ATE511544T1 - Selektion von wirtszellen mit proteinexpression auf hohem niveau - Google Patents
Selektion von wirtszellen mit proteinexpression auf hohem niveauInfo
- Publication number
- ATE511544T1 ATE511544T1 AT07712283T AT07712283T ATE511544T1 AT E511544 T1 ATE511544 T1 AT E511544T1 AT 07712283 T AT07712283 T AT 07712283T AT 07712283 T AT07712283 T AT 07712283T AT E511544 T1 ATE511544 T1 AT E511544T1
- Authority
- AT
- Austria
- Prior art keywords
- polypeptide
- interest
- selectable marker
- host cells
- coding
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2830/00—Vector systems having a special element relevant for transcription
- C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2840/00—Vectors comprising a special translation-regulating system
- C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
- C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US11/359,953 US20060141577A1 (en) | 2004-11-08 | 2006-02-21 | Selection of host cells expressing protein at high levels |
US11/416,490 US20060195935A1 (en) | 2004-11-08 | 2006-05-02 | Selection of host cells expressing protein at high levels |
EP06113354 | 2006-05-02 | ||
PCT/EP2007/051696 WO2007096399A2 (en) | 2006-02-21 | 2007-02-21 | Selection of host cells expressing protein at high levels |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE511544T1 true ATE511544T1 (de) | 2011-06-15 |
Family
ID=38324190
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07712283T ATE511544T1 (de) | 2006-02-21 | 2007-02-21 | Selektion von wirtszellen mit proteinexpression auf hohem niveau |
AT07728440T ATE488593T1 (de) | 2006-05-02 | 2007-04-24 | Selektion von wirtszellen mit proteinexpression auf hohem niveau |
Family Applications After (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT07728440T ATE488593T1 (de) | 2006-05-02 | 2007-04-24 | Selektion von wirtszellen mit proteinexpression auf hohem niveau |
Country Status (14)
Country | Link |
---|---|
EP (2) | EP1987150B1 (de) |
JP (1) | JP5225107B2 (de) |
KR (1) | KR101328300B1 (de) |
CN (2) | CN101389763B (de) |
AT (2) | ATE511544T1 (de) |
AU (1) | AU2007247242B2 (de) |
CA (2) | CA2637271C (de) |
DK (2) | DK1987150T3 (de) |
EA (1) | EA014332B1 (de) |
HK (1) | HK1128490A1 (de) |
PL (1) | PL1987150T3 (de) |
PT (1) | PT1987150E (de) |
SI (1) | SI1987150T1 (de) |
WO (2) | WO2007096399A2 (de) |
Families Citing this family (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
AU2003290453A1 (en) | 2002-12-20 | 2004-07-14 | Chromagenics B.V. | Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors |
US8999667B2 (en) | 2004-11-08 | 2015-04-07 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US20060195935A1 (en) | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
US8039230B2 (en) | 2004-11-08 | 2011-10-18 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
EP1996705B1 (de) | 2006-03-20 | 2011-08-31 | ChromaGenics B.V. | Die expression verstärkende dna-fragmente, deren verwendung und verfahren zum finden dieser |
WO2010074080A1 (ja) | 2008-12-22 | 2010-07-01 | 国立大学法人北海道大学 | 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用 |
MX2011009025A (es) | 2009-02-27 | 2011-09-28 | Novartis Ag | Sistema de vector de expresion que comprende dos marcadores de seleccion. |
US8765370B2 (en) * | 2009-06-11 | 2014-07-01 | Scinopharm Taiwan, Ltd | Inhibition-based high-throughput screen strategy for cell clones |
BRPI1013141A2 (pt) | 2009-06-15 | 2015-10-06 | Cellagenics B V | novos marcadores selecionaveis rigorosos |
WO2011113841A1 (en) * | 2010-03-16 | 2011-09-22 | Robert Steinfeld | Eukaryotic vector |
EP2572706A4 (de) * | 2010-04-21 | 2014-02-26 | Univ Hokkaido Nat Univ Corp | Lipidmembranenstruktur mit nukleinsäure-transportfähigkeit |
CN103038352B (zh) | 2010-06-15 | 2015-12-16 | 萨拉基尼克有限公司 | 用于增强基因表达的新型基因间元件 |
DK2611915T3 (da) | 2010-09-01 | 2015-06-22 | Cellagenics B V | Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression |
ES2689136T3 (es) | 2013-07-31 | 2018-11-08 | Novartis Ag | Nuevos vectores de selección y métodos de selección de células hospedadoras eucariotas |
CN104531699B (zh) * | 2014-10-09 | 2017-03-15 | 河南农业大学 | 一种增强外源基因表达的猪的ucoe调控元件片段 |
CN106754817B (zh) * | 2016-11-03 | 2021-02-05 | 山西省生物研究院有限公司 | 一种重组自分泌运动因子的表达方法 |
DE102017103383A1 (de) * | 2017-02-20 | 2018-08-23 | aReNA-Bio GbR (vertretungsberechtigter Gesellschafter: Dr. Heribert Bohlen, 50733 Köln) | System und Verfahren zur Zelltyp-spezifischen Translation von RNA-Molekülen in Eukaryoten |
