PL2970948T3 - Sposoby oczyszczania rna - Google Patents
Sposoby oczyszczania rnaInfo
- Publication number
- PL2970948T3 PL2970948T3 PL14709704T PL14709704T PL2970948T3 PL 2970948 T3 PL2970948 T3 PL 2970948T3 PL 14709704 T PL14709704 T PL 14709704T PL 14709704 T PL14709704 T PL 14709704T PL 2970948 T3 PL2970948 T3 PL 2970948T3
- Authority
- PL
- Poland
- Prior art keywords
- purification methods
- rna purification
- rna
- methods
- purification
- Prior art date
Links
- 238000000034 method Methods 0.000 title 1
- 238000000746 purification Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P37/00—Drugs for immunological or allergic disorders
- A61P37/02—Immunomodulators
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3847—Multimodal interactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D61/00—Processes of separation using semi-permeable membranes, e.g. dialysis, osmosis or ultrafiltration; Apparatus, accessories or auxiliary operations specially adapted therefor
- B01D61/14—Ultrafiltration; Microfiltration
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H1/00—Processes for the preparation of sugar derivatives
- C07H1/06—Separation; Purification
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
- C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
- C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
- B01D15/3804—Affinity chromatography
- B01D15/3828—Ligand exchange chromatography, e.g. complexation, chelation or metal interaction chromatography
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Crystallography & Structural Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Water Supply & Treatment (AREA)
- Immunology (AREA)
- Medicinal Chemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Saccharide Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Treatment Of Liquids With Adsorbents In General (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201361799705P | 2013-03-15 | 2013-03-15 | |
| EP14709704.2A EP2970948B1 (en) | 2013-03-15 | 2014-03-13 | Rna purification methods |
| PCT/EP2014/055014 WO2014140211A1 (en) | 2013-03-15 | 2014-03-13 | Rna purification methods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| PL2970948T3 true PL2970948T3 (pl) | 2019-06-28 |
Family
ID=50272650
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| PL14709704T PL2970948T3 (pl) | 2013-03-15 | 2014-03-13 | Sposoby oczyszczania rna |
Country Status (17)
| Country | Link |
|---|---|
| US (3) | US11155572B2 (pl) |
| EP (2) | EP2970948B1 (pl) |
| JP (1) | JP6461830B2 (pl) |
| CN (1) | CN105283548A (pl) |
| BR (1) | BR112015023513A2 (pl) |
| CA (1) | CA2906281A1 (pl) |
| CY (1) | CY1121478T1 (pl) |
| DK (1) | DK2970948T3 (pl) |
| ES (2) | ES2923018T3 (pl) |
| HR (1) | HRP20190197T1 (pl) |
| HU (1) | HUE042072T2 (pl) |
| LT (1) | LT2970948T (pl) |
| PL (1) | PL2970948T3 (pl) |
| PT (1) | PT2970948T (pl) |
| SI (1) | SI2970948T1 (pl) |
| TR (1) | TR201903248T4 (pl) |
| WO (1) | WO2014140211A1 (pl) |
