KR20150093721A - 원핵생물 경로를 모방함에 의한 식물 자기 질소 고정 - Google Patents

Abstract

식물 색소체 박테리아 nif(질소 고정) 유전자에 대한 표적화 또는 유전자에서의 발현에 의해서 시아노박테리아와 같은 광합성 박테리아, 또는 다른 박테리아의 질소 고정 메커니즘을 복제하는 트랜스제닉 식물을 조작하는 방법이 제공된다. 광합성 박테리아의 질소 고정 메커니즘을 복제하는 식물을 사용하여 토양에서 질소의 전체 농도를 감소시키는 방법이 제공된다. 광합성 박테리아의 질소 고정 메커니즘을 복제하는 식물의 자손이 또한 제공된다.

Description

원핵생물 경로를 모방함에 의한 식물 자기 질소 고정{PLANT SELF NITROGEN FIXATION BY MIMICKING PROKARYOTIC PATHWAYS}

관련 출원의 상호 참조

본 출원은 2013년 7월 25일자 제출된 미국 임시 출원 제61/858,218호와 2012년 12월 3일자 제출된 미국 임시 출원 제61/732,490호에 대한 우선권을 주장하며, 이들은 각각 그 전체가 참고자료로 여기 포함된다.

기술분야

본 개시는 박테리아 nif(질소 고정) 유전자를 표적화하거나 발현함으로써 효소 니트로게나아제의 구조 단백질을 생성하고, 니트로게나아제의 구조 단백질에 단백질 상동체를 코딩하는 합성 DNA 서열을 도입함에 의한, 예를 들어 시아노박테리아와 같은 광합성 박테리아 또는 다른 박테리아의 질소 고정 메커니즘을 복제하는 신규 트랜스제닉 식물, 및 이러한 식물의 제조 방법에 관한 것이다.

식물은 식물계에 속하는 살아있는 유기체이다. 모든 고등 식물은 맥관 조직인 물관부(주로 물과 광물질 수송)와 체관부(주로 당과 다른 대사물질 수송)를 가진다. 유관속 식물은 씨를 가진 모든 식물(겉씨식물 및 속씨식물)과 양치식물(양치류, 석송류 및 속새류)을 포함하며, 이들은 또한 관다발식물이라고 한다. 이들은 광합성 동안 탄소 고정을 위한 에너지원으로서 햇빛과 같은 빛을 사용할 수 있는 능력을 가진 엽록체(또는 다른 종류의 색소체)와 핵(대부분 세포에)을 포함하는 조직화된 세포 구조를 가진 진핵생물이다. 다른 종류의 식물은, 예를 들어 조류, 이끼 및 진균을 포함한다.

농작물의 작물 생산성에 대한 일반적인 제한 요인은 토양과 물의 질소 함량이다. 농업 생산이 인구 증가에 따른 증가된 요구와 감소된 농지 이용률에 보조를 맞추려고 시도함에 따라서 이 원소의 공급은 경시적으로 감소되었다. 질소는 식물을 포함한 모든 형태의 삶에 필수적인 주요 영양소 중 하나이다. 그러나, 질소는 우선 식물이 이용할 수 있는 형태로 전환되어야 한다. 생물학적 질소 고정(BNF)의 현상은 니트로게나아제 효소를 사용하여 대기의 질소(N₂)를 암모니아(NH₃)로 전환하는 현상이다. BNF는 보통 N₂+8H⁺+8e^-+16ATP>>>2NH₃+H₂+16ADP+16Pi의 화학식으로 표시되거나, 16개의 ATP(아데노신 트리포스페이트 - 세포의 에너지 분자) 분자를 소모하면서 8개의 양성자와 8개의 전자의 존재하에 질소 기체 분자가 2개의 암모니아 분자로 전환된다는 말로 설명된다. 이 반응은 N₂가 삼중결합을 함유하므로 엄청난 양의 에너지를 소모한다. 질소 분자의 결합 에너지는 약 225kcal/mol이다. "니트로게나아제 효소 복합체"를 구성하는 몇몇 박테리아 종들과 식물 간의 공생 관계의 결과로서 자연에서 몇몇 BNF가 수행된다. 니트로게나아제 효소 복합체는 2개의 단백질, 즉 Fe-단백질(Nif-H로 알려진 효소)과 Mo-Fe 단백질(Nif-D-K로 알려진 α 및 β 서브유닛)로 구성된다. 니트로게나아제 복합체는 호모다이머(적어도 2개의 동일한 유닛) Fe(철 보조인자) 단백질과 일시적으로 회합되는 헤테로테트라머(동일한 유닛이 아님) MoFe(철-몰리브데늄 보조인자) 단백질로 이루어진다.

Nif-H 유전자는 철 단백질을 암호화하고, Nif-D와 K 유전자는 몰리브데늄 철 단백질을 암호화한다. 따라서, Nif-D 및 Nif-K 유전자는 이들의 최종 활성 형태에 보조인자로서 Mo 및 Fe를 필요로 한다. Nif-D는 알파 서브유닛이라고도 하는 Nif-D 단백질을 암호화하고, Nif-K는 베타 서브유닛이라고도 하는 Nif-K 단백질을 암호화한다. 다시 말해서, Nif-H는 2개의 동일한 서브유닛을 가진 다이머 효소이고(총 2개 단백질), Nif-D-K는 2α2β 다이머이다(총 4개 단백질). 모두 6개의 서브유닛이 필수적이며, 그것의 기능에 필요하다. 자연에서 Nif-H는 상당히 다양하고, 생물다양성에 기여한다. 여러 종류의 Nif-H가 있다는 사실은 다양한 자연 조건에 대한 적응 능력을 제공한다.

니트로게나아제 효소 복합체를 생성하는 박테리아 종은 시아노박테리아, 아조토박테라세아에, 리조비아 및 프랭키아와 같은 질소자급영양체를 포함한다. 예를 들어, 시아노박테리아에서, Mo-Fe 단백질(Nif-D-K)은 대기의 질소(N₂)와 결합하고, 이어서 Fe-단백질(Nif-H)가 페레독신에 의해서 기증된 전자에 의해 그것을 환원시킨다. 페레독신은 고등 식물 엽록체인 Fe₂S₂ 페레독신과 동일하지는 않지만 유사한 광합성 전자 수송 사슬에서 전자 캐리어로 기능하는 단백질이다. 환원된 Fe-단백질은 Mo-Fe 단백질로 (환원된) 전자를 수송하는데 ATP를 사용할 것이고, Mo-Fe 단백질은 이어서 N₂에 전자를 기증할 것이다(니트로게나아제 리덕타아제). 이 과정을 몇번 반복함으로써 N≡N의 3개의 공유 결합이 모두 2NH₃로 환원된다.

그러나, 아주 소수의 식물 종만이 질소자급영양체와 공생 관계로 살아갈 수 있다. 예를 들어, 콩과의 완두콩 식물은 리조비아 패밀리의 박테리아와 공생관계로 살아간다. 특히, 리조비아 박테리아는 완두콩 식물의 뿌리에 침투하여 뿌리혹을 생성하는데, 이것은 (암모니아로) 질소를 고정하는 박테리아를 함유하고, 식물은 탄소(당)를 기증한다. 따라서, 공생관계의 개선, 혹은 숙주 범위의 확장은 식물 생존에 유익할 것이나, 이 목표를 달성하는 것은 과정의 복잡성 및 기초 지식의 부족을 비롯한 많은 난관을 포함한다.

또한, 질소는 인공적 과정을 사용하여 화학적으로 고정될 수 있다. 질소 고정을 위한 이 방법은 Harber 과정이라고 알려진 열 반응을 통해서 가장 흔히 생성된다. 이 과정은 상대적으로 간단한 반응에 고온과 고압을 필요로 한다. 지난 수백년 동안 질소 비료에 대한 요구는 해마다 200백만 미터 톤을 넘겨 꾸준히 증가했다. 이 소비는 증가할 가능성이 크다.

비-공생 유기체(예를 들어, 질소를 고정하기 위해서 어떤 미생물과도 확립된 공생 관계를 갖지 않는 자유로운 살아있는 유기체)는 이들이 각각 핵심 효소인 니트로게나아제를 포함한다면 자기 생물학적 질소 고정(sBNF)을 달성할 수 있다. 작물 식물과 조류 및 자기-질소 고정이 가능한 다른 식물들처럼 비-박테리아 유기체의 존재는 시비 필요 감소, 시비 오염 감소, 친환경 작물 생산 제공, 증진된 작물 생산, 개선된 식물 중 오일 함량, 개선된 식물 중 단백질 함량, 물의 질소 오염 감소, 및 토양의 유기상과 관련하여 질소 및 탄소에 비해 탄소 함량의 부화와 같은 몇몇 목적을 위해 유용할 것이다. 그러나, 몇몇 이유 때문에 이들 결과를 달성할 수 있다고 알려진 식물은 없는데, 이중 하나는 니트로게나아제라는 특정한 효소 복합체로서, 이것은 질소 고정을 위해 몇몇 단백질 및 유전자를 포함한다.

대기의 질소 고정에 사용되는 거의 모든 핵심 효소는 산소에 매우 민감하다. 이 특징은 식물에서 질소 고정을 거의 불가능하게 하는데, 식물은 광합성 반응 동안 물로부터 산소를 생성하기 때문이다. 시아노박테리아는 엽록체의 진화적 선조(토대)인 것으로 생각된다. 엽록체는 진화 동안 그것의 원래 유전자를 많이 상실했지만, 시아노박테리아는 엽록체가 상실한 유전자를 대부분 유지한다. 시아노박테리아는 광합성 유기체로서 지구상에 빛이 비추는 거의 모든 환경에서 서식한다. 이들은 20개가 넘는 질소 고정(NIF) 유전자들에 의해서 촉진되는 생물학적 질소 고정(BNF) 과정에서 중요한 역할을 한다. 이들 유전자 중 단지 3개만(Nif-D, Nif-K 및 Nif-H)이 NIF 효소(니트로게나아제)이다. NIF 유전자의 나머지는 복잡한 조립, 보조인자 과정, 및 발현 제어에 수반된다.

대두, 콩 또는 완두콩과 같은 콩과의 작물 식물은 공생 박테리아인 리조비움 종(Rhizobium sp.)에 의해서 점유된 뿌리혹을 발생시킬 수 있는 특유의 능력에 대해서 잘 알려져 있다. 이들 박테리아는 BNF가 가능하고 식물에 암모니아를 기증한다. 대부분의 작물 식물은 이 능력을 갖지 않으며, 리조비움 종은 이들과 상호작용하지 못할 것이다. 리지비아의 "숙주" 범위를 확장시키기 위한 몇몇 비공개 시도가 이루어졌지만 모두 실패했다.

따라서, 시스템을 지속가능하며 상업적으로 가치있게 만드는 유의한 속도로 암모니아를 생성하기 위하여 세포성 질소 고정을 할 수 있는 트랜스제닉 식물을 포함하는 시스템 및 방법이 필요하다. 아래 섹션에 이러한 시스템을 설명한다.

본 개시는 자신 스스로 사용가능한 질소원을 생성함으로써 질소원으로서 질소성 비료에 대한 의존성을 감소시키기 위하여 자기/자율 질소 고정 과정을 수행할 수 있도록 형질전환된 트랜스제닉 식물(유전자 변형된 유기체 또는 GMO)에 관한 것이다. 또한, 본 개시는 이들 식물이 자기/자율 질소 고정을 수행할 수 있도록 이들 식물을 형질전환하기 위한 유전자를 포함하는 식물 세포, 조직, 식물 일부 또는 식물 계통에 관한 것이다.

일부 구체예들에서, 본 개시의 원리에 따라서, 하나 이상의 식물 세포를 프로모터 서열 및 터미네이터 서열에 작동가능하게 연결된 Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열과 함께 도입하는 단계; 식물 세포로부터 하나 이상의 식물을 재생시키는 단계 및 하나 이상의 식물이 Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함하도록 증진된 질소 고정을 나타내는 식물 세포로부터 재배된 하나 이상의 식물을 선택하는 단계를 포함하는 방법에 의해서 생성된 변형된 자기/자율 질소 고정 프로파일을 나타내는 식물이 제공된다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열은 단일 유기체로부터 얻어진다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열은 상이한 유기체들로부터 얻어진다. 예를 들어, 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자 중 적어도 하나를 암호화하는 재조합 핵산 서열은 제1 유기체로부터 얻어지고, Nif-H, Nif-D 및 Nif-K 유전자 중 적어도 하나를 암호화하는 재조합 핵산 서열은 제1 종과 상이한 제2 유기체로부터 얻어진다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열은 자연에서 발생한다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열은 합성이며 자연에서 발견되지 않는다.

일부 구체예들에서, 본 개시의 원리에 따라서, 식물 세포를 SEQ. ID. NO. 1, SEQ. ID. NO. 2 및 SEQ. ID. NO. 3 중 하나를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포로부터 하나 이상의 식물을 재생시키는 단계; 및 식물 세포로부터 재배된 하나 이상의 식물을 선택하는 단계를 포함하는 방법에 의해서 생성된 변형된 자기/자율 질소 고정 프로파일을 나타내는 식물이 제공되며, 여기서 선택된 식물은 각각 증진된 질소 고정을 나타내고, 선택된 하나 이상의 식물은 각각 SEQ. ID. No. 1, SEQ. ID. No. 2, SEQ. ID. NO. 3, SEQ. ID. NO. 46, SEQ. ID. NO. 47, SEQ. ID. NO. 48 또는 SEQ. ID. NO 49를 암호화하는 재조합 핵산 서열을 포함한다.

일부 구체예들에서, 본 개시의 원리에 따라서, 식물 세포를 SEQ. ID. NO. 1, SEQ. ID. NO. 2, SEQ. ID. NO. 3, SEQ. ID. NO 46, SEQ. ID. NO 47, SEQ. ID. NO 48 또는 SEQ. ID. NO. 49 중 하나를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포를 토마토 엽록체 발현에 대해 최적화된 적어도 하나의 합성 코딩 DNA 서열(sCDS)과 접촉시키는 단계; 식물 세포로부터 하나 이상의 식물을 재생시키는 단계; 및 증진된 질소 고정을 나타내는 식물 세포로부터 재배된 하나 이상의 식물을 선택하는 단계를 포함하는 방법에 의해서 생성된 증진된 질소 고정을 나타내는 식물이 제공되며, 여기서 상기 하나 이상의 식물은 SEQ. ID. NO. 1, SEQ. ID. NO. 2, SEQ. ID. NO. 3, SEQ. ID. NO 46, SEQ. ID. NO 47, SEQ. ID. NO 48 또는 SEQ. ID. NO. 49를 암호화하는 재조합 핵산 서열과 sCDS를 포함한다.

일부 구체예들에서, 본 개시의 원리에 따라서, 하나 이상의 식물 세포를 제1 프로모터와 작동가능하게 연결된 Nif-H 유전자와 같은 엽록체(색소체) 축적을 위한 식물 트랜짓 펩티드 또는 신호를 가진 키메라 Nif 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포를 제2 프로모터와 작동가능하게 연결된 Nif-D 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포를 제3 프로모터와 작동가능하게 연결된 Nif-K 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포로부터 하나 이상의 식물을 재생시키는 단계; 및 식물 세포로부터 재배된 하나 이상의 식물을 선택하는 단계를 포함하는 방법에 의해서 생성된 증진된 질소 고정을 나타내는 식물이 제공되며, 여기서 선택된 식물은 각각 증진된 질소 고정을 나타내고, 선택된 식물은 각각 Nif-H 유전자를 암호화하는 재조합 핵산 서열, Nif-D 유전자를 암호화하는 재조합 핵산 서열 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함한다. 일부 구체예에서, Nif-H 유전자는 SEQ ID. NO. 29, SEQ ID. NO. 32, SEQ ID. NO. 35 및 SEQ ID. NO. 38로 필수적으로 구성된 군으로부터 선택된다. 일부 구체예에서, Nif-D 유전자는 SEQ ID. NO. 30, SEQ ID. NO. 33, SEQ ID. NO. 36 및 SEQ ID. NO. 39로 필수적으로 구성되는 군으로부터 선택된다. 일부 구체예에서, Nif-K 유전자는 SEQ ID. NO. 31, SEQ ID. NO. 34, SEQ ID. NO. 37 및 SEQ ID. NO. 40으로 필수적으로 구성되는 군으로부터 선택된다. 일부 구체예에서, 제1, 제2 및 제3 프로모터는 SEQ. ID. NOs. 41-45로 필수적으로 구성되는 군으로부터 선택된다.

또한, 본 개시는 트랜스제닉 식물-유래 상업적 제품에 관한 것으로서, 이것은 본 개시에서 설명된 방법에 따른 트랜스제닉 식물로부터 유래된다.

또한, 토양에서 질소의 전체 농도를 감소시키는 방법이 본 개시의 원리에 따라서 설명된다. 이 방법은 본 개시에서 설명된 적어도 하나의 트랜스제닉 식물을 질소의 수준이 감소되어야 하는 토양과 접촉시켜 두는 단계; 및 식물이 성장하도록 하여 토양에서 질소의 전체 농도를 감소시키는데 있어서 이들의 대사 결과로 토양으로부터 얻어진 질소를 고정하는 단계를 포함한다. 이들 및 다른 원리들은 도면 및 상세한 설명을 사용하여 더 설명된다.

도 1은 니트로게나아제 복합체의 도식적 구조를 도시한다.
도 2는 토마토 엽록체 형질전환 동안 색소체에 삽입되도록 구성된 플라스미드를 생성하기 위하여 사용된 단계들의 도식적 표현을 도시한다.
도 3은 토마토 엽록체 형질전환 및 Nif-H, Nif-D 및 Nif-K 유전자의 발현을 위한 플라스미드를 생성하기 위한 단계들의 도식적 표현을 도시한다(PGE 011).
도 4는 단일 오페론으로서 클로닝되고 C. reinhardtii 엽록체에 삽입된 Nif-H, Nif-D 및 Nif-K 유전자를 도시한다.
도 5는 발아 3주 후 토마토 식물의 발생을 도시한다.
도 6은 식물 플라스미드에 Nif-H의 축적을 도시한다.
도 7은 식물 형질전환 플라스미드의 도식적 구조를 도시한다.

본 개시는 질소를 고정하는 것이 가능하게 된 트랜스제닉 식물, 이러한 식물로부터 생산된 제품, 트랜스제닉 식물의 제조 방법 및 트랜스제닉 식물을 사용하여 토양이나 물에서 질소를 감소시키는 방법에 관한 것이다.

본 개시는 본 개시의 일부분을 형성하는 첨부한 도면을 함께 보면서 본 개시의 이후의 상세한 설명을 참조함으로써 더 쉽게 이해될 수 있다. 본 개시는 여기 설명되고 및/또는 도시된 특정 조성물, 장치, 방법, 조건 또는 변수에 제한되지 않으며, 여기서 사용된 용어는 단지 예시의 방식으로 특정한 구체예들을 설명하기 위한 목적이며, 청구된 개시의 제한인 것으로 의도되지 않는다는 것이 이해되어야 한다. 또한, 첨부된 청구항을 포함하여 본 명세서에서 사용된 단수형 "한" 및 "그"는 복수를 포함하며, 특정한 수치값의 언급은 문맥상 분명히 다른 의미가 아니라면 적어도 그 특정한 값을 포함한다. 범위는 "약" 또는 "대략" 하나의 특정한 값에서부터 및/또는 "약" 또는 "대략" 다른 특정한 값까지로서 여기서 표현될 수 있다. 이러한 범위가 표현될 때, 다른 구체예는 하나의 특정한 값에서부터 및/또는 나머지 하나의 특정한 값까지를 포함한다. 유사하게, 선행사 "약"을 사용하여 값들이 근사값으로 표현될 때, 그 특정한 값은 다른 구체예를 형성한다는 것이 이해될 것이다.

본 명세서와 첨부된 청구항의 취지에 있어서, 달리 나타내지 않는다면, 원료의 양을 표시하는 모든 숫자, 재료의 퍼센트나 비율, 반응 조건, 및 명세서와 청구항에서 사용된 다른 수치값들은 모든 예에서 용어 "약"에 의해서 변형된다는 것이 이해되어야 한다. 따라서, 달리 나타내지 않는다면, 이후의 명세서 및 첨부된 청구항에서 제시된 수치 변수들은 본 개시에 의해서 얻고자 하는 바람직한 특성에 따라서 변할 수 있는 근사값이다. 청구항의 범위에 대한 등가물 개념의 적용을 제한하려는 시도로서는 아니지만 적어도 각 수치 변수는 보고된 의미있는 숫자에 비추어 통상의 라운딩 기술을 적용함으로써 적어도 이해되어야 한다.

여기 제시된 수치 범위 및 변수에도 불구하고, 본 개시의 넓은 범위는 근사값이지만, 특정 실시예에서 제시된 수치값은 가능한 정확히 보고된다. 그러나, 어떤 수치값은 각자의 시험 측정에서 발견된 표준 편차로부터 본질적으로 생기는 어떤 오차를 본래 함유한다. 더욱이, 여기 개시된 모든 범위는 그 안에 포함된 어떤 및 모든 하위범위를 포괄하는 것으로 이해되어야 한다. 예를 들어 "1 내지 10"의 범위는 1의 최소값과 10의 최대값 사이의(포함하는) 어떤 및 모든 하위범위, 즉 1 이상의 최소값과 10 이하의 최대값을 갖는 어떤 및 모든 하위범위, 예를 들어 5.5 내지 10을 포함한다.

본 출원에서 표제들은 어떤 식으로도 본 개시를 제한하려는 의도가 아니다. 어떤 하나의 표제 아래의 구체예들은 어떤 다른 표제 아래의 구체예들과 함께 사용될 수 있다.

본 명세서 및 첨부된 청구항에서 사용된 대로, 단수형 "한" 및 "그"는 하나의 지시대상에 분명히 그리고 명백히 제한되지 않는다면 복수의 지시대상을 포함한다. 따라서, 예를 들어 "척추간 디스크 이식물"이란 언급은 하나, 둘, 셋 또는 그 이상의 척추간 디스크 이식물을 포함한다.

다음의 논의는 조성물, 유전자 과정, 스스로 질소를 고정할 수 있도록 식물을 형질전환하기 위해서 필요한 관련된 트랜스제닉 세포 및 결과의 트랜스제닉 식물, 뿐만 아니라 본 개시의 원리에 따른 질소 시비에 대한 필요 또는 요구를 감소시키기 위한 트랜스제닉 식물(유전자 변형된 유기체(GMO))의 사용 방법에 관한 설명을 포함한다. 대안적인 구체예들도 또한 개시된다. 이제 본 개시의 특정한 구체예들이 상세히 언급될 것이며, 이들의 실시예들이 첨부된 도면에 도시되고 실시예에서 논의된다. 본 개시는 예시된 구체예들과 함께 설명되지만, 본 개시를 이들 구체예에 제한한다는 의도가 아니라는 것이 이해될 것이다. 반대로, 본 개시는 첨부된 청구항에 의해서 정의된, 본 개시 내에 포함될 수 있는 모든 대안, 변형, 및 등가물을 포괄하도록 의도된다.

본 개시의 조성물 및 방법으로 다시 돌아가서, 본원에서 제공되는 조성물 및 방법은 이종성 니트로게나아제의 활성을 통해서 식물에서 유기 질소를 생성하기 위한 것이며, 이로써 식물은 암모니아를 생성할 수 있다.

일부 구체예들에서, 본 개시에 따라서, 표적 식물은 Nif-H, Nif-D 및/또는 Nif-K(SEQ. ID. NOs. 1-3 및/또는 29-40)를 가진 시아노박테리아로부터의 유전자 서열을 함유하는 DNA로 유전적으로 형질전환된다. 대안으로서, 표적 식물은 식물 색소체에서 발현될 수 있는 특정 조건하의 다른 박테리아로부터의 유사한 유전자로 유전적으로 형질전환될 수 있다(프로모터 및 출발 번역 서열, 뿐만 아니라 상동성 재조합 서열). 이것은 색소체(엽록체)에 제한되지 않는다. 도 3 및 도 4는 색소체 형질전환에 사용된 플라스미드를 도시한다.

표적 식물은, 신규 유전자들 또는 유전자가 도입될 예정인 식물 세포 또는 조직을 말하며, 시아노박테리아로부터의 Nif-H, Nif-D 및 Nif-K의 유전자 시퀀싱을 함유하는 "신규" DNA로 유전적으로 형질전환될 것이다(즉 Cyanobacterium Anabaena PCC 7120(SEQ. ID. Nos. 1-2)(또는 다른 시아노박테리아(SEQ I.D. NOs. 29-40), 또는 다른 박테리아(색소체에 "효소"로서 Nif 단백질이 축적되는 특정 조건에서만). 이들 유전적 특징을 갖도록 형질전환된 식물은 자기 또는 자율 질소 고정 기구로서 기능하는 능력을 가진다. 자기 또는 자율 질소 고정은 질소를 암모니아로 고정할 수 있는 세포(식물 세포), 식물, 또는 뿌리나 잎 같은 식물 일부를 말한다. 이것은 리조비움과 같은 다른 유기체와의 공생에 의한 것이 아니다. 대안적인 서열이 표적 식물에 사용될 수 있다. 예를 들어, Nif-D는 다른 Nif-D의 각각의 코딩 서열로 대체될 수 있으며, Nif-K 및 Nif-H에도 각각 동일한 규칙이 적용된다.

일단 식물이 여기 설명된 대로 유전적으로 변형되면, 자기 생물학적 질소 고정을 할 수 있고, 식물에서 질소 고정의 생성을 위해 이용될 수 있다. 새로 조작된 식물은 식물 사용이나 동물 사용을 위해 이용가능한 형태로 질소를 생성할 것이다.

토마토 식물(리코페르시쿰 종)(lycopersicum sp.) 또는 담배 식물(니코타나 타바콤)(Nicotana tabacom) 같은 어떤 식물도 본 발명을 이용할 수 있다. 이들은 상업적 제품으로서, 농업용 파종물로서, 농업용 근경으로서(접목 기술 사용) 또는 식물 연구를 위한 모델 식물 유기체로서 사용될 수 있다.

일부 구체예들에서, Nif 유전자(코딩 서열 또는 mRNA, RNA 또는 DNA라고 한다)의 공급원은 박테리아 캐리어일 수 있다. Nif 유전자는 시아노박테리움처럼 특유의 광합성 박테리아에서 발견된다. 식물 세포, 색소체 또는 엽록체에서 Nif-H 유전자의 발현은 Nif-H 단백질이 Nif D-K 복합체에 대한 의무적 전자 도너로서 기능적으로 작용할 수 있는 활성 효소로 조립되는 것을 허용한다. 이 발현은 Nif-H가 니트로게나아제 리덕타아제로서 기능하도록 한다. 일부 구체예에서, Nif 유전자는 합성에 의해 생성되며 자연에서 발견되지 않는다. 예를 들어, SEQ. ID. NOs. 29-40 참조.

식물 세포 또는 엽록체에서 Nif-D 유전자 및 Nif-K 유전자의 발현은 Nif-D-K 단백질의 축적을 허용한다. 식물 세포 및/또는 색소체에서 상기 유전자 발현은 니트로게나아제 Mo-Fe 단백질로서 알려져 있으며, 니트로게나아제 서브유닛으로 기능한다. 상기의 조합은 그것의 질소 필요량의 일부 또는 전부를 고정/제공할 수 있는 식물을 생성할 것이다. 질소를 고정할 수 있는 식물인 공생 박테리아에 의존할 필요가 없고, 화학적 또는 유기 질소 공급원에 의존할 필요가 없기 때문에 더욱 강건할 것이다. 식물 세포에서 Nif-H, Nif-D 및 Nif-K 유전자의 사용은 질소 고정에서 박테리아 또는 원핵생물 핵심 경로의 기능을 모방한다.

Nif-H, Nif-K 및 Nif-D의 어느 조합으로 Nif 단백질을 축적한 식물 세포는 질소를 소비가능한 형태(암모니아(NH₃)) 중 하나로, 다음에 암모늄(NH₄)으로 고정할 것이다. 상기 중 하나 또는 모두의 핵으로부터의 발현은 그것을 식물 색소체에 표적화하여 도 6에 도시된 대로 기능적 Nif 복합체(Nif-H 및 Nif-D-K)의 시간에 따른 축적에 대한 보다 나은 제어를 허용할 수 있다.

변형된 자기/자율 질소 고정 프로파일을 가진 트랜스제닉 식물을 생성하는 방법이 제공되며, 여기서 트랜스제닉 식물은 그 식물이 질소 고정에 대한 박테리아 또는 원핵생물 핵심 경로를 모방할 수 있도록 Nif-H, Nif-D 및 Nif-K 유전자의 조합을 그것의 게놈, 또는 그것의 색소체 게놈에 포함한다. 이 방법은 숙주 식물의 게놈에 재조합 Nif-H, Nif-D 및 Nif-K 유전자를 삽입함으로써 숙주 식물을 형질전환하는 것을 포함하며, 여기서 Nif-H, Nif-D 및 Nif-K 유전자는 각각 프로모터 서열, 터미네이터 서열 및 선택적으로 표적화 신호 또는 트랜짓 펩티드를 암호화하는 DNA 서열에 작동가능하게 연결되고, 이들은 모두 상기 숙주 식물에서 활성이다. 일부 구체예에서, 재조합 Nif-H, Nif-D 및 Nif-K 유전자는 SEQ ID. NO. 1에 포함된다. 일부 구체예에서, 재조합 Nif-H, Nif-D 및 Nif-K 유전자는 SEQ ID. NO. 2에 포함된다. 일부 구체예에서, 재조합 Nif-H, Nif-D 및 Nif-K 유전자는 SEQ ID. NO. 3에 포함된다. 당업자에 의해서 인정되는 대로, SEQ ID. NOs. 1 및 2가 각각 SEQ ID. NO. 4A로 확인된 프로모터를 포함하기 때문에, SEQ. ID. NO. 1 또는 SEQ. ID. NO. 2를 사용할 때는 숙주 식물에 추가의 프로모터를 형질전환할 필요가 없다. 마찬가지로, SEQ ID. NO. 3도 SEQ ID. NO. 4B로 확인된 프로모터를 포함하기 때문에, SEQ. ID. NO. 3을 사용할 때는 숙주 식물에 추가의 프로모터를 형질전환할 필요가 없다. 또는 달리, 이 서열들은 트랜짓 펩티드를 부가함으로써 핵에 형질전환될 수 있으며, 생성물은 색소체에 축적될 것이다.

일부 구체예들에서, 본 개시의 원리에 따라서, 식물이 질소 고정에 대한 박테리아 또는 원핵생물 핵심 경로를 모방할 수 있도록 Nif-H, Nif-D 및 Nif-K 유전자의 조합을 그것의 게놈(색소체 게놈)에 포함하는 변형된 자기/자율 질소 고정 프로파일을 가진 트랜스제닉 식물을 생성하는 방법이 제공되며, 이 방법은 하나 이상의 식물 세포를, 예를 들어 rrn16 프로모터와 같은 프로모터에 작동가능하게 연결된 Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포로부터 하나 이상의 식물을 재생시키는 단계; 및 식물 세포로부터 재배된 하나 이상의 식물을 선택하는 단계를 포함하며, 여기서 선택된 식물은 각각 증진된 질소 고정을 나타내고, 선택된 식물은 각각 rrn16 프로모터에 작동가능하게 연결된 재조합 핵산 서열을 암호화하는 재조합 핵산 서열을 포함한다. 이 방법은 도 7의 부분 B에 도시된다. 한 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열은, 예를 들어 rrn16 프로모터와 같은 단일 프로모터에 작동가능하게 연결된다. 일부 구체예에서, 재조합 핵산 서열은 SEQ. ID. NOs. 1-3 및 SEQ. ID. NO. 49 중 적어도 하나의 적어도 일부를 포함한다.

일부 구체예들에서, 본 개시의 원리에 따라서, 식물이 질소 고정에 대한 박테리아 또는 원핵생물 핵심 경로를 모방할 수 있도록 Nif-H, Nif-D 및 Nif-K 유전자의 조합을 그것의 게놈에 포함하는 변형된 자기/자율 질소 고정 프로파일을 가진 트랜스제닉 식물을 생성하는 방법이 제공되며, 이 방법은 하나 이상의 식물 세포를 프로모터에 작동가능하게 연결된 Nif-H 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포를 프로모터에 작동가능하게 연결된 Nif-D 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포를 프로모터에 작동가능하게 연결된 Nif-K 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계; 식물 세포로부터 하나 이상의 식물을 재생시키는 단계; 및 식물 세포로부터 재배된 하나 이상의 식물을 선택하는 단계를 포함하며, 여기서 선택된 식물은 각각 증진된 질소 고정을 나타내고, 선택된 식물은 각각 Nif-H 유전자, Nif-D 유전자 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함하고, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 핵산 서열은 각각 프로모터에 작동가능하게 연결된다. 이 방법은 도 7의 부분 A에 도시된다.

일부 구체예들에서, Nif 유전자(Nif-H, Nif-D, Nif-K)를 암호화하는 재조합 핵산 서열은 SEQ. ID. NOs. 29-40로 필수적으로 구성되는 군의 적어도 3개를 포함한다. 일부 구체예에서, Nif-H 유전자를 암호화하는 재조합 핵산 서열은 SEQ. ID. NOs. 29, 32, 35 및 38로 필수적으로 구성되는 군으로부터 선택된다. 일부 구체예에서, Nif-D 유전자를 암호화하는 재조합 핵산 서열은 SEQ. ID. NOs. 30, 33, 36 및 39로 필수적으로 구성되는 군으로부터 선택된다. 일부 구체예에서, Nif-D 유전자를 암호화하는 재조합 핵산 서열은 SEQ. ID. NOs. 31, 34, 37 및 40으로 필수적으로 구성되는 군으로부터 선택된다. 일부 구체예에서, 프로모터는 SEQ. ID. NOs. 41-45로 필수적으로 구성되는 군으로부터 선택된다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 핵산 서열은 각각 자연에서 발견되는 단일 유기체에 존재한다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 핵산 서열은 각각 자연에서 발견되는 상이한 유기체에 존재한다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 핵산 서열은 각각 합성이며 자연에서 발견되지 않는다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자 중 하나를 암호화하는 핵산 서열 중 적어도 하나는 자연에서 발견되고, Nif-H, Nif-D 및 Nif-K 유전자 중 하나를 암호화하는 핵산 서열 중 적어도 하나는 합성이며 자연에서 발견되지 않는다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자 중 하나를 암호화하는 핵산 서열은 각각 터미네이터를 포함한다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자 중 하나는 Nif-H, Nif-D 및 Nif-K 유전자 중 다른 것과 상이한 프로모터에 연결된다. 일부 구체예에서, 프로모터는 SEQ. ID. NO. 4 및/또는 SEQ. ID. NO. 28을 포함한다. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자는 각각 예를 들어 rrn116 프로모터와 같은 동일한 종류의 프로모터에 연결된다.

일부 구체예들에서, 선행한 문단들 중 어느 하나에서 논의된 방법은 다음 단계들 중 적어도 하나를 포함한다: 표적 유기체를 확인하는 단계; 표적 유기체가 핵 형질전환 또는 색소체 형질전환에 보다 적합한지의 여부를 결정하는 단계; Nif 유전자를 포함하는 도너 유기체의 단백질 서열을 확인하는 단계; 보편적 유전자 코돈을 사용하여 단백질 서열을 DNA로 역번역하는 단계; 코딩 DNA 서열(CDS)을 표적 유기체(즉 토마토 식물)에 대해 최적화하는 단계; 합성 CDS를 합성하는 단계; 프로모터 터미네이터를 부가하는 단계; CDS를 안정한 또는 일시적 형질전환을 위해 식물에 형질전환하는 단계; 및 ARA, 15N 고정 동위원소 통합 또는 질소 고갈 배지에서 성장할 수 있는 능력 중 하나 이상의 분석에 의해 니트로게나아제 활성을 분석하는 단계. 일부 구체예에서, Nif-H, Nif-D 및 Nif-K 유전자는 모두 하나의 오페론으로서 동일한 프로모터에, 예컨대 예를 들어 rrn116 프로모터에 연결된다.

일부 구체예들에서, 도너 유기체는 광합성 능력이 있으며 질소 고정 능력을 가진 단세포 시아노박테리아를 포함한다. 일부 구체예에서, 도너 유기체의 Nif 유전자는 BlastN에 의해, 혹은 문헌 검토에 의해 확인된다. 일부 구체예에서, 확인된 Nif 유전자는 Nif-H, Nif-D 및 Nif-K 유전자를 포함한다. 일부 구체예에서, 표적 유기체에 대한 코딩 DNA 서열은, 가장 빈번한 코돈만을 사용하고 다른 것들은 무시하는 공통 코돈 활용 최적화장치와는 달리, 덜 빈번하게 사용된 코돈을 무시하지 않도록 Rubisco의 ad hoc 코돈 활용을 사용하여 최적화된다(SEQ ID. NOs. 35-37). 일부 구체예에서, 표적 유기체에 대한 코딩 DNA 서열은 표준 코돈 최적화장치 및 Rubisco SEQ ID. NOs. 29-36의 최고 활용을 사용하여 최적화된다. 일부 구체예에서, 표적 유기체에 대한 코딩 DNA 서열은 총 엽록체 공지 활용 코돈 SEQ ID NOs. 38-40을 사용하여 최적화된다.

본 개시의 원리에 따라서 프로모터 서열 및 터미네이터 서열에 작동가능하게 연결된 재조합 Nif-H, Nif-D 및 Nif-K 유전자를 그것의 게놈에 포함하는 변형된 질소 고정 생성 프로파일을 가진 트랜스제닉 식물이 제공된다.

도 1은 니트로게나아제 복합체의 도식적 구조를 도시한다. 그것은 보조인자로서 4개 철 원자와 함께 2개의 Nif-H 단백질에 의해 형성된다(호모다이머). Nif-K 단백질은 보조인자로서 몰리브데늄과 함께 2개의 Nif-D 서브유닛과 2개의 Nif-K 서브유닛을 가진 헤테로다이머를 형성한다.

본 개시의 다른 양태에서, 자기 또는 자율 생물학적 질소 고정(sBNF) 작물 식물이 제공된다. 예를 들어, 토마토 식물(리코페르시콘 종)(Rycopersicon sp.)은 니트로게나아제 리덕타아제 효소(Nif-H 및 Nif-D-K)에 대한 발현된 유전자를 지닐 수 있고, 이로써 박테리아 경로를 모방할 수 있다. 니트로게나아제 리덕타아제 효소(Nif-H 및 Nif-D-K)에 대한 핵산 서열은 SEQ. ID. NOs. 1 및 2로서, 도 6에 도시된 대로, SEQ. ID. NO. 29로서 제공된다.

본 개시의 원리에 따른 다른 구체예에서, 원래 박테리아 서열을 대체할 수 있는 코돈을 사용하여 표적 식물 소기관(색소체)에서 Nif 유전자 코딩 서열이 최적화된 작물 식물 및/또는 어떤 다른 식물에서 자기 또는 자율 BNF가 제공된다. 예를 들어, 토마토 식물(리코페르시콘 종)(lycopersicon sp.)은 니트로게나아제 리덕타아제 효소에 대한 발현된 유전자, 즉 Nif-H 및 NifD- K를 지닐 수 있으며, 이로써 박테리아 경로를 모방할 수 있다.

도 2 및 3은 토마토 엽록체 형질전환을 위한 플라스미드를 위한 플라스미드를 생성하는데 사용된 단계들의 도식적 표현을 도시한다(PGE 003): 폴리머라아제 연쇄 반응(PCR)을 사용하여 특정 프라이머 또는 올리고머(표 1)에 의해서 DNA를 증폭했고, 이어서 효소 제한 소화시키고 플라스미드 상부 부분에 결찰시켰다. 4 단계 후, 생성물은 tRNA-FM 및 t-RNA-G로 알려진 2개의 엽록체 상동성 부위를 함유하며, PsbA의 터미네이터에 의해서 몇몇 특유의 클로닝 부위가 이어진다.

도 3은 토마토 엽록체 형질전환 및 토마토 식물 형질전환에 필요한 Nif-H, Nif-D 및 Nif-K 유전자의 발현을 위한 플라스미드를 생성하기 위한 단계들의 도식적 표현을 도시한다. 폴리머라아제 연쇄 반응(PCR)을 사용하여 특정 프라이머 또는 올리고머(표 1)에 의해서 DNA를 증폭했고, 이어서 효소 제한 소화시키고 플라스미드에 결찰시켰다. 한 단계 또는 두 단계 후, 플라스미드는 Nif 유전자를 함유하며 코딩 서열이 작성된다. 3개 유전자, 즉 Nif 유전자, 리포터 유전자 및 선택 마커, 예컨대 예를 들어 aadA가, 예를 들어 제한 효소와 같은 절단제를 사용하여 하나의 클러스터로서 클로닝된다.

도 4는 C. reinhardtii의 엽록체 또는 다른 엽록체에 삽입된 단일 오페론으로서 Nif-H, Nif-D 및 Nif-K 유전자를 도시한다. 엽록체는 원핵생물 유전자 발현 시스템을 함유하며, 이것은 오페론이 발현되도록 한다. 광합성에 의해 기증된 전자와 ATP가 니트로게나아제 효소가 작용하도록 허용하여, 그 결과 NH₄가 생성된다. 조류 페레독신(Fd)가 광학계 I(PSI)로부터 니트로게나아제의 Nif-H/Fe-단백질로 전자 전달을 매개한다. 본 개시는 Nif 복합체가 그것의 원래 공급원으로 인하여 산소에 덜 민감하다는 것과 Fe-단백질(Nif-H)이 자율/자기 질소 고정에 도움이 되도록 전자를 Nif-K로 앞으로 전달할 수 있다는 것을 시사한다.

본 개시의 원리는, 예를 들어 쌀, 옥수수, 감자, 스쿼시 멜론, 담배, 목화 아라비도프시스, 및 사과, 체리, 호두 같은 나무들, 및 또한 녹조류, 뿐만 아니라 다른 식물들과 같은 어떤 식물에도 적용될 수 있다고 생각된다. 예를 들어, 이 기술을 클라미도모나스(녹조류)에 적용하기 위해서 코딩 서열이 SEQ. ID. NO. 3을 갖는 합성 유전자 합성에 의해서 최적화되었다.

본 개시의 다른 구체예는 질소 시비에 대한 필요 또는 요구르 감소시키기 위한 트랜스제닉 식물(유전자 변형된 유기체(GMO))의 사용 방법을 제공한다. 본 개시의 다른 구체예는 질소 시비를 대체하기 위한 트랜스제닉 식물(유전자 변형된 유기체(GMO))의 사용 방법을 제공한다(예를 들어, GMO는 식물 비료로서 사용될 수 있다).

아래 제공되는 실시예들은 스스로 질소를 고정하는 능력을 달성하기 위한 식물, 즉 토마토 식물의 유전자 조작을 설명한다.

실시예: 토마토 색소체 형질전환을 위한 플라스미드 생성

본 개시에서, 원핵생물 또는 박테리아 니트로게나아제 및 BNF가 식물에서 암모니아 및/또는 암모늄의 생성에 이용된다. 이들 트랜스제닉 식물은 BNF에 대한 코드를 가진 플라스미드로 일단 형질전환되면 그것의 질소 요구의 전부 또는 일부를 생성할 수 있는 능력을 가진다. 토마토, 쌀 또는 밀 같은 사람의 소비에 적합하고, 다른 식물과 조류를 포함하여 꽃, 풀 및 나무 같은 원예 식물로서 적합한 작물이 또한 자율/자기 질소 고정을 위해서 형질전환될 수 있다. 이들 플라스미드는 자연에 존재하지 않으므로, 세포/식물이 자율/자기 질소 고정을 가능하게 하는 인공 플라스미드를 생성하기 위해 합성 생물학이 사용된다. 인공 플라스미드는 CDS 및 조절 서열의 변형을 함유하며, 역시 예를 들어 식물에서 니트로게나아제의 발현 및 축적이라는 새로운 일을 수행할 수 있다.

실시예: 유전자 물질의 분리

식물 DNA 분리: 3-4주 된 어린 토마토 식물을 사용하여 Dneasy Plant Mini Kit(Qiagene Germany)를 사용해서 게놈(뉴클레아제) DNA와 색소체 DNA를 분리했다. 순수한 DNA를 사용하여 관심의 DNA 서열에 대한 PCR 증폭을 완료했다.

SEQ. ID. NOs. 5, 6, 7 및 8(표 1 참조)를 사용한 특정 PCR 반응에 의해서 토마토 DNF를 증폭했다. 이것은 토마토 재조합 부위(SEQ. ID. NOs. 5, 6, 7 및 8(표 1 참조) 및 PsbA 신호의 종결을 가져왔다(SEQ. ID. NOs. 17 및 18). 모든 3개의 PCR 생성물은 (토마토) 색소체 DNA로부터이다.

