RU2003103771A - Способ генотипирования на микрочипах множества образцов по множественным локусам - Google Patents
Способ генотипирования на микрочипах множества образцов по множественным локусам Download PDFInfo
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- RU2003103771A RU2003103771A RU2003103771/13A RU2003103771A RU2003103771A RU 2003103771 A RU2003103771 A RU 2003103771A RU 2003103771/13 A RU2003103771/13 A RU 2003103771/13A RU 2003103771 A RU2003103771 A RU 2003103771A RU 2003103771 A RU2003103771 A RU 2003103771A
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- synthetic oligonucleotides
- microchip
- genomic fragments
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
Claims (25)
1. Способ одновременного генотипирования множества образцов, включающий амплификацию множества геномных фрагментов из множества образцов с помощью множества праймеров для полимеразной цепной реакции, причем каждый геномный фрагмент содержит отдельный генетический локус, формирование микрочипа из амплифицированных геномных фрагментов в виде матрицы на поверхности, причем каждая точка на этой поверхности содержит амплифицированный материал, происходящий из одного образца и состоящий практически из единственного геномного фрагмента, гибридизацию микрочипа со смесью меченых синтетических олигонуклеотидов, причем смесь включает олигонуклеотиды, комплементарные геномным фрагментам, получение генотипической информации одновременно для множества образцов по множеству генетических локусов путем детектирования сигналов с гибридизованного микрочипа, определяя тем самым генотип во множестве образцов.
2. Способ по п.1, в котором множество образцов включает не менее 10 отдельных образцов.
3. Способ по п.1, в котором множество образцов включает не менее 5000 отдельных образцов.
4. Способ по п.1, в котором геномные фрагменты включают локусы заболеваний человека.
5. Способ по п.4, в котором образцы представляют собой образцы крови новорожденных.
6. Способ по п.5, в котором генетические локусы включают локусы, связанные с геном человека, выбранным из группы, состоящей из β-глобина, CFTR и GALT.
7. Способ по п.1, в котором плотность микрочипа на поверхности составляет не менее 1000 точек на квадратный сантиметр.
8. Способ по п.1, в котором смесь меченых синтетических олигонуклеотидов включает 10 различных последовательностей олигонуклеотидов.
9. Способ по п.1, в котором меченые синтетические олигонуклеотиды имеют длину от 10 до 30 нуклеотидов.
10. Способ по п.1, в котором каждый из геномных фрагментов содержит от 40 до 1000 пар оснований.
11. Способ по п.1, в котором гибридизация осуществляется в водном растворе, содержащем соли и детергент.
12. Способ по п.1, в котором гибридизация осуществляется при температуре примерно на 10°С ниже температуры плавления меченых синтетических олигонуклеотидов.
13. Способ по п.1, в котором меченые синтетические олигонуклеотиды содержат флуоресцентные метки.
14. Способ по п.1, в котором меченые синтетические олигонуклеотиды содержат нефлуоресцентные метки.
15. Способ по п.1, в котором генотипическая информация позволяет отличить образцы из гомозигот от образцов из гетерозигот по конкретному генетическому локусу.
16. Способ по п.13, в котором сигналы возникают при испускании флуоресценции мечеными синтетическими олигонуклеотидами.
17. Способ по п.13, в котором сигналы возникают при испускании флуоресценции более чем на одной длине волны.
18. Способ по п.14, в котором сигналы возникают при испускании флуоресценции после окрашивания с помощью антител.
19. Способ по п.14, в котором сигналы возникают при испускании флуоресценции более чем на одной длине волны после окрашивания с помощью антител.
20. Способ по п.13, в котором флуоресцентные метки включают метки типа дендримеров.
21. Способ по п.1, в котором поверхность включает стекло.
22. Способ по п.1, в котором амплифицированные геномные фрагменты включают аминолинкеры.
23. Способ по п.22, в котором поверхность содержит реакционно-способные альдегидные группы.
24. Способ по п.1, в котором микрочип формируется путем механического микронанесения проб.