Families Citing this family (53)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9464124B2 (en) | 2011-09-12 | 2016-10-11 | Moderna Therapeutics, Inc. | Engineered nucleic acids and methods of use thereof |
| KR20150128687A (ko) | 2013-03-14 | 2015-11-18 | 샤이어 휴먼 지네틱 테라피즈 인크. | 메신저 rna의 정제 방법 |
| US11377470B2 (en) | 2013-03-15 | 2022-07-05 | Modernatx, Inc. | Ribonucleic acid purification |
| US11155572B2 (en) * | 2013-03-15 | 2021-10-26 | Glaxosmithkline Biologicals Sa | RNA purification methods |
| US10023626B2 (en) | 2013-09-30 | 2018-07-17 | Modernatx, Inc. | Polynucleotides encoding immune modulating polypeptides |
| EP3052521A1 (en) | 2013-10-03 | 2016-08-10 | Moderna Therapeutics, Inc. | Polynucleotides encoding low density lipoprotein receptor |
| RU2021109685A (ru) | 2014-04-23 | 2021-04-13 | МОДЕРНАТиЭкс, ИНК. | Вакцины на основе нуклеиновых кислот |
| KR102739894B1 (ko) | 2014-04-25 | 2024-12-05 | 샤이어 휴먼 지네틱 테라피즈 인크. | 메신저 rna 의 정제 방법 |
| EP3294885B1 (en) * | 2015-05-08 | 2020-07-01 | CureVac Real Estate GmbH | Method for producing rna |
| WO2016193206A1 (en) | 2015-05-29 | 2016-12-08 | Curevac Ag | A method for producing and purifying rna, comprising at least one step of tangential flow filtration |
| WO2017025120A1 (en) | 2015-08-07 | 2017-02-16 | Curevac Ag | Process for the in vivo production of rna in a host cell |
| US11162880B2 (en) | 2015-11-09 | 2021-11-02 | University Of Notre Dame Du Lac | Particle size purification method and devices |
| SG11201805217XA (en) | 2015-12-28 | 2018-07-30 | Novartis Ag | Compositions and methods for the treatment of hemoglobinopathies |
| US12385034B2 (en) | 2016-06-24 | 2025-08-12 | Modernatx, Inc. | Methods and apparatus for filtration |
| RU2768045C2 (ru) * | 2016-07-21 | 2022-03-23 | Спарк Терапьютикс, Инк. | СПОСОБ ПОЛУЧЕНИЯ ЧАСТИЦ РЕКОМБИНАНТНОГО АДЕНОАССОЦИИРОВАННОГО ВИРУСНОГО ВЕКТОРА (rAAV) |
| WO2018041921A1 (en) | 2016-08-31 | 2018-03-08 | Curevac Ag | Mixing device for the production of a liquid nucleic acid composition |
| CA3036831A1 (en) | 2016-09-14 | 2018-03-22 | Modernatx, Inc. | High purity rna compositions and methods for preparation thereof |
| WO2018096179A1 (en) | 2016-11-28 | 2018-05-31 | Curevac Ag | Method for purifying rna |
| EP3555289A1 (en) | 2016-12-13 | 2019-10-23 | ModernaTX, Inc. | Rna affinity purification |
| TW201839136A (zh) | 2017-02-06 | 2018-11-01 | 瑞士商諾華公司 | 治療血色素異常症之組合物及方法 |
| WO2018157133A1 (en) * | 2017-02-27 | 2018-08-30 | Translate Bio, Inc. | Methods for purification of messenger rna |
| EP3625363A1 (en) | 2017-05-17 | 2020-03-25 | CureVac Real Estate GmbH | Method for determining at least one quality parameter of an rna sample |
| US11786607B2 (en) | 2017-06-15 | 2023-10-17 | Modernatx, Inc. | RNA formulations |
| EP3665306B1 (en) | 2017-08-11 | 2022-10-19 | GlaxoSmithKline Biologicals SA | Rna identity method using rnase h digestion and size fractionating |
| MA49914A (fr) | 2017-08-18 | 2021-04-21 | Modernatx Inc | Procédés analytiques par hplc |