PsbA는 D1 단백질(PsbA라고도 알려져 있다)에 대한 유전자로서, 광학계 II 반응 중심의 반응 핵심을 형겅한다. 그것은 구성적으로 발현된 유전자로서 잘 알려져 있다.

다음에, PCR 증폭된 DNA를 제한 효소에 의한 소화에 사용하여 SacI-SacII, XhoI-KpnI, 및 BamHI-AscI에서 응집성 단부 또는 점착성 단부를 생성했으며, 이들은 각각 PCR 생성물 SEQ. ID. NOs. 5, 6, 7, 8, 17 및 18에 각각 부착된다.

응집성 단부 또는 점착성 단부는 제한 효소가 3' 또는 5' 오버행을 생성할 때 사용되는 용어이다. 이들 오버행은 대부분의 경우 팔리드롬형(대칭)이다. 각 응집성 단부는 그것의 상보성 서열과 배타적으로 결찰될 것이다. 결찰된 또는 결찰은 DNA 단편들을 함께 "붙이는" T4-DNA 리가아제 같은 효소에 의해서 일반적으로 수행되는 DNA 분자의 두 단부의 공유 결합 반응을 말하지만, DNA 또는 DNA 리가아제에만 제한되는 것은 아니다.

PCR 제한된 DNA는 T4-DNA 리가아제 효소(NEB.USA)와 함께 인큐베이션함으로써 도 2에 도시된 대로 세 가지 상태 반응으로 pBluescript 플라스미드에 클로닝되었고, 재조합된 공장 조건에서 완성되었다.

비-팔린드롬형 다중 클로닝 부위(MCS)를 위한 특유의 제한을 부가하기 위해서, PCR에 의해서 pPZP-RCSII의 MCS를 증폭하여 도 2 및 도 7에 도시된 대로 XhoI-SacI 부위에 클로닝했다.

PGE 003으로 명명된 신규 플라스미드는 AMP-R 또는 BLA 유전자와 상동성 재조합을 위한 pBluescript의 ORI 및 토마토 엽록체 부위(Tom Chl1 및 도 2의 2), 및 pPZP-RCSII의 MCS 인공 DNA 서열을 가진 토마토로부터의 PsbA 터미네이터로 구성된다.

이 과정을 사용하여 생성된 PGE 003 플라스미드는 특유의 다중 클로닝 부위 MCS를 가지며, 제한 부위를 가진 특유의 DNA는 다음의 제한 효소로 구성된 클로닝에서 제한 효소의 사용을 허용한다: XhoI, Pi-PspI, I-CeuI, I-SceI, I-PpoI, 및 AscI, 이것은 순서 및 배향으로 관심의 유전자 클로닝을 허용된다. Pi-PspI, I-CeuI, I-SceI 및 I-PpoI는 팔린드롬형이 아니므로, 점착성 단부는 대칭이 아니다. 따라서, 결찰은 한 방향으로만 일어난다.

실시예: Nif 유전자의 클로닝 및 발현

SEQ. ID. NOs. 19 및 20을 가진 올리고들을 사용한 특정 PCR 반응에 의해서 토마토 DNA를 증폭하고, PGE 006을 생성하는 pSAT6-MCS의 AgeI-BamHI 부위에 클로닝했다. 이것은 Pi-PspI 부위 사이에 관심의 DNA를 가진 임시 플라스미드이다. 이 클로닝 단계에 의해서 토마토 DNA로부터의 rrn16의 프로모터가 클로닝되었다.

pSAT-벡터는 호밍 엔도뉴클레아제를 사용하는 일련의 벡터(플라스미드)이다. 호밍 엔도뉴클레아제는 일반적으로 긴 인식 부위를 가진 제한 효소이다. 이 특징은 이들을 "희귀 절단제"로 만드는데, 무작위 선택된 DNA에서 이들의 절단 빈도가 드물기 때문이다. 예를 들어, pSAT4는 I-SceI AGTTACGCTAGGGATAACAGGGTAATATAG SEQ. ID. No. 25(Genbank SEQ. ID. No. DQ005466로서 이용가능)에 대해 2개의 반복부 부위를 가지며, 이들은 규칙적 제한 효소의 6-8과 비교하여 30 염기이다.

SEQ. ID. NOs. 9 및 10(표 1 참조)을 가진 올리고들을 사용한 특정 PCR 반응에 의해서 시아노박테리아 ATCC7120 DNA에서 PCR을 증폭하고, rrn16 프로모터의 제어하에 Nif-H 유전자를 가진 플라스미드인 PGE 007을 생성하는 (식물 유전자 조작을 위한 플라스미드)(PGE) 006의 (제한 부위) BglII-NotI 부위에 클로닝했다.

rrn16-프로모터는 색소체의 잘 알려진 발현 프로모터인 리보솜 RNA 16S에 대한 색소체 유전자의 5' DNA 서열을 말하며, 본 개시에서 사용되지만, 다른 유도성 프로모터들도 가능하다. 즉, 프로모터는 식물이 자율/자기 질소 생산 및/또는 증진된 질소 흡수, 동화 또는 사용 용량을 갖도록 할 수 있는 것이 바람직한 어떤 조건에서 유도성인 것으로 선택된다. 예를 들어, 적합한 프로모터는, 제한은 아니지만, 질소원의 적용에 의해 유도되는 것들, 스트레스 유도성, 상처 유도성 또는 다른 화학물질의 적용에 의해 유도되는 것들을 포함할 수 있다. 본 개시의 유전자 구성물을 함유하는 트랜스제닉 식물은 대조군 식물에 비해서 증진된 작물학적 특징을 나타낸다. 증진되는 특정한 작물학적 특징은 일반적으로 프로모터의 성질에 따르며, 증진된 스트레스 관용성 및/또는 더 효과적인 질소 흡수, 저장 또는 대사를 포함할 수 있고, 이것은 본 발명의 식물이 거의 질소 비료 투입 없이 질소 결핍 조건에서 재배되도록 허용하거나, 또는 정상 성장 조건에서 더 빠른 성장, 더 많은 식물생장 및/또는 생식 수율을 허용한다.

SEQ. ID. NOs. 11 및 12(표 1 참조)를 가진 올리고들을 사용한 특정 PCR 반응에 의해서 시아노박테리아 ATCC7120 DNA에서 PCR을 증폭하고, Nif-D 유전자를 가진 플라스미드인 PGE 008을 생성하는 pSAT5-MCS의 AgeI-XhoI 부위에 클로닝했다.

SEQ. ID. NOs. 13 및 14(표 1 참조)를 가진 올리고들을 사용한 특정 PCR 반응에 의해서 시아노박테리아 ATCC7120 DNA에서 PCR을 증폭하고, Nif-D 및 Nif-K 유전자를 가진 PGE 008 생성 플라스미드의 XhoI-NotI 부위에 클로닝했다(도 5 상부 중앙). SEQ. ID. NOs. 23 및 24(표 1 참조)를 가진 올리고들을 사용한 특정 PCR 반응에 의해서 pPZP-RCSII DNA에서 PCR을 증폭하고, aadA 유전자를 가진 PGE 005를 생성한 pAST4의 AgeI-XhoI 부위에 클로닝했다(도 4 상부 오른쪽). aadA-는 식물 형질전환을 위한 리포터 유전자이다. 이 유전자는 아데닐화에 의해 스펙티노마이신과 스트렙토마이신을 비활성화하는 아미노글리코시드 3' 아데닐일트랜스페라아제 효소를 암호화하고, 엽록체 리보솜과의 결합을 방지하여, 식물이 항생제를 함유하는 배지에서 성장할 수 있도록 한다. 또는 달리, 컴퓨터에서 기본적 설계 후, 전체 핵산 서열이 상업적 소프트웨어에 제출되고, 서로 연결된 중첩된 연속 염기들을 가진 합성 중첩 올리고들(올리고들은 일반적으로 짧은 DNA 서열이다)에 의해서 합성 플라스미드를 생성하여 전체 서열을 생성할 수 있다. 이 기술을 사용하면 세포-특이적 최적상태로 발현된 신규 서열을 생성할 수 있다. SEQ. ID. NOs. 9, 11 및 13을 가진 플라스미드 PGE는 각각 PI-PspI, I-CeuI 및 I-SceI에 의해서 소화되었고, 관심의 유전자, 예를 들어 Nif-H, Nif-D, Nif-K 및 aadA를 SEQ. ID. NO. 15를 가진 신규 플라스미드가 색소체에 사용되었던 경우 SEQ. ID. NO. 7를 가진 것과 동일한 부위에 기증했다.

다음에, SEQ. ID. NO. 2를 가진 PEG가 금 입자와 결합되고 토마토 식물의 잎 조직에 폭격되었다.

토마토 엽록체 형질전환은 토마토 식물에서 식물 색소체 DNA(trnfM 및 tmG)의 기지 부위들 간 상동성 재조합에 기초한 외래 DNA의 통합을 허용한다. 일반적으로, 각 사이드의 수백 염기쌍(BPs)은 그 사이에 수천의 새로운 BP를 전달하는 것을 가능하게 한다(Nif 유전자)(도 5 참조). 플라스미드 DNA의 사용은 관심의 이들 유전자를 운반하는데, 이들 유전자는 1) E. coli 같은 박테리아에 DNA를 유지하고; 2) CDS의 돌연변이발생을 촉진하고, 3) 플라스미드 DNA와 금 입자를 결합하고 그것을 토마토 식물의 잎 조직에 폭격한다. aadA 같은 분비 마커를 부가하고 스트렙토마이신 또는 스펙티노마이신 항생제를 함유하는 식물 배지에서 트랜스펙션된 식물을 선택하는 것은 "마커"를 함유하지 않은 세포를 죽이는 결과를 가져올 것이다. 수주 후에 생존한 세포는 트랜스제닉 색소체(엽록체) 호모색소체를 함유할 것이며, 이것은 모든 엽록체가 동일하고 신규 유전자를 함유한다는 것을 시사하는데, 이것은 간단한 부위-특이적 PCR 시험에 의해서 입증될 수 있다. 식물의 잎은 배지 중 호르몬의 농도에 따라서 새로운 식물로 발달할 것이다(재생).

트랜스제닉 식물은 Nif-H, Nif-D 및 Nif-K의 Nif 오페론, 또는 이들 세 가지 중 어느 두 개의 Nif 오페론을 발현할 것이다. 용어 "오페론"은 DNA 서열 순서로 조직되고 함께 전사된 몇몇 유전자를 말한다. 언급된 유전자는 RNA 분자로 전사되고 및/또는 단백질 또는 펩티드로 번역될 수 있는 DNA 서열 또는 DNA 서열의 일부이다.

잎에 플라즈미드 DNA의 폭격 후 이종성 재조합이 일어난다. 식물의 세포 생활 주기의 정상적 일부로서, 두 분자(플라스미드 DNA 및 색소체 DNA)는 재조합 부위들의 유사성에 따라서 식물의 DNA의 일부를 대체할 것이다. 결과적으로, 플라스미드 DNA의 가외 조각이 색소체 DNA에 도입될 것이다.

식물 조직이 항생제에 노출됨에 따라 항생체에 의한 손상을 피할 수 있는 색소체만이 생존할 것이며(항생제에 내성인), 궁극적으로 몇 세대 후에는 트랜스제닉 색소체만이 각 세포에 존재하게 될 것이다. 이 지점에서 관련된 식물, 세포 또는 조직은 호모플라즘으로 간주될 수 있다.

멸균 토마토 식물(L. esculentum var. IAC-Santa Clara)을 MS 배지에서 발아된 표면-멸균된 종자로부터 Magenta 상자(커넥터 요소를 가진 이중 상자)에서 길렀다. 바이오리스틱 폭격을 위해서 줄기 절단시 웃자란 곁 분열조직으로부터 생성된 3-4주령 식물(대략 15cm 높이)로부터 어린 잎을 수거했다. 호모플라즘 색소체형질전환 식물 및 야생형 대조군 식물을 토양으로 옮겨 피토챔버에서 성숙할 때까지 성장시켰다(16h 명, 8h 암, 24℃). 대조군 식물도 동일한 조건에서 성장되었다.

DuPont PDS1000He 바이오리스틱 건과 1,100psi 파열판(BioRad Laboratories, Hercules, CA)를 사용하여 어린 멸균 토마토 잎에 플라스미드 DNA-코팅된 6μm 직경 금 입자를 바이오리스틱 폭격에 의해서 토마토 식물의 색소체 형질전환을 달성했다. 폭격된 잎 샘플을 작은 조각(3x3mm)들로 잘라 스펙티노마이신(300-500mg/L)을 함유하는 RMOP 배지로 옮겨서 3-4개월 동안 약광(25μE; 16h 명, 8h 암) 아래서 인큐베이션했다. 스펙티노마이신에 내성인 주 계통은 황색 또는 연녹색 성장 캘러스로 확인되었다. 호모플라즘 색소체형질전환 조직의 추가의 증식과 분리를 위해 캘러스 조각을 동일한 배지로 옮겼다. 식물 재생을 위하여, 호모플라즘 캘러스 조직을 0.2mg/L IAA와 3mg/L BAP를 함유하는 아가-고화된 MS 배지의 표면 위로 옮겼다. 또는 달리, BAP 대신에 2mg/L 제아틴을 가진 동일한 배지에서 순을 유도했다. 뿌리를 내리기 위해서 재생된 순을 식물호르몬-무함유 MS 배지를 담은 상자로 옮겼다. RNOP 배지는 성장 호르몬이 지원된 MS이다: NAA 0.1mg/L, BAP 1mg/L, 및 비타민 티아민 1mg/L, 미오이노시톨 100mg/L 및 탄소원으로서 수크로오스 30g.

시아노박테리아 같은 미생물은 미생물적 다양성을 가지므로 본 발명에는 대안적인 유전자 또는 단백질이 사용될 수 있으며, 예를 들어 표 2에 제시된 유전자가 사용될 수 있다. 일부 유전자들은 동일하진 않지만 유사하며 거의 동일하게 기능하므로, Nif-D, Nif-H, Nif-K 및 CDS에 대한 상이한 공급원들이 사용될 수 있다.

도 5는 발아 3주 후 토마토 식물의 발생을 도시한다. 왼쪽 화분에는 식묘시에 단일 용량의 비료가 공급되었고, 오른쪽 화분에는 식물에 비료가 첨가되지 않았다. 이들은 정상 현장 조건에 함께 유지되었고, 오른쪽의 마커의 크기는 1 인치이다.

도 6은 식물 세포에서 Nif-H의 표적화 및 축적을 도시한다. 특히, (A) 렙토링브야 노둘로사(Leptolyngbya nodulosa)로부터의 비-헤테로시스트 시아노박테리아 Nif-H가 Nif-H-GFP의 합성을 위한 주형으로 사용되었다. 트랜짓 펩티드의 경우, 5' 단부에 토마토 루비스코 트랜짓 펩티드를 암호화하는 210 염기가 부가되었다. 대조군으로서 빈 벡터가 사용되었다. (B)는 공초점 현미경에 의해서 촬영된 엽록체 자발형광 및 GFP를 도시한다. 중첩(병합) 이미지는 TP-Nif-H-GFP가 잎 엽록체에서 축적될 수 있으며, 세포의 다른 부분 및 소기관에서는 검출될 수 없다는 것을 증명한다.

사용될 수 있는 다른 예는 바이오연료 또는 바이오디젤 생산을 위한 모델로서, 혹은 녹색 비료로서 사용될 수 있는 단세포 조류인 클라미도모나스 레인하드티(Chlamidomonas reinhardtii)이며, 이것은 색소체에서 발현을 허용하는 특정 요소, 즉 프로모터 및 번역 개시 서열뿐만 아니라 상동성 재조합 서열을 가진 시아노박테리아 SEQ ID NOs. 1-3(또는 다른 질소-고정 박테리아)로부터의 Nif-H 및 Nif-D 및 Nif-K의 유전자 서열을 함유하는 DNA로 유전적으로 형질전환된다.

결과의 유전자 변형된 C. reinhardtii는 니트로게나아제 리덕타아제 효소에 대한 유전자를 지니고 그것을 발현할 것이며, 질소 고정에 대한 박테리아 경로를 모방한다. 유전자 조작된 C. reinhardtii를 통해 식물에서 질소 고정에 BNF를 이용하기 위해서 그것은 식물 또는 동물 소비를 위해 이용가능한 유기 형태로 질소를 생성한다. 많은 조류가 상업적 제품을 위한 아주 미미한 조정하에 이 기술과 함께 사용될 수 있으며, 종래의 시비보다 비용이 15-35% 적게 드는 환경 친화적인 결과와 더 빠른 성장을 야기한다. 조류를 형질전환할 때 Nif 유전자의 공급원은 광합성 시아노박테리움이다. 이들 Nif 유전자를 사용하는 것은 형질전환된 조류가 그것의 대사에 이 효소를 이용할 수 있도록 보장한다.

본 개시는 하나의 오페론으로서 Nif 유전자의 발현을 허용하는 신규 플라스미드를 설명한다. 특유의 구성은 이전 실험의 복잡한 문제를 회피하며, 그 결과 개별적이며 분리된 유전자의 발현이 얻어진다.

Nif 유전자의 추가의 공급원 및 Nif 유전자의 분리는 박테리아 게놈 서열이 공개적으로 알려지는 대로 완료될 것이다. 특정 표적 유전자를 증폭함으로써 시아노박테리아의 Nif 유전자가 이용될 수 있다. 예를 들어, Nostoc sp. PCC 7120 승인 번호 BA000019.2(NCBI)의 게놈 서열에서 Nif-H는 유전자의 처음과 마지막 24 염기를 사용한 PCR에 의해 쉽게 증폭될 수 있으며, 이것은 ATGACTGACGAAAACATTAGACAG (SEQ. ID. NO. 3)에서 시작하여 ATGACTGACGAAAACATTAGACAGA(SEQ. ID. NO. 25)에서 끝난다. 각 프라이머의 앞머리에 제한 부위를 부가하는 것은 코딩 서열 내에서 발견되지 않는 특정 부위에서 PCR 생성물의 클로닝을 가능하게 하는데, 제1 프라이머: CTCGAG-ATGACTGACGAAAACATTAGACAG(SEQ. ID. NO. 26)에 대해 XhoI 부위, 및 제2 프라이머: TCTAGA-ATGACTGACGAAAACATTAGACAGA(SEQ. ID, NO. 27)에 대해 XbaI 부위를 예로서 들 수 있다. 이것은 Nif-H 유전자를 XhoI-XbaI 부위에 클로닝하는 것을 가능하게 한다. pBluescript와 같은 플라스미드에 900bp 크기의 PCR 생성물의 결찰과 이어서 암피실린-함유 LB 아가 평판 상에서의 선택은 서열의 검증을 허용한다. 동일한 전략이 Nif-D 및 Nif-K 유전자의 클로닝에도 적용될 것이다.

실시예: 색소체 형질전환

trnfM 및 tmG 같은 식물 색소체 DNA의 기지 부위들 간의 상동성 재조합에 기초한 외래 DNA의 통합을 허용하는 C. reinhardtii 엽록체 형질전환이 도 2-3에 도시되며, 이것은 SEQ ID. NOs. 1-3 중 하나를 사용한다.

일반적으로, 각 사이드의 수백 염기쌍이 그 사이에 수천 개의 신규 DNA 염기쌍(BP)을 전달할 수 있게 된다(Nif 유전자). 이들 유전자를 운반하는 플라스미드 DNA는 1) E. coli 같은 박테리아에 DNA를 유지하고, 2) 코딩 DNA 서열(CDS)의 서열 및/또는 돌연변이유발을 허용하고, 3) 유전적 형절진환에 이용될 수 있다.

유전적 형질전환(플라스미드를 색소체에 삽입, 도 7B 참조)은 다음의 단계들을 포함한다: 1) 금 입자 위에, 예를 들어 SEQ. ID. NO. 1, SEQ. ID. NO. 2 또는 SEQ. ID. NO. 3을 암호화하는 핵산 서열과 같은 DNA를 흡수하고, C. reinhardtii 세포 위에서 DNA를 폭격하는 단계; 2) 예를 들어 aadA와 같은 선택 마커를 부가하고, 예를 들어 스펙티노마이신과 같은 항생제를 함유하는 아가 성장 배지 상에서 형질전환된 세포를 선택함으로써 트랜스젠을 함유하지 않는 세포를 제거하는 단계; 3) 트랜스제닉 색소체를 함유하는 세포를 저장하는 단계; 및 4) 간단한 부위-특이적 PCR을 사용하여 서열을 검중하는 단계.

일단 변형되면 C. reinhardtii는, 예를 들어 아세틸렌 환원에 의한 질소 고정에 대해 시험될 것이다. 이 과정은 가스 크로마토그래피를 사용하여 질소 공급원인 아세틸렌의 양을 측정하며, 이로써 Nif 효소 활성의 직접적인 증거를 제공할 수 있다. 형질전환된 박테리아를 성장시켜서 그것을 야생형과 비교하는 것은 형질전환된 유전자가 활성이며, 이로써 C. reinhardtii가 질소 고정을 허용한다는 것을 입증한다.

실시예: 핵 형질전환(도 7A 참조)

담배 식물 제조: 담배 식물은 장일 성장 챔버에서 22℃에서 MS 배지 상에서 Majecta 상자에서 발아 후 3-4주 동안 성장된다.

아그로박테리움 배양물 제조: 아그로박테리움 배양물은 3mL LB + 항생제(예를 들어, 리팜핀* 10mg/L과 스펙티노마이신 100mg/mL) + 플라스미드 Seq # PGE0048 또는 PGE0066 또는 PGE0088 또는 PGE0148에서 하룻밤 종자 배양물을 성장시킴으로써 제조된다(28℃, 250rpm, 신선하게 다시 스트리킹된 LB 평판). 1 또는 2mL를 항생제 없이 50mL LB에 희석하고 OD600 = 0.6 내지 1.0까지 28℃에서 2-6시간 동안 성장시킨다.

형질전환 과정: 박테리아 배양물을 멸균 페트리 디쉬에 붓는다. 담배 식물의 잎을, 예를 들어 원판이나 사각형과 같은 작은 조각으로, 일반적으로 약 2cm x 2cm 조각으로 자른다.

담배 식물의 조각을 페트리 디쉬의 박테리아 배양물에 첨가한다. 페트리 디쉬를 22-25℃에서 약 20분 동안 인큐베이션한다. 담배 식물의 조각을 멸균 여과지 상에서 건조시켜 어떤 과잉의 액체를 제거한다. 담배 식물의 건조된 조각을 MR 평판 위에 둔다(가능하다면 축을 향한 쪽을 아래로). 평판을 파라필름으로 밀봉한다.

또는 달리, 담배 식물의 하나 이상의 잎에 OD 0.1의 박테리아 현탁액이 주사될 수 있다. 다음에, 주사된 잎을 48-72시간 후 회수한다. 주사된 잎을 Nif 유전자의 일시 발현에 대해 분석한다. 48-72시간 후, 식물을 96시간 동안 15N 고정 동위원소와 함께 챔버에 넣고, 이후 질량분광법에 의해서 식물 아미노산 및/또는 다른 분자로의 15N의 통합에 대해 분석했다.

동시-재배: 22℃에서 성장 챔버에서 3일 동안 잎/조각을 인큐베이션한다(장일). 3일 후, 잎/조각을 Timentin 100mg/L 수용액으로 멸균수에서 헹궈서 세척했다. 잎/조각을 MRTK 위에 두었으며, 이때 아그로박테리움 재성장의 위험을 감소시키기 위해서 잎/조각을 서로 이격시켰다. 잎/조각을 22℃에서 약 2-3주 동안 성장 챔버에 둔다(장일). 잎/조각의 가장자리에 캘러스가 나타날 것이다. 캘러스 상에 작은 싹들이 나타났을 때 이들을 MSTK 평판에 다시 평판하는데, 이때 동시에 묘목에 손상 없이 주변 캘러스와 잎 조직을 제거하는 것이 바람직하다. 약 3주 후(때로 더 이상), 작은 식물이 발생하여 뿌리가 나타날 것이며, 이후 작은 식물은 MST 상에 다시 평판될 수 있다(Majenta 상자에서, 재평판 전에 뿌리를 절단한다). 다음에, 3-4주마다 MS Majenta 상자에 재평판한다.

배지 조성

MR = MS 배지(1L), MS 분말 4.4g, 수크로오스 30g, MES 0.5g pH 5.8 아가 8.0g. 고압멸균 후, BAP 1mL 스톡 용액(1mg/mL)과 NAA 0.1mL 스톡 용액(1mg/mL) 첨가.

MRTK = MR과 동일하지만 고압멸균 후, 티멘틴 300mg/L 최종(필터 멸균 스톡 용액 300mg/mL로부터)과 카나마이신 50mg/L 최종(필터 멸균 스톡 용액 50mg/mL로부터) 첨가.

MSTK = MRTK와 동일하지만 BAP와 NAA가 없고 티멘틴 300mg/L 최종과 카나마이신 30mg/L 최종 첨가.

MST = MSTK와 동일하지만 카나마이신이 없고, 티멘틴은 100mg/L까지 감소될 수 있으며, 추가의 재평판에서는 완전히 제거될 수 있다.

Sequence ID No. 1:

토마토 색소체 형질전환 벡터 PGE 11, 완전한 서열

플라스미드 DNA 1..12166

토마토, 시아노박테리아 기타 서열; 인공 서열; 벡터.

정의 토마토 색소체 형질전환 및 클로닝 벡터 PGE11 완전 서열.

승인 PGE 0011

핵심어 토마토 색소체 형질전환; 식물 Nif 발현 영역;

공급원 식물 유전자 조작 11

유기체 Escherichia coli Xl1B

저자 Zaltsman Adi

제목 Nif-H 및 Nif-D-K 클러스터, 변형된 플라스미드 벡터의 토마토 안정한 형질전환 및 발현을 위한 플라스미드

특징 Location/Qualifiers

CDS 보체(11181..12038)

AMP^R (BLA) product = "beta-lactamase"

/protein_id="AAA66380.1

rep_origin 보체(10404..11018) ORI-PUC

misc_feature 보체(8470..9959)

Tom\Chl\2- 상동성 재조합을 위한 토마토 색소체 서열

misc_feature 659..2582

Tom\CHL\1- 상동성 재조합을 위한 토마토 색소체 서열

CDS 보체(8660..8730) tRNA-G

CDS 2495..2568 tRNA-fM

터미네이터 8205..8399 PsbA\trminator

프로모터 2644..2836 rrn16P

CDS 6926..7855 aadA Spc/Strep^R

CDS 5316..6851 인공 NifK

CDS 3806..5296 인공 인공 NifD

CDS 2837..3721 인공 NifH

1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct

61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg

121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact

181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac

241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga

301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga

361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca

421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg

481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg

541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg

601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg

661 attggggcgt ggacataagg gtctttatga cacaatcaac aattcgcttc attttcaatt

721 aggccttgct ctagcttctt taggggttat tacttctttg gtagctcaac acatgtactc

781 tttacctgct tatgcattca tagcacaaga ctttactact caagctgcat tatataccca

841 ccaccaatat atcgcaggat tcatcatgac aggagctttt gctcatggag ctatattttt

901 cattagagat tacaatccgg agcaaaatga agataatgta ttggcaagaa tgttagatca

961 taaagaagct atcatatctc atttaagttg ggccagcctc tttctgggat tccataccct

1021 gggactttat gttcataatg atgtcatgct tgcctttggc actccggaga agcaaatctt

1081 gattgaaccg atatttgctc aatggataca atccgctcat ggtaaaactt catatgggtt

1141 cgatgtactt ttatcttcaa cgactggccc agcattcaat gcgggtcgaa gcatctggtt

1201 gccgggttgg ttaaatgctg ttaatgaaaa tagtaattca ttattcttaa caataggtcc

1261 tggagacttt ttggttcatc atgctattgc ccttggttta catacaacta cattgatctt

1321 agtaaaaggt gctttagatg cacgtggttc caagttaatg ccagataaaa aggatttcgg

1381 ttatagtttt ccgtgcgatg gcccaggacg aggcggtact tgtgatattt cggcatggga

1441 cgcgttttat ttggcagttt tttggatgtt aaatactatt ggatgggtta ctttttattg

1501 gcattggaag cacatcacat tatggcaagg taacgtttca cagtttaatg aatcttccac

1561 ttatttgatg ggctggttaa gggattattt atggttaaac tcttcacaac ttatcaatgg

1621 atataaccct tttggtatga atagtttatc ggtttgggca tggatgttct tatttggaca

1681 tcttgtttgg gctactggat ttatgttctt aatttcttgg cgtggatatt ggcaggaatt

1741 gattgaaact ttagcatggg ctcacgaacg cacacctttg gccaatttga ttcgatggag

1801 agataaacca gtggcccttt ctattgtaca agcaagattg gttggattag ctcacttttc

1861 tgtaggttat atattcactt atgcggcttt cttgattgcc tctacgtcgg gcaaatttgg

1921 ttaattaatg tgtgtattcg cgataatctc atttctttcg acggagaagg gggtccacct

1981 tcttctattt ctacatctag gattcgactt gtatcatgga tactaatagg aattcaacca

2041 ttatggcaag gaaaagtttg attcagaggg agaagaagag gcaaaaattg gaacagaaat

2101 atcattcgat tcgtcgatcc tcaaaaaaag aaataagcaa ggttccgtcg ttgagtgaca

2161 aatgggaaat ttatggaaag ttacaatccc taccacggaa tagtgcacct acacgccttc

2221 atcgacgttg ttttttgacc ggaaggccga gagctaacta tcgagacttt ggcctatccg

2281 gacacatact tcgtgaaatg gttcatgcat gtttgttgcc aggagcaaca agatcaagtt

2341 ggtaaggatt aacgcttcat ttctatttct atggtcgatg atcatagaag cccctttacc

2401 attctgtata aatgggctat tctatttgta cagatagggt ggaggggcgc atttaatcct

2461 tgtttatcta ttagttttca gttcttatct ttggcgcggg gtagagcagt ttggtagctc

2521 gcaaggctca taaccttgag gtcacgggtt caaatcctgt ctccgcaaca tcttattctg

2581 gtctcgaggt ccgcataaag aaccacccat aatacccata atagctgttt gccaaccggt

2641 cgccgtcgtt caatgagaat ggataagagg ctcgtgggat tgacgtgagg gggcagggat

2701 gactatattt ctgggagcga actccgggcg aatatgaagc gcatggatac aagttatgcc

2761 ttggaatgaa agacaattcc gaatccgctt tgtctacgaa caaggaagct ataagtaatg

2821 caactatgaa ggatctatga ctgatgaaaa tattagacaa attgcttttt atggaaaagg

2881 aggaattgga aaatctacta cttctcaaaa tactttagct gctatggctg aaatgggaca

2941 aagaattatg attgtaggat gtgatcctaa agctgattct actagattaa tgttacattc

3001 taaagctcaa actactgtat tacatttagc tgctgaaaga ggagctgtag aagatttaga

3061 attacatgaa gtaatgttaa ctggatttag aggagtaaaa tgtgtagaat ctggaggacc

3121 tgaacctgga gtaggatgtg ctggaagagg aattattact gctattaatt ttttagaaga

3181 aaatggagct tatcaagatt tagattttgt atcttatgat gtattaggag atgtagtatg

3241 tggaggattt gctatgccta ttagagaagg aaaagctcaa gaaatttata ttgtaacttc

3301 tggagaaatg atggctatgt atgctgctaa taatattgct agaggaattt taaaatatgc

3361 tcattctgga ggagtaagat taggaggatt aatttgtaat tctagaaaag tagatagaga

3421 agatgaatta attatgaatt tagctgaaag attaaatact caaatgattc attttgtacc

3481 tagagataat attgtacaac atgctgaatt aagaagaatg actgtaaatg aatatgctcc

3541 tgattctaat caaggacaag aatatagagc tttagctaaa aaaattatta ataatgataa

3601 attaactatt cctactccta tggaaatgga tgaattagaa gctttattaa ttgaatatgg

3661 attattagat gatgatacta aacattctga aattattgga aaacctgctg aagctactaa

3721 atagcggccg caacccataa tacccataat agctgtttgc catcgctacc ttaggaccgt

3781 tatagttaac cggtggaggc agactatgac tcctcctgaa aataaaaatt tagtagatga

3841 aaataaagaa ttaattcaag aagtattaaa agcttatcct gaaaaatcta gaaaaaaaag

3901 agaaaaacat ttaaatgtac atgaagaaaa taaatctgat tgtggagtaa aatctaatat

3961 taaatctgta cctggagtaa tgactgctag aggatgtgct tatgctggat ctaaaggagt

4021 agtatgggga cctattaaag atatgattca tatttctcat ggacctgtag gatgtggata

4081 ttggtcttgg tctggaagaa gaaattatta tgtaggagta actggaatta attcttttgg

4141 aactatgcat tttacttctg attttcaaga aagagatatt gtatttggag gagataaaaa

4201 attaactaaa ttaattgaag aattagatgt attatttcct ttaaatagag gagtatctat

4261 tcaatctgaa tgtcctattg gattaattgg agatgatatt gaagctgtag ctaaaaaaac

4321 ttctaaacaa attggaaaac ctgtagtacc tttaagatgt gaaggattta gaggagtatc

4381 tcaatcttta ggacatcata ttgctaatga tgctattaga gattggattt ttcctgaata

4441 tgataaatta aaaaaagaaa atagattaga ttttgaacct tctccttatg atgtagcttt

4501 aattggagat tataatattg gaggagatgc ttgggcttct agaatgttat tagaagaaat

4561 gggattaaga gtagtagctc aatggtctgg agatggaact ttaaatgaat taattcaagg

4621 acctgctgct aaattagtat taattcattg ttatagatct atgaattata tttgtagatc

4681 tttagaagaa caatatggaa tgccttggat ggaatttaat ttttttggac ctactaaaat

4741 tgctgcttct ttaagagaaa ttgctgctaa atttgattct aaaattcaag aaaatgctga

4801 aaaagtaatt gctaaatata ctcctgtaat gaatgctgta ttagataaat atagacctag

4861 attagaagga aatactgtaa tgttatatgt aggaggatta agacctagac atgtagtacc

4921 tgcttttgaa gatttaggaa ttaaagtagt aggaactgga tatgaatttg ctcataatga

4981 tgattataaa agaactactc attatattga taatgctact attatttatg atgatgtaac

5041 tgcttatgaa tttgaagaat ttgtaaaagc taaaaaacct gatttaattg cttctggaat

5101 taaagaaaaa tatgtatttc aaaaaatggg attacctttt agacaaatgc attcttggga

5161 ttattctgga ccttatcatg gatatgatgg atttgctatt tttgctagag atatggattt

5221 agctttaaat tctcctactt ggtctttaat tggagctcct tggaaaaaag ctgctgctaa

5281 agctaaagct gctgcttaac tcgagagata caacaatgcc tcaaaatcct gaaagaactg

5341 tagatcatgt agatttattt aaacaacctg aatatactga attatttgaa aataaaagaa

5401 aaaattttga aggagctcat cctcctgaag aagtagaaag agtatctgaa tggactaaat

5461 cttgggatta tagagaaaaa aattttgcta gagaagcttt aactgtaaat cctgctaaag

5521 gatgtcaacc tgtaggagct atgtttgctg ctttaggatt tgaaggaact ttaccttttg

5581 tacaaggatc tcaaggatgt gtagcttatt ttagaactca tttatctaga cattataaag

5641 aaccttgttc tgctgtatct tcttctatga ctgaagatgc tgctgtattt ggaggattaa

5701 ataatatgat tgaaggaatg caagtatctt atcaattata taaacctaaa atgattgctg

5761 tatgtactac ttgtatggct gaagtaattg gagatgattt aggagctttt attactaatt

5821 ctaaaaatgc tggatctatt cctcaagatt ttcctgtacc ttttgctcat actccttctt

5881 ttgtaggatc tcatattact ggatatgata atatgatgaa aggaatttta tctaatttaa

5941 ctgaaggaaa aaaaaaagct acttctaatg gaaaaattaa ttttattcct ggatttgata

6001 cttatgtagg aaataataga gaattaaaaa gaatgatggg agtaatggga gtagattata

6061 ctattttatc tgattcttct gattattttg attctcctaa tatgggagaa tatgaaatgt

6121 atcctggagg aactaaatta gaagatgctg ctgattctat taatgctaaa gctactgtag

6181 ctttacaagc ttatactact cctaaaacta gagaatatat taaaactcaa tggaaacaag

6241 aaactcaagt attaagacct tttggagtaa aaggaactga tgaattttta actgctgtat

6301 ctgaattaac tggaaaagct attcctgaag aattagaaat tgaaagagga agattagtag

6361 atgctattac tgattcttat gcttggattc atggaaaaaa atttgctatt tatggagatc

6421 ctgatttaat tatttctatt acttcttttt tattagaaat gggagctgaa cctgtacata

6481 ttttatgtaa taatggagat gatactttta aaaaagaaat ggaagctatt ttagctgctt

6541 ctccttttgg aaaagaagct aaagtatgga ttcaaaaaga tttatggcat tttagatctt

6601 tattatttac tgaacctgta gattttttta ttggaaattc ttatggaaaa tatttatgga

6661 gagatacttc tattcctatg gtaagaattg gatatccttt atttgataga catcatttac

6721 atagatattc tactttagga tatcaaggag gattaaatat tttaaattgg gtagtaaata

6781 ctttattaga tgaaatggat agatctacta atattactgg aaaaactgat atttcttttg

6841 atttaattag ataggcggcc gctcgctacc ttaggaccgt tatagttatt accctgttat

6901 ccctaaccgg tggaggcttc ttgttatgac atgttttttt ggggtacagt ctatgcctcg

6961 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa

7021 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt

7081 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga

7141 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca

7201 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc

7261 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc

7321 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg

7381 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc

7441 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt

7501 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc

7561 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa

7621 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa

7681 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact

7741 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca

7801 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatc

7861 tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccacctgatc

7921 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca

7981 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat

8041 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat

8101 aaaatttcta attcctaaaa ccaaaatcca gtgacgcggc cgcattaccc tgttatccct

8161 attaattaag agctcgctac cttaagagag gatatcggcg cgccgatcct agcctagtct

8221 ataggaggtt ttgaaaagaa aggagcaata atcattttct tgttctgtca agagggtgct

8281 attgctcctt tctttttttt tttttcttta ctaatttcct agtattttac tgacatagac

8341 ttttttgttt acattatcga aaaagaaaga gagggtattt gcttgcattt attcatgatg

8401 gatcccccgg gctgcaggca tgcaagctaa ttcccgatct agtaacatag atgacaccgc

8461 gcgccgcgga actagtaatt aattcccgcc tttcgctttt tgggggtgga aggcaaaaga

8521 aaacgtaggg gagggataga atcactacac tatcacggcc aactatacca actccttaat

8581 gtaaggatat atttaatgct atttatgaaa ttcaataata aaaagaaata gtaaaaaaat

8641 tactttatct tggatcttgg gcggatagcg ggaatcgaac ccgcatcttc tccttggcaa

8701 agagaaattt taccattcga ccatatccgc atttttttgt tcttgataca caatatgtac

8761 ccacatatat gatatataac cggatcttat ttgtgcagtg ccgggacaca tattctcttc

8821 ggaacgattc caataatttt gttaattata ttctttttat tcaagaagtt tgacccccct

8881 ctaatttttt tgttttcttt atttgatttg cattttcttt ggggacttag attcaaattt

8941 aatgtgtctc acaaccgaga aaaattaggg gggtcatttt ggttttgggt ctgcgacgaa

9001 taggttcaag agatgagaga attaaggata cccaccagaa agactaatcc aatccataag

9061 gaggtaccag aaaatacaac atttttgtta cttgaccagc catcaggaga agcaaataca

9121 acgggtacgc taatcaataa gattaatgaa gtagcaatta atgcaaaaac agccaattgg

9181 aaagcaagag tcatgctttt aatcctccaa gctaccaaca aatgaactat ataccatttg

9241 atccctctat cagccaaaaa atattaattg tgataaaata tgtcatcgag ggattttact

9301 ttatcatgaa tccattgatt ctatatgact tattactact ccccctttcg cactttattc

9361 gtacatggag tggggtgggg ggaaatggaa ttttcttttt tatttcacaa atggacatgc

9421 tagatcatat atctatatac ggatagatag atcgatcggc ggattcgcac ctgagatctt

9481 tctacagata gtgggggtat ccacccctat agccatgttc tattcggagg aataaaataa

9541 aaatagtctt tcggagagat ggctgagtgg ttgatagccc cggtcttgaa aaccggtata

9601 gttttgaaca aagaactatc gagggttcga atccctctct ctcctttttt gctaattgaa

9661 tagatttttt tatttagtgg ttttgcccaa tctgctatcc gaaagaaaag ggaatggctc

9721 ggctatccca cctagccaag ccagaaaaat agattagata taaattagat aaaataaatg

9781 agttgaaaaa aaaaaaagaa aaaaggaata cttaagctga ttccaagatg tatgattgaa

9841 tcaaagtaat ttgtacttca ttcaagcatt ggatctcctg tctcatatca attaagaggg

9901 gtcatggaaa gaacaggttc aaagtcgcga tcaattcctt tttcaaatcc tgctgcagcg

9961 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca

10021 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga

10081 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg

10141 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc

10201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac

10261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata

10321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa

10381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct

10441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa

10501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg

10561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca

10621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa

10681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg

10741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg

10801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg

10861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc

10921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag

10981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac

11041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc

11101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag

11161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt

11221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag

11281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca

11341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact

11401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca

11461 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg

11521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc

11581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg

11641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca

11701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt

11761 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc

11821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc

11881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca

11941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa

12001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat

12061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa

12121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac

Sequence ID No. 2:

토마토 색소체 형질전환 벡터 PGE 0011T, 완전한 서열

플라스미드 DNA 1..12166 인공 최적화 CDS.

토마토, 시아노박테리아 기타 서열; 인공 서열; 벡터.