25. Способ одновременного генотипирования множества образцов, включающий амплификацию геномного фрагмента, содержащего генетический локус, из множества образцов с помощью праймеров для полимеразной цепной реакции, формирование микрочипа из амплифицированных геномных фрагментов в виде матрицы на поверхности, причем каждая точка на этой поверхности содержит материал, происходящий из одного образца, гибридизацию микрочипа со смесью меченых синтетических олигонуклеотидов, причем смесь включает олигонуклеотиды, комплементарные геномному фрагменту, получение генотипической информации одновременно для множества образцов путем детектирования сигналов с гибридизованного микрочипа, определяя тем самым генотип во множестве образцов.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/613,006 US6913879B1 (en) | 2000-07-10 | 2000-07-10 | Microarray method of genotyping multiple samples at multiple LOCI |
| US09/613,006 | 2000-07-10 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| RU2003103771A true RU2003103771A (ru) | 2004-08-20 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU2003103771/13A RU2003103771A (ru) | 2000-07-10 | 2001-07-03 | Способ генотипирования на микрочипах множества образцов по множественным локусам |
Country Status (17)
| Country | Link |
|---|---|
| US (2) | US6913879B1 (ru) |
| EP (1) | EP1343911B1 (ru) |
| JP (1) | JP4175884B2 (ru) |
| KR (1) | KR100756015B1 (ru) |
| CN (1) | CN100520401C (ru) |
| AT (1) | ATE363543T1 (ru) |
| AU (2) | AU1874002A (ru) |
| CA (1) | CA2414105C (ru) |
| DE (1) | DE60128720T2 (ru) |
| HK (1) | HK1057906A1 (ru) |
| IL (2) | IL153848A0 (ru) |
| IS (1) | IS2651B (ru) |
| NO (1) | NO20030101L (ru) |
| NZ (1) | NZ523560A (ru) |
| RU (1) | RU2003103771A (ru) |
| TW (1) | TWI280282B (ru) |
| WO (1) | WO2002003849A2 (ru) |
Families Citing this family (76)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10000001A1 (de) * | 2000-01-01 | 2001-07-19 | Agrobiogen Gmbh | DNA-Matrizes und deren Verwendung zur Untersuchung von Individuen einer Population |
| US6913879B1 (en) * | 2000-07-10 | 2005-07-05 | Telechem International Inc. | Microarray method of genotyping multiple samples at multiple LOCI |
| GB0208364D0 (en) * | 2002-04-11 | 2002-05-22 | Sec Dep For The Home Departmen | Improvements in and relating to methods and apparatus for genotyping |
| AU2003283575A1 (en) * | 2002-11-14 | 2004-06-03 | John Nicholas Housby | Polymorphism assay |
| JP4458327B2 (ja) * | 2003-08-28 | 2010-04-28 | キヤノン株式会社 | 標的物質の定量方法および該方法に用いるプローブ担体 |
| US7108979B2 (en) * | 2003-09-03 | 2006-09-19 | Agilent Technologies, Inc. | Methods to detect cross-contamination between samples contacted with a multi-array substrate |
| JP4742050B2 (ja) * | 2004-11-19 | 2011-08-10 | 株式会社島津製作所 | 検査試薬キットとそれを用いる遺伝子多型検出装置 |
| RU2348695C2 (ru) | 2006-05-23 | 2009-03-10 | Закрытое акционерное общество "Молекулярно-медицинские технологии" | Дифференцирующий и специфический олигонуклеотиды для идентификации последовательностей днк инфекционных агентов в биологических материалах, способ видовой идентификации инфекционных агентов, биочип и набор для осуществления этого способа |
| GB0618514D0 (en) * | 2006-09-20 | 2006-11-01 | Univ Nottingham Trent | Method of detecting interactions on a microarray using nuclear magnetic resonance |
| DK2099935T3 (da) * | 2006-11-30 | 2014-04-07 | Univ California | Array for detektion af mikrober |
| BRPI0811271A2 (pt) * | 2007-05-31 | 2015-01-20 | Monsanto Technology Llc | Polimorfismos da soja e processos de genotipagem |
| EP2735619A3 (en) | 2007-08-29 | 2014-08-13 | Monsanto Technology LLC | Methods and compositions for breeding for preferred traits associated with Goss' Wilt resistance in plants |
