RS63202B1 - Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija - Google Patents

Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija

Info

Publication number
RS63202B1
RS63202B1 RS20220453A RSP20220453A RS63202B1 RS 63202 B1 RS63202 B1 RS 63202B1 RS 20220453 A RS20220453 A RS 20220453A RS P20220453 A RSP20220453 A RS P20220453A RS 63202 B1 RS63202 B1 RS 63202B1
Authority
RS
Serbia
Prior art keywords
instruments
modules
systems
processing methods
cell processing
Prior art date
Application number
RS20220453A
Other languages
English (en)
Inventor
Don Masquelier
Phillip Belgrader
Jorge Bernate
Ryan Gill
Kevin Ness
Original Assignee
Inscripta Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inscripta Inc filed Critical Inscripta Inc
Publication of RS63202B1 publication Critical patent/RS63202B1/sr

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M43/00—Combinations of bioreactors or fermenters with other apparatus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/44—Multiple separable units; Modules
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/36—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of biomass, e.g. colony counters or by turbidity measurements
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6865—Promoter-based amplification, e.g. nucleic acid sequence amplification [NASBA], self-sustained sequence replication [3SR] or transcription-based amplification system [TAS]
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites
RS20220453A 2017-06-30 2018-06-30 Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija RS63202B1 (sr)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201762527339P 2017-06-30 2017-06-30
US201762551069P 2017-08-28 2017-08-28
US201762566374P 2017-09-30 2017-09-30
US201762566375P 2017-09-30 2017-09-30
US201762566688P 2017-10-02 2017-10-02
US201762567697P 2017-10-03 2017-10-03
US201862620370P 2018-01-22 2018-01-22
US201862648130P 2018-03-26 2018-03-26
US201862649731P 2018-03-29 2018-03-29
US201862657654P 2018-04-13 2018-04-13
US201862657651P 2018-04-13 2018-04-13
US201862671385P 2018-05-14 2018-05-14
US201862689068P 2018-06-23 2018-06-23
EP18825038.5A EP3645719B1 (en) 2017-06-30 2018-06-30 Automated cell processing methods, modules, instruments, and systems
PCT/US2018/040519 WO2019006436A1 (en) 2017-06-30 2018-06-30 METHODS, MODULES, INSTRUMENTS AND SYSTEMS FOR AUTOMATED CELL PROCESSING

Publications (1)

Publication Number Publication Date
RS63202B1 true RS63202B1 (sr) 2022-06-30

Family

ID=64734781

Family Applications (1)

Application Number Title Priority Date Filing Date
RS20220453A RS63202B1 (sr) 2017-06-30 2018-06-30 Automatizovani postupci, moduli, instrumenti i sistemi za obradu ćelija

Country Status (20)

Country Link
US (20) US20190002874A1 (sr)
EP (3) EP4270012A3 (sr)
JP (1) JP2020530979A (sr)
KR (2) KR102424850B1 (sr)
CN (1) CN111094567A (sr)
AU (2) AU2018291041B2 (sr)
CA (1) CA3066253C (sr)
DK (1) DK3645719T3 (sr)
ES (1) ES2913457T3 (sr)
HR (1) HRP20220615T1 (sr)
HU (1) HUE058668T2 (sr)
IL (1) IL271196B (sr)
LT (1) LT3645719T (sr)
MX (1) MX2019015505A (sr)
PL (1) PL3645719T3 (sr)
PT (1) PT3645719T (sr)
RS (1) RS63202B1 (sr)
RU (2) RU2755308C2 (sr)
SI (1) SI3645719T1 (sr)
WO (1) WO2019006436A1 (sr)

