ATE524559T1 - Methode zur verbesserung der proteinproduktion - Google Patents

Methode zur verbesserung der proteinproduktion

Info

Publication number
ATE524559T1
ATE524559T1 AT03779044T AT03779044T ATE524559T1 AT E524559 T1 ATE524559 T1 AT E524559T1 AT 03779044 T AT03779044 T AT 03779044T AT 03779044 T AT03779044 T AT 03779044T AT E524559 T1 ATE524559 T1 AT E524559T1
Authority
AT
Austria
Prior art keywords
protein
sequence
cell
trap
protein expression
Prior art date
Application number
AT03779044T
Other languages
English (en)
Inventor
Arie Otte
Blokland Henricus Van
Original Assignee
Chromagenics Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chromagenics Bv filed Critical Chromagenics Bv
Application granted granted Critical
Publication of ATE524559T1 publication Critical patent/ATE524559T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT03779044T 2002-12-18 2003-12-02 Methode zur verbesserung der proteinproduktion ATE524559T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP02080347 2002-12-18
PCT/NL2003/000850 WO2004055215A1 (en) 2002-12-18 2003-12-02 A method for improving protein production

Publications (1)

Publication Number Publication Date
ATE524559T1 true ATE524559T1 (de) 2011-09-15

Family

ID=32524041

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03779044T ATE524559T1 (de) 2002-12-18 2003-12-02 Methode zur verbesserung der proteinproduktion

Country Status (9)

Country Link
US (2) US7413896B2 (de)
EP (1) EP1573058B1 (de)
AT (1) ATE524559T1 (de)
AU (1) AU2003285823B2 (de)
CA (1) CA2510179C (de)
DK (1) DK1573058T3 (de)
ES (1) ES2373549T3 (de)
NZ (1) NZ540471A (de)
WO (1) WO2004055215A1 (de)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE466941T1 (de) 2001-07-04 2010-05-15 Chromagenics Bv Dns-sequenzen mit anti-repressor-aktivität
EP1513936A2 (de) 2002-06-14 2005-03-16 Chromagenics B.V. Verwendung von repression hemmende sequenzen in verfahren zur genexpression verbesserung
NZ537073A (en) 2002-06-14 2006-08-31 Chromagenics B A method for simultaneous production of multiple proteins; vectors and cells for use therein
ATE524559T1 (de) 2002-12-18 2011-09-15 Chromagenics Bv Methode zur verbesserung der proteinproduktion
WO2004056986A2 (en) 2002-12-20 2004-07-08 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
US8039230B2 (en) 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
US8999667B2 (en) 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
SI1809750T1 (sl) 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
JP5291341B2 (ja) * 2004-11-08 2013-09-18 クロマジェニックス ベー ヴェー タンパク質を高レベルで発現する宿主細胞の選定
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US7732342B2 (en) * 2005-05-26 2010-06-08 Applied Materials, Inc. Method to increase the compressive stress of PECVD silicon nitride films
US7566655B2 (en) * 2005-05-26 2009-07-28 Applied Materials, Inc. Integration process for fabricating stressed transistor structure
SG164383A1 (en) 2005-07-21 2010-09-29 Abbott Lab Multiple gene expression including sorf constructs and methods with polyproteins, pro-proteins, and proteolysis
US20080293086A1 (en) * 2006-09-18 2008-11-27 Cobalt Technologies, Inc. A Delaware Corporation Real time monitoring of microbial enzymatic pathways
US20090081715A1 (en) * 2007-09-07 2009-03-26 Cobalt Technologies, Inc., A Delaware Corporation Engineered Light-Emitting Reporter Genes
CA2721076A1 (en) 2008-04-09 2009-10-15 Cobalt Technologies, Inc. Immobilized product tolerant microorganisms
MX2011013974A (es) 2009-06-26 2012-04-30 Cobalt Technologies Inc Sistema y proceso integrados para la produccion de bioproductos.
RU2012122240A (ru) * 2009-10-30 2013-12-10 Эбботт Лэборетриз Конструкции sorf и экспрессия нескольких генов
AU2014310932B2 (en) * 2013-08-21 2019-06-06 CureVac SE Composition and vaccine for treating lung cancer

