu
1220 1225 1230
Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe
1235 1240 1245
Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu
1250 1255 1260
Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
1265 1270 1275
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu
1280 1285 1290
Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu
1295 1300 1305
Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp
1310 1315 1320
Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu
1325 1330 1335
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala
1340 1345 1350
Gln Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn
1355 1360 1365
Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val
1370 1375 1380
Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro
1385 1390 1395
Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln
1400 1405 1410
Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu
1415 1420 1425
Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val
1430 1435 1440
Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu
1445 1450 1455
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn
1460 1465 1470
Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe
1475 1480 1485
Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp
1490 1495 1500
Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
1505 1510 1515
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu
1520 1525 1530
Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly
1535 1540 1545
Gly Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu
1550 1555 1560
Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp
1565 1570 1575
Ser Arg Met Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg
1580 1585 1590
Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe
1595 1600 1605
Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile Asn Asn Tyr
1610 1615 1620
His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly Thr Ala
1625 1630 1635
Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr Gly
1640 1645 1650
Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys Ser Glu
1655 1660 1665
Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn
1670 1675 1680
Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu
1685 1690 1695
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu
1700 1705 1710
Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val
1715 1720 1725
Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln
1730 1735 1740
Thr Gly Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser
1745 1750 1755
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr
1760 1765 1770
Gly Gly Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val
1775 1780 1785
Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys
1790 1795 1800
Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys
1805 1810 1815
Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys
1820 1825 1830
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu
1835 1840 1845
Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala Gly Glu Leu Gln
1850 1855 1860
Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu
1865 1870 1875
Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp
1880 1885 1890
Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His Tyr Leu
1895 1900 1905
Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile
1910 1915 1920
Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys
1925 1930 1935
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His
1940 1945 1950
Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr
1955 1960 1965
Phe Asp Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu
1970 1975 1980
Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
1985 1990 1995
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro
2000 2005 2010
Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys Glu Phe
2015 2020 2025
Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn
2030 2035 2040
Pro Gly Pro Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly
2045 2050 2055
Lys Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu
2060 2065 2070
Val Glu Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val
2075 2080 2085
His Thr Ala Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu Leu
2090 2095 2100
Thr Ser Asp Ala Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile Gln
2105 2110 2115
Asp Ser Asn Gly Glu Asn Lys Ile Lys Met Leu Ser Gly Gly Ser
2120 2125 2130
Pro Lys Lys Lys Arg Lys Val Glu Gly Arg Gly Ser Leu Leu Thr
2135 2140 2145
Cys Gly Asp Val Glu Glu Asn Pro Gly Pro Thr Asn Leu Ser Asp
2150 2155 2160
Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu Val Ile Gln Glu Ser
2165 2170 2175
Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val Ile Gly Asn Lys
2180 2185 2190
Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp Glu Ser Thr
2195 2200 2205
Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu Tyr Lys
2210 2215 2220
Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys Ile
2225 2230 2235
Lys Met Leu Ser Gly Gly Ser Pro Lys Lys Lys Arg Lys Val
2240 2245 2250
<210> 181
<211> 6663
<212> DNA
<213> Artificial Sequence
<220>
<223> STEME-4
<400> 181
atgtccgagg tggagttctc tcacgagtat tggatgaggc acgctcttac acttgctaag 60
agagcttggg acgaaagaga agtgccagtt ggcgccgttc ttgtgcataa taatagggtg 120
atcggcgagg gttggaatag accaattgga aggcatgatc caacagctca cgcagagatt 180
atggctctca gacaaggcgg cctcgttatg cagaactaca ggctcattga cgctacactc 240
tacgtgacac tcgaaccttg cgttatgtgc gccggagcta tgattcattc taggattggc 300
agggtcgtgt ttggagctag ggacgctaaa acaggagccg ccggatctct tatggacgtg 360
ttgcatcatc caggcatgaa ccatagggtg gagattacag agggcattct tgcagacgag 420
tgcgctgctc ttctttccga tttcttcagg atgagaaggc aggagattaa ggcccagaag 480
aaggctcagt cttctacaga tagcggagga tcttccggag gatctagcgg ctccgagaca 540
ccaggaacat ccgaaagcgc tacaccagaa tctagcggag gctcttccgg aggatcttct 600
gaagtggagt tctcccacga gtattggatg aggcacgctc ttacacttgc taaaagggct 660
agggacgaaa gggaagttcc agttggagct gttctcgtgc tcaataacag ggtgattggc 720
gagggttgga atagagccat tggactccat gatccaacag ctcacgcaga gattatggct 780
cttagacaag gcggcctcgt tatgcagaat tacagactca tcgacgccac actctacgtt 840
accttcgaac cttgcgttat gtgcgccgga gctatgatcc attctaggat tggcagggtc 900
gtgttcggcg ttagaaacgc taagacagga gctgcaggct ctcttatgga cgttcttcat 960
tacccaggca tgaatcatag agtggagatc acagaaggca ttcttgcaga cgagtgcgca 1020
gctctccttt gctatttctt caggatgccg aggcaagttt tcaacgctca gaagaaggcc 1080
cagtcttcta cagattccgg cggatcttct ggaggatcta gcggctccga gacaccagga 1140
acatccgaat ccgctacacc agagtcttct ggaggatcta gcggaggatc tgaggccagc 1200
ccggctagcg gcccaaggca tctcatggac ccgcacatct tcaccagcaa cttcaacaac 1260
ggcatcggca ggcacaagac ctacttgtgc tacgaggtgg agaggctcga caacggaacc 1320
tccgtgaaga tggaccaaca cagggggttc ctccacaacc aagccaagaa cctcctctgc 1380
ggcttctacg gcaggcacgc cgagttgagg ttcctcgact tggtgccatc cctccaactc 1440
gatccagccc aaatctaccg cgtgacctgg ttcatctcct ggtccccatg cttctcctgg 1500
ggttgcgccg gcgaggttcg ggctttcctc caagaaaaca cccacgtccg cctccgcatt 1560
ttcgccgcca ggatctatga ttacgaccct ctctacaagg aggccctcca gatgctgcgg 1620
gacgccggtg ctcaggtgag tatcatgacc tacgacgagt tcaagcactg ctgggacacc 1680
ttcgttgacc accagggctg cccattccaa ccatgggacg gtctggatga acacagccaa 1740
gccttgtccg gcaggctccg ggccatcctc caaaaccagg ggaactccgg gagcgagacg 1800
ccaggcacct ccgagtcggc caccccagaa tctgacaaga agtactcgat cggcctcgcc 1860
attgggacta actctgttgg ctgggccgtg atcaccgacg agtacaaggt gccctcaaag 1920
aagttcaagg tcctgggcaa caccgatcgg cattccatca agaagaatct cattggcgct 1980
ctcctgttcg acagcggcga gacggctgag gctacgcggc tcaagcgcac cgcccgcagg 2040
cggtacacgc gcaggaagaa tcgcatctgc tacctgcagg agattttctc caacgagatg 2100
gcgaaggttg acgattcttt cttccacagg ctggaggagt cattcctcgt ggaggaggat 2160
aagaagcacg agcggcatcc aatcttcggc aacattgtcg acgaggttgc ctaccacgag 2220
aagtacccta cgatctacca tctgcggaag aagctcgtgg actccacaga taaggcggac 2280
ctccgcctga tctacctcgc tctggcccac atgattaagt tcaggggcca tttcctgatc 2340
gagggggatc tcaacccgga caatagcgat gttgacaagc tgttcatcca gctcgtgcag 2400
acgtacaacc agctcttcga ggagaacccc attaatgcgt caggcgtcga cgcgaaggct 2460
atcctgtccg ctaggctctc gaagtctcgg cgcctcgaga acctgatcgc ccagctgccg 2520
ggcgagaaga agaacggcct gttcgggaat ctcattgcgc tcagcctggg gctcacgccc 2580
aacttcaagt cgaatttcga tctcgctgag gacgccaagc tgcagctctc caaggacaca 2640
tacgacgatg acctggataa cctcctggcc cagatcggcg atcagtacgc ggacctgttc 2700
ctcgctgcca agaatctgtc ggacgccatc ctcctgtctg atattctcag ggtgaacacc 2760
gagattacga aggctccgct ctcagcctcc atgatcaagc gctacgacga gcaccatcag 2820
gatctgaccc tcctgaaggc gctggtcagg cagcagctcc ccgagaagta caaggagatc 2880
ttcttcgatc agtcgaagaa cggctacgct gggtacattg acggcggggc ctctcaggag 2940
gagttctaca agttcatcaa gccgattctg gagaagatgg acggcacgga ggagctgctg 3000
gtgaagctca atcgcgagga cctcctgagg aagcagcgga cattcgataa cggcagcatc 3060
ccacaccaga ttcatctcgg ggagctgcac gctatcctga ggaggcagga ggacttctac 3120
cctttcctca aggataaccg cgagaagatc gagaagattc tgactttcag gatcccgtac 3180
tacgtcggcc cactcgctag gggcaactcc cgcttcgctt ggatgacccg caagtcagag 3240
gagacgatca cgccgtggaa cttcgaggag gtggtcgaca agggcgctag cgctcagtcg 3300
ttcatcgaga ggatgacgaa tttcgacaag aacctgccaa atgagaaggt gctccctaag 3360
cactcgctcc tgtacgagta cttcacagtc tacaacgagc tgactaaggt gaagtatgtg 3420
accgagggca tgaggaagcc ggctttcctg tctggggagc agaagaaggc catcgtggac 3480
ctcctgttca agaccaaccg gaaggtcacg gttaagcagc tcaaggagga ctacttcaag 3540
aagattgagt gcttcgattc ggtcgagatc tctggcgttg aggaccgctt caacgcctcc 3600
ctggggacct accacgatct cctgaagatc attaaggata aggacttcct ggacaacgag 3660
gagaatgagg atatcctcga ggacattgtg ctgacactca ctctgttcga ggaccgggag 3720
atgatcgagg agcgcctgaa gacttacgcc catctcttcg atgacaaggt catgaagcag 3780
ctcaagagga ggaggtacac cggctggggg aggctgagca ggaagctcat caacggcatt 3840
cgggacaagc agtccgggaa gacgatcctc gacttcctga agagcgatgg cttcgcgaac 3900
cgcaatttca tgcagctgat tcacgatgac agcctcacat tcaaggagga tatccagaag 3960
gctcaggtga gcggccaggg ggactcgctg cacgagcata tcgcgaacct cgctggctcg 4020
ccagctatca agaaggggat tctgcagacc gtgaaggttg tggacgagct ggtgaaggtc 4080
atgggcaggc acaagcctga gaacatcgtc attgagatgg cccgggagaa tcagaccacg 4140
cagaagggcc agaagaactc acgcgagagg atgaagagga tcgaggaggg cattaaggag 4200
ctggggtccc agatcctcaa ggagcacccg gtggagaaca cgcagctgca gaatgagaag 4260
ctctacctgt actacctcca gaatggccgc gatatgtatg tggaccagga gctggatatt 4320
aacaggctca gcgattacga cgtcgatcat atcgttccac agtcattcct gaaggatgac 4380
tccattgaca acaaggtcct caccaggtcg gacaagaacc ggggcaagtc tgataatgtt 4440
ccttcagagg aggtcgttaa gaagatgaag aactactggc gccagctcct gaatgccaag 4500
ctgatcacgc agcggaagtt cgataacctc acaaaggctg agaggggcgg gctctctgag 4560
ctggacaagg cgggcttcat caagaggcag ctggtcgaga cacggcagat cactaagcac 4620
gttgcgcaga ttctcgactc acggatgaac actaagtacg atgagaatga caagctgatc 4680
cgcgaggtga aggtcatcac cctgaagtca aagctcgtct ccgacttcag gaaggatttc 4740
cagttctaca aggttcggga gatcaacaat taccaccatg cccatgacgc gtacctgaac 4800
gcggtggtcg gcacagctct gatcaagaag tacccaaagc tcgagagcga gttcgtgtac 4860
ggggactaca aggtttacga tgtgaggaag atgatcgcca agtcggagca ggagattggc 4920
aaggctaccg ccaagtactt cttctactct aacattatga atttcttcaa gacagagatc 4980
actctggcca atggcgagat ccggaagcgc cccctcatcg agacgaacgg cgagacgggg 5040
gagatcgtgt gggacaaggg cagggatttc gcgaccgtca ggaaggttct ctccatgcca 5100
caagtgaata tcgtcaagaa gacagaggtc cagactggcg ggttctctaa ggagtcaatt 5160
ctgcctaagc ggaacagcga caagctcatc gcccgcaaga aggactggga tccgaagaag 5220
tacggcgggt tcgacagccc cactgtggcc tactcggtcc tggttgtggc gaaggttgag 5280
aagggcaagt ccaagaagct caagagcgtg aaggagctgc tggggatcac gattatggag 5340
cgctccagct tcgagaagaa cccgatcgat ttcctggagg cgaagggcta caaggaggtg 5400
aagaaggacc tgatcattaa gctccccaag tactcactct tcgagctgga gaacggcagg 5460
aagcggatgc tggcttccgc tggcgagctg cagaagggga acgagctggc tctgccgtcc 5520
aagtatgtga acttcctcta cctggcctcc cactacgaga agctcaaggg cagccccgag 5580
gacaacgagc agaagcagct gttcgtcgag cagcacaagc attacctcga cgagatcatt 5640
gagcagattt ccgagttctc caagcgcgtg atcctggccg acgcgaatct ggataaggtc 5700
ctctccgcgt acaacaagca ccgcgacaag ccaatcaggg agcaggctga gaatatcatt 5760
catctcttca ccctgacgaa cctcggcgcc cctgctgctt tcaagtactt cgacacaact 5820
atcgatcgca agaggtacac aagcactaag gaggtcctgg acgcgaccct catccaccag 5880
tcgattaccg gcctctacga gacgcgcatc gacctgtctc agctcggggg cgacaagcgg 5940
ccagcggcga cgaagaaggc ggggcaggcg aagaagaaga aggaattcga gggcagagga 6000
agtcttctaa catgcggtga cgtggaggag aatcccggcc ctaccaacct gtccgacatc 6060
atcgagaagg agacgggcaa gcaactcgtg atccaggaga gcatcctcat gctgccagag 6120
gaggtggagg aggtcatcgg caacaagcca gagtccgaca tcctggtgca caccgcctac 6180
gacgagtcca ccgacgagaa cgtcatgctc ctgaccagcg acgccccaga gtacaagcca 6240
tgggccctcg tcatccagga cagcaacggg gagaacaaga tcaagatgct gtcggggggg 6300