| EP3668979A4 (en) | 2017-08-18 | 2021-06-02 | Modernatx, Inc. | METHOD OF HPLC ANALYSIS |
| AU2018326799A1 (en) | 2017-08-31 | 2020-02-27 | Modernatx, Inc. | Methods of making lipid nanoparticles |
| IL316292A (en) * | 2017-12-07 | 2024-12-01 | Emp Biotech Gmbh | System and methods for applied radial chromatography technology |
| US12371646B2 (en) * | 2018-06-28 | 2025-07-29 | CureVac Manufacturing GmbH | Bioreactor for RNA in vitro transcription |
| WO2020041793A1 (en) | 2018-08-24 | 2020-02-27 | Translate Bio, Inc. | Methods for purification of messenger rna |
| US12090235B2 (en) | 2018-09-20 | 2024-09-17 | Modernatx, Inc. | Preparation of lipid nanoparticles and methods of administration thereof |
| CN112771062B (zh) * | 2018-10-24 | 2024-01-16 | 豪夫迈·罗氏有限公司 | 用于纯化寡核苷酸的方法 |
| EP3918065A4 (en) * | 2019-01-29 | 2022-10-26 | Flagship Pioneering Innovations V, Inc. | PROCEDURE FOR SEPARATION OF LONG POLYNUCLEOTIDS FROM A COMPOSITION |
| CN113396219A (zh) | 2019-02-11 | 2021-09-14 | 埃泽瑞斯公司 | 通过切向流过滤纯化mRNA |
| CN113874502A (zh) | 2019-03-11 | 2021-12-31 | 摩登纳特斯有限公司 | 补料分批体外转录方法 |
| US20220306678A1 (en) * | 2019-06-20 | 2022-09-29 | Arcturus Therapeutics, Inc. | Improved in vitro transcription purification platform |
| WO2021123920A1 (en) | 2019-12-18 | 2021-06-24 | Novartis Ag | Compositions and methods for the treatment of hemoglobinopathies |
| US12194089B2 (en) | 2020-02-04 | 2025-01-14 | CureVac SE | Coronavirus vaccine |
| IL295757A (en) * | 2020-02-21 | 2022-10-01 | Biogen Ma Inc | Methods for preparing oligonucleotide preparations using ultrafiltration/difiltration |
| JP7789698B2 (ja) * | 2020-05-12 | 2025-12-22 | ハラランボス・マカソリス | Ｒｎａを用いた治療薬の合成方法及び合成モジュール式装置 |
| CA3178965A1 (en) | 2020-05-15 | 2021-11-18 | Christian COBAUGH | Messenger rna encoding cas9 for use in genome-editing systems |
| CN115715324A (zh) * | 2020-06-19 | 2023-02-24 | 伊泽阿恩埃免疫疗法股份有限公司 | Rna纯化方法 |
| CN111812230A (zh) * | 2020-06-24 | 2020-10-23 | 南京济群生物科技有限公司 | 一种半定量测定dna中rna含量的sec-hplc检测方法 |
| IL301301A (en) * | 2020-09-14 | 2023-05-01 | Enquyst Tech Inc | Process technology for the production of biological products and downstream purification |
| US12329811B2 (en) | 2021-01-11 | 2025-06-17 | Modernatx, Inc. | Seasonal RNA influenza virus vaccines |
| US20220364078A1 (en) * | 2021-05-14 | 2022-11-17 | Crispr Therapeutics Ag | Mrna large scale synthesis and purification |
| US20240218353A1 (en) * | 2021-06-17 | 2024-07-04 | Modernatx, Inc. | Alternative rna purification strategies |
| EP4359527A2 (en) | 2021-06-23 | 2024-05-01 | Novartis AG | Compositions and methods for the treatment of hemoglobinopathies |
| EP4396342A1 (en) | 2021-09-01 | 2024-07-10 | GlaxoSmithKline Biologicals S.A. | Plasmid dna purification methods |
| US20250027108A1 (en) | 2021-10-29 | 2025-01-23 | CureVac SE | Improved circular rna for expressing therapeutic proteins |
| IL318619A (en) | 2022-07-29 | 2025-03-01 | Sanofi Pasteur Inc | Methods for ethanol-free purification of mRNA |
