PL1809750T3 - Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach - Google Patents

Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach

Info

Publication number
PL1809750T3
PL1809750T3 PL05810935T PL05810935T PL1809750T3 PL 1809750 T3 PL1809750 T3 PL 1809750T3 PL 05810935 T PL05810935 T PL 05810935T PL 05810935 T PL05810935 T PL 05810935T PL 1809750 T3 PL1809750 T3 PL 1809750T3
Authority
PL
Poland
Prior art keywords
selection
host cells
cells expressing
high levels
expressing protein
Prior art date
Application number
PL05810935T
Other languages
English (en)
Polish (pl)
Inventor
Arie Pieter Otte
Blokland Henricus Johannes Maria Van
Theodorus Hendrikus Jacobus Kwaks
Richard George Antonius Bernardus Sewalt
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Publication of PL1809750T3 publication Critical patent/PL1809750T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C12N5/12—Fused cells, e.g. hybridomas
    • C12N5/16—Animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
    • C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
PL05810935T 2004-11-08 2005-11-07 Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach PL1809750T3 (pl)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US62630104P 2004-11-08 2004-11-08
EP04105593 2004-11-08
US69661005P 2005-07-05 2005-07-05
EP05810935A EP1809750B1 (fr) 2004-11-08 2005-11-07 Selection de cellules hotes exprimant une proteine a des niveaux eleves
PCT/EP2005/055794 WO2006048459A2 (fr) 2004-11-08 2005-11-07 Selection de cellules hotes exprimant une proteine a des niveaux eleves

Publications (1)

Publication Number Publication Date
PL1809750T3 true PL1809750T3 (pl) 2012-08-31

Family

ID=36090897

Family Applications (1)

Application Number Title Priority Date Filing Date
PL05810935T PL1809750T3 (pl) 2004-11-08 2005-11-07 Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach

Country Status (14)

