MX2020013778A - Composiciones, sistemas y metodos de amplificacion basados en crispr/cas y transposasa. - Google Patents

Composiciones, sistemas y metodos de amplificacion basados en crispr/cas y transposasa.

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Publication number
MX2020013778A
MX2020013778A MX2020013778A MX2020013778A MX2020013778A MX 2020013778 A MX2020013778 A MX 2020013778A MX 2020013778 A MX2020013778 A MX 2020013778A MX 2020013778 A MX2020013778 A MX 2020013778A MX 2020013778 A MX2020013778 A MX 2020013778A
Authority
MX
Mexico
Prior art keywords
target nucleic
nucleic acid
systems
methods
crispr
Prior art date
Application number
MX2020013778A
Other languages
English (en)
Inventor
Feng Zhang
Max Kellner
Jonathan Gootenberg
Omar Abudayyeh
Original Assignee
Broad Inst Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Broad Inst Inc filed Critical Broad Inst Inc
Publication of MX2020013778A publication Critical patent/MX2020013778A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/101—Temperature
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/125—Specific component of sample, medium or buffer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/13—Plant traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

En el presente documento se proporcionan métodos y sistemas para detectar una secuencia de ácido nucleico diana. El método comprende poner en contacto un oligonucleótido que comprende la secuencia de ácido nucleico diana con un complejo de transposón; insertar uno o más promotores de T7 ARN en el oligonucleótido utilizando la transposasa; y (c) amplificar la secuencia de ácido nucleico diana. El complejo de transposón puede comprender una transposasa y una secuencia de transposón que comprende uno o más promotores de T7 ARN. La secuencia nucleica diana se puede amplificar generando oligonucleótidos de ARN que comprenden la secuencia de ácido nucleico diana mediante la transcripción de uno o más promotores de T7 ARN insertados. El ácido nucleico diana amplificado puede detectarse utilizando un sistema de detección basado en CRISPR Cas13.
MX2020013778A 2018-06-26 2019-06-26 Composiciones, sistemas y metodos de amplificacion basados en crispr/cas y transposasa. MX2020013778A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201862690160P 2018-06-26 2018-06-26
US201862767077P 2018-11-14 2018-11-14
PCT/US2019/039195 WO2020006049A1 (en) 2018-06-26 2019-06-26 Crispr/cas and transposase based amplification compositions, systems and methods

Publications (1)

Publication Number Publication Date
MX2020013778A true MX2020013778A (es) 2021-05-27

Family

ID=67263112

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2020013778A MX2020013778A (es) 2018-06-26 2019-06-26 Composiciones, sistemas y metodos de amplificacion basados en crispr/cas y transposasa.

Country Status (11)

Country Link
US (1) US20210147915A1 (es)
EP (1) EP3814519A1 (es)
JP (1) JP2021528975A (es)
KR (1) KR20210024009A (es)
CN (1) CN112368396A (es)
AU (1) AU2019294690A1 (es)
BR (1) BR112020025319A2 (es)
CA (1) CA3101066A1 (es)
MX (1) MX2020013778A (es)
SG (1) SG11202011240YA (es)
WO (1) WO2020006049A1 (es)

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WO2023004391A2 (en) * 2021-07-21 2023-01-26 Montana State University Nucleic acid detection using type iii crispr complex
KR102576803B1 (ko) * 2022-10-14 2023-09-12 주식회사 풀무원 CRISPR-Cas 기반의 리스테리아 모노사이토제네스 검출용 조성물 및 이를 이용한 리스테리아 모노사이토제네스 검출방법

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Also Published As

Publication number Publication date
WO2020006049A1 (en) 2020-01-02
KR20210024009A (ko) 2021-03-04
BR112020025319A2 (pt) 2021-03-09
SG11202011240YA (en) 2020-12-30
CA3101066A1 (en) 2020-01-02
JP2021528975A (ja) 2021-10-28
US20210147915A1 (en) 2021-05-20
AU2019294690A1 (en) 2020-12-03
CN112368396A (zh) 2021-02-12
EP3814519A1 (en) 2021-05-05

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