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103998394B (zh) 2011-08-01 2016-08-17 德诺弗科学公司 细胞捕获系统和使用方法
US9404864B2 (en) 2013-03-13 2016-08-02 Denovo Sciences, Inc. System for imaging captured cells
US10466160B2 (en) 2011-08-01 2019-11-05 Celsee Diagnostics, Inc. System and method for retrieving and analyzing particles
US10391490B2 (en) 2013-05-31 2019-08-27 Celsee Diagnostics, Inc. System and method for isolating and analyzing cells
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
US11208649B2 (en) 2015-12-07 2021-12-28 Zymergen Inc. HTP genomic engineering platform
US11293021B1 (en) 2016-06-23 2022-04-05 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US20190002874A1 (en) 2017-06-30 2019-01-03 Inscripta, Inc. Cell libraries created using rationally designed nucleic acids
US10738327B2 (en) * 2017-08-28 2020-08-11 Inscripta, Inc. Electroporation cuvettes for automation
EP3651903A4 (en) 2017-08-29 2021-06-16 Bio-Rad Laboratories, Inc. SYSTEM AND METHOD FOR ISOLATING AND ANALYZING CELLS
CN114958772A (zh) 2017-09-01 2022-08-30 隆萨沃克斯维尔股份有限公司 端到端细胞治疗自动化
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
WO2019209926A1 (en) * 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10858761B2 (en) 2018-04-24 2020-12-08 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US10526598B2 (en) 2018-04-24 2020-01-07 Inscripta, Inc. Methods for identifying T-cell receptor antigens
EP3790607B1 (en) 2018-05-11 2023-12-27 Lupagen, Inc. Systems for closed loop, real-time modifications of patient cells
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US10532324B1 (en) 2018-08-14 2020-01-14 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
US11142740B2 (en) 2018-08-14 2021-10-12 Inscripta, Inc. Detection of nuclease edited sequences in automated modules and instruments
CN112955540A (zh) 2018-08-30 2021-06-11 因思科瑞普特公司 在自动化模块和仪器中对经核酸酶经编辑的序列的改进的检测
US10775395B2 (en) * 2018-10-18 2020-09-15 Arctoris Limited System and method of performing a biological experiment with adaptive cybernetic control of procedural conditions
US10604746B1 (en) 2018-10-22 2020-03-31 Inscripta, Inc. Engineered enzymes
US11214781B2 (en) 2018-10-22 2022-01-04 Inscripta, Inc. Engineered enzyme
EP3894540A4 (en) * 2018-12-11 2022-11-16 Lonza Walkersville, Inc. ISOLATION OF CELLS FOR USE IN AUTOMATED BIOREACTORS
US11714096B2 (en) 2018-12-21 2023-08-01 Octane Biotech Inc. Carousel for modular biologic production units
SG11202106377XA (en) 2018-12-21 2021-07-29 Lonza Walkersville Inc Automated production of viral vectors
KR20210125510A (ko) 2019-02-08 2021-10-18 론자 워커스빌 아이엔씨. 자동화 생물반응기에서 사용하기 위한 세포 농축 방법 및 장치
WO2020180506A1 (en) * 2019-02-25 2020-09-10 Inscripta, Inc. Nucleic acid-guided editing of exogenous polynucleotides in heterologous cells
US11001831B2 (en) 2019-03-25 2021-05-11 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
AU2020247900A1 (en) 2019-03-25 2021-11-04 Inscripta, Inc. Simultaneous multiplex genome editing in yeast
US10633693B1 (en) 2019-04-16 2020-04-28 Celsee Diagnostics, Inc. System and method for leakage control in a particle capture system
US11273439B2 (en) 2019-05-07 2022-03-15 Bio-Rad Laboratories, Inc. System and method for target material retrieval from microwells
CA3138881A1 (en) * 2019-05-07 2020-11-12 Bio-Rad Laboratories, Inc. System and method for automated single cell processing
US10837021B1 (en) 2019-06-06 2020-11-17 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
AU2020287882A1 (en) * 2019-06-07 2022-01-20 Juno Therapeutics, Inc. Automated T cell culture
US11724256B2 (en) 2019-06-14 2023-08-15 Bio-Rad Laboratories, Inc. System and method for automated single cell processing and analyses
US10920189B2 (en) 2019-06-21 2021-02-16 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced lysine production in E. coli
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US20210010006A1 (en) * 2019-07-08 2021-01-14 Inscripta, Inc. Increased nucleic acid-guided cell editing via a lexa-rad51 fusion protein
EP4058563A4 (en) * 2019-11-11 2023-12-06 Lonza Walkersville, Inc. QUALITY CONTROL PROCEDURES FOR AUTOMATED CELL PROCESSING
US11203762B2 (en) 2019-11-19 2021-12-21 Inscripta, Inc. Methods for increasing observed editing in bacteria
CN114787347A (zh) 2019-12-10 2022-07-22 因思科瑞普特公司 新颖的mad核酸酶
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
JP2023507566A (ja) 2019-12-18 2023-02-24 インスクリプタ， インコーポレイテッド 核酸誘導ヌクレアーゼ編集済み細胞のｉｎ ｖｉｖｏ検出のためのカスケード／ｄＣａｓ３相補性アッセイ
CA3159479A1 (en) * 2019-12-19 2021-06-24 Lonza Walkersville, Inc. Automated production of viral vectors
US10689669B1 (en) 2020-01-11 2020-06-23 Inscripta, Inc. Automated multi-module cell processing methods, instruments, and systems
CN115279899A (zh) * 2020-01-11 2022-11-01 因思科瑞普特公司 具有合理设计的编辑的细胞群体
CA3157061A1 (en) 2020-01-27 2021-08-05 Christian SILTANEN Electroporation modules and instrumentation
WO2021183687A2 (en) 2020-03-10 2021-09-16 Cellares Corporation Systems, devices, and methods for cell processing
US20210332388A1 (en) 2020-04-24 2021-10-28 Inscripta, Inc. Compositions, methods, modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells
US11787841B2 (en) 2020-05-19 2023-10-17 Inscripta, Inc. Rationally-designed mutations to the thrA gene for enhanced lysine production in E. coli
CN111949058B (zh) * 2020-07-29 2022-02-08 北京擎科生物科技有限公司 室内温度与dna合成仪内湿度的联动控制系统及方法
US11299731B1 (en) 2020-09-15 2022-04-12 Inscripta, Inc. CRISPR editing to embed nucleic acid landing pads into genomes of live cells
US11512297B2 (en) 2020-11-09 2022-11-29 Inscripta, Inc. Affinity tag for recombination protein recruitment
EP4271802A1 (en) 2021-01-04 2023-11-08 Inscripta, Inc. Mad nucleases
EP4274890A1 (en) 2021-01-07 2023-11-15 Inscripta, Inc. Mad nucleases
US11884924B2 (en) 2021-02-16 2024-01-30 Inscripta, Inc. Dual strand nucleic acid-guided nickase editing
EP4334706A1 (en) * 2021-05-05 2024-03-13 Singular Genomics Systems, Inc. Multiomic analysis device and methods of use thereof
RU209703U1 (ru) * 2021-07-15 2022-03-18 Федеральное государственное автономное образовательное учреждение высшего образования «Дальневосточный федеральный университет» (ДВФУ) Инкубационная установка для длительного содержания переживающих срезов
WO2023014850A1 (en) * 2021-08-05 2023-02-09 Inscripta, Inc. Modules and instruments for automated nucleic acid-guided nuclease editing in mammalian cells using microcarriers
WO2023028521A1 (en) 2021-08-24 2023-03-02 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced cellobiohydrolase i production in s. cerevisiae
US11530406B1 (en) 2021-08-30 2022-12-20 Sachi Bioworks Inc. System and method for producing a therapeutic oligomer
WO2023102481A1 (en) 2021-12-02 2023-06-08 Inscripta, Inc. Trackable nucleic acid-guided editing
WO2023122023A1 (en) 2021-12-20 2023-06-29 Inscripta, Inc. Targeted genomic barcoding for tracking of editing events
US20240052370A1 (en) 2022-07-27 2024-02-15 Inscripta, Inc. Modulating cellular repair mechanisms for genomic editing
GB2621626A (en) * 2022-08-19 2024-02-21 Oribiotech Ltd A modular bioprocessing system