Country Link
US (1) US20060141577A1 (fr)
EP (1) EP1809750B1 (fr)
JP (1) JP5291341B2 (fr)
KR (1) KR101271884B1 (fr)
AU (1) AU2005300503B2 (fr)
BR (1) BRPI0517380A (fr)
CA (1) CA2581422C (fr)
EA (1) EA010863B1 (fr)
HK (1) HK1101264A1 (fr)
IL (1) IL183035A0 (fr)
MX (1) MX2007004595A (fr)
NO (1) NO344469B1 (fr)
PL (1) PL1809750T3 (fr)
WO (1) WO2006048459A2 (fr)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5654442A (en) * 1989-11-14 1997-08-05 The Perkin-Elmer Corporation 4,7-dichlorofluorescein dyes as molecular probes
ATE524559T1 (de) * 2002-12-18 2011-09-15 Chromagenics Bv Methode zur verbesserung der proteinproduktion
DE60312039T2 (de) 2002-12-20 2007-11-08 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
ES2384391T3 (es) 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP2484774A3 (fr) 2005-07-21 2012-11-14 Abbott Laboratories Expression de gènes multiples incluant des constructions molles avec des polyprotéines, des pro-polyprotéines et protéolyse
JP5225107B2 (ja) * 2006-02-21 2013-07-03 クロマジェニックス ベー ヴェー 高レベルでタンパク質を発現する宿主細胞の選択
US7968700B2 (en) 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof
EP2064337B1 (fr) * 2006-09-20 2012-07-18 Genzyme Corporation Système à base de facs et de protéine reporteur pour un développement haut débit de protéines thérapeutiques
US20080274137A1 (en) * 2007-05-02 2008-11-06 Jean Christophe Francis Audonnet DNA plasmids having improved expression and stability
US8653249B2 (en) 2008-02-27 2014-02-18 Fuso Pharmaceutical Industries, Ltd. Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof
WO2010026443A1 (fr) * 2008-09-08 2010-03-11 Cellectis Variants de méganucléase clivant une séquence d'adn cible provenant d'un gène de la glutamine synthétase et leurs utilisations
ES2552325T3 (es) * 2008-12-22 2015-11-27 National University Corporation Hokkaido University Vector de expresión para producción a gran escala de proteína derivada de genes foráneos usando células animales, y uso del mismo
CN102459607B (zh) * 2009-06-15 2014-08-06 萨拉基尼克有限公司 新的严格的可选择的标志物
WO2011113841A1 (fr) * 2010-03-16 2011-09-22 Robert Steinfeld Vecteur eucaryote
EP2582816B1 (fr) 2010-06-15 2017-12-20 Cellagenics B.V. Nouvaux éléments intergéniques pour améliorer l'expression de gènes
CN103180443B (zh) 2010-09-01 2016-01-13 萨拉基尼克有限公司 用于增强基因表达的来自核糖体蛋白启动子的核酸片段
CN113151179A (zh) * 2012-07-31 2021-07-23 阿格克斯治疗有限公司 Hla g修饰的细胞及方法
MX2017005788A (es) 2014-11-04 2017-08-02 Janssen Vaccines & Prevention Bv Vacunas terapeuticas contra el vph16.
CA2995740A1 (fr) 2015-08-20 2017-02-23 Janssen Vaccines & Prevention B.V. Vaccins therapeutiques contre le vph 18
WO2017062722A2 (fr) 2015-10-09 2017-04-13 Genzyme Corporation Technologie flare (flow cytometry attenuated reporter expression, expression de reporter atténuée par cytométrie en flux) pour tri en vrac rapide
US10517944B2 (en) 2016-05-02 2019-12-31 Janssen Vaccines & Prevention B.V. Therapeutic HPV vaccine combinations
TWI795373B (zh) 2016-10-07 2023-03-11 美商健臻公司 用於生物製劑生產之細胞早期轉染後分離（ｅｐｉｃ）
US11781146B2 (en) 2018-05-24 2023-10-10 National University Corporation Hokkaido University Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element
US20210292752A1 (en) * 2018-08-13 2021-09-23 Spiber Inc. Method for Isolating or Identifying Cell, and Cell Mass
US20220033845A1 (en) 2018-09-24 2022-02-03 Merck Sharp & Dohme Corp. Expression vectors for eukaryotic expression systems
EP4247947A1 (fr) 2020-11-19 2023-09-27 Genzyme Corporation Système rapporteur multiple à expression rapporteur atténuée par cytométrie de flux et procédés d'utilisation de celui-ci

