JP2011530296A - Ｐｃｒアッセイのための検出アルゴリズム - Google Patents

Ｐｃｒアッセイのための検出アルゴリズム Download PDF

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Publication number
JP2011530296A
JP2011530296A JP2011522302A JP2011522302A JP2011530296A JP 2011530296 A JP2011530296 A JP 2011530296A JP 2011522302 A JP2011522302 A JP 2011522302A JP 2011522302 A JP2011522302 A JP 2011522302A JP 2011530296 A JP2011530296 A JP 2011530296A
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JP
Japan
Prior art keywords
nucleic acid
temperature
target nucleic
probe
label
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Pending
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JP2011522302A
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English (en)
Japanese (ja)
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JP2011530296A5 (enExample
Inventor
ジョン ロバート リンク
バリー エドワード ボエス
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Smiths Detection Inc
Original Assignee
Smiths Detection Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Smiths Detection Inc filed Critical Smiths Detection Inc
Publication of JP2011530296A publication Critical patent/JP2011530296A/ja
Publication of JP2011530296A5 publication Critical patent/JP2011530296A5/ja
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
JP2011522302A 2008-08-08 2009-08-10 Ｐｃｒアッセイのための検出アルゴリズム Pending JP2011530296A (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US13604008P 2008-08-08 2008-08-08
US61/136,040 2008-08-08
PCT/US2009/053253 WO2010017543A1 (en) 2008-08-08 2009-08-10 Detection algorithm for pcr assay

Publications (2)

Publication Number Publication Date
JP2011530296A true JP2011530296A (ja) 2011-12-22
JP2011530296A5 JP2011530296A5 (enExample) 2012-02-09

Family

ID=41259286

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011522302A Pending JP2011530296A (ja) 2008-08-08 2009-08-10 Ｐｃｒアッセイのための検出アルゴリズム

Country Status (10)

Country Link
US (1) US20110151461A1 (enExample)
EP (1) EP2315844A1 (enExample)
JP (1) JP2011530296A (enExample)
KR (1) KR20110052681A (enExample)
CN (1) CN102112632A (enExample)
AU (1) AU2009279457A1 (enExample)
BR (1) BRPI0917434A2 (enExample)
CA (1) CA2733186A1 (enExample)
RU (1) RU2011107993A (enExample)
WO (1) WO2010017543A1 (enExample)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017518028A (ja) * 2014-03-28 2017-07-06 シージーン アイエヌシー 相異なる検出温度を利用したターゲット核酸配列の検出
JP2021511056A (ja) * 2018-01-22 2021-05-06 ルミネックス コーポレーション 不連続融解分析のための方法および組成物

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010068576A1 (en) * 2008-12-10 2010-06-17 Smiths Detection Inc. Identification and differentiation of nucleic acid sequence using temperature-dependent hybridization
US20130203057A1 (en) * 2012-01-20 2013-08-08 Biohelix Corporation Step-wise detection of multiple target sequences in isothermal nucleic acid amplification reactions
CN104271767B (zh) 2012-02-03 2017-08-25 加州理工学院 多路生化测定中信号的编码和解码
IN2015DN01609A (enExample) 2012-08-03 2015-07-03 California Inst Of Techn
US10876155B2 (en) 2014-12-09 2020-12-29 Seegene, Inc. Differentiation of signals for target nucleic acid sequences
JP2018504096A (ja) * 2014-12-09 2018-02-15 シージーン アイエヌシー 異なる検出温度及び基準値を使用したターゲット核酸配列の検出
KR102110999B1 (ko) * 2016-01-26 2020-05-14 주식회사 씨젠 타겟 핵산 서열에 대한 시그널을 제공하는 방법
WO2019204357A1 (en) 2018-04-17 2019-10-24 ChromaCode, Inc. Methods and systems for multiplex analysis
US12203129B2 (en) 2018-07-03 2025-01-21 ChromaCode, Inc. Formulations and signal encoding and decoding methods for massively multiplexed biochemical assays
EP4421814A4 (en) * 2021-10-21 2025-11-12 Seegene Inc APPROVAL AND METHOD FOR READING A POSITIVE/NEGATIVE RESULT WITH RESPECT TO A TARGET TEST SUBSTANCE IN A SAMPLE

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5475098A (en) * 1994-06-14 1995-12-12 The United States Of America As Represented By The Department Of Health And Human Services Distinctive DNA sequence of E. coli 0157:H7 and its use for the rapid, sensitive and specific detection of 0157:H7 and other enterohemorrhagic E. coli
JP4646404B2 (ja) * 1998-07-02 2011-03-09 ジェン−プローブ・インコーポレーテッド 分子トーチ（ｔｏｒｃｈ）
US20050202470A1 (en) * 2000-11-16 2005-09-15 Caliper Life Sciences, Inc. Binding assays using molecular melt curves
US8900811B2 (en) * 2000-11-16 2014-12-02 Caliper Life Sciences, Inc. Method and apparatus for generating thermal melting curves in a microfluidic device
WO2003037514A2 (en) * 2001-10-30 2003-05-08 The Texas A & M University System Method and apparatus for temperature gradient microfluidics
DE602006021056D1 (de) * 2005-11-14 2011-05-12 Gen Probe Inc Parametrisches kalibrationsverfahren

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
JPN6013035615; Ｎｕｃｌｅｉｃ　Ａｃｉｄｓ　Ｒｅｓｅａｒｃｈ Ｖｏｌ．３１，　Ｎｏ．４, 2003, ｐ．１３１９-１３３０ *
JPN6013035617; Ｃｌｉｎｉｃａ　Ｃｈｉｍｉｃａ　Ａｃｔａ Ｖｏｌ．３６３, 2006, ｐ．４８-６０ *

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2017518028A (ja) * 2014-03-28 2017-07-06 シージーン アイエヌシー 相異なる検出温度を利用したターゲット核酸配列の検出
JP2021511056A (ja) * 2018-01-22 2021-05-06 ルミネックス コーポレーション 不連続融解分析のための方法および組成物

Also Published As

Publication number Publication date
RU2011107993A (ru) 2012-09-20
WO2010017543A1 (en) 2010-02-11
EP2315844A1 (en) 2011-05-04
CN102112632A (zh) 2011-06-29
AU2009279457A1 (en) 2010-02-11
KR20110052681A (ko) 2011-05-18
BRPI0917434A2 (pt) 2015-12-01
US20110151461A1 (en) 2011-06-23
CA2733186A1 (en) 2010-02-11

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