BR112021008427A8 - Mutagênese direcionada usando editores de bases - Google Patents

Mutagênese direcionada usando editores de bases

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Publication number
BR112021008427A8
BR112021008427A8 BR112021008427A BR112021008427A BR112021008427A8 BR 112021008427 A8 BR112021008427 A8 BR 112021008427A8 BR 112021008427 A BR112021008427 A BR 112021008427A BR 112021008427 A BR112021008427 A BR 112021008427A BR 112021008427 A8 BR112021008427 A8 BR 112021008427A8
Authority
BR
Brazil
Prior art keywords
targeted mutagenesis
complex
present
steme
agronomically
Prior art date
Application number
BR112021008427A
Other languages
English (en)
Other versions
BR112021008427A2 (pt
Inventor
Mei Yu
Hummel Aaron
Vaghchhipawala Zarir
Gao Caixia
Li Chao
Zhang Rui
Original Assignee
Kws Saat Se & Co Kgaa
Inst Genetics & Developmental Biology Cas
Shanghai Bluecross Medical Science Inst
Suzhou Qi Biodesign Biotechnology Company Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Kws Saat Se & Co Kgaa, Inst Genetics & Developmental Biology Cas, Shanghai Bluecross Medical Science Inst, Suzhou Qi Biodesign Biotechnology Company Ltd filed Critical Kws Saat Se & Co Kgaa
Publication of BR112021008427A2 publication Critical patent/BR112021008427A2/pt
Publication of BR112021008427A8 publication Critical patent/BR112021008427A8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46—Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4636—Oryza sp. [rice]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1058—Directional evolution of libraries, e.g. evolution of libraries is achieved by mutagenesis and screening or selection of mixed population of organisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes
    • C12N2320/13—Applications; Uses in screening processes in a process of directed evolution, e.g. SELEX, acquiring a new function
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Ecology (AREA)
  • Cell Biology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Physiology (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

MUTAGÊNESE DIRECIONADA USANDO EDITORES DE BASES. A presente invenção refere-se a métodos para descobrir traços e gerar sistemas celulares com fenótipos aprimorados. Em particular, a presente invenção fornece métodos para o desenvolvimento de plantas com fenótipos agronomicamente otimizados usando mutagênese direcionada com poucos ou nenhum efeito fora-do-alvo. Mutagênese direcionada é alcançada pela introdução de um complexo editor de bases ou de um complexo STEME que compreende uma série de RNAs guia que têm como alvo uma sequência de ácidos nucleicos de interesse. A presente invenção refere-se também a sistemas celulares obtidos por meio dos métodos descritos no presente documento e ao uso de um complexo editor de bases ou o complexo STEME que compreende uma matriz de RNAs guia para gerar um sistema celular que tem um fenótipo agronomicamente importante e para a identificação de um fenótipo agronomicamente importante.
BR112021008427A 2018-11-01 2019-11-04 Mutagênese direcionada usando editores de bases BR112021008427A8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862754281P 2018-11-01 2018-11-01
US62/754,281 2018-11-01
PCT/EP2019/080140 WO2020089489A1 (en) 2018-11-01 2019-11-04 Targeted mutagenesis using base editors

Publications (2)

Publication Number Publication Date
BR112021008427A2 BR112021008427A2 (pt) 2021-09-28
BR112021008427A8 true BR112021008427A8 (pt) 2023-05-09

Family

ID=68470510

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112021008427A BR112021008427A8 (pt) 2018-11-01 2019-11-04 Mutagênese direcionada usando editores de bases

Country Status (6)

Country Link
US (1) US20210403901A1 (pt)
EP (1) EP3956443A1 (pt)
CN (1) CN113874501A (pt)
BR (1) BR112021008427A8 (pt)
CA (1) CA3118340A1 (pt)
WO (1) WO2020089489A1 (pt)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2021212189A1 (en) * 2020-01-30 2022-08-04 Pairwise Plants Services, Inc. Compositions, systems, and methods for base diversification
CN111549053B (zh) * 2020-05-22 2022-06-07 北京市农林科学院 一种花椰菜单核苷酸突变的方法
CN113215190B (zh) * 2021-06-03 2022-09-27 西南大学 CRISPR/Cas9介导的基因编辑技术制备柑橘纯合突变体的方法
WO2023050158A1 (zh) * 2021-09-29 2023-04-06 深圳先进技术研究院 一种实现多碱基编辑的方法
CN114395585B (zh) * 2022-01-12 2024-03-08 中国科学院天津工业生物技术研究所 用于碱基编辑的组合物
IL292199B2 (en) * 2022-04-12 2024-02-01 Plantarc Bio Ltd A method for optimizing gene expression levels in plants
WO2023232024A1 (en) * 2022-05-30 2023-12-07 Wuhan University System and methods for duplicating target fragments
WO2023247753A1 (en) * 2022-06-23 2023-12-28 BASF Agricultural Solutions Seed US LLC Diversifying base editing
CN115820691B (zh) * 2022-07-25 2023-08-22 安徽农业大学 一种基于LbCpf1变体的水稻碱基编辑系统和应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20150166982A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting pi3k point mutations
EP3613854A1 (en) 2014-03-05 2020-02-26 National University Corporation Kobe University Genomic sequence modification method for specifically converting nucleic acid bases of targeted dna sequence, and molecular complex for use in same
BR112017003555A2 (pt) * 2014-08-28 2017-12-12 Kws Saat Se & Co Kgaa geração de plantas haploides
EP3095870A1 (en) * 2015-05-19 2016-11-23 Kws Saat Se Methods for the in planta transformation of plants and manufacturing processes and products based and obtainable therefrom
IL294014B1 (en) 2015-10-23 2024-03-01 Harvard College Nucleobase editors and their uses
AU2017306676B2 (en) 2016-08-03 2024-02-22 President And Fellows Of Harvard College Adenosine nucleobase editors and uses thereof
EP3582609A1 (en) * 2017-02-15 2019-12-25 Keygene N.V. Methods of targeted genetic alteration in plant cells

Also Published As

Publication number Publication date
US20210403901A1 (en) 2021-12-30
CN113874501A (zh) 2021-12-31
CA3118340A1 (en) 2020-05-07
BR112021008427A2 (pt) 2021-09-28
WO2020089489A1 (en) 2020-05-07
EP3956443A1 (en) 2022-02-23

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Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: KWS SAAT SE AND CO. KGAA (DE) ; SHANGHAI BLUECROSS MEDICAL SCIENCE INSTITUTE (CN)

B25A Requested transfer of rights approved

Owner name: KWS SAAT SE AND CO. KGAA (DE) ; SUZHOU QI BIODESIGN BIOTECHNOLOGY COMPANY LIMITED (CN)