GB1318568A - Colourimetric assay of dehydrogenases - Google Patents

Colourimetric assay of dehydrogenases

Info

Publication number
GB1318568A
GB1318568A GB4449870A GB1318568DA GB1318568A GB 1318568 A GB1318568 A GB 1318568A GB 4449870 A GB4449870 A GB 4449870A GB 1318568D A GB1318568D A GB 1318568DA GB 1318568 A GB1318568 A GB 1318568A
Authority
GB
United Kingdom
Prior art keywords
dehydrogenase
composition
phosphate
enzyme
glucose
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB4449870A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Bayer Corp
Original Assignee
Miles Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Miles Laboratories Inc filed Critical Miles Laboratories Inc
Publication of GB1318568A publication Critical patent/GB1318568A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/805—Test papers

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

1318568 Determination of dehydrogenases MILES LABORATORIES Inc 17 Sept 1970 44498/70 Heading B1X A composition for the assay of a dehydrogenase enzyme comprises nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate, a substrate for the enzyme, a terminal acceptor dye and meldola's blue as intermediary hydrogen carrier. The terminal acceptor dye may be 2-(P iodophenyl)-3 (pnitrophenyl)-5-phenyl tetrazolium chloride, bromide or iodide or 2,6-dichloroindophenol. The substrate-enzyme pair may be lactic acid, lactic dehydrogenase; glucose 6-phosphate, glucose-6-phosphate dehydrogenase; 6-phosphogluconate, 6-phosphogluconate dehydrogenase. The composition may be used in aqueous buffered solution or in or on a carrier which may be filter paper thickness 0.01 to 0.2", wood strip, synthetic plastics or woven or non-woven fabric. In use, the composition in solution and the sample are mixed and the rate of change of optical density at 500 mu observed.
GB4449870A 1970-09-17 1970-09-17 Colourimetric assay of dehydrogenases Expired GB1318568A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB4449870 1970-09-17

Publications (1)

Publication Number Publication Date
GB1318568A true GB1318568A (en) 1973-05-31

Family

ID=10433592

Family Applications (1)

Application Number Title Priority Date Filing Date
GB4449870A Expired GB1318568A (en) 1970-09-17 1970-09-17 Colourimetric assay of dehydrogenases

Country Status (3)

Country Link
US (1) US3732147A (en)
CA (1) CA955160A (en)
GB (1) GB1318568A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2147415A (en) * 1983-08-05 1985-05-09 Powell & Scholefield Limited Device and method for antibiotic sensitivity testing
EP0244825A1 (en) * 1986-05-09 1987-11-11 Fuji Photo Film Co., Ltd. Dry-type analytical element for cholesterol
FR2618905A1 (en) * 1987-07-27 1989-02-03 Suekami Pharmaceuti Research METHOD FOR DETERMINING THE PRESENCE OF LIVE SPERMATOZOIDS AND TESTING THEREFOR
US6051393A (en) * 1994-03-29 2000-04-18 Jones; Sonja K. Method of detecting malignant and pre-malignant conditions of the cervix, and test kits therefor

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3852034A (en) * 1972-06-21 1974-12-03 American Sterilizer Co Indicator for effectiveness of ethylene oxide sterilization
US4056485A (en) * 1974-10-04 1977-11-01 Warner-Lambert Company Stable colored reference standard for enzymatic determinations
US4006061A (en) * 1975-12-29 1977-02-01 Monsanto Company Lactate dehydrogenase determination method
US4223799A (en) * 1979-06-11 1980-09-23 Time Saving Falls, Inc. Releasable locking means for closure caps
DE3048662A1 (en) * 1980-12-23 1982-07-22 Boehringer Mannheim Gmbh, 6800 Mannheim STABILIZED PREPARATION OF TETRAZOLIUM SALTS
DE3247894A1 (en) * 1982-12-24 1984-06-28 Merck Patent Gmbh, 6100 Darmstadt TEST SYSTEM AND METHOD FOR DETERMINING NAD (P) H
US4728608A (en) * 1985-04-08 1988-03-01 Cambridge Bioscience Corporation Bacterial screening system with enhanced shelf life
WO1994001578A1 (en) * 1992-07-02 1994-01-20 Boehringer Mannheim Corporation Stabilizing tetrazolium salts in a reagent
DE10001529A1 (en) * 2000-01-15 2001-07-19 Roche Diagnostics Gmbh Stabilized coenzyme solutions and their use for the determination of dehydrogenases or their substrates
TWI275795B (en) * 2001-02-14 2007-03-11 Sysmex Corp Novel assay method
TW201441620A (en) * 2013-04-19 2014-11-01 Nat Univ Tsing Hua Xylem fiber device for detection
TWI490475B (en) * 2013-06-25 2015-07-01 Nat Univ Tsing Hua Three dimensional lignocellulosic detection device
CN105527279A (en) * 2014-09-30 2016-04-27 郑兆珉 Food safety detection apparatus and production method thereof
TWI561819B (en) * 2014-10-31 2016-12-11 Univ Nat Tsing Hua Three dimensional detection device and manufacturing method for the same

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2147415A (en) * 1983-08-05 1985-05-09 Powell & Scholefield Limited Device and method for antibiotic sensitivity testing
EP0244825A1 (en) * 1986-05-09 1987-11-11 Fuji Photo Film Co., Ltd. Dry-type analytical element for cholesterol
FR2618905A1 (en) * 1987-07-27 1989-02-03 Suekami Pharmaceuti Research METHOD FOR DETERMINING THE PRESENCE OF LIVE SPERMATOZOIDS AND TESTING THEREFOR
EP0302765A1 (en) * 1987-07-27 1989-02-08 Clonatec Method for detecting living spermatozoa, and tests therefor
US6051393A (en) * 1994-03-29 2000-04-18 Jones; Sonja K. Method of detecting malignant and pre-malignant conditions of the cervix, and test kits therefor

Also Published As

Publication number Publication date
CA955160A (en) 1974-09-24
US3732147A (en) 1973-05-08

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Legal Events

Date Code Title Description
PS Patent sealed [section 19, patents act 1949]
PLNP Patent lapsed through nonpayment of renewal fees