RO68447B - Metoda pentru determinarea simultana sau individuala a izoenzimelor lactatdehidrogenazei - Google Patents
Metoda pentru determinarea simultana sau individuala a izoenzimelor lactatdehidrogenazeiInfo
- Publication number
- RO68447B RO68447B RO83353A RO8335375A RO68447B RO 68447 B RO68447 B RO 68447B RO 83353 A RO83353 A RO 83353A RO 8335375 A RO8335375 A RO 8335375A RO 68447 B RO68447 B RO 68447B
- Authority
- RO
- Romania
- Prior art keywords
- simultaneous
- lactate dehydrogenase
- individual determination
- lactate
- pyruvate
- Prior art date
Links
- 102000003855 L-lactate dehydrogenase Human genes 0.000 title abstract 2
- 108700023483 L-lactate dehydrogenases Proteins 0.000 title abstract 2
- 238000000034 method Methods 0.000 title abstract 2
- 108010044467 Isoenzymes Proteins 0.000 title 1
- JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 abstract 2
- BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical class NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 abstract 2
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 abstract 2
- 210000001124 body fluid Anatomy 0.000 abstract 2
- 239000010839 body fluid Substances 0.000 abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 abstract 2
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 abstract 2
- RXGJTUSBYWCRBK-UHFFFAOYSA-M 5-methylphenazinium methyl sulfate Chemical compound COS([O-])(=O)=O.C1=CC=C2[N+](C)=C(C=CC=C3)C3=NC2=C1 RXGJTUSBYWCRBK-UHFFFAOYSA-M 0.000 abstract 1
- 102000001708 Protein Isoforms Human genes 0.000 abstract 1
- 108010029485 Protein Isoforms Proteins 0.000 abstract 1
- 239000007853 buffer solution Substances 0.000 abstract 1
- 239000007788 liquid Substances 0.000 abstract 1
- 229950006238 nadide Drugs 0.000 abstract 1
- 229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 abstract 1
- 230000009466 transformation Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S604/00—Surgery
- Y10S604/904—Tampons
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
Metoda pentru determinarea simultana sau individuala a izoenzimelor lactatdehidrogenazei prin intermediul transformarii lactatului si a nicotinamid-adenindinucleotidei în piruvat, caracterizat prin aceea ca transformarea se efectueaza într-un mediu lichid într-un sistem tampon trietanolamina-NaOH, si în prezenta a 0,01...1,0 ml fenazinmetosulfat, atît lichidul de corp cercetat cît si reactivul de test folosit se aduc la un pH de 6,3...6,4 fie reactivul de test se aduce la un astfel de pH = 6...6,5 al lichidului de corp analizat, la transformarea lactatului si a nicotinamid-adenin-dinucleotidei în piruvat, sa se asigure un pH de 6...6,5 de preferinta 6,3...6,4.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19742443741 DE2443741C3 (de) | 1974-09-12 | Verfahren zur gemeinsamen Bestimmung der Isoenzyme 4 und 5 der LDH |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RO68447B true RO68447B (ro) | 1983-01-30 |
| RO68447A RO68447A (ro) | 1983-02-01 |
Family
ID=5925603
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RO7583353A RO68447A (ro) | 1974-09-12 | 1975-09-10 | Metoda pentru determinarea simultana sau individuala a izoenzimelor lactatdehidrogenazei |
Country Status (31)
| Country | Link |
|---|---|
| US (2) | US4003795A (ro) |
| JP (2) | JPS5512239B2 (ro) |
| AR (1) | AR211523A1 (ro) |
| AT (1) | AT351173B (ro) |
| AU (1) | AU503460B2 (ro) |
| BE (1) | BE833348A (ro) |
| BR (1) | BR7505850A (ro) |
| CA (1) | CA1076010A (ro) |
| CH (1) | CH631209A5 (ro) |
