GB1468849A - Method for determining glutamate and glutamic transminases - Google Patents
Method for determining glutamate and glutamic transminasesInfo
- Publication number
- GB1468849A GB1468849A GB3059774A GB3059774A GB1468849A GB 1468849 A GB1468849 A GB 1468849A GB 3059774 A GB3059774 A GB 3059774A GB 3059774 A GB3059774 A GB 3059774A GB 1468849 A GB1468849 A GB 1468849A
- Authority
- GB
- United Kingdom
- Prior art keywords
- glutamic
- solution
- transaminases
- glutamate
- july
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/52—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving transaminase
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
1468849 Determining glutamate and glutamic transaminases DOW CHEMICAL CO 10 July 1974 [19 July 1973] 30597/74 Heading G1B A method for the determination of glutamate or glutamic transaminases comprises mixing a specimen to be analyzed with a solution of oxidized nicotinamide adenine dinucleotide, glutomate dehydrogenase, a tetrazolium dye and diaphorase or N-methyl phenazonium metho-sulphate, the solution additionally containing a transaminose substrate when glutamic transaminases are to be determined, incubating the mixture under conditions of pH and temperature conducive to the formation of a measurable colour and measuring the colour. The solution used is essentially free of ammonium ions. A buffer may be incorporated in the solution to provide a pH of 7À9 to 8À3. The tetrazolium dye is preferably 2-p-iodophenyl-3-nitrophenyl-5- phenyltetrazolium chloride. The method is particularly applicable to the analyses of biological fluids.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US38081073A | 1973-07-19 | 1973-07-19 |
Publications (1)
Publication Number | Publication Date |
---|---|
GB1468849A true GB1468849A (en) | 1977-03-30 |
Family
ID=23502536
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
GB3059774A Expired GB1468849A (en) | 1973-07-19 | 1974-07-10 | Method for determining glutamate and glutamic transminases |
Country Status (8)
Country | Link |
---|---|
JP (1) | JPS584558B2 (en) |
AU (1) | AU7031774A (en) |
BR (1) | BR7405980D0 (en) |
CA (1) | CA1024870A (en) |
DE (1) | DE2431779A1 (en) |
FR (1) | FR2245949B1 (en) |
GB (1) | GB1468849A (en) |
IT (1) | IT1032063B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE2834706A1 (en) * | 1978-08-08 | 1980-02-14 | Boehringer Mannheim Gmbh | METHOD AND REAGENT FOR DETERMINING GLUTAMATE-OXALACETATE TRANSAMINASE AND GLUTAMATE-PYRUVATE TRANSAMINASE |
JP2501801B2 (en) * | 1986-10-07 | 1996-05-29 | ユニチカ株式会社 | Reagent for quantifying γ-glutamyl transpeptidase |
-
1974
- 1974-06-17 CA CA202,550A patent/CA1024870A/en not_active Expired
- 1974-06-20 AU AU70317/74A patent/AU7031774A/en not_active Expired
- 1974-07-02 DE DE19742431779 patent/DE2431779A1/en not_active Ceased
- 1974-07-10 GB GB3059774A patent/GB1468849A/en not_active Expired
- 1974-07-15 IT IT5211074A patent/IT1032063B/en active
- 1974-07-18 FR FR7425015A patent/FR2245949B1/fr not_active Expired
- 1974-07-18 JP JP49082684A patent/JPS584558B2/en not_active Expired
- 1974-07-19 BR BR598074A patent/BR7405980D0/en unknown
Also Published As
Publication number | Publication date |
---|---|
JPS584558B2 (en) | 1983-01-26 |
DE2431779A1 (en) | 1975-02-06 |
FR2245949B1 (en) | 1984-09-28 |
IT1032063B (en) | 1979-05-30 |
JPS5044894A (en) | 1975-04-22 |
AU7031774A (en) | 1976-01-08 |
FR2245949A1 (en) | 1975-04-25 |
CA1024870A (en) | 1978-01-24 |
BR7405980D0 (en) | 1975-05-13 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
PS | Patent sealed | ||
PCNP | Patent ceased through non-payment of renewal fee |