PT2951309T - Expressao e processamento de transgene intensificado - Google Patents
Expressao e processamento de transgene intensificadoInfo
- Publication number
- PT2951309T PT2951309T PT14708935T PT14708935T PT2951309T PT 2951309 T PT2951309 T PT 2951309T PT 14708935 T PT14708935 T PT 14708935T PT 14708935 T PT14708935 T PT 14708935T PT 2951309 T PT2951309 T PT 2951309T
- Authority
- PT
- Portugal
- Prior art keywords
- intensified
- transgene
- expression
- processing
- intensified transgene
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
- C07K16/241—Tumor Necrosis Factors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/32—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from animals or humans against translation products of oncogenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/106—Plasmid DNA for vertebrates
- C12N2800/107—Plasmid DNA for vertebrates for mammalian
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/90—Vectors containing a transposable element
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Oncology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361759802P | 2013-02-01 | 2013-02-01 | |
| US201361800244P | 2013-03-15 | 2013-03-15 | |
| US201361806634P | 2013-03-29 | 2013-03-29 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PT2951309T true PT2951309T (pt) | 2019-04-03 |
Family
ID=50239688
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT14708935T PT2951309T (pt) | 2013-02-01 | 2014-02-01 | Expressao e processamento de transgene intensificado |
| PT191533769T PT3536797T (pt) | 2013-02-01 | 2014-02-01 | Expressão e processamento reforçado do transgene |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PT191533769T PT3536797T (pt) | 2013-02-01 | 2014-02-01 | Expressão e processamento reforçado do transgene |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US11898154B2 (enExample) |
| EP (2) | EP2951309B1 (enExample) |
| JP (1) | JP6563816B2 (enExample) |
| KR (1) | KR102235603B1 (enExample) |
| CN (2) | CN120718957A (enExample) |
| AU (2) | AU2014210856B2 (enExample) |
| CA (1) | CA2898878C (enExample) |
| DK (2) | DK2951309T3 (enExample) |
| ES (2) | ES2717175T3 (enExample) |
| FI (1) | FI3536797T3 (enExample) |
| HU (2) | HUE061494T2 (enExample) |
| IL (1) | IL240198B (enExample) |
| LT (1) | LT2951309T (enExample) |
| PL (2) | PL3536797T3 (enExample) |
| PT (2) | PT2951309T (enExample) |
| RU (1) | RU2711505C9 (enExample) |
| SG (2) | SG10201803758XA (enExample) |
| SI (1) | SI2951309T1 (enExample) |
| WO (1) | WO2014118619A2 (enExample) |
| ZA (1) | ZA201506419B (enExample) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109576269B (zh) * | 2014-04-01 | 2020-08-28 | 三生国健药业（上海）股份有限公司 | 一种含mar核心片段的动物细胞表达载体 |
| CN105154473B (zh) * | 2015-09-30 | 2019-03-01 | 上海细胞治疗研究院 | 一种高效安全的转座子整合系统及其用途 |
| US20180265894A1 (en) * | 2015-10-06 | 2018-09-20 | National University Corporation Okayama University | Gene Expression Cassette And Product Thereof |
| JP6871679B2 (ja) * | 2016-03-23 | 2021-05-12 | 国立大学法人 岡山大学 | 遺伝子発現用カセット及びその産生物 |
