ES2552325T3 - Vector de expresión para producción a gran escala de proteína derivada de genes foráneos usando células animales, y uso del mismo - Google Patents

Vector de expresión para producción a gran escala de proteína derivada de genes foráneos usando células animales, y uso del mismo Download PDF

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Publication number
ES2552325T3
ES2552325T3 ES09834881.6T ES09834881T ES2552325T3 ES 2552325 T3 ES2552325 T3 ES 2552325T3 ES 09834881 T ES09834881 T ES 09834881T ES 2552325 T3 ES2552325 T3 ES 2552325T3
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Spain
Prior art keywords
gene cassette
translation
expression vector
codons
codon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
ES09834881.6T
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English (en)
Inventor
Hiroshi Tahara
Yusuke Suzuki
Keiichi Yamamoto
Yuzuru Kitahara
Yasuhiko Suzuki
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hokkaido University NUC
Fuso Pharmaceutical Industries Ltd
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Hokkaido University NUC
Fuso Pharmaceutical Industries Ltd
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Application filed by Hokkaido University NUC, Fuso Pharmaceutical Industries Ltd filed Critical Hokkaido University NUC
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Un vector de expresión para permitir la producción de alto nivel de una proteína derivada de gen foráneo en una célula huésped de mamífero, que comprende: (a) un casete del gen de la dihidrofolato reductasa de traducción alterada (casete del gen DHFR de traducción alterada), cuya expresión se atenúa mediante la alteración de los codones con los codones menos frecuentemente utilizados en un mamífero, en donde los codones han sido alterados con ACG para alanina, CGA para arginina, AAU para asparagina, GAU para ácido aspártico, UGU para cisteína, CAA para glutamina, GAA para ácido glutámico, GGU para glicina, CAU para histidina, UUA para leucina, AAA para lisina, CCA para prolina, UUU para fenilalanina, UCA para serina, ACU para treonina, UAU para tirosina, y/o GUA para valina, y en donde una región de codón alterado en el casete del gen DHFR de traducción alterada es 30% o más de la longitud completa del casete del gen; y (b) un casete de un gen que comprende un sitio de clonación para la integración de un gen foráneo entre un promotor altamente activo transcripcionalmente y una señal de poliadenilación altamente estable.
ES09834881.6T 2008-12-22 2009-12-22 Vector de expresión para producción a gran escala de proteína derivada de genes foráneos usando células animales, y uso del mismo Active ES2552325T3 (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2008325690 2008-12-22
JP2008325690 2008-12-22
PCT/JP2009/071326 WO2010074080A1 (ja) 2008-12-22 2009-12-22 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用

Publications (1)

Publication Number Publication Date
ES2552325T3 true ES2552325T3 (es) 2015-11-27

Family

ID=42287688

Family Applications (1)

Application Number Title Priority Date Filing Date
ES09834881.6T Active ES2552325T3 (es) 2008-12-22 2009-12-22 Vector de expresión para producción a gran escala de proteína derivada de genes foráneos usando células animales, y uso del mismo

Country Status (12)

Country Link
US (1) US9096878B2 (es)
EP (1) EP2385115B1 (es)
JP (1) JP5704753B2 (es)
KR (1) KR101655492B1 (es)
CN (2) CN104342454A (es)
AU (1) AU2009331326B2 (es)
CA (1) CA2748011C (es)
DK (1) DK2385115T3 (es)
ES (1) ES2552325T3 (es)
HK (3) HK1161898A1 (es)
PT (1) PT2385115E (es)
WO (1) WO2010074080A1 (es)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2719108T3 (es) 2011-11-30 2019-07-08 Abbvie Biotechnology Ltd Vectores y células hospedadoras que comprenden un promotor de SV40 modificado para la expresión de proteínas
CN107338267A (zh) * 2016-05-03 2017-11-10 中国科学院深圳先进技术研究院 双顺反子表达载体及其构建方法与应用
BR112019019077A2 (pt) 2017-03-27 2020-04-22 Fuso Pharmaceutical Ind anticorpo anti-pd-l1 para detecção de pd-l1
EP3805396A4 (en) * 2018-05-24 2022-03-23 National University Corporation Hokkaido University NOVEL VECTOR AND USE OF THEM
CN111466337B (zh) * 2020-05-19 2022-04-19 山东大学齐鲁医院 一种腹主动脉瘤动物模型及其构建方法

