PL1809750T3 - Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach - Google Patents
Selekcja komórki gospodarza eksprymującej białko na wysokich poziomachInfo
- Publication number
- PL1809750T3 PL1809750T3 PL05810935T PL05810935T PL1809750T3 PL 1809750 T3 PL1809750 T3 PL 1809750T3 PL 05810935 T PL05810935 T PL 05810935T PL 05810935 T PL05810935 T PL 05810935T PL 1809750 T3 PL1809750 T3 PL 1809750T3
- Authority
- PL
- Poland
- Prior art keywords
- selection
- host cells
- cells expressing
- high levels
- expressing protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/10—Cells modified by introduction of foreign genetic material
- C12N5/12—Fused cells, e.g. hybridomas
- C12N5/16—Animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/46—Vector systems having a special element relevant for transcription elements influencing chromatin structure, e.g. scaffold/matrix attachment region, methylation free island
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
- C12N2840/206—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES having multiple IRES
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US62630104P | 2004-11-08 | 2004-11-08 | |
EP04105593 | 2004-11-08 | ||
US69661005P | 2005-07-05 | 2005-07-05 | |
EP05810935A EP1809750B1 (fr) | 2004-11-08 | 2005-11-07 | Selection de cellules hotes exprimant une proteine a des niveaux eleves |
PCT/EP2005/055794 WO2006048459A2 (fr) | 2004-11-08 | 2005-11-07 | Selection de cellules hotes exprimant une proteine a des niveaux eleves |
Publications (1)
Publication Number | Publication Date |
---|---|
PL1809750T3 true PL1809750T3 (pl) | 2012-08-31 |
Family
ID=36090897
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
PL05810935T PL1809750T3 (pl) | 2004-11-08 | 2005-11-07 | Selekcja komórki gospodarza eksprymującej białko na wysokich poziomach |
Country Status (14)
Country | Link |
---|---|
US (1) | US20060141577A1 (fr) |
EP (1) | EP1809750B1 (fr) |
JP (1) | JP5291341B2 (fr) |
KR (1) | KR101271884B1 (fr) |
AU (1) | AU2005300503B2 (fr) |
BR (1) | BRPI0517380A (fr) |
CA (1) | CA2581422C (fr) |
EA (1) | EA010863B1 (fr) |
HK (1) | HK1101264A1 (fr) |
IL (1) | IL183035A0 (fr) |
MX (1) | MX2007004595A (fr) |
NO (1) | NO344469B1 (fr) |
PL (1) | PL1809750T3 (fr) |
WO (1) | WO2006048459A2 (fr) |
Families Citing this family (29)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5654442A (en) * | 1989-11-14 | 1997-08-05 | The Perkin-Elmer Corporation | 4,7-dichlorofluorescein dyes as molecular probes |
ATE524559T1 (de) * | 2002-12-18 | 2011-09-15 | Chromagenics Bv | Methode zur verbesserung der proteinproduktion |
DE60312039T2 (de) | 2002-12-20 | 2007-11-08 | Chromagenics B.V. | Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können |
ES2384391T3 (es) | 2004-11-08 | 2012-07-04 | Chromagenics B.V. | Selección de células hospedantes que expresan proteína a altos niveles |
US20060195935A1 (en) * | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
EP2484774A3 (fr) | 2005-07-21 | 2012-11-14 | Abbott Laboratories | Expression de gènes multiples incluant des constructions molles avec des polyprotéines, des pro-polyprotéines et protéolyse |
JP5225107B2 (ja) * | 2006-02-21 | 2013-07-03 | クロマジェニックス ベー ヴェー | 高レベルでタンパク質を発現する宿主細胞の選択 |
US7968700B2 (en) | 2006-03-20 | 2011-06-28 | Chromagenics B.V. | Expression augmenting DNA fragments, use thereof, and methods for finding thereof |
EP2064337B1 (fr) * | 2006-09-20 | 2012-07-18 | Genzyme Corporation | Système à base de facs et de protéine reporteur pour un développement haut débit de protéines thérapeutiques |
US20080274137A1 (en) * | 2007-05-02 | 2008-11-06 | Jean Christophe Francis Audonnet | DNA plasmids having improved expression and stability |
US8653249B2 (en) | 2008-02-27 | 2014-02-18 | Fuso Pharmaceutical Industries, Ltd. | Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof |
WO2010026443A1 (fr) * | 2008-09-08 | 2010-03-11 | Cellectis | Variants de méganucléase clivant une séquence d'adn cible provenant d'un gène de la glutamine synthétase et leurs utilisations |
ES2552325T3 (es) * | 2008-12-22 | 2015-11-27 | National University Corporation Hokkaido University | Vector de expresión para producción a gran escala de proteína derivada de genes foráneos usando células animales, y uso del mismo |
CN102459607B (zh) * | 2009-06-15 | 2014-08-06 | 萨拉基尼克有限公司 | 新的严格的可选择的标志物 |
WO2011113841A1 (fr) * | 2010-03-16 | 2011-09-22 | Robert Steinfeld | Vecteur eucaryote |
EP2582816B1 (fr) | 2010-06-15 | 2017-12-20 | Cellagenics B.V. | Nouvaux éléments intergéniques pour améliorer l'expression de gènes |
CN103180443B (zh) | 2010-09-01 | 2016-01-13 | 萨拉基尼克有限公司 | 用于增强基因表达的来自核糖体蛋白启动子的核酸片段 |
CN113151179A (zh) * | 2012-07-31 | 2021-07-23 | 阿格克斯治疗有限公司 | Hla g修饰的细胞及方法 |
