ATE488593T1 - Selektion von wirtszellen mit proteinexpression auf hohem niveau - Google Patents

Selektion von wirtszellen mit proteinexpression auf hohem niveau

Info

Publication number
ATE488593T1
ATE488593T1 AT07728440T AT07728440T ATE488593T1 AT E488593 T1 ATE488593 T1 AT E488593T1 AT 07728440 T AT07728440 T AT 07728440T AT 07728440 T AT07728440 T AT 07728440T AT E488593 T1 ATE488593 T1 AT E488593T1
Authority
AT
Austria
Prior art keywords
polypeptide
interest
selectable marker
host cells
coding
Prior art date
Application number
AT07728440T
Other languages
English (en)
Inventor
Arie Otte
Blokland Henricus Van
Theodorus Kwaks
Richard Sewalt
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US11/416,490 external-priority patent/US20060195935A1/en
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Application granted granted Critical
Publication of ATE488593T1 publication Critical patent/ATE488593T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/40—Vector systems having a special element relevant for transcription being an insulator
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C12N2840/203—Vectors comprising a special translation-regulating system translation of more than one cistron having an IRES

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
AT07728440T 2006-05-02 2007-04-24 Selektion von wirtszellen mit proteinexpression auf hohem niveau ATE488593T1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US11/416,490 US20060195935A1 (en) 2004-11-08 2006-05-02 Selection of host cells expressing protein at high levels
EP06113354 2006-05-02
PCT/EP2007/053984 WO2007128685A1 (en) 2006-05-02 2007-04-24 Selection of host cells expressing protein at high levels

Publications (1)

Publication Number Publication Date
ATE488593T1 true ATE488593T1 (de) 2010-12-15

Family

ID=38324190

Family Applications (2)

Application Number Title Priority Date Filing Date
AT07712283T ATE511544T1 (de) 2006-02-21 2007-02-21 Selektion von wirtszellen mit proteinexpression auf hohem niveau
AT07728440T ATE488593T1 (de) 2006-05-02 2007-04-24 Selektion von wirtszellen mit proteinexpression auf hohem niveau

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT07712283T ATE511544T1 (de) 2006-02-21 2007-02-21 Selektion von wirtszellen mit proteinexpression auf hohem niveau

Country Status (14)

Country Link
EP (2) EP1987150B1 (de)
JP (1) JP5225107B2 (de)
KR (1) KR101328300B1 (de)
CN (2) CN101389763B (de)
AT (2) ATE511544T1 (de)
AU (1) AU2007247242B2 (de)
CA (2) CA2637271C (de)
DK (2) DK1987150T3 (de)
EA (1) EA014332B1 (de)
HK (1) HK1128490A1 (de)
PL (1) PL1987150T3 (de)
PT (1) PT1987150E (de)
SI (1) SI1987150T1 (de)
WO (2) WO2007096399A2 (de)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1572994B1 (de) 2002-12-20 2007-02-21 Chromagenics B.V. Mittel und methoden zur produktion eines proteins durch chromatin-öffner, die chromatin zugänglicher für transkriptionsfaktoren machen können
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1996705B1 (de) 2006-03-20 2011-08-31 ChromaGenics B.V. Die expression verstärkende dna-fragmente, deren verwendung und verfahren zum finden dieser
AU2009331326B2 (en) 2008-12-22 2014-09-18 Fuso Pharmaceutical Industries, Ltd. Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof
DK2401377T3 (en) 2009-02-27 2016-08-29 Novartis Ag Expression vector system comprising two selection markers
US8765370B2 (en) * 2009-06-11 2014-07-01 Scinopharm Taiwan, Ltd Inhibition-based high-throughput screen strategy for cell clones
WO2010147464A1 (en) 2009-06-15 2010-12-23 Cellagenics B.V. Use of a cysteine synthesizing enzyme as selectable marker
WO2011113841A1 (en) * 2010-03-16 2011-09-22 Robert Steinfeld Eukaryotic vector
JP5794541B2 (ja) * 2010-04-21 2015-10-14 国立大学法人北海道大学 核内移行性を有する脂質膜構造体
CN103038352B (zh) 2010-06-15 2015-12-16 萨拉基尼克有限公司 用于增强基因表达的新型基因间元件
DK2611915T3 (da) 2010-09-01 2015-06-22 Cellagenics B V Nukleinsyrefragmenter fra en ribosomal proteinpromoter til forstærkning af genekspression
DK3027646T3 (en) 2013-07-31 2018-09-24 Novartis Ag New selection vectors and methods for selecting eukaryotic host cells
CN104531699B (zh) * 2014-10-09 2017-03-15 河南农业大学 一种增强外源基因表达的猪的ucoe调控元件片段
CN106754817B (zh) * 2016-11-03 2021-02-05 山西省生物研究院有限公司 一种重组自分泌运动因子的表达方法
DE102017103383A1 (de) * 2017-02-20 2018-08-23 aReNA-Bio GbR (vertretungsberechtigter Gesellschafter: Dr. Heribert Bohlen, 50733 Köln) System und Verfahren zur Zelltyp-spezifischen Translation von RNA-Molekülen in Eukaryoten
EP3830830A4 (de) * 2018-07-30 2022-05-11 Nanjing GenScript Biotech Co., Ltd. Codonoptimierung
WO2020036181A1 (ja) * 2018-08-13 2020-02-20 Ｓｐｉｂｅｒ株式会社 細胞を単離又は同定する方法及び細胞集団
CN110484563B (zh) * 2019-07-25 2023-04-07 新乡医学院 哺乳动物细胞组合表达载体、表达系统、制备方法和应用
KR20210080071A (ko) * 2019-12-20 2021-06-30 (주)셀트리온 목적 단백질의 고발현을 위한 인트론을 포함하는 발현 카세트 및 이의 이용