Family Cites Families (235)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US1776008A (en) 1929-07-24 1930-09-16 James H Plater Piston-ring expander
CA1293460C (en) 1985-10-07 1991-12-24 Brian Lee Sauer Site-specific recombination of dna in yeast
US4833080A (en) 1985-12-12 1989-05-23 President And Fellows Of Harvard College Regulation of eucaryotic gene expression
US6022742A (en) 1986-11-26 2000-02-08 Kopf; Henry B. Culture device and method
US5464764A (en) 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US6689610B1 (en) 1989-08-22 2004-02-10 University Of Utah Research Foundation Cells and non-human organisms containing predetermined genomic modifications and positive-negative selection methods and vectors for making same
KR100236506B1 (ko) 1990-11-29 2000-01-15 퍼킨-엘머시터스인스트루먼츠 폴리머라제 연쇄 반응 수행 장치
WO1992015694A1 (en) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Flp-mediated gene modification in mammalian cells, and compositions and cells useful therefor
US6074605A (en) 1995-03-10 2000-06-13 Entremed, Inc. Flow electroporation chamber and method
NZ312332A (en) 1995-06-07 2000-01-28 Life Technologies Inc Recombinational cloning using engineered recombination sites
US5851804A (en) 1996-05-06 1998-12-22 Apollon, Inc. Chimeric kanamycin resistance gene
US5792943A (en) 1997-04-30 1998-08-11 Hewlett-Packard Company Planar separation column for use in sample analysis system
US6774279B2 (en) 1997-05-30 2004-08-10 Carnegie Institution Of Washington Use of FLP recombinase in mice
EP1025217B1 (en) 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
DK1034260T3 (da) 1997-12-05 2003-09-29 Europ Lab Molekularbiolog Hidtil ukendt DNA-kloningsmetode under anvendelse af E.coli-RECE/RECT-rekombinationssystemet
US6027488A (en) 1998-06-03 2000-02-22 Genetronics, Inc. Flow-through electroporation system for ex vivo gene therapy
EP2428250A1 (en) 1998-07-13 2012-03-14 Genetronics, Inc. Skin and muscle-targeted gene therapy by pulsed electrical field
US6391582B2 (en) 1998-08-14 2002-05-21 Rigel Pharmaceuticlas, Inc. Shuttle vectors
US6150148A (en) 1998-10-21 2000-11-21 Genetronics, Inc. Electroporation apparatus for control of temperature during the process
DE19859459A1 (de) * 1998-12-22 2000-06-29 Evotec Biosystems Ag Mikrosysteme zur Zellpermeation und Zellfusion
EP1147209A2 (en) 1999-02-03 2001-10-24 The Children's Medical Center Corporation Gene repair involving the induction of double-stranded dna cleavage at a chromosomal target site
SE9900530D0 (sv) 1999-02-15 1999-02-15 Vincenzo Vassarotti A device for concentrating and/or purifying macromolecules in a solution and a method for manufacturing such a device
US6678558B1 (en) 1999-03-25 2004-01-13 Genetronics, Inc. Method and apparatus for reducing electroporation-mediated muscle reaction and pain response
JP4495866B2 (ja) * 1999-05-28 2010-07-07 セフィード 化学反応を制御するためのカートリッジ
US6818185B1 (en) * 1999-05-28 2004-11-16 Cepheid Cartridge for conducting a chemical reaction
US6300108B1 (en) 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US6986993B1 (en) 1999-08-05 2006-01-17 Cellomics, Inc. System for cell-based screening
AU2001280968A1 (en) 2000-07-31 2002-02-13 Menzel, Rolf Compositions and methods for directed gene assembly
JP5043277B2 (ja) 2000-08-21 2012-10-10 ライフ テクノロジーズ コーポレーション 分子クローニング法および使用試薬
FR2814642B1 (fr) 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
US20020139741A1 (en) 2001-03-27 2002-10-03 Henry Kopf Integral gasketed filtration cassette article and method of making the same
WO2003018751A2 (en) 2001-08-22 2003-03-06 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US7166443B2 (en) 2001-10-11 2007-01-23 Aviva Biosciences Corporation Methods, compositions, and automated systems for separating rare cells from fluid samples
TWI229696B (en) * 2001-11-09 2005-03-21 Yeastern Biotech Co Ltd A fast method of transforming competent cells
US7018819B2 (en) 2001-11-30 2006-03-28 Cellectricon Ab Method and apparatus for manipulation of cells and cell-like structures focused electric fields in microfludic systems and use thereof
AU2003206415A1 (en) 2002-01-07 2003-07-24 Uab Research Foundation Electroporation cuvette-pipette tips, multi-well cuvette arrays, and electrode template apparatus adapted for automation and uses thereof
AU2003251286B2 (en) 2002-01-23 2007-08-16 The University Of Utah Research Foundation Targeted chromosomal mutagenesis using zinc finger nucleases
EP1506413B1 (en) * 2002-05-17 2016-07-06 Becton Dickinson and Company Automated system for isolating, amplyifying and detecting a target nucleic acid sequence
CA2391641A1 (fr) 2002-06-28 2003-12-28 Robert Longin Plateforme robotisee de cultures cellulaires en batteries de reacteurs miniaturises, equipee d'un systeme de mesure en temps reel de la turbidite cellulaire ou toutes autres proprietes optiques
US20040110253A1 (en) 2002-08-13 2004-06-10 Kappler John W. Method for identifying MHC-presented peptide epitopes for T cells
AU2003277109A1 (en) 2002-09-30 2004-04-23 Maxcyte, Inc. Apparatus and method for streaming electroporation
US20040138154A1 (en) 2003-01-13 2004-07-15 Lei Yu Solid surface for biomolecule delivery and high-throughput assay
EP1516920A1 (en) 2003-09-19 2005-03-23 The Automation Partnership Cell culture vessel for the automated processing of cell cultures
US20050118705A1 (en) 2003-11-07 2005-06-02 Rabbitt Richard D. Electrical detectors for microanalysis
US7078227B2 (en) 2004-03-26 2006-07-18 Molecular Transfer Ready-to-use electroporation cuvette including frozen electrocompetent cells