agcccaaaga agaagcggaa ggtggagggc agaggaagtc ttctaacatg cggtgacgtg 6360
gaggagaatc ccggccctac caacctgtcc gacatcatcg agaaggagac gggcaagcaa 6420
ctcgtgatcc aggagagcat cctcatgctg ccagaggagg tggaggaggt catcggcaac 6480
aagccagagt ccgacatcct ggtgcacacc gcctacgacg agtccaccga cgagaacgtc 6540
atgctcctga ccagcgacgc cccagagtac aagccatggg ccctcgtcat ccaggacagc 6600
aacggggaga acaagatcaa gatgctgtcg ggggggagcc caaagaagaa gcggaaggtg 6660
tag 6663
<210> 182
<211> 2220
<212> PRT
<213> Artificial Sequence
<220>
<223> STEME-4
<400> 182
Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu
1 5 10 15
Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala
20 25 30
Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro
35 40 45
Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg
50 55 60
Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu
65 70 75 80
Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His
85 90 95
Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly
100 105 110
Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His
115 120 125
Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu
130 135 140
Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys
145 150 155 160
Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser
165 170 175
Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser
180 185 190
Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr
195 200 205
Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg
210 215 220
Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly
225 230 235 240
Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala
245 250 255
Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg
260 265 270
Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys
275 280 285
Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val
290 295 300
Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His
305 310 315 320
Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala
325 330 335
Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro Arg Gln
340 345 350
Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly
355 360 365
Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser
370 375 380
Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Glu Ala Ser
385 390 395 400
Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His Ile Phe Thr Ser
405 410 415
Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr Leu Cys Tyr Glu
420 425 430
Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met Asp Gln His Arg
435 440 445
Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys Gly Phe Tyr Gly
450 455 460
Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro Ser Leu Gln Leu
465 470 475 480
Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile Ser Trp Ser Pro
485 490 495
Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala Phe Leu Gln Glu
500 505 510
Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg Ile Tyr Asp Tyr
515 520 525
Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg Asp Ala Gly Ala
530 535 540
Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His Cys Trp Asp Thr
545 550 555 560
Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp Asp Gly Leu Asp
565 570 575
Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala Ile Leu Gln Asn
580 585 590
Gln Gly Asn Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr
595 600 605
Pro Glu Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn
610 615 620
Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys
625 630 635 640
Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn
645 650 655
Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr
660 665 670
Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg
675 680 685
Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp
690 695 700
Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp
705 710 715 720
Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val
725 730 735
Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu
740 745 750
Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu
755 760 765
Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu
770 775 780
Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln
785 790 795 800
Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val
805 810 815
Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu
820 825 830
Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe
835 840 845
Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser
850 855 860
Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr
865 870 875 880
Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr
885 890 895
Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu
900 905 910
Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser
915 920 925
Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu
930 935 940
Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile
945 950 955 960
Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly
965 970 975
Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys
980 985 990
Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu
995 1000 1005
Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln
1010 1015 1020
Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp
1025 1030 1035
Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile
1040 1045 1050
Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly
1055 1060 1065
Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr Ile
1070 1075 1080
Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala
1085 1090 1095
Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro
1100 1105 1110
Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe
1115 1120 1125
Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly
1130 1135 1140
Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile
1145 1150 1155
Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln
1160 1165 1170
Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val
1175 1180 1185
Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr
1190 1195 1200
Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp
1205 1210 1215
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu
1220 1225 1230
Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr
1235 1240 1245
Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg
1250 1255 1260
Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn
1265 1270 1275
Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu
1280 1285 1290
Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His
1295 1300 1305
Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val
1310 1315 1320
Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala
1325 1330 1335
Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val
1340 1345 1350
Val Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn
1355 1360 1365
Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly
1370 1375 1380
Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile
1385 1390 1395
Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn
1400 1405 1410
Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn
1415 1420 1425
Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu
1430 1435 1440
Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys
1445 1450 1455
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn
1460 1465 1470
Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys
1475 1480 1485
Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr
1490 1495 1500
Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu
1505 1510 1515
Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu
1520 1525 1530
Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg
1535 1540 1545
Met Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val
1550 1555 1560
Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys
1565 1570 1575
Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His
1580 1585 1590
Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly Thr Ala Leu Ile
1595 1600 1605
Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr Gly Asp Tyr
1610 1615 1620
Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys Ser Glu Gln Glu
1625 1630 1635
Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn Ile Met
1640 1645 1650
Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu Ile Arg
1655 1660 1665
Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile Val
1670 1675 1680
Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser
1685 1690 1695
Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly
1700 1705 1710
Gly Phe Ser Lys Glu Ser Ile Leu Pro Lys Arg Asn Ser Asp Lys
1715 1720 1725
Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly
1730 1735 1740
Phe Asp Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys
1745 1750 1755
Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu
1760 1765 1770
Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro
1775 1780 1785
Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp
1790 1795 1800
Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn
1805 1810 1815
Gly Arg Lys Arg Met Leu Ala Ser Ala Gly Glu Leu Gln Lys Gly
1820 1825 1830
Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu
1835 1840 1845
Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp Asn Glu
1850 1855 1860
Gln Lys Gln Leu Phe Val Glu Gln His Lys His Tyr Leu Asp Glu
1865 1870 1875
Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile Leu Ala
1880 1885 1890
Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys His Arg
1895 1900 1905
Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His Leu Phe
1910 1915 1920
Thr Leu Thr Asn Leu Gly Ala Pro Ala Ala Phe Lys Tyr Phe Asp
1925 1930 1935
Thr Thr Ile Asp Arg Lys Arg Tyr Thr Ser Thr Lys Glu Val Leu
1940 1945 1950
Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr
1955 1960 1965
Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro Ala Ala
1970 1975 1980
Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys Glu Phe Glu Gly
1985 1990 1995
Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn Pro Gly
2000 2005 2010
Pro Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln
2015 2020 2025
Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu
2030 2035 2040
Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr
2045 2050 2055
Ala Tyr Asp Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser
2060 2065 2070
Asp Ala Pro Glu Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser
2075 2080 2085
Asn Gly Glu Asn Lys Ile Lys Met Leu Ser Gly Gly Ser Pro Lys
2090 2095 2100
Lys Lys Arg Lys Val Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly
2105 2110 2115
Asp Val Glu Glu Asn Pro Gly Pro Thr Asn Leu Ser Asp Ile Ile
2120 2125 2130
Glu Lys Glu Thr Gly Lys Gln Leu Val Ile Gln Glu Ser Ile Leu
2135 2140 2145
Met Leu Pro Glu Glu Val Glu Glu Val Ile Gly Asn Lys Pro Glu
2150 2155 2160
Ser Asp Ile Leu Val His Thr Ala Tyr Asp Glu Ser Thr Asp Glu
2165 2170 2175
Asn Val Met Leu Leu Thr Ser Asp Ala Pro Glu Tyr Lys Pro Trp
2180 2185 2190
Ala Leu Val Ile Gln Asp Ser Asn Gly Glu Asn Lys Ile Lys Met
2195 2200 2205
Leu Ser Gly Gly Ser Pro Lys Lys Lys Arg Lys Val
2210 2215 2220
<210> 183
<211> 6384
<212> DNA
<213> Artificial Sequence
<220>
<223> STEME-NG
<400> 183
atggaggcca gcccggctag cggcccaagg catctcatgg acccgcacat cttcaccagc 60
aacttcaaca acggcatcgg caggcacaag acctacttgt gctacgaggt ggagaggctc 120
gacaacggaa cctccgtgaa gatggaccaa cacagggggt tcctccacaa ccaagccaag 180
aacctcctct gcggcttcta cggcaggcac gccgagttga ggttcctcga cttggtgcca 240
tccctccaac tcgatccagc ccaaatctac cgcgtgacct ggttcatctc ctggtcccca 300
tgcttctcct ggggttgcgc cggcgaggtt cgggctttcc tccaagaaaa cacccacgtc 360
cgcctccgca ttttcgccgc caggatctat gattacgacc ctctctacaa ggaggccctc 420
cagatgctgc gggacgccgg tgctcaggtg agtatcatga cctacgacga gttcaagcac 480
tgctgggaca ccttcgttga ccaccagggc tgcccattcc aaccatggga cggtctggat 540
gaacacagcc aagccttgtc cggcaggctc cgggccatcc tccaaaacca ggggaactcc 600
gggagcgaga cgccaggcac ctccgagtcg gccaccccag aatcttccgg cggatcttct 660
ggaggatcta gcggctccga gacaccagga acatccgaat ccgctacacc agagtcttct 720
ggaggatcta gcggaggatc ttccgaggtg gagttctctc acgagtattg gatgaggcac 780
gctcttacac ttgctaagag agcttgggac gaaagagaag tgccagttgg cgccgttctt 840
gtgcataata atagggtgat cggcgagggt tggaatagac caattggaag gcatgatcca 900
acagctcacg cagagattat ggctctcaga caaggcggcc tcgttatgca gaactacagg 960
ctcattgacg ctacactcta cgtgacactc gaaccttgcg ttatgtgcgc cggagctatg 1020
attcattcta ggattggcag ggtcgtgttt ggagctaggg acgctaaaac aggagccgcc 1080
ggatctctta tggacgtgtt gcatcatcca ggcatgaacc atagggtgga gattacagag 1140
ggcattcttg cagacgagtg cgctgctctt ctttccgatt tcttcaggat gagaaggcag 1200
