RU2012133474A - Очистка факторов свертывания крови - Google Patents
Очистка факторов свертывания крови Download PDFInfo
- Publication number
- RU2012133474A RU2012133474A RU2012133474/10A RU2012133474A RU2012133474A RU 2012133474 A RU2012133474 A RU 2012133474A RU 2012133474/10 A RU2012133474/10 A RU 2012133474/10A RU 2012133474 A RU2012133474 A RU 2012133474A RU 2012133474 A RU2012133474 A RU 2012133474A
- Authority
- RU
- Russia
- Prior art keywords
- gla
- protein
- polypeptide
- gamma
- carboxyglutamic acid
- Prior art date
Links
- 239000008280 blood Substances 0.000 title 1
- 210000004369 blood Anatomy 0.000 title 1
- 238000004140 cleaning Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 27
- UHBYWPGGCSDKFX-VKHMYHEASA-N gamma-carboxy-L-glutamic acid Chemical compound OC(=O)[C@@H](N)CC(C(O)=O)C(O)=O UHBYWPGGCSDKFX-VKHMYHEASA-N 0.000 claims abstract 14
- 229920001184 polypeptide Polymers 0.000 claims abstract 14
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract 14
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract 14
- 102000004169 proteins and genes Human genes 0.000 claims abstract 13
- 108090000623 proteins and genes Proteins 0.000 claims abstract 13
- 239000000463 material Substances 0.000 claims abstract 9
- 102100022641 Coagulation factor IX Human genes 0.000 claims abstract 8
- 108010076282 Factor IX Proteins 0.000 claims abstract 8
- 229960004222 factor ix Drugs 0.000 claims abstract 8
- 239000000203 mixture Substances 0.000 claims abstract 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims 4
- 239000001110 calcium chloride Substances 0.000 claims 4
- 229910001628 calcium chloride Inorganic materials 0.000 claims 4
- BHPQYMZQTOCNFJ-UHFFFAOYSA-N Calcium cation Chemical compound [Ca+2] BHPQYMZQTOCNFJ-UHFFFAOYSA-N 0.000 claims 3
- 229920002684 Sepharose Polymers 0.000 claims 3
- 229910001424 calcium ion Inorganic materials 0.000 claims 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims 2
- 239000011324 bead Substances 0.000 claims 2
- 239000012149 elution buffer Substances 0.000 claims 2
- -1 3.0 Chemical compound 0.000 claims 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 claims 1
- 238000005571 anion exchange chromatography Methods 0.000 claims 1
- 239000000872 buffer Substances 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 150000001768 cations Chemical class 0.000 claims 1
- 239000006167 equilibration buffer Substances 0.000 claims 1
- 125000005647 linker group Chemical group 0.000 claims 1
- 239000011777 magnesium Substances 0.000 claims 1
- 229910052749 magnesium Inorganic materials 0.000 claims 1
- 238000000746 purification Methods 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000011534 wash buffer Substances 0.000 claims 1
- 102000010410 Nogo Proteins Human genes 0.000 abstract 1
- 108010077641 Nogo Proteins Proteins 0.000 abstract 1
- 238000010828 elution Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/644—Coagulation factor IXa (3.4.21.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21022—Coagulation factor IXa (3.4.21.22)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10150980 | 2010-01-18 | ||
| EP10150980.0 | 2010-01-18 | ||
| US29701110P | 2010-01-21 | 2010-01-21 | |
| US61/297,011 | 2010-01-21 | ||
| PCT/EP2011/050594 WO2011086197A1 (en) | 2010-01-18 | 2011-01-18 | Purification of blood coagulation factors |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2012133474A true RU2012133474A (ru) | 2014-02-27 |
Family
ID=42173365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2012133474/10A RU2012133474A (ru) | 2010-01-18 | 2011-01-18 | Очистка факторов свертывания крови |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9896677B2 (enExample) |
| EP (1) | EP2526115B1 (enExample) |
| JP (1) | JP6250931B2 (enExample) |
| KR (1) | KR20120118028A (enExample) |
| CN (1) | CN102939299B (enExample) |
