RU2015117547A - Способ и системы для оптимизации системы перфузионной культуры клеток - Google Patents
Способ и системы для оптимизации системы перфузионной культуры клеток Download PDFInfo
- Publication number
- RU2015117547A RU2015117547A RU2015117547A RU2015117547A RU2015117547A RU 2015117547 A RU2015117547 A RU 2015117547A RU 2015117547 A RU2015117547 A RU 2015117547A RU 2015117547 A RU2015117547 A RU 2015117547A RU 2015117547 A RU2015117547 A RU 2015117547A
- Authority
- RU
- Russia
- Prior art keywords
- cells
- bioreactor
- initial
- perfusion
- initial volume
- Prior art date
Links
- 230000010412 perfusion Effects 0.000 title claims abstract 45
- 238000000034 method Methods 0.000 title claims 31
- 238000005457 optimization Methods 0.000 title 1
- 230000014759 maintenance of location Effects 0.000 claims abstract 21
- 239000012531 culture fluid Substances 0.000 claims abstract 17
- 230000007423 decrease Effects 0.000 claims abstract 10
- 210000004027 cell Anatomy 0.000 claims 64
- 210000004962 mammalian cell Anatomy 0.000 claims 16
- 239000004480 active ingredient Substances 0.000 claims 4
- 210000004978 chinese hamster ovary cell Anatomy 0.000 claims 4
- 101000911390 Homo sapiens Coagulation factor VIII Proteins 0.000 claims 3
- 102000057593 human F8 Human genes 0.000 claims 3
- 229960000900 human factor viii Drugs 0.000 claims 3
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims 2
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims 2
- 239000003381 stabilizer Substances 0.000 claims 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical group [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- 102000001690 Factor VIII Human genes 0.000 claims 1
- 108010054218 Factor VIII Proteins 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229960000301 factor viii Drugs 0.000 claims 1
- 230000017854 proteolysis Effects 0.000 claims 1
- 238000004064 recycling Methods 0.000 abstract 1
- 238000000926 separation method Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
- C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/02—Percolation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/10—Perfusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biophysics (AREA)
- Hematology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261712190P | 2012-10-10 | 2012-10-10 | |
| US61/712,190 | 2012-10-10 | ||
| PCT/US2013/064159 WO2014059035A1 (en) | 2012-10-10 | 2013-10-09 | Methods and systems for optimizing perfusion cell culture system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2015117547A true RU2015117547A (ru) | 2016-12-10 |
Family
ID=49448331
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2015117547A RU2015117547A (ru) | 2012-10-10 | 2013-10-09 | Способ и системы для оптимизации системы перфузионной культуры клеток |
Country Status (15)
| Country | Link |
|---|---|
| US (2) | US20150299638A1 (enExample) |
| EP (1) | EP2906677A1 (enExample) |
| JP (1) | JP6393267B2 (enExample) |
| KR (1) | KR20150063541A (enExample) |
| CN (1) | CN104822821A (enExample) |
| AR (1) | AR092967A1 (enExample) |
| AU (1) | AU2013329318A1 (enExample) |
| CA (1) | CA2887581A1 (enExample) |
| HK (1) | HK1213285A1 (enExample) |
| IL (1) | IL238179A0 (enExample) |
| MX (1) | MX2015004516A (enExample) |
| RU (1) | RU2015117547A (enExample) |
| SG (2) | SG10201705806YA (enExample) |
| TW (1) | TW201418455A (enExample) |
| WO (1) | WO2014059035A1 (enExample) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9944894B2 (en) | 2015-01-16 | 2018-04-17 | General Electric Company | Pluripotent stem cell expansion and passage using a rocking platform bioreactor |
| CN105385731B (zh) * | 2015-12-25 | 2018-10-30 | 上海莱士血液制品股份有限公司 | 一种表达重组八因子的灌注培养方法 |
| ITUB20160272A1 (it) * | 2016-01-22 | 2017-07-22 | Univ Degli Studi Di Palermo | Bioreattore a perfusione autosufficiente monouso per crescite cellulari 3D |
| CA3014584A1 (en) * | 2016-03-21 | 2017-09-28 | General Electric Company | Pluripotent stem cell expansion and passage using a stirred tank bioreactor |
| WO2019071076A1 (en) | 2017-10-06 | 2019-04-11 | Lonza Ltd. | AUTOMATED CONTROL OF CELL CULTURE BY SPECTROSCOPY RAMAN |
| HUE058924T2 (hu) | 2017-10-16 | 2022-09-28 | Regeneron Pharma | Perfúziós eljárások |