Family Cites Families (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4713339A (en) 1983-01-19 1987-12-15 Genentech, Inc. Polycistronic expression vector construction
AU572108B2 (en) 1983-01-19 1988-05-05 Genentech Inc. Human tpa production using vectors coding for dhfr protein
NZ210501A (en) 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
JP2648860B2 (ja) 1986-05-08 1997-09-03 イエダ リサーチ アンド デベロップメント コンパニー リミテッド ヒトインターフエロン−β２Ａ及びヒトインターフエロン−β２Ｂ、該インターフエロンをコードする遺伝子を含むベクター、該インターフエロンを産生するセルライン及び該インターフエロンの医薬品としての用途
ATE199023T1 (de) * 1991-03-29 2001-02-15 Genentech Inc Methoden zur selektion von rekombinanten wirtszellen welche hohe mengen von einem gewünschten protein exprimieren
DE4142077A1 (de) 1991-12-19 1993-06-24 Boehringer Mannheim Gmbh Verfahren zur expression rekombinanter antikoerper
JPH0630788A (ja) 1992-07-16 1994-02-08 Otsuka Pharmaceut Co Ltd ヒトインターロイキン−１に対する組換え抗体
US5648267A (en) 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
JP3919830B2 (ja) 1992-11-28 2007-05-30 財団法人化学及血清療法研究所 抗ネコヘルペスウイルス−１組換え抗体および該抗体をコードする遺伝子断片
US5627033A (en) 1995-03-28 1997-05-06 Research & Diagnostics Systems, Inc. Mammalian expression vectors
JP3582965B2 (ja) 1996-08-23 2004-10-27 株式会社イムノ・ジャパン 哺乳動物細胞用発現ベクター
EP2278022A3 (en) * 1999-11-01 2011-05-18 Novartis Vaccines and Diagnostics, Inc. Expression vectors, transfection systems, and method of use thereof
AU2003258388C1 (en) * 2002-09-13 2010-03-18 The University Of Queensland Gene expression system based on codon translation efficiency
KR101149777B1 (ko) * 2002-11-29 2012-06-11 베링거 잉겔하임 파르마 게엠베하 운트 코 카게 신규한 네오마이신 포스포트랜스퍼라제 유전자 및 고생산성 재조합 세포의 선별 방법
CN1771324A (zh) * 2003-02-03 2006-05-10 日本免疫股份有限公司 动物细胞用高表达载体
MX2007004595A (es) * 2004-11-08 2007-08-15 Chromagenics Bv Seleccion de celulas hospederas que expresan proteinas en altas concentraciones.
EP2390326B1 (en) * 2005-03-31 2014-07-09 Fuso Pharmaceutical Industries Ltd. Process of producing human type II collagen
EP1987150B1 (en) 2006-02-21 2011-06-01 ChromaGenics B.V. Selection of host cells expressing protein at high levels
CA2716766A1 (en) 2008-02-27 2009-09-03 Fuso Pharmaceutical Industries, Ltd. Expression vector for mass production of foreign gene-derived protein using animal cell and use thereof
CA2748015C (en) 2008-12-22 2017-02-28 National University Corporation Hokkaido University Protein substance having triple helix structure and manufacturing method therefor

Also Published As

Publication number Publication date
KR20110106392A (ko) 2011-09-28
CA2748011C (en) 2019-02-26
CN102325881A (zh) 2012-01-18
AU2009331326A2 (en) 2011-08-11
DK2385115T3 (en) 2015-11-30
CA2748011A1 (en) 2010-07-01
CN102325881B (zh) 2014-08-20
US20120122083A1 (en) 2012-05-17
EP2385115A4 (en) 2012-08-08
HK1161898A1 (en) 2012-08-10
JPWO2010074080A1 (ja) 2012-06-21
AU2009331326A1 (en) 2011-07-07
AU2009331326B2 (en) 2014-09-18
HK1204651A1 (en) 2015-11-27
KR101655492B1 (ko) 2016-09-07
EP2385115B1 (en) 2015-09-09
US9096878B2 (en) 2015-08-04
JP5704753B2 (ja) 2015-04-22
PT2385115E (pt) 2015-11-24
EP2385115A1 (en) 2011-11-09
CN104342454A (zh) 2015-02-11
HK1163739A1 (en) 2012-09-14
WO2010074080A1 (ja) 2010-07-01

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