DK1766057T3 (en) 2004-05-12 2015-03-02 Maxcyte Inc Methods and devices related to a regulated flowelektroporationskammer
AU2005257583A1 (en) 2004-06-12 2006-01-05 Digital Bio Technology Co., Ltd. Electroporator having an elongated hollow member
US7422889B2 (en) 2004-10-29 2008-09-09 Stowers Institute For Medical Research Dre recombinase and recombinase systems employing Dre recombinase
EP1869476A2 (en) * 2005-03-22 2007-12-26 Irm Llc Compound profiling devices, systems, and related methods
WO2006130299A2 (en) 2005-05-03 2006-12-07 Micronics, Inc. Microfluidic laminar flow detection strip
PT1916903E (pt) 2005-08-26 2010-09-03 Danisco Utilização de genes associados a crispr (cas)
US20070105206A1 (en) 2005-10-19 2007-05-10 Chang Lu Fluidic device
US20110189650A1 (en) 2010-02-03 2011-08-04 Ayliffe Harold E Microfluidic cell sorter with electroporation
US7923238B2 (en) 2006-02-10 2011-04-12 Bio-Rad Laboratories, Inc. Multi-channel electroporation system
US7799555B2 (en) 2006-02-10 2010-09-21 Bio-Rad Laboratories, Inc. Apparatus for high-throughput electroporation
WO2008052101A2 (en) 2006-10-25 2008-05-02 President And Fellows Of Harvard College Multiplex automated genome engineering
WO2008060521A1 (en) 2006-11-14 2008-05-22 Acme Biosystems, Llc Cell culture apparatus and associated methods
US8748146B2 (en) 2007-04-19 2014-06-10 Celexion, Llc Engineered nucleases and their uses for nucleic acid assembly
US20110213288A1 (en) 2007-04-23 2011-09-01 The Board Of Regents, The University Of Texas System Device And Method For Transfecting Cells For Therapeutic Uses
EP2160459A2 (en) 2007-05-23 2010-03-10 Nature Technology Corp. Improved e. coli plasmid dna production
WO2008151093A1 (en) 2007-05-30 2008-12-11 Alburtylab, Inc. Liquid to liquid biological particle concentrator
JP5532218B2 (ja) 2007-09-10 2014-06-25 日本電気株式会社 試料充填装置
GB0724860D0 (en) 2007-12-20 2008-01-30 Heptares Therapeutics Ltd Screening
EP4328297A2 (en) 2008-01-17 2024-02-28 Inovio Pharmaceuticals, Inc. Variable current density single needle electroporation system and method
EP2279253B1 (en) 2008-04-09 2016-11-16 Maxcyte, Inc. Engineering and delivery of therapeutic compositions of freshly isolated cells
US9845455B2 (en) 2008-05-15 2017-12-19 Ge Healthcare Bio-Sciences Ab Method for cell expansion
DE102008025968B4 (de) 2008-05-30 2014-08-21 Sartorius Stedim Biotech Gmbh Bioreaktor mit Kondensator
DE602008004752D1 (de) 2008-06-19 2011-03-10 Eppendorf Array Tech Sa Streifen für multiparametrische Assays
JP5459903B2 (ja) 2008-09-02 2014-04-02 株式会社半導体エネルギー研究所 アントラセン誘導体、発光素子、発光装置、電子機器、及び照明装置
US20100076057A1 (en) 2008-09-23 2010-03-25 Northwestern University TARGET DNA INTERFERENCE WITH crRNA
WO2010045002A2 (en) 2008-09-26 2010-04-22 Tocagen Inc. Gene therapy vectors and cytosine deaminases
KR101156886B1 (ko) 2008-11-04 2012-06-21 웅진코웨이주식회사 수위감지장치
CN102282255B (zh) 2008-11-19 2014-05-07 阿迈瑞斯公司 多核苷酸装配的组合物和方法
EP2206723A1 (en) 2009-01-12 2010-07-14 Bonas, Ulla Modular DNA-binding domains
EP2216395A1 (en) 2009-02-09 2010-08-11 Lonza Biologics plc. Bioreactor for the cultivation of mammalian cells
WO2010093966A2 (en) 2009-02-12 2010-08-19 Fred Hutchinson Cancer Research Center Generation of a dna nicking enzyme that stimulates site-specific gene conversion from a homing endonuclease
PL2440941T3 (pl) 2009-06-10 2017-10-31 Cynvenio Biosystems Inc Sposoby i urządzenia z przepływem laminarnym
US8677840B2 (en) 2009-06-12 2014-03-25 Innovaprep Llc Surface sampler for bioterrorism particle detection
EP2461819A4 (en) 2009-07-28 2013-07-31 Sangamo Biosciences Inc METHODS AND COMPOSITIONS FOR TREATING TRI-NUCLEOTIDE REPEAT DISORDERS
RU95663U1 (ru) * 2009-07-29 2010-07-10 Федеральное агентство по науке и инновациям (Роснаука) Устройство для культивирования клеток
US9063136B2 (en) 2009-08-02 2015-06-23 Qvella Corporation Cell concentration, capture and lysis devices and methods of use thereof
US8584535B2 (en) 2009-09-17 2013-11-19 Innova Prep LLC Liquid to liquid biological particle concentrator with disposable fluid path
US8584536B2 (en) 2009-09-21 2013-11-19 Innovaprep Llc Devices, systems and methods for elution of particles from flat filters
GB0922434D0 (en) 2009-12-22 2010-02-03 Ucb Pharma Sa antibodies and fragments thereof
WO2011066498A2 (en) 2009-11-28 2011-06-03 Smartflow Technologies, Inc. Portable filtration unit
TR201815882T4 (tr) 2009-12-10 2018-11-21 Univ Iowa State Res Found Inc Tal efektörü aracılı dna modifikasyonu.
DE102010001779A1 (de) 2010-02-10 2011-08-11 Hamilton Bonaduz Ag Kalibrierbare Sensoreinheit für Reaktionsbehälter
US8726744B2 (en) 2010-02-16 2014-05-20 Innovaprep Llc Portable concentrator
DE202010012382U1 (de) 2010-04-14 2010-12-02 Gassmann, Urs Dicht- und Montageband
EP3078753B1 (en) 2010-05-10 2018-04-18 The Regents of The University of California Methods using endoribonuclease compositions
CN102985132A (zh) 2010-06-03 2013-03-20 加利福尼亚大学董事会 电穿孔电极构造和方法
EP2395087A1 (en) 2010-06-11 2011-12-14 Icon Genetics GmbH System and method of modular cloning
EP2749887A3 (en) 2010-07-23 2014-10-01 Beckman Coulter, Inc. System Or Method Of Including Analytical Units
EP2625264B1 (en) 2010-10-08 2022-12-07 Terumo BCT, Inc. Methods and systems of growing and harvesting cells in a hollow fiber bioreactor system with control conditions
US9361427B2 (en) 2011-02-01 2016-06-07 The Regents Of The University Of California Scar-less multi-part DNA assembly design automation
US8667839B2 (en) 2011-02-18 2014-03-11 Tohoku Gakuin Heat conduction-type sensor for calibrating effects of temperature and type of fluid, and thermal flow sensor and thermal barometric sensor using this sensor