정의 토마토 색소체 형질전환 및 클로닝 벡터 PGE11 완전 서열

승인 PGE 0011T

핵심어 토마토 색소체 형질전환; 식물 Nif 발현 영역;

공급원 식물 유전자 조작 0011T

유기체 Escherichia coli Xl1B

REFERENCE 1(염기 1 내지 2979)

저자 Zaltsman Adi

제목 Nif-H 및 Nif-D-K 클러스터, 변형된 플라스미드 벡터의 토마토 안정한 형질전환 및 발현을 위한 플라스미드

공급원 1..12166

유기체 = 시아노박테리아의 Nif H, D, K 유전자 및 aadA 및 인공 서열을 가진 원래 클로닝 벡터 pBluescript

CDS Coli BLA보체(11181..12038)

rep_origin ORIE1보체(10404..11018)

misc_feature 보체(8470..9959)

Tom\Chl\2 misc_feature 659..2582

/label=Tom\CHL\1

CDS TRNA-G보체(8660..8730)

CDS tRNA-fM 2495..2568

터미네이터 PsbA\trminator 8205..8399

프로모터rrn16P 2644..2836

CDS aadA 6926..7855

CDS NifK 5316..6851

CDS NifD 3806..5296

CDS NifH 2837..3721

BASE COUNT 3643 a 2207 c 2651 g 3665 t

1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct

61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg

121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact

181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac

241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga

301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga

361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca

421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg

481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg

541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg

601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg

661 attggggcgt ggacataagg gtctttatga cacaatcaac aattcgcttc attttcaatt

721 aggccttgct ctagcttctt taggggttat tacttctttg gtagctcaac acatgtactc

781 tttacctgct tatgcattca tagcacaaga ctttactact caagctgcat tatataccca

841 ccaccaatat atcgcaggat tcatcatgac aggagctttt gctcatggag ctatattttt

901 cattagagat tacaatccgg agcaaaatga agataatgta ttggcaagaa tgttagatca

961 taaagaagct atcatatctc atttaagttg ggccagcctc tttctgggat tccataccct

1021 gggactttat gttcataatg atgtcatgct tgcctttggc actccggaga agcaaatctt

1081 gattgaaccg atatttgctc aatggataca atccgctcat ggtaaaactt catatgggtt

1141 cgatgtactt ttatcttcaa cgactggccc agcattcaat gcgggtcgaa gcatctggtt

1201 gccgggttgg ttaaatgctg ttaatgaaaa tagtaattca ttattcttaa caataggtcc

1261 tggagacttt ttggttcatc atgctattgc ccttggttta catacaacta cattgatctt

1321 agtaaaaggt gctttagatg cacgtggttc caagttaatg ccagataaaa aggatttcgg

1381 ttatagtttt ccgtgcgatg gcccaggacg aggcggtact tgtgatattt cggcatggga

1441 cgcgttttat ttggcagttt tttggatgtt aaatactatt ggatgggtta ctttttattg

1501 gcattggaag cacatcacat tatggcaagg taacgtttca cagtttaatg aatcttccac

1561 ttatttgatg ggctggttaa gggattattt atggttaaac tcttcacaac ttatcaatgg

1621 atataaccct tttggtatga atagtttatc ggtttgggca tggatgttct tatttggaca

1681 tcttgtttgg gctactggat ttatgttctt aatttcttgg cgtggatatt ggcaggaatt

1741 gattgaaact ttagcatggg ctcacgaacg cacacctttg gccaatttga ttcgatggag

1801 agataaacca gtggcccttt ctattgtaca agcaagattg gttggattag ctcacttttc

1861 tgtaggttat atattcactt atgcggcttt cttgattgcc tctacgtcgg gcaaatttgg

1921 ttaattaatg tgtgtattcg cgataatctc atttctttcg acggagaagg gggtccacct

1981 tcttctattt ctacatctag gattcgactt gtatcatgga tactaatagg aattcaacca

2041 ttatggcaag gaaaagtttg attcagaggg agaagaagag gcaaaaattg gaacagaaat

2101 atcattcgat tcgtcgatcc tcaaaaaaag aaataagcaa ggttccgtcg ttgagtgaca

2161 aatgggaaat ttatggaaag ttacaatccc taccacggaa tagtgcacct acacgccttc

2221 atcgacgttg ttttttgacc ggaaggccga gagctaacta tcgagacttt ggcctatccg

2281 gacacatact tcgtgaaatg gttcatgcat gtttgttgcc aggagcaaca agatcaagtt

2341 ggtaaggatt aacgcttcat ttctatttct atggtcgatg atcatagaag cccctttacc

2401 attctgtata aatgggctat tctatttgta cagatagggt ggaggggcgc atttaatcct

2461 tgtttatcta ttagttttca gttcttatct ttggcgcggg gtagagcagt ttggtagctc

2521 gcaaggctca taaccttgag gtcacgggtt caaatcctgt ctccgcaaca tcttattctg

2581 gtctcgaggt ccgcataaag aaccacccat aatacccata atagctgttt gccaaccggt

2641 cgccgtcgtt caatgagaat ggataagagg ctcgtgggat tgacgtgagg gggcagggat

2701 gactatattt ctgggagcga actccgggcg aatatgaagc gcatggatac aagttatgcc

2761 ttggaatgaa agacaattcc gaatccgctt tgtctacgaa caaggaagct ataagtaatg

2821 caactatgaa ggatctatga ctgatgaaaa tattagacaa attgcttttt atggaaaagg

2881 aggaattgga aaatctacta cttctcaaaa tactttagct gctatggctg aaatgggaca

2941 aagaattatg attgtaggat gtgatcctaa agctgattct actagattaa tgttacattc

3001 taaagctcaa actactgtat tacatttagc tgctgaaaga ggagctgtag aagatttaga

3061 attacatgaa gtaatgttaa ctggatttag aggagtaaaa tgtgtagaat ctggaggacc

3121 tgaacctgga gtaggatgtg ctggaagagg aattattact gctattaatt ttttagaaga

3181 aaatggagct tatcaagatt tagattttgt atcttatgat gtattaggag atgtagtatg

3241 tggaggattt gctatgccta ttagagaagg aaaagctcaa gaaatttata ttgtaacttc

3301 tggagaaatg atggctatgt atgctgctaa taatattgct agaggaattt taaaatatgc

3361 tcattctgga ggagtaagat taggaggatt aatttgtaat tctagaaaag tagatagaga

3421 agatgaatta attatgaatt tagctgaaag attaaatact caaatgattc attttgtacc

3481 tagagataat attgtacaac atgctgaatt aagaagaatg actgtaaatg aatatgctcc

3541 tgattctaat caaggacaag aatatagagc tttagctaaa aaaattatta ataatgataa

3601 attaactatt cctactccta tggaaatgga tgaattagaa gctttattaa ttgaatatgg

3661 attattagat gatgatacta aacattctga aattattgga aaacctgctg aagctactaa

3721 atagcggccg caacccataa tacccataat agctgtttgc catcgctacc ttaggaccgt

3781 tatagttaac cggtggaggc agactatgac tcctcctgaa aataaaaatt tagtagatga

3841 aaataaagaa ttaattcaag aagtattaaa agcttatcct gaaaaatcta gaaaaaaaag

3901 agaaaaacat ttaaatgtac atgaagaaaa taaatctgat tgtggagtaa aatctaatat

3961 taaatctgta cctggagtaa tgactgctag aggatgtgct tatgctggat ctaaaggagt

4021 agtatgggga cctattaaag atatgattca tatttctcat ggacctgtag gatgtggata

4081 ttggtcttgg tctggaagaa gaaattatta tgtaggagta actggaatta attcttttgg

4141 aactatgcat tttacttctg attttcaaga aagagatatt gtatttggag gagataaaaa

4201 attaactaaa ttaattgaag aattagatgt attatttcct ttaaatagag gagtatctat

4261 tcaatctgaa tgtcctattg gattaattgg agatgatatt gaagctgtag ctaaaaaaac

4321 ttctaaacaa attggaaaac ctgtagtacc tttaagatgt gaaggattta gaggagtatc

4381 tcaatcttta ggacatcata ttgctaatga tgctattaga gattggattt ttcctgaata

4441 tgataaatta aaaaaagaaa atagattaga ttttgaacct tctccttatg atgtagcttt

4501 aattggagat tataatattg gaggagatgc ttgggcttct agaatgttat tagaagaaat

4561 gggattaaga gtagtagctc aatggtctgg agatggaact ttaaatgaat taattcaagg

4621 acctgctgct aaattagtat taattcattg ttatagatct atgaattata tttgtagatc

4681 tttagaagaa caatatggaa tgccttggat ggaatttaat ttttttggac ctactaaaat

4741 tgctgcttct ttaagagaaa ttgctgctaa atttgattct aaaattcaag aaaatgctga

4801 aaaagtaatt gctaaatata ctcctgtaat gaatgctgta ttagataaat atagacctag

4861 attagaagga aatactgtaa tgttatatgt aggaggatta agacctagac atgtagtacc

4921 tgcttttgaa gatttaggaa ttaaagtagt aggaactgga tatgaatttg ctcataatga

4981 tgattataaa agaactactc attatattga taatgctact attatttatg atgatgtaac

5041 tgcttatgaa tttgaagaat ttgtaaaagc taaaaaacct gatttaattg cttctggaat

5101 taaagaaaaa tatgtatttc aaaaaatggg attacctttt agacaaatgc attcttggga

5161 ttattctgga ccttatcatg gatatgatgg atttgctatt tttgctagag atatggattt

5221 agctttaaat tctcctactt ggtctttaat tggagctcct tggaaaaaag ctgctgctaa

5281 agctaaagct gctgcttaac tcgagagata caacaatgcc tcaaaatcct gaaagaactg

5341 tagatcatgt agatttattt aaacaacctg aatatactga attatttgaa aataaaagaa

5401 aaaattttga aggagctcat cctcctgaag aagtagaaag agtatctgaa tggactaaat

5461 cttgggatta tagagaaaaa aattttgcta gagaagcttt aactgtaaat cctgctaaag

5521 gatgtcaacc tgtaggagct atgtttgctg ctttaggatt tgaaggaact ttaccttttg

5581 tacaaggatc tcaaggatgt gtagcttatt ttagaactca tttatctaga cattataaag

5641 aaccttgttc tgctgtatct tcttctatga ctgaagatgc tgctgtattt ggaggattaa

5701 ataatatgat tgaaggaatg caagtatctt atcaattata taaacctaaa atgattgctg

5761 tatgtactac ttgtatggct gaagtaattg gagatgattt aggagctttt attactaatt

5821 ctaaaaatgc tggatctatt cctcaagatt ttcctgtacc ttttgctcat actccttctt

5881 ttgtaggatc tcatattact ggatatgata atatgatgaa aggaatttta tctaatttaa

5941 ctgaaggaaa aaaaaaagct acttctaatg gaaaaattaa ttttattcct ggatttgata

6001 cttatgtagg aaataataga gaattaaaaa gaatgatggg agtaatggga gtagattata

6061 ctattttatc tgattcttct gattattttg attctcctaa tatgggagaa tatgaaatgt

6121 atcctggagg aactaaatta gaagatgctg ctgattctat taatgctaaa gctactgtag

6181 ctttacaagc ttatactact cctaaaacta gagaatatat taaaactcaa tggaaacaag

6241 aaactcaagt attaagacct tttggagtaa aaggaactga tgaattttta actgctgtat

6301 ctgaattaac tggaaaagct attcctgaag aattagaaat tgaaagagga agattagtag

6361 atgctattac tgattcttat gcttggattc atggaaaaaa atttgctatt tatggagatc

6421 ctgatttaat tatttctatt acttcttttt tattagaaat gggagctgaa cctgtacata

6481 ttttatgtaa taatggagat gatactttta aaaaagaaat ggaagctatt ttagctgctt

6541 ctccttttgg aaaagaagct aaagtatgga ttcaaaaaga tttatggcat tttagatctt

6601 tattatttac tgaacctgta gattttttta ttggaaattc ttatggaaaa tatttatgga

6661 gagatacttc tattcctatg gtaagaattg gatatccttt atttgataga catcatttac

6721 atagatattc tactttagga tatcaaggag gattaaatat tttaaattgg gtagtaaata

6781 ctttattaga tgaaatggat agatctacta atattactgg aaaaactgat atttcttttg

6841 atttaattag ataggcggcc gctcgctacc ttaggaccgt tatagttatt accctgttat

6901 ccctaaccgg tggaggcttc ttgttatgac atgttttttt ggggtacagt ctatgcctcg

6961 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa

7021 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt

7081 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga

7141 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca

7201 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc

7261 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc

7321 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg

7381 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc

7441 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt

7501 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc

7561 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa

7621 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa

7681 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact

7741 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca

7801 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatc

7861 tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccacctgatc

7921 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca

7981 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat

8041 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat

8101 aaaatttcta attcctaaaa ccaaaatcca gtgacgcggc cgcattaccc tgttatccct

8161 attaattaag agctcgctac cttaagagag gatatcggcg cgccgatcct agcctagtct

8221 ataggaggtt ttgaaaagaa aggagcaata atcattttct tgttctgtca agagggtgct

8281 attgctcctt tctttttttt tttttcttta ctaatttcct agtattttac tgacatagac

8341 ttttttgttt acattatcga aaaagaaaga gagggtattt gcttgcattt attcatgatg

8401 gatcccccgg gctgcaggca tgcaagctaa ttcccgatct agtaacatag atgacaccgc

8461 gcgccgcgga actagtaatt aattcccgcc tttcgctttt tgggggtgga aggcaaaaga

8521 aaacgtaggg gagggataga atcactacac tatcacggcc aactatacca actccttaat

8581 gtaaggatat atttaatgct atttatgaaa ttcaataata aaaagaaata gtaaaaaaat

8641 tactttatct tggatcttgg gcggatagcg ggaatcgaac ccgcatcttc tccttggcaa

8701 agagaaattt taccattcga ccatatccgc atttttttgt tcttgataca caatatgtac

8761 ccacatatat gatatataac cggatcttat ttgtgcagtg ccgggacaca tattctcttc

8821 ggaacgattc caataatttt gttaattata ttctttttat tcaagaagtt tgacccccct

8881 ctaatttttt tgttttcttt atttgatttg cattttcttt ggggacttag attcaaattt

8941 aatgtgtctc acaaccgaga aaaattaggg gggtcatttt ggttttgggt ctgcgacgaa

9001 taggttcaag agatgagaga attaaggata cccaccagaa agactaatcc aatccataag

9061 gaggtaccag aaaatacaac atttttgtta cttgaccagc catcaggaga agcaaataca

9121 acgggtacgc taatcaataa gattaatgaa gtagcaatta atgcaaaaac agccaattgg

9181 aaagcaagag tcatgctttt aatcctccaa gctaccaaca aatgaactat ataccatttg

9241 atccctctat cagccaaaaa atattaattg tgataaaata tgtcatcgag ggattttact

9301 ttatcatgaa tccattgatt ctatatgact tattactact ccccctttcg cactttattc

9361 gtacatggag tggggtgggg ggaaatggaa ttttcttttt tatttcacaa atggacatgc

9421 tagatcatat atctatatac ggatagatag atcgatcggc ggattcgcac ctgagatctt

9481 tctacagata gtgggggtat ccacccctat agccatgttc tattcggagg aataaaataa

9541 aaatagtctt tcggagagat ggctgagtgg ttgatagccc cggtcttgaa aaccggtata

9601 gttttgaaca aagaactatc gagggttcga atccctctct ctcctttttt gctaattgaa

9661 tagatttttt tatttagtgg ttttgcccaa tctgctatcc gaaagaaaag ggaatggctc

9721 ggctatccca cctagccaag ccagaaaaat agattagata taaattagat aaaataaatg

9781 agttgaaaaa aaaaaaagaa aaaaggaata cttaagctga ttccaagatg tatgattgaa

9841 tcaaagtaat ttgtacttca ttcaagcatt ggatctcctg tctcatatca attaagaggg

9901 gtcatggaaa gaacaggttc aaagtcgcga tcaattcctt tttcaaatcc tgctgcagcg

9961 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca

10021 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga

10081 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg

10141 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc

10201 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac

10261 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata

10321 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa

10381 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct

10441 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa

10501 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg

10561 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca

10621 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa

10681 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg

10741 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg

10801 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg

10861 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc

10921 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag

10981 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac

11041 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc

11101 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag

11161 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt

11221 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag

11281 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca

11341 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact

11401 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca

11461 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg

11521 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc

11581 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg

11641 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca

11701 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt

11761 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc

11821 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc

11881 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca

11941 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa

12001 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat

12061 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa

12121 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac

Sequence ID No. 3:

클라미도모나스 색소체 형질전환 벡터 PGE 11, 완전한 서열

PGE0011c Synt Chlamydomonas 11875bp DNA 원형

플라스미드 DNA 1..11875

클라미도모나스, 시아노박테리아 기타 서열; 인공 서열; 벡터

정의 클라미도모나스 색소체 형질전화 및 클로닝 벡터 PGE0011c 완전 서열

승인 PGE 0011c

버전 N/A

핵심어 클로미도모나스 색소체 형질전환; 조류 Nif 발현

공급원 식물 유전자 조작 11c

유기체 Escherichia coli Xl1B

저자 Zaltsman Adi

제목 Nif-H 및 Nif-D-K 클러스터, 변형된 플라스미드 벡터의 클라미도모나스 안정한 형질전환 및 발현을 위한 플라스미드

CDS Ecoli BLA보체(10890..11747)

rep_origin ORIE1보체(10113..10727)

CDS aadA 인공 6696..7625

CDS NifD 인공 3576..5066

CDSNifHartificia 2607..3491

CDS NifK 인공 5086..6621

misc_featureChloroplast 659..1998

misc_featureChloroplast 8219..9668

프로모터 rrn16s 2060..2606

터미네이터 psbA 7975..8148

BASE COUNT 3742 a 2024 c 2352 g 3757 t

기원

1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct

61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg

121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact

181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac

241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga

301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga

361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca

421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg

481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg

541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg

601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccat

661 atttaatatg gcatcaccag aggtaccaga aatgtattta cctttcctcc caaaaacact

721 tattttaaaa tctaattatt gacagaagtt gaccttgtaa atattactta aattaattta

781 gcagtatgtc aatttagtta gttatagtac cactttggga gagggtatta cattaattta

841 ttttgaaatt tacttatagt aaatagagat ttgggaacaa caactgctag cctaccgatt

901 tacaaaacgt ggttttaata atttattaat tggaaggcga ctgcaaaaga atattttgca

961 gcataggatt tatttctaat ttttctaaag ttaccaattg agttaaaaaa agctttctta

1021 attcaacatt tttaagtaaa tactgtttaa tgttatactt ttacgaatac acatatggta

1081 aaaaataaaa caatatcttt aaaataagta aaaataattt gtaaaccaat aaaaaatata

1141 tttatggtat aatataacat atgatgtaaa aaaaactatt tgtctaattt aataaccatg

1201 cattttttat gaacacataa taattaaaag cgttgctaat ggtgtaaata atgtatttat

1261 taaattaaat aattgttatt ataaggagaa atccatgaca attagtactc cagagcgcga

1321 agcgaaaaaa gtaaagattg cggttgatcg taatcctgta gaaacaagtt ttgaaaaatg

1381 ggcgtgcgac ctgaaataag gatttgacta attaaatata aaaattttta actaaaataa

1441 gctgaaaaag cttattttag ttttaagcac tgttggccga gcggatgagg caaacgactc

1501 ataatcgtta taaggtaggt tcaactccta tacggtgcaa ctaagtaaag aataagcaat

1561 acttcctttt caaaatatgg atggtcataa tatatgctat tctggtagaa tttataacaa

1621 gccttttcag aagatatata tatataactt tttatagtaa acttgttgga gatttaaatt

1681 aacatctagg agcaatcgtc cactctaatg agttaatata actctaaaag ctgaaagtaa

1741 tccatattgt tcctactccg gttttctaag ttatttttgt taaaggagtc gtctggttta

1801 ctccaacaaa agaaattggt tttatttttt ctatgtcata tacatgcgat agagtattag

1861 tttttaatac ttgcttaaaa aaaatttttg tgatataata ttattagatt aagttaatgc

1921 ggacatagct caatggtaga gtatttcctt gccaaggaaa atgttgcggg ttcgactccc

1981 gttgtccgct agaaactact cgaggtccgc ataaagaacc acccataata cccataatag

2041 ctgtttgcca accggtcgcg cctgccaact gcctatattt atatactgcg ataaacttta

2101 gtcccgaagg ggtttacata tccgaaggag gaagcaggca gtggcggtac cacgccactg

2161 gcgtcctaat ataaatattg ggcaagtaaa cttagaataa aatttatttg ctgcgttagc

2221 aggtttacat actcctaagt ttacttgccc gaaggggaag gaggacgtcc cctacgggaa

2281 tataaatatt agtggcagtg gtacaataaa taaattgtat gtaaacccct tcgggcaact

2341 aaagtttatc gcagtattaa catcctagta tataaatatc ggcagttggc aggcaacaaa

2401 tttatttatt gtcccgtaag gggaagggga aaacaattat tattttactg cggagcagct

2461 tgttattaga aatttttatt aaaaaaaaaa taaaaatttg acaaaaaaaa ataaaaaagt

2521 taaattaaaa acactgggaa tgttctaaca atcataaaaa aatcaaaagg gtttaaaatc

2581 ccgacaaaat ttaaacttta aagagtatga cagatgaaaa tattcgtcaa attgcttttt

2641 atggtaaagg tggtattggt aaatcaacaa catcacaaaa tacattagct gctatggctg

2701 aaatgggtca acgtattatg attgttggtt gtgatccaaa agctgattca acacgtttaa

2761 tgttacattc aaaagctcaa acaacagttt tacatttagc tgctgaacgt ggtgctgttg

2821 aagatttaga attacatgaa gttatgttaa caggttttcg tggtgttaaa tgtgttgaat

2881 caggtggtcc agaaccaggt gttggttgtg ctggtcgtgg tattattaca gctattaatt

2941 ttttagaaga aaatggtgct tatcaagatt tagattttgt ttcatatgat gttttaggtg

3001 atgttgtttg tggtggtttt gctatgccaa ttcgtgaagg taaagctcaa gaaatttata

3061 ttgttacatc aggtgaaatg atggctatgt atgctgctaa taatattgct cgtggtattt

3121 taaaatatgc tcattcaggt ggtgttcgtt taggtggttt aatttgtaat tcacgtaaag

3181 ttgatcgtga agatgaatta attatgaatt tagctgaacg tttaaataca caaatgattc

3241 attttgttcc acgtgataat attgttcaac atgctgaatt acgtcgtatg acagttaatg

3301 aatatgctcc agattcaaat caaggtcaag aatatcgtgc tttagctaaa aaaattatta

3361 ataatgataa attaacaatt ccaacaccaa tggaaatgga tgaattagaa gctttattaa

3421 ttgaatatgg tttattagat gatgatacaa aacattcaga aattattggt aaaccagctg

3481 aagctacaaa atagcggccg caacccataa tacccataat agctgtttgc catcgctacc

3541 ttaggaccgt tatagttaac cggtggaggc agactatgac accaccagaa aataaaaatt

3601 tagttgatga aaataaagaa ttaattcaag aagttttaaa agcttatcca gaaaaatcac

3661 gtaaaaaacg tgaaaaacat ttaaatgttc atgaagaaaa taaatcagat tgtggtgtta

3721 aatcaaatat taaatcagtt ccaggtgtta tgacagctcg tggttgtgct tatgctggtt

3781 caaaaggtgt tgtttggggt ccaattaaag atatgattca tatttcacat ggtccagttg

3841 gttgtggtta ttggtcatgg tcaggtcgtc gtaattatta tgttggtgtt acaggtatta

3901 attcatttgg tacaatgcat tttacatcag attttcaaga acgtgatatt gtttttggtg

3961 gtgataaaaa attaacaaaa ttaattgaag aattagatgt tttatttcca ttaaatcgtg

4021 gtgtttcaat tcaatcagaa tgtccaattg gtttaattgg tgatgatatt gaagctgttg

4081 ctaaaaaaac atcaaaacaa attggtaaac cagttgttcc attacgttgt gaaggttttc

4141 gtggtgtttc acaatcatta ggtcatcata ttgctaatga tgctattcgt gattggattt

4201 ttccagaata tgataaatta aaaaaagaaa atcgtttaga ttttgaacca tcaccatatg

4261 atgttgcttt aattggtgat tataatattg gtggtgatgc ttgggcttca cgtatgttat

4321 tagaagaaat gggtttacgt gttgttgctc aatggtcagg tgatggtaca ttaaatgaat

4381 taattcaagg tccagctgct aaattagttt taattcattg ttatcgttca atgaattata

4441 tttgtcgttc attagaagaa caatatggta tgccatggat ggaatttaat ttttttggtc

4501 caacaaaaat tgctgcttca ttacgtgaaa ttgctgctaa atttgattca aaaattcaag

4561 aaaatgctga aaaagttatt gctaaatata caccagttat gaatgctgtt ttagataaat

4621 atcgtccacg tttagaaggt aatacagtta tgttatatgt tggtggttta cgtccacgtc

4681 atgttgttcc agcttttgaa gatttaggta ttaaagttgt tggtacaggt tatgaatttg

4741 ctcataatga tgattataaa cgtacaacac attatattga taatgctaca attatttatg

4801 atgatgttac agcttatgaa tttgaagaat ttgttaaagc taaaaaacca gatttaattg

4861 cttcaggtat taaagaaaaa tatgtttttc aaaaaatggg tttaccattt cgtcaaatgc

4921 attcatggga ttattcaggt ccatatcatg gttatgatgg ttttgctatt tttgctcgtg

4981 atatggattt agctttaaat tcaccaacat ggtcattaat tggtgctcca tggaaaaaag

5041 ctgctgctaa agctaaagct gctgcttaac tcgagagata caacaatgcc acaaaatcca

5101 gaacgtacag ttgatcatgt tgatttattt aaacaaccag aatatacaga attatttgaa

5161 aataaacgta aaaattttga aggtgctcat ccaccagaag aagttgaacg tgtttcagaa

5221 tggacaaaat catgggatta tcgtgaaaaa aattttgctc gtgaagcttt aacagttaat

5281 ccagctaaag gttgtcaacc agttggtgct atgtttgctg ctttaggttt tgaaggtaca

5341 ttaccatttg ttcaaggttc acaaggttgt gttgcttatt ttcgtacaca tttatcacgt

5401 cattataaag aaccatgttc agctgtttca tcatcaatga cagaagatgc tgctgttttt

5461 ggtggtttaa ataatatgat tgaaggtatg caagtttcat atcaattata taaaccaaaa

5521 atgattgctg tttgtacaac atgtatggct gaagttattg gtgatgattt aggtgctttt

5581 attacaaatt caaaaaatgc tggttcaatt ccacaagatt ttccagttcc atttgctcat

5641 acaccatcat ttgttggttc acatattaca ggttatgata atatgatgaa aggtatttta

5701 tcaaatttaa cagaaggtaa aaaaaaagct acatcaaatg gtaaaattaa ttttattcca

5761 ggttttgata catatgttgg taataatcgt gaattaaaac gtatgatggg tgttatgggt

5821 gttgattata caattttatc agattcatca gattattttg attcaccaaa tatgggtgaa

5881 tatgaaatgt atccaggtgg tacaaaatta gaagatgctg ctgattcaat taatgctaaa

5941 gctacagttg ctttacaagc ttatacaaca ccaaaaacac gtgaatatat taaaacacaa

6001 tggaaacaag aaacacaagt tttacgtcca tttggtgtta aaggtacaga tgaattttta

6061 acagctgttt cagaattaac aggtaaagct attccagaag aattagaaat tgaacgtggt

6121 cgtttagttg atgctattac agattcatat gcttggattc atggtaaaaa atttgctatt

6181 tatggtgatc cagatttaat tatttcaatt acatcatttt tattagaaat gggtgctgaa

6241 ccagttcata ttttatgtaa taatggtgat gatacattta aaaaagaaat ggaagctatt

6301 ttagctgctt caccatttgg taaagaagct aaagtttgga ttcaaaaaga tttatggcat

6361 tttcgttcat tattatttac agaaccagtt gattttttta ttggtaattc atatggtaaa

6421 tatttatggc gtgatacatc aattccaatg gttcgtattg gttatccatt atttgatcgt

6481 catcatttac atcgttattc aacattaggt tatcaaggtg gtttaaatat tttaaattgg

6541 gttgttaata cattattaga tgaaatggat cgttcaacaa atattacagg taaaacagat

6601 atttcatttg atttaattcg ttaggcggcc gctcgctacc ttaggaccgt tatagttatt

6661 accctgttat ccctaaccgg tggaggcttc ttgttatgac atgttttttt ggtgttcaat

6721 caatgccacg tgcttcaaaa caacaagctc gttatgctgt tggtcgttgt ttaatgttat

6781 ggtcatcaaa tgatgttaca caacaaggtt cacgtccaaa aacaaaatta aatattatgg

6841 gtgaagctgt tattgctgaa gtttcaacac aattatcaga agttgttggt gttattgaac

6901 gtcatttaga accaacatta ttagctgttc atttatatgg ttcagctgtt gatggtggtt

6961 taaaaccaca ttcagatatt gatttattag ttacagttac agttcgttta gatgaaacaa

7021 cacgtcgtgc tttaattaat gatttattag aaacatcagc ttcaccaggt gaatcagaaa

7081 ttttacgtgc tgttgaagtt acaattgttg ttcatgatga tattattcca tggcgttatc

7141 cagctaaacg tgaattacaa tttggtgaat ggcaacgtaa tgatatttta gctggtattt

7201 ttgaaccagc tacaattgat attgatttag ctattttatt aacaaaagct cgtgaacatt

7261 cagttgcttt agttggtcca gctgctgaag aattatttga tccagttcca gaacaagatt

7321 tatttgaagc tttaaatgaa acattaacat tatggaattc accaccagat tgggctggtg

7381 atgaacgtaa tgttgtttta acattatcac gtatttggta ttcagctgtt acaggtaaaa

7441 ttgctccaaa agatgttgct gctgattggg ctatggaacg tttaccagct caatatcaac

7501 cagttatttt agaagctcgt caagcttatt taggtcaaga agaagatcgt ttagcttcac

7561 gtgctgatca attagaagaa tttgttcatt atgttaaagg tgaaattaca aaagttgttg

7621 gtaaataatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat

7681 ccacctgatc tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta

7741 tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg

7801 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac

7861 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacgcggc cgcattaccc

7921 tgttatccct attaattaag agctcgctac cttaagagag gatatcggcg cgcctttttt

7981 tttaaactaa aataaatctg gttaaccata cctggtttat tttagtttat acacactttt

8041 catatatata tacttaatag ctaccatagg cagttggcag gacgtcccct tacgggacaa

8101 atgtatttat tgttgcctgc caactgccta atataaatat tagtggacgg atcccccggg

8161 ctgcaggcat gcaagctaat tcccgatcta gtaacataga tgacaccgcg cgccgcggaa

8221 gaatagaagt tacaacgtaa ttctacgttg ggttaattta ttaacccctt aaaggggttg

8281 catttagaat ccaggcatct tgggtaaatt atgtataata aaactatatt aaaagttaat

8341 tcattaaagc cgtttattta aaataaataa aaaattatgt cacgttattt aggccctcgt

8401 ttgagagtta ttcgtcgtat tggtaaatta agaggtttta cgcgtaaaaa acctttccgt

8461 cgtgtattta aaggttttgg tggttttaaa ggtaaagtta ttcctccagg tcaacatggt

8521 ttaacaaaac tattaaaaac aagaccatat gattcatctg aatcggatta tctaatccgt

8581 ttaaaagtaa aacaacgttt acgttttaac tatggtatta ctgaacgtca acttgttaac

8641 tatgtacgta aagctaaaaa aattaaagaa tctacaggtc aagttttact acaattttta

8701 gaaatgcgtt tagataatat tgtattccgt ttaaatatgg cacctacaat tccagcagca

8761 cgccaactta ttagtcatgg acatattcgt gtaaataata aaaaagtaaa tattcctagc

8821 tatatgtgta aaccaaaaga tgttatttct gtagcaatga aacaacgttc gttacaactt

8881 gtaaataaaa acttacaaga atattaccgt cgtatgcgtt tctataaaaa acgtttagaa

8941 aaaacattac cttttatttt actaaaaatt aaaccattag gtcttactag tgtaacagca

9001 gctgtagaac ttattactaa aggaaacgtg cgagtaaata ataagagtgt gaaaacgcca

9061 aattatattt gtcgtccacg agatacagtt tctttaagaa caaaacaagg tattaaaaaa

9121 gtatttttaa aaaattattt aaaaggttaa tttaatcggt aaaattccca ggtagttgca

9181 cctttccctt caggacgtcc ccttcccctt cggacaataa ataaatttgt tgcctgccaa

9241 caaatttatt tattgtatta aaatagaata aaatttattt gctgcggtag caggtttaca

9301 tacaatttat ttattgtacc actccactgg cgtccttcgg agtatgtaaa catgcttggc

9361 actggtttac atacaattta tttattgtac cactgccact gcgtcctcct tcggagtatg

9421 taaacatgct aagtttactt gcccaatatt tatattaggc agttggcagg caactcacta

9481 aaatttattt acccgaagga cgtcccgaag ggaaggggaa ggaggacgtc cccttacggg

9541 aatataaata ttagtggacg tcagtggcgg taccactgcc actggcgtcc tatatttata

9601 tactcctaag tttacttgcc caatatttat atacccgaag gaaggggaag catataaata

9661 tacttcggga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa

9721 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata

9781 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta

9841 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa

9901 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg

9961 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag

10021 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa

10081 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc

10141 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca

10201 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg

10261 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct

10321 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt

10381 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag

10441 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc

10501 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac

10561 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga

10621 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc

10681 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg

10741 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca

10801 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt

10861 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca

10921 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg

10981 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca

11041 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt

11101 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt

11161 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca

11221 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca

11281 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga

11341 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact

11401 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga

11461 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg

11521 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc

11581 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga

11641 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat

11701 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt

11761 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt

11821 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac

Sequence ID No. 4: rrn16의 프로모터 서열

4A> 16S 리보솜 rrn16에 대한 클라미도모나스 레인하드티 엽록체

Gcctgccaactgcctatatttatatactgcgataaactttagtcccgaaggggtttacatatccgaaggaggaagcaggcagtggcggtaccacgccactggcgtcctaatataaatattgggcaagtaaacttagaataaaatttatttgctgcgttagcaggtttacatactcctaagtttacttgcccgaaggggaaggaggacgtcccctacgggaatataaatattagtggcagtggtacaataaataaattgtatgtaaaccccttcgggcaactaaagtttatcgcagtattaacatcctagtatataaatatcggcagttggcaggcaacaaatttatttattgtcccgtaaggggaaggggaaaacaattattattttactgcggagcagcttgttattagaaatttttattaaaaaaaaaataaaaatttgacaaaaaaaaataaaaaagttaaattaaaaacactgggaatgttctaacaatcataaaaaaatcaaaagggtttaaaatcccgacaaaatttaaactttaaagagt

4B> 16S 리보솜 rrn16에 대한 토마토 솔라눔 리코페르시쿰 엽록체

cgtcgttcaatgagaatggataagaggctcgtgggattgacgtgagggggcagggatgactatatttctgggagcgaactccgggcgaatatgaagcgcatggatacaagttatgccttggaatgaaagacaattccgaatccgctttgtctacgaacaaggaagctataagtaatgcaactatgaaggatct

[표 1]

[표 2]

[표 3] SEQ. ID. NO. 46

일시적 및 안정한 형질전환을 위한 식물 뉴클레아제 형질전환 플라스미드

NptII TP Nif HDK42 PGE # 0088

특징 Location/Qualifiers

CDS 12469..13437 spectinomycin^R

repeat_region 13889..14169 ORI-E1

rep_origin 보체(14979..15979) PVS1-rep

misc_feature 1730..1797 /label=RB

misc_feature 보체(12089..12132) LB

CDS 보체(2544..3335) Reporter NptII

인공 CDS 4661..5848 TP-NifH42

인공 CDS 9951..11690 TP-NifK42

인공 CDS 7041..8795 TP-NifD42

프로모터 3771..4660 2X35SP

프로모터 6151..7037 2X35SP

프로모터 9057..9947 2X35SP

1 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga

61 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc

121 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg

181 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc

241 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg

301 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga

361 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt

421 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat

481 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag

541 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc

601 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa

661 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc

721 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc

781 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg

841 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc

901 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg

961 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc

1021 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg

1081 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg

1141 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg

1201 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca

1261 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc

1321 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg

1381 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc

1441 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag

1501 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca

1561 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc

1621 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg

1681 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta

1741 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga

1801 caatctgatc caagctcaag ctaagcttca gagccaccac cctcagagcc gccaccagaa

1861 ccaccaccag agccgccgcc agcattgaca ggaggcccga tctagtaaca tagatgacac

1921 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat

1981 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat

2041 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc

2101 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggatcat

2161 ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtccat

2221 ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccgg gcccgatcat

2281 attgtcgctc aggatcgtgg cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc

2341 accttcgacc gcctgttccg cagagatccc gtgggcgaag aactccagca tgagatcccc

2401 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata

2461 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat

2521 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc

2581 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca

2641 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc

2701 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag

2761 caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg

2821 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca

2881 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat

2941 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact

3001 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc

3061 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc

3121 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg

3181 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca

3241 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc

3301 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccaga tccggtgcag

3361 attatttgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa

3421 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt

3481 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg

3541 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc

3601 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcagattg

3661 tcgtttcccg ccttcaggta ccgcgatcgc tcgcgacctg caggcataaa gccgtcagtg

3721 tccgcataaa gaaccaaccc ataataccca taatagctgt ttgccaaccg gtcaacatgt

3781 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag

3841 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc

3901 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca

3961 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga

4021 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa

4081 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat

4141 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc

4201 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga

4261 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga

4321 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa

4381 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt

4441 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc

4501 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct

4561 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa

4621 gcaattttct gaaaattttc accatttacg aacgatagcc atggctagcg ctaccggact

4681 cagatctatg gcttcctctg tcatttcttc agcagctgtt gccacacgca gcaatgttac

4741 acaagctagc atggttgcac ctttcactgg tctcaaatct tcagccactt tccctgttac

4801 aaagaagcaa aaccttgaca tcacttccat tgctagcaat ggtggaagag ttagctgcag

4861 agctcaaatg ggccgcaaca gccagggctt cctgacgacg atccgcatca tcgtcacgag

4921 cgcgacggac cagccgagca acttcatcca cagcctgagc aacaagcgcg agagcacgat

4981 gcgccagatc gcgttctacg gcaagggcgg catcggcaag agcacgacga gccagaacac

5041 gatcgcggcg ctggcggaga cgaaccgcat catgatcgtc ggctgcgacc cgaaggcgga

5101 cagcacgcgc ctgatgctgc acacgaaggc gcagacgacg atcctgcacc tggcggcgga

5161 gcgcggcacg gtcgaggaca tcgagctgga ggaggtcctg ctggagggct accagggcgt

5221 caagtgcgtc gagagcggcg gcccggagcc gggcgtcggc tgcgcgggcc gcggcatcat

5281 cacggcgatc aacttcctgg aggaggaggg cgcgtacgag gacctggact tcgtcagcta

5341 cgacgtcctg ggcgacgtcg tctgcggcgg cttcgcgatg ccgatccgcg agggcaaggc

5401 gcaggagatc tacatcgtca cgagcggcga gatgatggcg atgtacgcgg cgaacaacat

5461 cgcgcgcggc atcctgaagt acgcgcacac gggcggcgtc cgcctgggcg gcctgatctg

5521 caacagccgc aacgtcaact gcgaggcgga gctgatcgag gagctggcgc gccgcctggg

5581 cacgcagatg atccacttcg tcccgcgcag caagcaggtc caggaggcgg agctgcgccg

5641 catgacggtc atcgagtaca gcccggacca cccgcaggcg caggagtacc gcgagctgag

5701 ccgcaagatc gagaacaaca cgaacctggt catcccgacg ccgatcacga tggaggagct

5761 ggaggagctg ctggtcgact tcggcatcct gggcggcgag gacgagtacg agaaggcgct

5821 gcaggcggac aaggcggcga cgaaggcgta gtaggatcca cctgatctag agtccgcaaa

5881 aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga

5941 gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat

6001 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt

6061 cctaaaacca aaatccagtg acgcggccgc acccataata cccataatag ctgtttgcca

6121 tcgctacctt aggaccgtta tagttaaccg gtcaacatgt ggagcacgac acacttgtct

6181 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac

6241 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg

6301 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg

6361 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga

6421 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata

6481 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag

6541 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc

6601 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca

6661 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc

6721 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt

6781 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc

6841 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacgt

6901 cgagagttct caacacaaca tatacaaaac aaacgaatct caagcaatca agcattctac

6961 ttctattgca gcaatttaaa tcatttcttt taaagcaaaa gcaattttct gaaaattttc

7021 accatttacg aacgatagcc atggctagcg ctaccggact cagatctatg gcttcctctg

7081 tcatttcttc agcagctgtt gccacacgca gcaatgttac acaagctagc atggttgcac

7141 ctttcactgg tctcaaatct tcagccactt tccctgttac aaagaagcaa aaccttgaca

7201 tcacttccat tgctagcaat ggtggaagag ttagctgcag agctcaaatg gttgcacctt

7261 tcactggtct caaatcttca gccactttcc ctgttacaaa gaagcaaaac cttgacatca

7321 cttccattgc tagcaatggt ggaagagtta gctggatggc tactgtagaa gataataaaa

7381 aattaattgc tgatgtatta tctacttatc ctgaaaaagc tgctaaaaaa agagctaaac

7441 atttaggagt atatgaagaa ggagaagctg attgtggagt aaaatctaat aaacaatctt

7501 tacctggagt aatgactgct agaggatgtg cttatgctgg atctaaagga gtagtatggg

7561 gacctattaa agatatggta catatttctc atggacctgt aggatgtgga tattattctt

7621 ggtctggaag aagaaattat tatattggaa ctactggagt agattctttt ggaactatgc

7681 aatttacttc tgattttcaa gaaagagata ttgtatttgg aggagataaa aaattagcta

7741 aaattattga tgaaattgaa gaattatttc ctttaaatgg aggagtatct gtacaatctg

7801 aatgtcctgt aggattaatt ggagatgata ttgaatctgt agctagaact aaatctaaag

7861 aaactggaaa atctgtagta cctgtaagat gtgaaggatt tagaggagta tctcaatctt

7921 taggacatca tattgctaat gatatgatta gagattgggt atttcctact gctgataaag

7981 aaaatgctga aaaaggattt gaaggaactc cttatgatgt agctattatt ggagattata

8041 atattggagg agatgcttgg tcttctagaa ttttattaga agaaattgga ttaagagtag

8101 tagctcaatg gtctggagat ggaactttaa ctgaaatgaa agctactcct aatgtaaaat

8161 taaatttaat tcattgttat agatctatga attatatttc tagacatatg gaagaaaaat

8221 atggaattcc ttggttagaa tataattttt ttggaccttc taaaattgct gcttctttaa

8281 gagaaattgc ttctagattt gatgaaaaaa ttcaagctaa agctgaagaa gtaattgaaa

8341 aatatagaaa acaatctgaa gaaattattg ctaaatatag acctagatta gaaggaaaaa

8401 ctgtaatgat gatggtagga ggattaagac ctagacatgt agtacctgct tttaaagatt

8461 taggaatgga aattattgga actggatatg aatttgctca tggagatgat tataaaagaa

8521 ctactggata tgtagaagat gctactttaa tttatgatga tgtaactgga tatgaatttg

8581 aagaatttgt aaaagaatta aaacctgatt tagtagctgc tggaattaaa gaaaaatatg

8641 tatttcaaaa aatggcttta ccttttagac aaatgcattc ttgggattat tctggacctt

8701 atcatggata tgatggattt gctatttttg ctagagatat ggatttagct ttaaattctc

8761 ctacttgggg attaattgga actccttgga ataaataagt aggatccacc tgatctagag

8821 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata

8881 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt

8941 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat

9001 ttctaattcc taaaaccaaa atccagtgac gcggccgcat taccctgtta tccctaaccg

9061 gtcaacatgt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag

9121 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat

9181 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct

9241 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg

9301 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca

9361 cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact

9421 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa

9481 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga

9541 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca

9601 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca

9661 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct

9721 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat

9781 aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac

9841 aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt

9901 taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagcc atggctagcg

9961 ctaccggact cagatctatg gcttcctctg tcatttcttc agcagctgtt gccacacgca

10021 gcaatgttac acaagctagc atggttgcac ctttcactgg tctcaaatct tcagccactt

10081 tccctgttac aaagaagcaa aaccttgaca tcacttccat tgctagcaat ggtggaagag

10141 ttagctgcag agctcaaatg gctcaaaatg taaataatat taaagatcat gtagatttat

10201 ttcatcaacc tgaatatcaa gaattatttg aaaataaaaa acaatttgaa ggaatgccta

10261 ctgctgaaaa agtacaagaa gtagctgaat ggactaaatc ttgggaatat agagaaaaaa

10321 attttgctag agaagcttta actgtaaatc ctgctaaagc ttgtcaacct ttaggagctt

10381 tattagctgc tattggattt gaaggaactt taccttttgt acatggatct caaggatgtg

10441 tagcttattt tagaactcat ttaactagac attttaaaga acctgtatct gctgtatctt

10501 cttctatgac tgaaaatgct gctgtatttg gaggattaaa aaatatggta gatggattac

10561 aaaattctta tgctttatat aaacctaaaa tgattgctgt atgtactact tgtatggctg

10621 aagtaattgg agatgattta ggagcttttt taggaaatgc tagacaagat ggagtaattc

10681 ctgatgattt acctgtacct tttgctcata ctccttcttt tgtaggatct catattactg

10741 gatatgattc tatgatgaaa tctattttat ctactttaac tgaaggaaaa aaaaaagaaa

10801 ctactaatgg aaaaattaat tttattgctg gatttgaaac ttatattgga aatgtaagag

10861 ctattaaaaa tattatttct gcttttgatt tagaaggaac tttattatct gatactgaaa

10921 tgtatttaga ttctcctaat ttaggagaat ttaaaatgta tcatgaagga acttctttag

10981 aagatgctgc tgattctatt aatgctgaag ctactgtaac tttacaaact tatactactc

11041 ctaaaactag agaatatatt gaaaaaaaat ggggacaaaa aacttatact tatagacctt

11101 ggggagtaaa aggaactgat gaatttttaa tgggattatc tgaattaact ggaaaaccta

11161 ttcctaaaga atttgaaatt gctagaggaa gagctgtaga tgctatgact gatactcaag

11221 cttgggtaca tggaaaaaga gctgctgtat atggagatcc tgatttagta atgggattat

11281 tacaatttat gttagaaatg ggaattgaac ctgtacatgt attagtaaat aattctacta

11341 aagaatttga agaagaagct aaagctttat tagctgcttc tccttatgga caacaagcta

11401 ctgtatgggg aggaaaagat ttatggcata tgagatcttt attatttact gaacctgtag

11461 attttttagt aggaaattct tatgctaaat atttacaaag agatactaaa actcctttaa

11521 ttagaattgg atatcctatt tttgatagac atcatttaca tagatattct actattggat

11581 atgaaggagc tattaattta ttaaattgga ttgctaatgg attaatggat gaattagata

11641 gaaaaactga tactccttct gtaactgata tttcttttga tttagtaaga taggatccac

11701 ctgatctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt

11761 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc

11821 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct

11881 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cgcggccgca ttaccctgtt

11941 atccctatta attaagagct cgctacctta agagaggata tcggcgcgcc tctagaattt

12001 aaatggatcc tacgtactcg aggaattcaa ttcggcgtta attcagtaca ttaaaaacgt

12061 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc

12121 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg

12181 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct

12241 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg

12301 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa

12361 ttcgggcacg aacccagtgg acataagcct cgttcggttc gtaagctgta atgcaagtag

12421 cgtaactgcc gtcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg

12481 cagtggcggt tttcatggct tcttgttatg acatgttttt ttggggtaca gtctatgcct

12541 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc

12601 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg

12661 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc

12721 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca

12781 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga

12841 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc

12901 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag

12961 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca

13021 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc

13081 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag

13141 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga

13201 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg

13261 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata

13321 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat

13381 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa

13441 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcc ggcgttaact caagcgatta