| WO2019226618A1 (en) * | 2018-05-22 | 2019-11-28 | Nantkwest, Inc. | Methods and systems for cell bed formation during bioprocessing |
| WO2019229764A1 (en) * | 2018-06-01 | 2019-12-05 | Gennova Biopharmaceuticals Limited | Process for production of recombinant tnk-tpa by packed-bed perfusion system |
| EP3875595A4 (en) * | 2018-11-02 | 2022-07-13 | Kyowa Kirin Co., Ltd. | Liquid medium preparation method |
| GB201903813D0 (en) | 2019-03-20 | 2019-05-01 | Cn Bio Innovations Ltd | Dual circulation microphysiological system |
| US12325848B2 (en) | 2019-05-15 | 2025-06-10 | Life Technologies Corporation | Cell settler apparatus systems and methods for perfusion processes |
| CN114599775B (zh) * | 2019-11-07 | 2025-07-22 | 默克专利股份公司 | 用于进行灌注细胞培养的方法和系统 |
| WO2021097281A1 (en) | 2019-11-15 | 2021-05-20 | Lonza Ltd | Process and system for producing an inoculum |
| WO2021110870A1 (en) | 2019-12-05 | 2021-06-10 | Acib Gmbh | Method for producing a fermentation product |
| EP4077629A1 (en) * | 2019-12-19 | 2022-10-26 | Universität für Bodenkultur Wien | Continuous reconstitution of process materials from solids |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6338964B1 (en) | 1999-05-07 | 2002-01-15 | Bayer Corporation | Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration |
| US20040185534A1 (en) * | 2000-10-02 | 2004-09-23 | Knudsen Ida Molgaard | Industrial-scale serum-free production of recombinant proteins in mammalian cells |
| EP1689878A4 (en) * | 2003-11-03 | 2007-02-14 | Centocor Inc | METHOD FOR MAINTAINING LOW SHEAR IN A BIOTECHNOLOGICAL SYSTEM |
| WO2007071072A1 (en) * | 2005-12-22 | 2007-06-28 | Corporation De L'ecole Polytechnique De Montreal | High-rate perfusion bioreactor |
| JP2009535019A (ja) * | 2006-04-21 | 2009-10-01 | バイエル・ヘルスケア・エルエルシー | 灌流培養への哺乳動物細胞中の抗アポトーシス遺伝子の応用 |
| WO2009023562A2 (en) * | 2007-08-09 | 2009-02-19 | Wyeth | Use of perfusion to enhance production of fed-batch cell culture in bioreactors |
| WO2009152413A1 (en) * | 2008-06-13 | 2009-12-17 | Centocor Ortho Biotech Inc. | Methods for obtaining high viable cell density in mammalian cell culture |
| EP2686415B1 (en) * | 2011-03-18 | 2017-11-15 | GE Healthcare Bio-Sciences AB | Flexible bag for cultivation of cells |
-
2013
- 2013-10-09 AU AU2013329318A patent/AU2013329318A1/en not_active Abandoned
- 2013-10-09 KR KR1020157011445A patent/KR20150063541A/ko not_active Withdrawn
- 2013-10-09 MX MX2015004516A patent/MX2015004516A/es unknown
- 2013-10-09 CN CN201380064458.7A patent/CN104822821A/zh active Pending
- 2013-10-09 HK HK16101154.2A patent/HK1213285A1/zh unknown
- 2013-10-09 EP EP13780035.5A patent/EP2906677A1/en not_active Withdrawn
- 2013-10-09 TW TW102136546A patent/TW201418455A/zh unknown
- 2013-10-09 RU RU2015117547A patent/RU2015117547A/ru not_active Application Discontinuation
- 2013-10-09 CA CA2887581A patent/CA2887581A1/en not_active Abandoned
- 2013-10-09 SG SG10201705806YA patent/SG10201705806YA/en unknown
- 2013-10-09 WO PCT/US2013/064159 patent/WO2014059035A1/en not_active Ceased
- 2013-10-09 US US14/434,913 patent/US20150299638A1/en not_active Abandoned
- 2013-10-09 US US14/049,676 patent/US20140099711A1/en not_active Abandoned
- 2013-10-09 JP JP2015536869A patent/JP6393267B2/ja not_active Expired - Fee Related
- 2013-10-09 SG SG11201502741WA patent/SG11201502741WA/en unknown
- 2013-10-10 AR ARP130103674A patent/AR092967A1/es unknown
-
2015
- 2015-04-12 IL IL238179A patent/IL238179A0/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US20140099711A1 (en) | 2014-04-10 |
| US20150299638A1 (en) | 2015-10-22 |
| WO2014059035A1 (en) | 2014-04-17 |
| EP2906677A1 (en) | 2015-08-19 |
| IL238179A0 (en) | 2015-05-31 |
| SG10201705806YA (en) | 2017-08-30 |
| JP2015531241A (ja) | 2015-11-02 |
| SG11201502741WA (en) | 2015-05-28 |
| AU2013329318A1 (en) | 2015-05-14 |
| TW201418455A (zh) | 2014-05-16 |
| HK1213285A1 (zh) | 2016-06-30 |
| MX2015004516A (es) | 2015-10-14 |
| CN104822821A (zh) | 2015-08-05 |
| JP6393267B2 (ja) | 2018-09-19 |
| AR092967A1 (es) | 2015-05-06 |
| KR20150063541A (ko) | 2015-06-09 |
| CA2887581A1 (en) | 2014-04-17 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
Effective date: 20180316 |