CN112513989B (zh) * | 2018-07-30 | 2022-03-22 | 南京金斯瑞生物科技有限公司 | 密码子优化 |
WO2020036181A1 (ja) * | 2018-08-13 | 2020-02-20 | Spiber株式会社 | 細胞を単離又は同定する方法及び細胞集団 |
CN110484563B (zh) * | 2019-07-25 | 2023-04-07 | 新乡医学院 | 哺乳动物细胞组合表达载体、表达系统、制备方法和应用 |
KR20210080071A (ko) * | 2019-12-20 | 2021-06-30 | (주)셀트리온 | 목적 단백질의 고발현을 위한 인트론을 포함하는 발현 카세트 및 이의 이용 |
Family Cites Families (8)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5256568A (en) * | 1990-02-12 | 1993-10-26 | Regeneron Phamaceuticals, Inc. | Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers |
US6319707B1 (en) * | 1991-08-12 | 2001-11-20 | Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington | Cap-independent multicistronic retroviral vectors |
US5648267A (en) * | 1992-11-13 | 1997-07-15 | Idec Pharmaceuticals Corporation | Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same |
WO1996012008A1 (en) * | 1994-10-13 | 1996-04-25 | Merck & Co., Inc. | Synthesis of methylase-resistant genes |
DK1513937T3 (da) * | 2002-06-14 | 2011-05-16 | Chromagenics Bv | Fremgangsmåde til den samtidige produktion af multiple proteiner; vektorer og celler til anvendelse derved |
BRPI0517380A (pt) * | 2004-11-08 | 2008-10-07 | Chromagenics Bv | molécula de dna, cassete de expressão, célula hospedeira, e, métodos para gerar uma célula hospedeira que expressa um polipeptìdeo de interesse e para produzir um polipeptìdeo de interesse |
US20060195935A1 (en) * | 2004-11-08 | 2006-08-31 | Chromagenics B.V. | Selection of host cells expressing protein at high levels |
ES2384391T3 (es) * | 2004-11-08 | 2012-07-04 | Chromagenics B.V. | Selección de células hospedantes que expresan proteína a altos niveles |
-
2007
- 2007-02-21 CN CN2007800062364A patent/CN101389763B/zh not_active Expired - Fee Related
- 2007-02-21 JP JP2008555793A patent/JP5225107B2/ja not_active Expired - Fee Related
- 2007-02-21 EP EP07712283A patent/EP1987150B1/de active Active
- 2007-02-21 SI SI200730698T patent/SI1987150T1/sl unknown
- 2007-02-21 KR KR1020087018437A patent/KR101328300B1/ko active IP Right Grant
- 2007-02-21 EA EA200870279A patent/EA014332B1/ru not_active IP Right Cessation
- 2007-02-21 WO PCT/EP2007/051696 patent/WO2007096399A2/en active Application Filing
- 2007-02-21 PL PL07712283T patent/PL1987150T3/pl unknown
- 2007-02-21 PT PT07712283T patent/PT1987150E/pt unknown
- 2007-02-21 CA CA2637271A patent/CA2637271C/en not_active Expired - Fee Related
- 2007-02-21 DK DK07712283.6T patent/DK1987150T3/da active
- 2007-02-21 AT AT07712283T patent/ATE511544T1/de active
- 2007-04-24 AT AT07728440T patent/ATE488593T1/de not_active IP Right Cessation
- 2007-04-24 CA CA2651088A patent/CA2651088C/en not_active Expired - Fee Related
- 2007-04-24 CN CN2007800158136A patent/CN101437946B/zh not_active Expired - Fee Related
- 2007-04-24 AU AU2007247242A patent/AU2007247242B2/en not_active Ceased
- 2007-04-24 DK DK07728440.4T patent/DK2013349T3/da active
- 2007-04-24 WO PCT/EP2007/053984 patent/WO2007128685A1/en active Application Filing
- 2007-04-24 EP EP07728440A patent/EP2013349B1/de not_active Not-in-force
-
2009
- 2009-08-20 HK HK09107655.2A patent/HK1128490A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
EA014332B1 (ru) | 2010-10-29 |
CN101389763A (zh) | 2009-03-18 |
PT1987150E (pt) | 2011-09-08 |
WO2007096399A2 (en) | 2007-08-30 |
HK1128490A1 (en) | 2009-10-30 |
EA200870279A1 (ru) | 2009-02-27 |
EP1987150A2 (de) | 2008-11-05 |
WO2007128685A1 (en) | 2007-11-15 |
CA2637271C (en) | 2014-12-09 |
AU2007217431A1 (en) | 2007-08-30 |
AU2007247242A1 (en) | 2007-11-15 |
CA2651088A1 (en) | 2007-11-15 |
SI1987150T1 (sl) | 2011-09-30 |
DK2013349T3 (da) | 2011-02-28 |
WO2007096399A3 (en) | 2008-05-02 |
CN101389763B (zh) | 2013-01-23 |
EP1987150B1 (de) | 2011-06-01 |
EP2013349A1 (de) | 2009-01-14 |
CA2651088C (en) | 2016-03-08 |
KR101328300B1 (ko) | 2013-11-14 |
CA2637271A1 (en) | 2007-08-30 |
ATE488593T1 (de) | 2010-12-15 |
JP5225107B2 (ja) | 2013-07-03 |
KR20080105034A (ko) | 2008-12-03 |
CN101437946A (zh) | 2009-05-20 |
CN101437946B (zh) | 2012-07-04 |
JP2009527240A (ja) | 2009-07-30 |
AU2007247242B2 (en) | 2012-03-08 |
PL1987150T3 (pl) | 2011-10-31 |
DK1987150T3 (da) | 2011-09-19 |
EP2013349B1 (de) | 2010-11-17 |
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