gagattaagg cccagaagaa ggctcagtct tctacagata gcggaggatc ttccggagga 1260
tctagcggct ccgagacacc aggaacatcc gaaagcgcta caccagaatc tagcggaggc 1320
tcttccggag gatcttctga agtggagttc tcccacgagt attggatgag gcacgctctt 1380
acacttgcta aaagggctag ggacgaaagg gaagttccag ttggagctgt tctcgtgctc 1440
aataacaggg tgattggcga gggttggaat agagccattg gactccatga tccaacagct 1500
cacgcagaga ttatggctct tagacaaggc ggcctcgtta tgcagaatta cagactcatc 1560
gacgccacac tctacgttac cttcgaacct tgcgttatgt gcgccggagc tatgatccat 1620
tctaggattg gcagggtcgt gttcggcgtt agaaacgcta agacaggagc tgcaggctct 1680
cttatggacg ttcttcatta cccaggcatg aatcatagag tggagatcac agaaggcatt 1740
cttgcagacg agtgcgcagc tctcctttgc tatttcttca ggatgccgag gcaagttttc 1800
aacgctcaga agaaggccca gtcttctaca gattccggcg gatcttctgg aggatctagc 1860
ggctccgaga caccaggaac atccgaatcc gctacaccag agtcttctgg aggatctagc 1920
ggaggatctg acaagaagta ctcgatcggc ctcgccattg ggactaactc tgttggctgg 1980
gccgtgatca ccgacgagta caaggtgccc tcaaagaagt tcaaggtcct gggcaacacc 2040
gatcggcatt ccatcaagaa gaatctcatt ggcgctctcc tgttcgacag cggcgagacg 2100
gctgaggcta cgcggctcaa gcgcaccgcc cgcaggcggt acacgcgcag gaagaatcgc 2160
atctgctacc tgcaggagat tttctccaac gagatggcga aggttgacga ttctttcttc 2220
cacaggctgg aggagtcatt cctcgtggag gaggataaga agcacgagcg gcatccaatc 2280
ttcggcaaca ttgtcgacga ggttgcctac cacgagaagt accctacgat ctaccatctg 2340
cggaagaagc tcgtggactc cacagataag gcggacctcc gcctgatcta cctcgctctg 2400
gcccacatga ttaagttcag gggccatttc ctgatcgagg gggatctcaa cccggacaat 2460
agcgatgttg acaagctgtt catccagctc gtgcagacgt acaaccagct cttcgaggag 2520
aaccccatta atgcgtcagg cgtcgacgcg aaggctatcc tgtccgctag gctctcgaag 2580
tctcggcgcc tcgagaacct gatcgcccag ctgccgggcg agaagaagaa cggcctgttc 2640
gggaatctca ttgcgctcag cctggggctc acgcccaact tcaagtcgaa tttcgatctc 2700
gctgaggacg ccaagctgca gctctccaag gacacatacg acgatgacct ggataacctc 2760
ctggcccaga tcggcgatca gtacgcggac ctgttcctcg ctgccaagaa tctgtcggac 2820
gccatcctcc tgtctgatat tctcagggtg aacaccgaga ttacgaaggc tccgctctca 2880
gcctccatga tcaagcgcta cgacgagcac catcaggatc tgaccctcct gaaggcgctg 2940
gtcaggcagc agctccccga gaagtacaag gagatcttct tcgatcagtc gaagaacggc 3000
tacgctgggt acattgacgg cggggcctct caggaggagt tctacaagtt catcaagccg 3060
attctggaga agatggacgg cacggaggag ctgctggtga agctcaatcg cgaggacctc 3120
ctgaggaagc agcggacatt cgataacggc agcatcccac accagattca tctcggggag 3180
ctgcacgcta tcctgaggag gcaggaggac ttctaccctt tcctcaagga taaccgcgag 3240
aagatcgaga agattctgac tttcaggatc ccgtactacg tcggcccact cgctaggggc 3300
aactcccgct tcgcttggat gacccgcaag tcagaggaga cgatcacgcc gtggaacttc 3360
gaggaggtgg tcgacaaggg cgctagcgct cagtcgttca tcgagaggat gacgaatttc 3420
gacaagaacc tgccaaatga gaaggtgctc cctaagcact cgctcctgta cgagtacttc 3480
acagtctaca acgagctgac taaggtgaag tatgtgaccg agggcatgag gaagccggct 3540
ttcctgtctg gggagcagaa gaaggccatc gtggacctcc tgttcaagac caaccggaag 3600
gtcacggtta agcagctcaa ggaggactac ttcaagaaga ttgagtgctt cgattcggtc 3660
gagatctctg gcgttgagga ccgcttcaac gcctccctgg ggacctacca cgatctcctg 3720
aagatcatta aggataagga cttcctggac aacgaggaga atgaggatat cctcgaggac 3780
attgtgctga cactcactct gttcgaggac cgggagatga tcgaggagcg cctgaagact 3840
tacgcccatc tcttcgatga caaggtcatg aagcagctca agaggaggag gtacaccggc 3900
tgggggaggc tgagcaggaa gctcatcaac ggcattcggg acaagcagtc cgggaagacg 3960
atcctcgact tcctgaagag cgatggcttc gcgaaccgca atttcatgca gctgattcac 4020
gatgacagcc tcacattcaa ggaggatatc cagaaggctc aggtgagcgg ccagggggac 4080
tcgctgcacg agcatatcgc gaacctcgct ggctcgccag ctatcaagaa ggggattctg 4140
cagaccgtga aggttgtgga cgagctggtg aaggtcatgg gcaggcacaa gcctgagaac 4200
atcgtcattg agatggcccg ggagaatcag accacgcaga agggccagaa gaactcacgc 4260
gagaggatga agaggatcga ggagggcatt aaggagctgg ggtcccagat cctcaaggag 4320
cacccggtgg agaacacgca gctgcagaat gagaagctct acctgtacta cctccagaat 4380
ggccgcgata tgtatgtgga ccaggagctg gatattaaca ggctcagcga ttacgacgtc 4440
gatcatatcg ttccacagtc attcctgaag gatgactcca ttgacaacaa ggtcctcacc 4500
aggtcggaca agaaccgggg caagtctgat aatgttcctt cagaggaggt cgttaagaag 4560
atgaagaact actggcgcca gctcctgaat gccaagctga tcacgcagcg gaagttcgat 4620
aacctcacaa aggctgagag gggcgggctc tctgagctgg acaaggcggg cttcatcaag 4680
aggcagctgg tcgagacacg gcagatcact aagcacgttg cgcagattct cgactcacgg 4740
atgaacacta agtacgatga gaatgacaag ctgatccgcg aggtgaaggt catcaccctg 4800
aagtcaaagc tcgtctccga cttcaggaag gatttccagt tctacaaggt tcgggagatc 4860
aacaattacc accatgccca tgacgcgtac ctgaacgcgg tggtcggcac agctctgatc 4920
aagaagtacc caaagctcga gagcgagttc gtgtacgggg actacaaggt ttacgatgtg 4980
aggaagatga tcgccaagtc ggagcaggag attggcaagg ctaccgccaa gtacttcttc 5040
tactctaaca ttatgaattt cttcaagaca gagatcactc tggccaatgg cgagatccgg 5100
aagcgccccc tcatcgagac gaacggcgag acgggggaga tcgtgtggga caagggcagg 5160
gatttcgcga ccgtcaggaa ggttctctcc atgccacaag tgaatatcgt caagaagaca 5220
gaggtccaga ctggcgggtt ctctaaggag tcaattcggc ctaagcggaa cagcgacaag 5280
ctcatcgccc gcaagaagga ctgggatccg aagaagtacg gcgggttcgt cagccccact 5340
gtggcctact cggtcctggt tgtggcgaag gttgagaagg gcaagtccaa gaagctcaag 5400
agcgtgaagg agctgctggg gatcacgatt atggagcgct ccagcttcga gaagaacccg 5460
atcgatttcc tggaggcgaa gggctacaag gaggtgaaga aggacctgat cattaagctc 5520
cccaagtact cactcttcga gctggagaac ggcaggaagc ggatgctggc ttccgctcgc 5580
ttcctgcaga aggggaacga gctggctctg ccgtccaagt atgtgaactt cctctacctg 5640
gcctcccact acgagaagct caagggcagc cccgaggaca acgagcagaa gcagctgttc 5700
gtcgagcagc acaagcatta cctcgacgag atcattgagc agatttccga gttctccaag 5760
cgcgtgatcc tggccgacgc gaatctggat aaggtcctct ccgcgtacaa caagcaccgc 5820
gacaagccaa tcagggagca ggctgagaat atcattcatc tcttcaccct gacgaacctc 5880
ggcgcccctc gtgctttcaa gtacttcgac acaactatcg atcgcaaggt atacagaagc 5940
actaaggagg tcctggacgc gaccctcatc caccagtcga ttaccggcct ctacgagacg 6000
cgcatcgacc tgtctcagct cgggggcgac aagcggccag cggcgacgaa gaaggcgggg 6060
caggcgaaga agaagaagac ccgcgactcc ggcggcagca cgaacctctc cgacatcatc 6120
gagaaggaga cgggcaagca gctcgtgatc caggagagca tcctcatgct gccggaggag 6180
gtggaggagg tcatcggcaa caagcccgag tccgacatcc tcgtgcacac cgcctacgac 6240
gagtccacgg acgagaacgt catgctcctg acgagcgacg ctccagagta caagccatgg 6300
gctctcgtga tccaggacag caacggcgag aacaagatca agatgctgtc cggcggctcc 6360
ccaaagaaga agcggaaggt gtag 6384
<210> 184
<211> 2127
<212> PRT
<213> Artificial Sequence
<220>
<223> STEME-NG
<400> 184
Met Glu Ala Ser Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His
1 5 10 15
Ile Phe Thr Ser Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr
20 25 30
Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met
35 40 45
Asp Gln His Arg Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys
50 55 60
Gly Phe Tyr Gly Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro
65 70 75 80
Ser Leu Gln Leu Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile
85 90 95
Ser Trp Ser Pro Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala
100 105 110
Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg
115 120 125
Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg
130 135 140
Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His
145 150 155 160
Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp
165 170 175
Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala
180 185 190
Ile Leu Gln Asn Gln Gly Asn Ser Gly Ser Glu Thr Pro Gly Thr Ser
195 200 205
Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser
210 215 220
Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser
225 230 235 240
Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr
245 250 255
Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg
260 265 270
Glu Val Pro Val Gly Ala Val Leu Val His Asn Asn Arg Val Ile Gly
275 280 285
Glu Gly Trp Asn Arg Pro Ile Gly Arg His Asp Pro Thr Ala His Ala
290 295 300
Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg
305 310 315 320
Leu Ile Asp Ala Thr Leu Tyr Val Thr Leu Glu Pro Cys Val Met Cys
325 330 335
Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Ala
340 345 350
Arg Asp Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His
355 360 365
His Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala
370 375 380
Asp Glu Cys Ala Ala Leu Leu Ser Asp Phe Phe Arg Met Arg Arg Gln
385 390 395 400
Glu Ile Lys Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly
405 410 415
Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser
420 425 430
Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Ser Glu Val
435 440 445
Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu Thr Leu Ala Lys
450 455 460
Arg Ala Arg Asp Glu Arg Glu Val Pro Val Gly Ala Val Leu Val Leu
465 470 475 480
Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Ala Ile Gly Leu His
485 490 495
Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg Gln Gly Gly Leu
500 505 510
Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe
515 520 525
Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His Ser Arg Ile Gly
530 535 540
Arg Val Val Phe Gly Val Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser
545 550 555 560
Leu Met Asp Val Leu His Tyr Pro Gly Met Asn His Arg Val Glu Ile
565 570 575
Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe
580 585 590
Phe Arg Met Pro Arg Gln Val Phe Asn Ala Gln Lys Lys Ala Gln Ser
595 600 605
Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr
610 615 620
Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser
625 630 635 640
Gly Gly Ser Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn
645 650 655
Ser Val Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys
660 665 670
Lys Phe Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn
675 680 685
Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr
690 695 700
Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg
705 710 715 720
Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp
725 730 735
Asp Ser Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp
740 745 750
Lys Lys His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val
755 760 765
Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu
770 775 780
Val Asp Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu
785 790 795 800
Ala His Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu
805 810 815
Asn Pro Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln
820 825 830
Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val
835 840 845
Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu
850 855 860
Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe
865 870 875 880
Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser
885 890 895
Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr
900 905 910
Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr
915 920 925
Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu
930 935 940
Ser Asp Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser
945 950 955 960
Ala Ser Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu
965 970 975
Leu Lys Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile
980 985 990
Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly
995 1000 1005
Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu
1010 1015 1020
Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu
1025 1030 1035
Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro
1040 1045 1050
His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg Arg Gln
1055 1060 1065
Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile Glu
1070 1075 1080
Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala
1085 1090 1095
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu
1100 1105 1110
Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala
1115 1120 1125
Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn
1130 1135 1140
Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu
1145 1150 1155
Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr
1160 1165 1170
Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys
1175 1180 1185
Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val
1190 1195 1200
Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp
1205 1210 1215
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu
1220 1225 1230
Gly Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe
1235 1240 1245
Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu
1250 1255 1260
Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu
1265 1270 1275
Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met Lys Gln Leu
1280 1285 1290
Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu
1295 1300 1305
Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu Asp
1310 1315 1320
Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu
1325 1330 1335
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala
