DE3852825D1 - Schnellverfahren zum nachweis von coliformen mikroorganismen. - Google Patents

Schnellverfahren zum nachweis von coliformen mikroorganismen.

Info

Publication number
DE3852825D1
DE3852825D1 DE3852825T DE3852825T DE3852825D1 DE 3852825 D1 DE3852825 D1 DE 3852825D1 DE 3852825 T DE3852825 T DE 3852825T DE 3852825 T DE3852825 T DE 3852825T DE 3852825 D1 DE3852825 D1 DE 3852825D1
Authority
DE
Germany
Prior art keywords
microorganisms
methylumbelliferone
substrate
detecting
quick procedure
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE3852825T
Other languages
English (en)
Other versions
DE3852825T2 (de
Inventor
James D Berg
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of DE3852825D1 publication Critical patent/DE3852825D1/de
Publication of DE3852825T2 publication Critical patent/DE3852825T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/10—Enterobacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • C12Q1/06—Quantitative determination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/24—Methods of sampling, or inoculating or spreading a sample; Methods of physically isolating an intact microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2334/00—O-linked chromogens for determinations of hydrolase enzymes, e.g. glycosidases, phosphatases, esterases
    • C12Q2334/20—Coumarin derivatives
    • C12Q2334/22—4-Methylumbelliferyl, i.e. beta-methylumbelliferone, 4MU
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/24—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • G01N2333/245—Escherichia (G)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/848—Escherichia
    • Y10S435/849—Escherichia coli

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Toxicology (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Optical Radar Systems And Details Thereof (AREA)
DE3852825T 1987-11-05 1988-11-03 Schnellverfahren zum nachweis von coliformen mikroorganismen. Expired - Fee Related DE3852825T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11748187A 1987-11-05 1987-11-05
PCT/NO1988/000083 WO1989004372A1 (en) 1987-11-05 1988-11-03 Rapid process for detecting pathogenic microorganisms

Publications (2)

Publication Number Publication Date
DE3852825D1 true DE3852825D1 (de) 1995-03-02
DE3852825T2 DE3852825T2 (de) 1995-05-18

Family

ID=22373184

Family Applications (2)

Application Number Title Priority Date Filing Date
DE3852825T Expired - Fee Related DE3852825T2 (de) 1987-11-05 1988-11-03 Schnellverfahren zum nachweis von coliformen mikroorganismen.
DE3855762T Expired - Fee Related DE3855762T2 (de) 1987-11-05 1988-11-03 Direkte Methode zum Nachweis von sehr niedrigerem Grad der Coliform-Kontamination

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE3855762T Expired - Fee Related DE3855762T2 (de) 1987-11-05 1988-11-03 Direkte Methode zum Nachweis von sehr niedrigerem Grad der Coliform-Kontamination

Country Status (6)

Country Link
US (1) US5292644A (de)
EP (2) EP0386051B1 (de)
AT (2) ATE117378T1 (de)
AU (1) AU2602488A (de)
DE (2) DE3852825T2 (de)
WO (1) WO1989004372A1 (de)

Families Citing this family (48)

