PH12018502286A1 - Rt-pcr-hrm or pcr-hrm primer, reagent and method for rapidly distinguishing four serotypes of dengue virus - Google Patents

Rt-pcr-hrm or pcr-hrm primer, reagent and method for rapidly distinguishing four serotypes of dengue virus

Info

Publication number
PH12018502286A1
PH12018502286A1 PH12018502286A PH12018502286A PH12018502286A1 PH 12018502286 A1 PH12018502286 A1 PH 12018502286A1 PH 12018502286 A PH12018502286 A PH 12018502286A PH 12018502286 A PH12018502286 A PH 12018502286A PH 12018502286 A1 PH12018502286 A1 PH 12018502286A1
Authority
PH
Philippines
Prior art keywords
pcr
hrm
reagent
dengue virus
serotypes
Prior art date
Application number
PH12018502286A
Inventor
Bingfeng Yan
Pengju Guo
Ren Huang
Fuchun Zhang
Lingzhai Zhao
Original Assignee
Guangdong Lab Animals Monitoring Inst
Guangzhou Eighth Peoples Hospital
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Guangdong Lab Animals Monitoring Inst, Guangzhou Eighth Peoples Hospital filed Critical Guangdong Lab Animals Monitoring Inst
Publication of PH12018502286A1 publication Critical patent/PH12018502286A1/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

The present invention discloses an RT-PCR-HRM or PCR-HRM primer, reagent, and method for rapidly distinguishing four types of serum dengue virus.
PH12018502286A 2016-04-27 2018-10-26 Rt-pcr-hrm or pcr-hrm primer, reagent and method for rapidly distinguishing four serotypes of dengue virus PH12018502286A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/CN2016/080299 WO2017185244A1 (en) 2016-04-27 2016-04-27 Rt-pcr-hrm or pcr-hrm primer, reagent, and method for rapidly distinguishing four types of serum dengue virus

Publications (1)

Publication Number Publication Date
PH12018502286A1 true PH12018502286A1 (en) 2019-07-15

Family

ID=60160625

Family Applications (1)

Application Number Title Priority Date Filing Date
PH12018502286A PH12018502286A1 (en) 2016-04-27 2018-10-26 Rt-pcr-hrm or pcr-hrm primer, reagent and method for rapidly distinguishing four serotypes of dengue virus

Country Status (2)

Country Link
PH (1) PH12018502286A1 (en)
WO (1) WO2017185244A1 (en)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110760614B (en) * 2019-04-29 2023-06-20 杭州市上城区疾病预防控制中心 Method for quickly detecting, parting and tracing dengue virus nucleic acid
CN110982940A (en) * 2019-12-30 2020-04-10 广州市疾病预防控制中心 Composition and kit for detecting nucleic acid of measles virus, rubella virus and mumps virus based on melting curve
WO2021173597A1 (en) * 2020-02-28 2021-09-02 Merck Sharp & Dohme Corp. Dengue serotype specific rt-pcr multiplex assay
CN113430304A (en) * 2021-08-16 2021-09-24 广州医科大学附属市八医院 Multiplex real-time fluorescent quantitative PCR primer, probe, kit and use method of kit for dengue virus typing detection

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SG11201502618PA (en) * 2012-10-04 2015-05-28 Univ Leland Stanford Junior Methods and reagents for detection, quantitation, and serotyping of dengue viruses
CN103173568B (en) * 2013-03-28 2014-12-10 中山大学 Primer group, kit and method for rapidly identifying 1 type dengue fever virus
CN104328222B (en) * 2014-11-17 2015-11-25 扬州大学 The test kit of reverse transcription PCR detection and somatotype dengue virus and detection method thereof
CN104711373A (en) * 2015-04-03 2015-06-17 张瑾 Method for preparing RNase-resistant dengue virus nucleic acid detection quality control product

Also Published As

Publication number Publication date
WO2017185244A1 (en) 2017-11-02

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