1340 1345 1350
Gln Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn
1355 1360 1365
Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val
1370 1375 1380
Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro
1385 1390 1395
Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln
1400 1405 1410
Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu Glu
1415 1420 1425
Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val
1430 1435 1440
Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu
1445 1450 1455
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn
1460 1465 1470
Arg Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe
1475 1480 1485
Leu Lys Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp
1490 1495 1500
Lys Asn Arg Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val
1505 1510 1515
Lys Lys Met Lys Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu
1520 1525 1530
Ile Thr Gln Arg Lys Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly
1535 1540 1545
Gly Leu Ser Glu Leu Asp Lys Ala Gly Phe Ile Lys Arg Gln Leu
1550 1555 1560
Val Glu Thr Arg Gln Ile Thr Lys His Val Ala Gln Ile Leu Asp
1565 1570 1575
Ser Arg Met Asn Thr Lys Tyr Asp Glu Asn Asp Lys Leu Ile Arg
1580 1585 1590
Glu Val Lys Val Ile Thr Leu Lys Ser Lys Leu Val Ser Asp Phe
1595 1600 1605
Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu Ile Asn Asn Tyr
1610 1615 1620
His His Ala His Asp Ala Tyr Leu Asn Ala Val Val Gly Thr Ala
1625 1630 1635
Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu Phe Val Tyr Gly
1640 1645 1650
Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile Ala Lys Ser Glu
1655 1660 1665
Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe Phe Tyr Ser Asn
1670 1675 1680
Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu Ala Asn Gly Glu
1685 1690 1695
Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly Glu Thr Gly Glu
1700 1705 1710
Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr Val Arg Lys Val
1715 1720 1725
Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys Thr Glu Val Gln
1730 1735 1740
Thr Gly Gly Phe Ser Lys Glu Ser Ile Arg Pro Lys Arg Asn Ser
1745 1750 1755
Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp Pro Lys Lys Tyr
1760 1765 1770
Gly Gly Phe Val Ser Pro Thr Val Ala Tyr Ser Val Leu Val Val
1775 1780 1785
Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu Lys Ser Val Lys
1790 1795 1800
Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser Ser Phe Glu Lys
1805 1810 1815
Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr Lys Glu Val Lys
1820 1825 1830
Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser Leu Phe Glu Leu
1835 1840 1845
Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala Arg Phe Leu Gln
1850 1855 1860
Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr Val Asn Phe Leu
1865 1870 1875
Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly Ser Pro Glu Asp
1880 1885 1890
Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His Lys His Tyr Leu
1895 1900 1905
Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser Lys Arg Val Ile
1910 1915 1920
Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser Ala Tyr Asn Lys
1925 1930 1935
His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu Asn Ile Ile His
1940 1945 1950
Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Arg Ala Phe Lys Tyr
1955 1960 1965
Phe Asp Thr Thr Ile Asp Arg Lys Val Tyr Arg Ser Thr Lys Glu
1970 1975 1980
Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile Thr Gly Leu Tyr
1985 1990 1995
Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly Asp Lys Arg Pro
2000 2005 2010
Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys Lys Lys Thr Arg
2015 2020 2025
Asp Ser Gly Gly Ser Thr Asn Leu Ser Asp Ile Ile Glu Lys Glu
2030 2035 2040
Thr Gly Lys Gln Leu Val Ile Gln Glu Ser Ile Leu Met Leu Pro
2045 2050 2055
Glu Glu Val Glu Glu Val Ile Gly Asn Lys Pro Glu Ser Asp Ile
2060 2065 2070
Leu Val His Thr Ala Tyr Asp Glu Ser Thr Asp Glu Asn Val Met
2075 2080 2085
Leu Leu Thr Ser Asp Ala Pro Glu Tyr Lys Pro Trp Ala Leu Val
2090 2095 2100
Ile Gln Asp Ser Asn Gly Glu Asn Lys Ile Lys Met Leu Ser Gly
2105 2110 2115
Gly Ser Pro Lys Lys Lys Arg Lys Val
2120 2125
<210> 185
<211> 5103
<212> DNA
<213> Artificial Sequence
<220>
<223> A3A-PBE-NG
<400> 185
atggaggcca gcccggctag cggcccaagg catctcatgg acccgcacat cttcaccagc 60
aacttcaaca acggcatcgg caggcacaag acctacttgt gctacgaggt ggagaggctc 120
gacaacggaa cctccgtgaa gatggaccaa cacagggggt tcctccacaa ccaagccaag 180
aacctcctct gcggcttcta cggcaggcac gccgagttga ggttcctcga cttggtgcca 240
tccctccaac tcgatccagc ccaaatctac cgcgtgacct ggttcatctc ctggtcccca 300
tgcttctcct ggggttgcgc cggcgaggtt cgggctttcc tccaagaaaa cacccacgtc 360
cgcctccgca ttttcgccgc caggatctat gattacgacc ctctctacaa ggaggccctc 420
cagatgctgc gggacgccgg tgctcaggtg agtatcatga cctacgacga gttcaagcac 480
tgctgggaca ccttcgttga ccaccagggc tgcccattcc aaccatggga cggtctggat 540
gaacacagcc aagccttgtc cggcaggctc cgggccatcc tccaaaacca ggggaactcc 600
gggagcgaga cgccaggcac ctccgagtcg gccaccccag aatctcttaa ggacaagaag 660
tactcgatcg gcctcgccat tgggactaac tctgttggct gggccgtgat caccgacgag 720
tacaaggtgc cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag 780
aagaatctca ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc 840
aagcgcaccg cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag 900
attttctcca acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca 960
ttcctcgtgg aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac 1020
gaggttgcct accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac 1080
tccacagata aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc 1140
aggggccatt tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg 1200
ttcatccagc tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca 1260
ggcgtcgacg cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac 1320
ctgatcgccc agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc 1380
agcctggggc tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg 1440
cagctctcca aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat 1500
cagtacgcgg acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat 1560
attctcaggg tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc 1620
tacgacgagc accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc 1680
gagaagtaca aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac 1740
ggcggggcct ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac 1800
ggcacggagg agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca 1860
ttcgataacg gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg 1920
aggcaggagg acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg 1980
actttcagga tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg 2040
atgacccgca agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag 2100
ggcgctagcg ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat 2160
gagaaggtgc tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg 2220
actaaggtga agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag 2280
aagaaggcca tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc 2340
aaggaggact acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag 2400
gaccgcttca acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag 2460
gacttcctgg acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact 2520
ctgttcgagg accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat 2580
gacaaggtca tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg 2640
aagctcatca acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag 2700
agcgatggct tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc 2760
aaggaggata tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc 2820
gcgaacctcg ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg 2880
gacgagctgg tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc 2940
cgggagaatc agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc 3000
gaggagggca ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg 3060
cagctgcaga atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg 3120
gaccaggagc tggatattaa caggctcagc gattacgacg tcgatcatat cgttccacag 3180
tcattcctga aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg 3240
ggcaagtctg ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc 3300
cagctcctga atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag 3360
aggggcgggc tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca 3420
cggcagatca ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat 3480
gagaatgaca agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc 3540
gacttcagga aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc 3600
catgacgcgt acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc 3660
gagagcgagt tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag 3720
tcggagcagg agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat 3780
ttcttcaaga cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag 3840
acgaacggcg agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg 3900
aaggttctct ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg 3960
ttctctaagg agtcaattcg gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag 4020
gactgggatc cgaagaagta cggcgggttc gtcagcccca ctgtggccta ctcggtcctg 4080
gttgtggcga aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg 4140
gggatcacga ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg 4200
aagggctaca aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc 4260
gagctggaga acggcaggaa gcggatgctg gcttccgctc gcttcctgca gaaggggaac 4320
gagctggctc tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag 4380
ctcaagggca gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat 4440
tacctcgacg agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac 4500
gcgaatctgg ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag 4560
caggctgaga atatcattca tctcttcacc ctgacgaacc tcggcgcccc tcgtgctttc 4620
aagtacttcg acacaactat cgatcgcaag gtatacagaa gcactaagga ggtcctggac 4680
gcgaccctca tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag 4740
ctcgggggcg acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag 4800
gaattctccg gcggcagcac caacctgtcc gacatcatcg agaaggagac gggcaagcaa 4860
ctcgtgatcc aggagagcat cctcatgctg ccagaggagg tggaggaggt catcggcaac 4920
aagccagagt ccgacatcct ggtgcacacc gcctacgacg agtccaccga cgagaacgtc 4980
atgctcctga ccagcgacgc cccagagtac aagccatggg ccctcgtcat ccaggacagc 5040
aacggggaga acaagatcaa gatgctgtcg ggggggagcc caaagaagaa gcggaaggtg 5100
tag 5103
<210> 186
<211> 1700
<212> PRT
<213> Artificial Sequence
<220>
<223> A3A-PBE-NG
<400> 186
Met Glu Ala Ser Pro Ala Ser Gly Pro Arg His Leu Met Asp Pro His
1 5 10 15
Ile Phe Thr Ser Asn Phe Asn Asn Gly Ile Gly Arg His Lys Thr Tyr
20 25 30
Leu Cys Tyr Glu Val Glu Arg Leu Asp Asn Gly Thr Ser Val Lys Met
35 40 45
Asp Gln His Arg Gly Phe Leu His Asn Gln Ala Lys Asn Leu Leu Cys
50 55 60
Gly Phe Tyr Gly Arg His Ala Glu Leu Arg Phe Leu Asp Leu Val Pro
65 70 75 80
Ser Leu Gln Leu Asp Pro Ala Gln Ile Tyr Arg Val Thr Trp Phe Ile
85 90 95
Ser Trp Ser Pro Cys Phe Ser Trp Gly Cys Ala Gly Glu Val Arg Ala
100 105 110
Phe Leu Gln Glu Asn Thr His Val Arg Leu Arg Ile Phe Ala Ala Arg
115 120 125
Ile Tyr Asp Tyr Asp Pro Leu Tyr Lys Glu Ala Leu Gln Met Leu Arg
130 135 140
Asp Ala Gly Ala Gln Val Ser Ile Met Thr Tyr Asp Glu Phe Lys His
145 150 155 160
Cys Trp Asp Thr Phe Val Asp His Gln Gly Cys Pro Phe Gln Pro Trp
165 170 175
Asp Gly Leu Asp Glu His Ser Gln Ala Leu Ser Gly Arg Leu Arg Ala
180 185 190
Ile Leu Gln Asn Gln Gly Asn Ser Gly Ser Glu Thr Pro Gly Thr Ser
195 200 205
Glu Ser Ala Thr Pro Glu Ser Leu Lys Asp Lys Lys Tyr Ser Ile Gly
210 215 220
Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val Ile Thr Asp Glu
225 230 235 240
Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly Asn Thr Asp Arg
245 250 255
His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu Phe Asp Ser Gly
260 265 270
Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala Arg Arg Arg Tyr
275 280 285
Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu Ile Phe Ser Asn
290 295 300
Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg Leu Glu Glu Ser
305 310 315 320
Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His Pro Ile Phe Gly
325 330 335
Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr Pro Thr Ile Tyr
340 345 350
His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys Ala Asp Leu Arg
355 360 365
Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe Arg Gly His Phe
370 375 380
Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp Val Asp Lys Leu
385 390 395 400
Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe Glu Glu Asn Pro