WO2012149470A1 (en) 2011-04-27 2012-11-01 Amyris, Inc. Methods for genomic modification
DE102011106162B3 (de) 2011-06-30 2012-09-13 Sartorius Stedim Biotech Gmbh Bioreaktorbehälter und Integritätsprüfungsverfahren für Bioreaktorbehälter
US9382510B2 (en) 2011-08-25 2016-07-05 Jian Chen Methods and devices for electroporation
US8332160B1 (en) 2011-11-17 2012-12-11 Amyris Biotechnologies, Inc. Systems and methods for engineering nucleic acid constructs using scoring techniques
JP6257520B2 (ja) * 2011-12-01 2018-01-10 ニューヨーク ステム セル ファウンデーション インコーポレイテッド 人工多能性幹細胞または分化細胞を作製するための自動化されたシステム
EP2788033B1 (en) * 2011-12-05 2017-05-31 Factor Bioscience Inc. Methods and products for transfecting cells
ITCO20120004A1 (it) 2012-02-07 2013-08-08 Giuseppe Caccia Apparecchio per elettroporazzione dinamica vaginale e anale
WO2013130824A1 (en) 2012-02-29 2013-09-06 Sangamo Biosciences, Inc. Methods and compositions for treating huntington's disease
US9637739B2 (en) 2012-03-20 2017-05-02 Vilnius University RNA-directed DNA cleavage by the Cas9-crRNA complex
US20150072413A1 (en) 2012-03-29 2015-03-12 Arizona Board Of Regents On Behalf University Of Arizona Cell culture apparatus and culture methods using same
JP6343605B2 (ja) 2012-05-25 2018-06-13 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア Ｒｎａ依存性標的ｄｎａ修飾およびｒｎａ依存性転写調節のための方法および組成物
CN104685116A (zh) * 2012-06-25 2015-06-03 Gen9股份有限公司 用于核酸组装和高通量测序的方法
DK3431497T3 (da) 2012-06-27 2022-10-10 Univ Princeton Spaltede inteiner, konjugater og anvendelser deraf
PL3494997T3 (pl) 2012-07-25 2020-04-30 The Broad Institute, Inc. Indukowalne białka wiążące dna i narzędzia perturbacji genomu oraz ich zastosowania
EP2885408B1 (en) * 2012-08-16 2023-01-25 Synthetic Genomics, Inc. Digital to biological converter
CN104540933A (zh) 2012-08-20 2015-04-22 泰尔茂比司特公司 在细胞扩增系统的生物反应器中加载和分配细胞的方法
EP2911736A4 (en) 2012-10-25 2016-06-15 Oncosec Medical Inc electroporation
WO2014065861A1 (en) * 2012-10-26 2014-05-01 The Trustees Of The University Of Pennsylvania Compositions, methods and microfluidics device for telomerase based in vitro diagnostic assays for detecting circulating tumor cells (ctc)
ES2769310T3 (es) 2012-12-06 2020-06-25 Sigma Aldrich Co Llc Modificación y regulación del genoma basada en CRISPR
US8697359B1 (en) 2012-12-12 2014-04-15 The Broad Institute, Inc. CRISPR-Cas systems and methods for altering expression of gene products
USD731634S1 (en) 2012-12-31 2015-06-09 Innovaprep Llc Aerosol filter and extraction cap
US10612043B2 (en) 2013-03-09 2020-04-07 Agilent Technologies, Inc. Methods of in vivo engineering of large sequences using multiple CRISPR/cas selections of recombineering events
AU2013202805B2 (en) * 2013-03-14 2015-07-16 Gen-Probe Incorporated System and method for extending the capabilities of a diagnostic analyzer
US9499855B2 (en) 2013-03-14 2016-11-22 Elwha Llc Compositions, methods, and computer systems related to making and administering modified T cells
US10119134B2 (en) 2013-03-15 2018-11-06 Abvitro Llc Single cell bar-coding for antibody discovery
US9234213B2 (en) 2013-03-15 2016-01-12 System Biosciences, Llc Compositions and methods directed to CRISPR/Cas genomic engineering systems
EP2981617B1 (en) 2013-04-04 2023-07-05 President and Fellows of Harvard College Therapeutic uses of genome editing with crispr/cas systems
AU2014273082B2 (en) 2013-05-29 2018-11-08 Cellectis A method for producing precise DNA cleavage using Cas9 nickase activity
CN105339076B (zh) 2013-06-25 2018-11-23 利乐拉瓦尔集团及财务有限公司 具有卫生悬挂装置的膜过滤设备
IL303973A (en) 2013-07-09 2023-08-01 Harvard College Multiplexed RNA-guided genome engineering
US9029109B2 (en) 2013-08-07 2015-05-12 President And Fellows Of Harvard College Microfluidic vortex-assisted electroporation system and method
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
US10822606B2 (en) 2013-09-27 2020-11-03 The Regents Of The University Of California Optimized small guide RNAs and methods of use
US20150098954A1 (en) 2013-10-08 2015-04-09 Elwha Llc Compositions and Methods Related to CRISPR Targeting
US10767156B2 (en) 2013-10-24 2020-09-08 Yeda Research And Development Co., Ltd. Polynucleotides encoding BREX system polypeptides and methods of using same
CN103555574A (zh) 2013-11-11 2014-02-05 苏州文曲生物微系统有限公司 一种流式电穿孔装置
WO2015073913A1 (en) 2013-11-16 2015-05-21 Terumo Bct, Inc. Expanding cells in a bioreactor
KR102170502B1 (ko) 2013-12-11 2020-10-28 리제너론 파마슈티칼스 인코포레이티드 게놈의 표적화된 변형을 위한 방법 및 조성물
KR20160097338A (ko) 2013-12-12 2016-08-17 더 브로드 인스티튜트, 인코퍼레이티드 뉴클레오티드 반복 장애에서의 ｃｒｉｓｐｒ-ｃａｓ 시스템의 조성물 및 방법 및 용도
DE102013114732A1 (de) 2013-12-20 2015-06-25 Hamilton Bonaduz Ag Abdeckvorrichtung, insbesondere Deckel für die Abdeckung von Reaktionsgefäßen
CN106459898A (zh) 2013-12-20 2017-02-22 哈佛大学校长及研究员协会 低剪切微流控装置及其使用方法和制造方法
KR101598847B1 (ko) 2014-01-23 2016-03-02 부경대학교 산학협력단 액적의 직접 충전 및 전기영동에 기반한 전기천공 기기, 장치 및 방법
US10787654B2 (en) 2014-01-24 2020-09-29 North Carolina State University Methods and compositions for sequence guiding Cas9 targeting
PL3105328T3 (pl) 2014-02-11 2020-10-19 The Regents Of The University Of Colorado, A Body Corporate Umożliwiana przez CRISPR multipleksowa modyfikacja genomu
WO2015143558A1 (en) 2014-03-27 2015-10-01 British Columbia Cancer Agency Branch T-cell epitope identification
EP3126503A1 (en) 2014-04-03 2017-02-08 Massachusetts Institute Of Technology Methods and compositions for the production of guide rna
KR102406585B1 (ko) * 2014-04-14 2022-06-07 맥스시티 인코포레이티드 게놈 dna를 변형하기 위한 방법 및 조성물
CA2947426C (en) 2014-04-29 2020-01-07 Illumina, Inc. Multiplexed single cell gene expression analysis using template switch and tagmentation
