DK2611915T3 - Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression - Google Patents

Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression

Info

Publication number
DK2611915T3
DK2611915T3 DK11751675.7T DK11751675T DK2611915T3 DK 2611915 T3 DK2611915 T3 DK 2611915T3 DK 11751675 T DK11751675 T DK 11751675T DK 2611915 T3 DK2611915 T3 DK 2611915T3
Authority
DK
Denmark
Prior art keywords
nucleic acid
gene expression
acid fragments
ribosomal protein
protein promoter
Prior art date
Application number
DK11751675.7T
Other languages
English (en)
Inventor
Arie Pieter Otte
Femke Hoeksema
John Antonius Verhees
Blokland Henricus Johannes Maria Van
Original Assignee
Cellagenics B V
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellagenics B V filed Critical Cellagenics B V
Application granted granted Critical
Publication of DK2611915T3 publication Critical patent/DK2611915T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
DK11751675.7T 2010-09-01 2011-08-31 Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression DK2611915T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US37902510P 2010-09-01 2010-09-01
PCT/NL2011/050593 WO2012030218A1 (en) 2010-09-01 2011-08-31 Nucleic acid fragments from a ribosomal protein promoter for enhancing gene expression

Publications (1)

Publication Number Publication Date
DK2611915T3 true DK2611915T3 (da) 2015-06-22

Family

ID=44543719

Family Applications (1)

Application Number Title Priority Date Filing Date
DK11751675.7T DK2611915T3 (da) 2010-09-01 2011-08-31 Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression

Country Status (7)

Country Link
US (1) US9650630B2 (da)
EP (1) EP2611915B1 (da)
CN (1) CN103180443B (da)
DK (1) DK2611915T3 (da)
IL (1) IL225013A (da)
WO (1) WO2012030218A1 (da)
ZA (1) ZA201301600B (da)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2787080B1 (en) * 2011-11-28 2018-03-21 Daiichi Sankyo Company, Limited Promoter derived from human gene
CA2927862C (en) * 2013-12-30 2024-01-23 Curevac Ag Artificial nucleic acid molecules
EP3666287A1 (en) * 2013-12-31 2020-06-17 ProteoNic Biotechnology IP B.V. Construct and sequence for enhanced gene expression
CN105821077B (zh) * 2015-01-08 2020-02-21 上海迈泰君奥生物技术有限公司 一种新型真核表达系统、其构建方法及应用
GB201603577D0 (en) * 2016-03-01 2016-04-13 Oxford Genetics Ltd Promoter
CN109456993A (zh) * 2018-11-28 2019-03-12 上海安民生物技术有限公司 含cag启动子的白蛋白表达载体

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965196A (en) 1983-01-19 1990-10-23 Genentech, Inc. Polycistronic expression vector construction
US5561053A (en) 1994-08-05 1996-10-01 Genentech, Inc. Method for selecting high-expressing host cells
GB9517263D0 (en) 1995-08-23 1995-10-25 Cancer Res Campaign Tech Expression systems
US6110707A (en) 1996-01-19 2000-08-29 Board Of Regents, The University Of Texas System Recombinant expression of proteins from secretory cell lines
EP1228237A1 (en) 1999-11-01 2002-08-07 Chiron Corporation Expression vectors, transfection systems, and method of use thereof
US6908751B2 (en) 2000-04-26 2005-06-21 Zymogenetics, Inc. Methods for enhancing the expression of a protein of interest by recombinant host cells
AU2001273738B2 (en) 2000-06-13 2004-09-02 The University Of Queensland Expression modulating sequences
ATE466942T1 (de) 2001-07-04 2010-05-15 Chromagenics Bv Dns-sequenzen mit anti-repressor aktivität
EP1513936A2 (en) 2002-06-14 2005-03-16 Chromagenics B.V. Use of repression blocking sequences in methods for enhancing gene expression
DK1513937T3 (da) 2002-06-14 2011-05-16 Chromagenics Bv Fremgangsmåde til den samtidige produktion af multiple proteiner; vektorer og celler til anvendelse derved
US7825232B2 (en) 2004-07-08 2010-11-02 Chromagenics B.V. Sequence for improving expression of nucleic acid
ES2384391T3 (es) 2004-11-08 2012-07-04 Chromagenics B.V. Selección de células hospedantes que expresan proteína a altos niveles
KR101271884B1 (ko) 2004-11-08 2013-06-05 크로마제닉스 비.브이. 고수준으로 단백질을 발현하는 숙주세포의 선택
GB0509965D0 (en) 2005-05-17 2005-06-22 Ml Lab Plc Improved expression elements
EP1760092A1 (en) 2005-08-26 2007-03-07 Applied Research Systems ARS Holding N.V. System for screening cells for high expression of a protein of interest
PT1987150E (pt) * 2006-02-21 2011-09-08 Chromagenics Bv Selecção de células hospedeiras expressando níveis elevados de proteína
WO2010147464A1 (en) 2009-06-15 2010-12-23 Cellagenics B.V. Use of a cysteine synthesizing enzyme as selectable marker

Also Published As

Publication number Publication date
WO2012030218A1 (en) 2012-03-08
US9650630B2 (en) 2017-05-16
US20130236956A1 (en) 2013-09-12
ZA201301600B (en) 2014-08-27
CN103180443B (zh) 2016-01-13
CN103180443A (zh) 2013-06-26
EP2611915B1 (en) 2015-04-15
EP2611915A1 (en) 2013-07-10
IL225013A (en) 2016-08-31

Similar Documents

Publication Publication Date Title
DK3623481T3 (da) Sammensætninger til nukleinsyresekventering
HK1200869A1 (en) Nucleic acid fragment binding to target protein
HK1201077A1 (en) Sequencing small amounts of complex nucleic acids
BR112014009066A2 (pt) dna triciclo-fosforotioato
IL232512A0 (en) Comparative parallel analysis of high-throughput sequencing data
GB2499340B (en) Methods for sequencing nucleic acids
EP2917372A4 (en) NUCLEIC ACID SEQUENCING USING LABELS
DK2699698T4 (da) Oscillerende amplifikations-reaktion for nukleinsyrer
EP2707381A4 (en) NUCLEAR ACID EXTRACTION FROM HETEROGENIC BIOLOGICAL MATERIALS
EP2673380A4 (en) ANALYSIS OF NUCLEIC ACIDS
GB2494239B (en) Nucleic acid amplification
EP2872523A4 (en) NUCLEIC ACID PURIFICATION OF A MICROORGANISM FROM HOST SAMPLES
PL2623507T3 (pl) Pochodna kwasu morfolinonukleinowego
EP2729182A4 (en) NUCLEIC COMPLEX
EP2705162A4 (en) METHOD FOR DETECTING NUCLEIC ACID SEQUENCE VARIANTS
EP2764112A4 (en) DIRECT NUCLEIC ACID ANALYSIS
GB201119390D0 (en) Novel methods for determining nucleotide sequence repeats
DK2611915T3 (da) Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression
ZA201209260B (en) Novel intergenic elements for enhancing gene expression
HK1188608A1 (en) Dna expression construct dna
EP2774989A4 (en) DOUBLE-STRUCTURED NUCLEIC ACID MOLECULE FOR GENE EXPRESSION CONTROL
EP2700414A4 (en) SELF-GELATINABLE NUCLEIC ACID
EP2771462A4 (en) PURIFICATION OF A NUCLEIC ACID
EP2682462A4 (en) (Nucleic acid) polysaccharide COMPLEX
EP2794904A4 (en) AMPLIFICATION OF A SEQUENCE FROM A RIBONUCLEIC ACID