AU2013329318A1 - Methods and systems for optimizing perfusion cell culture system - Google Patents

Methods and systems for optimizing perfusion cell culture system Download PDF

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Publication number
AU2013329318A1
AU2013329318A1 AU2013329318A AU2013329318A AU2013329318A1 AU 2013329318 A1 AU2013329318 A1 AU 2013329318A1 AU 2013329318 A AU2013329318 A AU 2013329318A AU 2013329318 A AU2013329318 A AU 2013329318A AU 2013329318 A1 AU2013329318 A1 AU 2013329318A1
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AU
Australia
Prior art keywords
starting
bioreactor
cells
volume
perfusion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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AU2013329318A
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English (en)
Inventor
Volker Moehrle
Yuval SHIMONI
Venkatesh Srinivasan
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Healthcare LLC
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Bayer Healthcare LLC
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Filing date
Publication date
Application filed by Bayer Healthcare LLC filed Critical Bayer Healthcare LLC
Publication of AU2013329318A1 publication Critical patent/AU2013329318A1/en
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/14—Bioreactors or fermenters specially adapted for specific uses for producing enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/02—Percolation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/44—Means for regulation, monitoring, measurement or control, e.g. flow regulation of volume or liquid level
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/10—Separation or concentration of fermentation products

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Hematology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AU2013329318A 2012-10-10 2013-10-09 Methods and systems for optimizing perfusion cell culture system Abandoned AU2013329318A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261712190P 2012-10-10 2012-10-10
US61/712,190 2012-10-10
PCT/US2013/064159 WO2014059035A1 (en) 2012-10-10 2013-10-09 Methods and systems for optimizing perfusion cell culture system

Publications (1)

Publication Number Publication Date
AU2013329318A1 true AU2013329318A1 (en) 2015-05-14

Family

ID=49448331

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2013329318A Abandoned AU2013329318A1 (en) 2012-10-10 2013-10-09 Methods and systems for optimizing perfusion cell culture system

Country Status (15)

Country Link
US (2) US20150299638A1 (enExample)
EP (1) EP2906677A1 (enExample)
JP (1) JP6393267B2 (enExample)
KR (1) KR20150063541A (enExample)
CN (1) CN104822821A (enExample)
AR (1) AR092967A1 (enExample)
AU (1) AU2013329318A1 (enExample)
CA (1) CA2887581A1 (enExample)
HK (1) HK1213285A1 (enExample)
IL (1) IL238179A0 (enExample)
MX (1) MX2015004516A (enExample)
RU (1) RU2015117547A (enExample)
SG (2) SG10201705806YA (enExample)
TW (1) TW201418455A (enExample)
WO (1) WO2014059035A1 (enExample)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9944894B2 (en) 2015-01-16 2018-04-17 General Electric Company Pluripotent stem cell expansion and passage using a rocking platform bioreactor
CN105385731B (zh) * 2015-12-25 2018-10-30 上海莱士血液制品股份有限公司 一种表达重组八因子的灌注培养方法
ITUB20160272A1 (it) * 2016-01-22 2017-07-22 Univ Degli Studi Di Palermo Bioreattore a perfusione autosufficiente monouso per crescite cellulari 3D
CA3014584A1 (en) * 2016-03-21 2017-09-28 General Electric Company Pluripotent stem cell expansion and passage using a stirred tank bioreactor
WO2019071076A1 (en) 2017-10-06 2019-04-11 Lonza Ltd. AUTOMATED CONTROL OF CELL CULTURE BY SPECTROSCOPY RAMAN
HUE058924T2 (hu) 2017-10-16 2022-09-28 Regeneron Pharma Perfúziós eljárások
WO2019226618A1 (en) * 2018-05-22 2019-11-28 Nantkwest, Inc. Methods and systems for cell bed formation during bioprocessing
WO2019229764A1 (en) * 2018-06-01 2019-12-05 Gennova Biopharmaceuticals Limited Process for production of recombinant tnk-tpa by packed-bed perfusion system
EP3875595A4 (en) * 2018-11-02 2022-07-13 Kyowa Kirin Co., Ltd. Liquid medium preparation method
GB201903813D0 (en) 2019-03-20 2019-05-01 Cn Bio Innovations Ltd Dual circulation microphysiological system
US12325848B2 (en) 2019-05-15 2025-06-10 Life Technologies Corporation Cell settler apparatus systems and methods for perfusion processes
CN114599775B (zh) * 2019-11-07 2025-07-22 默克专利股份公司 用于进行灌注细胞培养的方法和系统
WO2021097281A1 (en) 2019-11-15 2021-05-20 Lonza Ltd Process and system for producing an inoculum
WO2021110870A1 (en) 2019-12-05 2021-06-10 Acib Gmbh Method for producing a fermentation product
EP4077629A1 (en) * 2019-12-19 2022-10-26 Universität für Bodenkultur Wien Continuous reconstitution of process materials from solids

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6338964B1 (en) 1999-05-07 2002-01-15 Bayer Corporation Process and medium for mammalian cell culture under low dissolved carbon dioxide concentration
US20040185534A1 (en) * 2000-10-02 2004-09-23 Knudsen Ida Molgaard Industrial-scale serum-free production of recombinant proteins in mammalian cells
EP1689878A4 (en) * 2003-11-03 2007-02-14 Centocor Inc METHOD FOR MAINTAINING LOW SHEAR IN A BIOTECHNOLOGICAL SYSTEM
WO2007071072A1 (en) * 2005-12-22 2007-06-28 Corporation De L'ecole Polytechnique De Montreal High-rate perfusion bioreactor
JP2009535019A (ja) * 2006-04-21 2009-10-01 バイエル・ヘルスケア・エルエルシー 灌流培養への哺乳動物細胞中の抗アポトーシス遺伝子の応用
WO2009023562A2 (en) * 2007-08-09 2009-02-19 Wyeth Use of perfusion to enhance production of fed-batch cell culture in bioreactors
WO2009152413A1 (en) * 2008-06-13 2009-12-17 Centocor Ortho Biotech Inc. Methods for obtaining high viable cell density in mammalian cell culture
EP2686415B1 (en) * 2011-03-18 2017-11-15 GE Healthcare Bio-Sciences AB Flexible bag for cultivation of cells

Also Published As

Publication number Publication date
US20140099711A1 (en) 2014-04-10
US20150299638A1 (en) 2015-10-22
WO2014059035A1 (en) 2014-04-17
EP2906677A1 (en) 2015-08-19
IL238179A0 (en) 2015-05-31
SG10201705806YA (en) 2017-08-30
JP2015531241A (ja) 2015-11-02
SG11201502741WA (en) 2015-05-28
TW201418455A (zh) 2014-05-16
HK1213285A1 (zh) 2016-06-30
MX2015004516A (es) 2015-10-14
CN104822821A (zh) 2015-08-05
JP6393267B2 (ja) 2018-09-19
RU2015117547A (ru) 2016-12-10
AR092967A1 (es) 2015-05-06
KR20150063541A (ko) 2015-06-09
CA2887581A1 (en) 2014-04-17

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