JP6154479B2 - 選択マーカーを含まないクローン化された非ヒト動物 - Google Patents

選択マーカーを含まないクローン化された非ヒト動物 Download PDF

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Publication number
JP6154479B2
JP6154479B2 JP2015544195A JP2015544195A JP6154479B2 JP 6154479 B2 JP6154479 B2 JP 6154479B2 JP 2015544195 A JP2015544195 A JP 2015544195A JP 2015544195 A JP2015544195 A JP 2015544195A JP 6154479 B2 JP6154479 B2 JP 6154479B2
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promoter
gene
recombinase
site
selectable marker
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JP2016502407A (ja
JP2016502407A5 (enExample
Inventor
グオチュン ゴン，
グオチュン ゴン，
カ−マン ビーナス ライ，
カ−マン ビーナス ライ，
デイビッド エム． バレンズエラ，
デイビッド エム． バレンズエラ，
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Regeneron Pharmaceuticals Inc
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Regeneron Pharmaceuticals Inc
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0273—Cloned vertebrates
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
    • A61D19/00—Instruments or methods for reproduction or fertilisation
    • A61D19/04—Instruments or methods for reproduction or fertilisation for embryo transplantation
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8771—Bovine embryos
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/873—Techniques for producing new embryos, e.g. nuclear transfer, manipulation of totipotent cells or production of chimeric embryos
    • C12N15/877—Techniques for producing new mammalian cloned embryos
    • C12N15/8778—Swine embryos
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/203—Animal model comprising inducible/conditional expression system, e.g. hormones, tet
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/008—Vector systems having a special element relevant for transcription cell type or tissue specific enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/007—Vectors comprising a special translation-regulating system cell or tissue specific
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2999/00—Further aspects of viruses or vectors not covered by groups C12N2710/00 - C12N2796/00 or C12N2800/00
    • C12N2999/007—Technological advancements, e.g. new system for producing known virus, cre-lox system for production of transgenic animals

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Environmental Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Veterinary Medicine (AREA)
  • Wood Science & Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Public Health (AREA)
  • Reproductive Health (AREA)
  • Transplantation (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
JP2015544195A 2012-11-28 2013-11-26 選択マーカーを含まないクローン化された非ヒト動物 Expired - Fee Related JP6154479B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261730771P 2012-11-28 2012-11-28
US61/730,771 2012-11-28
PCT/US2013/072088 WO2014085501A1 (en) 2012-11-28 2013-11-26 Cloned non-human animals free of selective markers

Related Child Applications (1)

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JP2016226816A Division JP6227747B2 (ja) 2012-11-28 2016-11-22 選択マーカーを含まないクローン化された非ヒト動物

Publications (3)

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JP2016502407A JP2016502407A (ja) 2016-01-28
JP2016502407A5 JP2016502407A5 (enExample) 2017-01-12
JP6154479B2 true JP6154479B2 (ja) 2017-06-28

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JP2015544195A Expired - Fee Related JP6154479B2 (ja) 2012-11-28 2013-11-26 選択マーカーを含まないクローン化された非ヒト動物
JP2016226816A Expired - Fee Related JP6227747B2 (ja) 2012-11-28 2016-11-22 選択マーカーを含まないクローン化された非ヒト動物

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JP2016226816A Expired - Fee Related JP6227747B2 (ja) 2012-11-28 2016-11-22 選択マーカーを含まないクローン化された非ヒト動物

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Country Link
US (1) US20140221734A1 (enExample)
EP (1) EP2925122A1 (enExample)
JP (2) JP6154479B2 (enExample)
KR (1) KR20150089004A (enExample)
CN (1) CN104918484A (enExample)
AU (1) AU2013352235A1 (enExample)
BR (1) BR112015012408A2 (enExample)
CA (1) CA2891163A1 (enExample)
HK (1) HK1211177A1 (enExample)
IL (1) IL238771A0 (enExample)
MX (1) MX353279B (enExample)
MY (1) MY193790A (enExample)
RU (1) RU2015122802A (enExample)
SG (1) SG11201503752SA (enExample)
WO (1) WO2014085501A1 (enExample)
ZA (1) ZA201503305B (enExample)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3988649B1 (en) 2013-09-18 2024-11-27 Kymab Limited Methods, cells and organisms
JP6929527B2 (ja) * 2015-03-04 2021-09-01 株式会社ポル・メド・テック 安定した表現型を示す疾患モデルブタおよびその作製方法
CN105200086A (zh) * 2015-10-22 2015-12-30 中国农业科学院北京畜牧兽医研究所 一种无选择标记的多基因复合性状基因工程猪的制备方法
EP4249074A3 (en) * 2015-11-04 2024-01-10 Fate Therapeutics, Inc. Genomic engineering of pluripotent cells
CA3099441A1 (en) * 2018-06-13 2019-12-19 Regeneron Pharmaceuticals, Inc. A rodent model of fibrodysplasia ossificans progressiva
CN109504708A (zh) * 2018-12-03 2019-03-22 江苏集萃药康生物科技有限公司 一种筛选标记自我删除的基因打靶载体及方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1375654A3 (en) * 1998-11-02 2008-01-16 GTC Biotherapeutics, Inc. Transgenic and cloned mammals
US7049480B1 (en) * 2000-09-01 2006-05-23 Avigenics, Inc. Methods of enucleating an avian oocyte or zygote using two-photon laser scanning microscopy
US7803920B2 (en) * 2004-09-29 2010-09-28 Shinya Yamanaka ECAT16 gene expressed specifically in ES cells and utilization of the same
WO2009121999A1 (es) * 2008-04-04 2009-10-08 Consejo Superior De Investigaciones Científicas Promotor especifico de celulas troncales
US8518392B2 (en) * 2009-08-14 2013-08-27 Regeneron Pharmaceuticals, Inc. Promoter-regulated differentiation-dependent self-deleting cassette
CN102628061A (zh) * 2012-04-09 2012-08-08 西北农林科技大学 一种hbd3 乳腺特异性表达载体及构建的重组细胞

Also Published As

Publication number Publication date
SG11201503752SA (en) 2015-06-29
RU2015122802A (ru) 2017-01-10
IL238771A0 (en) 2015-06-30
BR112015012408A2 (pt) 2017-07-11
JP2016502407A (ja) 2016-01-28
JP2017060502A (ja) 2017-03-30
AU2013352235A1 (en) 2015-05-28
KR20150089004A (ko) 2015-08-04
ZA201503305B (en) 2016-05-25
CN104918484A (zh) 2015-09-16
US20140221734A1 (en) 2014-08-07
HK1211177A1 (en) 2016-05-20
CA2891163A1 (en) 2014-06-05
MY193790A (en) 2022-10-27
JP6227747B2 (ja) 2017-11-08
WO2014085501A1 (en) 2014-06-05
EP2925122A1 (en) 2015-10-07
MX2015006810A (es) 2015-09-07
MX353279B (es) 2018-01-05

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