405 410 415
Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu Ser Ala Arg Leu
420 425 430
Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln Leu Pro Gly Glu
435 440 445
Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu Ser Leu Gly Leu
450 455 460
Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu Asp Ala Lys Leu
465 470 475 480
Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp Asn Leu Leu Ala
485 490 495
Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala Ala Lys Asn Leu
500 505 510
Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val Asn Thr Glu Ile
515 520 525
Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg Tyr Asp Glu His
530 535 540
His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg Gln Gln Leu Pro
545 550 555 560
Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys Asn Gly Tyr Ala
565 570 575
Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe Tyr Lys Phe Ile
580 585 590
Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu Leu Leu Val Lys
595 600 605
Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr Phe Asp Asn Gly
610 615 620
Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His Ala Ile Leu Arg
625 630 635 640
Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn Arg Glu Lys Ile
645 650 655
Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val Gly Pro Leu Ala
660 665 670
Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys Ser Glu Glu Thr
675 680 685
Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys Gly Ala Ser Ala
690 695 700
Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys Asn Leu Pro Asn
705 710 715 720
Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu Tyr Phe Thr Val
725 730 735
Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu Gly Met Arg Lys
740 745 750
Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile Val Asp Leu Leu
755 760 765
Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu Lys Glu Asp Tyr
770 775 780
Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile Ser Gly Val Glu
785 790 795 800
Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp Leu Leu Lys Ile
805 810 815
Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn Glu Asp Ile Leu
820 825 830
Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu Asp Arg Glu Met Ile
835 840 845
Glu Glu Arg Leu Lys Thr Tyr Ala His Leu Phe Asp Asp Lys Val Met
850 855 860
Lys Gln Leu Lys Arg Arg Arg Tyr Thr Gly Trp Gly Arg Leu Ser Arg
865 870 875 880
Lys Leu Ile Asn Gly Ile Arg Asp Lys Gln Ser Gly Lys Thr Ile Leu
885 890 895
Asp Phe Leu Lys Ser Asp Gly Phe Ala Asn Arg Asn Phe Met Gln Leu
900 905 910
Ile His Asp Asp Ser Leu Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln
915 920 925
Val Ser Gly Gln Gly Asp Ser Leu His Glu His Ile Ala Asn Leu Ala
930 935 940
Gly Ser Pro Ala Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val
945 950 955 960
Asp Glu Leu Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val
965 970 975
Ile Glu Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn
980 985 990
Ser Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly
995 1000 1005
Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln
1010 1015 1020
Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met
1025 1030 1035
Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp
1040 1045 1050
Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile
1055 1060 1065
Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly Lys Ser
1070 1075 1080
Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn Tyr
1085 1090 1095
Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe
1100 1105 1110
Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp
1115 1120 1125
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile
1130 1135 1140
Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys
1145 1150 1155
Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr
1160 1165 1170
Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
1175 1180 1185
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala
1190 1195 1200
Tyr Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro
1205 1210 1215
Lys Leu Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp
1220 1225 1230
Val Arg Lys Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala
1235 1240 1245
Thr Ala Lys Tyr Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys
1250 1255 1260
Thr Glu Ile Thr Leu Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu
1265 1270 1275
Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile Val Trp Asp Lys Gly
1280 1285 1290
Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val
1295 1300 1305
Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys
1310 1315 1320
Glu Ser Ile Arg Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg
1325 1330 1335
Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Val Ser Pro
1340 1345 1350
Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly
1355 1360 1365
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr
1370 1375 1380
Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu
1385 1390 1395
Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys
1400 1405 1410
Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
1415 1420 1425
Met Leu Ala Ser Ala Arg Phe Leu Gln Lys Gly Asn Glu Leu Ala
1430 1435 1440
Leu Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr
1445 1450 1455
Glu Lys Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu
1460 1465 1470
Phe Val Glu Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln
1475 1480 1485
Ile Ser Glu Phe Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu
1490 1495 1500
Asp Lys Val Leu Ser Ala Tyr Asn Lys His Arg Asp Lys Pro Ile
1505 1510 1515
Arg Glu Gln Ala Glu Asn Ile Ile His Leu Phe Thr Leu Thr Asn
1520 1525 1530
Leu Gly Ala Pro Arg Ala Phe Lys Tyr Phe Asp Thr Thr Ile Asp
1535 1540 1545
Arg Lys Val Tyr Arg Ser Thr Lys Glu Val Leu Asp Ala Thr Leu
1550 1555 1560
Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile Asp Leu
1565 1570 1575
Ser Gln Leu Gly Gly Asp Lys Arg Pro Ala Ala Thr Lys Lys Ala
1580 1585 1590
Gly Gln Ala Lys Lys Lys Lys Glu Phe Ser Gly Gly Ser Thr Asn
1595 1600 1605
Leu Ser Asp Ile Ile Glu Lys Glu Thr Gly Lys Gln Leu Val Ile
1610 1615 1620
Gln Glu Ser Ile Leu Met Leu Pro Glu Glu Val Glu Glu Val Ile
1625 1630 1635
Gly Asn Lys Pro Glu Ser Asp Ile Leu Val His Thr Ala Tyr Asp
1640 1645 1650
Glu Ser Thr Asp Glu Asn Val Met Leu Leu Thr Ser Asp Ala Pro
1655 1660 1665
Glu Tyr Lys Pro Trp Ala Leu Val Ile Gln Asp Ser Asn Gly Glu
1670 1675 1680
Asn Lys Ile Lys Met Leu Ser Gly Gly Ser Pro Lys Lys Lys Arg
1685 1690 1695
Lys Val
1700
<210> 187
<211> 5394
<212> DNA
<213> Artificial Sequence
<220>
<223> PABE7-NG
<400> 187
atgtccgagg tggagttctc tcacgagtat tggatgaggc acgctcttac acttgctaag 60
agagcttggg acgaaagaga agtgccagtt ggcgccgttc ttgtgcataa taatagggtg 120
atcggcgagg gttggaatag accaattgga aggcatgatc caacagctca cgcagagatt 180
atggctctca gacaaggcgg cctcgttatg cagaactaca ggctcattga cgctacactc 240
tacgtgacac tcgaaccttg cgttatgtgc gccggagcta tgattcattc taggattggc 300
agggtcgtgt ttggagctag ggacgctaaa acaggagccg ccggatctct tatggacgtg 360
ttgcatcatc caggcatgaa ccatagggtg gagattacag agggcattct tgcagacgag 420
tgcgctgctc ttctttccga tttcttcagg atgagaaggc aggagattaa ggcccagaag 480
aaggctcagt cttctacaga tagcggagga tcttccggag gatctagcgg ctccgagaca 540
ccaggaacat ccgaaagcgc tacaccagaa tctagcggag gctcttccgg aggatcttct 600
gaagtggagt tctcccacga gtattggatg aggcacgctc ttacacttgc taaaagggct 660
agggacgaaa gggaagttcc agttggagct gttctcgtgc tcaataacag ggtgattggc 720
gagggttgga atagagccat tggactccat gatccaacag ctcacgcaga gattatggct 780
cttagacaag gcggcctcgt tatgcagaat tacagactca tcgacgccac actctacgtt 840
accttcgaac cttgcgttat gtgcgccgga gctatgatcc attctaggat tggcagggtc 900
gtgttcggcg ttagaaacgc taagacagga gctgcaggct ctcttatgga cgttcttcat 960
tacccaggca tgaatcatag agtggagatc acagaaggca ttcttgcaga cgagtgcgca 1020
gctctccttt gctatttctt caggatgccg aggcaagttt tcaacgctca gaagaaggcc 1080
cagtcttcta cagattccgg cggatcttct ggaggatcta gcggctccga gacaccagga 1140
acatccgaat ccgctacacc agagtcttct ggaggatcta gcggaggatc tgacaagaag 1200
tactcgatcg gcctcgccat tgggactaac tctgttggct gggccgtgat caccgacgag 1260
tacaaggtgc cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag 1320
aagaatctca ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc 1380
aagcgcaccg cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag 1440
attttctcca acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca 1500
ttcctcgtgg aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac 1560
gaggttgcct accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac 1620
tccacagata aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc 1680
aggggccatt tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg 1740
ttcatccagc tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca 1800
ggcgtcgacg cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac 1860
ctgatcgccc agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc 1920
agcctggggc tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg 1980
cagctctcca aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat 2040
cagtacgcgg acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat 2100
attctcaggg tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc 2160
tacgacgagc accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc 2220
gagaagtaca aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac 2280
ggcggggcct ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac 2340
ggcacggagg agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca 2400
ttcgataacg gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg 2460
aggcaggagg acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg 2520
actttcagga tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg 2580
atgacccgca agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag 2640
ggcgctagcg ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat 2700
gagaaggtgc tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg 2760
actaaggtga agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag 2820
aagaaggcca tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc 2880
aaggaggact acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag 2940
gaccgcttca acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag 3000
gacttcctgg acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact 3060
ctgttcgagg accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat 3120
gacaaggtca tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg 3180
aagctcatca acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag 3240
agcgatggct tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc 3300
aaggaggata tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc 3360
gcgaacctcg ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg 3420
gacgagctgg tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc 3480
cgggagaatc agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc 3540
gaggagggca ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg 3600
cagctgcaga atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg 3660
gaccaggagc tggatattaa caggctcagc gattacgacg tcgatcatat cgttccacag 3720
tcattcctga aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg 3780
ggcaagtctg ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc 3840
cagctcctga atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag 3900
aggggcgggc tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca 3960
cggcagatca ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat 4020
gagaatgaca agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc 4080
gacttcagga aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc 4140
catgacgcgt acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc 4200
gagagcgagt tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag 4260
tcggagcagg agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat 4320
ttcttcaaga cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag 4380
acgaacggcg agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg 4440
aaggttctct ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg 4500
ttctctaagg agtcaattcg gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag 4560
gactgggatc cgaagaagta cggcgggttc gtcagcccca ctgtggccta ctcggtcctg 4620
gttgtggcga aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg 4680
gggatcacga ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg 4740
aagggctaca aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc 4800
gagctggaga acggcaggaa gcggatgctg gcttccgctc gcttcctgca gaaggggaac 4860
