MX2021007302A - Metodos y composiciones para la deteccion de productos de amplificacion. - Google Patents

Metodos y composiciones para la deteccion de productos de amplificacion.

Info

Publication number
MX2021007302A
MX2021007302A MX2021007302A MX2021007302A MX2021007302A MX 2021007302 A MX2021007302 A MX 2021007302A MX 2021007302 A MX2021007302 A MX 2021007302A MX 2021007302 A MX2021007302 A MX 2021007302A MX 2021007302 A MX2021007302 A MX 2021007302A
Authority
MX
Mexico
Prior art keywords
detection
compositions
methods
amplification products
droplet
Prior art date
Application number
MX2021007302A
Other languages
English (en)
Inventor
Rixun Fang
Yuh- Min CHIANG
Ronald Phillip Chiarello
Original Assignee
Alveo Tech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Alveo Tech Inc filed Critical Alveo Tech Inc
Publication of MX2021007302A publication Critical patent/MX2021007302A/es

Links

Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/16—Reagents, handling or storing thereof
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0893—Geometry, shape and general structure having a very large number of wells, microfabricated wells
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/18—Means for temperature control
    • B01L2300/1805—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks
    • B01L2300/1811—Conductive heating, heat from thermostatted solids is conducted to receptacles, e.g. heating plates, blocks using electromagnetic induction heating

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Organic Chemistry (AREA)
  • Dispersion Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Clinical Laboratory Science (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Algunas modalidades de los sistemas, dispositivos, kits y métodos proporcionados en la presente se refieren a la amplificación y detección de un ácido nucleico diana. Algunas de estas modalidades incluyen una gota que comprende una mezcla de reacción acuosa y un aceite, y una unidad de detección. Algunas modalidades incluyen un pasaje o conducto configurado para transportar la gota. En algunas modalidades, la unidad de detección incluye una unidad generadora de campo eléctrico y un elemento de electro-detección.
MX2021007302A 2018-12-20 2019-12-18 Metodos y composiciones para la deteccion de productos de amplificacion. MX2021007302A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862783051P 2018-12-20 2018-12-20
PCT/US2019/067082 WO2020132010A1 (en) 2018-12-20 2019-12-18 Methods and compositions for detection of amplification products

Publications (1)

Publication Number Publication Date
MX2021007302A true MX2021007302A (es) 2021-09-08

Family

ID=71102309

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021007302A MX2021007302A (es) 2018-12-20 2019-12-18 Metodos y composiciones para la deteccion de productos de amplificacion.

Country Status (8)

Country Link
US (1) US20220048031A1 (es)
EP (1) EP3899025A4 (es)
JP (1) JP2022516442A (es)
CN (1) CN113423812A (es)
AU (1) AU2019405733A1 (es)
CA (1) CA3123832A1 (es)
MX (1) MX2021007302A (es)
WO (1) WO2020132010A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20210121884A1 (en) * 2012-12-21 2021-04-29 Leica Biosystems Melbourne Pty Ltd Method of producing a reagent on-board an instrument
US10196678B2 (en) 2014-10-06 2019-02-05 ALVEO Technologies Inc. System and method for detection of nucleic acids
MX2019003273A (es) 2016-09-23 2019-09-13 Alveo Tech Inc Metodos y composiciones para detectar analitos.
KR102486062B1 (ko) * 2021-04-05 2023-01-06 인제대학교 산학협력단 액적의 크기 제어가 가능한 능동형 액적 생성장치, 이를 이용한 액적 크기 제어방법 및 액적 생성 자가진단 장치
WO2023141532A2 (en) * 2022-01-21 2023-07-27 The Board Of Trustees Of The Leland Stanford Junior University Detection of condensed amplification products
CN114807403A (zh) * 2022-06-24 2022-07-29 中国医学科学院北京协和医院 一种用于检测普罗威登斯菌的核酸试剂和数字pcr试剂盒
CN115287176A (zh) * 2022-09-30 2022-11-04 潍坊安普未来生物科技有限公司 一种两步法等温核酸扩增检测微流控装置

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5645801A (en) * 1993-10-21 1997-07-08 Abbott Laboratories Device and method for amplifying and detecting target nucleic acids
US7439014B2 (en) * 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US9562837B2 (en) * 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
WO2007133710A2 (en) * 2006-05-11 2007-11-22 Raindance Technologies, Inc. Microfluidic devices and methods of use thereof
WO2010005593A1 (en) * 2008-07-11 2010-01-14 President And Fellows Of Harvard College Systems and methods of droplet-based selection
US9376713B2 (en) * 2009-09-23 2016-06-28 The Board Of Trustees Of The University Of Illinois Label free detection of nucleic acid amplification
WO2012142192A2 (en) * 2011-04-11 2012-10-18 Gray Mark A Apparatus and method for making uniform emulsions
WO2013165748A1 (en) * 2012-04-30 2013-11-07 Raindance Technologies, Inc Digital analyte analysis
WO2015175843A1 (en) * 2014-05-14 2015-11-19 The Regents Of The University Of California Interfacial concentration and orientation of droplet contents for enhanced detection using electrical impedance spectroscopy
MX2019003273A (es) * 2016-09-23 2019-09-13 Alveo Tech Inc Metodos y composiciones para detectar analitos.

Also Published As

Publication number Publication date
CA3123832A1 (en) 2020-06-25
JP2022516442A (ja) 2022-02-28
CN113423812A (zh) 2021-09-21
WO2020132010A1 (en) 2020-06-25
AU2019405733A1 (en) 2021-07-15
EP3899025A1 (en) 2021-10-27
US20220048031A1 (en) 2022-02-17
EP3899025A4 (en) 2023-02-22

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