| JP2017070224A (ja) * | 2015-10-06 | 2017-04-13 | 国立大学法人 岡山大学 | 遺伝子発現用カセット及びその産生物 |
| WO2017109177A1 (en) | 2015-12-24 | 2017-06-29 | Selexis S.A. | Improved eukaryotic cells for protein manufacturing and methods of making them |
| ITUA20161610A1 (it) * | 2016-03-14 | 2017-09-14 | Angela Anna Messina | Composto peptidico farmacologicamente attivo, procedimento per la sua preparazione e suo uso. |
| EP3219800A1 (en) * | 2016-03-15 | 2017-09-20 | Max-Delbrück-Centrum Für Molekulare Medizin | A transposon-based transfection system for primary cells |
| EP3219803A1 (en) * | 2016-03-15 | 2017-09-20 | Max-Delbrück-Centrum für Molekulare Medizin | Enhanced sleeping beauty transposons, kits and methods of transposition |
| WO2017191165A1 (en) * | 2016-05-03 | 2017-11-09 | Lonza Ltd | Modulation of lipid metabolism for protein production |
| JP7382138B2 (ja) * | 2016-10-03 | 2023-11-16 | 第一三共株式会社 | Ｈｓｐａ５遺伝子のプロモーター |
| CA3047313A1 (en) | 2016-12-16 | 2018-06-21 | B-Mogen Biotechnologies, Inc. | Enhanced hat family transposon-mediated gene transfer and associated compositions, systems, and methods |
| US11278570B2 (en) | 2016-12-16 | 2022-03-22 | B-Mogen Biotechnologies, Inc. | Enhanced hAT family transposon-mediated gene transfer and associated compositions, systems, and methods |
| WO2018224162A1 (en) * | 2017-06-09 | 2018-12-13 | Biontech Rna Pharmaceuticals Gmbh | Methods for characterizing loss of antigen presentation |
| JP7240749B2 (ja) * | 2017-09-29 | 2023-03-16 | ベスファジェン・インコーポレイテッド | 心不全の予防または治療のための医薬組成物 |
| CN109929839B (zh) * | 2017-12-18 | 2021-02-12 | 华东师范大学 | 拆分型单碱基基因编辑系统及其应用 |
| CN108504692B (zh) * | 2018-03-26 | 2021-07-23 | 安徽大学 | 一种基因敲除cho细胞株的构建方法及其在治疗性重组蛋白表达中的应用 |
| WO2019219947A1 (en) * | 2018-05-18 | 2019-11-21 | Sorbonne Universite | Molecular tools and methods for transgene integration and their transposition dependent expression |
| WO2019246486A2 (en) | 2018-06-21 | 2019-12-26 | B-Mogen Biotechnologies, Inc. | ENHANCED hAT FAMILY TRANSPOSON-MEDIATED GENE TRANSFER AND ASSOCIATED COMPOSITIONS, SYSTEMS, AND METHODS |
| RU2686102C1 (ru) * | 2018-08-07 | 2019-04-24 | Федеральное Государственное Бюджетное Учреждение Науки Институт Молекулярной Биологии Им. В.А. Энгельгардта Российской Академии Наук (Имб Ран) | Рекомбинантный вектор для создания плазмидных генетических конструкций, обладающих повышенной длительностью экспрессии целевых генов |
| US20210343368A1 (en) * | 2018-09-05 | 2021-11-04 | Max-Delbrück-Centrum Für Molekulare Medizin In Der Helmholtz-Gemeinschaft | Method for Engineering Synthetic Cis-Regulatory DNA |
| WO2020081537A1 (en) * | 2018-10-16 | 2020-04-23 | Texas Tech University System | Method to express, purify, and biotinylate eukaryotic cell-derived major histocompatibility complexes |
| US20230193341A1 (en) | 2018-10-24 | 2023-06-22 | Selexis Sa | Expression systems, recombinant cells and uses thereof |
| HUE068630T2 (hu) | 2018-12-10 | 2025-01-28 | Amgen Inc | Mutáns PiggyBac transzpozáz |
| US12404527B2 (en) * | 2018-12-24 | 2025-09-02 | Selexs S.A. | Characterization and inactivation of endogenous retroviruses in chinese hamster ovary cells |