Family Cites Families (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3700633A (en) * 1971-05-05 1972-10-24 Shell Oil Co Selectively hydrogenated block copolymers
US3821148A (en) * 1973-09-28 1974-06-28 Exxon Research Engineering Co Plasticized thermoplastic block copolymers
US4122134A (en) * 1974-02-13 1978-10-24 Sumitomo Chemical Company, Limited Method for producing transparent block copolymer resin
US3965830A (en) * 1974-12-05 1976-06-29 Usm Corporation Assembly for automatic bar tacking
US4267284A (en) * 1977-01-07 1981-05-12 Phillips Petroleum Company Tough, transparent articles from styrene polymers blended with certain block-random copolymers
US4965196A (en) 1983-01-19 1990-10-23 Genentech, Inc. Polycistronic expression vector construction
FR2569723B1 (fr) * 1984-08-31 1986-09-19 Centre Nat Rech Scient Application des antibiotiques de la famille des phleomycines a titre d'agent de selection dans le domaine du genie genetique
US4835200A (en) * 1986-12-19 1989-05-30 Shell Oil Company Color stable hot melt adhesive
US4882384A (en) * 1988-02-01 1989-11-21 Shell Oil Company Modified block copolymers
WO1991001374A1 (fr) * 1989-07-24 1991-02-07 Seragen, Inc. Prevention d'un commencement de translation interne
US5256568A (en) * 1990-02-12 1993-10-26 Regeneron Phamaceuticals, Inc. Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers
ES2153358T3 (es) 1991-03-29 2001-03-01 Genentech Inc Procedimientos para la seleccion de celulas huespedes recombinantes que expresan altos niveles de una proteina deseada.
US6319707B1 (en) * 1991-08-12 2001-11-20 Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington Cap-independent multicistronic retroviral vectors
JP2686025B2 (ja) * 1992-08-25 1997-12-08 理化学研究所 ブラストサイジンｓデアミナーゼ遺伝子
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
CA2149326C (fr) 1992-11-13 2007-04-17 Mitchell E. Reff Sequences consensus kozak entierement alterees pour expression par des cellules de mammiferes
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
WO1994023046A1 (fr) 1993-04-07 1994-10-13 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Sequence adn agissant comme un element d'isolement de la chromatine pour proteger des genes exprimes de sequences regulatoires a action cis dans des cellules mammaliennes
US5561053A (en) 1994-08-05 1996-10-01 Genentech, Inc. Method for selecting high-expressing host cells
US5627033A (en) 1995-03-28 1997-05-06 Research & Diagnostics Systems, Inc. Mammalian expression vectors
US5994128A (en) 1995-06-15 1999-11-30 Introgene B.V. Packaging systems for human recombinant adenovirus to be used in gene therapy
DE19523585A1 (de) * 1995-06-29 1997-01-02 Basf Ag Schlagzähe Formmasse aus Styrolpolymeren
US5773695A (en) * 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
DE19621688A1 (de) * 1996-05-30 1997-12-04 Basf Ag Transparente, nicht-blockende Folie
US6107477A (en) 1996-09-26 2000-08-22 Aurora Biosciences Corporation Non-optimal Kozaks sequences
EP0973866A4 (fr) 1997-03-04 2000-04-19 Baxter Int Lignees cellulaires de complementation de la region e1 d'un adenovirus
US5888809A (en) * 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
DE19832245A1 (de) 1998-07-17 2000-01-20 Heidelberger Druckmasch Ag Verfahren zum Bedienen von Geräten in der Reproduktionstechnik
CN100365127C (zh) 1998-07-21 2008-01-30 密理博公司 具有遍在染色质开放表达元件(ucoe)的聚核苷酸
ES2375266T3 (es) 1998-07-21 2012-02-28 Millipore Corporation Un polinucleótido que comprende un elemento de apertura de cromatina ubicuo (ucoe).
ATE370247T1 (de) * 1998-08-14 2007-09-15 Chromagenics Bv Dns sequenz, verfahren für dessen nachweis und herstellung und ihre benutzung
US6800457B2 (en) * 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6521419B1 (en) * 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
WO2000023606A1 (fr) * 1998-10-22 2000-04-27 Medical College Of Georgia Institute, Inc. Longue terminaison repetee, activateur, et sequences isolantes utilises dans des vecteurs recombinants