| EP4219762A1 (en) | 2008-02-01 | 2023-08-02 | The General Hospital Corporation | Use of microvesicles in diagnosis and prognosis of medical diseases and conditions |
| CN102098909B (zh) | 2008-04-24 | 2014-12-31 | 孟山都技术有限公司 | 鉴定大豆中亚洲大豆锈病抗性数量性状基因座的方法和其组合物 |
| US9393566B2 (en) * | 2008-06-23 | 2016-07-19 | Canon U.S. Life Sciences, Inc. | System and method for temperature referencing for melt curve data collection |
| EP2425240A4 (en) | 2009-04-30 | 2012-12-12 | Good Start Genetics Inc | METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS |
| US20100299773A1 (en) * | 2009-05-20 | 2010-11-25 | Monsanto Technology Llc | Methods and compositions for selecting an improved plant |
| EP2454588B1 (en) | 2009-07-16 | 2015-07-15 | The General Hospital Corporation | Nucleic acid analysis |
| US20130029339A1 (en) | 2009-09-09 | 2013-01-31 | The General Hospital Corporation | Use of microvesicles in analyzing kras mutations |
| EP2475988B1 (en) | 2009-09-09 | 2018-11-14 | The General Hospital Corporation | Use of microvesicles in analyzing nucleic acid profiles |
| US20140045915A1 (en) | 2010-08-31 | 2014-02-13 | The General Hospital Corporation | Cancer-related biological materials in microvesicles |
| US20130295574A1 (en) | 2010-11-10 | 2013-11-07 | Exosome Diagnostics, Inc. | Method for Isolation of Nucleic Acid Containing Particles and Extraction of Nucleic Acids Therefrom |
| US9163281B2 (en) | 2010-12-23 | 2015-10-20 | Good Start Genetics, Inc. | Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction |
| US10172305B2 (en) | 2011-04-29 | 2019-01-08 | Monsanto Technology Llc | Diagnostic molecular markers for seed lot purity traits in soybeans |
| AU2012253366A1 (en) | 2011-05-11 | 2014-01-09 | Exosome Diagnostics, Inc. | Nucleic acid extraction from heterogeneous biological materials |
| EP3492606B1 (en) | 2011-08-22 | 2023-10-04 | Exosome Diagnostics, Inc. | Urine biomarkers |
| HUE044521T2 (hu) | 2011-08-31 | 2019-10-28 | Seminis Vegetable Seeds Inc | Görögdinnye szilárdságával kapcsolatos eljárások és kompozíciók |
| US9228233B2 (en) | 2011-10-17 | 2016-01-05 | Good Start Genetics, Inc. | Analysis methods |
| US10174361B2 (en) | 2011-11-10 | 2019-01-08 | Exosome Diagnostics, Inc. | Cerebrospinal fluid assay |
| WO2013106737A1 (en) * | 2012-01-13 | 2013-07-18 | Data2Bio | Genotyping by next-generation sequencing |
| US8209130B1 (en) | 2012-04-04 | 2012-06-26 | Good Start Genetics, Inc. | Sequence assembly |
| US8812422B2 (en) | 2012-04-09 | 2014-08-19 | Good Start Genetics, Inc. | Variant database |
| US10227635B2 (en) | 2012-04-16 | 2019-03-12 | Molecular Loop Biosolutions, Llc | Capture reactions |
| EP2890783A4 (en) | 2012-08-30 | 2016-03-02 | Exosome Diagnostics Inc | CONTROLS FOR NUCLEIC ACID TESTS |
| CN105025878A (zh) | 2012-10-03 | 2015-11-04 | 外来体诊断公司 | 微泡在医学疾病和病况的诊断、预后和治疗中的用途 |
| EP2917368A1 (en) | 2012-11-07 | 2015-09-16 | Good Start Genetics, Inc. | Methods and systems for identifying contamination in samples |
| GB201220924D0 (en) | 2012-11-21 | 2013-01-02 | Cancer Res Inst Royal | Materials and methods for determining susceptibility or predisposition to cancer |
| CN104797938B (zh) * | 2012-12-03 | 2017-12-26 | 国家血清研究所 | 在新生儿的筛查中诊断半乳糖血症的方法 |
| US10314253B2 (en) | 2012-12-04 | 2019-06-11 | Seminis Vegetable Seeds, Inc. | Methods and compositions for watermelon sex expression |
| EP2971159B1 (en) | 2013-03-14 | 2019-05-08 | Molecular Loop Biosolutions, LLC | Methods for analyzing nucleic acids |
| US10294489B2 (en) | 2013-03-15 | 2019-05-21 | Board Of Trustees Of Southern Illinois University | Soybean resistant to cyst nematodes |