13501 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat

13561 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat

13621 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc

13681 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct

13741 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg

13801 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca

13861 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc

13921 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga

13981 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac

14041 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga

14101 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag

14161 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg

14221 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag

14281 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc

14341 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc

14401 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct

14461 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct

14521 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt

14581 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct

14641 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt

14701 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg

14761 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc

14821 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga

14881 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag

14941 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga

15001 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta

15061 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat

15121 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg

15181 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg

15241 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg

15301 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc

15361 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc

15421 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg

15481 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc

15541 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct

15601 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg

15661 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg

15721 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac

15781 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga

15841 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga

15901 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg

15961 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc

16021 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga

16081 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg

16141 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc

16201 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg

16261 tttgcgaccg tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc

16321 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca

16381 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc

16441 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc

16501 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc

16561 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt

16621 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc

16681 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct

16741 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg

16801 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc

16861 cggccagcgc ctcggcaacc tcaccgccgt

[표 4]-SEQ. ID. NO. 47

일시적 및 안정한 형질전환을 위한 식물 뉴클레아제 형질전환 플라스미드

NptII TP Nif HDK101 PGE # 66

특징 Location/Qualifiers

CDS 12307..13275 SPC-AADA^R

repeat_region 13727..14007 ORI-E1

rep_origin 보체(14817..15817) PVS1\rep\AGRO

misc_feature 1730..1797 RB

misc_feature 보체(11927..11970) LB

CDS 보체(2544..3335) Reporter-NptII

인공 CDS 4661..5755 TPH101

인공 CDS 6946..8607 TPD101

인공 CDS 9785..11527 TPK101

프로모터 3771..4658 2X35S

프로모터 6052..6944 2X35S

프로모터 8891..9782 2X35SP

1 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga

61 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc

121 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg

181 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc

241 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg

301 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga

361 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt

421 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat

481 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag

541 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc

601 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa

661 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc

721 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc

781 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg

841 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc

901 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg

961 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc

1021 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg

1081 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg

1141 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg

1201 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca

1261 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc

1321 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg

1381 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc

1441 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag

1501 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca

1561 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc

1621 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg

1681 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta

1741 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga

1801 caatctgatc caagctcaag ctaagcttca gagccaccac cctcagagcc gccaccagaa

1861 ccaccaccag agccgccgcc agcattgaca ggaggcccga tctagtaaca tagatgacac

1921 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat

1981 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat

2041 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc

2101 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggatcat

2161 ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtccat

2221 ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccgg gcccgatcat

2281 attgtcgctc aggatcgtgg cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc

2341 accttcgacc gcctgttccg cagagatccc gtgggcgaag aactccagca tgagatcccc

2401 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata

2461 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat

2521 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc

2581 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca

2641 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc

2701 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag

2761 caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg

2821 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca

2881 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat

2941 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact

3001 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc

3061 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc

3121 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg

3181 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca

3241 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc

3301 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccaga tccggtgcag

3361 attatttgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa

3421 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt

3481 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg

3541 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc

3601 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcagattg

3661 tcgtttcccg ccttcaggta ccgcgatcgc tcgcgacctg caggcataaa gccgtcagtg

3721 tccgcataaa gaaccaaccc ataataccca taatagctgt ttgccaaccg gtcaacatgt

3781 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag

3841 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc

3901 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca

3961 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga

4021 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa

4081 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat

4141 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc

4201 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga

4261 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga

4321 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa

4381 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt

4441 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc

4501 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct

4561 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa

4621 gcaattttct gaaaattttc accatttacg aacgatagcc atggctagcg ctaccggact

4681 cagatctatg gcttcctctg tcatttcttc agcagctgtt gccacacgca gcaatgttac

4741 acaagctagc atggttgcac ctttcactgg tctcaaatct tcagccactt tccctgttac

4801 aaagaagcaa aaccttgaca tcacttccat tgctagcaat ggtggaagag ttagctgcag

4861 agctcaaatg agacaaattg ctttttatgg aaaaggagga attggaaaat ctactacttc

4921 tcaaaatact ttagctgcta tggctaatag acatggacaa agaattatga ttgtaggatg

4981 tgatcctaaa gctgattcta ctagattaat tttaaatgct aaagctcaaa ctactgtatt

5041 acatgtagct gctgaaagag gagctgtaga agatgtagaa ttagatgaag tattaaaacc

5101 tggatttgga ggaattaaat gtgtagaatc tggaggacct gaacctggag taggatgtgc

5161 tggaagagga attattactg ctattaattt tttagaagaa gaaggagctt atactgattt

5221 agattttgta tcttatgatg tattaggaga tgtagtatgt ggaggatttg ctatgcctat

5281 tagagaaaat aaagctcaag aaatttatat tgtatgttct ggagaaatga tggctatgta

5341 tgctgctaat aatattgcta gaggagtatt aaaatatgct catgctggag gagtaagatt

5401 aggaggatta atttgtaatt ctagaaaagt agatagagaa actgaattaa ttgaaaattt

5461 agctgctaga ttaaatactc aaatgattca ttttgtacct agagataatg tagtacaaag

5521 agctgaaatt agaagaatga ctgtagaaca atatgctcct gaagataatc aagctcaaga

5581 atatgatcaa ttagctcaaa aaattattaa taatgaaaaa ttaactattc ctactccttt

5641 agaaatggat gaattagaag aattattaat tgaatttgga ttattaggag atgaagaaga

5701 tagacaaaaa caaattgctg ctcaagatgc tgctattaaa gctactgctg ctaaataagg

5761 atccacctga tctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc

5821 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag

5881 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat

5941 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacgcg gccgcaccca

6001 taatacccat aatagctgtt tgccatcgct accttaggac cgttatagtt aaccggtcaa

6061 catgtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac

6121 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat

6181 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa

6241 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc

6301 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct

6361 tcaaagcaag tggattgatg tgataacatg gtggagcacg acacacttgt ctactccaaa

6421 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta

6481 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata

6541 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt

6601 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg

6661 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact

6721 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga

6781 agttcatttc atttggagag gacgtcgaga gttctcaaca caacatatac aaaacaaacg

6841 aatctcaagc aatcaagcat tctacttcta ttgcagcaat ttaaatcatt tcttttaaag

6901 caaaagcaat tttctgaaaa ttttcaccat ttacgaacga tagccatggc tagcgctacc

6961 ggactcagat ctatggcttc ctctgtcatt tcttcagcag ctgttgccac acgcagcaat

7021 gttacacaag ctagcatggt tgcacctttc actggtctca aatcttcagc cactttccct

7081 gttacaaaga agcaaaacct tgacatcact tccattgcta gcaatggtgg aagagttagc

7141 tgcagagctc aaatggcttc tgaaaaaatt gaacaaaata aacaattaat tcaagaagta

7201 ttagatgctt atcctgctaa agctgctaaa agaagaaaaa aacatttaaa tgtaattgaa

7261 gaaaaaggag ctgattgtgg agtaaaatct aatgtaaaat ctgtacctgg agtaatgact

7321 actagaggat gtgcttttgc tggagctaaa ggagtagtat ggggacctgt aaaagatatg

7381 gtacatattt ctcatggacc tgtaggatgt ggatattatt cttgggctgg aagaagaaat

7441 tattataatg gagtaactgg agtagatact tttggaacta tgcaatttac ttctgatttt

7501 caagaaagag atattgtatt tggaggagat aaaaaattag ctaaaattat gaatgaaatt

7561 gaagaattat ttcctttaaa tgctggaatt actattgaat ctgaatgtcc tgtaggatta

7621 attggagatg atattgaagc tgtagctaaa aaagcttcta aagaattaaa taaacctgta

7681 gtacctgtaa gatgtgaagg atttagagga gtatctcaat ctttaggaca tcatattgct

7741 aatgatactg taagagattg ggtatatgaa ccttctgcta aagtaactaa tgaagaaatt

7801 ggatttgaaa aaactcctta tgatgtatct ttaattgctg attataatat tggaggagat

7861 ggatggtctt ctagattatt attagatgaa attggattaa gagtagtatc tcaagctact

7921 ggagatggaa cttataatga agtatttatg gctcctagag taaatttaaa tttaattcat

7981 tgttatagat ctatgaatta tatttgtaga tatatggaag aagaatatgg aattccttgg

8041 gtagaattta atttttttgg accttctcaa attgctaaat ctttaagaaa aattgcttct

8101 ttttttgatg ataaaattaa agaaaatact gaaaaagtaa ttgctagata tcaagaacaa

8161 gctgatgctg taattgctaa atatagacct agattagaag gaaaaaaagt aatgatgatg

8221 gtaggaggat taagacctag acatattatt cctgcttttg atgatttagg aatggaagta

8281 attggaactg gatatgaatt tggacatgga gatgattata aaagaactgc tgattatgct

8341 caagaaggaa ctttaattta tgatgatgta tctggatatg aatttgaaga atttgctaaa

8401 aaattaaaac ctgatttaat tgcttctgga attaaagaaa aatatgtatt tcaaaaaatg

8461 ggaatgcctt ttagacaaat gcattcttgg gattattctg gaccttatca tggatatgat

8521 ggatttgcta tttttgctag agatatggat ttagctttaa attctcctac ttggaattta

8581 attaaagctc cttggaaaca agctaaatag gatccacctg atctagagtc cgcaaaaatc

8641 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag

8701 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga

8761 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta

8821 aaaccaaaat ccagtgacgc ggccgctcgc taccttagga ccgttatagt taattaccct

8881 gttatcccta accggtcaac atgtggagca cgacacactt gtctactcca aaaatatcaa

8941 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg

9001 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa

9061 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc

9121 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga

9181 agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga

9241 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga

9301 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg

9361 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga

9421 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc

9481 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga

9541 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga

9601 cccttcctct atataaggaa gttcatttca tttggagagg acgtcgagag ttctcaacac

9661 aacatataca aaacaaacga atctcaagca atcaagcatt ctacttctat tgcagcaatt

9721 taaatcattt cttttaaagc aaaagcaatt ttctgaaaat tttcaccatt tacgaacgat

9781 agccatggct agcgctaccg gactcagatc tatggcttcc tctgtcattt cttcagcagc

9841 tgttgccaca cgcagcaatg ttacacaagc tagcatggtt gcacctttca ctggtctcaa

9901 atcttcagcc actttccctg ttacaaagaa gcaaaacctt gacatcactt ccattgctag

9961 caatggtgga agagttagct gcagagctca aatgtctcaa aatgtagata aaattaaaga

10021 tcattttcaa ttatttcaag aacctgaata tcaagaaatg tttgctagaa aaagagaatt

10081 tgaaggagga gcttctaaag aagaaattga aagagtaaga gaatggacta aatcttggga

10141 atatagagaa aaaaattttg ctagagaagc tttaactatt aatcctgcta aagcttgtca

10201 acctttagga gctatttttg ctgctgctgg atttgaagga actttacctt ttgtacatgg

10261 atctcaagga tgtgtagctt attttagatc tcatttaact agaaattata aagaaccttt

10321 tcaagctgta tcttcttcta tgactgaaga tgctgctgta tttggaggat taaaaaatat

10381 gattgatgga ttagctaatt cttatgcttt atataaacct aaaatgattg ctttatgtac

10441 tacttgtatg gctgaagtaa ttggagatga tttaggatct tttattacta attctaaaaa

10501 tgaaggagct gtacctcaag attttcctgt accttttgct catactcctt cttttgtagg

10561 atctcatatt actggatatg ataatatgtt aaaaggaatt ttaattgctt taactgatgg

10621 aaaaaaaact gaaactgata atggaaaaat taattttatt cctggatttg atccttatat

10681 tggaaatatt agagatttaa aaaatatttt atctttaatg gatgtacctt ctactgtatt

10741 agctgataat gctgaatctt ttgattctcc taatttagga gaatttaaaa tgtataatgg

10801 aggaactact ttagaagaag ctggagattc tattaatgct aaagctacta tttcttttca

10861 aaaatattct actcctaaaa ctttagaata tttaaaacaa gaaggaggac aaaaaactgc

10921 tacttataga cctattggag taagaggaac tgatgaattt ttaatggctt tatctgaatt

10981 aactggaaaa gctattcctg aagaattaga aattgaaaga ggaagagtag tagatgctat

11041 tactgattct caagcttggt tacatggaaa aagaattgct atttatggag atcctgatca

11101 tgtattagga ttattaaatt ttactttaga attaggaatg caacctgtac atgtagtagt

11161 aaataatgga aatgtagctg gatttgaaga agaagctaaa gaattattag ctaatgatcc

11221 taatggaaaa gaagctactg tatggattgg aaaagattta tggcatttaa gatctttatt

11281 agatactgaa cctgtagatt tattaattgg aaattcttat ggaaaatttt tacaaagaga

11341 tactggaact cctttagtaa gaattggata tcctattttt gatagacatc atcaacatag

11401 atattctatt ttaggatata aaggagcttt taatttaatt aattggattg taaatactat

11461 tttagatgaa ttagatagag gatctatgga tttaggagta aatgatactt cttttgattt

11521 agtaagataa ggatccacct gatctagagt ccgcaaaaat caccagtctc tctctacaaa

11581 tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta

11641 gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg

11701 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacg

11761 cggccgcatt accctgttat ccctattaat taagagctcg ctaccttaag agaggatatc

11821 ggcgcgcctc tagaatttaa atggatccta cgtactcgag gaattcaatt cggcgttaat

11881 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac

11941 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa

12001 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt

12061 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc

12121 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc

12181 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctcg ttcggttcgt

12241 aagctgtaat gcaagtagcg taactgccgt cacgcaactg gtccagaacc ttgaccgaac

12301 gcagcggtgg taacggcgca gtggcggttt tcatggcttc ttgttatgac atgttttttt

12361 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt

12421 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta

12481 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc

12541 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg

12601 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt

12661 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga

12721 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg

12781 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt

12841 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca

12901 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct

12961 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac

13021 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta

13081 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc

13141 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc

13201 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc

13261 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgccgg

13321 cgttaactca agcgattaga tgcactaagc acataattgc tcacagccaa actatcaggt

13381 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat

13441 atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt

13501 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca

13561 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct

13621 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta

13681 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct

13741 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc

13801 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca

13861 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga

13921 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg

13981 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt

14041 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag

14101 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt

14161 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt

14221 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga

14281 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca

14341 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac

14401 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct

14461 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc

14521 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt

14581 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag

14641 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga

14701 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc

14761 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt

14821 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg

14881 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta

14941 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc

15001 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc

15061 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc

15121 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa

15181 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc

15241 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc

15301 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc

15361 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt

15421 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt

15481 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc

15541 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc

15601 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat

15661 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat

15721 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc

15781 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact

15841 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt

15901 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat

15961 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg

16021 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat

16081 ttgtaccggg ccggatggtt tgcgaccgtc acgccgattc ctcgggcttg ggggttccag

16141 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc

16201 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc

16261 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc

16321 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc

16381 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct

16441 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc

16501 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa

16561 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa

16621 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa

16681 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgt

[표 5] SEQ. ID. NO. 48

PGE#0148 12181bp DNA 원형

유기체 E.Coli/식물에 폭격.

COMMENT pBS /PUC

특징 Location/Qualifiers

CDS 보체(11196..12053) AMPR

rep_origin 보체(10419..11033) ORIPUC

misc_feature 보체(8485..9974) Tom\Chl\2

misc_feature 659..2582 Tom\CHL\1

CDS 보체(8675..8745) tRNA-G

CDS 2495..2568 tRNA-fM

터미네이터 8220..8414 PsbA\trminator

프로모터 2644..2836 rrn16P

CDS 6941..7870 aadA

RBS 3734..3813 인공\SD

RBS 5312..5327 인공\SD

RBS 6871..6939 인공\SD

CDS 2837..3727 인공\NifH\912

CDS 3815..5305 인공\NifD912

CDS 5328..6863 인공\NifK\912

1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct

61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg

121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact

181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac

241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga

301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga

361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca

421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg

481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg

541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg

601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg

661 attggggcgt ggacataagg gtctttatga cacaatcaac aattcgcttc attttcaatt

721 aggccttgct ctagcttctt taggggttat tacttctttg gtagctcaac acatgtactc

781 tttacctgct tatgcattca tagcacaaga ctttactact caagctgcat tatataccca

841 ccaccaatat atcgcaggat tcatcatgac aggagctttt gctcatggag ctatattttt

901 cattagagat tacaatccgg agcaaaatga agataatgta ttggcaagaa tgttagatca

961 taaagaagct atcatatctc atttaagttg ggccagcctc tttctgggat tccataccct

1021 gggactttat gttcataatg atgtcatgct tgcctttggc actccggaga agcaaatctt

1081 gattgaaccg atatttgctc aatggataca atccgctcat ggtaaaactt catatgggtt

1141 cgatgtactt ttatcttcaa cgactggccc agcattcaat gcgggtcgaa gcatctggtt

1201 gccgggttgg ttaaatgctg ttaatgaaaa tagtaattca ttattcttaa caataggtcc

1261 tggagacttt ttggttcatc atgctattgc ccttggttta catacaacta cattgatctt

1321 agtaaaaggt gctttagatg cacgtggttc caagttaatg ccagataaaa aggatttcgg

1381 ttatagtttt ccgtgcgatg gcccaggacg aggcggtact tgtgatattt cggcatggga

1441 cgcgttttat ttggcagttt tttggatgtt aaatactatt ggatgggtta ctttttattg

1501 gcattggaag cacatcacat tatggcaagg taacgtttca cagtttaatg aatcttccac

1561 ttatttgatg ggctggttaa gggattattt atggttaaac tcttcacaac ttatcaatgg

1621 atataaccct tttggtatga atagtttatc ggtttgggca tggatgttct tatttggaca

1681 tcttgtttgg gctactggat ttatgttctt aatttcttgg cgtggatatt ggcaggaatt

1741 gattgaaact ttagcatggg ctcacgaacg cacacctttg gccaatttga ttcgatggag

1801 agataaacca gtggcccttt ctattgtaca agcaagattg gttggattag ctcacttttc

1861 tgtaggttat atattcactt atgcggcttt cttgattgcc tctacgtcgg gcaaatttgg

1921 ttaattaatg tgtgtattcg cgataatctc atttctttcg acggagaagg gggtccacct

1981 tcttctattt ctacatctag gattcgactt gtatcatgga tactaatagg aattcaacca

2041 ttatggcaag gaaaagtttg attcagaggg agaagaagag gcaaaaattg gaacagaaat

2101 atcattcgat tcgtcgatcc tcaaaaaaag aaataagcaa ggttccgtcg ttgagtgaca

2161 aatgggaaat ttatggaaag ttacaatccc taccacggaa tagtgcacct acacgccttc

2221 atcgacgttg ttttttgacc ggaaggccga gagctaacta tcgagacttt ggcctatccg

2281 gacacatact tcgtgaaatg gttcatgcat gtttgttgcc aggagcaaca agatcaagtt

2341 ggtaaggatt aacgcttcat ttctatttct atggtcgatg atcatagaag cccctttacc

2401 attctgtata aatgggctat tctatttgta cagatagggt ggaggggcgc atttaatcct

2461 tgtttatcta ttagttttca gttcttatct ttggcgcggg gtagagcagt ttggtagctc

2521 gcaaggctca taaccttgag gtcacgggtt caaatcctgt ctccgcaaca tcttattctg

2581 gtctcgaggt ccgcataaag aaccacccat aatacccata atagctgttt gccaaccggt

2641 cgccgtcgtt caatgagaat ggataagagg ctcgtgggat tgacgtgagg gggcagggat

2701 gactatattt ctgggagcga actccgggcg aatatgaagc gcatggatac aagttatgcc

2761 ttggaatgaa agacaattcc gaatccgctt tgtctacgaa caaggaagct ataagtaatg

2821 caactatgaa ggatctatgt ctatcgataa gaaaatccgt caaatcgcat tttacggaaa

2881 aggcggtatc ggaaaatcca ctacctcaca aaacactctt gctgcaatgg ctgaaatggg

2941 tcaaagaatc cttatcgttg gatgcgatcc taaagcagac agtacccgcc ttatgcttca

3001 ctctaaagct caaaccaccg tacttcatct tgcagctgaa cgtggtgctg ttgaagatct

3061 tgaattagag gaagtaatgt taactggttt tagaggagtt aaatgcgtag agtctggtgg

3121 tccagaacca ggtgttggat gtgcaggtcg aggaatcatc actgctatca atttcttaga

3181 ggaaaacggt gcatatcaag atgtagattt tgtttcatac gatgttttag gtgatgtagt

3241 ttgtggtgga ttcgctatgc cgatccgtga gaacaaagca caagaaatct atatcgtaac

3301 cagtggtgag atgatggcta tgtacgcagc taataacatc gctagaggta tcttaaagta

3361 tgcacatact ggaggggttc gattaggcgg gttaatctgt aattcacgta acgtagatcg

3421 tgaaatcgag ttgatcgaaa ctttggctaa aagattgaat actcaaatga tccattacgt

3481 tcctcgcgat aacatcgtac aacatgccga gttgcgtcgc atgacagtaa atgaatatgc

3541 ccctgattcc aatcagtcta atgagtatcg tatcttggcg aataaaatca tcaataatga

3601 aaatttgaaa gtacctactc ctatcgagat ggaagaacta gaagaactac tgatcgaatt

3661 tggtatcctg gaatctgaag aaaatgctgc taaaatgatc gggactcctg ctcagtcttc

3721 cactaaataa tagcggccgc aacccataat acccataata gctgtttgcc atcgctacct

3781 taggaccgtt atagttaacc ggtggaggca gactatgact cctccagaaa atcaaaacct

3841 agtagatgag aataaagagt taatcaaaga agtgcttcaa gcttaccctg aaaaggcacg

3901 aaaaaaaaga gagaagcatt taaatgttca cgaagaaagt aaatccgatt gtggtgtaaa

3961 atctaacata aagtccattc caggcgttat gactgctcgt gggtgcgcat atgctggtag

4021 caaaggggta gtttggggtc ctatcaaaga tatgattcat atatcccacg ggcctgtagg

4081 ttgtgggtac tggtcttggt ccggaagacg taattattac gttggtatca ccggaattaa

4141 ctctttcggt actatgcatt ttacttccga tttccaggaa agagacatcg tatttggagg

4201 tgataagaaa ttgaccaaaa taattgatga gttagaaatc ctttttccat taaatcgtgg

4261 agtgtctatt caatcagaat gccctatcgg tttgattgga ggtgatatcg aagcagttgc

4321 taaaaaagca tctaagcagt atggaaaacc agtagttcct ttaagatgtg agggtctgcg

4381 tggagtatca caatctttag gtcatcacat tgctaacgac gcaatcagag attggatttt

4441 cccagaattt gataaggcta aaaaaaataa tactatcgac tttgaacctt caccgtacga

4501 tgttgcactt attggagatc ataacatcgg tggagatgct tgggcatctc gtatgttatt

4561 ggaggaaatg ggtctgagag tagttgctca atggtcagga gatggtactt taaatgaact

4621 tattcaagga cctgcagcta aattggtact gattcactgc tatcgttcta tgaattacat

4681 ctgtcgctca ttggaggaac aatatggtat gccgtggatg gaattcaact tttttggacc

4741 tactaaaatt gcagcttctc ttcgtgagat cgcagctaaa ttcgacagta aaattcaaga

4801 aaatgcagaa aaagttatcg ctaaatacac cccggtaatg aatgcagtgc ttgagaaata

4861 tcgccctcgt ttggaaggta acactgttat gctttacgta gggggtcttc gccctcgtca

4921 tgttgtacct gctttcgaag atttggggat taaagttgta ggtaccgggt atgaatttgc

4981 acataatgat gattacaaac gcactacaca ctatatcgat aacgctacta ttatctacga

5041 tgatgttaca gcctatgagt tcgaagaatt tgtaaaagct aaaaaacctg atcttattgc

5101 ctctggtatc aaagaaaaat acgcttttca gaaaatgggt ctaccttttc gacaaatgca

5161 tagttgggat tattctggtc cttatcatgg ttatgatcgt tttgccatct ttgctcgaga

5221 tatggatctt gccctaaata gtcctacttg gagtctaatc ggtgcgcctt ggaaaaaagc

5281 ggctactaaa gcgaaagctg ctgcttaata actcgagaga tacaacaatg cctcaaaatc

5341 cagagaaaat tgtagatcat gtacaattat ttcagcaacc tgaatatcaa gaacttttca

5401 aaaataaaca cgaacaattt gagggagctc attccccaga agaagtacag agagtatccg

5461 aatggactaa aggttgggag tacagagaaa agaactttgc acgtcaagct ttaacagtaa

5521 atcctgcaaa aggatgccaa ccagtaggtg ctatcttcgc agctgtagga tttgaaggta

5581 ctctaccttt cgtacaagga tcccagggtt gtgtagcata ttttagaaca catttatccc

5641 gtcactacaa agaaccattc tctgctgtat cctcatctat gactgaggat gcagctgtat

5701 ttggaggtct taacaatatg attgaaggat tacaagtatc atatcaacta tacaaaccta

5761 aaatgatcgc agtatgtaca acttgtatgg ctgaagtaat tggtgatgat ttaggagcat

5821 tcatcacaaa tgctaaaaac gcaggttcta ttccacaaga ttttcctgta ccattcgctc

5881 atactccttc atttgtagga tctcatacaa ccggttatga taatatgatg aagggaatcc

5941 tttcaaattt aactgagggt aaaaaaaaag ataagaccaa cggaaaaatt aatttcatcc

6001 caggttttga tacttacgta ggaaataaca gagaactgaa acgtatgtta ggtcttatgg

6061 gaattgatta taccatctta gcagataatt ctgattacct ggatgctcct aatactggtg

6121 aatatgatat gtacccgggc ggtaccaaat tagaggatgc agctgattca tgcaacgcag

6181 aagctactgt agcccttcag agatatacca ctaccaagac tcgagaatac attgagacca

6241 aatggaaaca acaaactaaa gtattacgtc ctttcggggt aaaaggtacc gacgaatttc

6301 ttatgacttt atctgaaatg accgggaaac cgatccctca agaacttgag gatgaacgag

6361 gtcgtttagt agacgctatg actgatagtt atgcctggat tcacgggaaa aaattcgcta

6421 tctacggtga tccggacctt atttattctg taaccagttt tttacttgaa ttaggggccg

6481 aacctgtaca tatcctttgt aataatggtg atgaagagtt caaaaaagag atggaagcta

6541 ttttatctgc gagtccgttt gggaaacaag ctactgtatg gatccagaaa gatttgtggc

6601 atcttcgatc tttgcttttt actgaacctg tagatttttt tgtaggtaac agttacggga

6661 aatatttgtg gcgcgacacc aaaattccta tggtacgtat cggttatcct ctttttgatc

6721 gccaccattt gcatcgttat cctactttgg ggtatcaagg tggtttgaat ttgcttaatt

6781 gggtagtaaa cactattttg gatgaacttg atcgcagtag caatatagcg ggtaaaactg

6841 atatatcttt tgatttgatc cgttaatagg cggccgctcg ctaccttagg accgttatag

6901 ttattaccct gttatcccta accggtggag gcttcttgtt atgacatgtt tttttggggt

6961 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat

7021 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat

7081 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat

7141 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg

7201 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga

7261 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag

7321 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg

7381 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg

7441 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga

7501 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca

7561 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc

7621 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg

7681 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta

7741 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc

7801 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt

7861 agtcggcaaa taatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc

7921 gggatccacc tgatctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct

7981 ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta

8041 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa

8101 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac gcggccgcat

8161 taccctgtta tccctattaa ttaagagctc gctaccttaa gagaggatat cggcgcgccg

8221 atcctagcct agtctatagg aggttttgaa aagaaaggag caataatcat tttcttgttc

8281 tgtcaagagg gtgctattgc tcctttcttt tttttttttt ctttactaat ttcctagtat

8341 tttactgaca tagacttttt tgtttacatt atcgaaaaag aaagagaggg tatttgcttg

8401 catttattca tgatggatcc cccgggctgc aggcatgcaa gctaattccc gatctagtaa

8461 catagatgac accgcgcgcc gcggaactag taattaattc ccgcctttcg ctttttgggg

8521 gtggaaggca aaagaaaacg taggggaggg atagaatcac tacactatca cggccaacta

8581 taccaactcc ttaatgtaag gatatattta atgctattta tgaaattcaa taataaaaag

8641 aaatagtaaa aaaattactt tatcttggat cttgggcgga tagcgggaat cgaacccgca

8701 tcttctcctt ggcaaagaga aattttacca ttcgaccata tccgcatttt tttgttcttg

8761 atacacaata tgtacccaca tatatgatat ataaccggat cttatttgtg cagtgccggg

8821 acacatattc tcttcggaac gattccaata attttgttaa ttatattctt tttattcaag

8881 aagtttgacc cccctctaat ttttttgttt tctttatttg atttgcattt tctttgggga

8941 cttagattca aatttaatgt gtctcacaac cgagaaaaat taggggggtc attttggttt

9001 tgggtctgcg acgaataggt tcaagagatg agagaattaa ggatacccac cagaaagact

9061 aatccaatcc ataaggaggt accagaaaat acaacatttt tgttacttga ccagccatca

9121 ggagaagcaa atacaacggg tacgctaatc aataagatta atgaagtagc aattaatgca

9181 aaaacagcca attggaaagc aagagtcatg cttttaatcc tccaagctac caacaaatga

9241 actatatacc atttgatccc tctatcagcc aaaaaatatt aattgtgata aaatatgtca

9301 tcgagggatt ttactttatc atgaatccat tgattctata tgacttatta ctactccccc

9361 tttcgcactt tattcgtaca tggagtgggg tggggggaaa tggaattttc ttttttattt

9421 cacaaatgga catgctagat catatatcta tatacggata gatagatcga tcggcggatt

9481 cgcacctgag atctttctac agatagtggg ggtatccacc cctatagcca tgttctattc

9541 ggaggaataa aataaaaata gtctttcgga gagatggctg agtggttgat agccccggtc

9601 ttgaaaaccg gtatagtttt gaacaaagaa ctatcgaggg ttcgaatccc tctctctcct

9661 tttttgctaa ttgaatagat ttttttattt agtggttttg cccaatctgc tatccgaaag

9721 aaaagggaat ggctcggcta tcccacctag ccaagccaga aaaatagatt agatataaat

9781 tagataaaat aaatgagttg aaaaaaaaaa aagaaaaaag gaatacttaa gctgattcca

9841 agatgtatga ttgaatcaaa gtaatttgta cttcattcaa gcattggatc tcctgtctca

9901 tatcaattaa gaggggtcat ggaaagaaca ggttcaaagt cgcgatcaat tcctttttca

9961 aatcctgctg cagcgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg

10021 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac

10081 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc

10141 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg

10201 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct

10261 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac

10321 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga

10381 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat

10441 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac

10501 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct

10561 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg

10621 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg

10681 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt

10741 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg

10801 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac

10861 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga

10921 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt

10981 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt

11041 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga

11101 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc

11161 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct

11221 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata

11281 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca

11341 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga

11401 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga

11461 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg

11521 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga

11581 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt

11641 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct

11701 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca

11761 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat

11821 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga

11881 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc

11941 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg

12001 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc

12061 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt

12121 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca

12181 c

[표 6] SEQ. ID. NO. 49

PGE#0048 16791bp DNA 원형

공급원 식물 유전자 조작 인공

숙주 유기체 E.coli/아그로박테리움

표적 유기체 토마토 또는 다른 식물 일시적 및 안정한 형질전환.

특징 Location/Qualifiers

CDS 12370..13338 spectinomycin\R

repeat_region 13790..14070 ORI\E1

rep_origin 보체(14880..15880) PVS1\rep

misc_feature 1730..1797 RB

misc_feature 보체(11990..12033) LB

misc_recomb 1828..3676 Reporter NptII

CDS 보체 (2544..3335) NptII

CDS 합성 DNA 9837..11591 TP-NifK912

CDS 합성 DNA 6961..8658 TP-NifD912

CDS 합성 DNA 4661..5770 TP-NifH912

프로모터 3773..4660 2X35S

터미네이터 5774..6060 Trminator

프로모터 6073..6960 2X35S

터미네이터 8663..8939 터미네이터

프로모터 8951..9836 2X35S

기원 합성 DNA

1 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga

61 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc

121 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg

181 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc

241 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg

301 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga

361 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt

421 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat

481 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag

541 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc

601 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa

661 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc

721 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc

781 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg

841 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc

901 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg

961 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc

1021 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg

1081 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg

1141 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg

1201 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca

1261 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc

1321 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg

1381 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc

1441 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag

1501 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca

1561 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc

1621 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg

1681 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta

1741 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga

1801 caatctgatc caagctcaag ctaagcttca gagccaccac cctcagagcc gccaccagaa

1861 ccaccaccag agccgccgcc agcattgaca ggaggcccga tctagtaaca tagatgacac

1921 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat

1981 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat

2041 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc

2101 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggatcat

2161 ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtccat

2221 ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccgg gcccgatcat

2281 attgtcgctc aggatcgtgg cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc

2341 accttcgacc gcctgttccg cagagatccc gtgggcgaag aactccagca tgagatcccc

2401 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata

2461 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat

2521 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc

2581 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca

2641 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc

2701 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag

2761 caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg

2821 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca

2881 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat

2941 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact

3001 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc

3061 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc

3121 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg

3181 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca

3241 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc

3301 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccaga tccggtgcag

3361 attatttgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa

3421 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt

3481 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg

3541 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc

3601 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcagattg

3661 tcgtttcccg ccttcaggta ccgcgatcgc tcgcgacctg caggcataaa gccgtcagtg

3721 tccgcataaa gaaccaaccc ataataccca taatagctgt ttgccaaccg gtcaacatgt

3781 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag

3841 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc

3901 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca

3961 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga

4021 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa

4081 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat

4141 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc

4201 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga

4261 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga

4321 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa

4381 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt

4441 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc

4501 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct

4561 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa

4621 gcaattttct gaaaattttc accatttacg aacgatagcc atggctagcg ctaccggact

4681 cagatctatg gcttcctctg tcatttcttc agcagctgtt gccacacgca gcaatgttac

4741 acaagctagc atggttgcac ctttcactgg tctcaaatct tcagccactt tccctgttac

4801 aaagaagcaa aaccttgaca tcacttccat tgctagcaat ggtggaagag ttagctgcag

4861 agctcaagct tcgaattcta tgtctatcga taagaaaatc cgtcaaatcg cattttacgg

4921 aaaaggcggt atcggaaaat ccactacctc acaaaacact cttgctgcaa tggctgaaat

4981 gggtcaaaga atccttatcg ttggatgcga tcctaaagca gacagtaccc gccttatgct

5041 tcactctaaa gctcaaacca ccgtacttca tcttgcagct gaacgtggtg ctgttgaaga

5101 tcttgaatta gaggaagtaa tgttaactgg ttttagagga gttaaatgcg tagagtctgg

5161 tggtccagaa ccaggtgttg gatgtgcagg tcgaggaatc atcactgcta tcaatttctt

5221 agaggaaaac ggtgcatatc aagatgtaga ttttgtttca tacgatgttt taggtgatgt

5281 agtttgtggt ggattcgcta tgccgatccg tgagaacaaa gcacaagaaa tctatatcgt

5341 aaccagtggt gagatgatgg ctatgtacgc agctaataac atcgctagag gtatcttaaa

5401 gtatgcacat actggagggg ttcgattagg cgggttaatc tgtaattcac gtaacgtaga

5461 tcgtgaaatc gagttgatcg aaactttggc taaaagattg aatactcaaa tgatccatta

5521 cgttcctcgc gataacatcg tacaacatgc cgagttgcgt cgcatgacag taaatgaata

5581 tgcccctgat tccaatcagt ctaatgagta tcgtatcttg gcgaataaaa tcatcaataa

5641 tgaaaatttg aaagtaccta ctcctatcga gatggaagaa ctagaagaac tactgatcga

5701 atttggtatc ctggaatctg aagaaaatgc tgctaaaatg atcgggactc ctgctcagtc

5761 ttccactaaa taaggatcca cctgatctag agtccgcaaa aatcaccagt ctctctctac

5821 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa

5881 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat

5941 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg

6001 acgcggccgc acccataata cccataatag ctgtttgcca tcgctacctt aggaccgtta

6061 tagttaaccg gtcaacatgt ggagcacgac acacttgtct actccaaaaa tatcaaagat

6121 acagtctcag aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac

6181 ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa

6241 ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct

6301 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac

6361 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca

6421 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact

6481 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac

6541 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa

6601 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc

6661 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga

6721 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct

6781 tcctctatat aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca

6841 tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa

6901 tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagcc

6961 atggctagcg ctaccggact cagatctatg gcttcctctg tcatttcttc agcagctgtt

7021 gccacacgca gcaatgttac acaagctagc atggttgcac ctttcactgg tctcaaatct

7081 tcagccactt tccctgttac aaagaagcaa aaccttgaca tcacttccat tgctagcaat

7141 ggtggaagag ttagctgcag agctcaaatg actcctccag aaaatcaaaa cctagtagat

7201 gagaataaag agttaatcaa agaagtgctt caagcttacc ctgaaaaggc acgaaaaaaa

7261 agagagaagc atttaaatgt tcacgaagaa agtaaatccg attgtggtgt aaaatctaac

7321 ataaagtcca ttccaggcgt tatgactgct cgtgggtgcg catatgctgg tagcaaaggg

7381 gtagtttggg gtcctatcaa agatatgatt catatatccc acgggcctgt aggttgtggg

7441 tactggtctt ggtccggaag acgtaattat tacgttggta tcaccggaat taactctttc

7501 ggtactatgc attttacttc cgatttccag gaaagagaca tcgtatttgg aggtgataag

7561 aaattgacca aaataattga tgagttagaa atcctttttc cattaaatcg tggagtgtct

7621 attcaatcag aatgccctat cggtttgatt ggaggtgata tcgaagcagt tgctaaaaaa

7681 gcatctaagc agtatggaaa accagtagtt cctttaagat gtgagggtct gcgtggagta

7741 tcacaatctt taggtcatca cattgctaac gacgcaatca gagattggat tttcccagaa

7801 tttgataagg ctaaaaaaaa taatactatc gactttgaac cttcaccgta cgatgttgca

7861 cttattggag atcataacat cggtggagat gcttgggcat ctcgtatgtt attggaggaa

7921 atgggtctga gagtagttgc tcaatggtca ggagatggta ctttaaatga acttattcaa

7981 ggacctgcag ctaaattggt actgattcac tgctatcgtt ctatgaatta catctgtcgc

8041 tcattggagg aacaatatgg tatgccgtgg atggaattca acttttttgg acctactaaa

8101 attgcagctt ctcttcgtga gatcgcagct aaattcgaca gtaaaattca agaaaatgca

8161 gaaaaagtta tcgctaaata caccccggta atgaatgcag tgcttgagaa atatcgccct

8221 cgtttggaag gtaacactgt tatgctttac gtagggggtc ttcgccctcg tcatgttgta

8281 cctgctttcg aagatttggg gattaaagtt gtaggtaccg ggtatgaatt tgcacataat

8341 gatgattaca aacgcactac acactatatc gataacgcta ctattatcta cgatgatgtt

8401 acagcctatg agttcgaaga atttgtaaaa gctaaaaaac ctgatcttat tgcctctggt

8461 atcaaagaaa aatacgcttt tcagaaaatg ggtctacctt ttcgacaaat gcatagttgg

8521 gattattctg gtccttatca tggttatgat cgttttgcca tctttgctcg agatatggat

8581 cttgccctaa atagtcctac ttggagtcta atcggtgcgc cttggaaaaa agcggctact

8641 aaagcgaaag ctgctgctta aggatccacc tgatctagag tccgcaaaaa tcaccagtct

8701 ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga

8761 taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta

8821 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa

8881 atccagtgac gcggccgctc gctaccttag gaccgttata gttaattacc ctgttatccc

8941 taaccggtca acatgtggag cacgacacac ttgtctactc caaaaatatc aaagatacag

9001 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc

9061 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg

9121 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg

9181 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc

9241 caaccacgtc ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacacacttg

9301 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc

9361 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta

9421 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa

9481 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga

9541 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg

9601 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct

9661 ctatataagg aagttcattt catttggaga ggacgtcgag agttctcaac acaacatata

9721 caaaacaaac gaatctcaag caatcaagca ttctacttct attgcagcaa tttaaatcat

9781 ttcttttaaa gcaaaagcaa ttttctgaaa attttcacca tttacgaacg atagccatgg

9841 ctagcgctac cggactcaga tctatggctt cctctgtcat ttcttcagca gctgttgcca

9901 cacgcagcaa tgttacacaa gctagcatgg ttgcaccttt cactggtctc aaatcttcag

9961 ccactttccc tgttacaaag aagcaaaacc ttgacatcac ttccattgct agcaatggtg

10021 gaagagttag ctgcagagct caagcttcga attctatgcc tcaaaatcca gagaaaattg

10081 tagatcatgt acaattattt cagcaacctg aatatcaaga acttttcaaa aataaacacg

10141 aacaatttga gggagctcat tccccagaag aagtacagag agtatccgaa tggactaaag

10201 gttgggagta cagagaaaag aactttgcac gtcaagcttt aacagtaaat cctgcaaaag

10261 gatgccaacc agtaggtgct atcttcgcag ctgtaggatt tgaaggtact ctacctttcg

10321 tacaaggatc ccagggttgt gtagcatatt ttagaacaca tttatcccgt cactacaaag

10381 aaccattctc tgctgtatcc tcatctatga ctgaggatgc agctgtattt ggaggtctta

10441 acaatatgat tgaaggatta caagtatcat atcaactata caaacctaaa atgatcgcag

10501 tatgtacaac ttgtatggct gaagtaattg gtgatgattt aggagcattc atcacaaatg

10561 ctaaaaacgc aggttctatt ccacaagatt ttcctgtacc attcgctcat actccttcat

10621 ttgtaggatc tcatacaacc ggttatgata atatgatgaa gggaatcctt tcaaatttaa

10681 ctgagggtaa aaaaaaagat aagaccaacg gaaaaattaa tttcatccca ggttttgata

10741 cttacgtagg aaataacaga gaactgaaac gtatgttagg tcttatggga attgattata

10801 ccatcttagc agataattct gattacctgg atgctcctaa tactggtgaa tatgatatgt

10861 acccgggcgg taccaaatta gaggatgcag ctgattcatg caacgcagaa gctactgtag

10921 cccttcagag atataccact accaagactc gagaatacat tgagaccaaa tggaaacaac

10981 aaactaaagt attacgtcct ttcggggtaa aaggtaccga cgaatttctt atgactttat

11041 ctgaaatgac cgggaaaccg atccctcaag aacttgagga tgaacgaggt cgtttagtag

11101 acgctatgac tgatagttat gcctggattc acgggaaaaa attcgctatc tacggtgatc

11161 cggaccttat ttattctgta accagttttt tacttgaatt aggggccgaa cctgtacata

11221 tcctttgtaa taatggtgat gaagagttca aaaaagagat ggaagctatt ttatctgcga

11281 gtccgtttgg gaaacaagct actgtatgga tccagaaaga tttgtggcat cttcgatctt

11341 tgctttttac tgaacctgta gatttttttg taggtaacag ttacgggaaa tatttgtggc

11401 gcgacaccaa aattcctatg gtacgtatcg gttatcctct ttttgatcgc caccatttgc

11461 atcgttatcc tactttgggg tatcaaggtg gtttgaattt gcttaattgg gtagtaaaca

11521 ctattttgga tgaacttgat cgcagtagca atatagcggg taaaactgat atatcttttg

11581 atttgatccg ttaagatcca cctgatctag agtccgcaaa aatcaccagt ctctctctac

11641 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa

11701 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat

11761 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg

11821 acgcggccgc attaccctgt tatccctatt aattaagagc tcgctacctt aagagaggat

11881 atcggcgcgc ctctagaatt taaatggatc ctacgtactc gaggaattca attcggcgtt

11941 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt

12001 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac

12061 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt

12121 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct

12181 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag

12241 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tcgttcggtt

12301 cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg

12361 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat gacatgtttt

12421 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga

12481 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag

12541 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt

12601 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca

12661 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg

12721 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct

12781 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt

12841 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt

12901 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa

12961 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt

13021 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc

13081 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca

13141 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg

13201 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat

13261 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc

13321 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc

13381 cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc caaactatca

13441 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga

13501 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc

13561 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt

13621 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac

13681 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt

13741 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct

13801 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga

13861 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac

13921 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg

13981 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt

14041 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc

14101 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg

14161 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc

14221 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc

14281 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag

14341 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc

14401 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta

14461 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc

14521 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc

14581 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag

14641 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg

14701 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg

14761 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc

14821 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag

14881 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat

14941 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat

15001 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt

15061 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc

15121 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc

15181 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca

15241 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct

15301 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg

15361 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat

15421 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct

15481 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc

15541 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt

15601 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc

15661 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt

15721 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc

15781 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac

15841 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg

15901 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc

15961 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg

16021 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg

16081 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc

16141 gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc

16201 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc

16261 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc

16321 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc

16381 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc

16441 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg

16501 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt

16561 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt

16621 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa

16681 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact

16741 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg t

여기 개시된 과정 및 구체예들에 다양한 변형이 이루어질 수 있다는 것이 이해될 것이다. 따라서, 상기 설명은 제한으로 해석되어서는 안되며, 다양한 구체예의 예시로서만 해석되어야 한다. 당업자는 여기 첨부된 청구항의 범위 및 사상 내에서 다른 변형들도 생각할 수 있을 것이다.