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5256568A (en) * 1990-02-12 1993-10-26 Regeneron Phamaceuticals, Inc. Vectors and transformed most cells for recombinant protein production with reduced expression of selectable markers
US6319707B1 (en) 1991-08-12 2001-11-20 Fred Hutchinson Cancer Research Center Board Regents Of The University Of Washington Cap-independent multicistronic retroviral vectors
US5648267A (en) * 1992-11-13 1997-07-15 Idec Pharmaceuticals Corporation Impaired dominant selectable marker sequence and intronic insertion strategies for enhancement of expression of gene product and expression vector systems comprising same
WO1996012008A1 (en) * 1994-10-13 1996-04-25 Merck & Co., Inc. Synthesis of methylase-resistant genes
ES2362273T3 (es) * 2002-06-14 2011-06-30 Chromagenics B.V. Método para la producción simultánea de múltiples proteínas; vectores y células para su uso en el mismo.
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
SI1809750T1 (sl) * 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
JP5291341B2 (ja) * 2004-11-08 2013-09-18 クロマジェニックス ベー ヴェー タンパク質を高レベルで発現する宿主細胞の選定

Also Published As

Publication number Publication date
HK1128490A1 (en) 2009-10-30
AU2007247242A1 (en) 2007-11-15
CA2651088A1 (en) 2007-11-15
AU2007247242B2 (en) 2012-03-08
WO2007096399A2 (en) 2007-08-30
CN101437946B (zh) 2012-07-04
EP1987150B1 (de) 2011-06-01
CA2651088C (en) 2016-03-08
PL1987150T3 (pl) 2011-10-31
WO2007096399A3 (en) 2008-05-02
CA2637271C (en) 2014-12-09
EP2013349B1 (de) 2010-11-17
EA200870279A1 (ru) 2009-02-27
JP2009527240A (ja) 2009-07-30
CN101437946A (zh) 2009-05-20
EA014332B1 (ru) 2010-10-29
WO2007128685A1 (en) 2007-11-15
CN101389763B (zh) 2013-01-23
CA2637271A1 (en) 2007-08-30
EP2013349A1 (de) 2009-01-14
KR20080105034A (ko) 2008-12-03
CN101389763A (zh) 2009-03-18
PT1987150E (pt) 2011-09-08
AU2007217431A1 (en) 2007-08-30
KR101328300B1 (ko) 2013-11-14
DK1987150T3 (da) 2011-09-19
DK2013349T3 (da) 2011-02-28
EP1987150A2 (de) 2008-11-05
JP5225107B2 (ja) 2013-07-03
SI1987150T1 (sl) 2011-09-30
ATE511544T1 (de) 2011-06-15

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