WO2017171092A8 - 植物に物質を導入する方法 - Google Patents

植物に物質を導入する方法 Download PDF

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Publication number
WO2017171092A8
WO2017171092A8 PCT/JP2017/013868 JP2017013868W WO2017171092A8 WO 2017171092 A8 WO2017171092 A8 WO 2017171092A8 JP 2017013868 W JP2017013868 W JP 2017013868W WO 2017171092 A8 WO2017171092 A8 WO 2017171092A8
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WIPO (PCT)
Prior art keywords
enzyme
egg cell
plant
fertilized egg
cell
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PCT/JP2017/013868
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English (en)
French (fr)
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WO2017171092A1 (ja
Inventor
加藤 紀夫
龍史 岡本
隆敏 木羽
絵梨香 戸田
Original Assignee
日本たばこ産業株式会社
公立大学法人首都大学東京
国立研究開発法人 理化学研究所
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Application filed by 日本たばこ産業株式会社, 公立大学法人首都大学東京, 国立研究開発法人 理化学研究所 filed Critical 日本たばこ産業株式会社
Priority to EP17775623.6A priority Critical patent/EP3437464A4/en
Priority to CN201780018863.3A priority patent/CN109310061B/zh
Priority to CA3018824A priority patent/CA3018824A1/en
Priority to US16/089,813 priority patent/US11549120B2/en
Priority to BR112018069826A priority patent/BR112018069826A2/pt
Priority to AU2017245117A priority patent/AU2017245117A1/en
Priority to RU2018138200A priority patent/RU2018138200A/ru
Publication of WO2017171092A1 publication Critical patent/WO2017171092A1/ja
Publication of WO2017171092A8 publication Critical patent/WO2017171092A8/ja

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8206Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by physical or chemical, i.e. non-biological, means, e.g. electroporation, PEG mediated
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • A01H3/04Processes for modifying phenotypes, e.g. symbiosis with bacteria by treatment with chemicals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H6/00Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
    • A01H6/46Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize
    • A01H6/4684Zea mays [maize]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01015Polygalacturonase (3.2.1.15)

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

本発明は、植物に物質を導入する方法に関する。本発明の方法は、以下の工程:(1-i)植物の受精卵細胞を含む組織から受精卵細胞を単離し、その後、当該受精卵細胞を植物組織分解酵素を含む酵素溶液で低力価条件処理する、(1-ii)植物の受精卵細胞を含む組織を、植物組織分解酵素を含む酵素溶液で低力価条件処理し、次いで、酵素処理された受精卵細胞を単離する、(1-iii)植物の受精卵細胞を含む組織を、植物組織分解酵素を含む酵素溶液で低力価条件処理すると同時に、酵素処理された受精卵細胞を単離する、(1-iv)植物体から卵細胞および精細胞を単離し、それらを融合することで受精卵を作出し、その後、当該受精卵細胞を、植物組織分解酵素を含む酵素溶液で低力価条件処理する、あるいは、(1-v)植物の卵細胞を含む組織を、植物組織分解酵素を含む酵素溶液で低力価条件処理し、次いで、酵素処理された卵細胞を単離、さらに、単離した精細胞と融合させる、ことにより、酵素処理された単離受精卵細胞を取得し;そして、(2)得られた酵素処理された単離受精卵細胞に、核酸、タンパク質及びペプチドからなる群から選択される物質を導入する、ことを含む。
PCT/JP2017/013868 2016-03-31 2017-03-28 植物に物質を導入する方法 WO2017171092A1 (ja)

Priority Applications (7)

Application Number Priority Date Filing Date Title
EP17775623.6A EP3437464A4 (en) 2016-03-31 2017-03-28 METHOD FOR INDUCING SUBSTANCE IN A PLANT
CN201780018863.3A CN109310061B (zh) 2016-03-31 2017-03-28 将物质导入植物的方法
CA3018824A CA3018824A1 (en) 2016-03-31 2017-03-28 Method for introducing substance into plant
US16/089,813 US11549120B2 (en) 2016-03-31 2017-03-28 Method for introducing substance into plant
BR112018069826A BR112018069826A2 (pt) 2016-03-31 2017-03-28 método de introdução de substância em plantas
AU2017245117A AU2017245117A1 (en) 2016-03-31 2017-03-28 Method for introducing substance into plant
RU2018138200A RU2018138200A (ru) 2016-03-31 2017-03-28 Способ введения вещества в растение