SEQUENCE LISTING <110> Zaltsman, Adi <120> PLANT SELF NITROGEN FIXATION BY MIMICKING PROKARYOTIC PATHWAYS <130> 1645-2-prov <140> 61/732,490 <141> 2012-12-03 <160> 49 <170> PatentIn version 3.5 <210> 1 <211> 12166 <212> DNA <213> Escherichia coli <400> 1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 60 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 120 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 180 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 240 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 300 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 360 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 420 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg 660 attggggcgt ggacataagg gtctttatga cacaatcaac aattcgcttc attttcaatt 720 aggccttgct ctagcttctt taggggttat tacttctttg gtagctcaac acatgtactc 780 tttacctgct tatgcattca tagcacaaga ctttactact caagctgcat tatataccca 840 ccaccaatat atcgcaggat tcatcatgac aggagctttt gctcatggag ctatattttt 900 cattagagat tacaatccgg agcaaaatga agataatgta ttggcaagaa tgttagatca 960 taaagaagct atcatatctc atttaagttg ggccagcctc tttctgggat tccataccct 1020 gggactttat gttcataatg atgtcatgct tgcctttggc actccggaga agcaaatctt 1080 gattgaaccg atatttgctc aatggataca atccgctcat ggtaaaactt catatgggtt 1140 cgatgtactt ttatcttcaa cgactggccc agcattcaat gcgggtcgaa gcatctggtt 1200 gccgggttgg ttaaatgctg ttaatgaaaa tagtaattca ttattcttaa caataggtcc 1260 tggagacttt ttggttcatc atgctattgc ccttggttta catacaacta cattgatctt 1320 agtaaaaggt gctttagatg cacgtggttc caagttaatg ccagataaaa aggatttcgg 1380 ttatagtttt ccgtgcgatg gcccaggacg aggcggtact tgtgatattt cggcatggga 1440 cgcgttttat ttggcagttt tttggatgtt aaatactatt ggatgggtta ctttttattg 1500 gcattggaag cacatcacat tatggcaagg taacgtttca cagtttaatg aatcttccac 1560 ttatttgatg ggctggttaa gggattattt atggttaaac tcttcacaac ttatcaatgg 1620 atataaccct tttggtatga atagtttatc ggtttgggca tggatgttct tatttggaca 1680 tcttgtttgg gctactggat ttatgttctt aatttcttgg cgtggatatt ggcaggaatt 1740 gattgaaact ttagcatggg ctcacgaacg cacacctttg gccaatttga ttcgatggag 1800 agataaacca gtggcccttt ctattgtaca agcaagattg gttggattag ctcacttttc 1860 tgtaggttat atattcactt atgcggcttt cttgattgcc tctacgtcgg gcaaatttgg 1920 ttaattaatg tgtgtattcg cgataatctc atttctttcg acggagaagg gggtccacct 1980 tcttctattt ctacatctag gattcgactt gtatcatgga tactaatagg aattcaacca 2040 ttatggcaag gaaaagtttg attcagaggg agaagaagag gcaaaaattg gaacagaaat 2100 atcattcgat tcgtcgatcc tcaaaaaaag aaataagcaa ggttccgtcg ttgagtgaca 2160 aatgggaaat ttatggaaag ttacaatccc taccacggaa tagtgcacct acacgccttc 2220 atcgacgttg ttttttgacc ggaaggccga gagctaacta tcgagacttt ggcctatccg 2280 gacacatact tcgtgaaatg gttcatgcat gtttgttgcc aggagcaaca agatcaagtt 2340 ggtaaggatt aacgcttcat ttctatttct atggtcgatg atcatagaag cccctttacc 2400 attctgtata aatgggctat tctatttgta cagatagggt ggaggggcgc atttaatcct 2460 tgtttatcta ttagttttca gttcttatct ttggcgcggg gtagagcagt ttggtagctc 2520 gcaaggctca taaccttgag gtcacgggtt caaatcctgt ctccgcaaca tcttattctg 2580 gtctcgaggt ccgcataaag aaccacccat aatacccata atagctgttt gccaaccggt 2640 cgccgtcgtt caatgagaat ggataagagg ctcgtgggat tgacgtgagg gggcagggat 2700 gactatattt ctgggagcga actccgggcg aatatgaagc gcatggatac aagttatgcc 2760 ttggaatgaa agacaattcc gaatccgctt tgtctacgaa caaggaagct ataagtaatg 2820 caactatgaa ggatctatga ctgacgaaaa cattagacag atagctttct acggtaaagg 2880 cggtatcggt aaatctacca cctcccaaaa cacccttgca gctatggcag aaatgggtca 2940 acgcatcatg attgtaggtt gcgaccctaa agctgactcc acccgtctga tgcttcactc 3000 caaagctcaa accaccgtac tacacttagc tgctgaacgc ggtgcagtag aagacttaga 3060 actccacgaa gtaatgttga ccggtttccg tggcgttaag tgcgtagaat ctggtggtcc 3120 agaacccggt gtaggttgcg ccggtcgtgg tatcatcacc gccattaact tcttagaaga 3180 aaacggcgct taccaagacc tagacttcgt atcctacgac gtattgggtg acgttgtatg 3240 tggtggtttc gctatgccta tccgtgaagg taaagcacaa gaaatctaca tcgttacctc 3300 tggtgaaatg atggcgatgt atgctgctaa caacatcgct cgcggtattt tgaaatatgc 3360 tcactccggt ggtgtacgtt taggtggttt gatctgtaac agccgtaagg ttgaccgtga 3420 agacgagtta atcatgaact tggctgaacg tttgaacacc caaatgattc acttcgtacc 3480 tcgtgacaac atcgttcaac acgcagaatt gcgccgtatg accgttaacg agtacgcacc 3540 agacagcaac caaggtcaag agtaccgcgc attagctaag aagatcatca acaacgacaa 3600 gctcaccatt cctacaccaa tggaaatgga tgaactagaa gctctgttga tcgaatacgg 3660 tctattagac gacgacacca agcactctga aatcatcggt aagcccgcag aagctaccaa 3720 atagcggccg caacccataa tacccataat agctgtttgc catcgctacc ttaggaccgt 3780 tatagttaac cggtggaggc agactatgac acctcctgaa aacaagaatc ttgtagatga 3840 aaataaggaa cttattcaag aagttctgaa agcttatccc gaaaaatctc gcaaaaagcg 3900 cgaaaaacac ctcaacgtcc acgaagaaaa caagtctgat tgcggcgtta agtctaacat 3960 caaatccgtt cctggtgtaa tgaccgcccg tggttgtgct tatgcaggtt ctaagggtgt 4020 ggtttggggt cctattaagg acatgatcca catcagccac gggcctgtag gttgcggtta 4080 ctggtcttgg tctggtcgtc gtaactacta cgttggtgta actggtatca actctttcgg 4140 taccatgcac ttcacctcag acttccaaga acgtgacatc gtgttcggtg gtgacaaaaa 4200 actcactaaa ctcattgaag aactcgatgt tcttttccct ctcaaccgtg gtgtttccat 4260 tcaatctgaa tgtcccattg gtctaattgg ggatgacatc gaagctgttg ctaagaaaac 4320 ttctaagcaa attggtaagc ctgttgtacc cttacgttgc gaaggtttcc gtggtgtgtc 4380 tcagtcctta ggacaccaca tcgctaacga cgctatccgt gactggattt tcccagaata 4440 cgacaagctc aagaaagaaa acagacttga cttcgagcca agcccctatg atgtagctct 4500 aatcggtgac tacaacatcg gtggtgacgc ttgggccagc cggatgctgt tggaagaaat 4560 gggcttacgt gtagtagctc agtggtctgg tgatggtaca ctcaacgagt tgatccaagg 4620 ccctgctgct aagttagtcc tcatccactg ctaccgttct atgaactaca tctgccgtag 4680 tttggaagaa caatacggta tgccttggat ggagttcaac ttcttcggcc ccaccaagat 4740 tgctgcttct ttacgtgaaa tcgcagctaa gtttgattct aagattcaag aaaacgctga 4800 gaaggtaatt gctaagtaca caccagtaat gaatgctgta ctagataaat accgccctcg 4860 cttggaaggt aacaccgtaa tgttgtacgt aggtggtcta cgtcctcgcc acgttgttcc 4920 cgcttttgaa gacctgggta tcaaagtagt tggtactggc tatgaattcg ctcacaatga 4980 cgattacaaa cgtaccaccc actacatcga taacgccacc atcatttacg atgacgttac 5040 cgcctacgaa tttgaagagt tcgtaaaagc taagaagcct gatttaattg cttctggtat 5100 taaagagaag tacgtcttcc aaaagatggg tcttcccttc cgtcaaatgc actcttggga 5160 ttactccggc ccttatcacg gttacgacgg attcgctatc ttcgcccgtg acatggattt 5220 agccctcaac agcccaactt ggagcttgat tggcgctcct tggaagaaag cggctgcaaa 5280 ggctaaggct gctgcctaac tcgagagata caacaatgcc tcagaatcca gaaagaactg 5340 tagaccacgt tgatctattc aaacagccag aatacaccga gctatttgaa aacaagagaa 5400 agaactttga aggcgctcat cctcctgaag aagttgaaag agtgtctgaa tggacaaaat 5460 cttgggacta ccgggaaaag aacttcgctc gtgaagcttt aaccgttaac cctgctaaag 5520 gttgccaacc tgtaggcgcg atgttcgctg ctttgggttt tgaaggtact ctacctttcg 5580 tacaaggttc tcaaggttgc gttgcttact tccgtacaca cctcagccgt cactacaaag 5640 agccttgctc cgcagtatct tcttccatga cagaagatgc agcagtattc ggtggtttga 5700 acaacatgat cgaaggtatg caggtttcat accaactgta caagcctaag atgattgctg 5760 tttgcaccac ctgtatggcg gaagttatcg gagatgactt gggcgcgttc atcaccaact 5820 ccaagaacgc tggttctatt cctcaagatt tccccgtacc ctttgctcac acacctagct 5880 ttgttggttc ccacatcact ggctacgaca acatgatgaa gggtattctg tctaacttga 5940 cagaaggtaa gaagaaagct accagcaacg gcaaaattaa cttcattcct ggttttgata 6000 cctatgtagg taacaaccgc gaattgaagc ggatgatggg tgtaatgggt gttgactaca 6060 ccatcctgtc tgacagcagc gactactttg attcacctaa catgggtgaa tacgaaatgt 6120 acccaggtgg tacaaagctg gaagatgcgg ctgattctat caacgctaaa gcaactgttg 6180 ctctccaagc ttacaccaca cctaagaccc gcgaatacat caaaacccag tggaagcaag 6240 aaacacaagt attgcgcccc ttcggtgtta agggtactga cgagttcttg actgctgttt 6300 ctgaattgac cggtaaagct attcctgaag aattggaaat cgaacgcggt cgtttagttg 6360 atgctatcac tgactcctac gcttggattc atggtaagaa gttcgctatc tacggcgatc 6420 cagatttgat catctccatc accagcttct tgttagaaat gggtgctgaa ccagtacaca 6480 tcctctgcaa caacggtgat gacaccttca agaaagaaat ggaagctatc ctcgctgcta 6540 gcccatttgg taaagaagct aaagtctgga ttcaaaaaga cttgtggcac ttccgttcct 6600 tgttgttcac cgagcctgta gacttcttca tcggtaactc ctacggtaag tacctgtggc 6660 gcgataccag catcccaatg gtgcggattg gttatcctct cttcgatcgc caccacttac 6720 accgctattc taccctcggc taccaaggtg gtctaaatat cctcaactgg gttgttaaca 6780 ccctgttgga tgaaatggat cgcagcacca acatcactgg taagaccgat atctcctttg 6840 acttgatccg ctaggcggcc gctcgctacc ttaggaccgt tatagttatt accctgttat 6900 ccctaaccgg tggaggcttc ttgttatgac atgttttttt ggggtacagt ctatgcctcg 6960 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa 7020 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt 7080 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga 7140 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca 7200 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc 7260 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc 7320 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg 7380 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc 7440 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt 7500 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc 7560 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa 7620 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa 7680 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact 7740 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca 7800 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatc 7860 tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccacctgatc 7920 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca 7980 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat 8040 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat 8100 aaaatttcta attcctaaaa ccaaaatcca gtgacgcggc cgcattaccc tgttatccct 8160 attaattaag agctcgctac cttaagagag gatatcggcg cgccgatcct agcctagtct 8220 ataggaggtt ttgaaaagaa aggagcaata atcattttct tgttctgtca agagggtgct 8280 attgctcctt tctttttttt tttttcttta ctaatttcct agtattttac tgacatagac 8340 ttttttgttt acattatcga aaaagaaaga gagggtattt gcttgcattt attcatgatg 8400 gatcccccgg gctgcaggca tgcaagctaa ttcccgatct agtaacatag atgacaccgc 8460 gcgccgcgga actagtaatt aattcccgcc tttcgctttt tgggggtgga aggcaaaaga 8520 aaacgtaggg gagggataga atcactacac tatcacggcc aactatacca actccttaat 8580 gtaaggatat atttaatgct atttatgaaa ttcaataata aaaagaaata gtaaaaaaat 8640 tactttatct tggatcttgg gcggatagcg ggaatcgaac ccgcatcttc tccttggcaa 8700 agagaaattt taccattcga ccatatccgc atttttttgt tcttgataca caatatgtac 8760 ccacatatat gatatataac cggatcttat ttgtgcagtg ccgggacaca tattctcttc 8820 ggaacgattc caataatttt gttaattata ttctttttat tcaagaagtt tgacccccct 8880 ctaatttttt tgttttcttt atttgatttg cattttcttt ggggacttag attcaaattt 8940 aatgtgtctc acaaccgaga aaaattaggg gggtcatttt ggttttgggt ctgcgacgaa 9000 taggttcaag agatgagaga attaaggata cccaccagaa agactaatcc aatccataag 9060 gaggtaccag aaaatacaac atttttgtta cttgaccagc catcaggaga agcaaataca 9120 acgggtacgc taatcaataa gattaatgaa gtagcaatta atgcaaaaac agccaattgg 9180 aaagcaagag tcatgctttt aatcctccaa gctaccaaca aatgaactat ataccatttg 9240 atccctctat cagccaaaaa atattaattg tgataaaata tgtcatcgag ggattttact 9300 ttatcatgaa tccattgatt ctatatgact tattactact ccccctttcg cactttattc 9360 gtacatggag tggggtgggg ggaaatggaa ttttcttttt tatttcacaa atggacatgc 9420 tagatcatat atctatatac ggatagatag atcgatcggc ggattcgcac ctgagatctt 9480 tctacagata gtgggggtat ccacccctat agccatgttc tattcggagg aataaaataa 9540 aaatagtctt tcggagagat ggctgagtgg ttgatagccc cggtcttgaa aaccggtata 9600 gttttgaaca aagaactatc gagggttcga atccctctct ctcctttttt gctaattgaa 9660 tagatttttt tatttagtgg ttttgcccaa tctgctatcc gaaagaaaag ggaatggctc 9720 ggctatccca cctagccaag ccagaaaaat agattagata taaattagat aaaataaatg 9780 agttgaaaaa aaaaaaagaa aaaaggaata cttaagctga ttccaagatg tatgattgaa 9840 tcaaagtaat ttgtacttca ttcaagcatt ggatctcctg tctcatatca attaagaggg 9900 gtcatggaaa gaacaggttc aaagtcgcga tcaattcctt tttcaaatcc tgctgcagcg 9960 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca 10020 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 10080 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 10140 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 10200 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 10260 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 10320 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 10380 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 10440 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 10500 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 10560 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 10620 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 10680 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 10740 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 10800 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 10860 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 10920 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 10980 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 11040 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 11100 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 11160 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 11220 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 11280 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 11340 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 11400 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 11460 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 11520 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 11580 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 11640 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 11700 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 11760 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 11820 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 11880 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 11940 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 12000 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 12060 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 12120 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac 12166 <210> 2 <211> 12166 <212> DNA <213> Escherichia coli <400> 2 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 60 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 120 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 180 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 240 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 300 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 360 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 420 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg 660 attggggcgt ggacataagg gtctttatga cacaatcaac aattcgcttc attttcaatt 720 aggccttgct ctagcttctt taggggttat tacttctttg gtagctcaac acatgtactc 780 tttacctgct tatgcattca tagcacaaga ctttactact caagctgcat tatataccca 840 ccaccaatat atcgcaggat tcatcatgac aggagctttt gctcatggag ctatattttt 900 cattagagat tacaatccgg agcaaaatga agataatgta ttggcaagaa tgttagatca 960 taaagaagct atcatatctc atttaagttg ggccagcctc tttctgggat tccataccct 1020 gggactttat gttcataatg atgtcatgct tgcctttggc actccggaga agcaaatctt 1080 gattgaaccg atatttgctc aatggataca atccgctcat ggtaaaactt catatgggtt 1140 cgatgtactt ttatcttcaa cgactggccc agcattcaat gcgggtcgaa gcatctggtt 1200 gccgggttgg ttaaatgctg ttaatgaaaa tagtaattca ttattcttaa caataggtcc 1260 tggagacttt ttggttcatc atgctattgc ccttggttta catacaacta cattgatctt 1320 agtaaaaggt gctttagatg cacgtggttc caagttaatg ccagataaaa aggatttcgg 1380 ttatagtttt ccgtgcgatg gcccaggacg aggcggtact tgtgatattt cggcatggga 1440 cgcgttttat ttggcagttt tttggatgtt aaatactatt ggatgggtta ctttttattg 1500 gcattggaag cacatcacat tatggcaagg taacgtttca cagtttaatg aatcttccac 1560 ttatttgatg ggctggttaa gggattattt atggttaaac tcttcacaac ttatcaatgg 1620 atataaccct tttggtatga atagtttatc ggtttgggca tggatgttct tatttggaca 1680 tcttgtttgg gctactggat ttatgttctt aatttcttgg cgtggatatt ggcaggaatt 1740 gattgaaact ttagcatggg ctcacgaacg cacacctttg gccaatttga ttcgatggag 1800 agataaacca gtggcccttt ctattgtaca agcaagattg gttggattag ctcacttttc 1860 tgtaggttat atattcactt atgcggcttt cttgattgcc tctacgtcgg gcaaatttgg 1920 ttaattaatg tgtgtattcg cgataatctc atttctttcg acggagaagg gggtccacct 1980 tcttctattt ctacatctag gattcgactt gtatcatgga tactaatagg aattcaacca 2040 ttatggcaag gaaaagtttg attcagaggg agaagaagag gcaaaaattg gaacagaaat 2100 atcattcgat tcgtcgatcc tcaaaaaaag aaataagcaa ggttccgtcg ttgagtgaca 2160 aatgggaaat ttatggaaag ttacaatccc taccacggaa tagtgcacct acacgccttc 2220 atcgacgttg ttttttgacc ggaaggccga gagctaacta tcgagacttt ggcctatccg 2280 gacacatact tcgtgaaatg gttcatgcat gtttgttgcc aggagcaaca agatcaagtt 2340 ggtaaggatt aacgcttcat ttctatttct atggtcgatg atcatagaag cccctttacc 2400 attctgtata aatgggctat tctatttgta cagatagggt ggaggggcgc atttaatcct 2460 tgtttatcta ttagttttca gttcttatct ttggcgcggg gtagagcagt ttggtagctc 2520 gcaaggctca taaccttgag gtcacgggtt caaatcctgt ctccgcaaca tcttattctg 2580 gtctcgaggt ccgcataaag aaccacccat aatacccata atagctgttt gccaaccggt 2640 cgccgtcgtt caatgagaat ggataagagg ctcgtgggat tgacgtgagg gggcagggat 2700 gactatattt ctgggagcga actccgggcg aatatgaagc gcatggatac aagttatgcc 2760 ttggaatgaa agacaattcc gaatccgctt tgtctacgaa caaggaagct ataagtaatg 2820 caactatgaa ggatctatga ctgatgaaaa tattagacaa attgcttttt atggaaaagg 2880 aggaattgga aaatctacta cttctcaaaa tactttagct gctatggctg aaatgggaca 2940 aagaattatg attgtaggat gtgatcctaa agctgattct actagattaa tgttacattc 3000 taaagctcaa actactgtat tacatttagc tgctgaaaga ggagctgtag aagatttaga 3060 attacatgaa gtaatgttaa ctggatttag aggagtaaaa tgtgtagaat ctggaggacc 3120 tgaacctgga gtaggatgtg ctggaagagg aattattact gctattaatt ttttagaaga 3180 aaatggagct tatcaagatt tagattttgt atcttatgat gtattaggag atgtagtatg 3240 tggaggattt gctatgccta ttagagaagg aaaagctcaa gaaatttata ttgtaacttc 3300 tggagaaatg atggctatgt atgctgctaa taatattgct agaggaattt taaaatatgc 3360 tcattctgga ggagtaagat taggaggatt aatttgtaat tctagaaaag tagatagaga 3420 agatgaatta attatgaatt tagctgaaag attaaatact caaatgattc attttgtacc 3480 tagagataat attgtacaac atgctgaatt aagaagaatg actgtaaatg aatatgctcc 3540 tgattctaat caaggacaag aatatagagc tttagctaaa aaaattatta ataatgataa 3600 attaactatt cctactccta tggaaatgga tgaattagaa gctttattaa ttgaatatgg 3660 attattagat gatgatacta aacattctga aattattgga aaacctgctg aagctactaa 3720 atagcggccg caacccataa tacccataat agctgtttgc catcgctacc ttaggaccgt 3780 tatagttaac cggtggaggc agactatgac tcctcctgaa aataaaaatt tagtagatga 3840 aaataaagaa ttaattcaag aagtattaaa agcttatcct gaaaaatcta gaaaaaaaag 3900 agaaaaacat ttaaatgtac atgaagaaaa taaatctgat tgtggagtaa aatctaatat 3960 taaatctgta cctggagtaa tgactgctag aggatgtgct tatgctggat ctaaaggagt 4020 agtatgggga cctattaaag atatgattca tatttctcat ggacctgtag gatgtggata 4080 ttggtcttgg tctggaagaa gaaattatta tgtaggagta actggaatta attcttttgg 4140 aactatgcat tttacttctg attttcaaga aagagatatt gtatttggag gagataaaaa 4200 attaactaaa ttaattgaag aattagatgt attatttcct ttaaatagag gagtatctat 4260 tcaatctgaa tgtcctattg gattaattgg agatgatatt gaagctgtag ctaaaaaaac 4320 ttctaaacaa attggaaaac ctgtagtacc tttaagatgt gaaggattta gaggagtatc 4380 tcaatcttta ggacatcata ttgctaatga tgctattaga gattggattt ttcctgaata 4440 tgataaatta aaaaaagaaa atagattaga ttttgaacct tctccttatg atgtagcttt 4500 aattggagat tataatattg gaggagatgc ttgggcttct agaatgttat tagaagaaat 4560 gggattaaga gtagtagctc aatggtctgg agatggaact ttaaatgaat taattcaagg 4620 acctgctgct aaattagtat taattcattg ttatagatct atgaattata tttgtagatc 4680 tttagaagaa caatatggaa tgccttggat ggaatttaat ttttttggac ctactaaaat 4740 tgctgcttct ttaagagaaa ttgctgctaa atttgattct aaaattcaag aaaatgctga 4800 aaaagtaatt gctaaatata ctcctgtaat gaatgctgta ttagataaat atagacctag 4860 attagaagga aatactgtaa tgttatatgt aggaggatta agacctagac atgtagtacc 4920 tgcttttgaa gatttaggaa ttaaagtagt aggaactgga tatgaatttg ctcataatga 4980 tgattataaa agaactactc attatattga taatgctact attatttatg atgatgtaac 5040 tgcttatgaa tttgaagaat ttgtaaaagc taaaaaacct gatttaattg cttctggaat 5100 taaagaaaaa tatgtatttc aaaaaatggg attacctttt agacaaatgc attcttggga 5160 ttattctgga ccttatcatg gatatgatgg atttgctatt tttgctagag atatggattt 5220 agctttaaat tctcctactt ggtctttaat tggagctcct tggaaaaaag ctgctgctaa 5280 agctaaagct gctgcttaac tcgagagata caacaatgcc tcaaaatcct gaaagaactg 5340 tagatcatgt agatttattt aaacaacctg aatatactga attatttgaa aataaaagaa 5400 aaaattttga aggagctcat cctcctgaag aagtagaaag agtatctgaa tggactaaat 5460 cttgggatta tagagaaaaa aattttgcta gagaagcttt aactgtaaat cctgctaaag 5520 gatgtcaacc tgtaggagct atgtttgctg ctttaggatt tgaaggaact ttaccttttg 5580 tacaaggatc tcaaggatgt gtagcttatt ttagaactca tttatctaga cattataaag 5640 aaccttgttc tgctgtatct tcttctatga ctgaagatgc tgctgtattt ggaggattaa 5700 ataatatgat tgaaggaatg caagtatctt atcaattata taaacctaaa atgattgctg 5760 tatgtactac ttgtatggct gaagtaattg gagatgattt aggagctttt attactaatt 5820 ctaaaaatgc tggatctatt cctcaagatt ttcctgtacc ttttgctcat actccttctt 5880 ttgtaggatc tcatattact ggatatgata atatgatgaa aggaatttta tctaatttaa 5940 ctgaaggaaa aaaaaaagct acttctaatg gaaaaattaa ttttattcct ggatttgata 6000 cttatgtagg aaataataga gaattaaaaa gaatgatggg agtaatggga gtagattata 6060 ctattttatc tgattcttct gattattttg attctcctaa tatgggagaa tatgaaatgt 6120 atcctggagg aactaaatta gaagatgctg ctgattctat taatgctaaa gctactgtag 6180 ctttacaagc ttatactact cctaaaacta gagaatatat taaaactcaa tggaaacaag 6240 aaactcaagt attaagacct tttggagtaa aaggaactga tgaattttta actgctgtat 6300 ctgaattaac tggaaaagct attcctgaag aattagaaat tgaaagagga agattagtag 6360 atgctattac tgattcttat gcttggattc atggaaaaaa atttgctatt tatggagatc 6420 ctgatttaat tatttctatt acttcttttt tattagaaat gggagctgaa cctgtacata 6480 ttttatgtaa taatggagat gatactttta aaaaagaaat ggaagctatt ttagctgctt 6540 ctccttttgg aaaagaagct aaagtatgga ttcaaaaaga tttatggcat tttagatctt 6600 tattatttac tgaacctgta gattttttta ttggaaattc ttatggaaaa tatttatgga 6660 gagatacttc tattcctatg gtaagaattg gatatccttt atttgataga catcatttac 6720 atagatattc tactttagga tatcaaggag gattaaatat tttaaattgg gtagtaaata 6780 ctttattaga tgaaatggat agatctacta atattactgg aaaaactgat atttcttttg 6840 atttaattag ataggcggcc gctcgctacc ttaggaccgt tatagttatt accctgttat 6900 ccctaaccgg tggaggcttc ttgttatgac atgttttttt ggggtacagt ctatgcctcg 6960 ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa 7020 cgatgttacg cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt 7080 gatcgccgaa gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga 7140 accgacgttg ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca 7200 cagtgatatt gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc 7260 tttgatcaac gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc 7320 tgtagaagtc accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg 7380 cgaactgcaa tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc 7440 cacgatcgac attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt 7500 ggtaggtcca gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc 7560 gctaaatgaa accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa 7620 tgtagtgctt acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa 7680 ggatgtcgct gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact 7740 tgaagctaga caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca 7800 gttggaagaa tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatc 7860 tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat ccacctgatc 7920 tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta tttttctcca 7980 gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg tttcgctcat 8040 gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac ttctatcaat 8100 aaaatttcta attcctaaaa ccaaaatcca gtgacgcggc cgcattaccc tgttatccct 8160 attaattaag agctcgctac cttaagagag gatatcggcg cgccgatcct agcctagtct 8220 ataggaggtt ttgaaaagaa aggagcaata atcattttct tgttctgtca agagggtgct 8280 attgctcctt tctttttttt tttttcttta ctaatttcct agtattttac tgacatagac 8340 ttttttgttt acattatcga aaaagaaaga gagggtattt gcttgcattt attcatgatg 8400 gatcccccgg gctgcaggca tgcaagctaa ttcccgatct agtaacatag atgacaccgc 8460 gcgccgcgga actagtaatt aattcccgcc tttcgctttt tgggggtgga aggcaaaaga 8520 aaacgtaggg gagggataga atcactacac tatcacggcc aactatacca actccttaat 8580 gtaaggatat atttaatgct atttatgaaa ttcaataata aaaagaaata gtaaaaaaat 8640 tactttatct tggatcttgg gcggatagcg ggaatcgaac ccgcatcttc tccttggcaa 8700 agagaaattt taccattcga ccatatccgc atttttttgt tcttgataca caatatgtac 8760 ccacatatat gatatataac cggatcttat ttgtgcagtg ccgggacaca tattctcttc 8820 ggaacgattc caataatttt gttaattata ttctttttat tcaagaagtt tgacccccct 8880 ctaatttttt tgttttcttt atttgatttg cattttcttt ggggacttag attcaaattt 8940 aatgtgtctc acaaccgaga aaaattaggg gggtcatttt ggttttgggt ctgcgacgaa 9000 taggttcaag agatgagaga attaaggata cccaccagaa agactaatcc aatccataag 9060 gaggtaccag aaaatacaac atttttgtta cttgaccagc catcaggaga agcaaataca 9120 acgggtacgc taatcaataa gattaatgaa gtagcaatta atgcaaaaac agccaattgg 9180 aaagcaagag tcatgctttt aatcctccaa gctaccaaca aatgaactat ataccatttg 9240 atccctctat cagccaaaaa atattaattg tgataaaata tgtcatcgag ggattttact 9300 ttatcatgaa tccattgatt ctatatgact tattactact ccccctttcg cactttattc 9360 gtacatggag tggggtgggg ggaaatggaa ttttcttttt tatttcacaa atggacatgc 9420 tagatcatat atctatatac ggatagatag atcgatcggc ggattcgcac ctgagatctt 9480 tctacagata gtgggggtat ccacccctat agccatgttc tattcggagg aataaaataa 9540 aaatagtctt tcggagagat ggctgagtgg ttgatagccc cggtcttgaa aaccggtata 9600 gttttgaaca aagaactatc gagggttcga atccctctct ctcctttttt gctaattgaa 9660 tagatttttt tatttagtgg ttttgcccaa tctgctatcc gaaagaaaag ggaatggctc 9720 ggctatccca cctagccaag ccagaaaaat agattagata taaattagat aaaataaatg 9780 agttgaaaaa aaaaaaagaa aaaaggaata cttaagctga ttccaagatg tatgattgaa 9840 tcaaagtaat ttgtacttca ttcaagcatt ggatctcctg tctcatatca attaagaggg 9900 gtcatggaaa gaacaggttc aaagtcgcga tcaattcctt tttcaaatcc tgctgcagcg 9960 agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca 10020 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga 10080 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg 10140 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 10200 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac 10260 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata 10320 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa 10380 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct 10440 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa 10500 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg 10560 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca 10620 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa 10680 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg 10740 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg 10800 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg 10860 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc 10920 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag 10980 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac 11040 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc 11100 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag 11160 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt 11220 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag 11280 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca 11340 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact 11400 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca 11460 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg 11520 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc 11580 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg 11640 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca 11700 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt 11760 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc 11820 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc 11880 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca 11940 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa 12000 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat 12060 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa 12120 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac 12166 <210> 3 <211> 11875 <212> DNA <213> Escherichia coli <400> 3 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 60 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 120 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 180 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 240 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 300 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 360 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 420 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccat 660 atttaatatg gcatcaccag aggtaccaga aatgtattta cctttcctcc caaaaacact 720 tattttaaaa tctaattatt gacagaagtt gaccttgtaa atattactta aattaattta 780 gcagtatgtc aatttagtta gttatagtac cactttggga gagggtatta cattaattta 840 ttttgaaatt tacttatagt aaatagagat ttgggaacaa caactgctag cctaccgatt 900 tacaaaacgt ggttttaata atttattaat tggaaggcga ctgcaaaaga atattttgca 960 gcataggatt tatttctaat ttttctaaag ttaccaattg agttaaaaaa agctttctta 1020 attcaacatt tttaagtaaa tactgtttaa tgttatactt ttacgaatac acatatggta 1080 aaaaataaaa caatatcttt aaaataagta aaaataattt gtaaaccaat aaaaaatata 1140 tttatggtat aatataacat atgatgtaaa aaaaactatt tgtctaattt aataaccatg 1200 cattttttat gaacacataa taattaaaag cgttgctaat ggtgtaaata atgtatttat 1260 taaattaaat aattgttatt ataaggagaa atccatgaca attagtactc cagagcgcga 1320 agcgaaaaaa gtaaagattg cggttgatcg taatcctgta gaaacaagtt ttgaaaaatg 1380 ggcgtgcgac ctgaaataag gatttgacta attaaatata aaaattttta actaaaataa 1440 gctgaaaaag cttattttag ttttaagcac tgttggccga gcggatgagg caaacgactc 1500 ataatcgtta taaggtaggt tcaactccta tacggtgcaa ctaagtaaag aataagcaat 1560 acttcctttt caaaatatgg atggtcataa tatatgctat tctggtagaa tttataacaa 1620 gccttttcag aagatatata tatataactt tttatagtaa acttgttgga gatttaaatt 1680 aacatctagg agcaatcgtc cactctaatg agttaatata actctaaaag ctgaaagtaa 1740 tccatattgt tcctactccg gttttctaag ttatttttgt taaaggagtc gtctggttta 1800 ctccaacaaa agaaattggt tttatttttt ctatgtcata tacatgcgat agagtattag 1860 tttttaatac ttgcttaaaa aaaatttttg tgatataata ttattagatt aagttaatgc 1920 ggacatagct caatggtaga gtatttcctt gccaaggaaa atgttgcggg ttcgactccc 1980 gttgtccgct agaaactact cgaggtccgc ataaagaacc acccataata cccataatag 2040 ctgtttgcca accggtcgcg cctgccaact gcctatattt atatactgcg ataaacttta 2100 gtcccgaagg ggtttacata tccgaaggag gaagcaggca gtggcggtac cacgccactg 2160 gcgtcctaat ataaatattg ggcaagtaaa cttagaataa aatttatttg ctgcgttagc 2220 aggtttacat actcctaagt ttacttgccc gaaggggaag gaggacgtcc cctacgggaa 2280 tataaatatt agtggcagtg gtacaataaa taaattgtat gtaaacccct tcgggcaact 2340 aaagtttatc gcagtattaa catcctagta tataaatatc ggcagttggc aggcaacaaa 2400 tttatttatt gtcccgtaag gggaagggga aaacaattat tattttactg cggagcagct 2460 tgttattaga aatttttatt aaaaaaaaaa taaaaatttg acaaaaaaaa ataaaaaagt 2520 taaattaaaa acactgggaa tgttctaaca atcataaaaa aatcaaaagg gtttaaaatc 2580 ccgacaaaat ttaaacttta aagagtatga cagatgaaaa tattcgtcaa attgcttttt 2640 atggtaaagg tggtattggt aaatcaacaa catcacaaaa tacattagct gctatggctg 2700 aaatgggtca acgtattatg attgttggtt gtgatccaaa agctgattca acacgtttaa 2760 tgttacattc aaaagctcaa acaacagttt tacatttagc tgctgaacgt ggtgctgttg 2820 aagatttaga attacatgaa gttatgttaa caggttttcg tggtgttaaa tgtgttgaat 2880 caggtggtcc agaaccaggt gttggttgtg ctggtcgtgg tattattaca gctattaatt 2940 ttttagaaga aaatggtgct tatcaagatt tagattttgt ttcatatgat gttttaggtg 3000 atgttgtttg tggtggtttt gctatgccaa ttcgtgaagg taaagctcaa gaaatttata 3060 ttgttacatc aggtgaaatg atggctatgt atgctgctaa taatattgct cgtggtattt 3120 taaaatatgc tcattcaggt ggtgttcgtt taggtggttt aatttgtaat tcacgtaaag 3180 ttgatcgtga agatgaatta attatgaatt tagctgaacg tttaaataca caaatgattc 3240 attttgttcc acgtgataat attgttcaac atgctgaatt acgtcgtatg acagttaatg 3300 aatatgctcc agattcaaat caaggtcaag aatatcgtgc tttagctaaa aaaattatta 3360 ataatgataa attaacaatt ccaacaccaa tggaaatgga tgaattagaa gctttattaa 3420 ttgaatatgg tttattagat gatgatacaa aacattcaga aattattggt aaaccagctg 3480 aagctacaaa atagcggccg caacccataa tacccataat agctgtttgc catcgctacc 3540 ttaggaccgt tatagttaac cggtggaggc agactatgac accaccagaa aataaaaatt 3600 tagttgatga aaataaagaa ttaattcaag aagttttaaa agcttatcca gaaaaatcac 3660 gtaaaaaacg tgaaaaacat ttaaatgttc atgaagaaaa taaatcagat tgtggtgtta 3720 aatcaaatat taaatcagtt ccaggtgtta tgacagctcg tggttgtgct tatgctggtt 3780 caaaaggtgt tgtttggggt ccaattaaag atatgattca tatttcacat ggtccagttg 3840 gttgtggtta ttggtcatgg tcaggtcgtc gtaattatta tgttggtgtt acaggtatta 3900 attcatttgg tacaatgcat tttacatcag attttcaaga acgtgatatt gtttttggtg 3960 gtgataaaaa attaacaaaa ttaattgaag aattagatgt tttatttcca ttaaatcgtg 4020 gtgtttcaat tcaatcagaa tgtccaattg gtttaattgg tgatgatatt gaagctgttg 4080 ctaaaaaaac atcaaaacaa attggtaaac cagttgttcc attacgttgt gaaggttttc 4140 gtggtgtttc acaatcatta ggtcatcata ttgctaatga tgctattcgt gattggattt 4200 ttccagaata tgataaatta aaaaaagaaa atcgtttaga ttttgaacca tcaccatatg 4260 atgttgcttt aattggtgat tataatattg gtggtgatgc ttgggcttca cgtatgttat 4320 tagaagaaat gggtttacgt gttgttgctc aatggtcagg tgatggtaca ttaaatgaat 4380 taattcaagg tccagctgct aaattagttt taattcattg ttatcgttca atgaattata 4440 tttgtcgttc attagaagaa caatatggta tgccatggat ggaatttaat ttttttggtc 4500 caacaaaaat tgctgcttca ttacgtgaaa ttgctgctaa atttgattca aaaattcaag 4560 aaaatgctga aaaagttatt gctaaatata caccagttat gaatgctgtt ttagataaat 4620 atcgtccacg tttagaaggt aatacagtta tgttatatgt tggtggttta cgtccacgtc 4680 atgttgttcc agcttttgaa gatttaggta ttaaagttgt tggtacaggt tatgaatttg 4740 ctcataatga tgattataaa cgtacaacac attatattga taatgctaca attatttatg 4800 atgatgttac agcttatgaa tttgaagaat ttgttaaagc taaaaaacca gatttaattg 4860 cttcaggtat taaagaaaaa tatgtttttc aaaaaatggg tttaccattt cgtcaaatgc 4920 attcatggga ttattcaggt ccatatcatg gttatgatgg ttttgctatt tttgctcgtg 4980 atatggattt agctttaaat tcaccaacat ggtcattaat tggtgctcca tggaaaaaag 5040 ctgctgctaa agctaaagct gctgcttaac tcgagagata caacaatgcc acaaaatcca 5100 gaacgtacag ttgatcatgt tgatttattt aaacaaccag aatatacaga attatttgaa 5160 aataaacgta aaaattttga aggtgctcat ccaccagaag aagttgaacg tgtttcagaa 5220 tggacaaaat catgggatta tcgtgaaaaa aattttgctc gtgaagcttt aacagttaat 5280 ccagctaaag gttgtcaacc agttggtgct atgtttgctg ctttaggttt tgaaggtaca 5340 ttaccatttg ttcaaggttc acaaggttgt gttgcttatt ttcgtacaca tttatcacgt 5400 cattataaag aaccatgttc agctgtttca tcatcaatga cagaagatgc tgctgttttt 5460 ggtggtttaa ataatatgat tgaaggtatg caagtttcat atcaattata taaaccaaaa 5520 atgattgctg tttgtacaac atgtatggct gaagttattg gtgatgattt aggtgctttt 5580 attacaaatt caaaaaatgc tggttcaatt ccacaagatt ttccagttcc atttgctcat 5640 acaccatcat ttgttggttc acatattaca ggttatgata atatgatgaa aggtatttta 5700 tcaaatttaa cagaaggtaa aaaaaaagct acatcaaatg gtaaaattaa ttttattcca 5760 ggttttgata catatgttgg taataatcgt gaattaaaac gtatgatggg tgttatgggt 5820 gttgattata caattttatc agattcatca gattattttg attcaccaaa tatgggtgaa 5880 tatgaaatgt atccaggtgg tacaaaatta gaagatgctg ctgattcaat taatgctaaa 5940 gctacagttg ctttacaagc ttatacaaca ccaaaaacac gtgaatatat taaaacacaa 6000 tggaaacaag aaacacaagt tttacgtcca tttggtgtta aaggtacaga tgaattttta 6060 acagctgttt cagaattaac aggtaaagct attccagaag aattagaaat tgaacgtggt 6120 cgtttagttg atgctattac agattcatat gcttggattc atggtaaaaa atttgctatt 6180 tatggtgatc cagatttaat tatttcaatt acatcatttt tattagaaat gggtgctgaa 6240 ccagttcata ttttatgtaa taatggtgat gatacattta aaaaagaaat ggaagctatt 6300 ttagctgctt caccatttgg taaagaagct aaagtttgga ttcaaaaaga tttatggcat 6360 tttcgttcat tattatttac agaaccagtt gattttttta ttggtaattc atatggtaaa 6420 tatttatggc gtgatacatc aattccaatg gttcgtattg gttatccatt atttgatcgt 6480 catcatttac atcgttattc aacattaggt tatcaaggtg gtttaaatat tttaaattgg 6540 gttgttaata cattattaga tgaaatggat cgttcaacaa atattacagg taaaacagat 6600 atttcatttg atttaattcg ttaggcggcc gctcgctacc ttaggaccgt tatagttatt 6660 accctgttat ccctaaccgg tggaggcttc ttgttatgac atgttttttt ggtgttcaat 6720 caatgccacg tgcttcaaaa caacaagctc gttatgctgt tggtcgttgt ttaatgttat 6780 ggtcatcaaa tgatgttaca caacaaggtt cacgtccaaa aacaaaatta aatattatgg 6840 gtgaagctgt tattgctgaa gtttcaacac aattatcaga agttgttggt gttattgaac 6900 gtcatttaga accaacatta ttagctgttc atttatatgg ttcagctgtt gatggtggtt 6960 taaaaccaca ttcagatatt gatttattag ttacagttac agttcgttta gatgaaacaa 7020 cacgtcgtgc tttaattaat gatttattag aaacatcagc ttcaccaggt gaatcagaaa 7080 ttttacgtgc tgttgaagtt acaattgttg ttcatgatga tattattcca tggcgttatc 7140 cagctaaacg tgaattacaa tttggtgaat ggcaacgtaa tgatatttta gctggtattt 7200 ttgaaccagc tacaattgat attgatttag ctattttatt aacaaaagct cgtgaacatt 7260 cagttgcttt agttggtcca gctgctgaag aattatttga tccagttcca gaacaagatt 7320 tatttgaagc tttaaatgaa acattaacat tatggaattc accaccagat tgggctggtg 7380 atgaacgtaa tgttgtttta acattatcac gtatttggta ttcagctgtt acaggtaaaa 7440 ttgctccaaa agatgttgct gctgattggg ctatggaacg tttaccagct caatatcaac 7500 cagttatttt agaagctcgt caagcttatt taggtcaaga agaagatcgt ttagcttcac 7560 gtgctgatca attagaagaa tttgttcatt atgttaaagg tgaaattaca aaagttgttg 7620 gtaaataatc tcgagctcaa gcttcgaatt ctgcagtcga cggtaccgcg ggcccgggat 7680 ccacctgatc tagagtccgc aaaaatcacc agtctctctc tacaaatcta tctctctcta 7740 tttttctcca gaataatgtg tgagtagttc ccagataagg gaattagggt tcttataggg 7800 tttcgctcat gtgttgagca tataagaaac ccttagtatg tatttgtatt tgtaaaatac 7860 ttctatcaat aaaatttcta attcctaaaa ccaaaatcca gtgacgcggc cgcattaccc 7920 tgttatccct attaattaag agctcgctac cttaagagag gatatcggcg cgcctttttt 7980 tttaaactaa aataaatctg gttaaccata cctggtttat tttagtttat acacactttt 8040 catatatata tacttaatag ctaccatagg cagttggcag gacgtcccct tacgggacaa 8100 atgtatttat tgttgcctgc caactgccta atataaatat tagtggacgg atcccccggg 8160 ctgcaggcat gcaagctaat tcccgatcta gtaacataga tgacaccgcg cgccgcggaa 8220 gaatagaagt tacaacgtaa ttctacgttg ggttaattta ttaacccctt aaaggggttg 8280 catttagaat ccaggcatct tgggtaaatt atgtataata aaactatatt aaaagttaat 8340 tcattaaagc cgtttattta aaataaataa aaaattatgt cacgttattt aggccctcgt 8400 ttgagagtta ttcgtcgtat tggtaaatta agaggtttta cgcgtaaaaa acctttccgt 8460 cgtgtattta aaggttttgg tggttttaaa ggtaaagtta ttcctccagg tcaacatggt 8520 ttaacaaaac tattaaaaac aagaccatat gattcatctg aatcggatta tctaatccgt 8580 ttaaaagtaa aacaacgttt acgttttaac tatggtatta ctgaacgtca acttgttaac 8640 tatgtacgta aagctaaaaa aattaaagaa tctacaggtc aagttttact acaattttta 8700 gaaatgcgtt tagataatat tgtattccgt ttaaatatgg cacctacaat tccagcagca 8760 cgccaactta ttagtcatgg acatattcgt gtaaataata aaaaagtaaa tattcctagc 8820 tatatgtgta aaccaaaaga tgttatttct gtagcaatga aacaacgttc gttacaactt 8880 gtaaataaaa acttacaaga atattaccgt cgtatgcgtt tctataaaaa acgtttagaa 8940 aaaacattac cttttatttt actaaaaatt aaaccattag gtcttactag tgtaacagca 9000 gctgtagaac ttattactaa aggaaacgtg cgagtaaata ataagagtgt gaaaacgcca 9060 aattatattt gtcgtccacg agatacagtt tctttaagaa caaaacaagg tattaaaaaa 9120 gtatttttaa aaaattattt aaaaggttaa tttaatcggt aaaattccca ggtagttgca 9180 cctttccctt caggacgtcc ccttcccctt cggacaataa ataaatttgt tgcctgccaa 9240 caaatttatt tattgtatta aaatagaata aaatttattt gctgcggtag caggtttaca 9300 tacaatttat ttattgtacc actccactgg cgtccttcgg agtatgtaaa catgcttggc 9360 actggtttac atacaattta tttattgtac cactgccact gcgtcctcct tcggagtatg 9420 taaacatgct aagtttactt gcccaatatt tatattaggc agttggcagg caactcacta 9480 aaatttattt acccgaagga cgtcccgaag ggaaggggaa ggaggacgtc cccttacggg 9540 aatataaata ttagtggacg tcagtggcgg taccactgcc actggcgtcc tatatttata 9600 tactcctaag tttacttgcc caatatttat atacccgaag gaaggggaag catataaata 9660 tacttcggga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa 9720 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata 9780 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta 9840 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa 9900 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg 9960 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag 10020 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa 10080 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 10140 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 10200 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 10260 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 10320 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 10380 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 10440 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 10500 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 10560 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 10620 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 10680 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 10740 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 10800 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 10860 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 10920 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 10980 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 11040 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 11100 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 11160 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 11220 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 11280 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 11340 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 11400 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 11460 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 11520 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 11580 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 11640 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 11700 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 11760 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 11820 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac 11875 <210> 4 <211> 547 <212> DNA <213> Chlamydomonas reinhardtii <400> 4 gcctgccaac tgcctatatt tatatactgc gataaacttt agtcccgaag gggtttacat 60 atccgaagga ggaagcaggc agtggcggta ccacgccact ggcgtcctaa tataaatatt 120 gggcaagtaa acttagaata aaatttattt gctgcgttag caggtttaca tactcctaag 180 tttacttgcc cgaaggggaa ggaggacgtc ccctacggga atataaatat tagtggcagt 240 ggtacaataa ataaattgta tgtaaacccc ttcgggcaac taaagtttat cgcagtatta 300 acatcctagt atataaatat cggcagttgg caggcaacaa atttatttat tgtcccgtaa 360 ggggaagggg aaaacaatta ttattttact gcggagcagc ttgttattag aaatttttat 420 taaaaaaaaa ataaaaattt gacaaaaaaa aataaaaaag ttaaattaaa aacactggga 480 atgttctaac aatcataaaa aaatcaaaag ggtttaaaat cccgacaaaa tttaaacttt 540 aaagagt 547 <210> 5 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> TR-F, Adapter primer (SacI), DNA: Tomato <400> 5 aagagctcgc tgcagcagga tttgaaaaag ga 32 <210> 6 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> TR-R, Adapter primer (SacII), DNA: Tomato <400> 6 ttccgcggaa ctagtaatta attcccgcct ttcg 34 <210> 7 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> TL-F, Adapter primer (Xho1), DNA: Tomato <400> 7 aactcgagac cagaataaga tgttgcggag aca 33 <210> 8 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> TL-F, Adapter primer (Kpn1), DNA: Tomato <400> 8 aaaggtaccg attggggcgt ggacataagg 30 <210> 9 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> NifH atg Bgl11, Adapter primer (Bgl11), DNA: Cyanobacteria <400> 9 aaaagatcta tgactgacga aaacattaga caga 34 <210> 10 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> NifH Stop Not1, Adapter primer (Not1), DNA: Cyanobacteria <400> 10 tttgcggccg ctatttggta gcttctgcgg gct 33 <210> 11 <211> 37 <212> DNA <213> Artificial Sequence <220> <223> NifD ATG, Adapter primer (Age1), DNA:Cyanobacteria <400> 11 aaaccggtgg aggcagacta tgacacctcc tgaaaac 37 <210> 12 <211> 27 <212> DNA <213> Artificial Sequence <220> <223> NifD Stop, Adapter primer (XhoI), DNA: Cyanobacteria <400> 12 ttctcgagtt aggcagcagc cttagcc 27 <210> 13 <211> 34 <212> DNA <213> Artificial Sequence <220> <223> NifK ATG, Adapter primer (XhoI), DNA: Cyanobacteria <400> 13 aactcgagga gatacaacaa tgcctcagaa tcca 34 <210> 14 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> NifK Stop, Adapter primer (NotI), DNA: Cyanobacteria <400> 14 aaaagcggcc gctagcggat caagtcaaag gag 33 <210> 15 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> MCS F, Adapter primer (XhoI), DNA: pPZP-RCS <400> 15 aactcgagtc cgcataaaga accacccata a 31 <210> 16 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> MCS R, Adapter primer (SacII), DNA: pPZP-RCS <400> 16 tttccgcggc gcgcggtgtc atctatgtta cta 33 <210> 17 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> TPsbA F, Adapter primer (BamHI), DNA: Tomato <400> 17 tttggatcca tcatgaataa atgcaagcaa atacc 35 <210> 18 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> TPsbA R, Adapter primer (AscI), DNA: Tomato <400> 18 tttggcgcgc cgatcctagc ctagtctata ggaggttt 38 <210> 19 <211> 29 <212> DNA <213> Artificial Sequence <220> <223> Prrn165 R, Adapter Primer (BamHI) DNA: Tomato) <400> 19 ccaaccggtc gccgtcgttc aatgagaat 29 <210> 20 <211> 36 <212> DNA <213> Artificial Sequence <220> <223> Prrn16S R, Adapter primer (BamHI), DNA: Tomato <400> 20 tttggatcct tcatagttgc attacttata gcttcc 36 <210> 21 <211> 33 <212> DNA <213> Artificial Sequence <220> <223> NifH-20 ATG, Adapter primer (BglII), DNA: Cyanobacteria <400> 21 aaaagatcta ccaaccaatt gcaggaaaag aga 33 <210> 22 <211> 38 <212> DNA <213> Artificial Sequence <220> <223> NifH-6 ATG, Adapter primer (BglII), DNA: Cyanobacteria <400> 22 aaaagatcta ggaggagaac aatgactgac gaaaacat 38 <210> 23 <211> 35 <212> DNA <213> Artificial Sequence <220> <223> aada ATG, Adapter primer (AgeI), DNA: pPZP-RCS <400> 23 aaaccggtgg aggcttcttg ttatgacatg ttttt 35 <210> 24 <211> 32 <212> DNA <213> Artificial Sequence <220> <223> aada STOP, Adapter primer (XhoI), DNA: pPZP-RCS <400> 24 aactcgagat tatttgccga ctaccttggt ga 32 <210> 25 <211> 25 <212> DNA <213> Artificial Sequence <220> <223> Nif H, Adapter primer (N/A), DNA: Cyanobacteria <400> 25 atgactgacg aaaacattag acaga 25 <210> 26 <211> 30 <212> DNA <213> Artificial Sequence <220> <223> Derived from Chlorogloeopsos fritschii (NIF-H -K, #912) <400> 26 ctcgagatga ctgacgaaaa cattagacag 30 <210> 27 <211> 31 <212> DNA <213> Artificial Sequence <220> <223> Nif-H XbaI, Adapter primer (XbaI), DNA: Cyanobacteria <400> 27 tctagaatga ctgacgaaaa cattagacag a 31 <210> 28 <211> 193 <212> DNA <213> Solanum lycopersicum <400> 28 cgtcgttcaa tgagaatgga taagaggctc gtgggattga cgtgaggggg cagggatgac 60 tatatttctg ggagcgaact ccgggcgaat atgaagcgca tggatacaag ttatgccttg 120 gaatgaaaga caattccgaa tccgctttgt ctacgaacaa ggaagctata agtaatgcaa 180 ctatgaagga tct 193 <210> 29 <211> 984 <212> DNA <213> Artificial Sequence <220> <223> Derived from Cyanothece ATCC51142 (NIF-H; #42) <400> 29 atgggccgca acagccaggg cttcctgacg acgatccgca tcatcgtcac gagcgcgacg 60 gaccagccga gcaacttcat ccacagcctg agcaacaagc gcgagagcac gatgcgccag 120 atcgcgttct acggcaaggg cggcatcggc aagagcacga cgagccagaa cacgatcgcg 180 gcgctggcgg agacgaaccg catcatgatc gtcggctgcg acccgaaggc ggacagcacg 240 cgcctgatgc tgcacacgaa ggcgcagacg acgatcctgc acctggcggc ggagcgcggc 300 acggtcgagg acatcgagct ggaggaggtc ctgctggagg gctaccaggg cgtcaagtgc 360 gtcgagagcg gcggcccgga gccgggcgtc ggctgcgcgg gccgcggcat catcacggcg 420 atcaacttcc tggaggagga gggcgcgtac gaggacctgg acttcgtcag ctacgacgtc 480 ctgggcgacg tcgtctgcgg cggcttcgcg atgccgatcc gcgagggcaa ggcgcaggag 540 atctacatcg tcacgagcgg cgagatgatg gcgatgtacg cggcgaacaa catcgcgcgc 600 ggcatcctga agtacgcgca cacgggcggc gtccgcctgg gcggcctgat ctgcaacagc 660 cgcaacgtca actgcgaggc ggagctgatc gaggagctgg cgcgccgcct gggcacgcag 720 atgatccact tcgtcccgcg cagcaagcag gtccaggagg cggagctgcg ccgcatgacg 780 gtcatcgagt acagcccgga ccacccgcag gcgcaggagt accgcgagct gagccgcaag 840 atcgagaaca acacgaacct ggtcatcccg acgccgatca cgatggagga gctggaggag 900 ctgctggtcg acttcggcat cctgggcggc gaggacgagt acgagaaggc gctgcaggcg 960 gacaaggcgg cgacgaaggc gtag 984 <210> 30 <211> 1551 <212> DNA <213> Artificial Sequence <220> <223> Derived from Cyanothece ATCC51142 (NIF -D, #42) <400> 30 atggttgcac ctttcactgg tctcaaatct tcagccactt tccctgttac aaagaagcaa 60 aaccttgaca tcacttccat tgctagcaat ggtggaagag ttagctggat ggctactgta 120 gaagataata aaaaattaat tgctgatgta ttatctactt atcctgaaaa agctgctaaa 180 aaaagagcta aacatttagg agtatatgaa gaaggagaag ctgattgtgg agtaaaatct 240 aataaacaat ctttacctgg agtaatgact gctagaggat gtgcttatgc tggatctaaa 300 ggagtagtat ggggacctat taaagatatg gtacatattt ctcatggacc tgtaggatgt 360 ggatattatt