MX2017005788A (es) | 2014-11-04 | 2017-08-02 | Janssen Vaccines & Prevention Bv | Vacunas terapeuticas contra el vph16. |
CA2995740A1 (fr) | 2015-08-20 | 2017-02-23 | Janssen Vaccines & Prevention B.V. | Vaccins therapeutiques contre le vph 18 |
WO2017062722A2 (fr) | 2015-10-09 | 2017-04-13 | Genzyme Corporation | Technologie flare (flow cytometry attenuated reporter expression, expression de reporter atténuée par cytométrie en flux) pour tri en vrac rapide |
US10517944B2 (en) | 2016-05-02 | 2019-12-31 | Janssen Vaccines & Prevention B.V. | Therapeutic HPV vaccine combinations |
TWI795373B (zh) | 2016-10-07 | 2023-03-11 | 美商健臻公司 | 用於生物製劑生產之細胞早期轉染後分離(epic) |
US11781146B2 (en) | 2018-05-24 | 2023-10-10 | National University Corporation Hokkaido University | Vector including a translation-impaired dihydrofolate reductase gene cassette and ubiquitously acting chromatin opening element |
US20210292752A1 (en) * | 2018-08-13 | 2021-09-23 | Spiber Inc. | Method for Isolating or Identifying Cell, and Cell Mass |
US20220033845A1 (en) | 2018-09-24 | 2022-02-03 | Merck Sharp & Dohme Corp. | Expression vectors for eukaryotic expression systems |
EP4247947A1 (fr) | 2020-11-19 | 2023-09-27 | Genzyme Corporation | Système rapporteur multiple à expression rapporteur atténuée par cytométrie de flux et procédés d'utilisation de celui-ci |
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WO2002074969A2 (fr) | 2001-01-26 | 2002-09-26 | University Of Lausanne | Regions se liant a la matrice nucleaire (mar) et procedes d'utilisation de celles-ci |
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EP1554389A2 (fr) | 2002-09-20 | 2005-07-20 | Novo Nordisk A/S | Procede de fabrication de proteines recombinantes |
ATE524559T1 (de) * | 2002-12-18 | 2011-09-15 | Chromagenics Bv | Methode zur verbesserung der proteinproduktion |
DE60312039T2 (de) * | 2002-12-20 | 2007-11-08 | Chromagenics B.V. | Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können |
CN1863913B (zh) | 2003-10-24 | 2011-01-12 | 思兰克斯有限公司 | 通过mar序列的多次转染过程在哺乳动物细胞中实现高效基因转移和表达 |
JP4805262B2 (ja) * | 2004-07-08 | 2011-11-02 | クロマジェニックス ベー ヴェー | 核酸の発現を改善するための新規配列 |
US20060195935A1 (en) * | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US20100136616A1 (en) * | 2004-11-08 | 2010-06-03 | Chromagenics B.V. | Selection of Host Cells Expressing Protein at High Levels |
US8039230B2 (en) * | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
ES2384391T3 (es) * | 2004-11-08 | 2012-07-04 | Chromagenics B.V. | Selección de células hospedantes que expresan proteína a altos niveles |
US8999667B2 (en) * | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US7968700B2 (en) * | 2006-03-20 | 2011-06-28 | Chromagenics B.V. | Expression augmenting DNA fragments, use thereof, and methods for finding thereof |
-
2005
- 2005-11-07 EA EA200701023A patent/EA010863B1/ru not_active IP Right Cessation
- 2005-11-07 EP EP05810935A patent/EP1809750B1/fr active Active
- 2005-11-07 PL PL05810935T patent/PL1809750T3/pl unknown
- 2005-11-07 MX MX2007004595A patent/MX2007004595A/es active IP Right Grant
- 2005-11-07 CA CA2581422A patent/CA2581422C/fr not_active Expired - Fee Related
- 2005-11-07 BR BRPI0517380-9A patent/BRPI0517380A/pt not_active Application Discontinuation
- 2005-11-07 WO PCT/EP2005/055794 patent/WO2006048459A2/fr active Search and Examination
- 2005-11-07 KR KR1020077010362A patent/KR101271884B1/ko active IP Right Grant
- 2005-11-07 AU AU2005300503A patent/AU2005300503B2/en not_active Ceased
- 2005-11-07 JP JP2007539588A patent/JP5291341B2/ja not_active Expired - Fee Related
-
2006
- 2006-02-21 US US11/359,953 patent/US20060141577A1/en not_active Abandoned
-
2007
- 2007-05-07 IL IL183035A patent/IL183035A0/en active IP Right Grant
- 2007-06-05 NO NO20072845A patent/NO344469B1/no not_active IP Right Cessation
- 2007-08-28 HK HK07109398.2A patent/HK1101264A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
HK1101264A1 (en) | 2007-10-12 |
CA2581422C (fr) | 2012-05-22 |
IL183035A0 (en) | 2007-09-20 |
US20060141577A1 (en) | 2006-06-29 |
AU2005300503A1 (en) | 2006-05-11 |
AU2005300503B2 (en) | 2010-12-16 |
KR101271884B1 (ko) | 2013-06-05 |
MX2007004595A (es) | 2007-08-15 |
KR20070090151A (ko) | 2007-09-05 |
NO20072845L (no) | 2007-06-05 |
WO2006048459A2 (fr) | 2006-05-11 |
NO344469B1 (no) | 2019-12-30 |
JP5291341B2 (ja) | 2013-09-18 |
EP1809750A2 (fr) | 2007-07-25 |
EP1809750B1 (fr) | 2012-03-21 |
JP2008518613A (ja) | 2008-06-05 |
WO2006048459A3 (fr) | 2006-08-10 |
CA2581422A1 (fr) | 2006-05-11 |
EA010863B1 (ru) | 2008-12-30 |
EA200701023A1 (ru) | 2007-10-26 |
BRPI0517380A (pt) | 2008-10-07 |
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