RU2010129854A - Устройство и способ параллельного количественного анализа многочисленных нуклеиновых кислот - Google Patents
Устройство и способ параллельного количественного анализа многочисленных нуклеиновых кислот Download PDFInfo
- Publication number
- RU2010129854A RU2010129854A RU2010129854/10A RU2010129854A RU2010129854A RU 2010129854 A RU2010129854 A RU 2010129854A RU 2010129854/10 A RU2010129854/10 A RU 2010129854/10A RU 2010129854 A RU2010129854 A RU 2010129854A RU 2010129854 A RU2010129854 A RU 2010129854A
- Authority
- RU
- Russia
- Prior art keywords
- capture probes
- probes
- nucleic acid
- acid sequences
- target nucleic
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract 21
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract 21
- 108020004707 nucleic acids Proteins 0.000 title claims abstract 7
- 102000039446 nucleic acids Human genes 0.000 title claims abstract 7
- 238000004445 quantitative analysis Methods 0.000 title claims 2
- 239000000523 sample Substances 0.000 claims abstract 43
- 108091093088 Amplicon Proteins 0.000 claims abstract 12
- 230000003321 amplification Effects 0.000 claims abstract 6
- 239000007850 fluorescent dye Substances 0.000 claims abstract 6
- 238000009396 hybridization Methods 0.000 claims abstract 6
- 238000003199 nucleic acid amplification method Methods 0.000 claims abstract 6
- 230000007071 enzymatic hydrolysis Effects 0.000 claims abstract 3
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 claims abstract 3
- 108020005187 Oligonucleotide Probes Proteins 0.000 claims abstract 2
- 230000000295 complement effect Effects 0.000 claims abstract 2
- 239000002751 oligonucleotide probe Substances 0.000 claims abstract 2
- 239000011324 bead Substances 0.000 claims 6
- 238000006243 chemical reaction Methods 0.000 claims 3
- 239000011159 matrix material Substances 0.000 claims 2
- 150000001413 amino acids Chemical class 0.000 claims 1
- 238000000137 annealing Methods 0.000 claims 1
- 238000001514 detection method Methods 0.000 claims 1
- 239000000203 mixture Substances 0.000 claims 1
- 238000011895 specific detection Methods 0.000 claims 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP07123578A EP2077336A1 (en) | 2007-12-19 | 2007-12-19 | Device and method for parallel quantitative analysis of multiple nucleic acids |
| EP07123578.2 | 2007-12-19 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2010129854A true RU2010129854A (ru) | 2012-01-27 |
Family
ID=39118401
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2010129854/10A RU2010129854A (ru) | 2007-12-19 | 2008-12-16 | Устройство и способ параллельного количественного анализа многочисленных нуклеиновых кислот |
Country Status (6)
| Country | Link |
|---|---|
| US (1) | US20110039720A1 (https=) |
| EP (2) | EP2077336A1 (https=) |
| JP (1) | JP2011507503A (https=) |
| CN (1) | CN101903536A (https=) |
| RU (1) | RU2010129854A (https=) |
| WO (1) | WO2009077982A1 (https=) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| RU2011125332A (ru) * | 2008-11-21 | 2012-12-27 | Конинклейке Филипс Электроникс Н.В. | Определение множественной пцр в реальном времени на твердых поверхностях с использованием красителей со специфичностью к двухцепочечным нуклеиновым кислотам |
| US8703653B2 (en) | 2011-02-18 | 2014-04-22 | NVS Technologies, Inc. | Quantitative, highly multiplexed detection of nucleic acids |
| JP5216928B2 (ja) * | 2011-08-05 | 2013-06-19 | 株式会社東芝 | マルチ核酸増幅反応具 |