cttggtctgg aagaagaaat tattatattg gaactactgg agtagattct 420 tttggaacta tgcaatttac ttctgatttt caagaaagag atattgtatt tggaggagat 480 aaaaaattag ctaaaattat tgatgaaatt gaagaattat ttcctttaaa tggaggagta 540 tctgtacaat ctgaatgtcc tgtaggatta attggagatg atattgaatc tgtagctaga 600 actaaatcta aagaaactgg aaaatctgta gtacctgtaa gatgtgaagg atttagagga 660 gtatctcaat ctttaggaca tcatattgct aatgatatga ttagagattg ggtatttcct 720 actgctgata aagaaaatgc tgaaaaagga tttgaaggaa ctccttatga tgtagctatt 780 attggagatt ataatattgg aggagatgct tggtcttcta gaattttatt agaagaaatt 840 ggattaagag tagtagctca atggtctgga gatggaactt taactgaaat gaaagctact 900 cctaatgtaa aattaaattt aattcattgt tatagatcta tgaattatat ttctagacat 960 atggaagaaa aatatggaat tccttggtta gaatataatt tttttggacc ttctaaaatt 1020 gctgcttctt taagagaaat tgcttctaga tttgatgaaa aaattcaagc taaagctgaa 1080 gaagtaattg aaaaatatag aaaacaatct gaagaaatta ttgctaaata tagacctaga 1140 ttagaaggaa aaactgtaat gatgatggta ggaggattaa gacctagaca tgtagtacct 1200 gcttttaaag atttaggaat ggaaattatt ggaactggat atgaatttgc tcatggagat 1260 gattataaaa gaactactgg atatgtagaa gatgctactt taatttatga tgatgtaact 1320 ggatatgaat ttgaagaatt tgtaaaagaa ttaaaacctg atttagtagc tgctggaatt 1380 aaagaaaaat atgtatttca aaaaatggct ttacctttta gacaaatgca ttcttgggat 1440 tattctggac cttatcatgg atatgatgga tttgctattt ttgctagaga tatggattta 1500 gctttaaatt ctcctacttg gggattaatt ggaactcctt ggaataaata a 1551 <210> 31 <211> 1536 <212> DNA <213> Artificial Sequence <220> <223> Derived from Cyanothece ATCC51142 (NIF-K, #42) <400> 31 atggctcaaa atgtaaataa tattaaagat catgtagatt tatttcatca acctgaatat 60 caagaattat ttgaaaataa aaaacaattt gaaggaatgc ctactgctga aaaagtacaa 120 gaagtagctg aatggactaa atcttgggaa tatagagaaa aaaattttgc tagagaagct 180 ttaactgtaa atcctgctaa agcttgtcaa cctttaggag ctttattagc tgctattgga 240 tttgaaggaa ctttaccttt tgtacatgga tctcaaggat gtgtagctta ttttagaact 300 catttaacta gacattttaa agaacctgta tctgctgtat cttcttctat gactgaaaat 360 gctgctgtat ttggaggatt aaaaaatatg gtagatggat tacaaaattc ttatgcttta 420 tataaaccta aaatgattgc tgtatgtact acttgtatgg ctgaagtaat tggagatgat 480 ttaggagctt ttttaggaaa tgctagacaa gatggagtaa ttcctgatga tttacctgta 540 ccttttgctc atactccttc ttttgtagga tctcatatta ctggatatga ttctatgatg 600 aaatctattt tatctacttt aactgaagga aaaaaaaaag aaactactaa tggaaaaatt 660 aattttattg ctggatttga aacttatatt ggaaatgtaa gagctattaa aaatattatt 720 tctgcttttg atttagaagg aactttatta tctgatactg aaatgtattt agattctcct 780 aatttaggag aatttaaaat gtatcatgaa ggaacttctt tagaagatgc tgctgattct 840 attaatgctg aagctactgt aactttacaa acttatacta ctcctaaaac tagagaatat 900 attgaaaaaa aatggggaca aaaaacttat acttatagac cttggggagt aaaaggaact 960 gatgaatttt taatgggatt atctgaatta actggaaaac ctattcctaa agaatttgaa 1020 attgctagag gaagagctgt agatgctatg actgatactc aagcttgggt acatggaaaa 1080 agagctgctg tatatggaga tcctgattta gtaatgggat tattacaatt tatgttagaa 1140 atgggaattg aacctgtaca tgtattagta aataattcta ctaaagaatt tgaagaagaa 1200 gctaaagctt tattagctgc ttctccttat ggacaacaag ctactgtatg gggaggaaaa 1260 gatttatggc atatgagatc tttattattt actgaacctg tagatttttt agtaggaaat 1320 tcttatgcta aatatttaca aagagatact aaaactcctt taattagaat tggatatcct 1380 atttttgata gacatcattt acatagatat tctactattg gatatgaagg agctattaat 1440 ttattaaatt ggattgctaa tggattaatg gatgaattag atagaaaaac tgatactcct 1500 tctgtaactg atatttcttt tgatttagta agatag 1536 <210> 32 <211> 891 <212> DNA <213> Artificial Sequence <220> <223> Derived from Trichodesmum sp. IMS101 (NIF-H, #101) <400> 32 atgagacaaa ttgcttttta tggaaaagga ggaattggaa aatctactac ttctcaaaat 60 actttagctg ctatggctaa tagacatgga caaagaatta tgattgtagg atgtgatcct 120 aaagctgatt ctactagatt aattttaaat gctaaagctc aaactactgt attacatgta 180 gctgctgaaa gaggagctgt agaagatgta gaattagatg aagtattaaa acctggattt 240 ggaggaatta aatgtgtaga atctggagga cctgaacctg gagtaggatg tgctggaaga 300 ggaattatta ctgctattaa ttttttagaa gaagaaggag cttatactga tttagatttt 360 gtatcttatg atgtattagg agatgtagta tgtggaggat ttgctatgcc tattagagaa 420 aataaagctc aagaaattta tattgtatgt tctggagaaa tgatggctat gtatgctgct 480 aataatattg ctagaggagt attaaaatat gctcatgctg gaggagtaag attaggagga 540 ttaatttgta attctagaaa agtagataga gaaactgaat taattgaaaa tttagctgct 600 agattaaata ctcaaatgat tcattttgta cctagagata atgtagtaca aagagctgaa 660 attagaagaa tgactgtaga acaatatgct cctgaagata atcaagctca agaatatgat 720 caattagctc aaaaaattat taataatgaa aaattaacta ttcctactcc tttagaaatg 780 gatgaattag aagaattatt aattgaattt ggattattag gagatgaaga agatagacaa 840 aaacaaattg ctgctcaaga tgctgctatt aaagctactg ctgctaaata a 891 <210> 33 <211> 1458 <212> DNA <213> Artificial Sequence <220> <223> Derived from Trichodesmum sp. IMS101 (NIF-D #101) <400> 33 atggcttctg aaaaaattga acaaaataaa caattaattc aagaagtatt agatgcttat 60 cctgctaaag ctgctaaaag aagaaaaaaa catttaaatg taattgaaga aaaaggagct 120 gattgtggag taaaatctaa tgtaaaatct gtacctggag taatgactac tagaggatgt 180 gcttttgctg gagctaaagg agtagtatgg ggacctgtaa aagatatggt acatatttct 240 catggacctg taggatgtgg atattattct tgggctggaa gaagaaatta ttataatgga 300 gtaactggag tagatacttt tggaactatg caatttactt ctgattttca agaaagagat 360 attgtatttg gaggagataa aaaattagct aaaattatga atgaaattga agaattattt 420 cctttaaatg ctggaattac tattgaatct gaatgtcctg taggattaat tggagatgat 480 attgaagctg tagctaaaaa agcttctaaa gaattaaata aacctgtagt acctgtaaga 540 tgtgaaggat ttagaggagt atctcaatct ttaggacatc atattgctaa tgatactgta 600 agagattggg tatatgaacc ttctgctaaa gtaactaatg aagaaattgg atttgaaaaa 660 actccttatg atgtatcttt aattgctgat tataatattg gaggagatgg atggtcttct 720 agattattat tagatgaaat tggattaaga gtagtatctc aagctactgg agatggaact 780 tataatgaag tatttatggc tcctagagta aatttaaatt taattcattg ttatagatct 840 atgaattata tttgtagata tatggaagaa gaatatggaa ttccttgggt agaatttaat 900 ttttttggac cttctcaaat tgctaaatct ttaagaaaaa ttgcttcttt ttttgatgat 960 aaaattaaag aaaatactga aaaagtaatt gctagatatc aagaacaagc tgatgctgta 1020 attgctaaat atagacctag attagaagga aaaaaagtaa tgatgatggt aggaggatta 1080 agacctagac atattattcc tgcttttgat gatttaggaa tggaagtaat tggaactgga 1140 tatgaatttg gacatggaga tgattataaa agaactgctg attatgctca agaaggaact 1200 ttaatttatg atgatgtatc tggatatgaa tttgaagaat ttgctaaaaa attaaaacct 1260 gatttaattg cttctggaat taaagaaaaa tatgtatttc aaaaaatggg aatgcctttt 1320 agacaaatgc attcttggga ttattctgga ccttatcatg gatatgatgg atttgctatt 1380 tttgctagag atatggattt agctttaaat tctcctactt ggaatttaat taaagctcct 1440 tggaaacaag ctaaatag 1458 <210> 34 <211> 1539 <212> DNA <213> Artificial Sequence <220> <223> Derived from Trichodesmum sp. IMS101 (NIF-K, #101) <400> 34 atgtctcaaa atgtagataa aattaaagat cattttcaat tatttcaaga acctgaatat 60 caagaaatgt ttgctagaaa aagagaattt gaaggaggag cttctaaaga agaaattgaa 120 agagtaagag aatggactaa atcttgggaa tatagagaaa aaaattttgc tagagaagct 180 ttaactatta atcctgctaa agcttgtcaa cctttaggag ctatttttgc tgctgctgga 240 tttgaaggaa ctttaccttt tgtacatgga tctcaaggat gtgtagctta ttttagatct 300 catttaacta gaaattataa agaacctttt caagctgtat cttcttctat gactgaagat 360 gctgctgtat ttggaggatt aaaaaatatg attgatggat tagctaattc ttatgcttta 420 tataaaccta aaatgattgc tttatgtact acttgtatgg ctgaagtaat tggagatgat 480 ttaggatctt ttattactaa ttctaaaaat gaaggagctg tacctcaaga ttttcctgta 540 ccttttgctc atactccttc ttttgtagga tctcatatta ctggatatga taatatgtta 600 aaaggaattt taattgcttt aactgatgga aaaaaaactg aaactgataa tggaaaaatt 660 aattttattc ctggatttga tccttatatt ggaaatatta gagatttaaa aaatatttta 720 tctttaatgg atgtaccttc tactgtatta gctgataatg ctgaatcttt tgattctcct 780 aatttaggag aatttaaaat gtataatgga ggaactactt tagaagaagc tggagattct 840 attaatgcta aagctactat ttcttttcaa aaatattcta ctcctaaaac tttagaatat 900 ttaaaacaag aaggaggaca aaaaactgct acttatagac ctattggagt aagaggaact 960 gatgaatttt taatggcttt atctgaatta actggaaaag ctattcctga agaattagaa 1020 attgaaagag gaagagtagt agatgctatt actgattctc aagcttggtt acatggaaaa 1080 agaattgcta tttatggaga tcctgatcat gtattaggat tattaaattt tactttagaa 1140 ttaggaatgc aacctgtaca tgtagtagta aataatggaa atgtagctgg atttgaagaa 1200 gaagctaaag aattattagc taatgatcct aatggaaaag aagctactgt atggattgga 1260 aaagatttat ggcatttaag atctttatta gatactgaac ctgtagattt attaattgga 1320 aattcttatg gaaaattttt acaaagagat actggaactc ctttagtaag aattggatat 1380 cctatttttg atagacatca tcaacataga tattctattt taggatataa aggagctttt 1440 aatttaatta attggattgt aaatactatt ttagatgaat tagatagagg atctatggat 1500 ttaggagtaa atgatacttc ttttgattta gtaagataa 1539 <210> 35 <211> 894 <212> DNA <213> Artificial Sequence <220> <223> Derived from Chlorogloeopsos fritschii (NIF-H #912) <400> 35 atgtctatcg ataagaaaat ccgtcaaatc gcattttacg gaaaaggcgg tatcggaaaa 60 tccactacct cacaaaacac tcttgctgca atggctgaaa tgggtcaaag aatccttatc 120 gttggatgcg atcctaaagc agacagtacc cgccttatgc ttcactctaa agctcaaacc 180 accgtacttc atcttgcagc tgaacgtggt gctgttgaag atcttgaatt agaggaagta 240 atgttaactg gttttagagg agttaaatgc gtagagtctg gtggtccaga accaggtgtt 300 ggatgtgcag gtcgaggaat catcactgct atcaatttct tagaggaaaa cggtgcatat 360 caagatgtag attttgtttc atacgatgtt ttaggtgatg tagtttgtgg tggattcgct 420 atgccgatcc gtgagaacaa agcacaagaa atctatatcg taaccagtgg tgagatgatg 480 gctatgtacg cagctaataa catcgctaga ggtatcttaa agtatgcaca tactggaggg 540 gttcgattag gcgggttaat ctgtaattca cgtaacgtag atcgtgaaat cgagttgatc 600 gaaactttgg ctaaaagatt gaatactcaa atgatccatt acgttcctcg cgataacatc 660 gtacaacatg ccgagttgcg tcgcatgaca gtaaatgaat atgcccctga ttccaatcag 720 tctaatgagt atcgtatctt ggcgaataaa atcatcaata atgaaaattt gaaagtacct 780 actcctatcg agatggaaga actagaagaa ctactgatcg aatttggtat cctggaatct 840 gaagaaaatg ctgctaaaat gatcgggact cctgctcagt cttccactaa ataa 894 <210> 36 <211> 1494 <212> DNA <213> Artificial Sequence <220> <223> Derived from Chlorogloeopsos fritschii (NIF-D, #912) <400> 36 atgactcctc cagaaaatca aaacctagta gatgagaata aagagttaat caaagaagtg 60 cttcaagctt accctgaaaa ggcacgaaaa aaaagagaga agcatttaaa tgttcacgaa 120 gaaagtaaat ccgattgtgg tgtaaaatct aacataaagt ccattccagg cgttatgact 180 gctcgtgggt gcgcatatgc tggtagcaaa ggggtagttt ggggtcctat caaagatatg 240 attcatatat cccacgggcc tgtaggttgt gggtactggt cttggtccgg aagacgtaat 300 tattacgttg gtatcaccgg aattaactct ttcggtacta tgcattttac ttccgatttc 360 caggaaagag acatcgtatt tggaggtgat aagaaattga ccaaaataat tgatgagtta 420 gaaatccttt ttccattaaa tcgtggagtg tctattcaat cagaatgccc tatcggtttg 480 attggaggtg atatcgaagc agttgctaaa aaagcatcta agcagtatgg aaaaccagta 540 gttcctttaa gatgtgaggg tctgcgtgga gtatcacaat ctttaggtca tcacattgct 600 aacgacgcaa tcagagattg gattttccca gaatttgata aggctaaaaa aaataatact 660 atcgactttg aaccttcacc gtacgatgtt gcacttattg gagatcataa catcggtgga 720 gatgcttggg catctcgtat gttattggag gaaatgggtc tgagagtagt tgctcaatgg 780 tcaggagatg gtactttaaa tgaacttatt caaggacctg cagctaaatt ggtactgatt 840 cactgctatc gttctatgaa ttacatctgt cgctcattgg aggaacaata tggtatgccg 900 tggatggaat tcaacttttt tggacctact aaaattgcag cttctcttcg tgagatcgca 960 gctaaattcg acagtaaaat tcaagaaaat gcagaaaaag ttatcgctaa atacaccccg 1020 gtaatgaatg cagtgcttga gaaatatcgc cctcgtttgg aaggtaacac tgttatgctt 1080 tacgtagggg gtcttcgccc tcgtcatgtt gtacctgctt tcgaagattt ggggattaaa 1140 gttgtaggta ccgggtatga atttgcacat aatgatgatt acaaacgcac tacacactat 1200 atcgataacg ctactattat ctacgatgat gttacagcct atgagttcga agaatttgta 1260 aaagctaaaa aacctgatct tattgcctct ggtatcaaag aaaaatacgc ttttcagaaa 1320 atgggtctac cttttcgaca aatgcatagt tgggattatt ctggtcctta tcatggttat 1380 gatcgttttg ccatctttgc tcgagatatg gatcttgccc taaatagtcc tacttggagt 1440 ctaatcggtg cgccttggaa aaaagcggct actaaagcga aagctgctgc ttaa 1494 <210> 37 <211> 1539 <212> DNA <213> Artificial Sequence <220> <223> Derived from Chlorogloeopsos fritschii (NIF-K, #912) <400> 37 atgcctcaaa atccagagaa aattgtagat catgtacaat tatttcagca acctgaatat 60 caagaacttt tcaaaaataa acacgaacaa tttgagggag ctcattcccc agaagaagta 120 cagagagtat ccgaatggac taaaggttgg gagtacagag aaaagaactt tgcacgtcaa 180 gctttaacag taaatcctgc aaaaggatgc caaccagtag gtgctatctt cgcagctgta 240 ggatttgaag gtactctacc tttcgtacaa ggatcccagg gttgtgtagc atattttaga 300 acacatttat cccgtcacta caaagaacca ttctctgctg tatcctcatc tatgactgag 360 gatgcagctg tatttggagg tcttaacaat atgattgaag gattacaagt atcatatcaa 420 ctatacaaac ctaaaatgat cgcagtatgt acaacttgta tggctgaagt aattggtgat 480 gatttaggag cattcatcac aaatgctaaa aacgcaggtt ctattccaca agattttcct 540 gtaccattcg ctcatactcc ttcatttgta ggatctcata caaccggtta tgataatatg 600 atgaagggaa tcctttcaaa tttaactgag ggtaaaaaaa aagataagac caacggaaaa 660 attaatttca tcccaggttt tgatacttac gtaggaaata acagagaact gaaacgtatg 720 ttaggtctta tgggaattga ttataccatc ttagcagata attctgatta cctggatgct 780 cctaatactg gtgaatatga tatgtacccg ggcggtacca aattagagga tgcagctgat 840 tcatgcaacg cagaagctac tgtagccctt cagagatata ccactaccaa gactcgagaa 900 tacattgaga ccaaatggaa acaacaaact aaagtattac gtcctttcgg ggtaaaaggt 960 accgacgaat ttcttatgac tttatctgaa atgaccggga aaccgatccc tcaagaactt 1020 gaggatgaac gaggtcgttt agtagacgct atgactgata gttatgcctg gattcacggg 1080 aaaaaattcg ctatctacgg tgatccggac cttatttatt ctgtaaccag ttttttactt 1140 gaattagggg ccgaacctgt acatatcctt tgtaataatg gtgatgaaga gttcaaaaaa 1200 gagatggaag ctattttatc tgcgagtccg tttgggaaac aagctactgt atggatccag 1260 aaagatttgt ggcatcttcg atctttgctt tttactgaac ctgtagattt ttttgtaggt 1320 aacagttacg ggaaatattt gtggcgcgac accaaaattc ctatggtacg tatcggttat 1380 cctctttttg atcgccacca tttgcatcgt tatcctactt tggggtatca aggtggtttg 1440 aatttgctta attgggtagt aaacactatt ttggatgaac ttgatcgcag tagcaatata 1500 gcgggtaaaa ctgatatatc ttttgatttg atccgttaa 1539 <210> 38 <211> 1206 <212> DNA <213> Artificial Sequence <220> <223> Derived from Anabaena (NIF-H, #AB) <400> 38 atggcttcct ctgtcatttc ttcagcagct gttgccacac gcagcaatgt tacacaagct 60 agcatggttg cacctttcac tggtctcaaa tcttcagcca ctttccctgt tacaaagaag 120 caaaaccttg acatcacttc cattgctagc aatggtggaa gagttagctg cgagctcaag 180 cttcgaattc tgcagtcgac ggtaccgggc cccccctcga ggtcgacaat gactgacgaa 240 aacattagac agatagcttt ctacggtaaa ggcggtatcg gtaaatctac cacctcccaa 300 aacacccttg cagctatggc agaaatgggt caacgcatca tgatcgtagg ttgcgaccct 360 aaagctgact ccacccgttt gatgctccac gctaaagcgc aaaccactgt acttcacttg 420 gctgctgaac gcggtgcagt agaagactta gaactacacg aagtaatgtt gaccggtttc 480 cgtggcgttc gttgcgtaga atctggtggt ccagaacccg gtgtaggttg cgccggtcgt 540 ggtatcatca ccgccattaa cttcttagaa gaaaacggtg cttaccaaga cctagacttc 600 gtatcctacg acgtattggg tgacgttgta tgtggtggtt tcgctatgcc tatccgtgaa 660 ggtaaagcac aagaaatcta catcgttacc tctggtgaaa tgatggcgat gtatgctgct 720 aacaacatcg ctcgcggtat tttgaaatat gctcactccg gtggtgtacg cttgggtggt 780 ttgatttgta acagccgtaa aactgaccgg gaagccgaat tgatcgaaaa cttggctgaa 840 cgtttgaaca ctcaaatgat tcacttcgta cctcgtgaca acatcgttca acacgctgaa 900 ttgcgtcgga tgactgttaa cgagtacgca ccagacagca accaaggtca agagtaccgt 960 gcattagcca agaaaatcat caacaacgac aagctcacca ttcctacacc aattgaaatg 1020 gatgaactcg aagccctgtt gatcgaatac ggtattcttg atgatgattc taagcacgca 1080 gaaatcatcg gtaagcccgc agaagctacc aaattggatc cacctgatct agagtccgca 1140 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt 1200 gagtag 1206 <210> 39 <211> 1494 <212> DNA <213> Artificial Sequence <220> <223> Derived from Anabaena (NIF-D, #AB) <400> 39 atgacacctc ctgaaaacaa gaatcttgta gatgaaaata aagaacttat taaagaagtt 60 ctgaaagctt atcccgaaaa atctcgcaaa aaacgcgaaa agcacctcaa cgtccacgaa 120 gaaaacaagt ctgattgcgg cgtaaagtct aacatcaaat ccgttcctgg tgtaatgacc 180 gctcgtggtt gtgcttatgc aggttctaag ggtgtggttt ggggtcctat taaggacatg 240 attcacatca gccacgggcc tgtaggttgc ggttactggt cttggtctgg tcgtcgtaac 300 tactacgttg gtgtaactgg tatcaactct ttcggtacca tgcactttac atcagacttc 360 caagaacgtg acatcgtgtt cggtggtgac aaaaaactcg ttaaactcat tgaagaactt 420 gacgttctgt tccctctaaa ccgtggtgtt tccattcaat ctgaatgtcc catcggtcta 480 attggggatg acatcgaagc tgtagctaag aaaacttcta agcaaattgg taagcctgtt 540 gtacccttac gttgcgaagg tttccgtggt gtatctcagt ctttaggaca ccacatcgct 600 aacgacgcta tccgtgactg gattttccca gaatacgaca agctgaagaa agaaaacaga 660 ctcgacttcg agccaagccc ctatgatgta gccctaatcg gtgactacaa catcggtggt 720 gacgcttggg ctagccgtat gttgttggaa gaaatgggct tacgtgttgt agctcagtgg 780 tctggtgatg gtactcttaa cgagttgatc caaggccctg ctgctaagtt agtcctcatc 840 cactgctacc gttctatgaa ctacatctgc cgtagtttgg aagaacaata tggtatgcct 900 tggatggagt tcaacttctt cggccccacc aagattgctg cttccttacg tgaaatcgca 960 gctaagtttg attccaagat tcaagaaaac gctgagaagg taattgctaa gtacacacca 1020 gtaatgaatg ctgtacttga taagtaccgt cctcgtttgg aaggtaacac cgtaatgttg 1080 tacgtaggtg gtctacgtcc tcgtcacgtt gttcctgctt ttgaagattt gggtatcaaa 1140 gttatcggta caggttacga gttcgcccac aacgacgact acaaacgtac cacccactac 1200 atcgataacg ccaccatcat ttatgatgac gttaccgcct acgaatttga agagttcgta 1260 aaagctaaga agcctgattt aatcgcttct ggtattaaag agaagtatgt cttccaaaag 1320 atggctcttc ccttccgtca aatgcactct tgggattact ccggccctta tcacggttac 1380 gacggatttg ctatcttcgc ccgtgacatg gatttagcgc tcaacagccc cacttgggac 1440 ttgattggcg ctccttggaa gaaagctgct acaaaagcta aggctgctgc ctaa 1494 <210> 40 <211> 1539 <212> DNA <213> Artificial Sequence <220> <223> Derived from Anabaena (NIF-K, #AB) <400> 40 atgcctcaga atccagaaag aattgtagac cacgttgatc tattcaaaca gccagaatac 60 accgagctat ttgaaaacaa gagaaagaac tttgaaggcg ctcatcctcc tgaagaagtt 120 gaaagagtgt ctgaatggac aaaatcttgg gactaccgcg aaaagaactt cgctcgtgaa 180 gctttaaccg ttaaccctgc taaaggttgc caacctgtag gcgcgatgtt cgctgctttg 240 ggttttgaag gtactctacc tttcgtacaa ggttctcaag gttgcgttgc ttacttccgt 300 acacacctca gccgtcacta caaagagcct tgctccgcag tatcttcttc catgacagaa 360 gacgcagcag tattcggtgg cttgaacaac atggttgaag gtatgcaggt tgcttaccaa 420 ctgtacaagc ctaagatgat tgctgtttgc accacctgta tggctgaggt tatcggtgat 480 gacttaggtg cgttcatcac caactccaag aatgctggtt ctattcctca agatttccct 540 gtaccctttg ctcacacccc cagtttcgtt ggttctcacg taactggtta cgacaacatg 600 atgaagggta ttctgtctaa cttgacagaa ggtaagaaga aagctaccag caacggtaaa 660 atcaacatca tccctggttt tgatacctat gtaggtaaca accgcgaagt taagcggatg 720 ttgggtgtaa tgggtgttga ttacacaatc ctgtctgaca gcagcgacta ctttgattca 780 ccaaacactg gtgagtacga aatgtaccca ggtggtacca agttggaaga tgcggctgat 840 tctatcaacg ctaaagctac tgttgctctc caagcttaca ccacacccaa gacccgtgag 900 tatatcaaaa cccagtggaa gcaagaaaca caagtattgc gcccctttgg tgttaagggt 960 actgacgagt tcttaacagc tatctctgaa ttgaccggta aggctattcc tgaagagttg 1020 gaaatcgaac gcggtcgttt agttgatgct atcaccgact cttacgcttg gattcatggt 1080 aagaagttcg ctatttacgg cgatccagat ttgatcatct ccatcaccag cttcttgtta 1140 gagatgggtg ctgaaccagt acacatcctc tgcaacaacg gtgatgacac cttcaaaaaa 1200 gaaatggaag ctatcctcgc tgctagccca tttggtaaag aagctaaagt ctggattcaa 1260 aaagacttgt ggcacttccg ttccttgttg ttcaccgagc ctgtagactt cttcatcggt 1320 aactcctacg gtaagtacct gtggcgcgat accaagatcc caatggtgcg gattggttat 1380 cctctcttcg atcgccacca cttacaccgt tattctactc tcggctacca aggtggtcta 1440 aatatcctca actgggttgt taacaccctg ttggatgaaa tggatcgcag caccaacatc 1500 actggtaaga ccgatatctc ctttgacttg atccgctaa 1539 <210> 41 <211> 255 <212> DNA <213> Solanum lycopersicum <400> 41 atggctagcg ctaccggact cagatctatg gcttcctctg tcatttcttc agcagctgtt 60 gccacacgca gcaatgttac acaagctagc atggttgcac ctttcactgg tctcaaatct 120 tcagccactt tccctgttac aaagaagcaa aaccttgaca tcacttccat tgctagcaat 180 ggtggaagag ttagctggag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc 240 gggatccacc tgatc 255 <210> 42 <211> 1044 <212> DNA <213> Arabidopsis thaliana <400> 42 gagtctctgt cagaagtcag aactatctct ctctcttgac ttgtctaggt gactcttata 60 gagctgtgaa tttaaatgga gttggattgg tgtgatggtg aagataaatt cagtgtcaat 120 tttggatggc tggtgcagtt ttttttttcc ctcttaacga aaacaaaatt gaatttgtta 180 accaaataaa gtaatggaat cttcataagt caaactcatt tttgatgtag ttaaccaaat 240 aatgtcttca ctttctttct caatcaatta ttaacttctt gtgagaaata aactttccta 300 ataatttgtt cacgtataaa tgttttttta ggttgaaaat ttgaagtaca attttctctt 360 attagttttt gttttcaaga tatatctttg aattaatatt atataaaaac gcagcatgta 420 gtagtataac attttgatct tgaaaataaa aaaatatcaa tttcaaagtc ttgaaaataa 480 cctttttttt ttttggctat gagtcttgtg aagttaactt ggtgaactct cgactcacga 540 tgcatatctt aattgagcaa catgattcat aattcatctt ttcatgtctc ttgccaactt 600 ccaatagagt gcattgacat tattttttta atacattata catatatcat gcatgtttgt 660 ttctcatcca tggagaatca tttttgaaat ttttggattt ttgaaattgc ttaaaaagaa 720 agaaaaaaac tcaattttta ttgtcataca atgttttcca aaatatgtgt acatgttctt 780 gcatatgagt taattagtga gggtttcctc aaaaagattg ttgtattttt ggaaaacaaa 840 gtttattttt ggatattggt tctgcatttt acatctaatt tgttttttaa atcataattt 900 tagaaaatct attactaaat taagaaatct atgaatggtt tacttcattc cgtaaaacac 960 tataaattgt cctaaactca tattcgctct caagtcaaaa ctcttctatt ttcccctgtc 1020 ttcatatcca acagagatct cttc 1044 <210> 43 <211> 1386 <212> DNA <213> Solanum lycopersicum <400> 43 atatcttttt gtcatgatat aatatgtttc ttttagcaaa aatgaaatag ttccgtcatt 60 tatcggatca tatttagata gattgttgta gatgacactt ttatgaaatt acaataatca 120 taaatttatc acgggcacaa ttagcgacaa tgaagaccaa ccaaagttag ggggcaaaaa 180 ccgaacaggc caaccaaagt tatgggtgtg caaaaaatcg aatcgatcga taaatcgaat 240 cgaaaaaatg ttattgggtt atttatgttt tttcgtgggt ttataaaaaa aattattgaa 300 ttattggttc ggtttcaatt tttattattg ggttattggg taaaccgata atccaataag 360 acggtaataa tttttatttt acccttcata attatttatt attagcaagt taatatataa 420 ttagacacta taattatatc aaattattag tactctacca acttcagagt tggctgattt 480 actagttttt attgtttatt caaaacctaa gaattaaagt aatgcgttca caattgcagt 540 tatttgattt taattttagt tttagtctta ttggactgtt ttattttagt tttaggttat 600 aatggcaggt tatagcattt gcatcttagt aaaggtcagt aatttgatta acacaaaaaa 660 ttccatacta tattttggtg gtaatatgta attatagcat tcgtgctatt ttctcgtatt 720 gatacaattt ctcattatct ttcttgtttt actatatcaa aacatttaga gaagtgagaa 780 gacatataat attttacgga cattttctta ttgggtaaac tgaaaatcga atcgataatg 840 ataaaaaact gataaattga aatctgataa aaaatatctt gttgatttgt tattgaatta 900 acatatttaa aagctgaaaa ccgataaatc gaacgataat atataaaatc gaaccgaacc 960 gatcgatgca cacccttgac caaatctgaa gcacatattt atcgatctaa attttattaa 1020 agagattaat atcgaataat catatacata tttcatatgt ataacaaatt tcaaatacac 1080 gtatctaata tatcgagtga tgcgacaaat acatgtatcg gacgcaccaa ttgatataga 1140 aaacgtaata ttgaaaacta atgtaaagaa aagtaacttg atcctaaact aatcaagata 1200 agcccaataa atatacattg tcatctccaa aggcccaaaa atggcacaag atggcaggcc 1260 caataacgaa gaaaagggct tgtaaaaccc taataaagtg gcactggcag agcttacact 1320 ttcattccat caacaaaaga aaccctagaa gccgcagtgc cactgatttc tctcctccag 1380 acgaag 1386 <210> 44 <211> 333 <212> DNA <213> Cauliflower mosaic virus <400> 44 aaacatgtgg agcacgacac acttgtctac tccaaaaata tcaaagatac agtctcagaa 60 gaccaaaggg caattgagac ttttcaacaa agggtaatat ccggaaacct cctcggattc 120 cattgcccag ctatctgtca ctttattgtg aagatagtgg aaaaggaagg tggctcctac 180 aaatgccatc attgcgataa aggaaaggcc atcgttgaag atgcctctgc cgacagtggt 240 cccaaagatg gacccccacc cacgaggagc atcgtggaaa aagaagacgt tccaaccacg 300 tcttcaaagc aagtggattg atgtgataac atg 333 <210> 45 <211> 1374 <212> DNA <213> Solanum lycopersicum <400> 45 aatattaggt ggtgatcacg ataggatact tgaaaaaaaa tattgcaagt agacaaacct 60 tgtagtaatt gagacaaaat atcagtagct gtcaacacca caaatatcat aaatacctca 120 ctggctgtgg ggtttgtgct cgtgataaaa cttatatatt agtaataaaa aagaaacata 180 gccacgtgaa tgaaataatg gaggtgaaaa cagtaatgaa atgacaataa ttgagttctt 240 gatccaaatg attgtttaag tttccaaatt gtttctagta actcaacgca aaatattctt 300 caacactttt tgacaaaatt tgattggatc taaactagaa ctagtttgta ggctttgtgt 360 gttaagtgat tacaataagg ttaataaata gagtaattca aatcaaagca aaaacagaaa 420 aagatcaagt tgagtagaga acttgatcac gatttaaaca ttttactata tatatgagga 480 gctacattaa ggataataag acaatttcat gctcaacatg gttataattc aaactgttca 540 aagcaccatc cagtaatagg tcctacagtg tgtgatttaa ttttaattaa agttttatac 600 atcaaatata gacgaaaaaa ataaaaaata aatcttattt ttgataatat atataaattt 660 gaatgcatta tgtttaataa ctatctattt catctcgtgt tgtttgtcct tttaaagatt 720 cttttttata tttaatgact aagctaaatg tatagtaaat gcgttgaatt tgactgaact 780 caaacaaaaa aacataggaa tattacattt caacgcaata actcaaatgg tcaatgcatt 840 cacacgctaa atcaacattt ttatatatac gcattaccac ttatcaatct attaagattt 900 attcatctcc ttaaaaaaag acacatttaa ttcctttaat tataaaggta tttatcaaaa 960 aacacccaaa acattacata tatctttctc ataacatctt atatacattt actaaaaata 1020 ataactagta actaatttct tttctaaaaa tacacatttt ttttgaaaga aaagaaaata 1080 cttatttgag atgataaatt aatttattgg gcgttaagaa gagaacaaaa agaaaataat 1140 ggatgccaaa aatatcggtg gtgaaaatcg ggggacccat attcttctca gcctataatg 1200 tagggtccat cagttattgc gtgtttatgt attcccgcct ctctcctcat taccactaga 1260 aatatcactc tctttcccat ttctcccttc atcttcatct tcatcttcat ctcttcttct 1320 tcttattctc tcattcctct catcaatttt tttcatcaaa aaaactaaga gaaa 1374 <210> 46 <211> 16890 <212> DNA <213> Artificial Sequence <220> <223> Plasmid DNA host by E.Coli Xl1B and or Agrobacteriun EHA105-transformed into plant cell <400> 46 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga 60 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc 120 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg 180 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc 240 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg 300 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga 360 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt 420 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat 480 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag 540 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc 600 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa 660 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc 720 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc 780 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg 840 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc 900 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg 960 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc 1020 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg 1080 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg 1140 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg 1200 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca 1260 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc 1320 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg 1380 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc 1440 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag 1500 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca 1560 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc 1620 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg 1680 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta 1740 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga 1800 caatctgatc caagctcaag ctaagcttca gagccaccac cctcagagcc gccaccagaa 1860 ccaccaccag agccgccgcc agcattgaca ggaggcccga tctagtaaca tagatgacac 1920 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat 1980 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat 2040 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc 2100 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggatcat 2160 ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtccat 2220 ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccgg gcccgatcat 2280 attgtcgctc aggatcgtgg cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc 2340 accttcgacc gcctgttccg cagagatccc gtgggcgaag aactccagca tgagatcccc 2400 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata 2460 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat 2520 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc 2580 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca 2640 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc 2700 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag 2760 caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg 2820 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca 2880 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat 2940 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact 3000 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc 3060 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc 3120 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg 3180 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca 3240 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc 3300 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccaga tccggtgcag 3360 attatttgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa 3420 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt 3480 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg 3540 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc 3600 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcagattg 3660 tcgtttcccg ccttcaggta ccgcgatcgc tcgcgacctg caggcataaa gccgtcagtg 3720 tccgcataaa gaaccaaccc ataataccca taatagctgt ttgccaaccg gtcaacatgt 3780 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag 3840 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc 3900 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca 3960 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga 4020 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa 4080 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat 4140 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc 4200 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga 4260 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga 4320 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 4380 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 4440 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 4500 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct 4560 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa 4620 gcaattttct gaaaattttc accatttacg aacgatagcc atggctagcg ctaccggact 4680 cagatctatg gcttcctctg tcatttcttc agcagctgtt gccacacgca gcaatgttac 4740 acaagctagc atggttgcac ctttcactgg tctcaaatct tcagccactt tccctgttac 4800 aaagaagcaa aaccttgaca tcacttccat tgctagcaat ggtggaagag ttagctgcag 4860 agctcaaatg ggccgcaaca gccagggctt cctgacgacg atccgcatca tcgtcacgag 4920 cgcgacggac cagccgagca acttcatcca cagcctgagc aacaagcgcg agagcacgat 4980 gcgccagatc gcgttctacg gcaagggcgg catcggcaag agcacgacga gccagaacac 5040 gatcgcggcg ctggcggaga cgaaccgcat catgatcgtc ggctgcgacc cgaaggcgga 5100 cagcacgcgc ctgatgctgc acacgaaggc gcagacgacg atcctgcacc tggcggcgga 5160 gcgcggcacg gtcgaggaca tcgagctgga ggaggtcctg ctggagggct accagggcgt 5220 caagtgcgtc gagagcggcg gcccggagcc gggcgtcggc tgcgcgggcc gcggcatcat 5280 cacggcgatc aacttcctgg aggaggaggg cgcgtacgag gacctggact tcgtcagcta 5340 cgacgtcctg ggcgacgtcg tctgcggcgg cttcgcgatg ccgatccgcg agggcaaggc 5400 gcaggagatc tacatcgtca cgagcggcga gatgatggcg atgtacgcgg cgaacaacat 5460 cgcgcgcggc atcctgaagt acgcgcacac gggcggcgtc cgcctgggcg gcctgatctg 5520 caacagccgc aacgtcaact gcgaggcgga gctgatcgag gagctggcgc gccgcctggg 5580 cacgcagatg atccacttcg tcccgcgcag caagcaggtc caggaggcgg agctgcgccg 5640 catgacggtc atcgagtaca gcccggacca cccgcaggcg caggagtacc gcgagctgag 5700 ccgcaagatc gagaacaaca cgaacctggt catcccgacg ccgatcacga tggaggagct 5760 ggaggagctg ctggtcgact tcggcatcct gggcggcgag gacgagtacg agaaggcgct 5820 gcaggcggac aaggcggcga cgaaggcgta gtaggatcca cctgatctag agtccgcaaa 5880 aatcaccagt ctctctctac aaatctatct ctctctattt ttctccagaa taatgtgtga 5940 gtagttccca gataagggaa ttagggttct tatagggttt cgctcatgtg ttgagcatat 6000 aagaaaccct tagtatgtat ttgtatttgt aaaatacttc tatcaataaa atttctaatt 6060 cctaaaacca aaatccagtg acgcggccgc acccataata cccataatag ctgtttgcca 6120 tcgctacctt aggaccgtta tagttaaccg gtcaacatgt ggagcacgac acacttgtct 6180 actccaaaaa tatcaaagat acagtctcag aagaccaaag ggcaattgag acttttcaac 6240 aaagggtaat atccggaaac ctcctcggat tccattgccc agctatctgt cactttattg 6300 tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca tcattgcgat aaaggaaagg 6360 ccatcgttga agatgcctct gccgacagtg gtcccaaaga tggaccccca cccacgagga 6420 gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa gcaagtggat tgatgtgata 6480 acatggtgga gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag 6540 accaaagggc aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc 6600 attgcccagc tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca 6660 aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc 6720 ccaaagatgg acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt 6780 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc 6840 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggacgt 6900 cgagagttct caacacaaca tatacaaaac aaacgaatct caagcaatca agcattctac 6960 ttctattgca gcaatttaaa tcatttcttt taaagcaaaa gcaattttct gaaaattttc 7020 accatttacg aacgatagcc atggctagcg ctaccggact cagatctatg gcttcctctg 7080 tcatttcttc agcagctgtt gccacacgca gcaatgttac acaagctagc atggttgcac 7140 ctttcactgg tctcaaatct tcagccactt tccctgttac aaagaagcaa aaccttgaca 7200 tcacttccat tgctagcaat ggtggaagag ttagctgcag agctcaaatg gttgcacctt 7260 tcactggtct caaatcttca gccactttcc ctgttacaaa gaagcaaaac cttgacatca 7320 cttccattgc tagcaatggt ggaagagtta gctggatggc tactgtagaa gataataaaa 7380 aattaattgc tgatgtatta tctacttatc ctgaaaaagc tgctaaaaaa agagctaaac 7440 atttaggagt atatgaagaa ggagaagctg attgtggagt aaaatctaat aaacaatctt 7500 tacctggagt aatgactgct agaggatgtg cttatgctgg atctaaagga gtagtatggg 7560 gacctattaa agatatggta catatttctc atggacctgt aggatgtgga tattattctt 7620 ggtctggaag aagaaattat tatattggaa ctactggagt agattctttt ggaactatgc 7680 aatttacttc tgattttcaa gaaagagata ttgtatttgg aggagataaa aaattagcta 7740 aaattattga tgaaattgaa gaattatttc ctttaaatgg aggagtatct gtacaatctg 7800 aatgtcctgt aggattaatt ggagatgata ttgaatctgt agctagaact aaatctaaag 7860 aaactggaaa atctgtagta cctgtaagat gtgaaggatt tagaggagta tctcaatctt 7920 taggacatca tattgctaat gatatgatta gagattgggt atttcctact gctgataaag 7980 aaaatgctga aaaaggattt gaaggaactc cttatgatgt agctattatt ggagattata 8040 atattggagg agatgcttgg tcttctagaa ttttattaga agaaattgga ttaagagtag 8100 tagctcaatg gtctggagat ggaactttaa ctgaaatgaa agctactcct aatgtaaaat 8160 taaatttaat tcattgttat agatctatga attatatttc tagacatatg gaagaaaaat 8220 atggaattcc ttggttagaa tataattttt ttggaccttc taaaattgct gcttctttaa 8280 gagaaattgc ttctagattt gatgaaaaaa ttcaagctaa agctgaagaa gtaattgaaa 8340 aatatagaaa acaatctgaa gaaattattg ctaaatatag acctagatta gaaggaaaaa 8400 ctgtaatgat gatggtagga ggattaagac ctagacatgt agtacctgct tttaaagatt 8460 taggaatgga aattattgga actggatatg aatttgctca tggagatgat tataaaagaa 8520 ctactggata tgtagaagat gctactttaa tttatgatga tgtaactgga tatgaatttg 8580 aagaatttgt aaaagaatta aaacctgatt tagtagctgc tggaattaaa gaaaaatatg 8640 tatttcaaaa aatggcttta ccttttagac aaatgcattc ttgggattat tctggacctt 8700 atcatggata tgatggattt gctatttttg ctagagatat ggatttagct ttaaattctc 8760 ctacttgggg attaattgga actccttgga ataaataagt aggatccacc tgatctagag 8820 tccgcaaaaa tcaccagtct ctctctacaa atctatctct ctctattttt ctccagaata 8880 atgtgtgagt agttcccaga taagggaatt agggttctta tagggtttcg ctcatgtgtt 8940 gagcatataa gaaaccctta gtatgtattt gtatttgtaa aatacttcta tcaataaaat 9000 ttctaattcc taaaaccaaa atccagtgac gcggccgcat taccctgtta tccctaaccg 9060 gtcaacatgt ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag 9120 aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat 9180 tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct 9240 acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg 9300 gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca 9360 cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact 9420 ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa 9480 gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga 9540 agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca 9600 tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca 9660 tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct 9720 ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat 9780 aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac 9840 aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt 9900 taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagcc atggctagcg 9960 ctaccggact cagatctatg gcttcctctg tcatttcttc agcagctgtt gccacacgca 10020 gcaatgttac acaagctagc atggttgcac ctttcactgg tctcaaatct tcagccactt 10080 tccctgttac aaagaagcaa aaccttgaca tcacttccat tgctagcaat ggtggaagag 10140 ttagctgcag agctcaaatg gctcaaaatg taaataatat taaagatcat gtagatttat 10200 ttcatcaacc tgaatatcaa gaattatttg aaaataaaaa acaatttgaa ggaatgccta 10260 ctgctgaaaa agtacaagaa gtagctgaat ggactaaatc ttgggaatat agagaaaaaa 10320 attttgctag agaagcttta actgtaaatc ctgctaaagc ttgtcaacct ttaggagctt 10380 tattagctgc tattggattt gaaggaactt taccttttgt acatggatct caaggatgtg 10440 tagcttattt tagaactcat ttaactagac attttaaaga acctgtatct gctgtatctt 10500 cttctatgac tgaaaatgct gctgtatttg gaggattaaa aaatatggta gatggattac 10560 aaaattctta tgctttatat aaacctaaaa tgattgctgt atgtactact tgtatggctg 10620 aagtaattgg agatgattta ggagcttttt taggaaatgc tagacaagat ggagtaattc 10680 ctgatgattt acctgtacct tttgctcata ctccttcttt tgtaggatct catattactg 10740 gatatgattc tatgatgaaa tctattttat ctactttaac tgaaggaaaa aaaaaagaaa 10800 ctactaatgg aaaaattaat tttattgctg gatttgaaac ttatattgga aatgtaagag 10860 ctattaaaaa tattatttct gcttttgatt tagaaggaac tttattatct gatactgaaa 10920 tgtatttaga ttctcctaat ttaggagaat ttaaaatgta tcatgaagga acttctttag 10980 aagatgctgc tgattctatt aatgctgaag ctactgtaac tttacaaact tatactactc 11040 ctaaaactag agaatatatt gaaaaaaaat ggggacaaaa aacttatact tatagacctt 11100 ggggagtaaa aggaactgat gaatttttaa tgggattatc tgaattaact ggaaaaccta 11160 ttcctaaaga atttgaaatt gctagaggaa gagctgtaga tgctatgact gatactcaag 11220 cttgggtaca tggaaaaaga gctgctgtat atggagatcc tgatttagta atgggattat 11280 tacaatttat gttagaaatg ggaattgaac ctgtacatgt attagtaaat aattctacta 11340 aagaatttga agaagaagct aaagctttat tagctgcttc tccttatgga caacaagcta 11400 ctgtatgggg aggaaaagat ttatggcata tgagatcttt attatttact gaacctgtag 11460 attttttagt aggaaattct tatgctaaat atttacaaag agatactaaa actcctttaa 11520 ttagaattgg atatcctatt tttgatagac atcatttaca tagatattct actattggat 11580 atgaaggagc tattaattta ttaaattgga ttgctaatgg attaatggat gaattagata 11640 gaaaaactga tactccttct gtaactgata tttcttttga tttagtaaga taggatccac 11700 ctgatctaga gtccgcaaaa atcaccagtc tctctctaca aatctatctc tctctatttt 11760 tctccagaat aatgtgtgag tagttcccag ataagggaat tagggttctt atagggtttc 11820 gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct 11880 atcaataaaa tttctaattc ctaaaaccaa aatccagtga cgcggccgca ttaccctgtt 11940 atccctatta attaagagct cgctacctta agagaggata tcggcgcgcc tctagaattt 12000 aaatggatcc tacgtactcg aggaattcaa ttcggcgtta attcagtaca ttaaaaacgt 12060 ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt acaccacaat atatcctgcc 12120 accagccagc caacagctcc ccgaccggca gctcggcaca aaatcaccac tcgatacagg 12180 cagcccatca gtccgggacg gcgtcagcgg gagagccgtt gtaaggcggc agactttgct 12240 catgttaccg atgctattcg gaagaacggc aactaagctg ccgggtttga aacacggatg 12300 atctcgcgga gggtagcatg ttgattgtaa cgatgacaga gcgttgctgc ctgtgatcaa 12360 ttcgggcacg aacccagtgg acataagcct cgttcggttc gtaagctgta atgcaagtag 12420 cgtaactgcc gtcacgcaac tggtccagaa ccttgaccga acgcagcggt ggtaacggcg 12480 cagtggcggt tttcatggct tcttgttatg acatgttttt ttggggtaca gtctatgcct 12540 cgggcatcca agcagcaagc gcgttacgcc gtgggtcgat gtttgatgtt atggagcagc 12600 aacgatgtta cgcagcaggg cagtcgccct aaaacaaagt taaacatcat gggggaagcg 12660 gtgatcgccg aagtatcgac tcaactatca gaggtagttg gcgtcatcga gcgccatctc 12720 gaaccgacgt tgctggccgt acatttgtac ggctccgcag tggatggcgg cctgaagcca 12780 cacagtgata ttgatttgct ggttacggtg accgtaaggc ttgatgaaac aacgcggcga 12840 gctttgatca acgacctttt ggaaacttcg gcttcccctg gagagagcga gattctccgc 12900 gctgtagaag tcaccattgt tgtgcacgac gacatcattc cgtggcgtta tccagctaag 12960 cgcgaactgc aatttggaga atggcagcgc aatgacattc ttgcaggtat cttcgagcca 13020 gccacgatcg acattgatct ggctatcttg ctgacaaaag caagagaaca tagcgttgcc 13080 ttggtaggtc cagcggcgga ggaactcttt gatccggttc ctgaacagga tctatttgag 13140 gcgctaaatg aaaccttaac gctatggaac tcgccgcccg actgggctgg cgatgagcga 13200 aatgtagtgc ttacgttgtc ccgcatttgg tacagcgcag taaccggcaa aatcgcgccg 13260 aaggatgtcg ctgccgactg ggcaatggag cgcctgccgg cccagtatca gcccgtcata 13320 cttgaagcta gacaggctta tcttggacaa gaagaagatc gcttggcctc gcgcgcagat 13380 cagttggaag aatttgtcca ctacgtgaaa ggcgagatca ccaaggtagt cggcaaataa 13440 tgtctagcta gaaattcgtt caagccgacg ccgcttcgcc ggcgttaact caagcgatta 13500 gatgcactaa gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat 13560 ttttaagcgt gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat 13620 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc 13680 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct 13740 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg 13800 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca 13860 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc 13920 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga 13980 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac 14040 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga 14100 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag 14160 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg 14220 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag 14280 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc 14340 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc 14400 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct 14460 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct 14520 cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt 14580 gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 14640 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 14700 cagaggtttt caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg 14760 gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc 14820 agccattttt gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga 14880 gcgcagcgac cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag 14940 ttatgcacag gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga 15000 aaaatcgcct tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta 15060 cggctttggg ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat 15120 gtacgtgcta tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg 15180 ccctagcatc tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg 15240 gtagcgcatg actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg 15300 caggtcattt gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc 15360 gctgccactg cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc 15420 gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg 15480 gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc 15540 tagctcgatc tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct 15600 aatcaaggct tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg 15660 ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg 15720 ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac 15780 gcggccgggc ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga 15840 aaccgccatc agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga 15900 aacctctacg tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg 15960 cttcgacaga cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc 16020 atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga 16080 cggcgcaccg gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg 16140 ccacgattca ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc 16200 cttcaacttc tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg 16260 tttgcgaccg tcacgccgat tcctcgggct tgggggttcc agtgccattg cagggccggc 16320 agacaaccca gccgcttacg cctggccaac cgcccgttcc tccacacatg gggcattcca 16380 cggcgtcggt gcctggttgt tcttgatttt ccatgccgcc tcctttagcc gctaaaattc 16440 atctactcat ttattcattt gctcatttac tctggtagct gcgcgatgta ttcagatagc 16500 agctcggtaa tggtcttgcc ttggcgtacc gcgtacatct tcagcttggt gtgatcctcc 16560 gccggcaact gaaagttgac ccgcttcatg gctggcgtgt ctgccaggct ggccaacgtt 16620 gcagccttgc tgctgcgtgc gctcggacgg ccggcactta gcgtgtttgt gcttttgctc 16680 attttctctt tacctcatta actcaaatga gttttgattt aatttcagcg gccagcgcct 16740 ggacctcgcg ggcagcgtcg ccctcgggtt ctgattcaag aacggttgtg ccggcggcgg 16800 cagtgcctgg gtagctcacg cgctgcgtga tacgggactc aagaatgggc agctcgtacc 16860 cggccagcgc ctcggcaacc tcaccgccgt 16890 <210> 47 <211> 16728 <212> DNA <213> Artificial Sequence <220> <223> Plasmid DNA host by E.Coli Xl1B and or Agrobacteriun EHA105-transformed into plant cell <400> 47 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga 60 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc 120 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg 180 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc 240 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg 300 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga 360 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt 420 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat 480 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag 540 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc 600 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa 660 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc 720 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc 780 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg 840 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc 900 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg 960 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc 1020 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg 1080 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg 1140 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg 1200 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca 1260 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc 1320 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg 1380 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc 1440 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag 1500 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca 1560 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc 1620 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg 1680 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta 1740 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga 1800 caatctgatc caagctcaag ctaagcttca gagccaccac cctcagagcc gccaccagaa 1860 ccaccaccag agccgccgcc agcattgaca ggaggcccga tctagtaaca tagatgacac 1920 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat 1980 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat 2040 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc 2100 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggatcat 2160 ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtccat 2220 ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccgg gcccgatcat 2280 attgtcgctc aggatcgtgg cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc 2340 accttcgacc gcctgttccg cagagatccc gtgggcgaag aactccagca tgagatcccc 2400 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata 2460 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat 2520 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc 2580 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca 2640 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc 2700 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag 2760 caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg 2820 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca 2880 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat 2940 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact 3000 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc 3060 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc 3120 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg 3180 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca 3240 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc 3300 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccaga tccggtgcag 3360 attatttgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa 3420 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt 3480 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg 3540 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc 3600 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcagattg 3660 tcgtttcccg ccttcaggta ccgcgatcgc tcgcgacctg caggcataaa gccgtcagtg 3720 tccgcataaa gaaccaaccc ataataccca taatagctgt ttgccaaccg gtcaacatgt 3780 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag 3840 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc 3900 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca 3960 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga 4020 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa 4080 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat 4140 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc 4200 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga 4260 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga 4320 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 4380 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 4440 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 4500 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct 4560 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa 4620 gcaattttct gaaaattttc accatttacg aacgatagcc atggctagcg ctaccggact 4680 cagatctatg gcttcctctg tcatttcttc agcagctgtt gccacacgca gcaatgttac 4740 acaagctagc atggttgcac ctttcactgg tctcaaatct tcagccactt tccctgttac 4800 aaagaagcaa aaccttgaca tcacttccat tgctagcaat ggtggaagag ttagctgcag 4860 agctcaaatg agacaaattg ctttttatgg aaaaggagga attggaaaat ctactacttc 4920 tcaaaatact ttagctgcta tggctaatag acatggacaa agaattatga ttgtaggatg 4980 tgatcctaaa gctgattcta ctagattaat tttaaatgct aaagctcaaa ctactgtatt 5040 acatgtagct gctgaaagag gagctgtaga agatgtagaa ttagatgaag tattaaaacc 5100 tggatttgga ggaattaaat gtgtagaatc tggaggacct gaacctggag taggatgtgc 5160 tggaagagga attattactg ctattaattt tttagaagaa gaaggagctt atactgattt 5220 agattttgta tcttatgatg tattaggaga tgtagtatgt ggaggatttg ctatgcctat 5280 tagagaaaat aaagctcaag aaatttatat tgtatgttct ggagaaatga tggctatgta 5340 tgctgctaat aatattgcta gaggagtatt aaaatatgct catgctggag gagtaagatt 5400 aggaggatta atttgtaatt ctagaaaagt agatagagaa actgaattaa ttgaaaattt 5460 agctgctaga ttaaatactc aaatgattca ttttgtacct agagataatg tagtacaaag 5520 agctgaaatt agaagaatga ctgtagaaca atatgctcct gaagataatc aagctcaaga 5580 atatgatcaa ttagctcaaa aaattattaa taatgaaaaa ttaactattc ctactccttt 5640 agaaatggat gaattagaag aattattaat tgaatttgga ttattaggag atgaagaaga 5700 tagacaaaaa caaattgctg ctcaagatgc tgctattaaa gctactgctg ctaaataagg 5760 atccacctga tctagagtcc gcaaaaatca ccagtctctc tctacaaatc tatctctctc 5820 tatttttctc cagaataatg tgtgagtagt tcccagataa gggaattagg gttcttatag 5880 ggtttcgctc atgtgttgag catataagaa acccttagta tgtatttgta tttgtaaaat 5940 acttctatca ataaaatttc taattcctaa aaccaaaatc cagtgacgcg gccgcaccca 6000 taatacccat aatagctgtt tgccatcgct accttaggac cgttatagtt aaccggtcaa 6060 catgtggagc acgacacact tgtctactcc aaaaatatca aagatacagt ctcagaagac 6120 caaagggcaa ttgagacttt tcaacaaagg gtaatatccg gaaacctcct cggattccat 6180 tgcccagcta tctgtcactt tattgtgaag atagtggaaa aggaaggtgg ctcctacaaa 6240 tgccatcatt gcgataaagg aaaggccatc gttgaagatg cctctgccga cagtggtccc 6300 aaagatggac ccccacccac gaggagcatc gtggaaaaag aagacgttcc aaccacgtct 6360 tcaaagcaag tggattgatg tgataacatg gtggagcacg acacacttgt ctactccaaa 6420 aatatcaaag atacagtctc agaagaccaa agggcaattg agacttttca acaaagggta 6480 atatccggaa acctcctcgg attccattgc ccagctatct gtcactttat tgtgaagata 6540 gtggaaaagg aaggtggctc ctacaaatgc catcattgcg ataaaggaaa ggccatcgtt 6600 gaagatgcct ctgccgacag tggtcccaaa gatggacccc cacccacgag gagcatcgtg 6660 gaaaaagaag acgttccaac cacgtcttca aagcaagtgg attgatgtga tatctccact 6720 gacgtaaggg atgacgcaca atcccactat ccttcgcaag acccttcctc tatataagga 6780 agttcatttc atttggagag gacgtcgaga gttctcaaca caacatatac aaaacaaacg 6840 aatctcaagc aatcaagcat tctacttcta ttgcagcaat ttaaatcatt tcttttaaag 6900 caaaagcaat tttctgaaaa ttttcaccat ttacgaacga tagccatggc tagcgctacc 6960 ggactcagat ctatggcttc ctctgtcatt tcttcagcag ctgttgccac acgcagcaat 7020 gttacacaag ctagcatggt tgcacctttc actggtctca aatcttcagc cactttccct 7080 gttacaaaga agcaaaacct tgacatcact tccattgcta gcaatggtgg aagagttagc 7140 tgcagagctc aaatggcttc tgaaaaaatt gaacaaaata aacaattaat tcaagaagta 7200 ttagatgctt atcctgctaa agctgctaaa agaagaaaaa aacatttaaa tgtaattgaa 7260 gaaaaaggag ctgattgtgg agtaaaatct aatgtaaaat ctgtacctgg agtaatgact 7320 actagaggat gtgcttttgc tggagctaaa ggagtagtat ggggacctgt aaaagatatg 7380 gtacatattt ctcatggacc tgtaggatgt ggatattatt cttgggctgg aagaagaaat 7440 tattataatg gagtaactgg agtagatact tttggaacta tgcaatttac ttctgatttt 7500 caagaaagag atattgtatt tggaggagat aaaaaattag ctaaaattat gaatgaaatt 7560 gaagaattat ttcctttaaa tgctggaatt actattgaat ctgaatgtcc tgtaggatta 7620 attggagatg atattgaagc tgtagctaaa aaagcttcta aagaattaaa taaacctgta 7680 gtacctgtaa gatgtgaagg atttagagga gtatctcaat ctttaggaca tcatattgct 7740 aatgatactg taagagattg ggtatatgaa ccttctgcta aagtaactaa tgaagaaatt 7800 ggatttgaaa aaactcctta tgatgtatct ttaattgctg attataatat tggaggagat 7860 ggatggtctt ctagattatt attagatgaa attggattaa gagtagtatc tcaagctact 7920 ggagatggaa cttataatga agtatttatg gctcctagag taaatttaaa tttaattcat 7980 tgttatagat ctatgaatta tatttgtaga tatatggaag aagaatatgg aattccttgg 8040 gtagaattta atttttttgg accttctcaa attgctaaat ctttaagaaa aattgcttct 8100 ttttttgatg ataaaattaa agaaaatact gaaaaagtaa ttgctagata tcaagaacaa 8160 gctgatgctg taattgctaa atatagacct agattagaag gaaaaaaagt aatgatgatg 8220 gtaggaggat taagacctag acatattatt cctgcttttg atgatttagg aatggaagta 8280 attggaactg gatatgaatt tggacatgga gatgattata aaagaactgc tgattatgct 8340 caagaaggaa ctttaattta tgatgatgta tctggatatg aatttgaaga atttgctaaa 8400 aaattaaaac ctgatttaat tgcttctgga attaaagaaa aatatgtatt tcaaaaaatg 8460 ggaatgcctt ttagacaaat gcattcttgg gattattctg gaccttatca tggatatgat 8520 ggatttgcta tttttgctag agatatggat ttagctttaa attctcctac ttggaattta 8580 attaaagctc cttggaaaca agctaaatag gatccacctg atctagagtc cgcaaaaatc 8640 accagtctct ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag 8700 ttcccagata agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga 8760 aacccttagt atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta 8820 aaaccaaaat ccagtgacgc ggccgctcgc taccttagga ccgttatagt taattaccct 8880 gttatcccta accggtcaac atgtggagca cgacacactt gtctactcca aaaatatcaa 8940 agatacagtc tcagaagacc aaagggcaat tgagactttt caacaaaggg taatatccgg 9000 aaacctcctc ggattccatt gcccagctat ctgtcacttt attgtgaaga tagtggaaaa 9060 ggaaggtggc tcctacaaat gccatcattg cgataaagga aaggccatcg ttgaagatgc 9120 ctctgccgac agtggtccca aagatggacc cccacccacg aggagcatcg tggaaaaaga 9180 agacgttcca accacgtctt caaagcaagt ggattgatgt gataacatgg tggagcacga 9240 cacacttgtc tactccaaaa atatcaaaga tacagtctca gaagaccaaa gggcaattga 9300 gacttttcaa caaagggtaa tatccggaaa cctcctcgga ttccattgcc cagctatctg 9360 tcactttatt gtgaagatag tggaaaagga aggtggctcc tacaaatgcc atcattgcga 9420 taaaggaaag gccatcgttg aagatgcctc tgccgacagt ggtcccaaag atggaccccc 9480 acccacgagg agcatcgtgg aaaaagaaga cgttccaacc acgtcttcaa agcaagtgga 9540 ttgatgtgat atctccactg acgtaaggga tgacgcacaa tcccactatc cttcgcaaga 9600 cccttcctct atataaggaa gttcatttca tttggagagg acgtcgagag ttctcaacac 9660 aacatataca aaacaaacga atctcaagca atcaagcatt ctacttctat tgcagcaatt 9720 taaatcattt cttttaaagc aaaagcaatt ttctgaaaat tttcaccatt tacgaacgat 9780 agccatggct agcgctaccg gactcagatc tatggcttcc tctgtcattt cttcagcagc 9840 tgttgccaca cgcagcaatg ttacacaagc tagcatggtt gcacctttca ctggtctcaa 9900 atcttcagcc actttccctg ttacaaagaa gcaaaacctt gacatcactt ccattgctag 9960 caatggtgga agagttagct gcagagctca aatgtctcaa aatgtagata aaattaaaga 10020 tcattttcaa ttatttcaag aacctgaata tcaagaaatg tttgctagaa aaagagaatt 10080 tgaaggagga gcttctaaag aagaaattga aagagtaaga gaatggacta aatcttggga 10140 atatagagaa aaaaattttg ctagagaagc tttaactatt aatcctgcta aagcttgtca 10200 acctttagga gctatttttg ctgctgctgg atttgaagga actttacctt ttgtacatgg 10260 atctcaagga tgtgtagctt attttagatc tcatttaact agaaattata aagaaccttt 10320 tcaagctgta tcttcttcta tgactgaaga tgctgctgta tttggaggat taaaaaatat 10380 gattgatgga ttagctaatt cttatgcttt atataaacct aaaatgattg ctttatgtac 10440 tacttgtatg gctgaagtaa ttggagatga tttaggatct tttattacta attctaaaaa 10500 tgaaggagct gtacctcaag attttcctgt accttttgct catactcctt cttttgtagg 10560 atctcatatt actggatatg ataatatgtt aaaaggaatt ttaattgctt taactgatgg 10620 aaaaaaaact gaaactgata atggaaaaat taattttatt cctggatttg atccttatat 10680 tggaaatatt agagatttaa aaaatatttt atctttaatg gatgtacctt ctactgtatt 10740 agctgataat gctgaatctt ttgattctcc taatttagga gaatttaaaa tgtataatgg 10800 aggaactact ttagaagaag ctggagattc tattaatgct aaagctacta tttcttttca 10860 aaaatattct actcctaaaa ctttagaata tttaaaacaa gaaggaggac aaaaaactgc 10920 tacttataga cctattggag taagaggaac tgatgaattt ttaatggctt tatctgaatt 10980 aactggaaaa gctattcctg aagaattaga aattgaaaga ggaagagtag tagatgctat 11040 tactgattct caagcttggt tacatggaaa aagaattgct atttatggag atcctgatca 11100 tgtattagga ttattaaatt ttactttaga attaggaatg caacctgtac atgtagtagt 11160 aaataatgga aatgtagctg gatttgaaga agaagctaaa gaattattag ctaatgatcc 11220 taatggaaaa gaagctactg tatggattgg aaaagattta tggcatttaa gatctttatt 11280 agatactgaa cctgtagatt tattaattgg aaattcttat ggaaaatttt tacaaagaga 11340 tactggaact cctttagtaa gaattggata tcctattttt gatagacatc atcaacatag 11400 atattctatt ttaggatata aaggagcttt taatttaatt aattggattg taaatactat 11460 tttagatgaa ttagatagag gatctatgga tttaggagta aatgatactt cttttgattt 11520 agtaagataa ggatccacct gatctagagt ccgcaaaaat caccagtctc tctctacaaa 11580 tctatctctc tctatttttc tccagaataa tgtgtgagta gttcccagat aagggaatta 11640 gggttcttat agggtttcgc tcatgtgttg agcatataag aaacccttag tatgtatttg 11700 tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa tccagtgacg 11760 cggccgcatt accctgttat ccctattaat taagagctcg ctaccttaag agaggatatc 11820 ggcgcgcctc tagaatttaa atggatccta cgtactcgag gaattcaatt cggcgttaat 11880 tcagtacatt aaaaacgtcc gcaatgtgtt attaagttgt ctaagcgtca atttgtttac 11940 accacaatat atcctgccac cagccagcca acagctcccc gaccggcagc tcggcacaaa 12000 atcaccactc gatacaggca gcccatcagt ccgggacggc gtcagcggga gagccgttgt 12060 aaggcggcag actttgctca tgttaccgat gctattcgga agaacggcaa ctaagctgcc 12120 gggtttgaaa cacggatgat ctcgcggagg gtagcatgtt gattgtaacg atgacagagc 12180 gttgctgcct gtgatcaatt cgggcacgaa cccagtggac ataagcctcg ttcggttcgt 12240 aagctgtaat gcaagtagcg taactgccgt cacgcaactg gtccagaacc ttgaccgaac 12300 gcagcggtgg taacggcgca gtggcggttt tcatggcttc ttgttatgac atgttttttt 12360 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt 12420 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta 12480 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc 12540 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg 12600 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt 12660 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga 12720 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg 12780 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt 12840 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca 12900 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct 12960 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac 13020 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta 13080 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc 13140 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc 13200 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc 13260 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgccgg 13320 cgttaactca agcgattaga tgcactaagc acataattgc tcacagccaa actatcaggt 13380 caagtctgct tttattattt ttaagcgtgc ataataagcc ctacacaaat tgggagatat 13440 atcatgcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt 13500 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca 13560 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct 13620 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta 13680 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct 13740 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc 13800 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca 13860 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga 13920 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg 13980 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt 14040 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag 14100 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt 14160 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt 14220 tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga 14280 ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca 14340 ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac 14400 actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct 14460 gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc 14520 tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagggt 14580 gccttgatgt gggcgccggc ggtcgagtgg cgacggcgcg gcttgtccgc gccctggtag 14640 attgcctggc cgtaggccag ccatttttga gcggccagcg gccgcgatag gccgacgcga 14700 agcggcgggg cgtagggagc gcagcgaccg aagggtaggc gctttttgca gctcttcggc 14760 tgtgcgctgg ccagacagtt atgcacaggc caggcgggtt ttaagagttt taataagttt 14820 taaagagttt taggcggaaa aatcgccttt tttctctttt atatcagtca cttacatgtg 14880 tgaccggttc ccaatgtacg gctttgggtt cccaatgtac gggttccggt tcccaatgta 14940 cggctttggg ttcccaatgt acgtgctatc cacaggaaag agaccttttc gacctttttc 15000 ccctgctagg gcaatttgcc ctagcatctg ctccgtacat taggaaccgg cggatgcttc 15060 gccctcgatc aggttgcggt agcgcatgac taggatcggg ccagcctgcc ccgcctcctc 15120 cttcaaatcg tactccggca ggtcatttga cccgatcagc ttgcgcacgg tgaaacagaa 15180 cttcttgaac tctccggcgc tgccactgcg ttcgtagatc gtcttgaaca accatctggc 15240 ttctgccttg cctgcggcgc ggcgtgccag gcggtagaga aaacggccga tgccgggatc 15300 gatcaaaaag taatcggggt gaaccgtcag cacgtccggg ttcttgcctt ctgtgatctc 15360 gcggtacatc caatcagcta gctcgatctc gatgtactcc ggccgcccgg tttcgctctt 15420 tacgatcttg tagcggctaa tcaaggcttc accctcggat accgtcacca ggcggccgtt 15480 cttggccttc ttcgtacgct gcatggcaac gtgcgtggtg tttaaccgaa tgcaggtttc 15540 taccaggtcg tctttctgct ttccgccatc ggctcgccgg cagaacttga gtacgtccgc 15600 aacgtgtgga cggaacacgc ggccgggctt gtctcccttc ccttcccggt atcggttcat 15660 ggattcggtt agatgggaaa ccgccatcag taccaggtcg taatcccaca cactggccat 15720 gccggccggc cctgcggaaa cctctacgtg cccgtctgga agctcgtagc ggatcacctc 15780 gccagctcgt cggtcacgct tcgacagacg gaaaacggcc acgtccatga tgctgcgact 15840 atcgcgggtg cccacgtcat agagcatcgg aacgaaaaaa tctggttgct cgtcgccctt 15900 gggcggcttc ctaatcgacg gcgcaccggc tgccggcggt tgccgggatt ctttgcggat 15960 tcgatcagcg gccgcttgcc acgattcacc ggggcgtgct tctgcctcga tgcgttgccg 16020 ctgggcggcc tgcgcggcct tcaacttctc caccaggtca tcacccagcg ccgcgccgat 16080 ttgtaccggg ccggatggtt tgcgaccgtc acgccgattc ctcgggcttg ggggttccag 16140 tgccattgca gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc 16200 cacacatggg gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc 16260 ctttagccgc taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc 16320 gcgatgtatt cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc 16380 agcttggtgt gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct 16440 gccaggctgg ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc 16500 gtgtttgtgc ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa 16560 tttcagcggc cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa 16620 cggttgtgcc ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa 16680 gaatgggcag ctcgtacccg gccagcgcct cggcaacctc accgccgt 16728 <210> 48 <211> 12181 <212> DNA <213> Artificial Sequence <220> <223> Plasmid DNA host by E.Coli Xl1B <400> 48 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 60 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 120 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 180 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 240 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 300 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 360 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 420 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtacccg 660 attggggcgt ggacataagg gtctttatga cacaatcaac aattcgcttc attttcaatt 720 aggccttgct ctagcttctt taggggttat tacttctttg gtagctcaac acatgtactc 780 tttacctgct tatgcattca tagcacaaga ctttactact caagctgcat tatataccca 840 ccaccaatat atcgcaggat tcatcatgac aggagctttt gctcatggag ctatattttt 900 cattagagat tacaatccgg agcaaaatga agataatgta ttggcaagaa tgttagatca 960 taaagaagct atcatatctc atttaagttg ggccagcctc tttctgggat tccataccct 1020 gggactttat gttcataatg atgtcatgct tgcctttggc actccggaga agcaaatctt 1080 gattgaaccg atatttgctc aatggataca atccgctcat ggtaaaactt catatgggtt 1140 cgatgtactt ttatcttcaa cgactggccc agcattcaat gcgggtcgaa gcatctggtt 1200 gccgggttgg ttaaatgctg ttaatgaaaa tagtaattca ttattcttaa caataggtcc 1260 tggagacttt ttggttcatc atgctattgc ccttggttta catacaacta cattgatctt 1320 agtaaaaggt gctttagatg cacgtggttc caagttaatg ccagataaaa aggatttcgg 1380 ttatagtttt ccgtgcgatg gcccaggacg aggcggtact tgtgatattt cggcatggga 1440 cgcgttttat ttggcagttt tttggatgtt aaatactatt ggatgggtta ctttttattg 1500 gcattggaag cacatcacat tatggcaagg taacgtttca cagtttaatg aatcttccac 1560 ttatttgatg ggctggttaa gggattattt atggttaaac tcttcacaac ttatcaatgg 1620 atataaccct tttggtatga atagtttatc ggtttgggca tggatgttct tatttggaca 1680 tcttgtttgg gctactggat ttatgttctt aatttcttgg cgtggatatt ggcaggaatt 1740 gattgaaact ttagcatggg ctcacgaacg cacacctttg gccaatttga ttcgatggag 1800 agataaacca gtggcccttt ctattgtaca agcaagattg gttggattag ctcacttttc 1860 tgtaggttat atattcactt atgcggcttt cttgattgcc tctacgtcgg gcaaatttgg 1920 ttaattaatg tgtgtattcg cgataatctc atttctttcg acggagaagg gggtccacct 1980 tcttctattt ctacatctag gattcgactt gtatcatgga tactaatagg aattcaacca 2040 ttatggcaag gaaaagtttg attcagaggg agaagaagag gcaaaaattg gaacagaaat 2100 atcattcgat tcgtcgatcc tcaaaaaaag aaataagcaa ggttccgtcg ttgagtgaca 2160 aatgggaaat ttatggaaag ttacaatccc taccacggaa tagtgcacct acacgccttc 2220 atcgacgttg ttttttgacc ggaaggccga gagctaacta tcgagacttt ggcctatccg 2280 gacacatact tcgtgaaatg gttcatgcat gtttgttgcc aggagcaaca agatcaagtt 2340 ggtaaggatt aacgcttcat ttctatttct atggtcgatg atcatagaag cccctttacc 2400 attctgtata aatgggctat tctatttgta cagatagggt ggaggggcgc atttaatcct 2460 tgtttatcta ttagttttca gttcttatct ttggcgcggg gtagagcagt ttggtagctc 2520 gcaaggctca taaccttgag gtcacgggtt caaatcctgt ctccgcaaca tcttattctg 2580 gtctcgaggt ccgcataaag aaccacccat aatacccata atagctgttt gccaaccggt 2640 cgccgtcgtt caatgagaat ggataagagg ctcgtgggat tgacgtgagg gggcagggat 2700 gactatattt ctgggagcga actccgggcg aatatgaagc gcatggatac aagttatgcc 2760 ttggaatgaa agacaattcc gaatccgctt tgtctacgaa caaggaagct ataagtaatg 2820 caactatgaa ggatctatgt ctatcgataa gaaaatccgt caaatcgcat tttacggaaa 2880 aggcggtatc ggaaaatcca ctacctcaca aaacactctt gctgcaatgg ctgaaatggg 2940 tcaaagaatc cttatcgttg gatgcgatcc taaagcagac agtacccgcc ttatgcttca 3000 ctctaaagct caaaccaccg tacttcatct tgcagctgaa cgtggtgctg ttgaagatct 3060 tgaattagag gaagtaatgt taactggttt tagaggagtt aaatgcgtag agtctggtgg 3120 tccagaacca ggtgttggat gtgcaggtcg aggaatcatc actgctatca atttcttaga 3180 ggaaaacggt gcatatcaag atgtagattt tgtttcatac gatgttttag gtgatgtagt 3240 ttgtggtgga ttcgctatgc cgatccgtga gaacaaagca caagaaatct atatcgtaac 3300 cagtggtgag atgatggcta tgtacgcagc taataacatc gctagaggta tcttaaagta 3360 tgcacatact ggaggggttc gattaggcgg gttaatctgt aattcacgta acgtagatcg 3420 tgaaatcgag ttgatcgaaa ctttggctaa aagattgaat actcaaatga tccattacgt 3480 tcctcgcgat aacatcgtac aacatgccga gttgcgtcgc atgacagtaa atgaatatgc 3540 ccctgattcc aatcagtcta atgagtatcg tatcttggcg aataaaatca tcaataatga 3600 aaatttgaaa gtacctactc ctatcgagat ggaagaacta gaagaactac tgatcgaatt 3660 tggtatcctg gaatctgaag aaaatgctgc taaaatgatc gggactcctg ctcagtcttc 3720 cactaaataa tagcggccgc aacccataat acccataata gctgtttgcc atcgctacct 3780 taggaccgtt atagttaacc ggtggaggca gactatgact cctccagaaa atcaaaacct 3840 agtagatgag aataaagagt taatcaaaga agtgcttcaa gcttaccctg aaaaggcacg 3900 aaaaaaaaga gagaagcatt taaatgttca cgaagaaagt aaatccgatt gtggtgtaaa 3960 atctaacata aagtccattc caggcgttat gactgctcgt gggtgcgcat atgctggtag 4020 caaaggggta gtttggggtc ctatcaaaga tatgattcat atatcccacg ggcctgtagg 4080 ttgtgggtac tggtcttggt ccggaagacg taattattac gttggtatca ccggaattaa 4140 ctctttcggt actatgcatt ttacttccga tttccaggaa agagacatcg tatttggagg 4200 tgataagaaa ttgaccaaaa taattgatga gttagaaatc ctttttccat taaatcgtgg 4260 agtgtctatt caatcagaat gccctatcgg tttgattgga ggtgatatcg aagcagttgc 4320 taaaaaagca tctaagcagt atggaaaacc agtagttcct ttaagatgtg agggtctgcg 4380 tggagtatca caatctttag gtcatcacat tgctaacgac gcaatcagag attggatttt 4440 cccagaattt gataaggcta aaaaaaataa tactatcgac tttgaacctt caccgtacga 4500 tgttgcactt attggagatc ataacatcgg tggagatgct tgggcatctc gtatgttatt 4560 ggaggaaatg ggtctgagag tagttgctca atggtcagga gatggtactt taaatgaact 4620 tattcaagga cctgcagcta aattggtact gattcactgc tatcgttcta tgaattacat 4680 ctgtcgctca ttggaggaac aatatggtat gccgtggatg gaattcaact tttttggacc 4740 tactaaaatt gcagcttctc ttcgtgagat cgcagctaaa ttcgacagta aaattcaaga 4800 aaatgcagaa aaagttatcg ctaaatacac cccggtaatg aatgcagtgc ttgagaaata 4860 tcgccctcgt ttggaaggta acactgttat gctttacgta gggggtcttc gccctcgtca 4920 tgttgtacct gctttcgaag atttggggat taaagttgta ggtaccgggt atgaatttgc 4980 acataatgat gattacaaac gcactacaca ctatatcgat aacgctacta ttatctacga 5040 tgatgttaca gcctatgagt tcgaagaatt tgtaaaagct aaaaaacctg atcttattgc 5100 ctctggtatc aaagaaaaat acgcttttca gaaaatgggt ctaccttttc gacaaatgca 5160 tagttgggat tattctggtc cttatcatgg ttatgatcgt tttgccatct ttgctcgaga 5220 tatggatctt gccctaaata gtcctacttg gagtctaatc ggtgcgcctt ggaaaaaagc 5280 ggctactaaa gcgaaagctg ctgcttaata actcgagaga tacaacaatg cctcaaaatc 5340 cagagaaaat tgtagatcat gtacaattat ttcagcaacc tgaatatcaa gaacttttca 5400 aaaataaaca cgaacaattt gagggagctc attccccaga agaagtacag agagtatccg 5460 aatggactaa aggttgggag tacagagaaa agaactttgc acgtcaagct ttaacagtaa 5520 atcctgcaaa aggatgccaa ccagtaggtg ctatcttcgc agctgtagga tttgaaggta 5580 ctctaccttt cgtacaagga tcccagggtt gtgtagcata ttttagaaca catttatccc 5640 gtcactacaa agaaccattc tctgctgtat cctcatctat gactgaggat gcagctgtat 5700 ttggaggtct taacaatatg attgaaggat tacaagtatc atatcaacta tacaaaccta 5760 aaatgatcgc agtatgtaca acttgtatgg ctgaagtaat tggtgatgat ttaggagcat 5820 tcatcacaaa tgctaaaaac gcaggttcta ttccacaaga ttttcctgta ccattcgctc 5880 atactccttc atttgtagga tctcatacaa ccggttatga taatatgatg aagggaatcc 5940 tttcaaattt aactgagggt aaaaaaaaag ataagaccaa cggaaaaatt aatttcatcc 6000 caggttttga tacttacgta ggaaataaca gagaactgaa acgtatgtta ggtcttatgg 6060 gaattgatta taccatctta gcagataatt ctgattacct ggatgctcct aatactggtg 6120 aatatgatat gtacccgggc ggtaccaaat tagaggatgc agctgattca tgcaacgcag 6180 aagctactgt agcccttcag agatatacca ctaccaagac tcgagaatac attgagacca 6240 aatggaaaca acaaactaaa gtattacgtc ctttcggggt aaaaggtacc gacgaatttc 6300 ttatgacttt atctgaaatg accgggaaac cgatccctca agaacttgag gatgaacgag 6360 gtcgtttagt agacgctatg actgatagtt atgcctggat tcacgggaaa aaattcgcta 6420 tctacggtga tccggacctt atttattctg taaccagttt tttacttgaa ttaggggccg 6480 aacctgtaca tatcctttgt aataatggtg atgaagagtt caaaaaagag atggaagcta 6540 ttttatctgc gagtccgttt gggaaacaag ctactgtatg gatccagaaa gatttgtggc 6600 atcttcgatc tttgcttttt actgaacctg tagatttttt tgtaggtaac agttacggga 6660 aatatttgtg gcgcgacacc aaaattccta tggtacgtat cggttatcct ctttttgatc 6720 gccaccattt gcatcgttat cctactttgg ggtatcaagg tggtttgaat ttgcttaatt 6780 gggtagtaaa cactattttg gatgaacttg atcgcagtag caatatagcg ggtaaaactg 6840 atatatcttt tgatttgatc cgttaatagg cggccgctcg ctaccttagg accgttatag 6900 ttattaccct gttatcccta accggtggag gcttcttgtt atgacatgtt tttttggggt 6960 acagtctatg cctcgggcat ccaagcagca agcgcgttac gccgtgggtc gatgtttgat 7020 gttatggagc agcaacgatg ttacgcagca gggcagtcgc cctaaaacaa agttaaacat 7080 catgggggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat 7140 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg 7200 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga 7260 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag 7320 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg 7380 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg 7440 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga 7500 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca 7560 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc 7620 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg 7680 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta 7740 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc 7800 ctcgcgcgca gatcagttgg aagaatttgt ccactacgtg aaaggcgaga tcaccaaggt 7860 agtcggcaaa taatctcgag ctcaagcttc gaattctgca gtcgacggta ccgcgggccc 7920 gggatccacc tgatctagag tccgcaaaaa tcaccagtct ctctctacaa atctatctct 7980 ctctattttt ctccagaata atgtgtgagt agttcccaga taagggaatt agggttctta 8040 tagggtttcg ctcatgtgtt gagcatataa gaaaccctta gtatgtattt gtatttgtaa 8100 aatacttcta tcaataaaat ttctaattcc taaaaccaaa atccagtgac gcggccgcat 8160 taccctgtta tccctattaa ttaagagctc gctaccttaa gagaggatat cggcgcgccg 8220 atcctagcct agtctatagg aggttttgaa aagaaaggag caataatcat tttcttgttc 8280 tgtcaagagg gtgctattgc tcctttcttt tttttttttt ctttactaat ttcctagtat 8340 tttactgaca tagacttttt tgtttacatt atcgaaaaag aaagagaggg tatttgcttg 8400 catttattca tgatggatcc cccgggctgc aggcatgcaa gctaattccc gatctagtaa 8460 catagatgac accgcgcgcc gcggaactag taattaattc ccgcctttcg ctttttgggg 8520 gtggaaggca aaagaaaacg taggggaggg atagaatcac tacactatca cggccaacta 8580 taccaactcc ttaatgtaag gatatattta atgctattta tgaaattcaa taataaaaag 8640 aaatagtaaa aaaattactt tatcttggat cttgggcgga tagcgggaat cgaacccgca 8700 tcttctcctt ggcaaagaga aattttacca ttcgaccata tccgcatttt tttgttcttg 8760 atacacaata tgtacccaca tatatgatat ataaccggat cttatttgtg cagtgccggg 8820 acacatattc tcttcggaac gattccaata attttgttaa ttatattctt tttattcaag 8880 aagtttgacc cccctctaat ttttttgttt tctttatttg atttgcattt tctttgggga 8940 cttagattca aatttaatgt gtctcacaac cgagaaaaat taggggggtc attttggttt 9000 tgggtctgcg acgaataggt tcaagagatg agagaattaa ggatacccac cagaaagact 9060 aatccaatcc ataaggaggt accagaaaat acaacatttt tgttacttga ccagccatca 9120 ggagaagcaa atacaacggg tacgctaatc aataagatta atgaagtagc aattaatgca 9180 aaaacagcca attggaaagc aagagtcatg cttttaatcc tccaagctac caacaaatga 9240 actatatacc atttgatccc tctatcagcc aaaaaatatt aattgtgata aaatatgtca 9300 tcgagggatt ttactttatc atgaatccat tgattctata tgacttatta ctactccccc 9360 tttcgcactt tattcgtaca tggagtgggg tggggggaaa tggaattttc ttttttattt 9420 cacaaatgga catgctagat catatatcta tatacggata gatagatcga tcggcggatt 9480 cgcacctgag atctttctac agatagtggg ggtatccacc cctatagcca tgttctattc 9540 ggaggaataa aataaaaata gtctttcgga gagatggctg agtggttgat agccccggtc 9600 ttgaaaaccg gtatagtttt gaacaaagaa ctatcgaggg ttcgaatccc tctctctcct 9660 tttttgctaa ttgaatagat ttttttattt agtggttttg cccaatctgc tatccgaaag 9720 aaaagggaat ggctcggcta tcccacctag ccaagccaga aaaatagatt agatataaat 9780 tagataaaat aaatgagttg aaaaaaaaaa aagaaaaaag gaatacttaa gctgattcca 9840 agatgtatga ttgaatcaaa gtaatttgta cttcattcaa gcattggatc tcctgtctca 9900 tatcaattaa gaggggtcat ggaaagaaca ggttcaaagt cgcgatcaat tcctttttca 9960 aatcctgctg cagcgagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg 10020 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 10080 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 10140 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 10200 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 10260 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 10320 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 10380 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 10440 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 10500 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 10560 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 10620 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 10680 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 10740 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 10800 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 10860 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 10920 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 10980 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 11040 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 11100 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 11160 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 11220 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 11280 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 11340 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 11400 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 11460 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 11520 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 11580 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 11640 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 11700 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 11760 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 11820 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 11880 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 11940 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 12000 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 12060 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 12120 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 12180 c 12181 <210> 49 <211> 16791 <212> DNA <213> Artificial Sequence <220> <223> Plasmid DNA host by E.Coli Xl1B <400> 49 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga 60 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc 120 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg 180 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc 240 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg 300 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga 360 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt 420 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat 480 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag 540 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc 600 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa 660 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc 720 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc 780 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg 840 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc 900 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg 960 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc 1020 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg 1080 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg 1140 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg 1200 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca 1260 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc 1320 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg 1380 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc 1440 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag 1500 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca 1560 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc 1620 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg 1680 gataaacctt ttcacgccct tttaaatatc cgttattcta ataaacgctc ttttctctta 1740 ggtttacccg ccaatatatc ctgtcaaaca ctgatagttt aaactgaagg cgggaaacga 1800 caatctgatc caagctcaag ctaagcttca gagccaccac cctcagagcc gccaccagaa 1860 ccaccaccag agccgccgcc agcattgaca ggaggcccga tctagtaaca tagatgacac 1920 cgcgcgcgat aatttatcct agtttgcgcg ctatattttg ttttctatcg cgtattaaat 1980 gtataattgc gggactctaa tcataaaaac ccatctcata aataacgtca tgcattacat 2040 gttaattatt acatgcttaa cgtaattcaa cagaaattat atgataatca tcgcaagacc 2100 ggcaacagga ttcaatctta agaaacttta ttgccaaatg tttgaacgat cggggatcat 2160 ccgggtctgt ggcgggaact ccacgaaaat atccgaacgc agcaagatat cgcggtccat 2220 ctcggtcttg cctgggcagt cgccgccgac gccgttgatg tggacgccgg gcccgatcat 2280 attgtcgctc aggatcgtgg cgttgtgctt gtcggccgtt gctgtcgtaa tgatatcggc 2340 accttcgacc gcctgttccg cagagatccc gtgggcgaag aactccagca tgagatcccc 2400 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata 2460 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat 2520 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc 2580 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca 2640 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc 2700 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag 2760 caggcatcgc catgggtcac gacgagatca tcgccgtcgg gcatgcgcgc cttgagcctg 2820 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca 2880 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat 2940 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact 3000 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc 3060 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc 3120 gtggccagcc acgatagccg cgctgcctcg tcctgcagtt cattcagggc accggacagg 3180 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca 3240 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc 3300 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatccaga tccggtgcag 3360 attatttgga ttgagagtga atatgagact ctaattggat accgagggga atttatggaa 3420 cgtcagtgga gcatttttga caagaaatat ttgctagctg atagtgacct taggcgactt 3480 ttgaacgcgc aataatggtt tctgacgtat gtgcttagct cattaaactc cagaaacccg 3540 cggctgagtg gctccttcaa cgttgcggtt ctgtcagttc caaacgtaaa acggcttgtc 3600 ccgcgtcatc ggcgggggtc ataacgtgac tcccttaatt ctccgctcat gatcagattg 3660 tcgtttcccg ccttcaggta ccgcgatcgc tcgcgacctg caggcataaa gccgtcagtg 3720 tccgcataaa gaaccaaccc ataataccca taatagctgt ttgccaaccg gtcaacatgt 3780 ggagcacgac acacttgtct actccaaaaa tatcaaagat acagtctcag aagaccaaag 3840 ggcaattgag acttttcaac aaagggtaat atccggaaac ctcctcggat tccattgccc 3900 agctatctgt cactttattg tgaagatagt ggaaaaggaa ggtggctcct acaaatgcca 3960 tcattgcgat aaaggaaagg ccatcgttga agatgcctct gccgacagtg gtcccaaaga 4020 tggaccccca cccacgagga gcatcgtgga aaaagaagac gttccaacca cgtcttcaaa 4080 gcaagtggat tgatgtgata acatggtgga gcacgacaca cttgtctact ccaaaaatat 4140 caaagataca gtctcagaag accaaagggc aattgagact tttcaacaaa gggtaatatc 4200 cggaaacctc ctcggattcc attgcccagc tatctgtcac tttattgtga agatagtgga 4260 aaaggaaggt ggctcctaca aatgccatca ttgcgataaa ggaaaggcca tcgttgaaga 4320 tgcctctgcc gacagtggtc ccaaagatgg acccccaccc acgaggagca tcgtggaaaa 4380 agaagacgtt ccaaccacgt cttcaaagca agtggattga tgtgatatct ccactgacgt 4440 aagggatgac gcacaatccc actatccttc gcaagaccct tcctctatat aaggaagttc 4500 atttcatttg gagaggacgt cgagagttct caacacaaca tatacaaaac aaacgaatct 4560 caagcaatca agcattctac ttctattgca gcaatttaaa tcatttcttt taaagcaaaa 4620 gcaattttct gaaaattttc accatttacg aacgatagcc atggctagcg ctaccggact 4680 cagatctatg gcttcctctg tcatttcttc agcagctgtt gccacacgca gcaatgttac 4740 acaagctagc atggttgcac ctttcactgg tctcaaatct tcagccactt tccctgttac 4800 aaagaagcaa aaccttgaca tcacttccat tgctagcaat ggtggaagag ttagctgcag 4860 agctcaagct tcgaattcta tgtctatcga taagaaaatc cgtcaaatcg cattttacgg 4920 aaaaggcggt atcggaaaat ccactacctc acaaaacact cttgctgcaa tggctgaaat 4980 gggtcaaaga atccttatcg ttggatgcga tcctaaagca gacagtaccc gccttatgct 5040 tcactctaaa gctcaaacca ccgtacttca tcttgcagct gaacgtggtg ctgttgaaga 5100 tcttgaatta gaggaagtaa tgttaactgg ttttagagga gttaaatgcg tagagtctgg 5160 tggtccagaa ccaggtgttg gatgtgcagg tcgaggaatc atcactgcta tcaatttctt 5220 agaggaaaac ggtgcatatc aagatgtaga ttttgtttca tacgatgttt taggtgatgt 5280 agtttgtggt ggattcgcta tgccgatccg tgagaacaaa gcacaagaaa tctatatcgt 5340 aaccagtggt gagatgatgg ctatgtacgc agctaataac atcgctagag gtatcttaaa 5400 gtatgcacat actggagggg ttcgattagg cgggttaatc tgtaattcac gtaacgtaga 5460 tcgtgaaatc gagttgatcg aaactttggc taaaagattg aatactcaaa tgatccatta 5520 cgttcctcgc gataacatcg tacaacatgc cgagttgcgt cgcatgacag taaatgaata 5580 tgcccctgat tccaatcagt ctaatgagta tcgtatcttg gcgaataaaa tcatcaataa 5640 tgaaaatttg aaagtaccta ctcctatcga gatggaagaa ctagaagaac tactgatcga 5700 atttggtatc ctggaatctg aagaaaatgc tgctaaaatg atcgggactc ctgctcagtc 5760 ttccactaaa taaggatcca cctgatctag agtccgcaaa aatcaccagt ctctctctac 5820 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa 5880 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat 5940 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg 6000 acgcggccgc acccataata cccataatag ctgtttgcca tcgctacctt aggaccgtta 6060 tagttaaccg gtcaacatgt ggagcacgac acacttgtct actccaaaaa tatcaaagat 6120 acagtctcag aagaccaaag ggcaattgag acttttcaac aaagggtaat atccggaaac 6180 ctcctcggat tccattgccc agctatctgt cactttattg tgaagatagt ggaaaaggaa 6240 ggtggctcct acaaatgcca tcattgcgat aaaggaaagg ccatcgttga agatgcctct 6300 gccgacagtg gtcccaaaga tggaccccca cccacgagga gcatcgtgga aaaagaagac 6360 gttccaacca cgtcttcaaa gcaagtggat tgatgtgata acatggtgga gcacgacaca 6420 cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc aattgagact 6480 tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc tatctgtcac 6540 tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca ttgcgataaa 6600 ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg acccccaccc 6660 acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca agtggattga 6720 tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc gcaagaccct 6780 tcctctatat aaggaagttc atttcatttg gagaggacgt cgagagttct caacacaaca 6840 tatacaaaac aaacgaatct caagcaatca agcattctac ttctattgca gcaatttaaa 6900 tcatttcttt taaagcaaaa gcaattttct gaaaattttc accatttacg aacgatagcc 6960 atggctagcg ctaccggact cagatctatg gcttcctctg tcatttcttc agcagctgtt 7020 gccacacgca gcaatgttac acaagctagc atggttgcac ctttcactgg tctcaaatct 7080 tcagccactt tccctgttac aaagaagcaa aaccttgaca tcacttccat tgctagcaat 7140 ggtggaagag ttagctgcag agctcaaatg actcctccag aaaatcaaaa cctagtagat 7200 gagaataaag agttaatcaa agaagtgctt caagcttacc ctgaaaaggc acgaaaaaaa 7260 agagagaagc atttaaatgt tcacgaagaa agtaaatccg attgtggtgt aaaatctaac 7320 ataaagtcca ttccaggcgt tatgactgct cgtgggtgcg catatgctgg tagcaaaggg 7380 gtagtttggg gtcctatcaa agatatgatt catatatccc acgggcctgt aggttgtggg 7440 tactggtctt ggtccggaag acgtaattat tacgttggta tcaccggaat taactctttc 7500 ggtactatgc attttacttc cgatttccag gaaagagaca tcgtatttgg aggtgataag 7560 aaattgacca aaataattga tgagttagaa atcctttttc cattaaatcg tggagtgtct 7620 attcaatcag aatgccctat cggtttgatt ggaggtgata tcgaagcagt tgctaaaaaa 7680 gcatctaagc agtatggaaa accagtagtt cctttaagat gtgagggtct gcgtggagta 7740 tcacaatctt taggtcatca cattgctaac gacgcaatca gagattggat tttcccagaa 7800 tttgataagg ctaaaaaaaa taatactatc gactttgaac cttcaccgta cgatgttgca 7860 cttattggag atcataacat cggtggagat gcttgggcat ctcgtatgtt attggaggaa 7920 atgggtctga gagtagttgc tcaatggtca ggagatggta ctttaaatga acttattcaa 7980 ggacctgcag ctaaattggt actgattcac tgctatcgtt ctatgaatta catctgtcgc 8040 tcattggagg aacaatatgg tatgccgtgg atggaattca acttttttgg acctactaaa 8100 attgcagctt ctcttcgtga gatcgcagct aaattcgaca gtaaaattca agaaaatgca 8160 gaaaaagtta tcgctaaata caccccggta atgaatgcag tgcttgagaa atatcgccct 8220 cgtttggaag gtaacactgt tatgctttac gtagggggtc ttcgccctcg tcatgttgta 8280 cctgctttcg aagatttggg gattaaagtt gtaggtaccg ggtatgaatt tgcacataat 8340 gatgattaca aacgcactac acactatatc gataacgcta ctattatcta cgatgatgtt 8400 acagcctatg agttcgaaga atttgtaaaa gctaaaaaac ctgatcttat tgcctctggt 8460 atcaaagaaa aatacgcttt tcagaaaatg ggtctacctt ttcgacaaat gcatagttgg 8520 gattattctg gtccttatca tggttatgat cgttttgcca tctttgctcg agatatggat 8580 cttgccctaa atagtcctac ttggagtcta atcggtgcgc cttggaaaaa agcggctact 8640 aaagcgaaag ctgctgctta aggatccacc tgatctagag tccgcaaaaa tcaccagtct 8700 ctctctacaa atctatctct ctctattttt ctccagaata atgtgtgagt agttcccaga 8760 taagggaatt agggttctta tagggtttcg ctcatgtgtt gagcatataa gaaaccctta 8820 gtatgtattt gtatttgtaa aatacttcta tcaataaaat ttctaattcc taaaaccaaa 8880 atccagtgac gcggccgctc gctaccttag gaccgttata gttaattacc ctgttatccc 8940 taaccggtca acatgtggag cacgacacac ttgtctactc caaaaatatc aaagatacag 9000 tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc ggaaacctcc 9060 tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa aaggaaggtg 9120 gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat gcctctgccg 9180 acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa gaagacgttc 9240 caaccacgtc ttcaaagcaa gtggattgat gtgataacat ggtggagcac gacacacttg 9300 tctactccaa aaatatcaaa gatacagtct cagaagacca aagggcaatt gagacttttc 9360 aacaaagggt aatatccgga aacctcctcg gattccattg cccagctatc tgtcacttta 9420 ttgtgaagat agtggaaaag gaaggtggct cctacaaatg ccatcattgc gataaaggaa 9480 aggccatcgt tgaagatgcc tctgccgaca gtggtcccaa agatggaccc ccacccacga 9540 ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc aaagcaagtg gattgatgtg 9600 atatctccac tgacgtaagg gatgacgcac aatcccacta tccttcgcaa gacccttcct 9660 ctatataagg aagttcattt catttggaga ggacgtcgag agttctcaac acaacatata 9720 caaaacaaac gaatctcaag caatcaagca ttctacttct attgcagcaa tttaaatcat 9780 ttcttttaaa gcaaaagcaa ttttctgaaa attttcacca tttacgaacg atagccatgg 9840 ctagcgctac cggactcaga tctatggctt cctctgtcat ttcttcagca gctgttgcca 9900 cacgcagcaa tgttacacaa gctagcatgg ttgcaccttt cactggtctc aaatcttcag 9960 ccactttccc tgttacaaag aagcaaaacc ttgacatcac ttccattgct agcaatggtg 10020 gaagagttag ctgcagagct caagcttcga attctatgcc tcaaaatcca gagaaaattg 10080 tagatcatgt acaattattt cagcaacctg aatatcaaga acttttcaaa aataaacacg 10140 aacaatttga gggagctcat tccccagaag aagtacagag agtatccgaa tggactaaag 10200 gttgggagta cagagaaaag aactttgcac gtcaagcttt aacagtaaat cctgcaaaag 10260 gatgccaacc agtaggtgct atcttcgcag ctgtaggatt tgaaggtact ctacctttcg 10320 tacaaggatc ccagggttgt gtagcatatt ttagaacaca tttatcccgt cactacaaag 10380 aaccattctc tgctgtatcc tcatctatga ctgaggatgc agctgtattt ggaggtctta 10440 acaatatgat tgaaggatta caagtatcat atcaactata caaacctaaa atgatcgcag 10500 tatgtacaac ttgtatggct gaagtaattg gtgatgattt aggagcattc atcacaaatg 10560 ctaaaaacgc aggttctatt ccacaagatt ttcctgtacc attcgctcat actccttcat 10620 ttgtaggatc tcatacaacc ggttatgata atatgatgaa gggaatcctt tcaaatttaa 10680 ctgagggtaa aaaaaaagat aagaccaacg gaaaaattaa tttcatccca ggttttgata 10740 cttacgtagg aaataacaga gaactgaaac gtatgttagg tcttatggga attgattata 10800 ccatcttagc agataattct gattacctgg atgctcctaa tactggtgaa tatgatatgt 10860 acccgggcgg taccaaatta gaggatgcag ctgattcatg caacgcagaa gctactgtag 10920 cccttcagag atataccact accaagactc gagaatacat tgagaccaaa tggaaacaac 10980 aaactaaagt attacgtcct ttcggggtaa aaggtaccga cgaatttctt atgactttat 11040 ctgaaatgac cgggaaaccg atccctcaag aacttgagga tgaacgaggt cgtttagtag 11100 acgctatgac tgatagttat gcctggattc acgggaaaaa attcgctatc tacggtgatc 11160 cggaccttat ttattctgta accagttttt tacttgaatt aggggccgaa cctgtacata 11220 tcctttgtaa taatggtgat gaagagttca aaaaagagat ggaagctatt ttatctgcga 11280 gtccgtttgg gaaacaagct actgtatgga tccagaaaga tttgtggcat cttcgatctt 11340 tgctttttac tgaacctgta gatttttttg taggtaacag ttacgggaaa tatttgtggc 11400 gcgacaccaa aattcctatg gtacgtatcg gttatcctct ttttgatcgc caccatttgc 11460 atcgttatcc tactttgggg tatcaaggtg gtttgaattt gcttaattgg gtagtaaaca 11520 ctattttgga tgaacttgat cgcagtagca atatagcggg taaaactgat atatcttttg 11580 atttgatccg ttaagatcca cctgatctag agtccgcaaa aatcaccagt ctctctctac 11640 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa 11700 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat 11760 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg 11820 acgcggccgc attaccctgt tatccctatt aattaagagc tcgctacctt aagagaggat 11880 atcggcgcgc ctctagaatt taaatggatc ctacgtactc gaggaattca attcggcgtt 11940 aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg tcaatttgtt 12000 tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc agctcggcac 12060 aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg ggagagccgt 12120 tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg caactaagct 12180 gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta acgatgacag 12240 agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc tcgttcggtt 12300 cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga accttgaccg 12360 aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat gacatgtttt 12420 tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc cgtgggtcga 12480 tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc taaaacaaag 12540 ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc agaggtagtt 12600 ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta cggctccgca 12660 gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt gaccgtaagg 12720 cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc ggcttcccct 12780 ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga cgacatcatt 12840 ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg caatgacatt 12900 cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt gctgacaaaa 12960 gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt tgatccggtt 13020 cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa ctcgccgccc 13080 gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg gtacagcgca 13140 gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga gcgcctgccg 13200 gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca agaagaagat 13260 cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa aggcgagatc 13320 accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac gccgcttcgc 13380 cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc caaactatca 13440 ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga 13500 tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 13560 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 13620 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 13680 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt 13740 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 13800 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 13860 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 13920 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 13980 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 14040 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 14100 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 14160 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 14220 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc 14280 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag 14340 cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc 14400 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta 14460 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc 14520 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc 14580 gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag 14640 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg 14700 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg 14760 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc 14820 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag 14880 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat 14940 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat 15000 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt 15060 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc 15120 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc 15180 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca 15240 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct 15300 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg 15360 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat 15420 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct 15480 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc 15540 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt 15600 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc 15660 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt 15720 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc 15780 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac 15840 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg 15900 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc 15960 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg 16020 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg 16080 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc 16140 gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc ttgggggttc 16200 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc 16260 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc 16320 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc 16380 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc 16440 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg 16500 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt 16560 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt 16620 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa 16680 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact 16740 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg t 16791