| WO2024072904A2 (en) * | 2022-09-28 | 2024-04-04 | Modernatx, Inc. | Purification of nucleotides |
| WO2025186218A1 (en) | 2024-03-04 | 2025-09-12 | Cytiva Bioprocess R&D Ab | Purification and analysis of rna |
Family Cites Families (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5256294A (en) | 1990-09-17 | 1993-10-26 | Genentech, Inc. | Tangential flow filtration process and apparatus |
| US6011148A (en) | 1996-08-01 | 2000-01-04 | Megabios Corporation | Methods for purifying nucleic acids |
| EP0853123A1 (de) | 1997-01-10 | 1998-07-15 | Roche Diagnostics GmbH | Reinigung von DNA durch Cross-Flow-Filtration |
| US6355792B1 (en) | 1998-02-04 | 2002-03-12 | Merck Patent Gesellschaft | Method for isolating and purifying nucleic acids |
| CA2457565A1 (en) * | 2001-10-09 | 2003-04-17 | Genentech, Inc. | Novel acidic mammalian proteins and polynucleotides encoding the same |
| US8075780B2 (en) | 2003-11-24 | 2011-12-13 | Millipore Corporation | Purification and concentration of synthetic biological molecules |
| AU2005245956B2 (en) | 2004-05-18 | 2011-05-19 | Alphavax, Inc. | TC-83-derived alphavirus vectors, particles and methods |
| WO2008073620A2 (en) * | 2006-11-02 | 2008-06-19 | Neose Technologies, Inc. | Manufacturing process for the production of polypeptides expressed in insect cell-lines |
| DE102006061015A1 (de) | 2006-12-22 | 2008-06-26 | Curevac Gmbh | Verfahren zur Reinigung von RNA im präparativen Maßstab mittels HPLC |
| WO2011005799A2 (en) | 2009-07-06 | 2011-01-13 | Novartis Ag | Self replicating rna molecules and uses thereof |
| US9192661B2 (en) | 2010-07-06 | 2015-11-24 | Novartis Ag | Delivery of self-replicating RNA using biodegradable polymer particles |
| JP5940064B2 (ja) | 2010-07-06 | 2016-06-29 | ノバルティス アーゲー | 低用量のｒｎａを用いた大型哺乳動物の免疫化 |
| DK2590676T3 (en) | 2010-07-06 | 2016-10-24 | Glaxosmithkline Biologicals Sa | Virionlignende feed particles to self-replicating RNA molecules |
| NZ606591A (en) | 2010-07-06 | 2015-02-27 | Novartis Ag | Cationic oil-in-water emulsions |
| EP2593546A4 (en) * | 2010-07-16 | 2014-01-01 | Valorisation Recherche Ltd Partnership | AFFINITY CLEANING OF RNA UNDER NATIVE CONDITIONS BASED ON LAMBDA BOX B / N PEPTIDE INTERACTION |
| BR112013004865A2 (pt) | 2010-08-31 | 2016-06-07 | Novartis Ag | lípidos adequados para entrega lipossomal de codificadores de proteínas rna |
| SMT202200311T1 (it) | 2010-08-31 | 2022-09-14 | Glaxosmithkline Biologicals Sa | Liposomi pegilati per somministrare rna codificante un immunogene |
| HRP20221048T1 (hr) | 2010-08-31 | 2022-11-11 | Glaxosmithkline Biologicals Sa | Mali liposomi za isporuku rna koja kodira imunogen |
| US20130337528A1 (en) | 2010-12-10 | 2013-12-19 | Tracy Thompson | Compositions for separation methods |
| EP3854413A1 (en) | 2011-07-06 | 2021-07-28 | GlaxoSmithKline Biologicals SA | Immunogenic combination compositions and uses thereof |
