PL2336172T3 - Usuwanie agregatów o dużej masie cząsteczkowej przy użyciu chromatografii hydroksyapatytowej - Google Patents

Usuwanie agregatów o dużej masie cząsteczkowej przy użyciu chromatografii hydroksyapatytowej

Info

Publication number
PL2336172T3
PL2336172T3 PL10174366T PL10174366T PL2336172T3 PL 2336172 T3 PL2336172 T3 PL 2336172T3 PL 10174366 T PL10174366 T PL 10174366T PL 10174366 T PL10174366 T PL 10174366T PL 2336172 T3 PL2336172 T3 PL 2336172T3
Authority
PL
Poland
Prior art keywords
removal
molecular weight
high molecular
hydroxyapatite chromatography
weight aggregates
Prior art date
Application number
PL10174366T
Other languages
English (en)
Polish (pl)
Inventor
Shujun Sun
Christopher Gallo
Brian Kelley
Original Assignee
Wyeth Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Llc filed Critical Wyeth Llc
Publication of PL2336172T3 publication Critical patent/PL2336172T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3847—Multimodal interactions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
    • B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
    • B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
    • B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
    • B01J20/282—Porous sorbents
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2851—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction, e.g. ion-exchange, ion-pair, ion-suppression or ion-exclusion
    • B01D15/361—Ion-exchange
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
    • B01D15/08—Selective adsorption, e.g. chromatography
    • B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
    • B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 and B01D15/30 - B01D15/36, e.g. affinity, ligand exchange or chiral chromatography
    • B01D15/3804—Affinity chromatography
    • B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G or L chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
PL10174366T 2003-10-27 2004-10-06 Usuwanie agregatów o dużej masie cząsteczkowej przy użyciu chromatografii hydroksyapatytowej PL2336172T3 (pl)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US51401803P 2003-10-27 2003-10-27
US52333503P 2003-11-20 2003-11-20
EP04794288.3A EP1678208B1 (en) 2003-10-27 2004-10-06 Removal of high molecular weight aggregates using hydroxyapatite chromatography
EP10174366.4A EP2336172B1 (en) 2003-10-27 2004-10-06 Removal of high molecular weight aggregates using hydroxyapatite chromatography

Publications (1)

Publication Number Publication Date
PL2336172T3 true PL2336172T3 (pl) 2015-04-30

Family

ID=34576743

Family Applications (2)

Application Number Title Priority Date Filing Date
PL10174366T PL2336172T3 (pl) 2003-10-27 2004-10-06 Usuwanie agregatów o dużej masie cząsteczkowej przy użyciu chromatografii hydroksyapatytowej
PL04794288T PL1678208T3 (pl) 2003-10-27 2004-10-06 Usuwanie agregatów o wysokiej masie cząsteczkowej z użyciem chromatografii na hydroksyapatycie

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL04794288T PL1678208T3 (pl) 2003-10-27 2004-10-06 Usuwanie agregatów o wysokiej masie cząsteczkowej z użyciem chromatografii na hydroksyapatycie

Country Status (19)

Country Link
US (2) US9469672B2 (enExample)
EP (2) EP2336172B1 (enExample)
JP (2) JP5004586B2 (enExample)
KR (2) KR101221862B1 (enExample)
CN (2) CN1898266B (enExample)
AU (1) AU2004286938B2 (enExample)
BR (2) BRPI0415887B1 (enExample)
CA (1) CA2543193C (enExample)
CO (1) CO5690649A2 (enExample)
DK (2) DK2336172T3 (enExample)
EC (1) ECSP066583A (enExample)
ES (2) ES2418830T3 (enExample)
IL (1) IL175229A (enExample)
MX (1) MXPA06004472A (enExample)
NZ (1) NZ547315A (enExample)
PL (2) PL2336172T3 (enExample)
PT (2) PT2336172E (enExample)
RU (2) RU2409591C2 (enExample)
WO (1) WO2005044856A2 (enExample)