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DE3811097A1 (de) * 1988-03-31 1989-10-12 Orpegen Med Molekularbioforsch Verfahren zur steuerung biologischer klaerstufen
US5429933A (en) 1986-06-30 1995-07-04 Edberg; Stephen C. Detection of first generation environmental sourced microbes in an environmentally-derived sample
IL85948A0 (en) * 1987-04-08 1988-09-30 Cambridge Bioscience Corp Method and kit for detecting bacteria or fungi
US5518894A (en) * 1987-11-05 1996-05-21 Berg; James D. Rapid coliform detection system
AU616957B2 (en) * 1988-06-20 1991-11-14 Becton Dickinson & Company Device for enhancing fluorescence and kinetics and methods of using the device
US6306621B1 (en) * 1991-11-18 2001-10-23 The United States Of America As Represented By The Administrator Of The U.S. Environmental Protection Agency Membrane filter agar medium for simultaneous detection of total coliforms and E. coli
US5849515A (en) * 1994-04-01 1998-12-15 Hach Company Method and medium for use in detecting E. coli and total coliforms
US5650290A (en) * 1994-04-01 1997-07-22 Hach Company Method & Medium for use in detecting E. coli and total coliforms
US5601998A (en) * 1994-08-18 1997-02-11 Minnesota Mining & Mfg Culture medium and device for detection and enumeration of enterobacteriaceae
ES2298457T3 (es) * 1994-11-04 2008-05-16 Idexx Laboratories, Inc. Medio para detectar microbios objetivo en una muestra.
US5610029A (en) * 1994-11-04 1997-03-11 Idexx Laboratories, Inc. Medium for detecting target microbes in a sample
US5985594A (en) 1995-11-14 1999-11-16 Idexx Laboratories, Inc. Method for quantification of biological material in a sample
US5700655A (en) * 1995-11-14 1997-12-23 Idexx Laboratories, Inc. Method for quantification of biological material in a sample
US7122338B2 (en) * 1995-11-14 2006-10-17 Biocontrol Systems, Inc. Method for quantification of biological material in a sample
IT1284658B1 (it) * 1996-06-03 1998-05-21 Isrim S C A R L Stimolatore dell'attivita' enzimatica in cellule batteriche
IT1284178B1 (it) * 1996-06-27 1998-05-08 Isrim S C A R L Procedimento per la rapida rilevazione in un campione della presenza di cellule batteriche anche se in numero limitatissimo.
US5843699A (en) * 1997-04-08 1998-12-01 Difco Laboratories, Inc. Rapid microorganism detection method
FR2769092B1 (fr) * 1997-09-29 1999-11-12 Lyonnaise Eaux Eclairage Procede de regulation de la desinfection de liquides
WO1999019510A1 (en) 1997-10-10 1999-04-22 President And Fellows Of Harvard College Surface-bound, double-stranded dna protein arrays
US5968762A (en) * 1998-03-19 1999-10-19 The University Of Connecticut Method for detecting bacteria in a sample
US6511819B2 (en) 1998-08-28 2003-01-28 Nye Colifast As Rapid coliform detection system
US5972641A (en) * 1998-08-28 1999-10-26 Colifast Systems Asa Rapid coliform detection system
DE19841588A1 (de) * 1998-09-11 2000-03-23 Nitzsche Frank Verfahren zum Nachweis von Zellen in einer Probe
US6723505B1 (en) 1999-08-13 2004-04-20 Nye Colifast As Method for identification of the indicators of contamination in liquid samples
ES2329986T3 (es) 2001-09-06 2009-12-03 Rapid Micro Biosystems Inc Deteccion rapida de celulas en replicacion.
FR2829500B1 (fr) 2001-09-13 2003-12-12 Hemosystem Procede de concentration et de detection de germes pathogenes a partir de produits sanguins et/ou de leurs derives et dispositif pour le mettre en oeuvre
JP3973876B2 (ja) * 2001-10-30 2007-09-12 株式会社三菱化学ヤトロン 新規の細菌分析方法
US20030138906A1 (en) * 2001-11-05 2003-07-24 Ingun Tryland Fluorescence test for measuring heterotrophic bacteria in water
US20030143658A1 (en) * 2002-01-28 2003-07-31 Casella Linda J. Richardson Rapid methods and devices for the detection of coliform and the detection and confirmation of E. coil
FR2845097B1 (fr) * 2002-10-01 2006-06-16 Metis Biotechnologies Procede de detection et de comptage de microorganismes dans un echantillon
CA2504163C (en) * 2002-10-17 2012-04-03 Conestoga-Rovers & Associates Limited Rapid coliform detection system
ITTR20030002A1 (it) * 2003-07-31 2005-02-01 Isrim Societa Consortile A Respon Sabilita Limit Metodo per la rilevazione di coliformi ed in particolare
ATE555213T2 (de) 2004-03-01 2012-05-15 Mycometer As Kontaminationsmessung
DE102004011822A1 (de) * 2004-03-11 2005-09-29 Cognis Deutschland Gmbh & Co. Kg Qualitätssicherungssystem zum Nachweis von Mikroorganismen
US8373861B2 (en) * 2004-05-11 2013-02-12 Les Entreprises Biospec Global Solutions Inc. System for rapid analysis of microbiological materials in liquid samples
EP2647723B1 (de) 2004-06-23 2017-02-01 Zyzeba Testing Limited Verbesserung an und im Zusammenhang mit einer Mikroorganismusprüfvorrichtung und Verfahren zu deren Verwendung
FR2886651B1 (fr) 2005-06-03 2007-09-21 Anjou Rech Procede de quantification rapide des micro-organismes coliformes vivants dans un echantillon d'eau
US9057046B2 (en) 2005-09-26 2015-06-16 Rapid Micro Biosystems, Inc. Cassette containing growth medium
CN102224410B (zh) 2008-09-24 2017-02-08 施特劳斯控股公司 用于测试分析物的成像分析仪
US20100112630A1 (en) * 2008-11-03 2010-05-06 Scott Martell Boyette Methods for measuring microbiological content in aqueous media
US8481302B2 (en) * 2008-11-03 2013-07-09 General Electric Company Total bacteria monitoring system
FR2980577B1 (fr) * 2011-09-26 2013-09-20 Biomerieux Sa Systeme de detection et/ou de quantification in vitro par fluorimetrie
EP2776550B1 (de) 2011-11-07 2018-01-10 Rapid Micro Biosystems, Inc. Kassette für sterilitätstests
CA3171698A1 (en) 2012-04-16 2013-10-24 Rapid Micro Biosystems, Inc. Cell culturing device
DE102013225037B4 (de) * 2013-12-05 2016-08-25 Asklepios Kliniken Verwaltungsgesellschaft mbH Verfahren zur Erkennung resistenter Keime und Vorrichtung zum Durchführen desselben
WO2019142187A1 (en) 2018-01-17 2019-07-25 Bactobyte Ltd. Methods and compositions for improving detection of microorganisms
WO2019204784A1 (en) 2018-04-19 2019-10-24 First Light Biosciences, Inc. Detection of targets
CN118048434B (zh) * 2024-04-10 2024-07-16 北京挑战生物技术有限公司 一种快速评估抗菌组分对大肠杆菌抑制能力的方法及其应用