Family Cites Families (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5610053A (en) 1993-04-07 1997-03-11 The United States Of America As Represented By The Department Of Health And Human Services DNA sequence which acts as a chromatin insulator element to protect expressed genes from cis-acting regulatory sequences in mammalian cells
US5631158A (en) * 1993-10-25 1997-05-20 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
US5972605A (en) 1994-07-07 1999-10-26 Geron Corporation Assays for regulators of mammalian telomerase expression
US5773695A (en) 1996-01-26 1998-06-30 North Carolina State University Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
CA2333852C (en) 1998-07-21 2007-05-29 Cobra Therapeutics Limited A polynucleotide comprising a ubiquitous chromatin opening element (ucoe)
AU5450599A (en) 1998-08-14 2000-03-06 Stichting Voor De Technische Wetenschappen Method of detecting a dna sequence, a dna sequence, a method of making a dna construct and the use thereof
US6800457B2 (en) 1998-09-22 2004-10-05 Bristol-Myers Squibb Company Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
US6521419B1 (en) 1998-09-22 2003-02-18 Kanakaraju Koduri Expression vectors containing hot spot for increased recombinant protein expression in transfected cells
AU1128400A (en) 1998-10-22 2000-05-08 Medical College Of Georgia Institute, Inc. Long terminal repeat, enhancer, and insulator sequences for use in recombinant vectors
AU2001238178A1 (en) 2000-02-11 2001-08-20 Millennium Pharmaceuticals, Inc. Seven-transmembrane proteins/g-protein coupled receptors
US6586205B1 (en) 2000-02-11 2003-07-01 Millennium Pharmaceuticals, Inc. 43239 a novel GPCR-like molecule and uses thereof
AU2001290067B2 (en) 2000-09-20 2007-04-05 Emd Millipore Corporation Artificial ubiquitous chromatin opening elements (UCOE)
US6787321B1 (en) * 2000-10-13 2004-09-07 The Regents Of The University Of California Mammalian two-hybrid system for screening for modulators of the accumulation of metabolic products
WO2002068627A2 (en) 2001-02-23 2002-09-06 Novartis Ag Vector constucts
EP1273666A1 (de) 2001-07-04 2003-01-08 Chromagenics B.V. Verfahren zur Selektion einer transkription-modulatierenden DNA-Sequenz unter Verwendung einem Vektor mit einem transkriptions-repressions-aktivem Element
ATE466941T1 (de) 2001-07-04 2010-05-15 Chromagenics Bv Dns-sequenzen mit anti-repressor-aktivität
ITMI20012110A1 (it) * 2001-10-12 2003-04-12 Keryos Spa Vettori multi-cistronici utilizzabili in protocolli di trsferimento genico
NZ537073A (en) * 2002-06-14 2006-08-31 Chromagenics B A method for simultaneous production of multiple proteins; vectors and cells for use therein
ATE524559T1 (de) * 2002-12-18 2011-09-15 Chromagenics Bv Methode zur verbesserung der proteinproduktion
WO2004056986A2 (en) * 2002-12-20 2004-07-08 Chromagenics B.V. Means and methods for producing a protein through chromatin openers that are capable of rendering chromatin more accessible to transcription factors
SI1809750T1 (sl) * 2004-11-08 2012-08-31 Chromagenics Bv Izbira gostiteljskih celic, ki imajo visok nivo izraĹľanja proteina
US20100136616A1 (en) * 2004-11-08 2010-06-03 Chromagenics B.V. Selection of Host Cells Expressing Protein at High Levels
US8999667B2 (en) * 2004-11-08 2015-04-07 Chromagenics B.V. Selection of host cells expressing protein at high levels
US20060195935A1 (en) * 2004-11-08 2006-08-31 Chromagenics B.V. Selection of host cells expressing protein at high levels
JP5291341B2 (ja) * 2004-11-08 2013-09-18 クロマジェニックス ベー ヴェー タンパク質を高レベルで発現する宿主細胞の選定
US8039230B2 (en) * 2004-11-08 2011-10-18 Chromagenics B.V. Selection of host cells expressing protein at high levels
EP1996705B1 (de) * 2006-03-20 2011-08-31 ChromaGenics B.V. Die expression verstärkende dna-fragmente, deren verwendung und verfahren zum finden dieser

Also Published As

Publication number Publication date
ES2373549T3 (es) 2012-02-06
EP1573058B1 (de) 2011-09-14
US8173422B2 (en) 2012-05-08
US20090011468A1 (en) 2009-01-08
CA2510179A1 (en) 2004-07-01
US20060010506A1 (en) 2006-01-12
AU2003285823B2 (en) 2008-06-19
EP1573058A1 (de) 2005-09-14
CA2510179C (en) 2013-04-23
AU2003285823A1 (en) 2004-07-09
NZ540471A (en) 2008-07-31
WO2004055215A1 (en) 2004-07-01
US7413896B2 (en) 2008-08-19
DK1573058T3 (da) 2012-01-16

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