Families Citing this family (71)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0304576D0 (en) * 2003-02-28 2003-04-02 Lonza Biologics Plc Protein a chromatography
DK2336172T3 (en) * 2003-10-27 2015-01-26 Wyeth Llc The removal of aggregates of high molecular weight by hydroxylapatite chromatography
CN104610422A (zh) * 2005-03-11 2015-05-13 惠氏公司 弱分配层析的方法
CN101213211A (zh) 2005-06-17 2008-07-02 惠氏公司 纯化含Fc区蛋白的方法
EP1893647A2 (en) * 2005-06-23 2008-03-05 MedImmune, Inc. Antibody formulations having optimized aggregation and fragmentation profiles
AR058140A1 (es) * 2005-10-24 2008-01-23 Wyeth Corp Metodo de produccion proteica utilizando compuestos anti-senescencia
JP5405122B2 (ja) * 2005-12-21 2014-02-05 ワイス・エルエルシー 低粘度のタンパク質製剤およびその用途
US8088833B2 (en) 2006-04-25 2012-01-03 Tosoh Corporation Method for purifying an IgG monomer
EP2069387A4 (en) * 2006-06-14 2011-02-02 Glaxosmithkline Llc METHODS OF PURIFYING ANTIBODIES USING HYDROXYAPATITE CERAMIC
LT2061803T (lt) * 2006-08-28 2019-11-25 Ares Trading Sa Fc turinčių baltymų gryninimo būdas
ZA200900836B (en) * 2006-08-28 2010-05-26 Ares Trading Sa Process for the purification of FC-fusion proteins
CL2007002615A1 (es) * 2006-09-08 2008-04-18 Wyeth Corp Metodos para aislar o purificar un producto que comprende poner el producto enlazado en contacto con al menos una solucion de lavado que comprende arginina y luego eluir el producto; y dicho producto.
MX345141B (es) 2006-09-13 2017-01-18 Abbvie Inc * Mejoras de cultivos celulares.
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
US7999085B2 (en) * 2007-01-09 2011-08-16 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US7691980B2 (en) * 2007-01-09 2010-04-06 Bio-Rad Laboratories, Inc. Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers
US20100063257A1 (en) * 2007-01-26 2010-03-11 Merck Serono Sa Purification of FC-TACI Fusion Proteins Using the Oilbody Technology
TW200902708A (en) 2007-04-23 2009-01-16 Wyeth Corp Methods of protein production using anti-senescence compounds
KR101163307B1 (ko) * 2007-10-26 2012-07-05 아사히 가세이 케미칼즈 가부시키가이샤 단백질의 정제 방법
US8188242B2 (en) * 2008-04-08 2012-05-29 Bio-Rad Laboratories, Inc. Chromatography purification of antibodies
HUE047316T2 (hu) † 2008-08-14 2020-04-28 Genentech Inc Eljárások szennyezés eltávolítására indigén fehérjekiszorításos ioncserés membrán kromatográfia alkalmazásával
TWI610936B (zh) * 2008-10-20 2018-01-11 艾伯維有限公司 使用蛋白質ａ親和性層析進行抗體之分離及純化
CN104974251A (zh) 2008-10-20 2015-10-14 Abbvie公司 在抗体纯化过程中的病毒灭活
US9393304B2 (en) 2008-10-29 2016-07-19 Ablynx N.V. Formulations of single domain antigen binding molecules
CN118324847A (zh) 2008-10-29 2024-07-12 阿布林克斯公司 单域抗原结合性分子的纯化方法
WO2010051360A1 (en) * 2008-10-31 2010-05-06 Wyeth Llc Purification of acidic proteins using ceramic hydroxyapatite chromatography
BRPI1009447A2 (pt) * 2009-03-11 2016-03-01 Wyeth Llc métodos de purificação de proteínas imunofarmacêuticas modulares pequenas
CA2759502C (en) 2009-04-29 2021-02-09 Bio-Rad Laboratories, Inc. Purification of immunoconjugates
JPWO2011001963A1 (ja) 2009-07-03 2012-12-13 旭化成ケミカルズ株式会社 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法
BR112012009289B8 (pt) 2009-10-20 2021-05-25 Abbott Laboratoires método para purificar um anticorpo anti-il-13 a partir de uma mistura de amostra que compreende um anticorpo anti-il-13 e pelo menos uma proteína de célula hospedeira (hcp)
BR112012014937B1 (pt) 2009-12-18 2021-01-19 Novartis Ag método de produção de um anticorpo, fragmento de anticorpo ou proteína de fusão fc purificada usando uma matriz de cromatografia de afinidade
WO2011090720A2 (en) * 2009-12-29 2011-07-28 Dr. Reddy's Laboratories Ltd Purification of proteins
US8951807B2 (en) * 2010-01-15 2015-02-10 Bio-Rad Laboratories, Inc. Surface neutralization of apatite
EP2558492B1 (en) * 2010-04-14 2016-11-16 F.Hoffmann-La Roche Ag Immunoglobulin aggregate removal
GB201006753D0 (en) 2010-04-22 2010-06-09 Biotest Ag Process for preparing an immunolobulin composition
US20110301333A1 (en) * 2010-06-08 2011-12-08 Millipore Corporation Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts
US8895707B2 (en) * 2010-08-18 2014-11-25 Bio-Rad Laboratories, Inc. Elution of proteins from hydroxyapatite resins without resin deterioration
EP2635599A1 (en) * 2010-11-01 2013-09-11 DSM IP Assets B.V. Single unit ion exchange chromatography antibody purification
WO2012106449A1 (en) 2011-02-02 2012-08-09 Bio-Rad Laboratories, Inc. Apatite surface neutralization with alkali solutions
SG10201603931TA (en) * 2011-06-08 2016-07-28 Agency Science Tech & Res Purification Of Biological Products By Constrained Cohydration Chromatography
AU2012269240B2 (en) * 2011-06-16 2016-01-21 Dpx Holdings B.V. Single unit chromatography antibody purification
SG10201701224UA (en) * 2012-03-12 2017-04-27 Merck Patent Gmbh Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode
WO2013180933A1 (en) 2012-05-30 2013-12-05 Bio-Rad Laboratories, Inc. In situ restoration of apatite-based chromatography resins
WO2014003142A1 (ja) * 2012-06-27 2014-01-03 旭化成メディカル株式会社 抗体
JP2014105180A (ja) * 2012-11-27 2014-06-09 Hoya Corp モノマー化方法
KR102236926B1 (ko) 2013-03-13 2021-04-06 세센 바이오, 아이엔씨. 안구 운반을 위한 키메릭 사이토카인 제제
WO2014209508A1 (en) * 2013-05-13 2014-12-31 Medimmune, Llc Separation of recombinant polyclonal antibody multimers with minimal separation of monomers