WO2015183026A1 (ko) 2014-05-28 2015-12-03 주식회사 툴젠 불활성화된 표적 특이적 뉴클레아제를 이용한 표적 dna의 분리 방법
EP3164482A1 (en) 2014-07-03 2017-05-10 Massachusetts Institute of Technology Microfluidic assay for rapid optimization of cell electroporation
US20160053272A1 (en) 2014-07-18 2016-02-25 Whitehead Institute For Biomedical Research Methods Of Modifying A Sequence Using CRISPR
US20160053304A1 (en) 2014-07-18 2016-02-25 Whitehead Institute For Biomedical Research Methods Of Depleting Target Sequences Using CRISPR
GB201415329D0 (en) * 2014-07-21 2014-10-15 Ge Healthcare Bio Sciences Parallel cell processing method and facility
US10429401B2 (en) 2014-07-21 2019-10-01 Beckman Coulter, Inc. Methods and systems for tube inspection and liquid level detection
AU2015292421A1 (en) 2014-07-25 2017-02-16 Novogy, Inc. Promoters derived from Yarrowia lipolytica and Arxula adeninivorans, and methods of use thereof
US20160076093A1 (en) 2014-08-04 2016-03-17 University Of Washington Multiplex homology-directed repair
CN107002078A (zh) 2014-10-09 2017-08-01 生命技术公司 Crispr寡核苷酸和基因剪辑
CN104263646A (zh) 2014-10-10 2015-01-07 国家开发投资公司 一种微藻光生物反应器用管子
BR112017007923B1 (pt) 2014-10-17 2023-12-12 The Penn State Research Foundation Método para produzir manipulação genética mediada por reações multiplex com rna em uma célula receptora, construção de ácido nucleico,cassete de expressão, vetor, célula receptora e célula geneticamente modificada
KR20160079661A (ko) 2014-12-28 2016-07-06 주식회사 펨토펩 운반체의 사용 없이 세포에 물질을 주입하여 변환시킨 세포
WO2016110453A1 (en) 2015-01-06 2016-07-14 Dsm Ip Assets B.V. A crispr-cas system for a filamentous fungal host cell
CN107429262A (zh) 2015-01-07 2017-12-01 英迪公司 一种用于机械和水动力微流体转染的方法以及用于其的设备
WO2016118780A1 (en) * 2015-01-21 2016-07-28 Fred Hutchinson Cancer Research Center Point-of-care and/or portable platform for gene therapy
WO2016145416A2 (en) 2015-03-11 2016-09-15 The Broad Institute, Inc. Proteomic analysis with nucleic acid identifiers
WO2016145290A1 (en) 2015-03-12 2016-09-15 The Trustees Of The University Of Pennsylvania System, method, and device for high-throughput, automated culturing of genetically modified organisms
SG11201707540QA (en) 2015-03-16 2017-10-30 Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft Method of detecting new immunogenic t cell epitopes and isolating new antigen-specific t cell receptors by means of an mhc cell library
KR20170135966A (ko) 2015-04-13 2017-12-08 맥스시티 인코포레이티드 게놈 dna를 변형시키기 위한 방법 및 조성물
CA3184363C (en) 2015-04-21 2024-01-02 Isolation Bio Inc. High resolution systems, kits, apparatus, and methods for high throughput microbiology applications
US20180023045A1 (en) 2015-04-21 2018-01-25 General Automation Lab Technologies, Inc. High resolution systems, kits, apparatus, and methods using combinatorial media strategies for high throughput microbiology applications
US10677793B2 (en) 2015-04-21 2020-06-09 General Automation Lab Technologies Inc. High resolution systems, kits, apparatus, and methods using lateral flow for high throughput microbiology applications
LU92752B1 (en) 2015-06-24 2016-08-07 Université Du Luxembourg Cell culture apparatus and culture methods using same
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
CN115058395A (zh) 2015-06-25 2022-09-16 美商生物细胞基因治疗有限公司 嵌合抗原受体(car)、组合物及其使用方法
EP3322797B1 (en) 2015-07-13 2023-11-29 Institut Pasteur Improving sequence-specific antimicrobials by blocking dna repair
EP3138920B1 (en) 2015-09-07 2018-06-13 Miltenyi Biotec GmbH Disposable cartridge for electroporation
US11261439B2 (en) * 2015-09-18 2022-03-01 President And Fellows Of Harvard College Methods of making guide RNA
CN113774495A (zh) 2015-09-25 2021-12-10 阿布维特罗有限责任公司 用于对天然配对t细胞受体序列进行t细胞受体靶向鉴别的高通量方法
WO2017075294A1 (en) 2015-10-28 2017-05-04 The Board Institute Inc. Assays for massively combinatorial perturbation profiling and cellular circuit reconstruction
US11092607B2 (en) 2015-10-28 2021-08-17 The Board Institute, Inc. Multiplex analysis of single cell constituents
US11261435B2 (en) 2015-11-05 2022-03-01 Agency For Science, Technology And Research Chemical-inducible genome engineering technology
EP3374494A4 (en) 2015-11-11 2019-05-01 Coda Biotherapeutics, Inc. CRISPR COMPOSITIONS AND METHODS OF USE FOR GENE THERAPY
US9988624B2 (en) 2015-12-07 2018-06-05 Zymergen Inc. Microbial strain improvement by a HTP genomic engineering platform
EP3858996B1 (en) * 2015-12-07 2022-08-03 Zymergen Inc. Microbial strain improvement by a htp genomic engineering platform
US11151497B2 (en) 2016-04-27 2021-10-19 Zymergen Inc. Microbial strain design system and methods for improved large-scale production of engineered nucleotide sequences
WO2017106414A1 (en) 2015-12-18 2017-06-22 Danisco Us Inc. Methods and compositions for polymerase ii (pol-ii) based guide rna expression
US20190017072A1 (en) 2016-01-12 2019-01-17 Sqz Biotechnologies Company Intracellular delivery of complexes
EP3199632A1 (en) 2016-01-26 2017-08-02 ACIB GmbH Temperature-inducible crispr/cas system
US9896696B2 (en) 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
CN109922885B (zh) * 2016-03-16 2022-05-10 伯克利之光生命科技公司 用于基因组编辑克隆的选择和传代的方法、系统和装置
SG11201808014SA (en) 2016-03-18 2018-10-30 Qt Holdings Corp Compositions, devices, and methods for cell separation
US11225638B2 (en) 2016-04-04 2022-01-18 CyteQuest, Inc. System, device and method for electroporation of cells
EP3440194A1 (en) 2016-04-04 2019-02-13 ETH Zurich Mammalian cell line for protein production and library generation
US11499168B2 (en) 2016-04-25 2022-11-15 Universitat Basel Allele editing and applications thereof