| EP3005200A2 (en) | 2013-06-03 | 2016-04-13 | Good Start Genetics, Inc. | Methods and systems for storing sequence read data |
| US10059999B2 (en) | 2013-06-10 | 2018-08-28 | Monsanto Technology Llc | Molecular markers associated with soybean tolerance to low iron growth conditions |
| RU2668164C2 (ru) | 2013-08-06 | 2018-09-26 | Эксосам Дайэгностикс, Инк. | Когорты биомаркеров мочи, профиль экспрессии генов и способы их применения |
| US10851414B2 (en) | 2013-10-18 | 2020-12-01 | Good Start Genetics, Inc. | Methods for determining carrier status |
| US11041203B2 (en) | 2013-10-18 | 2021-06-22 | Molecular Loop Biosolutions, Inc. | Methods for assessing a genomic region of a subject |
| NZ735461A (en) | 2013-11-27 | 2020-05-29 | Seminis Vegetable Seeds Inc | Disease resistance loci in onion |
| UA122122C2 (uk) | 2014-02-21 | 2020-09-25 | Сінгента Партісіпейшнс Аг | Спосіб виявлення рослини маїсу, що має підвищену чоловічу фертильність |
| NZ630710A (en) | 2014-02-27 | 2016-03-31 | Seminis Vegetable Seeds Inc | Compositions and methods for peronospora resistance in spinach |
| WO2015175530A1 (en) | 2014-05-12 | 2015-11-19 | Gore Athurva | Methods for detecting aneuploidy |
| US10316369B2 (en) | 2014-06-27 | 2019-06-11 | Seminis Vegetable Seeds, Inc. | Methods and assays for male sterile watermelon |
| WO2016040446A1 (en) | 2014-09-10 | 2016-03-17 | Good Start Genetics, Inc. | Methods for selectively suppressing non-target sequences |
| CA2999708A1 (en) | 2014-09-24 | 2016-03-31 | Good Start Genetics, Inc. | Process control for increased robustness of genetic assays |
| EP3005862A1 (en) | 2014-10-10 | 2016-04-13 | Seminis Vegetable Seeds, Inc. | Melon plants with improved disease tolerance |
| US10066259B2 (en) | 2015-01-06 | 2018-09-04 | Good Start Genetics, Inc. | Screening for structural variants |
| HUE053541T2 (hu) | 2015-04-28 | 2021-07-28 | Monsanto Technology Llc | Eljárások és készítmények brachitikus kukoricanövények elõállítására |
| EP3288369A4 (en) | 2015-04-30 | 2018-09-12 | Monsanto Technology LLC | Methods for producing canola plants with clubroot resistance and compositions thereof |
| CR20180164A (es) | 2015-08-18 | 2018-09-04 | Monsanto Technology Llc | Métodos para producir plantas de algodón con tolerancia mejorada a la sequía y composiciones de estas |
| US10448595B2 (en) | 2015-09-03 | 2019-10-22 | Seminis Vegetable Seeds, Inc. | Downy mildew resistant lettuce plants |
| WO2017044744A2 (en) | 2015-09-10 | 2017-03-16 | Monsanto Technology Llc | Methods for producing corn plants with downy mildew resistance and compositions thereof |
| BR112018012039A2 (pt) | 2015-12-18 | 2018-12-04 | Monsanto Technology Llc | métodos para produção de plantas de milho com resistência à helmintosporiose e composições dos mesmos |
| EP3443117A1 (en) | 2016-04-15 | 2019-02-20 | Exosome Diagnostics, Inc. | Plasma-based detection of anaplastic lymphoma kinase (alk) nucleic acids and alk fusion transcripts and uses thereof in diagnosis and treatment of cancer |
| CN109642229A (zh) | 2016-05-13 | 2019-04-16 | 外来体诊断公司 | 从生物液体分离细胞外囊泡和共分离无细胞dna的自动和手动方法 |
| WO2018031874A1 (en) | 2016-08-11 | 2018-02-15 | Monsanto Technology Llc | Methods and compositions for producing corn plants with resistance to late wilt |
| AU2017232187B2 (en) | 2016-09-30 | 2023-11-09 | Seminis Vegetable Seeds, Inc. | Xanthomonas resistant brassica oleracea plants |
| EP3529374B1 (en) | 2016-10-21 | 2024-04-03 | Exosome Diagnostics, Inc. | Sequencing and analysis of exosome associated nucleic acids |
| CN110446790B (zh) | 2016-11-30 | 2023-03-31 | 外来体诊断公司 | 使用外来体rna和无细胞dna检测血浆中的突变的方法和组合物 |