| US20220088158A1 (en) | 2019-01-23 | 2022-03-24 | Aceragen, Inc. | Method of ameliorating a pro-inflammatory immunophenotype in farber disease subjects by repeated administration of a recombinant human acid ceramidase |
| IT201900002321A1 (it) * | 2019-02-18 | 2020-08-18 | Univ Degli Studi Padova | Peptidi con attivita' antitumorale |
| AU2020248453A1 (en) * | 2019-03-27 | 2021-10-28 | Phoenix Tissue Repair, Inc. | Systems and methods for producing collagen 7 compositions |
| CN110006866B (zh) * | 2019-04-16 | 2022-04-22 | 杭州迈尔德生物科技有限公司 | 一种阿片类活性物质的通用检测方法及其检测试剂盒 |
| WO2020228721A1 (en) * | 2019-05-13 | 2020-11-19 | Lin Mei Chun | Method and kit for monitoring cancer |
| EP4041895A1 (en) * | 2019-10-10 | 2022-08-17 | The Regents of the University of California | Methods to stabilize mammalian cells |
| IL270306A (en) | 2019-10-30 | 2021-05-31 | Yeda Res & Dev | Prevention and treatment of pre-myeloid and myeloid malignancies |
| CN112813099A (zh) * | 2019-11-15 | 2021-05-18 | 上海细胞治疗集团有限公司 | 一种在活化的t细胞中具有高活性的启动子 |
| CN110863008B (zh) * | 2019-11-22 | 2020-10-16 | 昆明学院 | Mar序列调控弱启动子构建高效表达载体的方法 |
| US20230046668A1 (en) | 2019-12-24 | 2023-02-16 | Selexis S.A. | Targeted integration in mammalian sequences enhancing gene expression |
| KR102937659B1 (ko) * | 2020-02-19 | 2026-03-10 | 우시 바이올로직스 아일랜드 리미티드 | 강화된 발현 시스템 및 그 사용방법 |
| CN111500629B (zh) * | 2020-04-17 | 2021-11-19 | 安徽中盛溯源生物科技有限公司 | 一种高表达层粘连蛋白-511变体的方法及其应用 |
| US20230242936A1 (en) * | 2020-05-14 | 2023-08-03 | Seattle Children's Hospital (dba Seattle Children's Research Institute) | Dual promoter systems |
| US12173304B2 (en) * | 2020-07-08 | 2024-12-24 | Neuracle Genetics Inc. | Intron fragments |
| CN113881703B (zh) * | 2021-10-11 | 2022-06-21 | 中国人民解放军军事科学院军事医学研究院 | 一种提高cho细胞同源重组效率的方法及其相关产品和应用 |
| CN116440269B (zh) * | 2022-01-10 | 2024-02-09 | 华中农业大学 | Slc35a1基因作为靶点在防治猪流行性腹泻病中的应用 |
| CN114958914B (zh) * | 2022-06-06 | 2023-09-26 | 新乡医学院 | 一种人类及哺乳动物细胞附着体表达载体、构建方法和应用 |
| CN115896227A (zh) * | 2022-12-20 | 2023-04-04 | 西北农林科技大学 | 一种鉴定外源基因整合位点以增强转基因表达的方法 |
| GB202305637D0 (en) | 2023-04-17 | 2023-05-31 | Macomics Ltd | Transposons, vectors and genetically engineered cells |
| GB202415693D0 (en) | 2024-10-24 | 2024-12-11 | Macomics Ltd | Genetically-engineered cells |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5731178A (en) | 1990-03-21 | 1998-03-24 | Behringwerke Aktiengesellschaft | Attachment-elements for stimulation of eukaryotic expression systems |
| JP3656277B2 (ja) * | 1995-05-17 | 2005-06-08 | 味の素株式会社 | 組換えｄｎａ法によるトランスグルタミナーゼの効率的製造法 |
| DK1395669T3 (da) | 2001-01-26 | 2009-11-16 | Selexis Sa | Matriks bindingsregioner og fremgangsmåder til anvendelse af disse |
| US20040235011A1 (en) * | 2002-06-26 | 2004-11-25 | Cooper Richard K. | Production of multimeric proteins |
| WO2004065581A2 (en) * | 2003-01-15 | 2004-08-05 | Discovery Genomics, Inc. | Transposon-insulator element delivery systems |
| US7244616B2 (en) * | 2003-06-27 | 2007-07-17 | Bayer Pharmaceuticals Corporation | Use of molecular chaperones for the enhanced production of secreted, recombinant proteins in mammalian cells |