CA3022611A1 (en) 2016-05-06 2017-11-09 Juno Therapeutics, Inc. Genetically engineered cells and methods of making the same
US20190136224A1 (en) 2016-05-31 2019-05-09 Massachusetts Institute Of Technology Hydrodynamically Controlled Electric Fields for High Throughput Transformation & High Throughput Parallel Transformation Platform
ES2760477T3 (es) 2016-06-02 2020-05-14 Sigma Aldrich Co Llc Uso de proteínas de unión a ADN programables para potenciar la modificación dirigida del genoma
WO2017207822A1 (en) 2016-06-03 2017-12-07 Lonza Limited Single use bioreactor
US11913081B2 (en) 2016-06-06 2024-02-27 The University Of Chicago Proximity-dependent split RNA polymerases as a versatile biosensor platform
EP3475416A4 (en) 2016-06-22 2020-04-29 Icahn School of Medicine at Mount Sinai VIRAL DELIVERY OF RNA USING SELF-CLeavING RIBOZYMES AND CRISPR-BASED APPLICATIONS
US10017760B2 (en) 2016-06-24 2018-07-10 Inscripta, Inc. Methods for generating barcoded combinatorial libraries
CN109312291A (zh) 2016-06-24 2019-02-05 隆萨有限公司 可变直径生物反应器
CA3031152A1 (en) * 2016-07-21 2018-01-25 Celyad Method and apparatus for automated independent parallel batch-processing of cells
WO2018015419A1 (de) 2016-07-22 2018-01-25 Tecan Trading Ag Pipettenspitze für eine automatisierte pipettiervorrichtung sowie verfahren zu deren herstellung
US20190264193A1 (en) 2016-08-12 2019-08-29 Caribou Biosciences, Inc. Protein engineering methods
DE102016115403A1 (de) 2016-08-19 2018-02-22 Hamilton Bonaduz Ag Autonome Vorrichtung zum Erfassen von Eigenschaften eines Messmediums und Verfahren dafür
EP3516056A1 (en) 2016-09-23 2019-07-31 DSM IP Assets B.V. A guide-rna expression system for a host cell
WO2018071672A1 (en) 2016-10-12 2018-04-19 The Regents Of The University Of Colorado Novel engineered and chimeric nucleases
WO2018073391A1 (en) 2016-10-19 2018-04-26 Cellectis Targeted gene insertion for improved immune cells therapy
FI3535290T3 (fi) 2016-11-07 2024-02-05 Genovie Ab Keinotekoinen kaksiosainen solulaite T-solun reseptorin vuorovaikutuksen havaitsemiseksi ja luonnehtimiseksi kognaattiantigeenin kanssa
US11001796B2 (en) 2016-11-23 2021-05-11 The Charles Stark Draper Laboratory, Inc. Bi-layer multi-well cell culture platform
US20180179485A1 (en) 2016-12-22 2018-06-28 The Charles Stark Draper Laboratory, Inc. System and method of using a microfluidic electroporation device for cell treatment
US20180203017A1 (en) 2016-12-30 2018-07-19 The Board Of Trustees Of The Leland Stanford Junior University Protein-protein interaction detection systems and methods of use thereof
EP3571582A4 (en) * 2017-01-20 2020-12-16 Lifefoundry, Inc. SYSTEMS AND METHODS FOR SUPPORTING MULTIPLE PROCESS AUTOMATION
WO2018152325A1 (en) 2017-02-15 2018-08-23 Bluebird Bio, Inc. Donor repair templates multiplex genome editing
EP3601576A1 (en) 2017-03-24 2020-02-05 CureVac AG Nucleic acids encoding crispr-associated proteins and uses thereof
WO2018191715A2 (en) 2017-04-14 2018-10-18 Synthetic Genomics, Inc. Polypeptides with type v crispr activity and uses thereof
US10011849B1 (en) 2017-06-23 2018-07-03 Inscripta, Inc. Nucleic acid-guided nucleases
US9982279B1 (en) 2017-06-23 2018-05-29 Inscripta, Inc. Nucleic acid-guided nucleases
US20190002874A1 (en) 2017-06-30 2019-01-03 Inscripta, Inc. Cell libraries created using rationally designed nucleic acids
CN114958772A (zh) 2017-09-01 2022-08-30 隆萨沃克斯维尔股份有限公司 端到端细胞治疗自动化
WO2019055878A2 (en) 2017-09-15 2019-03-21 The Board Of Trustees Of The Leland Stanford Junior University MULTIPLEX PRODUCTION AND BAR CODIFICATION OF GENETICALLY MODIFIED CELLS
WO2019075200A1 (en) 2017-10-12 2019-04-18 The Jackson Laboratory METHODS AND COMPOSITIONS OF TRANSGENIC SELECTION
US20190225928A1 (en) 2018-01-22 2019-07-25 Inscripta, Inc. Automated cell processing methods, modules, instruments, and systems comprising filtration devices
WO2019200004A1 (en) 2018-04-13 2019-10-17 Inscripta, Inc. Automated cell processing instruments comprising reagent cartridges
WO2019209926A1 (en) 2018-04-24 2019-10-31 Inscripta, Inc. Automated instrumentation for production of peptide libraries
US10227576B1 (en) 2018-06-13 2019-03-12 Caribou Biosciences, Inc. Engineered cascade components and cascade complexes
CA3108767A1 (en) 2018-06-30 2020-01-02 Inscripta, Inc. Instruments, modules, and methods for improved detection of edited sequences in live cells
AU2019311290A1 (en) 2018-07-26 2021-01-28 Ospedale Pediatrico Bambino Gesù (Opbg) Therapeutic preparations of gamma-delta T cells and Natural Killer cells and methods for manufacture and use
JP2021534798A (ja) 2018-08-28 2021-12-16 フラッグシップ パイオニアリング イノベーションズ シックス，エルエルシー ゲノムを調節するための方法及び組成物
CN112955540A (zh) 2018-08-30 2021-06-11 因思科瑞普特公司 在自动化模块和仪器中对经核酸酶经编辑的序列的改进的检测
GB201816522D0 (en) 2018-10-10 2018-11-28 Autolus Ltd Methods and reagents for analysing nucleic acids from single cells
WO2020191102A1 (en) 2019-03-18 2020-09-24 The Broad Institute, Inc. Type vii crispr proteins and systems
KR20210143230A (ko) 2019-03-19 2021-11-26 더 브로드 인스티튜트, 인코퍼레이티드 뉴클레오티드 서열을 편집하기 위한 방법 및 조성물
GB201905651D0 (en) 2019-04-24 2019-06-05 Lightbio Ltd Nucleic acid constructs and methods for their manufacture
US10837021B1 (en) 2019-06-06 2020-11-17 Inscripta, Inc. Curing for recursive nucleic acid-guided cell editing
US10927385B2 (en) 2019-06-25 2021-02-23 Inscripta, Inc. Increased nucleic-acid guided cell editing in yeast
US10704033B1 (en) 2019-12-13 2020-07-07 Inscripta, Inc. Nucleic acid-guided nucleases
US20210317444A1 (en) 2020-04-08 2021-10-14 Inscripta, Inc. System and method for gene editing cassette design

