RU2011107993A - Алгоритм обнаружения для пцр-анализа - Google Patents
Алгоритм обнаружения для пцр-анализа Download PDFInfo
- Publication number
- RU2011107993A RU2011107993A RU2011107993/10A RU2011107993A RU2011107993A RU 2011107993 A RU2011107993 A RU 2011107993A RU 2011107993/10 A RU2011107993/10 A RU 2011107993/10A RU 2011107993 A RU2011107993 A RU 2011107993A RU 2011107993 A RU2011107993 A RU 2011107993A
- Authority
- RU
- Russia
- Prior art keywords
- approximately
- specified
- target nucleic
- nucleic acid
- nucleotide probe
- Prior art date
Links
- 238000001514 detection method Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract 36
- 239000000523 sample Substances 0.000 claims abstract 31
- 150000007523 nucleic acids Chemical class 0.000 claims abstract 26
- 102000039446 nucleic acids Human genes 0.000 claims abstract 26
- 108020004707 nucleic acids Proteins 0.000 claims abstract 26
- 239000002773 nucleotide Substances 0.000 claims abstract 22
- 125000003729 nucleotide group Chemical group 0.000 claims abstract 22
- 238000009396 hybridization Methods 0.000 claims abstract 5
- 238000002844 melting Methods 0.000 claims abstract 4
- 230000008018 melting Effects 0.000 claims abstract 4
- 238000004020 luminiscence type Methods 0.000 claims abstract 3
- 238000005259 measurement Methods 0.000 claims 6
- 239000007850 fluorescent dye Substances 0.000 claims 5
- 230000003993 interaction Effects 0.000 claims 4
- 239000011541 reaction mixture Substances 0.000 claims 4
- 102000004190 Enzymes Human genes 0.000 claims 2
- 108090000790 Enzymes Proteins 0.000 claims 2
- 230000003321 amplification Effects 0.000 claims 2
- 238000000137 annealing Methods 0.000 claims 2
- 125000004122 cyclic group Chemical group 0.000 claims 2
- 238000004925 denaturation Methods 0.000 claims 2
- 230000036425 denaturation Effects 0.000 claims 2
- 238000003199 nucleic acid amplification method Methods 0.000 claims 2
- 230000005855 radiation Effects 0.000 claims 2
- 239000000758 substrate Substances 0.000 claims 2
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2527/00—Reactions demanding special reaction conditions
- C12Q2527/107—Temperature of melting, i.e. Tm
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US13604008P | 2008-08-08 | 2008-08-08 | |
| US61/136,040 | 2008-08-08 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2011107993A true RU2011107993A (ru) | 2012-09-20 |
Family
ID=41259286
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2011107993/10A RU2011107993A (ru) | 2008-08-08 | 2009-08-10 | Алгоритм обнаружения для пцр-анализа |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US20110151461A1 (enExample) |
| EP (1) | EP2315844A1 (enExample) |
| JP (1) | JP2011530296A (enExample) |
| KR (1) | KR20110052681A (enExample) |
| CN (1) | CN102112632A (enExample) |
| AU (1) | AU2009279457A1 (enExample) |
| BR (1) | BRPI0917434A2 (enExample) |
| CA (1) | CA2733186A1 (enExample) |
| RU (1) | RU2011107993A (enExample) |
| WO (1) | WO2010017543A1 (enExample) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010068576A1 (en) * | 2008-12-10 | 2010-06-17 | Smiths Detection Inc. | Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization |
| US20130203057A1 (en) * | 2012-01-20 | 2013-08-08 | Biohelix Corporation | Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions |
| CN104271767B (zh) | 2012-02-03 | 2017-08-25 | 加州理工学院 | 多路生化测定中信号的编码和解码 |
| IN2015DN01609A (enExample) | 2012-08-03 | 2015-07-03 | California Inst Of Techn | |
| WO2015147370A1 (en) | 2014-03-28 | 2015-10-01 | Seegene, Inc. | Detection of target nucleic acid sequences using different detection temperatures |
| US10876155B2 (en) | 2014-12-09 | 2020-12-29 | Seegene, Inc. | Differentiation of signals for target nucleic acid sequences |
| JP2018504096A (ja) * | 2014-12-09 | 2018-02-15 | シージーン アイエヌシー | 異なる検出温度及び基準値を使用したターゲット核酸配列の検出 |
| KR102110999B1 (ko) * | 2016-01-26 | 2020-05-14 | 주식회사 씨젠 | 타겟 핵산 서열에 대한 시그널을 제공하는 방법 |
| JP2021511056A (ja) * | 2018-01-22 | 2021-05-06 | ルミネックス コーポレーション | 不連続融解分析のための方法および組成物 |
| WO2019204357A1 (en) | 2018-04-17 | 2019-10-24 | ChromaCode, Inc. | Methods and systems for multiplex analysis |
| US12203129B2 (en) | 2018-07-03 | 2025-01-21 | ChromaCode, Inc. | Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays |
| EP4421814A4 (en) * | 2021-10-21 | 2025-11-12 | Seegene Inc | APPROVAL AND METHOD FOR READING A POSITIVE/NEGATIVE RESULT WITH RESPECT TO A TARGET TEST SUBSTANCE IN A SAMPLE |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4683202A (en) * | 1985-03-28 | 1987-07-28 | Cetus Corporation | Process for amplifying nucleic acid sequences |
| US5475098A (en) * | 1994-06-14 | 1995-12-12 | The United States Of America As Represented By The Department Of Health And Human Services | Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli |
| JP4646404B2 (ja) * | 1998-07-02 | 2011-03-09 | ジェン−プローブ・インコーポレーテッド | 分子トーチ（ｔｏｒｃｈ） |
| US20050202470A1 (en) * | 2000-11-16 | 2005-09-15 | Caliper Life Sciences, Inc. | Binding assays using molecular melt curves |
| US8900811B2 (en) * | 2000-11-16 | 2014-12-02 | Caliper Life Sciences, Inc. | Method and apparatus for generating thermal melting curves in a microfluidic device |
| WO2003037514A2 (en) * | 2001-10-30 | 2003-05-08 | The Texas A & M University System | Method and apparatus for temperature gradient microfluidics |
| DE602006021056D1 (de) * | 2005-11-14 | 2011-05-12 | Gen Probe Inc | Parametrisches kalibrationsverfahren |
-
2009
- 2009-08-10 EP EP09791325A patent/EP2315844A1/en not_active Withdrawn
- 2009-08-10 WO PCT/US2009/053253 patent/WO2010017543A1/en not_active Ceased
- 2009-08-10 US US13/057,735 patent/US20110151461A1/en not_active Abandoned
- 2009-08-10 RU RU2011107993/10A patent/RU2011107993A/ru not_active Application Discontinuation
- 2009-08-10 AU AU2009279457A patent/AU2009279457A1/en not_active Abandoned
- 2009-08-10 JP JP2011522302A patent/JP2011530296A/ja active Pending
- 2009-08-10 CA CA2733186A patent/CA2733186A1/en not_active Abandoned
- 2009-08-10 KR KR1020117005036A patent/KR20110052681A/ko not_active Withdrawn
- 2009-08-10 BR BRPI0917434A patent/BRPI0917434A2/pt not_active Application Discontinuation
- 2009-08-10 CN CN2009801308017A patent/CN102112632A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| WO2010017543A1 (en) | 2010-02-11 |
| EP2315844A1 (en) | 2011-05-04 |
| CN102112632A (zh) | 2011-06-29 |
| AU2009279457A1 (en) | 2010-02-11 |
| KR20110052681A (ko) | 2011-05-18 |
| BRPI0917434A2 (pt) | 2015-12-01 |
| JP2011530296A (ja) | 2011-12-22 |
| US20110151461A1 (en) | 2011-06-23 |
| CA2733186A1 (en) | 2010-02-11 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2011107993A (ru) | Алгоритм обнаружения для пцр-анализа | |
| JP2011530296A5 (enExample) | ||
| EP1716257B8 (en) | New primers and probes for the detection of parvovirus b19 | |
| JP6085564B2 (ja) | 標的核酸の検出法 | |
| US7081339B2 (en) | Methods for variation detection | |
| HUP0101385A2 (hu) | Eljárás nukleinsavak detektálására PCR-módszerrel | |
| RU2011117297A (ru) | Праймеры и зонды для обнаружения вируса папилломы человека и последовательностей бета-глобина человека в исследуемых образцах | |
| JP2008516612A5 (enExample) | ||
| US20130065237A1 (en) | Novel mixtures for assaying nucleic acid, novel method of assaying nucleic acid with the use of the same and nucleic acid probe to be used therefor | |
| CA2784344A1 (en) | Tsg primer target detection | |
| KR20120042100A (ko) | 이중표지 고정화 프로브를 이용한 고상에서의 타겟 핵산서열 검출 | |
| CN115948605A (zh) | 一种基于dna链置换反应与per扩增的病毒检测试剂盒 | |
| Cheng et al. | Ligase chain reaction coupled with rolling circle amplification for high sensitivity detection of single nucleotide polymorphisms | |
| RU2558236C2 (ru) | Система анализа для обнаружения близкородственных серотипов вируса папилломы человека (впч) | |
| KR101190792B1 (ko) | 변형 뉴클레오티드 및 이를 이용한 실시간 중합효소 반응 | |
| RU2011132045A (ru) | Праймеры и зонды для детектирования вируса гепатита с | |
| WO2011025329A2 (en) | Detecting methods of dna amplification using new intercalating agent | |
| US20110015086A1 (en) | Programmable oligonucleotme micro array | |
| CA2549059A1 (en) | Oligonucleotides for the detection of hepatitis b virus | |
| JP6720161B2 (ja) | 複数の標的核酸の検出キット及びそれを用いる検出方法 | |
| CN110546273A (zh) | 通过衔接子序列定量ngs dna | |
| EP3156501A1 (en) | Method for detecting escherichia coli and carrier for detecting escherichia coli | |
| US20180087096A1 (en) | Gene mutation detection method and fluorescence-labeled oligonucleotide used in same | |
| JP2004313120A (ja) | β３アドレナリン受容体変異遺伝子の検出法ならびにそのための核酸プローブおよびキット | |
| EP2242858A2 (en) | Isothermal detection methods and uses thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA93 | Acknowledgement of application withdrawn (no request for examination) |
Effective date: 20120813 |