JP4210036B2 (ja) 1999-04-15 2009-01-14 クルセル ホランド ベー ヴェー ヒト細胞における組み換え蛋白質の生産
US6391981B1 (en) * 1999-04-23 2002-05-21 Kraton Polymers Us Llc Increased throughput in the manufacture of anionic polymers by reduction in polymer cement viscosity through the addition of metal alkyls
WO2001002553A2 (fr) 1999-06-30 2001-01-11 The Government Of The United States Of America As Represented By The Secretary, Department Of Health And Human Services Sequence et proteine de liaison a l'adn utilisees en tant qu'isolants dotes d'une activite de blocage d'amplificateurs specifiques pour la regulation d'une expression genique
NZ529329A (en) * 1999-08-25 2005-06-24 Immunex Corp Compositions and methods for improved cell culture
WO2001032901A1 (fr) * 1999-11-01 2001-05-10 Chiron Corporation Vecteurs d'expression, systemes de transfection et leur procede d'utilisation
US6558948B1 (en) 1999-11-23 2003-05-06 Stefan Kochanek Permanent amniocytic cell line, its production and use for the production of gene transfer vectors
US20030166042A1 (en) * 2000-02-11 2003-09-04 Millennium Pharmaceuticals, Inc. Novel seven-transmembrane proteins/G-protein coupled receptors
US6586205B1 (en) * 2000-02-11 2003-07-01 Millennium Pharmaceuticals, Inc. 43239 a novel GPCR-like molecule and uses thereof
EP1430124B1 (fr) 2000-09-20 2013-11-06 EMD Millipore Corporation Elements artificiels ubiquistes d'ouverture de la chromatine (ucoe)
WO2002074969A2 (fr) 2001-01-26 2002-09-26 University Of Lausanne Regions se liant a la matrice nucleaire (mar) et procedes d'utilisation de celles-ci
US20020155540A1 (en) * 2001-02-14 2002-10-24 Malla Padidam Method to reduce transcriptional interference between tandem genes
FR2821855B1 (fr) * 2001-03-09 2004-04-02 Cayla Genes synthetiques et plasmides bacteriens depourvus de cpg
TWI324181B (en) * 2001-04-16 2010-05-01 Martek Biosciences Corp Product and process for transformation of thraustochytriales microorganisms
US20040161817A1 (en) 2001-06-04 2004-08-19 Corixa Corporation Compositions and methods for high-level, large-scale production of recombinant proteins
DE60236331D1 (de) * 2001-07-04 2010-06-17 Chromagenics Bv DNS-Sequenzen mit Anti-Repressor-Aktivität
US20040038304A1 (en) * 2002-03-28 2004-02-26 Gala Design, Inc. Antibody libraries
CA2489475C (fr) 2002-06-14 2011-03-29 Chromagenics B.V. Procede permettant la production simultanee de plusieurs proteines; vecteurs et cellules utilises dans ce procede
WO2003106674A2 (fr) * 2002-06-14 2003-12-24 Chromagenics B.V. Moyens et procedes de regulation de l'expression genique
EP1554389A2 (fr) 2002-09-20 2005-07-20 Novo Nordisk A/S Procede de fabrication de proteines recombinantes
ATE524559T1 (de) * 2002-12-18 2011-09-15 Chromagenics Bv Methode zur verbesserung der proteinproduktion
DE60312039T2 (de) * 2002-12-20 2007-11-08 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
CN1863913B (zh) 2003-10-24 2011-01-12 思兰克斯有限公司 通过mar序列的多次转染过程在哺乳动物细胞中实现高效基因转移和表达
JP4805262B2 (ja) * 2004-07-08 2011-11-02 クロマジェニックス ベー ヴェー 核酸の発現を改善するための新規配列
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
ES2384391T3 (es) * 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US7968700B2 (en) * 2006-03-20 2011-06-28 Chromagenics B.V. Expression augmenting DNA fragments, use thereof, and methods for finding thereof

Also Published As

Publication number Publication date
HK1101264A1 (en) 2007-10-12
CA2581422C (fr) 2012-05-22
IL183035A0 (en) 2007-09-20
US20060141577A1 (en) 2006-06-29
AU2005300503A1 (en) 2006-05-11
AU2005300503B2 (en) 2010-12-16
KR101271884B1 (ko) 2013-06-05
MX2007004595A (es) 2007-08-15
KR20070090151A (ko) 2007-09-05
NO20072845L (no) 2007-06-05
WO2006048459A2 (fr) 2006-05-11
NO344469B1 (no) 2019-12-30
JP5291341B2 (ja) 2013-09-18
EP1809750A2 (fr) 2007-07-25
EP1809750B1 (fr) 2012-03-21
JP2008518613A (ja) 2008-06-05
WO2006048459A3 (fr) 2006-08-10
CA2581422A1 (fr) 2006-05-11
EA010863B1 (ru) 2008-12-30
EA200701023A1 (ru) 2007-10-26
BRPI0517380A (pt) 2008-10-07

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