Claims (35)

1. 하나 이상의 식물 세포를 프로모터 서열 및 터미네이터 서열에 작동가능하게 연결된 Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계;
   식물 세포로부터 하나 이상의 식물을 재생시키는 단계; 및
   증진된 질소 고정을 나타내는, 식물 세포로부터 재배된, 하나 이상의 식물을 선택하는 단계
   를 포함하는 방법에 의해서 생성되며, 상기 하나 이상의 식물은 Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함하는, 증진된 질소 고정을 나타내는 트랜스제닉 식물.
2. Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함하는, 제 1 항의 트랜스제닉 식물의 종자, 줄기, 잎 또는 뿌리.
3. 제 1 항에 있어서, 트랜스제닉 식물은 카놀라, 옥수수, 쌀, 담배, 대두, 목화, 알파파, 토마토, 밀, 감자 및 보리로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
4. 제 3 항에 있어서, 트랜스제닉 식물은 토마토 또는 담배인 것을 특징으로 하는 트랜스제닉 식물.
5. Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함하는, 제 1 항의 트랜스제닉 식물의 자손.
6. Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함하는, 제 3 항의 식물의 자손.
7. Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함하는, 제 4 항의 식물의 자손.
8. 제 1 항에 있어서, 하나 이상의 식물을 선택하는 단계는 낮은 질소 투입 조건에서 하나 이상의 식물을 성장시키는 것을 포함하는 것을 특징으로 하는 트랜스제닉 식물.
9. 제 1 항에 있어서, 하나 이상의 세포와 접촉하는 Nif-H, Nif-D 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열은 시아노박테리아, 아조토박테라세아에, 리조비아 및 프랭키아로 구성되는 군으로부터 선택된 공급원으로부터 얻어진 것을 특징으로 하는 트랜스제닉 식물.
10. 제 1 항의 트랜스제닉 식물을 토양과 접촉시켜 두는 단계를 포함하며, 이로써 트랜스제닉 식물이 성장함에 따라 토양으로부터 질소가 사용되고, 이로써 토양에서 질소의 전체 농도를 감소시키는, 토양에서 질소의 전체 농도를 감소시키는 방법.
11. 트랜스제닉 식물을 생성하기 위해서 식물 세포를 SEQ. ID. NO. 1, SEQ. ID. NO. 2 및 SEQ. ID. NO. 3 중 하나를 암호화하는 재조합 핵산 서열과 접촉시키는 단계;
    식물 세포로부터 하나 이상의 트랜스제닉 식물을 재생시키는 단계; 및
    식물 세포로부터 재배된 하나 이상의 트랜스제닉 식물을 선택하는 단계
    를 포함하는 방법에 의해서 생성되며, 선택된 트랜스제닉 식물은 각각 증진된 질소 고정을 나타내고,
    선택된 하나 이상의 트랜스제닉 식물은 각각 SEQ. ID. NO. 1, SEQ. ID. NO. 2 또는 SEQ. ID. NO. 3을 암호화하는 재조합 핵산 서열을 포함하는, 증진된 질소 고정을 나타내는 트랜스제닉 식물.
12. SEQ. ID. NO. 1, SEQ. ID. NO. 2 및 SEQ. ID. NO. 3 중 하나를 암호화하는 재조합 핵산 서열을 포함하는, 제 11 항의 트랜스제닉 식물의 종자, 줄기, 잎 또는 뿌리.
13. 제 11 항에 있어서, 트랜스제닉 식물은 카놀라, 옥수수, 쌀, 담배, 대두, 목화, 알파파, 토마토, 밀, 감자 및 보리로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
14. 제 11 항의 방법에 따라서 생성된 트랜스제닉 식물로부터 유래된 식물-유래 상업적 제품으로서, 상기 트랜스제닉 식물은 SEQ. ID. NO. 1, SEQ. ID. NO. 2 및 SEQ. ID. NO. 3 중 하나를 암호화하는 재조합 핵산 서열을 포함하는 제품.
15. 제 14 항에 있어서, 상기 상업적 제품은 펄프, 종이, 종이 제품, 럼버, 시거렛, 시거, 씹는 담배, 빵, 밀가루, 시리얼, 오트밀, 또는 쌀인 것을 특징으로 하는 식물-유래 상업적 제품.
16. 제 11 항의 트랜스제닉 식물을 토양과 접촉시켜 두는 단계를 포함하며, 이로써 식물이 성장함에 따라 토양으로부터 질소가 사용되고, 이로써 토양에서 질소의 전체 농도를 감소시키는, 토양에서 질소의 전체 농도를 감소시키는 방법.
17. 트랜스제닉 식물을 생성하기 위해서 식물 세포를 SEQ. ID. NO. 1, SEQ. ID. NO. 2 및 SEQ. ID. NO. 3 중 하나를 암호화하는 재조합 핵산 서열과 접촉시키는 단계;
    식물 세포를 토마토 엽록체 발현에 대해 최적화된 적어도 하나의 합성 코딩 DNA 서열(sCDS)과 접촉시키는 단계;
    식물 세포로부터 하나 이상의 트랜스제닉 식물을 재생시키는 단계; 및
    증진된 질소 고정을 나타내는, 식물 세포로부터 재배된, 하나 이상의 트랜스제닉 식물을 선택하는 단계
    를 포함하는 방법에 의해서 생성되며, 상기 하나 이상의 식물은 SEQ. ID. No. 1, SEQ. ID. No. 2 또는 SEQ. ID. NO. 3을 암호화하는 재조합 핵산 서열과 sCDS를 포함하는, 증진된 질소 고정을 나타내는 트랜스제닉 식물.
18. 제 17 항에 있어서, 트랜스제닉 식물은 카놀라, 옥수수, 쌀, 담배, 대두, 목화, 알파파, 토마토, 밀, 감자 및 보리로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
19. 제 17 항에 있어서, sCDS는 SEQ. ID. NO. 29, SEQ. ID. NO. 30, SEQ. ID. NO. 31, SEQ. ID. NO. 32, SEQ. ID. NO. 33, SEQ. ID. NO. 34, SEQ. ID. NO 35, SEQ. ID. NO. 36, SEQ. ID. NO. 37, SEQ. ID. NO. 38, SEQ. ID. NO. 39 및 SEQ. ID. NO. 40으로 필수적으로 구성되는 군 중 하나를 포함하는 것을 특징으로 하는 트랜스제닉 식물.
20. 제 17 항의 트랜스제닉 식물을 토양과 접촉시켜 두는 단계를 포함하며, 이로써 식물이 성장함에 따라 토양으로부터 질소가 사용되고, 이로써 토양에서 질소의 전체 농도를 감소시키는, 토양에서 질소의 전체 농도를 감소시키는 방법.
21. 제 17 항의 트랜스제닉 식물을 토양과 접촉시켜 두는 단계를 포함하며, 이로써 식물이 붕괴함에 따라 식물이 토양으로 질소를 방출하는, 토양에서 질소 농도를 증가시키는 방법.
22. 트랜스제닉 식물을 생성하기 위해서 식물 세포를 SEQ. ID. NO. 46, SEQ. ID. NO. 47, SEQ. ID. NO. 48 및 SEQ. ID. NO. 49 중 하나를 암호화하는 재조합 핵산 서열과 접촉시키는 단계;
    식물 세포로부터 하나 이상의 트랜스제닉 식물을 재생시키는 단계; 및
    식물 세포로부터 재배된 하나 이상의 트랜스제닉 식물을 선택하는 단계
    를 포함하는 방법에 의해서 생성되며, 선택된 식물은 각각 증진된 질소 고정을 나타내고,
    선택된 하나 이상의 트랜스제닉 식물은 각각 SEQ. ID. NO. 46, SEQ. ID. NO. 47, SEQ. ID. NO. 48 또는 SEQ. ID. NO. 49를 암호화하는 재조합 핵산 서열을 포함하는, 증진된 질소 고정을 나타내는 트랜스제닉 식물.
23. SEQ. ID. NO. 46, SEQ. ID. NO. 47, SEQ. ID. NO. 48 및 SEQ. ID. NO. 49 중 하나를 암호화하는 재조합 핵산 서열을 포함하는, 제 22 항의 트랜스제닉 식물의 종자, 줄기, 잎 또는 뿌리.
24. 제 22 항에 있어서, 트랜스제닉 식물은 카놀라, 옥수수, 쌀, 담배, 대두, 목화, 알파파, 토마토, 밀, 감자 및 보리로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
25. 제 22 항의 방법에 따라서 생성된 트랜스제닉 식물로부터 유래된 트랜스제닉 식물-유래 상업적 제품으로서, 상기 트랜스제닉 식물은 SEQ. ID. NO. 46, SEQ. ID. NO. 47, SEQ. ID. NO. 48 및 SEQ. ID. NO. 49 중 하나를 암호화하는 재조합 핵산 서열을 포함하는 제품.
26. 제 25 항에 있어서, 상기 상업적 제품은 펄프, 종이, 종이 제품, 럼버, 시거렛, 시거, 씹는 담배, 빵, 밀가루, 시리얼, 오트밀, 또는 쌀인 것을 특징으로 하는 트랜스제닉 식물-유래 상업적 제품.
27. 제 22 항의 트랜스제닉 식물을 토양과 접촉시켜 두는 단계를 포함하며, 이로써 식물이 성장함에 따라 토양으로부터 질소가 사용되고, 이로써 토양에서 질소의 전체 농도를 감소시키는, 토양에서 질소의 전체 농도를 감소시키는 방법.
28. 하나 이상의 트랜스제닉 식물 세포를 제1 프로모터에 작동가능하게 연결된 Nif-H 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계;
    트랜스제닉 식물 세포를 제2 프로모터에 작동가능하게 연결된 Nif-D 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계;
    트랜스제닉 식물 세포를 제3 프로모터에 작동가능하게 연결된 Nif-K 유전자를 암호화하는 재조합 핵산 서열과 접촉시키는 단계;
    식물 세포로부터 하나 이상의 트랜스제닉 식물을 재생시키는 단계; 및
    식물 세포로부터 재배된 하나 이상의 트랜스제닉 식물을 선택하는 단계
    를 포함하는 방법에 의해서 생성되며, 선택된 트랜스제닉 식물은 각각 증진된 질소 고정을 나타내고, 선택된 트랜스제닉 식물은 각각 Nif-H 유전자를 암호화하는 재조합 핵산 서열, Nif-D 유전자를 암호화하는 재조합 핵산 서열 및 Nif-K 유전자를 암호화하는 재조합 핵산 서열을 포함하는, 증진된 질소 고정을 나타내는 트랜스제닉 식물.
29. 제 28 항에 있어서, Nif-H 유전자를 암호화하는 재조합 핵산 서열은 SEQ. ID. NOs. 29, 32, 35 및 38로 필수적으로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
30. 제 28 항에 있어서, Nif-D 유전자를 암호화하는 재조합 핵산 서열은 SEQ. ID. NOs. 30, 33, 36 및 39로 필수적으로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
31. 제 28 항에 있어서, Nif-K 유전자를 암호화하는 재조합 핵산 서열은 SEQ. ID. NOs. 31, 34, 37 및 40으로 필수적으로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
32. 제 28 항에 있어서, 제1 프로모터는 SEQ. ID. NOs. 41-45로 필수적으로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
33. 제 28 항에 있어서, 제2 프로모터는 SEQ. ID. NOs. 41-45로 필수적으로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
34. 제 28 항에 있어서, 제3 프로모터는 SEQ. ID. NOs. 41-45로 필수적으로 구성되는 군으로부터 선택되는 것을 특징으로 하는 트랜스제닉 식물.
35. 제 28 항에 있어서, 프로모터 중 적어도 하나는 SEQ. ID. NO. 4 또는 SEQ. ID. NO. 28을 포함하는 것을 특징으로 하는 트랜스제닉 식물.
    

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