RO81640B - Procedeu de preparare a insulinei - Google Patents

Procedeu de preparare a insulinei

Info

Publication number
RO81640B
RO81640B RO103807A RO10380781A RO81640B RO 81640 B RO81640 B RO 81640B RO 103807 A RO103807 A RO 103807A RO 10380781 A RO10380781 A RO 10380781A RO 81640 B RO81640 B RO 81640B
Authority
RO
Romania
Prior art keywords
insulin
total
chain
dithiothreitol
chains
Prior art date
Application number
RO103807A
Other languages
English (en)
Other versions
RO81640A (ro
Inventor
Ronald Eugene Chance
James Arthur Hoffmann
Original Assignee
Eli Lilly And Company
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eli Lilly And Company filed Critical Eli Lilly And Company
Publication of RO81640A publication Critical patent/RO81640A/ro
Publication of RO81640B publication Critical patent/RO81640B/ro

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/06—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents
    • C07K1/061—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups
    • C07K1/067—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length using protecting groups or activating agents using protecting groups for sulfur-containing functions
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/12—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by hydrolysis, i.e. solvolysis in general

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Endocrinology (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Diabetes (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Procedeul de preparare a insulinei prin combinarea unei catrene A de insulina si o catrena B de insulina pentru producerea unei insuline prin combinarea unei catene A de insulina si o catena B de insulina pentru producerea unei insuline bovine, porcine, umane sau analogi de insulina care consta în faptul ca combinarea celor doua catene se executa într-o singura faza iar procedeul consta în reactionarea formei sulfonate a catenei A de insulina si a formei s-sulfonate a catrenei B de insulina împreuna cu un mediu apos în conditii care sa dea un amestec avînd un pH cuprins între 8 si circa 12 p concentratie totala de proteina cuprinsa între 0,1 si circa 50 mg pe ml si o cantitate de agent reducîtor de tiol care sa determine un total cuprins între circa un total cuprins între circa 0,4 si circa 2,5 grupe sH pentru fiecare grupa -SSO3 prezenta în cantitatea totala a S-sulfonati de catena A si B si permitînd formarea de insulina prin mentinerea amestecului la o temperatura de 0....25 degree C si într-un mediu continînd o sursa de oxigen. Agentul reducator de tiol este ales dintre diotiotreitol de tiol este ales dintre ditiotreitol ditioeritritol si acidul 2-amino-3-mercaptopropionic.
RO103807A 1980-03-27 1981-03-24 Procedeu de preparare a insulinei RO81640B (ro)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US13439080A 1980-03-27 1980-03-27

Publications (2)

Publication Number Publication Date
RO81640A RO81640A (ro) 1983-04-29
RO81640B true RO81640B (ro) 1983-04-30

Family

ID=22463156

Family Applications (1)

Application Number Title Priority Date Filing Date
RO103807A RO81640B (ro) 1980-03-27 1981-03-24 Procedeu de preparare a insulinei

Country Status (28)

Country Link
EP (1) EP0037256B1 (ro)
JP (1) JPS56154443A (ro)
KR (1) KR840000947B1 (ro)
AR (1) AR227305A1 (ro)
AT (1) ATE4638T1 (ro)
AU (1) AU540645B2 (ro)
CA (1) CA1155109A (ro)
CS (1) CS236463B2 (ro)
DD (1) DD157613A5 (ro)
DE (1) DE3160862D1 (ro)
DK (1) DK149756C (ro)
EG (1) EG15248A (ro)
ES (1) ES8207551A1 (ro)
FI (1) FI810918L (ro)
GB (1) GB2072680B (ro)
GR (1) GR73619B (ro)
HU (1) HU185416B (ro)
IE (1) IE51608B1 (ro)
IL (1) IL62483A (ro)
NO (1) NO151897C (ro)
NZ (1) NZ196610A (ro)
PH (1) PH16153A (ro)
PL (1) PL128599B1 (ro)
PT (1) PT72733B (ro)
RO (1) RO81640B (ro)
SU (1) SU1327790A3 (ro)
YU (1) YU76981A (ro)
ZA (1) ZA811970B (ro)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4451396A (en) * 1983-08-01 1984-05-29 Eli Lilly And Company Process for inhibiting undesired thiol reactions during cyanogen bromide cleavage of peptides
DE3501641A1 (de) * 1985-01-19 1986-07-24 Hoechst Ag, 6230 Frankfurt Verfahren zur gewinnung von insulin-vorlaeufern aus reaktionsgemischen, die bei der faltung von insulin-vorlaeufern aus den entsprechenden s-sulfonaten anfallen
US4835251A (en) * 1986-06-23 1989-05-30 Genetech, Inc. Method of chain combination
GB8927546D0 (en) * 1989-12-06 1990-02-07 Ciba Geigy Process for the production of biologically active tgf-beta
JP2606100Y2 (ja) * 1992-04-15 2000-09-11 松下電器産業株式会社 電気掃除機とそのパックフィルター
JP3406244B2 (ja) * 1999-04-30 2003-05-12 伊藤ハム株式会社 新規な融合蛋白質からの組み換えインスリンの製造方法

Also Published As

Publication number Publication date
IL62483A (en) 1984-05-31
DE3160862D1 (en) 1983-10-20
ES500750A0 (es) 1982-09-16
AU540645B2 (en) 1984-11-29
DD157613A5 (de) 1982-11-24
FI810918L (fi) 1981-09-28
EP0037256A1 (en) 1981-10-07
DK149756B (da) 1986-09-22
YU76981A (en) 1983-09-30
KR830004855A (ko) 1983-07-20
NZ196610A (en) 1984-03-16
IE810680L (en) 1981-09-27
EG15248A (en) 1985-12-31
SU1327790A3 (ru) 1987-07-30
DK149756C (da) 1987-06-15
RO81640A (ro) 1983-04-29
EP0037256B1 (en) 1983-09-14
GR73619B (ro) 1984-03-26
PL128599B1 (en) 1984-02-29
DK136581A (da) 1981-09-28
IE51608B1 (en) 1987-01-21
PT72733B (en) 1982-03-22
AR227305A1 (es) 1982-10-15
JPS6318960B2 (ro) 1988-04-20
PH16153A (en) 1983-07-12
ATE4638T1 (de) 1983-09-15
GB2072680A (en) 1981-10-07
AU6884481A (en) 1981-10-01
NO151897C (no) 1985-06-26
NO151897B (no) 1985-03-18
HU185416B (en) 1985-02-28
ES8207551A1 (es) 1982-09-16
ZA811970B (en) 1982-11-24
KR840000947B1 (ko) 1984-07-01
CS236463B2 (en) 1985-05-15
CA1155109A (en) 1983-10-11
JPS56154443A (en) 1981-11-30
PT72733A (en) 1981-04-01
IL62483A0 (en) 1981-05-20
GB2072680B (en) 1983-09-21
NO811038L (no) 1981-09-28
PL230295A1 (ro) 1981-11-13

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