CN102939299B - 凝血因子的纯化 - Google Patents
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- 235000017281 sodium acetate Nutrition 0.000 description 1
- 235000017550 sodium carbonate Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 229910001427 strontium ion Inorganic materials 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 150000003573 thiols Chemical class 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000001052 transient effect Effects 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/647—Blood coagulation factors not provided for in a preceding group or according to more than one of the proceeding groups
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/745—Blood coagulation or fibrinolysis factors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/644—Coagulation factor IXa (3.4.21.22)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21022—Coagulation factor IXa (3.4.21.22)
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biomedical Technology (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP10150980 | 2010-01-18 | ||
| EP10150980.0 | 2010-01-18 | ||
| US29701110P | 2010-01-21 | 2010-01-21 | |
| US61/297011 | 2010-01-21 | ||
| PCT/EP2011/050594 WO2011086197A1 (en) | 2010-01-18 | 2011-01-18 | Purification of blood coagulation factors |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102939299A CN102939299A (zh) | 2013-02-20 |
| CN102939299B true CN102939299B (zh) | 2016-04-20 |
Family
ID=42173365
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201180006396.5A Expired - Fee Related CN102939299B (zh) | 2010-01-18 | 2011-01-18 | 凝血因子的纯化 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US9896677B2 (enExample) |
| EP (1) | EP2526115B1 (enExample) |
| JP (1) | JP6250931B2 (enExample) |
| KR (1) | KR20120118028A (enExample) |
| CN (1) | CN102939299B (enExample) |
| BR (1) | BR112012017696A2 (enExample) |
| RU (1) | RU2012133474A (enExample) |
| WO (1) | WO2011086197A1 (enExample) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2526115B1 (en) * | 2010-01-18 | 2018-08-01 | Novo Nordisk Health Care AG | Purification of blood coagulation factors |
| CN103539852B (zh) * | 2012-07-12 | 2015-08-12 | 上海泰龙生物医药科技有限公司 | 一种从细胞培养液中分离纯化重组人凝血八因子的方法 |
| CN107880126B (zh) | 2012-12-21 | 2021-03-30 | 西雅图基因公司 | 抗ntb-a抗体及相关组合物和方法 |
| US10643423B2 (en) | 2016-09-23 | 2020-05-05 | Sg Gaming, Inc. | System and digital table for binding a mobile device to a position at the table for transactions |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5633350A (en) * | 1994-02-28 | 1997-05-27 | Immuno Aktiengesellschaft | Method for the isolation and purification of vitamin K-dependent proteins |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6258938B1 (en) | 1983-10-28 | 2001-07-10 | Ne Medical Center Hospital, Inc. | Method for the purification and isolation of blood clotting proteins using conformation specific antibodies |
| US4902614A (en) | 1984-12-03 | 1990-02-20 | Teijin Limited | Monoclonal antibody to human protein C |
| FR2632309B1 (fr) | 1988-06-07 | 1990-08-24 | Lille Transfusion Sanguine | Procede de purification par voie chromatographique de proteines, notamment de facteur viii, et les produits obtenus |
| US4981952A (en) | 1988-10-04 | 1991-01-01 | Eli Lilly And Company | Method for the purification of vitamin K-dependent proteins |