| BR (1) | BR112012017696A2 (enExample) |
| RU (1) | RU2012133474A (enExample) |
| WO (1) | WO2011086197A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2526115B1 (en) * | 2010-01-18 | 2018-08-01 | Novo Nordisk Health Care AG | Purification of blood coagulation factors |
| CN103539852B (zh) * | 2012-07-12 | 2015-08-12 | 上海泰龙生物医药科技有限公司 | 一种从细胞培养液中分离纯化重组人凝血八因子的方法 |
| CN107880126B (zh) | 2012-12-21 | 2021-03-30 | 西雅图基因公司 | 抗ntb-a抗体及相关组合物和方法 |
| US10643423B2 (en) | 2016-09-23 | 2020-05-05 | Sg Gaming, Inc. | System and digital table for binding a mobile device to a position at the table for transactions |
Family Cites Families (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6258938B1 (en) | 1983-10-28 | 2001-07-10 | Ne Medical Center Hospital, Inc. | Method for the purification and isolation of blood clotting proteins using conformation specific antibodies |
| US4902614A (en) | 1984-12-03 | 1990-02-20 | Teijin Limited | Monoclonal antibody to human protein C |
| FR2632309B1 (fr) | 1988-06-07 | 1990-08-24 | Lille Transfusion Sanguine | Procede de purification par voie chromatographique de proteines, notamment de facteur viii, et les produits obtenus |
| US4981952A (en) | 1988-10-04 | 1991-01-01 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
| EP0573605B1 (en) | 1991-03-01 | 2000-08-30 | Rhone-Poulenc Rorer International (Holdings) Inc. | Preparation of factor ix |
| IT1262899B (it) | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| DE4406515C1 (de) | 1994-02-28 | 1995-10-19 | Immuno Ag | Verfahren zur Isolierung und Reinigung Vitamin K-abhängiger Proteine |
| US6451987B1 (en) | 1999-03-15 | 2002-09-17 | Novo Nordisk A/S | Ion exchange chromatography of proteins and peptides |
| SE0000675D0 (sv) | 2000-03-02 | 2000-03-02 | Protease Ab | Monoclonal antibodies |
| KR101204712B1 (ko) | 2003-03-18 | 2012-11-27 | 노보 노르디스크 헬스 케어 악티엔게젤샤프트 | Ｇｌａ-잔기 함유 세린 프로테아제의 제조 방법 |
| JP2008525381A (ja) | 2004-12-23 | 2008-07-17 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | 関心のあるビタミンｋ依存性タンパク質を含んでなる組成物中におけるタンパク質混入物の量の減少 |
| DE602006017882D1 (de) | 2005-12-02 | 2010-12-09 | Univ Wake Forest Health Sciences | Zusammensetzungen und verfahren zur erhöhung der produktion rekombinanter gamma-carboxylierter proteine |
| EP2125866B1 (en) | 2007-02-28 | 2013-05-22 | Baxter International Inc. | Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules |
| AU2009324204B2 (en) | 2008-12-02 | 2014-10-16 | Novo Nordisk Health Care Ag | Polypeptide purification |
| EP2526115B1 (en) * | 2010-01-18 | 2018-08-01 | Novo Nordisk Health Care AG | Purification of blood coagulation factors |
-
2011
- 2011-01-18 EP EP11700547.0A patent/EP2526115B1/en not_active Not-in-force
- 2011-01-18 BR BR112012017696A patent/BR112012017696A2/pt not_active IP Right Cessation
- 2011-01-18 RU RU2012133474/10A patent/RU2012133474A/ru unknown
- 2011-01-18 KR KR1020127020380A patent/KR20120118028A/ko not_active Withdrawn
- 2011-01-18 JP JP2012548460A patent/JP6250931B2/ja not_active Expired - Fee Related
- 2011-01-18 US US13/522,548 patent/US9896677B2/en active Active
- 2011-01-18 WO PCT/EP2011/050594 patent/WO2011086197A1/en not_active Ceased
- 2011-01-18 CN CN201180006396.5A patent/CN102939299B/zh not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN102939299A (zh) | 2013-02-20 |
| CN102939299B (zh) | 2016-04-20 |
| JP6250931B2 (ja) | 2017-12-20 |
| EP2526115B1 (en) | 2018-08-01 |
| US9896677B2 (en) | 2018-02-20 |
| US20130034896A1 (en) | 2013-02-07 |
| WO2011086197A1 (en) | 2011-07-21 |
| JP2013517259A (ja) | 2013-05-16 |
| EP2526115A1 (en) | 2012-11-28 |
| BR112012017696A2 (pt) | 2017-10-03 |
| KR20120118028A (ko) | 2012-10-25 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| HE9A | Changing address for correspondence with an applicant | ||
| FA91 | Application withdrawn (on applicant's request) |
Effective date: 20150615 |