gagctggctc tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag 4920
ctcaagggca gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat 4980
tacctcgacg agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac 5040
gcgaatctgg ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag 5100
caggctgaga atatcattca tctcttcacc ctgacgaacc tcggcgcccc tcgtgctttc 5160
aagtacttcg acacaactat cgatcgcaag gtatacagaa gcactaagga ggtcctggac 5220
gcgaccctca tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag 5280
ctcgggggcg actccggcgg cagcccaaag aagaagcgga aggtgtctgg aggttctcct 5340
aagaaaaaga gaaaagtgtc cggcggctcc ccgaagaaga agcgcaaggt gtag 5394
<210> 188
<211> 1797
<212> PRT
<213> Artificial Sequence
<220>
<223> PABE7-NG
<400> 188
Met Ser Glu Val Glu Phe Ser His Glu Tyr Trp Met Arg His Ala Leu
1 5 10 15
Thr Leu Ala Lys Arg Ala Trp Asp Glu Arg Glu Val Pro Val Gly Ala
20 25 30
Val Leu Val His Asn Asn Arg Val Ile Gly Glu Gly Trp Asn Arg Pro
35 40 45
Ile Gly Arg His Asp Pro Thr Ala His Ala Glu Ile Met Ala Leu Arg
50 55 60
Gln Gly Gly Leu Val Met Gln Asn Tyr Arg Leu Ile Asp Ala Thr Leu
65 70 75 80
Tyr Val Thr Leu Glu Pro Cys Val Met Cys Ala Gly Ala Met Ile His
85 90 95
Ser Arg Ile Gly Arg Val Val Phe Gly Ala Arg Asp Ala Lys Thr Gly
100 105 110
Ala Ala Gly Ser Leu Met Asp Val Leu His His Pro Gly Met Asn His
115 120 125
Arg Val Glu Ile Thr Glu Gly Ile Leu Ala Asp Glu Cys Ala Ala Leu
130 135 140
Leu Ser Asp Phe Phe Arg Met Arg Arg Gln Glu Ile Lys Ala Gln Lys
145 150 155 160
Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly Ser Ser Gly Gly Ser Ser
165 170 175
Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser Ala Thr Pro Glu Ser Ser
180 185 190
Gly Gly Ser Ser Gly Gly Ser Ser Glu Val Glu Phe Ser His Glu Tyr
195 200 205
Trp Met Arg His Ala Leu Thr Leu Ala Lys Arg Ala Arg Asp Glu Arg
210 215 220
Glu Val Pro Val Gly Ala Val Leu Val Leu Asn Asn Arg Val Ile Gly
225 230 235 240
Glu Gly Trp Asn Arg Ala Ile Gly Leu His Asp Pro Thr Ala His Ala
245 250 255
Glu Ile Met Ala Leu Arg Gln Gly Gly Leu Val Met Gln Asn Tyr Arg
260 265 270
Leu Ile Asp Ala Thr Leu Tyr Val Thr Phe Glu Pro Cys Val Met Cys
275 280 285
Ala Gly Ala Met Ile His Ser Arg Ile Gly Arg Val Val Phe Gly Val
290 295 300
Arg Asn Ala Lys Thr Gly Ala Ala Gly Ser Leu Met Asp Val Leu His
305 310 315 320
Tyr Pro Gly Met Asn His Arg Val Glu Ile Thr Glu Gly Ile Leu Ala
325 330 335
Asp Glu Cys Ala Ala Leu Leu Cys Tyr Phe Phe Arg Met Pro Arg Gln
340 345 350
Val Phe Asn Ala Gln Lys Lys Ala Gln Ser Ser Thr Asp Ser Gly Gly
355 360 365
Ser Ser Gly Gly Ser Ser Gly Ser Glu Thr Pro Gly Thr Ser Glu Ser
370 375 380
Ala Thr Pro Glu Ser Ser Gly Gly Ser Ser Gly Gly Ser Asp Lys Lys
385 390 395 400
Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val Gly Trp Ala Val
405 410 415
Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys Val Leu Gly
420 425 430
Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly Ala Leu Leu
435 440 445
Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys Arg Thr Ala
450 455 460
Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr Leu Gln Glu
465 470 475 480
Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe Phe His Arg
485 490 495
Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His Glu Arg His
500 505 510
Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His Glu Lys Tyr
515 520 525
Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser Thr Asp Lys
530 535 540
Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met Ile Lys Phe
545 550 555 560
Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp Asn Ser Asp
565 570 575
Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn Gln Leu Phe
580 585 590
Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys Ala Ile Leu
595 600 605
Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu Ile Ala Gln
610 615 620
Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu Ile Ala Leu
625 630 635 640
Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp Leu Ala Glu
645 650 655
Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp Asp Leu Asp
660 665 670
Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu Phe Leu Ala
675 680 685
Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile Leu Arg Val
690 695 700
Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met Ile Lys Arg
705 710 715 720
Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala Leu Val Arg
725 730 735
Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp Gln Ser Lys
740 745 750
Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln Glu Glu Phe
755 760 765
Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly Thr Glu Glu
770 775 780
Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys Gln Arg Thr
785 790 795 800
Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly Glu Leu His
805 810 815
Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu Lys Asp Asn
820 825 830
Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro Tyr Tyr Val
835 840 845
Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met Thr Arg Lys
850 855 860
Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val Val Asp Lys
865 870 875 880
Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn Phe Asp Lys
885 890 895
Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu Leu Tyr Glu
900 905 910
Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr Val Thr Glu
915 920 925
Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys Lys Ala Ile
930 935 940
Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val Lys Gln Leu
945 950 955 960
Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser Val Glu Ile
965 970 975
Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr Tyr His Asp
980 985 990
Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn Glu Glu Asn
995 1000 1005
Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu Phe Glu
1010 1015 1020
Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His Leu
1025 1030 1035
Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr
1040 1045 1050
Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp
1055 1060 1065
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly
1070 1075 1080
Phe Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu
1085 1090 1095
Thr Phe Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly
1100 1105 1110
Asp Ser Leu His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala
1115 1120 1125
Ile Lys Lys Gly Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu
1130 1135 1140
Val Lys Val Met Gly Arg His Lys Pro Glu Asn Ile Val Ile Glu
1145 1150 1155
Met Ala Arg Glu Asn Gln Thr Thr Gln Lys Gly Gln Lys Asn Ser
1160 1165 1170
Arg Glu Arg Met Lys Arg Ile Glu Glu Gly Ile Lys Glu Leu Gly
1175 1180 1185
Ser Gln Ile Leu Lys Glu His Pro Val Glu Asn Thr Gln Leu Gln
1190 1195 1200
Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln Asn Gly Arg Asp Met
1205 1210 1215
Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu Ser Asp Tyr Asp
1220 1225 1230
Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp Asp Ser Ile
1235 1240 1245
Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly Lys Ser
1250 1255 1260
Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn Tyr
1265 1270 1275
Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe
1280 1285 1290
Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp
1295 1300 1305
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile
1310 1315 1320
Thr Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys
1325 1330 1335
Tyr Asp Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr
1340 1345 1350
Leu Lys Ser Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe
1355 1360 1365
Tyr Lys Val Arg Glu Ile Asn Asn Tyr His His Ala His Asp Ala
1370 1375 1380
Tyr Leu Asn Ala Val Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro
1385 1390 1395
Lys Leu Glu Ser Glu Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp
1400 1405 1410
Val Arg Lys Met Ile Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala
1415 1420 1425
Thr Ala Lys Tyr Phe Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys
1430 1435 1440
Thr Glu Ile Thr Leu Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu
1445 1450 1455
Ile Glu Thr Asn Gly Glu Thr Gly Glu Ile Val Trp Asp Lys Gly
1460 1465 1470
Arg Asp Phe Ala Thr Val Arg Lys Val Leu Ser Met Pro Gln Val
1475 1480 1485
Asn Ile Val Lys Lys Thr Glu Val Gln Thr Gly Gly Phe Ser Lys
1490 1495 1500
Glu Ser Ile Arg Pro Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg
1505 1510 1515
Lys Lys Asp Trp Asp Pro Lys Lys Tyr Gly Gly Phe Val Ser Pro
1520 1525 1530
Thr Val Ala Tyr Ser Val Leu Val Val Ala Lys Val Glu Lys Gly
1535 1540 1545
Lys Ser Lys Lys Leu Lys Ser Val Lys Glu Leu Leu Gly Ile Thr
1550 1555 1560
Ile Met Glu Arg Ser Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu
1565 1570 1575
Glu Ala Lys Gly Tyr Lys Glu Val Lys Lys Asp Leu Ile Ile Lys
1580 1585 1590
Leu Pro Lys Tyr Ser Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg
1595 1600 1605
Met Leu Ala Ser Ala Arg Phe Leu Gln Lys Gly Asn Glu Leu Ala
1610 1615 1620
Leu Pro Ser Lys Tyr Val Asn Phe Leu Tyr Leu Ala Ser His Tyr
1625 1630 1635
Glu Lys Leu Lys Gly Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu
1640 1645 1650
Phe Val Glu Gln His Lys His Tyr Leu Asp Glu Ile Ile Glu Gln
1655 1660 1665
Ile Ser Glu Phe Ser Lys Arg Val Ile Leu Ala Asp Ala Asn Leu
1670 1675 1680
Asp Lys Val Leu Ser Ala Tyr Asn Lys His Arg Asp Lys Pro Ile
1685 1690 1695
Arg Glu Gln Ala Glu Asn Ile Ile His Leu Phe Thr Leu Thr Asn
1700 1705 1710
Leu Gly Ala Pro Arg Ala Phe Lys Tyr Phe Asp Thr Thr Ile Asp
1715 1720 1725
Arg Lys Val Tyr Arg Ser Thr Lys Glu Val Leu Asp Ala Thr Leu
1730 1735 1740
Ile His Gln Ser Ile Thr Gly Leu Tyr Glu Thr Arg Ile Asp Leu
1745 1750 1755
Ser Gln Leu Gly Gly Asp Ser Gly Gly Ser Pro Lys Lys Lys Arg
1760 1765 1770
Lys Val Ser Gly Gly Ser Pro Lys Lys Lys Arg Lys Val Ser Gly
1775 1780 1785
Gly Ser Pro Lys Lys Lys Arg Lys Val
1790 1795
<210> 189
<211> 4203
<212> DNA
<213> Artificial Sequence
<220>
<223> pCas9-NG
<400> 189
atggctccga agaagaagag gaaggttggc atccacgggg tgccagctgc tgacaagaag 60
tactcgatcg gcctcgccat tgggactaac tctgttggct gggccgtgat caccgacgag 120
tacaaggtgc cctcaaagaa gttcaaggtc ctgggcaaca ccgatcggca ttccatcaag 180
aagaatctca ttggcgctct cctgttcgac agcggcgaga cggctgaggc tacgcggctc 240
aagcgcaccg cccgcaggcg gtacacgcgc aggaagaatc gcatctgcta cctgcaggag 300
attttctcca acgagatggc gaaggttgac gattctttct tccacaggct ggaggagtca 360
ttcctcgtgg aggaggataa gaagcacgag cggcatccaa tcttcggcaa cattgtcgac 420
gaggttgcct accacgagaa gtaccctacg atctaccatc tgcggaagaa gctcgtggac 480
tccacagata aggcggacct ccgcctgatc tacctcgctc tggcccacat gattaagttc 540
aggggccatt tcctgatcga gggggatctc aacccggaca atagcgatgt tgacaagctg 600
ttcatccagc tcgtgcagac gtacaaccag ctcttcgagg agaaccccat taatgcgtca 660
ggcgtcgacg cgaaggctat cctgtccgct aggctctcga agtctcggcg cctcgagaac 720
ctgatcgccc agctgccggg cgagaagaag aacggcctgt tcgggaatct cattgcgctc 780
agcctggggc tcacgcccaa cttcaagtcg aatttcgatc tcgctgagga cgccaagctg 840
cagctctcca aggacacata cgacgatgac ctggataacc tcctggccca gatcggcgat 900
cagtacgcgg acctgttcct cgctgccaag aatctgtcgg acgccatcct cctgtctgat 960
attctcaggg tgaacaccga gattacgaag gctccgctct cagcctccat gatcaagcgc 1020
tacgacgagc accatcagga tctgaccctc ctgaaggcgc tggtcaggca gcagctcccc 1080
gagaagtaca aggagatctt cttcgatcag tcgaagaacg gctacgctgg gtacattgac 1140
ggcggggcct ctcaggagga gttctacaag ttcatcaagc cgattctgga gaagatggac 1200
ggcacggagg agctgctggt gaagctcaat cgcgaggacc tcctgaggaa gcagcggaca 1260
ttcgataacg gcagcatccc acaccagatt catctcgggg agctgcacgc tatcctgagg 1320
aggcaggagg acttctaccc tttcctcaag gataaccgcg agaagatcga gaagattctg 1380
actttcagga tcccgtacta cgtcggccca ctcgctaggg gcaactcccg cttcgcttgg 1440
atgacccgca agtcagagga gacgatcacg ccgtggaact tcgaggaggt ggtcgacaag 1500
ggcgctagcg ctcagtcgtt catcgagagg atgacgaatt tcgacaagaa cctgccaaat 1560
gagaaggtgc tccctaagca ctcgctcctg tacgagtact tcacagtcta caacgagctg 1620
actaaggtga agtatgtgac cgagggcatg aggaagccgg ctttcctgtc tggggagcag 1680
aagaaggcca tcgtggacct cctgttcaag accaaccgga aggtcacggt taagcagctc 1740
aaggaggact acttcaagaa gattgagtgc ttcgattcgg tcgagatctc tggcgttgag 1800
gaccgcttca acgcctccct ggggacctac cacgatctcc tgaagatcat taaggataag 1860
gacttcctgg acaacgagga gaatgaggat atcctcgagg acattgtgct gacactcact 1920
ctgttcgagg accgggagat gatcgaggag cgcctgaaga cttacgccca tctcttcgat 1980
gacaaggtca tgaagcagct caagaggagg aggtacaccg gctgggggag gctgagcagg 2040
aagctcatca acggcattcg ggacaagcag tccgggaaga cgatcctcga cttcctgaag 2100
agcgatggct tcgcgaaccg caatttcatg cagctgattc acgatgacag cctcacattc 2160
aaggaggata tccagaaggc tcaggtgagc ggccaggggg actcgctgca cgagcatatc 2220