| EP0573605B1 (en) | 1991-03-01 | 2000-08-30 | Rhone-Poulenc Rorer International (Holdings) Inc. | Preparation of factor ix |
| IT1262899B (it) | 1992-03-27 | 1996-07-22 | Sclavo Spa | Processo per l'isolamento di fattore ix, fattore x e fattore ii altamente purificati dal complesso protrombinico o dal plasma umano |
| US6451987B1 (en) | 1999-03-15 | 2002-09-17 | Novo Nordisk A/S | Ion exchange chromatography of proteins and peptides |
| SE0000675D0 (sv) | 2000-03-02 | 2000-03-02 | Protease Ab | Monoclonal antibodies |
| KR101204712B1 (ko) | 2003-03-18 | 2012-11-27 | 노보 노르디스크 헬스 케어 악티엔게젤샤프트 | Ｇｌａ-잔기 함유 세린 프로테아제의 제조 방법 |
| JP2008525381A (ja) | 2004-12-23 | 2008-07-17 | ノボ ノルディスク ヘルス ケア アクチェンゲゼルシャフト | 関心のあるビタミンｋ依存性タンパク質を含んでなる組成物中におけるタンパク質混入物の量の減少 |
| DE602006017882D1 (de) | 2005-12-02 | 2010-12-09 | Univ Wake Forest Health Sciences | Zusammensetzungen und verfahren zur erhöhung der produktion rekombinanter gamma-carboxylierter proteine |
| EP2125866B1 (en) | 2007-02-28 | 2013-05-22 | Baxter International Inc. | Method for the purification of recombinant blood coagulation factor ix enriched in sulfated and/or phosphorylated molecules |
| AU2009324204B2 (en) | 2008-12-02 | 2014-10-16 | Novo Nordisk Health Care Ag | Polypeptide purification |
| EP2526115B1 (en) * | 2010-01-18 | 2018-08-01 | Novo Nordisk Health Care AG | Purification of blood coagulation factors |
-
2011
- 2011-01-18 EP EP11700547.0A patent/EP2526115B1/en not_active Not-in-force
- 2011-01-18 BR BR112012017696A patent/BR112012017696A2/pt not_active IP Right Cessation
- 2011-01-18 RU RU2012133474/10A patent/RU2012133474A/ru unknown
- 2011-01-18 KR KR1020127020380A patent/KR20120118028A/ko not_active Withdrawn
- 2011-01-18 JP JP2012548460A patent/JP6250931B2/ja not_active Expired - Fee Related
- 2011-01-18 US US13/522,548 patent/US9896677B2/en active Active
- 2011-01-18 WO PCT/EP2011/050594 patent/WO2011086197A1/en not_active Ceased
- 2011-01-18 CN CN201180006396.5A patent/CN102939299B/zh not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5633350A (en) * | 1994-02-28 | 1997-05-27 | Immuno Aktiengesellschaft | Method for the isolation and purification of vitamin K-dependent proteins |
Non-Patent Citations (2)
| Title |
|---|
| Affinity purification of biologically active and inactive forms of recombinant human protein C produced in porcine mammary gland;Kevin E. Van Cott et al.;《Journal of molecular recognition》;19961231;第9卷(第5-6期);摘要，第408页左栏第1段，第409页左栏第3段、右栏第2段，第410页右栏第1段，第412页右栏第3段，第413页左栏第4段，图1-图5及图5注解 * |
| Increased production of functional recombinant human clotting factor IX by baby hamster kidney cells engineered to overexpress VKORC1，the Viatmin K 2,3-expoxide-reducing enzyme of the vitamin K cycle;Nadeem Wajih et al.;《The journal of biological chemistry》;20050909;第280卷(第36期);第31603页左栏第2段至右栏第1段，第31604页右栏第1段，第31605页右栏第1段，第31606页左栏第1段，第31607页左栏第2段，图1及图1注解 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102939299A (zh) | 2013-02-20 |
| JP6250931B2 (ja) | 2017-12-20 |
| EP2526115B1 (en) | 2018-08-01 |
| US9896677B2 (en) | 2018-02-20 |
| US20130034896A1 (en) | 2013-02-07 |
| WO2011086197A1 (en) | 2011-07-21 |
| JP2013517259A (ja) | 2013-05-16 |
| EP2526115A1 (en) | 2012-11-28 |
| RU2012133474A (ru) | 2014-02-27 |
| BR112012017696A2 (pt) | 2017-10-03 |
| KR20120118028A (ko) | 2012-10-25 |
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Granted publication date: 20160420 Termination date: 20190118 |