| WO2013021958A1 (ja) * | 2011-08-05 | 2013-02-14 | 株式会社 東芝 | マルチ核酸増幅反応具 |
| WO2013035867A1 (ja) * | 2011-09-08 | 2013-03-14 | 株式会社 東芝 | マルチ核酸反応具およびそれを用いた検出方法 |
| CN103998921B (zh) | 2011-10-14 | 2016-04-20 | 贝克顿·迪金森公司 | 方波热循环 |
| EP2780479A4 (en) * | 2011-11-17 | 2015-04-08 | Nvs Technologies Inc | QUANTITATIVE HIGHLY MULTIPLEXED DETECTION OF NUCLEIC ACIDS |
| EP2825309B1 (en) | 2012-03-16 | 2018-05-16 | Stat-Diagnostica & Innovation, S.L. | A test cartridge with integrated transfer module |
| ITTO20120302A1 (it) | 2012-04-05 | 2013-10-06 | St Microelectronics Srl | Dispositivo integrato e metodo per la reazione a catena della polimerasi quantitativa in tempo reale |
| WO2014028061A1 (en) * | 2012-08-16 | 2014-02-20 | NVS Technologies, Inc. | Assay methods and systems |
| EP2885431A4 (en) | 2012-08-17 | 2016-07-13 | Bio Nems Corp | CLASSIFICATION OF A NUCLEIC ACID |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US6346413B1 (en) | 1989-06-07 | 2002-02-12 | Affymetrix, Inc. | Polymer arrays |
| US5925517A (en) * | 1993-11-12 | 1999-07-20 | The Public Health Research Institute Of The City Of New York, Inc. | Detectably labeled dual conformation oligonucleotide probes, assays and kits |
| WO1997027317A1 (en) | 1996-01-23 | 1997-07-31 | Affymetrix, Inc. | Nucleic acid analysis techniques |
| US5861247A (en) | 1996-01-26 | 1999-01-19 | University Of Chicago | Rapid method to detect duplex formation in sequencing by hybridization methods |
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| WO2000061803A1 (en) * | 1999-04-13 | 2000-10-19 | Nanogen, Inc. | Magnetic bead-based array for genetic detection |
| DE60000583T3 (de) | 1999-05-19 | 2009-04-30 | Eppendorf Array Technologies | Verfahren zur identifizierung und/oder quantifizierung einer zielverbindung |
| EP1096024A1 (en) | 1999-10-28 | 2001-05-02 | Remacle, José | Method and kit for the screening and/or the quantification of multiple homologous nucleic acid sequences on arrays |
| CA2532220A1 (en) * | 2003-07-15 | 2005-01-27 | Daniel Henry Densham | Measurement of a polynucleotide amplification reaction |
| US20060088844A1 (en) * | 2004-10-22 | 2006-04-27 | Honeywell International Inc. | Real-time PCR microarray based on evanescent wave biosensor |
| JP2008543279A (ja) * | 2005-06-09 | 2008-12-04 | コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ | 磁気検出を伴った核酸の増幅 |
| DE102006005287B4 (de) * | 2006-02-06 | 2012-12-27 | Siemens Ag | Verfahren zum Nachweis von Zielnukleinsäuren |
-
2007
- 2007-12-19 EP EP07123578A patent/EP2077336A1/en not_active Ceased
-
2008
- 2008-12-16 CN CN2008801216256A patent/CN101903536A/zh active Pending
- 2008-12-16 EP EP08861093A patent/EP2235207A1/en not_active Withdrawn
- 2008-12-16 WO PCT/IB2008/055352 patent/WO2009077982A1/en not_active Ceased
- 2008-12-16 RU RU2010129854/10A patent/RU2010129854A/ru unknown
- 2008-12-16 US US12/809,412 patent/US20110039720A1/en not_active Abandoned
- 2008-12-16 JP JP2010539011A patent/JP2011507503A/ja not_active Withdrawn
Also Published As
| Publication number | Publication date |
|---|---|
| EP2077336A1 (en) | 2009-07-08 |
| WO2009077982A1 (en) | 2009-06-25 |
| US20110039720A1 (en) | 2011-02-17 |
| CN101903536A (zh) | 2010-12-01 |
| EP2235207A1 (en) | 2010-10-06 |
| JP2011507503A (ja) | 2011-03-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FA91 | Application withdrawn (on applicant's request) |
Effective date: 20130507 |