gcgaacctcg ctggctcgcc agctatcaag aaggggattc tgcagaccgt gaaggttgtg 2280
gacgagctgg tgaaggtcat gggcaggcac aagcctgaga acatcgtcat tgagatggcc 2340
cgggagaatc agaccacgca gaagggccag aagaactcac gcgagaggat gaagaggatc 2400
gaggagggca ttaaggagct ggggtcccag atcctcaagg agcacccggt ggagaacacg 2460
cagctgcaga atgagaagct ctacctgtac tacctccaga atggccgcga tatgtatgtg 2520
gaccaggagc tggatattaa caggctcagc gattacgacg tcgatcatat cgttccacag 2580
tcattcctga aggatgactc cattgacaac aaggtcctca ccaggtcgga caagaaccgg 2640
ggcaagtctg ataatgttcc ttcagaggag gtcgttaaga agatgaagaa ctactggcgc 2700
cagctcctga atgccaagct gatcacgcag cggaagttcg ataacctcac aaaggctgag 2760
aggggcgggc tctctgagct ggacaaggcg ggcttcatca agaggcagct ggtcgagaca 2820
cggcagatca ctaagcacgt tgcgcagatt ctcgactcac ggatgaacac taagtacgat 2880
gagaatgaca agctgatccg cgaggtgaag gtcatcaccc tgaagtcaaa gctcgtctcc 2940
gacttcagga aggatttcca gttctacaag gttcgggaga tcaacaatta ccaccatgcc 3000
catgacgcgt acctgaacgc ggtggtcggc acagctctga tcaagaagta cccaaagctc 3060
gagagcgagt tcgtgtacgg ggactacaag gtttacgatg tgaggaagat gatcgccaag 3120
tcggagcagg agattggcaa ggctaccgcc aagtacttct tctactctaa cattatgaat 3180
ttcttcaaga cagagatcac tctggccaat ggcgagatcc ggaagcgccc cctcatcgag 3240
acgaacggcg agacggggga gatcgtgtgg gacaagggca gggatttcgc gaccgtcagg 3300
aaggttctct ccatgccaca agtgaatatc gtcaagaaga cagaggtcca gactggcggg 3360
ttctctaagg agtcaattcg gcctaagcgg aacagcgaca agctcatcgc ccgcaagaag 3420
gactgggatc cgaagaagta cggcgggttc gtcagcccca ctgtggccta ctcggtcctg 3480
gttgtggcga aggttgagaa gggcaagtcc aagaagctca agagcgtgaa ggagctgctg 3540
gggatcacga ttatggagcg ctccagcttc gagaagaacc cgatcgattt cctggaggcg 3600
aagggctaca aggaggtgaa gaaggacctg atcattaagc tccccaagta ctcactcttc 3660
gagctggaga acggcaggaa gcggatgctg gcttccgctc gcttcctgca gaaggggaac 3720
gagctggctc tgccgtccaa gtatgtgaac ttcctctacc tggcctccca ctacgagaag 3780
ctcaagggca gccccgagga caacgagcag aagcagctgt tcgtcgagca gcacaagcat 3840
tacctcgacg agatcattga gcagatttcc gagttctcca agcgcgtgat cctggccgac 3900
gcgaatctgg ataaggtcct ctccgcgtac aacaagcacc gcgacaagcc aatcagggag 3960
caggctgaga atatcattca tctcttcacc ctgacgaacc tcggcgcccc tcgtgctttc 4020
aagtacttcg acacaactat cgatcgcaag gtatacagaa gcactaagga ggtcctggac 4080
gcgaccctca tccaccagtc gattaccggc ctctacgaga cgcgcatcga cctgtctcag 4140
ctcgggggcg acaagcggcc agcggcgacg aagaaggcgg ggcaggcgaa gaagaagaag 4200
tag 4203
<210> 190
<211> 1400
<212> PRT
<213> Artificial Sequence
<220>
<223> pCas9-NG
<400> 190
Met Ala Pro Lys Lys Lys Arg Lys Val Gly Ile His Gly Val Pro Ala
1 5 10 15
Ala Asp Lys Lys Tyr Ser Ile Gly Leu Ala Ile Gly Thr Asn Ser Val
20 25 30
Gly Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe
35 40 45
Lys Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile
50 55 60
Gly Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu
65 70 75 80
Lys Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys
85 90 95
Tyr Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser
100 105 110
Phe Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys
115 120 125
His Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr
130 135 140
His Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp
145 150 155 160
Ser Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His
165 170 175
Met Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro
180 185 190
Asp Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr
195 200 205
Asn Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala
210 215 220
Lys Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn
225 230 235 240
Leu Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn
245 250 255
Leu Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe
260 265 270
Asp Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp
275 280 285
Asp Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp
290 295 300
Leu Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp
305 310 315 320
Ile Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser
325 330 335
Met Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys
340 345 350
Ala Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe
355 360 365
Asp Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser
370 375 380
Gln Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp
385 390 395 400
Gly Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg
405 410 415
Lys Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu
420 425 430
Gly Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe
435 440 445
Leu Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile
450 455 460
Pro Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp
465 470 475 480
Met Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu
485 490 495
Val Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr
500 505 510
Asn Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser
515 520 525
Leu Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys
530 535 540
Tyr Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln
545 550 555 560
Lys Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr
565 570 575
Val Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp
580 585 590
Ser Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly
595 600 605
Thr Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp
610 615 620
Asn Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr
625 630 635 640
Leu Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala
645 650 655
His Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr
660 665 670
Thr Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp
675 680 685
Lys Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe
690 695 700
Ala Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe
705 710 715 720
Lys Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu
725 730 735
His Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly
740 745 750
Ile Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly
755 760 765
Arg His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln
770 775 780
Thr Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile
785 790 795 800
Glu Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro
805 810 815
Val Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu
820 825 830
Gln Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg
835 840 845
Leu Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys
850 855 860
Asp Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg
865 870 875 880
Gly Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys
885 890 895
Asn Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys
900 905 910
Phe Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp
915 920 925
Lys Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr
930 935 940
Lys His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp
945 950 955 960
Glu Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser
965 970 975
Lys Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg
980 985 990
Glu Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val
995 1000 1005
Val Gly Thr Ala Leu Ile Lys Lys Tyr Pro Lys Leu Glu Ser Glu
1010 1015 1020
Phe Val Tyr Gly Asp Tyr Lys Val Tyr Asp Val Arg Lys Met Ile
1025 1030 1035
Ala Lys Ser Glu Gln Glu Ile Gly Lys Ala Thr Ala Lys Tyr Phe
1040 1045 1050
Phe Tyr Ser Asn Ile Met Asn Phe Phe Lys Thr Glu Ile Thr Leu
1055 1060 1065
Ala Asn Gly Glu Ile Arg Lys Arg Pro Leu Ile Glu Thr Asn Gly
1070 1075 1080
Glu Thr Gly Glu Ile Val Trp Asp Lys Gly Arg Asp Phe Ala Thr
1085 1090 1095
Val Arg Lys Val Leu Ser Met Pro Gln Val Asn Ile Val Lys Lys
1100 1105 1110
Thr Glu Val Gln Thr Gly Gly Phe Ser Lys Glu Ser Ile Arg Pro
1115 1120 1125
Lys Arg Asn Ser Asp Lys Leu Ile Ala Arg Lys Lys Asp Trp Asp
1130 1135 1140
Pro Lys Lys Tyr Gly Gly Phe Val Ser Pro Thr Val Ala Tyr Ser
1145 1150 1155
Val Leu Val Val Ala Lys Val Glu Lys Gly Lys Ser Lys Lys Leu
1160 1165 1170
Lys Ser Val Lys Glu Leu Leu Gly Ile Thr Ile Met Glu Arg Ser
1175 1180 1185
Ser Phe Glu Lys Asn Pro Ile Asp Phe Leu Glu Ala Lys Gly Tyr
1190 1195 1200
Lys Glu Val Lys Lys Asp Leu Ile Ile Lys Leu Pro Lys Tyr Ser
1205 1210 1215
Leu Phe Glu Leu Glu Asn Gly Arg Lys Arg Met Leu Ala Ser Ala
1220 1225 1230
Arg Phe Leu Gln Lys Gly Asn Glu Leu Ala Leu Pro Ser Lys Tyr
1235 1240 1245
Val Asn Phe Leu Tyr Leu Ala Ser His Tyr Glu Lys Leu Lys Gly
1250 1255 1260
Ser Pro Glu Asp Asn Glu Gln Lys Gln Leu Phe Val Glu Gln His
1265 1270 1275
Lys His Tyr Leu Asp Glu Ile Ile Glu Gln Ile Ser Glu Phe Ser
1280 1285 1290
Lys Arg Val Ile Leu Ala Asp Ala Asn Leu Asp Lys Val Leu Ser
1295 1300 1305
Ala Tyr Asn Lys His Arg Asp Lys Pro Ile Arg Glu Gln Ala Glu
1310 1315 1320
Asn Ile Ile His Leu Phe Thr Leu Thr Asn Leu Gly Ala Pro Arg
1325 1330 1335
Ala Phe Lys Tyr Phe Asp Thr Thr Ile Asp Arg Lys Val Tyr Arg
1340 1345 1350
Ser Thr Lys Glu Val Leu Asp Ala Thr Leu Ile His Gln Ser Ile
1355 1360 1365
Thr Gly Leu Tyr Glu Thr Arg Ile Asp Leu Ser Gln Leu Gly Gly
1370 1375 1380
Asp Lys Arg Pro Ala Ala Thr Lys Lys Ala Gly Gln Ala Lys Lys
1385 1390 1395
Lys Lys
1400
<210> 191
<211> 552
<212> PRT
<213> Oryza sativa
<400> 191
Pro Glu Phe Pro Ser Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile
1 5 10 15
Thr Phe Arg Ala Gly Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu
20 25 30
Ala Val Thr Asn Leu Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu
35 40 45
Ala Ala Asn Ser Gly Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser
50 55 60
Cys Phe Arg Val Gly Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe
65 70 75 80
Gln Tyr Ile Tyr Leu Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser
85 90 95
Val Ile Ala His Lys Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val
100 105 110
Ile Asp Ser Val Val Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile
115 120 125
His Gly Ser Ala Ala Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu
130 135 140
Thr Phe Thr Leu Thr Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala
145 150 155 160
Tyr Leu Ala Arg Leu Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro
165 170 175
Ile Ile Leu Thr Gly Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu
180 185 190
Val Tyr Ser Ser His Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr
195 200 205
Asn Gly Val Val His Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser
210 215 220
Asn Ile Leu Arg Trp Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro
225 230 235 240
Leu Pro Val Thr Thr Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr
245 250 255
Ile Pro Glu Asn Ser Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp
260 265 270
Asp Ser Gln Gly Lys Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe
275 280 285
Val Glu Thr Phe Glu Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala
290 295 300
Lys Leu Gly Gly Ile Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr
305 310 315 320
Met Met Gln Thr Ile Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu
325 330 335
Gln Ser Val Pro Arg Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr
340 345 350
Lys Thr Ala Gln Ala Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu
355 360 365
Phe Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu
370 375 380
Phe Glu Gly Ile Leu Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg
385 390 395 400
Thr Tyr Asn Gln Pro Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu
405 410 415
Arg Gly Gly Ala Trp Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg
420 425 430
Ile Glu Cys Tyr Ala Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro
435 440 445
Gln Gly Leu Ile Glu Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys
450 455 460
Met Ser Arg Leu Asp Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu
465 470 475 480
Val Ala Asn Lys Asn Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn
485 490 495
Ile Glu Ala Arg Thr Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala
500 505 510
Ile Arg Phe Ala Glu Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys
515 520 525
Gly Val Ile Lys Lys Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe
530 535 540
Tyr Lys Arg Leu Arg Arg Arg Ile
545 550
<210> 192
<211> 770
<212> PRT
<213> Oryza sativa
<400> 192
Gly Pro Leu His Gly Val Ala Leu Asn Asn Pro Tyr Gln Pro Leu Ser
1 5 10 15
Val Ile Asp Leu Lys Arg Cys Ser Ala Arg Asn Asn Arg Thr Thr Tyr
20 25 30
Cys Tyr Asp Phe Pro Leu Ala Phe Glu Thr Ala Val Arg Lys Ser Trp
35 40 45
Ser Ser Ser Thr Ser Gly Ala Ser Lys Gly Val Glu Asn Ala Gln Cys
50 55 60
Tyr Val Lys Ala Thr Glu Leu Val Phe Ala Asp Lys His Gly Ser Trp
65 70 75 80
Gly Thr Pro Leu Val Gln Met Asp Arg Pro Ala Gly Leu Asn Asp Ile
85 90 95
Gly Met Val Ala Trp Thr Leu Lys Met Ser Thr Pro Glu Phe Pro Ser
100 105 110
Gly Arg Glu Ile Ile Val Val Ala Asn Asp Ile Thr Phe Arg Ala Gly