Also Published As

Publication number Publication date
AU2018291041B2 (en) 2021-08-05
EP3645719A4 (en) 2020-07-15
CA3066253C (en) 2023-10-31
US20190316120A1 (en) 2019-10-17
US20190002814A1 (en) 2019-01-03
US10465185B1 (en) 2019-11-05
KR20220104847A (ko) 2022-07-26
PT3645719T (pt) 2022-05-18
RU2021123421A (ru) 2021-09-10
US11203751B2 (en) 2021-12-21
US10894958B1 (en) 2021-01-19
DK3645719T3 (da) 2022-05-16
US10689645B1 (en) 2020-06-23
US10787663B1 (en) 2020-09-29
US10253316B2 (en) 2019-04-09
US10647982B1 (en) 2020-05-12
PL3645719T3 (pl) 2022-06-20
EP3645719A1 (en) 2020-05-06
NZ760206A (en) 2020-09-25
WO2019006436A1 (en) 2019-01-03
AU2021212069A1 (en) 2021-08-26
EP4270012A2 (en) 2023-11-01
US10954512B1 (en) 2021-03-23
US20210222159A1 (en) 2021-07-22
HUE058668T2 (hu) 2022-09-28
KR20200024247A (ko) 2020-03-06
KR102424850B1 (ko) 2022-07-22
US10584333B1 (en) 2020-03-10
US10329559B1 (en) 2019-06-25
ES2913457T3 (es) 2022-06-02
US20200063123A1 (en) 2020-02-27
US20200270601A1 (en) 2020-08-27
EP3645719B1 (en) 2022-03-09
US20190169605A1 (en) 2019-06-06
US20190270987A1 (en) 2019-09-05
US10947532B2 (en) 2021-03-16
US20210180047A1 (en) 2021-06-17
CA3066253A1 (en) 2019-01-03
EP4270012A3 (en) 2024-04-03
US20190002874A1 (en) 2019-01-03
IL271196B (en) 2020-10-29
CN111094567A (zh) 2020-05-01
US20190185846A1 (en) 2019-06-20
RU2020103731A (ru) 2021-07-30
US20210002634A1 (en) 2021-01-07
US11034953B1 (en) 2021-06-15
MX2019015505A (es) 2020-07-28
HRP20220615T1 (hr) 2022-06-24
US10323242B1 (en) 2019-06-18
US11597921B2 (en) 2023-03-07
US20210284994A1 (en) 2021-09-16
RU2020103731A3 (sr) 2021-07-30
RU2755308C2 (ru) 2021-09-15
US20210108195A1 (en) 2021-04-15
US10584334B1 (en) 2020-03-10
SI3645719T1 (sl) 2022-07-29
US20220195512A1 (en) 2022-06-23
US20200332285A1 (en) 2020-10-22
EP3848459A1 (en) 2021-07-14
US10421959B1 (en) 2019-09-24
US20200080078A1 (en) 2020-03-12
US10519437B1 (en) 2019-12-31
US20200157532A1 (en) 2020-05-21
LT3645719T (lt) 2022-05-25
US10738301B1 (en) 2020-08-11
US20190292536A1 (en) 2019-09-26
AU2018291041A1 (en) 2020-01-16
JP2020530979A (ja) 2020-11-05

Similar Documents

Publication Publication Date Title
IL271196B (en) Methods, modules, devices and systems for mechanized cell processing
ZA202004713B (en) Automated cell processing systems and methods
EP3688137A4 (en) PROCESSES, MODULES, INSTRUMENTS AND SYSTEMS FOR AUTOMATED CELL PROCESSING, AND SYSTEMS INCLUDING CONTINUOUS FLOW ELECTROPORATION DEVICES
IL265469A (en) Interval tracking using sample tracking chains, systems and methods
SG11202001916TA (en) Systems, methods and hydrogels for cell culture and analysis
HK1246941A1 (zh) 訂單處理方法及系統
GB201911162D0 (en) Devices, systems, and methods for sample collection
EP3469338A4 (en) IMAGE-BASED CELL SORTING SYSTEMS AND METHODS
EP3391058A4 (en) SYSTEMS AND METHODS FOR AUTOMATED ANALYSIS
EP3697536A4 (en) DEVICES, SYSTEMS AND METHODS FOR SINGLE-CELL ANALYSIS WITH HIGH THROUGHPUT
HK1246872A1 (zh) 基板處理系統及基板處理方法、以及器件製造方法
EP3573757A4 (en) SYSTEMS AND PROCEDURES FOR WHOLE-CELL ANALYSIS
HK1243177A1 (zh) 平行細胞處理方法和設施
HK1204192A1 (en) Small cell system, related equipment and data processing method
EP3587550A4 (en) CELL PROCESSING SYSTEM AND CELL PROCESSING DEVICE
HK1193630A1 (en) Cell magnetic sorting system, sorting device and processing equipment
EP3246844A4 (en) System processing method, system processing device and terminal
EP3682024A4 (en) METHODS AND SYSTEMS FOR AUTOMATIC SAMPLE PROCESSING
EP3495824A4 (en) ANALYSIS CELL, ANALYSIS DEVICE, ANALYSIS EQUIPMENT AND ANALYSIS SYSTEM
EP3360148A4 (en) MODULES, SYSTEMS AND METHODS FOR MONITORING ENVIRONMENTAL CONDITIONS
GB2559382B (en) Systems and methods for cell range extension
EP3755051A4 (en) METHOD, DEVICE AND SYSTEM FOR CELL PROCESSING
IL254952A0 (en) Monitoring methods and biomass processing systems
EP3383051A4 (en) Information processing device, information processing method, receiving device, and receiving method
GB201607402D0 (en) Systems, devices, and methods for distributed processing