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2016-070288 2016-03-31
JP2016070288A JP2019129705A (ja) 2016-03-31 2016-03-31 植物に物質を導入する方法

Publications (2)

Publication Number Publication Date
WO2017171092A1 WO2017171092A1 (ja) 2017-10-05
WO2017171092A8 true WO2017171092A8 (ja) 2018-08-23

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PCT/JP2017/013868 WO2017171092A1 (ja) 2016-03-31 2017-03-28 植物に物質を導入する方法

Country Status (9)

Country Link
US (1) US11549120B2 (ja)
EP (1) EP3437464A4 (ja)
JP (1) JP2019129705A (ja)
CN (1) CN109310061B (ja)
AU (1) AU2017245117A1 (ja)
BR (1) BR112018069826A2 (ja)
CA (1) CA3018824A1 (ja)
RU (1) RU2018138200A (ja)
WO (1) WO2017171092A1 (ja)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2020072645A (ja) * 2017-01-31 2020-05-14 日本たばこ産業株式会社 植物に物質を導入する方法
WO2019131426A1 (ja) * 2017-12-26 2019-07-04 国立大学法人徳島大学 電気穿孔法による植物組織への直接核酸導入法およびその成果物
JP2022078383A (ja) * 2019-03-29 2022-05-25 日本たばこ産業株式会社 単離された植物細胞の凝集を抑制する方法
CN111139257B (zh) * 2020-01-15 2022-10-14 南京农业大学 一种梨pmei蛋白体外表达的方法及其应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61265022A (ja) * 1985-05-21 1986-11-22 三井東圧化学株式会社 イネプロトプラストからの植物体の再生方法
FI864720A (fi) * 1985-11-22 1987-05-23 Ciba Geigy Ag Direkt gentransmission i plasticider och mitokondrier.
JPH0671398B2 (ja) * 1986-04-02 1994-09-14 三菱商事株式会社 雑種植物の製造方法
DK94892A (da) * 1992-07-23 1994-01-24 Carlsberg Forskningscenter Fremgangsmåde til at regenerere protoplaster, celler eller cellevæv fra planter til hele planter, medium til brug for regenerering, fremgangsmåder til at isolere ægprotoplaster og synergideprotoplaster fra frøanlæg, de ved fremgangsmåderne isolerede ægprotoplaster og synergideprotoplaster samt fremgangsmåde til transformering af befrugtede ægcelle
KR20060061354A (ko) * 2003-08-13 2006-06-07 니뽄 다바코 산교 가부시키가이샤 식물재료로의 유전자 도입을 행하는 방법
EP1652930A1 (en) 2004-10-25 2006-05-03 De Ruiter Seeds R&D B.V. Botrytis-resistant tomato plants
CN101358181A (zh) * 2008-09-09 2009-02-04 南京大学 一种水稻根系木质部薄壁细胞原生质体的分离方法
EP3260544A1 (en) 2008-11-18 2017-12-27 Commonwealth Scientific and Industrial Research Organisation Enzymes and methods for producing omega-3 fatty acids
PT3354657T (pt) * 2009-09-22 2022-05-06 Medicago Inc Método de preparação de proteínas derivadas de plantas
CN101792731B (zh) * 2010-01-20 2012-01-25 河南科技大学 一种分离毛白杨胚珠及珠心的方法
CN102373235B (zh) * 2011-10-17 2013-03-27 南京林业大学 一种将外源基因转入杨树原生质体进行瞬时表达的方法
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Also Published As

Publication number Publication date
JP2019129705A (ja) 2019-08-08
EP3437464A1 (en) 2019-02-06
US20200123553A1 (en) 2020-04-23
CA3018824A1 (en) 2017-10-05
AU2017245117A2 (en) 2018-11-22
US11549120B2 (en) 2023-01-10
CN109310061B (zh) 2022-10-04
BR112018069826A2 (pt) 2019-01-29
CN109310061A (zh) 2019-02-05
WO2017171092A1 (ja) 2017-10-05
RU2018138200A (ru) 2020-04-30
EP3437464A4 (en) 2019-10-16
AU2017245117A1 (en) 2018-11-22
RU2018138200A3 (ja) 2020-07-06

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