| US20200149036A1 (en) | 2017-01-02 | 2020-05-14 | Exosome Diagnostics, Inc. | Methods to distinguish rna and dna in a combined preparation |
| EP3652315A4 (en) | 2017-07-12 | 2021-09-01 | Exosome Diagnostics, Inc. | METHOD OF ISOLATION AND ENRICHMENT OF POPULATIONS OF EXTRACELLULAR VESICULES FROM A BIOFLUID AND METHOD OF USING THEREOF |
| US11268102B2 (en) | 2018-05-16 | 2022-03-08 | University Of Florida Research Foundation, Incorporated | Compositions and methods for identifying and selecting brachytic locus in solanaceae |
| EP3884045B1 (en) | 2018-11-20 | 2023-01-11 | Exosome Diagnostics, Inc. | Methods for internal controls of microvesicle isolations |
| AU2020208190B2 (en) | 2019-01-15 | 2022-07-28 | Seminis Vegetable Seeds, Inc. | Green bean plants with improved disease resistance |
| SG10202008262UA (en) | 2019-09-26 | 2021-04-29 | Seminis Vegetable Seeds Inc | Lettuce plants having resistance to nasonovia ribisnigri biotype nr:1 |
| AU2021204717A1 (en) | 2020-07-15 | 2022-02-03 | Seminis Vegetable Seeds, Inc. | Green Bean Plants with Improved Disease Resistance |
| US20230193310A1 (en) | 2021-12-10 | 2023-06-22 | Seminis Vegetabe Seeds, Inc. | Lettuce plants having resistance to downy mildew |
| WO2023250288A2 (en) | 2022-06-21 | 2023-12-28 | Seminis Vegetable Seeds, Inc. | Novel qtls conferring resistance to cucumber mosaic virus |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4981783A (en) | 1986-04-16 | 1991-01-01 | Montefiore Medical Center | Method for detecting pathological conditions |
| US6270961B1 (en) | 1987-04-01 | 2001-08-07 | Hyseq, Inc. | Methods and apparatus for DNA sequencing and DNA identification |
| US5202231A (en) | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
| US5700637A (en) | 1988-05-03 | 1997-12-23 | Isis Innovation Limited | Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5925525A (en) | 1989-06-07 | 1999-07-20 | Affymetrix, Inc. | Method of identifying nucleotide differences |
| US5800992A (en) * | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
| AU4292893A (en) | 1992-04-21 | 1993-11-18 | Regents Of The University Of California, The | Multicolor in situ hybridization methods for genetic testing |
| US5403708A (en) | 1992-07-06 | 1995-04-04 | Brennan; Thomas M. | Methods and compositions for determining the sequence of nucleic acids |
| US5858659A (en) | 1995-11-29 | 1999-01-12 | Affymetrix, Inc. | Polymorphism detection |
| US6045996A (en) | 1993-10-26 | 2000-04-04 | Affymetrix, Inc. | Hybridization assays on oligonucleotide arrays |
| US5807522A (en) | 1994-06-17 | 1998-09-15 | The Board Of Trustees Of The Leland Stanford Junior University | Methods for fabricating microarrays of biological samples |
| US5834181A (en) | 1994-07-28 | 1998-11-10 | Genzyme Corporation | High throughput screening method for sequences or genetic alterations in nucleic acids |
| US5830645A (en) | 1994-12-09 | 1998-11-03 | The Regents Of The University Of California | Comparative fluorescence hybridization to nucleic acid arrays |
| US5545531A (en) | 1995-06-07 | 1996-08-13 | Affymax Technologies N.V. | Methods for making a device for concurrently processing multiple biological chip assays |
| US5958342A (en) | 1996-05-17 | 1999-09-28 | Incyte Pharmaceuticals, Inc. | Jet droplet device |
| US6057100A (en) | 1996-06-07 | 2000-05-02 | Eos Biotechnology, Inc. | Oligonucleotide arrays |
| US5919626A (en) | 1997-06-06 | 1999-07-06 | Orchid Bio Computer, Inc. | Attachment of unmodified nucleic acids to silanized solid phase surfaces |