MX384222B (es) 2013-07-05 2025-03-14 Lab Francais Du Fractionnement Matriz de cromatografia de afinidad.
CA2910770C (en) 2013-07-12 2018-02-27 Merck Patent Gmbh Removal of fragments from a sample containing a target protein using activated carbon
JP6479305B2 (ja) * 2013-07-12 2019-03-06 株式会社Ｕｍｎファーマ ウイルス様粒子の精製方法
WO2015088677A1 (en) 2013-12-12 2015-06-18 Emd Millipore Corporation Protein separations using an acrylamide containing filter
CN106029682B (zh) 2013-12-27 2021-04-13 中外制药株式会社 等电点低的抗体的纯化方法
EP3157689B1 (en) * 2014-06-23 2021-04-28 Bio-Rad Laboratories, Inc. Apatite pretreatment
WO2015200299A2 (en) 2014-06-23 2015-12-30 Bio-Rad Laboratories, Inc. Apatite in-situ restoration
US10617975B2 (en) 2014-12-15 2020-04-14 Merck Patent Gmbh Target molecule capture from crude solutions
US10695693B2 (en) 2015-03-13 2020-06-30 Bristol-Myers Squibb Company Use of alkaline washes during chromatography to remove impurities
ES2817751T3 (es) 2015-12-21 2021-04-08 Pfizer Purificación de conjugados de anticuerpo-fármaco mediante el uso de un gradiente de fosfato de sodio
US11186858B1 (en) 2016-03-15 2021-11-30 Fresenius Kabi Deutschland Gmbh Methods for increasing biosimilarity
US11919924B1 (en) * 2016-03-15 2024-03-05 Fresenius Kabi Deutschland Gmbh Methods of purifying and producing an adalimumab antibody
CN106977591B (zh) * 2017-05-04 2020-05-05 广州康盛生物科技股份有限公司 一种分离纯化重组葡萄球菌蛋白a的方法
EP3672981A4 (en) 2017-08-22 2021-09-15 Biogen MA Inc. PROCESSES FOR PURIFYING ANTIBODIES PRESENTING REDUCED AGGREGATES WITH HIGH MOLECULAR WEIGHT
WO2019116096A1 (en) * 2017-12-15 2019-06-20 Laboratoire Francais Du Fractionnement Et Des Biotechnologies Production of fc fragments
JP7449041B2 (ja) * 2018-02-27 2024-03-13 ファイザー・インク 抗体精製
WO2019183334A1 (en) 2018-03-21 2019-09-26 Waters Technologies Corporation Non-antibody high-affinity-based sample preparation, sorbents, devices and methods
WO2019192877A1 (en) 2018-04-03 2019-10-10 Merck Patent Gmbh Cex chromatography media and low salt elution of target proteins from biopharmaceutical feeds
WO2019224660A1 (ja) * 2018-05-24 2019-11-28 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 吸着剤の生産方法
JP6704074B2 (ja) * 2019-02-04 2020-06-03 株式会社Ｕｍｎファーマ ウイルス様粒子の精製方法
KR20220101168A (ko) 2019-11-22 2022-07-19 모르포시스 아게 이온 교환 크로마토그래피 동안 항체의 수율을 증가시키는 방법
JP2020099909A (ja) * 2020-03-17 2020-07-02 ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 処理方法、生産方法およびハイドロキシアパタイト充填剤
MX2022012278A (es) 2020-03-30 2022-10-27 Ablynx Nv Metodo para la produccion y purificacion de dominios variables unicos de inmunoglobulina multivalentes.
EP4142792A4 (en) * 2020-05-01 2024-11-27 Kashiv Biosciences, LLC An improved process of purification of protein