115 120 125
Ser Phe Gly Pro Arg Glu Asp Ala Phe Phe Glu Ala Val Thr Asn Leu
130 135 140
Ala Cys Glu Lys Lys Leu Pro Leu Ile Tyr Leu Ala Ala Asn Ser Gly
145 150 155 160
Ala Arg Ile Gly Ile Ala Asp Glu Val Lys Ser Cys Phe Arg Val Gly
165 170 175
Trp Ser Asp Asp Gly Ser Pro Glu Arg Gly Phe Gln Tyr Ile Tyr Leu
180 185 190
Ser Glu Glu Asp Tyr Ala Arg Ile Gly Thr Ser Val Ile Ala His Lys
195 200 205
Met Gln Leu Asp Ser Gly Glu Ile Arg Trp Val Ile Asp Ser Val Val
210 215 220
Gly Lys Glu Asp Gly Leu Gly Val Glu Asn Ile His Gly Ser Ala Ala
225 230 235 240
Ile Ala Ser Ala Tyr Ser Arg Ala Tyr Lys Glu Thr Phe Thr Leu Thr
245 250 255
Phe Val Thr Gly Arg Thr Val Gly Ile Gly Ala Tyr Leu Ala Arg Leu
260 265 270
Gly Ile Arg Cys Ile Gln Arg Leu Asp Gln Pro Ile Ile Leu Thr Gly
275 280 285
Tyr Ser Ala Leu Asn Lys Leu Leu Gly Arg Glu Val Tyr Ser Ser His
290 295 300
Met Gln Leu Gly Gly Pro Lys Ile Met Ala Thr Asn Gly Val Val His
305 310 315 320
Leu Thr Val Ser Asp Asp Leu Glu Gly Val Ser Asn Ile Leu Arg Trp
325 330 335
Leu Ser Tyr Val Pro Ala Tyr Ile Gly Gly Pro Leu Pro Val Thr Thr
340 345 350
Pro Leu Asp Pro Pro Asp Arg Pro Val Ala Tyr Ile Pro Glu Asn Ser
355 360 365
Cys Asp Pro Arg Ala Ala Ile Arg Gly Val Asp Asp Ser Gln Gly Lys
370 375 380
Trp Leu Gly Gly Met Phe Asp Lys Asp Ser Phe Val Glu Thr Phe Glu
385 390 395 400
Gly Trp Ala Lys Thr Val Val Thr Gly Arg Ala Lys Leu Gly Gly Ile
405 410 415
Pro Val Gly Val Ile Ala Val Glu Thr Gln Thr Met Met Gln Thr Ile
420 425 430
Pro Ala Asp Pro Gly Gln Leu Asp Ser Arg Glu Gln Ser Val Pro Arg
435 440 445
Ala Gly Gln Val Trp Phe Pro Asp Ser Ala Thr Lys Thr Ala Gln Ala
450 455 460
Leu Leu Asp Phe Asn Arg Glu Gly Leu Pro Leu Phe Ile Leu Ala Asn
465 470 475 480
Trp Arg Gly Phe Ser Gly Gly Gln Arg Asp Leu Phe Glu Gly Ile Leu
485 490 495
Gln Ala Gly Ser Thr Ile Val Glu Asn Leu Arg Thr Tyr Asn Gln Pro
500 505 510
Ala Phe Val Tyr Ile Pro Met Ala Ala Glu Leu Arg Gly Gly Ala Trp
515 520 525
Val Val Val Asp Ser Lys Ile Asn Pro Asp Arg Ile Glu Cys Tyr Ala
530 535 540
Glu Arg Thr Ala Lys Gly Asn Val Leu Glu Pro Gln Gly Leu Ile Glu
545 550 555 560
Ile Lys Phe Arg Ser Glu Glu Leu Gln Asp Cys Met Ser Arg Leu Asp
565 570 575
Pro Thr Leu Ile Asp Leu Lys Ala Lys Leu Glu Val Ala Asn Lys Asn
580 585 590
Gly Ser Ala Asp Thr Lys Ser Leu Gln Glu Asn Ile Glu Ala Arg Thr
595 600 605
Lys Gln Leu Met Pro Leu Tyr Thr Gln Ile Ala Ile Arg Phe Ala Glu
610 615 620
Leu His Asp Thr Ser Leu Arg Met Ala Ala Lys Gly Val Ile Lys Lys
625 630 635 640
Val Val Asp Trp Glu Glu Ser Arg Ser Phe Phe Tyr Lys Arg Leu Arg
645 650 655
Arg Arg Ile Ser Glu Asp Val Leu Ala Lys Glu Ile Arg Ala Val Ala
660 665 670
Gly Glu Gln Phe Ser His Gln Pro Ala Ile Glu Leu Ile Lys Lys Trp
675 680 685
Tyr Ser Ala Ser His Ala Ala Glu Trp Asp Asp Asp Asp Ala Phe Val
690 695 700
Ala Trp Met Asp Asn Pro Glu Asn Tyr Lys Asp Tyr Ile Gln Tyr Leu
705 710 715 720
Lys Ala Gln Arg Val Ser Gln Ser Leu Ser Ser Leu Ser Asp Ser Ser
725 730 735
Ser Asp Leu Gln Ala Leu Pro Gln Gly Leu Ser Met Leu Leu Asp Lys
740 745 750
Met Asp Pro Ser Arg Arg Ala Gln Leu Val Glu Glu Ile Arg Lys Val
755 760 765
Leu Gly
770
<210> 193
<211> 737
<212> PRT
<213> Saccharomyces cerevisiae
<400> 193
Pro Ile Ala Thr Pro Tyr Pro Val Lys Glu Trp Leu Gln Pro Lys Arg
1 5 10 15
Tyr Lys Ala His Leu Met Gly Thr Thr Tyr Val Tyr Asp Phe Pro Glu
20 25 30
Leu Phe Arg Gln Ala Ser Ser Ser Gln Trp Lys Asn Phe Ser Ala Asp
35 40 45
Val Lys Leu Thr Asp Asp Phe Phe Ile Ser Asn Glu Leu Ile Glu Asp
50 55 60
Glu Asn Gly Glu Leu Thr Glu Val Glu Arg Glu Pro Gly Ala Asn Ala
65 70 75 80
Ile Gly Met Val Ala Phe Lys Ile Thr Val Lys Thr Pro Glu Tyr Pro
85 90 95
Arg Gly Arg Gln Phe Val Val Val Ala Asn Asp Ile Thr Phe Lys Ile
100 105 110
Gly Ser Phe Gly Pro Gln Glu Asp Glu Phe Phe Asn Lys Val Thr Glu
115 120 125
Tyr Ala Arg Lys Arg Gly Ile Pro Arg Ile Tyr Leu Ala Ala Asn Ser
130 135 140
Gly Ala Arg Ile Gly Met Ala Glu Glu Ile Val Pro Leu Phe Gln Val
145 150 155 160
Ala Trp Asn Asp Ala Ala Asn Pro Asp Lys Gly Phe Gln Tyr Leu Tyr
165 170 175
Leu Thr Ser Glu Gly Met Glu Thr Leu Lys Lys Phe Asp Lys Glu Asn
180 185 190
Ser Val Leu Thr Glu Arg Thr Val Ile Asn Gly Glu Glu Arg Phe Val
195 200 205
Ile Lys Thr Ile Ile Gly Ser Glu Asp Gly Leu Gly Val Glu Cys Leu
210 215 220
Arg Gly Ser Gly Leu Ile Ala Gly Ala Thr Ser Arg Ala Tyr His Asp
225 230 235 240
Ile Phe Thr Ile Thr Leu Val Thr Cys Arg Ser Val Gly Ile Gly Ala
245 250 255
Tyr Leu Val Arg Leu Gly Gln Arg Ala Ile Gln Val Glu Gly Gln Pro
260 265 270
Ile Ile Leu Thr Gly Ala Pro Ala Ile Asn Lys Met Leu Gly Arg Glu
275 280 285
Val Tyr Thr Ser Asn Leu Gln Leu Gly Gly Thr Gln Ile Met Tyr Asn
290 295 300
Asn Gly Val Ser His Leu Thr Ala Val Asp Asp Leu Ala Gly Val Glu
305 310 315 320
Lys Ile Val Glu Trp Met Ser Tyr Val Pro Ala Lys Arg Asn Met Pro
325 330 335
Val Pro Ile Leu Glu Thr Lys Asp Thr Trp Asp Arg Pro Val Asp Phe
340 345 350
Thr Pro Thr Asn Asp Glu Thr Tyr Asp Val Arg Trp Met Ile Glu Gly
355 360 365
Arg Glu Thr Glu Ser Gly Phe Glu Tyr Gly Leu Phe Asp Lys Gly Ser
370 375 380
Phe Phe Glu Thr Leu Ser Gly Trp Ala Lys Gly Val Val Val Gly Arg
385 390 395 400
Ala Arg Leu Gly Gly Ile Pro Leu Gly Val Ile Gly Val Glu Thr Arg
405 410 415
Thr Val Glu Asn Leu Ile Pro Ala Asp Pro Ala Asn Pro Asn Ser Ala
420 425 430
Glu Thr Leu Ile Gln Glu Pro Gly Gln Val Trp His Pro Asn Ser Ala
435 440 445
Phe Lys Thr Ala Gln Ala Ile Asn Asp Phe Asn Asn Gly Glu Gln Leu
450 455 460
Pro Met Met Ile Leu Ala Asn Trp Arg Gly Phe Ser Gly Gly Gln Arg
465 470 475 480
Asp Met Phe Asn Glu Val Leu Lys Tyr Gly Ser Phe Ile Val Asp Ala
485 490 495
Leu Val Asp Tyr Lys Gln Pro Ile Ile Ile Tyr Ile Pro Pro Thr Gly
500 505 510
Glu Leu Arg Gly Gly Ser Trp Val Val Val Asp Pro Thr Ile Asn Ala
515 520 525
Asp Gln Met Glu Met Tyr Ala Asp Val Asn Ala Arg Ala Gly Val Leu
530 535 540
Glu Pro Gln Gly Met Val Gly Ile Lys Phe Arg Arg Glu Lys Leu Leu
545 550 555 560
Asp Thr Met Asn Arg Leu Asp Asp Lys Tyr Arg Glu Leu Arg Ser Gln
565 570 575
Leu Ser Asn Lys Ser Leu Ala Pro Glu Val His Gln Gln Ile Ser Lys
580 585 590
Gln Leu Ala Asp Arg Glu Arg Glu Leu Leu Pro Ile Tyr Gly Gln Ile
595 600 605
Ser Leu Gln Phe Ala Asp Leu His Asp Arg Ser Ser Arg Met Val Ala
610 615 620
Lys Gly Val Ile Ser Lys Glu Leu Glu Trp Thr Glu Ala Arg Arg Phe
625 630 635 640
Phe Phe Trp Arg Leu Arg Arg Arg Leu Asn Glu Glu Tyr Leu Ile Lys
645 650 655
Arg Leu Ser His Gln Val Gly Glu Ala Ser Arg Leu Glu Lys Ile Ala
660 665 670
Arg Ile Arg Ser Trp Tyr Pro Ala Ser Val Asp His Glu Asp Asp Arg
675 680 685
Gln Val Ala Thr Trp Ile Glu Glu Asn Tyr Lys Thr Leu Asp Asp Lys
690 695 700
Leu Lys Gly Leu Lys Leu Glu Ser Phe Ala Gln Asp Leu Ala Lys Lys
705 710 715 720
Ile Arg Ser Asp His Asp Asn Ala Ile Asp Gly Leu Ser Glu Val Ile
725 730 735
Lys

Claims (20)

1.一种鉴定细胞系统中农艺学重要表型的方法，包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统；
(d)获得在至少一种感兴趣的核酸序列中包含至少一种修饰的细胞系统；
(e)在获得细胞系统M0群体的条件下培养细胞系统；
(f)筛选细胞系统的M0群体中与至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型；和
(g)鉴定并选择细胞系统中农艺学上重要的表型，
其中至少一种碱基编辑复合物的向导RNA阵列包含至少两种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
2.一种鉴定细胞系统中重要农艺表型的方法，包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣核酸序列的向导RNA阵列或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统的遗传物质中；
(d)在获得细胞系统M0群体的条件下培养细胞系统；
(e)将细胞系统的M0群体与包含至少一种感兴趣的核酸序列的细胞系统的野生型群体杂交以获得细胞系统的后代群体；
(f)获得在至少一种感兴趣的核酸序列中具有至少一种修饰的细胞系统的后代群体；
(g)针对与至少一种目的核酸中的至少一种修饰相关的农艺学重要表型筛选细胞系统的后代群体；和
(h)鉴定并选择细胞系统中农艺学上重要的表型，
其中至少一种碱基编辑复合物的向导RNA阵列包含至少两种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
3.一种产生具有重要农艺表型的修饰的细胞系统的方法，该方法包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统；
(d)获得在至少一种感兴趣的核酸序列中包含至少一种修饰的细胞系统；
(e)在获得细胞系统M0群体的条件下培养细胞系统；
(f)筛选细胞系统的M0群体中与至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型；和
(g)从具有重要农艺学表型的M0群体中鉴定并从而选择细胞系统；和
(h)获得具有重要农艺表型的修饰的细胞系统，
其中至少一种碱基编辑复合物的向导RNA阵列包含至少两种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
4.一种产生具有重要农艺表型的修饰的细胞系统后代的方法，该方法包括以下步骤：
(a)在细胞系统的遗传物质中选择至少一种感兴趣的核酸序列；
(b)提供至少一种碱基编辑器复合物或编码其的序列，其中所述至少一种碱基编辑器复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；或提供至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列，其中所述至少一种STEME复合物包含靶向至少一种感兴趣的核酸序列的向导RNA阵列或编码其的序列；
(c)将至少一种碱基编辑器复合物或编码其的序列、或至少一种饱和的靶向内源诱变编辑器(STEME)复合物或编码其的序列引入细胞系统的遗传物质中；
(d)在获得细胞系统M0群体的条件下培养细胞系统；
(e)将细胞系统的M0群体与包含至少一种目的核酸序列的细胞系统的野生型群体杂交以获得细胞系统的后代群体；
(f)获得在至少一种感兴趣的核酸序列中具有至少一种修饰的细胞系统的后代群体；
(g)针对与至少一种目的核酸中的至少一种修饰相关的农艺学重要表型筛选细胞系统的后代群体；和
(h)从具有重要农艺表型的后代群体中鉴定并由此选择细胞系统，
(i)获得具有重要农艺表型的修饰的细胞系统的后代，
其中至少一种碱基编辑复合物的向导RNA阵列包含至少两种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列；和
其中至少一种STEME复合物的向导RNA阵列包含至少一种向导RNA分子或编码其的序列，其靶向至少一种感兴趣的核酸序列。
5.根据前述权利要求中任一项所述的方法，其中所述向导RNA阵列包含至少两种、至少三种、至少四种、至少五种、至少六种、至少七种、至少八种、至少九、至少十、至少十一、至少十二、至少十三、至少十四、至少十五、至少十六、至少十七、至少十八、至少十九、至少二十或更多种靶向至少一种感兴趣的核酸序列的RNA分子。
6.根据权利要求5所述的方法，其中所述向导RNA分子靶向所述感兴趣核酸序列的重叠的和/或不同片段。
7.根据前述权利要求中任一项所述的方法，其中将所述至少一种碱基编辑器复合物或其组分或所述至少一种STEME复合物或其组分作为至少一种质粒、至少一种载体或至少一种线性DNA分子的一部分，作为RNA分子和/或作为RNA和/或蛋白质的预组装复合物引入。
8.根据前述权利要求中任一项所述的方法，其中所述至少一种碱基编辑器复合物或所述至少一种STEME复合物通过生物或物理手段引入细胞系统中，包括转染，转化，包括通过农杆菌属优选根癌农杆菌的转化，病毒载体，基因枪轰击，使用化学试剂的转染，包括聚乙二醇转染，或其任何组合。
9.根据前述权利要求中任一项的方法，其中所述至少一个感兴趣的核酸序列是与农艺学上重要的表型相关的内源基因或遗传元件。
10.根据权利要求9所述的方法，其中所述内源基因选自编码对非生物胁迫的抗性或耐受性的基因，所述非生物胁迫包括干旱胁迫、渗透胁迫、热胁迫、冷胁迫、氧化胁迫、重金属胁迫、氮缺乏、磷酸盐缺乏、盐胁迫或水涝，编码除草剂抗性，包括对草甘膦、草铵膦/膦丝菌素、潮霉素、原卟啉原氧化酶(PPO)抑制剂、ALS抑制剂和麦草畏的抗性的基因，编码对生物胁迫抗性或耐受性的基因，包括病毒抗性基因、真菌抗性基因、细菌抗性基因、昆虫抗性基因，或编码产量相关性状的基因，包括抗倒伏性、开花时间、抗落粒性、种子颜色、胚乳组成，或营养含量。
11.根据权利要求9所述的方法，其中所述遗传元件是调控序列的至少一部分，其中所述调控序列包括以下至少一种：核心启动子序列、近端启动子序列、顺式调控序列中、反式调控序列、基因座控制序列、绝缘子序列、沉默子序列、增强子序列、终止子序列和/或它们的任何组合。
12.根据前述权利要求中任一项所述的方法，其中所述至少一种碱基编辑器复合物诱导感兴趣的核酸序列中至少1、至少2、至少3、至少4、至少5、至少6、至少7、至少8、至少9、至少10、至少11、至少12、至少13、至少14、至少15、至少16、至少17、至少18、至少19、20、至少21、至少22、至少23、至少24、至少25或甚至更多个的核苷酸交换。
13.根据前述权利要求中任一项所述的方法，其中所述至少一种碱基编辑器复合物的至少一种碱基编辑器组分包含至少一个核酸识别结构域和至少一个核酸编辑结构域以及所述至少一种STEME复合物的至少一个STEME组分包含至少一个核酸识别结构域和至少两个核酸编辑结构域，其中至少一个核酸识别结构域独立地选自任何CRISPR核酸酶的解除武装和切口酶形式，包括但不限于CRISPR-dCas9、CRISPR-dCpf1、CRISPR-dCsm1、CRISPR-dCasX、CRISPR-dCasY、CRISPR-dMAD7、CRISPR-Cas9切口酶、CRISPR-Cpf1切口酶、CRISPR-Csm1切口酶、CRISPR-CasY切口酶切口酶或CRISPR-MAD7切口酶，并且其中至少一个或至少两个核酸编辑结构域独立地选自胞苷脱氨酶或腺嘌呤脱氨酶，优选其中至少一个核酸编辑结构域独立地选自载脂蛋白BmRNA编辑复合物(APOBEC)家族脱氨酶，优选大鼠衍生的APOBEC、激活诱导的胞苷脱氨酶(AID)、ACF1/ASE脱氨酶、ADAT家族脱氨酶、ADAR2脱氨酶、或PmCDA1脱氨酶、TadA衍生的脱氨酶和/或其任何组合、变体或催化活性片段，并且其中至少一种位点特异性碱基编辑器任选地包含至少一种核定位信号，并且其中在至少一种碱基编辑器任选地包含至少一种接头序列，优选地XTEN接头，并且其中所述至少一种碱基编辑器任选地包含抑制天然存在的DNA或RNA修复的至少一种成分，优选地尿嘧啶DNA糖基化酶抑制剂(UGI)结构域、噬菌体Mu的Gam蛋白结构域、或肌苷碱基切除修复结构域的抑制剂。
14.根据权利要求13的方法，其中至少一种碱基编辑器组件或编码其的序列，或至少一种STEME组件或编码其的序列，以融合分子的形式提供。
15.根据权利要求13的方法，其中碱基编辑器复合物的组分或编码其的序列，或STEME复合物的组分或编码其的序列，作为单独的分子提供。
16.根据前述权利要求中任一项的方法，其中所述细胞系统选自真核生物，其中所述真核生物是植物、植物的一部分或植物细胞。
17.根据权利要求16的方法，其中所述植物的部分选自叶、茎、根、出芽的胚根、花、花部分、花瓣、果实、花粉、花粉管、花药丝、胚珠、胚囊、卵细胞、子房、合子、胚、合子胚、体细胞胚胎、顶端分生组织、维管束、周轮、种子、根和插条。
18.根据权利要求16或17所述的方法，其中所述植物、植物的一部分或植物细胞是或源自选自以下的植物物种：大麦(Hordeum vulgare)、球茎大麦(Hordeum bulbusom)、双色高粱(Sorghum bicolor)、甘蔗(Saccharum officinarium)、玉米(Zea mays)、小米(Setariaitalic)、小粒稻(Oryza minuta)、水稻(Oryza sativa)、澳洲野生稻(Oryzaaustraliensis)、高秆野生稻(Oryza alta)、普通小麦(Triticum aestivum)、黑麦(Secalecereale)、苹果(Malus domestica)、紫短柄草(Brachypodium distich-yon)、海滨大麦(Hordeum marinum)、节节麦(Aegilops tauschii)、Daucus glochidiatus、甜菜(Betavulgaris)、小胡萝卜(Daucus pusillus)、Daucus muricatus、胡萝卜(Daucus carota)、巨桉(Eucalyptus grandis)、美花烟草(Nicotiana sylvestris)、绒毛状烟草(Nicotianatomentosiformis)、烟草(Nicotiana tabacum)、番茄(Solanum lycopersicum)、马铃薯(Solanum tuberosum)、中果咖啡(Coffea canephora)、葡萄(Vitis vinifera)、Erythrante guttata、螺旋狸藻(Genlisea aurea)、黄瓜(Cucumis sativus)、川桑(Morusnotabilis)、Arabidopsis arenosa、深山南芥(Arabidopsis lyrata)、拟南芥(Arabidopsis thaliana)、喜马拉雅鼠耳芥(Crucihimalaya himalaica)、卵叶须弥芥(Crucihimalaya wallichii)、弯曲碎米荠(Cardamine flexuosa)、北美独行菜(Lepidiumvirginicum)、荠菜(Capsella bursa pastoris)、Olmarabidopsis pumila、筷子芥(Arabishirsute)、欧洲油菜(Brassica napus)、甘蓝(Brassica oeleracia)、芜菁(Brassicarapa)、萝卜(Raphanus sativus)、芥菜(Brassica juncea)、黑芥(Brassica nigra)、Erucavesicaria subsp.sativa、甜橙(Citrus sinensis)、麻风树(Jatropha curcas)、毛果杨(Populus trichocarpa)、蒺藜状苜蓿(Medicago truncatula)、山下鹰嘴豆(Cicer yama-shitae)、Cicer bijugum、鹰嘴豆(Cicer arietinum)、网状鹰嘴豆(Cicer reticulatum)、Cicer judaicum、木豆(Cajanus cajanifolius)、蔓草虫豆(Cajanus scarabaeoides)、菜豆(Phaseolus vulgaris)、大豆(Glycine max)、棉属(Gossypium sp.)、紫云英(Astragalus sinicus)、百脉根(Lotus japonicas)、夏堇(Torenia fournieri)、Spinaceaoleracea、菜豆(Phaseolus vulgaris)、蚕豆(Vicia faba)、洋葱(Allium cepa)、葱(Allium fistulosum)、蒜(Allium sativum)、和韭菜(Allium tuberosum)。
19.一种通过根据权利要求3-18中任一项的方法获得的修饰的细胞系统。
20.包含向导RNA阵列的至少一种碱基编辑器复合物或至少一种STEME复合物的用途，所述向导RNA靶向细胞系统的遗传物质中的至少一种感兴趣的核酸序列，所述用途是用于
(a)产生具有农艺学重要表型的细胞系统，该表型与至少一种感兴趣的核酸序列中的至少一种修饰相关；和/或
(b)鉴定与细胞系统遗传物质中至少一种感兴趣的核酸序列中的至少一种修饰相关的农艺学重要表型。
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