| CS (1) | CS251752B2 (ro) |
| DD (1) | DD120296A1 (ro) |
| DK (1) | DK144659C (ro) |
| ES (1) | ES440870A1 (ro) |
| FI (1) | FI58943C (ro) |
| FR (1) | FR2284882A1 (ro) |
| GB (1) | GB1527243A (ro) |
| HK (1) | HK79879A (ro) |
| HU (1) | HU171962B (ro) |
| IE (1) | IE41901B1 (ro) |
| IL (1) | IL48071A (ro) |
| IN (1) | IN142896B (ro) |
| IT (1) | IT1155451B (ro) |
| NL (1) | NL178086C (ro) |
| NO (1) | NO144929C (ro) |
| PL (1) | PL98619B1 (ro) |
| RO (1) | RO68447A (ro) |
| SE (1) | SE417109B (ro) |
| SU (2) | SU663329A3 (ro) |
| TR (1) | TR19139A (ro) |
| YU (1) | YU218175A (ro) |
| ZA (1) | ZA755704B (ro) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4311791A (en) * | 1978-06-05 | 1982-01-19 | Bernstein Larry H | Automated kinetic determination of lactate dehydrogenase isoenzymes in serum |
| US4309184A (en) * | 1980-05-08 | 1982-01-05 | Majid Ali | Method of determining food or chemical allergy and intolerance |
| US4543327A (en) * | 1980-06-10 | 1985-09-24 | Bernstein Larry H | Malate dehydrogenase method |
| DE3231287C2 (de) * | 1982-08-23 | 1984-09-06 | MEDI-PHARMA Vertriebsgesellschaft mbH, 5000 Köln | Verfahren zum Herstellen einer diagnostischen Vorrichtung |
| DE3231288C2 (de) * | 1982-08-23 | 1984-06-28 | MEDI-PHARMA Vertriebsgesellschaft mbH, 5000 Köln | Diagnostische Vorrichtung und ihre Verwendung |
| GB2147415B (en) * | 1983-08-05 | 1987-02-11 | Powell & Scholefield Limited | Device and method for antibiotic sensitivity testing |
| DK427784A (da) * | 1984-09-06 | 1986-03-07 | Forenede Bryggerier As | Fremgangsmaade og apparat til bestemmelse af vitalitet i froe |
| US4849347A (en) * | 1984-11-29 | 1989-07-18 | Hoffmann-La Roche Inc. | Colorimetric biological assay |
| US5141853A (en) * | 1988-07-22 | 1992-08-25 | Abbott Laboratories | Determination of ammonia levels in a sample |
| DE69016265T2 (de) * | 1989-06-09 | 1995-09-28 | Abbott Lab | Verfahren zur Bestimmung von Isoenzymen. |
| US5468236A (en) * | 1993-06-09 | 1995-11-21 | Kimberly-Clark Corporation | Disposable absorbent product incorporating chemically reactive substance |
| US7947467B2 (en) * | 2001-07-19 | 2011-05-24 | Common Sense Ltd. | Methods for monitoring pathological conditions in a female subject |
| US20070134740A1 (en) * | 2005-12-12 | 2007-06-14 | David Brusilovsky | Compositions and articles for detection of analytes exceeding a pre-set threshold |
| US7083569B2 (en) * | 2001-08-03 | 2006-08-01 | Zassi Medical Evolutions, Inc. | Ostomy cartridge |
| US20070031914A1 (en) * | 2005-08-05 | 2007-02-08 | Wei Zhu | Devices for analyte assays and methods of use |
| US8053625B2 (en) * | 2006-12-14 | 2011-11-08 | Kimberly-Clark Worldwide, Inc. | Absorbent articles including a body fluid signaling device |
| CN109613177B (zh) | 2014-07-01 | 2021-08-20 | 科蒙森斯公司 | 用于鉴定羊水的诊断组合物 |
| EP3914163A4 (en) * | 2019-01-23 | 2022-04-13 | Gina-Life Diagnostics Ltd. | CANCER DIAGNOSTIC AND MONITORING APPARATUS, SYSTEMS AND METHODS |
| US20220169002A1 (en) | 2019-02-19 | 2022-06-02 | Nitto Denko Corporation | Method for producing laminate of two-dimensional material and laminate |
| US20230054322A1 (en) * | 2021-08-23 | 2023-02-23 | Leslie P. Taylor | Urine indication pad with inbuilt diagnostics for training and indication of potential disease |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2905169A (en) * | 1955-03-10 | 1959-09-22 | Herbert E Nieburgs | Device for cancer detection |
| US2999052A (en) | 1959-03-16 | 1961-09-05 | Miles Lab | Composition for colorimetric test for serum enzymes |