| KR101269656B1 (ko) | 2003-10-24 | 2013-05-30 | 셀렉시스 에스. 에이. | Ｍａｒ 서열의 복합 트랜스펙션 방법에 의한 포유동물 세포에서의 고효율 유전자 전달 및 발현 |
| ES2292271B1 (es) * | 2004-05-20 | 2009-02-16 | Proyecto De Biomedicina Cima, S.L. | Un vector hibrido adenovirus-alfavirus para la administracion eficaz y expresion de genes terapeuticos en celulas tumorales. |
| WO2007021353A2 (en) * | 2005-06-10 | 2007-02-22 | Genentech, Inc. | Improving antibody expression using vectors containing insulator elements |
| DK2205618T3 (en) | 2007-09-26 | 2017-02-20 | Intrexon Corp | SYNTHETIC 5 'NON-TRANSLATED REGIONS, EXPRESSION VECTORS AND PROCEDURES FOR INCREASING TRANSGENIC EXPRESSION |
| US20090247609A1 (en) | 2007-12-20 | 2009-10-01 | Hitto Kaufmann | Sm-protein based secretion engineering |
| WO2010008564A2 (en) * | 2008-07-16 | 2010-01-21 | Recombinetics | Plaice dna transposon system |
| EP2417263B1 (en) * | 2009-04-09 | 2015-09-23 | ProteoVec Holding L.L.C. | Production of proteins using transposon-based vectors |
| CN102575264A (zh) * | 2009-09-18 | 2012-07-11 | 瑟莱克斯公司 | 增加的转基因表达和加工的产品和方法 |
-
2014
- 2014-02-01 DK DK14708935.3T patent/DK2951309T3/en active
- 2014-02-01 US US14/761,460 patent/US11898154B2/en active Active
- 2014-02-01 KR KR1020157022393A patent/KR102235603B1/ko active Active
- 2014-02-01 SI SI201431115T patent/SI2951309T1/sl unknown
- 2014-02-01 CN CN202510831703.7A patent/CN120718957A/zh active Pending
- 2014-02-01 WO PCT/IB2014/000100 patent/WO2014118619A2/en not_active Ceased
- 2014-02-01 PL PL19153376.9T patent/PL3536797T3/pl unknown
- 2014-02-01 RU RU2015127667A patent/RU2711505C9/ru active
- 2014-02-01 DK DK19153376.9T patent/DK3536797T5/da active
- 2014-02-01 PT PT14708935T patent/PT2951309T/pt unknown
- 2014-02-01 HU HUE19153376A patent/HUE061494T2/hu unknown
- 2014-02-01 PL PL14708935T patent/PL2951309T3/pl unknown
- 2014-02-01 ES ES14708935T patent/ES2717175T3/es active Active
- 2014-02-01 CA CA2898878A patent/CA2898878C/en active Active
- 2014-02-01 CN CN201480010305.9A patent/CN105073995A/zh active Pending
- 2014-02-01 JP JP2015555816A patent/JP6563816B2/ja active Active
- 2014-02-01 LT LTEP14708935.3T patent/LT2951309T/lt unknown
- 2014-02-01 PT PT191533769T patent/PT3536797T/pt unknown
- 2014-02-01 SG SG10201803758XA patent/SG10201803758XA/en unknown
- 2014-02-01 EP EP14708935.3A patent/EP2951309B1/en active Active
- 2014-02-01 ES ES19153376T patent/ES2940433T3/es active Active
- 2014-02-01 HU HUE14708935A patent/HUE043103T2/hu unknown
- 2014-02-01 FI FIEP19153376.9T patent/FI3536797T3/fi active
- 2014-02-01 SG SG11201505487UA patent/SG11201505487UA/en unknown
- 2014-02-01 AU AU2014210856A patent/AU2014210856B2/en active Active
- 2014-02-01 EP EP19153376.9A patent/EP3536797B1/en active Active
-
2015
- 2015-07-28 IL IL24019815A patent/IL240198B/en active IP Right Grant
- 2015-09-01 ZA ZA2015/06419A patent/ZA201506419B/en unknown
-
2019
- 2019-10-18 AU AU2019250224A patent/AU2019250224B2/en active Active
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