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FR2457323A1 (fr) * 1979-05-25 1980-12-19 Api Labor Test permettant la mise en evidence des bacteries du genre salmonella et serratia et leur distinction des genres proteus et providencia
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JPH0611237B2 (ja) * 1982-04-14 1994-02-16 ラジオメーター コーポレイト ディベロップメント リミテッド 微生物学的試験方法
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FR2558847B1 (fr) * 1984-01-31 1986-06-20 Millipore Sa Dispositif et procede de controle microbiologique de liquides
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US5210022A (en) * 1990-04-20 1993-05-11 Rcr Scientific, Inc. Method test media and chromogenic compounds for identifying and differentiating general coliforms and Escherichia coli bacteria

Also Published As

Publication number Publication date
WO1989004372A1 (en) 1989-05-18
DE3855762T2 (de) 1997-05-07
DE3855762D1 (de) 1997-02-27
AU2602488A (en) 1989-06-01
EP0574977B1 (de) 1997-01-15
EP0574977A1 (de) 1993-12-22
EP0386051B1 (de) 1995-01-18
ATE117378T1 (de) 1995-02-15
DE3852825T2 (de) 1995-05-18
US5292644A (en) 1994-03-08
ATE147790T1 (de) 1997-02-15
EP0386051A1 (de) 1990-09-12

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Legal Events

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8339 Ceased/non-payment of the annual fee