Family Cites Families (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US523335A (en) 1894-07-24 Air-bolt for flour-mills
US428894A (en) 1890-05-27 Process of making tool-handles
US514018A (en) 1894-02-06 Edward j
US419964A (en) 1890-01-21 Transom-lifter
US4745183A (en) * 1984-02-21 1988-05-17 Bio-Rad Laboratories, Inc. Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies
JPH03291300A (ja) * 1989-12-28 1991-12-20 Mitsui Toatsu Chem Inc ポリクローナル抗体の分離方法
GB9022545D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Culture medium
TWI255272B (en) * 2000-12-06 2006-05-21 Guriq Basi Humanized antibodies that recognize beta amyloid peptide
EP1463942B2 (en) * 2001-12-21 2012-06-20 Immunex Corporation Methods for purifying protein
EP1601697B1 (en) 2003-02-28 2007-05-30 Lonza Biologics plc Antibody purification by Protein A and ion exchange chromatography
DK2336172T3 (en) * 2003-10-27 2015-01-26 Wyeth Llc The removal of aggregates of high molecular weight by hydroxylapatite chromatography

Also Published As

Publication number Publication date
RU2573910C2 (ru) 2016-01-27
BR122020007658B1 (pt) 2021-07-27
CN102276717B (zh) 2015-09-09
EP2336172A1 (en) 2011-06-22
JP5004586B2 (ja) 2012-08-22
DK1678208T3 (da) 2013-07-08
BRPI0415887A (pt) 2007-01-09
MXPA06004472A (es) 2006-06-20
CN1898266B (zh) 2011-09-21
EP1678208A2 (en) 2006-07-12
ECSP066583A (es) 2006-10-17
IL175229A (en) 2013-08-29
US9469672B2 (en) 2016-10-18
WO2005044856A2 (en) 2005-05-19
EP1678208B1 (en) 2013-05-15
CN1898266A (zh) 2007-01-17
US9822143B2 (en) 2017-11-21
US20050107594A1 (en) 2005-05-19
JP2007532477A (ja) 2007-11-15
KR20120003027A (ko) 2012-01-09
CO5690649A2 (es) 2006-10-31
PT1678208E (pt) 2013-07-10
JP2012111769A (ja) 2012-06-14
PT2336172E (pt) 2015-02-20
RU2010139827A (ru) 2012-03-27
CA2543193A1 (en) 2005-05-19
AU2004286938B2 (en) 2011-06-30
KR101321876B1 (ko) 2013-10-28
WO2005044856A3 (en) 2005-06-23
EP2336172B1 (en) 2014-12-10
CA2543193C (en) 2015-08-11
CN102276717A (zh) 2011-12-14
ES2418830T3 (es) 2013-08-16
DK2336172T3 (en) 2015-01-26
KR101221862B1 (ko) 2013-01-14
ES2530446T3 (es) 2015-03-02
JP5490156B2 (ja) 2014-05-14
KR20060120163A (ko) 2006-11-24
RU2006113695A (ru) 2007-12-10
BRPI0415887B1 (pt) 2021-01-26
PL1678208T3 (pl) 2013-09-30
IL175229A0 (en) 2006-09-05
US20130197198A1 (en) 2013-08-01
NZ547315A (enExample) 2008-07-31
RU2409591C2 (ru) 2011-01-20
AU2004286938A1 (en) 2005-05-19

Similar Documents

Publication Publication Date Title
IL175229A0 (en) Removal of high molecular weight aggregates using hydroxyapatite chromatography
AU2003268210A8 (en) Chromatographic methods for adenovirus purification
EP1556428A4 (en) COMPOSITIONS CURING RAPIDLY
IL172030A0 (en) Hydrogel composition demonstrating phase separation
EP1630186A4 (en) HARDENING COMPOSITION
PL378134A1 (pl) Nowe pochodne bicykliczne i kompozycje farmaceutyczne zawierające pochodne bicykliczne
AU2003259290A8 (en) Glass compositions
GB0310767D0 (en) Stick compositions
EP1640391A4 (en) HARDENING COMPOSITION
HRP20030824A2 (en) Phenyl heterocyclyl ether derivatives as serotoninre-uptake inhibitors
PT1842550E (pt) Composição para indução de imunotolerância
GB0310771D0 (en) Stick compositions
AU152108S (en) Set of pavers
SI1678208T1 (sl) Odstranjevanje agregatov z visoko molekulsko maso z uporabo hidroksiapatitne kromatografije
IL175678A0 (en) Novel piperidine-substituted indoles-or heteroderivatives thereof
GB0310769D0 (en) Stick compositions
HK1086845A (en) Removal of high molecular weight aggregates using hydroxyapatite chromatography
GB0324574D0 (en) Novel compositions
GB0328023D0 (en) Novel compositions
LT1641933T (lt) Cefradino gamybos būdas
GB0225021D0 (en) Rubber compositions
GB2397354B (en) Mounting apparatus of two members
ZA200506880B (en) Hydrofluorocarbon compositions
HK1064727B (en) Mounting apparatus of two members
GB0314836D0 (en) Novel compositions