| US3326777A (en) * | 1964-12-10 | 1967-06-20 | Warner Lambert Pharmaceutical | Process for differentiating the isoenzymes of lactic dehydrogenase |
| US3388044A (en) * | 1965-12-07 | 1968-06-11 | Warner Lambert Pharmaceutical | Process for differentiating the isoenzymes of lactic dehydrogenase |
| DE1959410C3 (de) * | 1969-11-26 | 1974-02-28 | Boehringer Mannheim Gmbh, 6800 Mannheim | Indikator zur Bestimmung der reduzierten Pyridincöenzyme |
| US3663374A (en) * | 1970-08-14 | 1972-05-16 | Geomet | Method and apparatus for quantitating enzyme activity |
| DE2061984C3 (de) | 1970-12-16 | 1974-04-11 | Boehringer Mannheim Gmbh, 6800 Mannheim | Reagens zur Bestimmung der Lactat-Dehydrogenase im Farbtest |
| US3862010A (en) * | 1972-06-09 | 1975-01-21 | Technicon Instr | Method of heat fractionating ldh into isoenzyme components |
| US3867258A (en) * | 1973-11-08 | 1975-02-18 | American Cyanamid Co | Lactate dehydrogenase test material |
| US4046634A (en) * | 1974-05-28 | 1977-09-06 | Mercer Donald W | Assay of isoenzymes by ion exchange chromatography |
| US4080263A (en) * | 1976-08-11 | 1978-03-21 | Boehringer Mannheim Gmbh | Process and reagent for the rapid quantitative determination of lactate or alanine |
-
1975
- 1975-08-27 YU YU02181/75A patent/YU218175A/xx unknown
- 1975-08-29 AT AT667075A patent/AT351173B/de not_active IP Right Cessation
- 1975-09-01 NL NLAANVRAGE7510277,A patent/NL178086C/xx not_active IP Right Cessation
- 1975-09-03 GB GB36351/75A patent/GB1527243A/en not_active Expired
- 1975-09-05 ZA ZA00755704A patent/ZA755704B/xx unknown
- 1975-09-09 IT IT69244/75A patent/IT1155451B/it active
- 1975-09-10 DD DD188274A patent/DD120296A1/xx unknown
- 1975-09-10 IL IL48071A patent/IL48071A/xx unknown
- 1975-09-10 RO RO7583353A patent/RO68447A/ro unknown
- 1975-09-11 FI FI752550A patent/FI58943C/fi not_active IP Right Cessation
- 1975-09-11 CH CH1178775A patent/CH631209A5/de not_active IP Right Cessation
- 1975-09-11 SE SE7510130A patent/SE417109B/xx not_active IP Right Cessation
- 1975-09-11 ES ES440870A patent/ES440870A1/es not_active Expired
- 1975-09-11 AU AU84720/75A patent/AU503460B2/en not_active Expired
- 1975-09-11 DK DK406475A patent/DK144659C/da not_active IP Right Cessation
- 1975-09-11 BR BR7505850*A patent/BR7505850A/pt unknown
- 1975-09-11 CA CA235,284A patent/CA1076010A/en not_active Expired
- 1975-09-12 CS CS756214A patent/CS251752B2/cs unknown
- 1975-09-12 PL PL1975183289A patent/PL98619B1/pl unknown
- 1975-09-12 TR TR19139A patent/TR19139A/xx unknown
- 1975-09-12 FR FR7528099A patent/FR2284882A1/fr active Granted
- 1975-09-12 HU HU75SCHA170A patent/HU171962B/hu unknown
- 1975-09-12 BE BE159982A patent/BE833348A/xx not_active IP Right Cessation
- 1975-09-12 US US05/612,633 patent/US4003795A/en not_active Expired - Lifetime
- 1975-09-12 AR AR260373A patent/AR211523A1/es active
- 1975-09-12 IE IE2000/75A patent/IE41901B1/en unknown
- 1975-09-12 JP JP11079975A patent/JPS5512239B2/ja not_active Expired
- 1975-09-12 SU SU752171403A patent/SU663329A3/ru active
- 1975-09-12 NO NO753119A patent/NO144929C/no unknown
- 1975-10-09 IN IN1960/CAL/1975A patent/IN142896B/en unknown
-
1976
- 1976-09-09 SU SU762405873A patent/SU657732A3/ru active
-
1978
- 1978-07-10 US US05/923,641 patent/US4266022A/en not_active Expired - Lifetime
-
1979
- 1979-11-15 HK HK798/79A patent/HK79879A/xx unknown
- 1979-11-22 JP JP54150822A patent/JPS5850720B2/ja not_active Expired
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