| US20140141070A1 (en) | 2011-07-06 | 2014-05-22 | Andrew Geall | Liposomes having useful n:p ratio for delivery of rna molecules |
| CN103781470A (zh) | 2011-07-06 | 2014-05-07 | 诺华股份有限公司 | 包含核酸的水包油乳液 |
| EP3456316A1 (en) | 2011-07-06 | 2019-03-20 | GlaxoSmithKline Biologicals S.A. | Cationic oil-in-water emulsions |
| WO2013103439A1 (en) * | 2012-01-06 | 2013-07-11 | Infinity Fuel Cell And Hydrogen, Inc. | Electrochemical cell with improved water or gas management |
| KR20150128687A (ko) | 2013-03-14 | 2015-11-18 | 샤이어 휴먼 지네틱 테라피즈 인크. | 메신저 rna의 정제 방법 |
| US11155572B2 (en) * | 2013-03-15 | 2021-10-26 | Glaxosmithkline Biologicals Sa | RNA purification methods |
-
2014
- 2014-03-13 US US14/775,760 patent/US11155572B2/en active Active
- 2014-03-13 PT PT14709704T patent/PT2970948T/pt unknown
- 2014-03-13 SI SI201431108T patent/SI2970948T1/sl unknown
- 2014-03-13 WO PCT/EP2014/055014 patent/WO2014140211A1/en not_active Ceased
- 2014-03-13 CN CN201480015831.4A patent/CN105283548A/zh active Pending
- 2014-03-13 BR BR112015023513A patent/BR112015023513A2/pt not_active Application Discontinuation
- 2014-03-13 HR HRP20190197TT patent/HRP20190197T1/hr unknown
- 2014-03-13 LT LTEP14709704.2T patent/LT2970948T/lt unknown
- 2014-03-13 EP EP14709704.2A patent/EP2970948B1/en active Active
- 2014-03-13 CA CA2906281A patent/CA2906281A1/en active Pending
- 2014-03-13 TR TR2019/03248T patent/TR201903248T4/tr unknown
- 2014-03-13 DK DK14709704.2T patent/DK2970948T3/en active
- 2014-03-13 ES ES18212923T patent/ES2923018T3/es active Active
- 2014-03-13 ES ES14709704T patent/ES2720200T3/es active Active
- 2014-03-13 PL PL14709704T patent/PL2970948T3/pl unknown
- 2014-03-13 HU HUE14709704A patent/HUE042072T2/hu unknown
- 2014-03-13 EP EP18212923.9A patent/EP3521429B1/en active Active
- 2014-03-13 JP JP2015562160A patent/JP6461830B2/ja active Active
-
2019
- 2019-03-12 CY CY20191100284T patent/CY1121478T1/el unknown
-
2021
- 2021-01-25 US US17/157,150 patent/US12018044B2/en active Active
-
2024
- 2024-05-15 US US18/664,453 patent/US20240300994A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP2970948B1 (en) | 2018-12-26 |
| CN105283548A (zh) | 2016-01-27 |
| US20160024139A1 (en) | 2016-01-28 |
| EP2970948A1 (en) | 2016-01-20 |
| DK2970948T3 (en) | 2019-04-08 |
| HRP20190197T1 (hr) | 2019-04-05 |
| EP3521429A1 (en) | 2019-08-07 |
| PT2970948T (pt) | 2019-03-20 |
| JP2016512212A (ja) | 2016-04-25 |
| ES2720200T3 (es) | 2019-07-18 |
| SI2970948T1 (sl) | 2019-04-30 |
| CA2906281A1 (en) | 2014-09-18 |
| TR201903248T4 (tr) | 2019-03-21 |
| HUE042072T2 (hu) | 2019-06-28 |
| BR112015023513A2 (pt) | 2017-07-18 |
| CY1121478T1 (el) | 2020-05-29 |
| ES2923018T3 (es) | 2022-09-22 |
| LT2970948T (lt) | 2019-03-25 |
| US20240300994A1 (en) | 2024-09-12 |
| JP6461830B2 (ja) | 2019-01-30 |
| WO2014140211A1 (en) | 2014-09-18 |
| US11155572B2 (en) | 2021-10-26 |
| EP3521429B1 (en) | 2022-05-04 |
| US12018044B2 (en) | 2024-06-25 |
| US20210214388A1 (en) | 2021-07-15 |
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