| US6013449A (en) | 1997-11-26 | 2000-01-11 | The United States Of America As Represented By The Department Of Health And Human Services | Probe-based analysis of heterozygous mutations using two-color labelling |
| US6232066B1 (en) | 1997-12-19 | 2001-05-15 | Neogen, Inc. | High throughput assay system |
| US6238869B1 (en) | 1997-12-19 | 2001-05-29 | High Throughput Genomics, Inc. | High throughput assay system |
| AU6163399A (en) | 1998-09-25 | 2000-04-17 | Massachusetts Institute Of Technology | Methods and products related to genotyping and dna analysis |
| US6268147B1 (en) * | 1998-11-02 | 2001-07-31 | Kenneth Loren Beattie | Nucleic acid analysis using sequence-targeted tandem hybridization |
| US7285384B2 (en) * | 2000-02-16 | 2007-10-23 | Illuminia, Inc. | Parallel genotyping of multiple patient samples |
| US6913879B1 (en) * | 2000-07-10 | 2005-07-05 | Telechem International Inc. | Microarray method of genotyping multiple samples at multiple LOCI |
-
2000
- 2000-07-10 US US09/613,006 patent/US6913879B1/en not_active Expired - Fee Related
-
2001
- 2001-07-03 AU AU1874002A patent/AU1874002A/xx active Pending
- 2001-07-03 EP EP01984136A patent/EP1343911B1/en not_active Expired - Lifetime
- 2001-07-03 IL IL15384801A patent/IL153848A0/xx unknown
- 2001-07-03 DE DE60128720T patent/DE60128720T2/de not_active Expired - Lifetime
- 2001-07-03 JP JP2002508311A patent/JP4175884B2/ja not_active Expired - Fee Related
- 2001-07-03 KR KR1020037000360A patent/KR100756015B1/ko not_active IP Right Cessation
- 2001-07-03 AU AU2002218740A patent/AU2002218740B2/en not_active Ceased
- 2001-07-03 AT AT01984136T patent/ATE363543T1/de not_active IP Right Cessation
- 2001-07-03 CN CNB018139728A patent/CN100520401C/zh not_active Expired - Fee Related
- 2001-07-03 NZ NZ523560A patent/NZ523560A/en unknown
- 2001-07-03 CA CA2414105A patent/CA2414105C/en not_active Expired - Fee Related
- 2001-07-03 RU RU2003103771/13A patent/RU2003103771A/ru not_active Application Discontinuation
- 2001-07-03 WO PCT/US2001/021163 patent/WO2002003849A2/en active IP Right Grant
- 2001-07-09 TW TW090116726A patent/TWI280282B/zh not_active IP Right Cessation
-
2003
- 2003-01-08 IL IL153848A patent/IL153848A/en not_active IP Right Cessation
- 2003-01-09 IS IS6674A patent/IS2651B/is unknown
- 2003-01-09 NO NO20030101A patent/NO20030101L/no not_active Application Discontinuation
-
2004
- 2004-01-31 HK HK04100681A patent/HK1057906A1/xx not_active IP Right Cessation
- 2004-11-05 US US10/981,829 patent/US20050153318A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CN100520401C (zh) | 2009-07-29 |
| WO2002003849A2 (en) | 2002-01-17 |
| CN1636137A (zh) | 2005-07-06 |
| IS6674A (is) | 2003-01-09 |
| CA2414105A1 (en) | 2002-01-17 |
| AU1874002A (en) | 2002-01-21 |
| KR100756015B1 (ko) | 2007-09-07 |
| DE60128720T2 (de) | 2008-01-24 |
| IL153848A (en) | 2009-09-22 |
| US20050153318A1 (en) | 2005-07-14 |
| NZ523560A (en) | 2006-01-27 |
| JP2004502441A (ja) | 2004-01-29 |
| NO20030101L (no) | 2003-03-07 |
| DE60128720D1 (de) | 2007-07-12 |
| HK1057906A1 (en) | 2004-04-23 |
| JP4175884B2 (ja) | 2008-11-05 |
| KR20030040352A (ko) | 2003-05-22 |
| TWI280282B (en) | 2007-05-01 |
| IS2651B (is) | 2010-08-15 |
| ATE363543T1 (de) | 2007-06-15 |
| WO2002003849A3 (en) | 2003-06-19 |
| US6913879B1 (en) | 2005-07-05 |
| EP1343911B1 (en) | 2007-05-30 |
| CA2414105C (en) | 2011-07-12 |
| EP1343911A2 (en) | 2003-09-17 |
| IL153848A0 (en) | 2003-07-31 |
| NO20030101D0 (no) | 2003-01-09 |
| AU2002218740B2 (en) | 2007-03-22 |
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| FA92 | Acknowledgement of application withdrawn (lack of supplementary materials submitted) |
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