GB1468849A - Method for determining glutamate and glutamic transminases - Google Patents

Method for determining glutamate and glutamic transminases

Info

Publication number
GB1468849A
GB1468849A GB3059774A GB3059774A GB1468849A GB 1468849 A GB1468849 A GB 1468849A GB 3059774 A GB3059774 A GB 3059774A GB 3059774 A GB3059774 A GB 3059774A GB 1468849 A GB1468849 A GB 1468849A
Authority
GB
United Kingdom
Prior art keywords
glutamic
solution
transaminases
glutamate
july
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB3059774A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Dow Chemical Co
Original Assignee
Dow Chemical Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dow Chemical Co filed Critical Dow Chemical Co
Publication of GB1468849A publication Critical patent/GB1468849A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
    • C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)

Abstract

1468849 Determining glutamate and glutamic transaminases DOW CHEMICAL CO 10 July 1974 [19 July 1973] 30597/74 Heading G1B A method for the determination of glutamate or glutamic transaminases comprises mixing a specimen to be analyzed with a solution of oxidized nicotinamide adenine dinucleotide, glutomate dehydrogenase, a tetrazolium dye and diaphorase or N-methyl phenazonium metho-sulphate, the solution additionally containing a transaminose substrate when glutamic transaminases are to be determined, incubating the mixture under conditions of pH and temperature conducive to the formation of a measurable colour and measuring the colour. The solution used is essentially free of ammonium ions. A buffer may be incorporated in the solution to provide a pH of 7À9 to 8À3. The tetrazolium dye is preferably 2-p-iodophenyl-3-nitrophenyl-5- phenyltetrazolium chloride. The method is particularly applicable to the analyses of biological fluids.
GB3059774A 1973-07-19 1974-07-10 Method for determining glutamate and glutamic transminases Expired GB1468849A (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US38081073A 1973-07-19 1973-07-19

Publications (1)

Publication Number Publication Date
GB1468849A true GB1468849A (en) 1977-03-30

Family

ID=23502536

Family Applications (1)

Application Number Title Priority Date Filing Date
GB3059774A Expired GB1468849A (en) 1973-07-19 1974-07-10 Method for determining glutamate and glutamic transminases

Country Status (8)

Country Link
JP (1) JPS584558B2 (en)
AU (1) AU7031774A (en)
BR (1) BR7405980D0 (en)
CA (1) CA1024870A (en)
DE (1) DE2431779A1 (en)
FR (1) FR2245949B1 (en)
GB (1) GB1468849A (en)
IT (1) IT1032063B (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2834706A1 (en) * 1978-08-08 1980-02-14 Boehringer Mannheim Gmbh METHOD AND REAGENT FOR DETERMINING GLUTAMATE-OXALACETATE TRANSAMINASE AND GLUTAMATE-PYRUVATE TRANSAMINASE
JP2501801B2 (en) * 1986-10-07 1996-05-29 ユニチカ株式会社 Reagent for quantifying γ-glutamyl transpeptidase

Also Published As

Publication number Publication date
DE2431779A1 (en) 1975-02-06
JPS584558B2 (en) 1983-01-26
IT1032063B (en) 1979-05-30
BR7405980D0 (en) 1975-05-13
CA1024870A (en) 1978-01-24
FR2245949A1 (en) 1975-04-25
AU7031774A (en) 1976-01-08
JPS5044894A (en) 1975-04-22
FR2245949B1 (en) 1984-09-28

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee