PL1735440T3 - Reagenty i sposoby izolacji oczyszczonego RNA - Google Patents

Reagenty i sposoby izolacji oczyszczonego RNA

Info

Publication number
PL1735440T3
PL1735440T3 PL05735516T PL05735516T PL1735440T3 PL 1735440 T3 PL1735440 T3 PL 1735440T3 PL 05735516 T PL05735516 T PL 05735516T PL 05735516 T PL05735516 T PL 05735516T PL 1735440 T3 PL1735440 T3 PL 1735440T3
Authority
PL
Poland
Prior art keywords
reagents
isolation
methods
purified rna
rna
Prior art date
Application number
PL05735516T
Other languages
English (en)
Inventor
Piotr Chomczynski
Original Assignee
Piotr Chomczynski
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34965664&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=PL1735440(T3) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Piotr Chomczynski filed Critical Piotr Chomczynski
Publication of PL1735440T3 publication Critical patent/PL1735440T3/pl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
PL05735516T 2004-04-16 2005-04-14 Reagenty i sposoby izolacji oczyszczonego RNA PL1735440T3 (pl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US10/826,113 US7794932B2 (en) 2004-04-16 2004-04-16 Reagents and methods for isolation of purified RNA
PCT/US2005/012814 WO2005103252A1 (en) 2004-04-16 2005-04-14 Reagents and methods for isolation of purified rna
EP05735516A EP1735440B1 (en) 2004-04-16 2005-04-14 Reagents and methods for isolation of purified rna

Publications (1)

Publication Number Publication Date
PL1735440T3 true PL1735440T3 (pl) 2013-07-31

Family

ID=34965664

Family Applications (2)

Application Number Title Priority Date Filing Date
PL05735516T PL1735440T3 (pl) 2004-04-16 2005-04-14 Reagenty i sposoby izolacji oczyszczonego RNA
PL11001814T PL2322613T5 (pl) 2004-04-16 2005-04-14 Reagenty i sposoby izolacji oczyszczonego RNA

Family Applications After (1)

Application Number Title Priority Date Filing Date
PL11001814T PL2322613T5 (pl) 2004-04-16 2005-04-14 Reagenty i sposoby izolacji oczyszczonego RNA

Country Status (8)

Country Link
US (2) US7794932B2 (pl)
EP (2) EP2322613B2 (pl)
JP (2) JP5247143B2 (pl)
KR (1) KR101218469B1 (pl)
CN (2) CN101979539B (pl)
ES (1) ES2452874T5 (pl)
PL (2) PL1735440T3 (pl)
WO (1) WO2005103252A1 (pl)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7794932B2 (en) * 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
US20070202511A1 (en) 2006-02-28 2007-08-30 Sigma-Aldrich Co. Methods and compositions for the rapid isolation of small RNA molecules
US20080044851A1 (en) * 2006-06-02 2008-02-21 Epicentre Technologies Compositions and methods for removal of DNA from a sample
TWI393776B (zh) * 2008-08-29 2013-04-21 Nat Science And Technology Dev Agency 蛋白提取緩衝液及使用該緩衝液從微生物中提取蛋白的方法
WO2010064628A1 (ja) * 2008-12-05 2010-06-10 オリンパス株式会社 核酸含有試料の調製方法、試料調製用溶液、及び核酸の解析方法
WO2010070657A1 (en) * 2008-12-15 2010-06-24 Council Of Scientific & Industrial Research A composition for the removal of colours and inhibitors from plant tissues to isolate rna
WO2011110644A1 (en) * 2010-03-11 2011-09-15 National University Of Ireland, Galway Detection and quantification of micrornas in the circulation and the use of circulating micrornas as biomarkers for cancer
DK2607482T3 (en) 2010-08-18 2017-07-03 Toray Industries Solution for extraction of RNA
EP2426201A1 (en) 2010-09-06 2012-03-07 Qiagen GmbH Method of isolating purified RNA with reduced DNA contaminations
US9051563B2 (en) * 2011-01-14 2015-06-09 Zymo Research Corporation Nucleic acid purification
US9890431B2 (en) 2011-11-15 2018-02-13 Université Libre de Bruxelles Streptococcus pneumoniae detection in blood
US9416357B2 (en) 2011-11-16 2016-08-16 University Of Georgia Research Foundation, Inc. Method for isolating total RNA from cells
CN102505012A (zh) * 2011-12-30 2012-06-20 珠海出入境检验检疫局检验检疫技术中心 一种用于提取动物病毒rna的裂解液
EP2770056B1 (en) * 2012-07-18 2017-05-31 Zymo Research Corporation Nucleic acid purification
CN103571824B (zh) * 2012-08-09 2016-04-13 财团法人工业技术研究院 用于分离核酸的组合物及方法
CN103571825B (zh) * 2012-08-09 2016-03-16 财团法人工业技术研究院 用于生物样本处理之组合物及使用其之核酸扩增方法
CN102888396B (zh) * 2012-09-06 2014-06-04 中国热带农业科学院橡胶研究所 一种分离植物低分子量rna的方法
CN102876663B (zh) * 2012-10-10 2014-07-30 北京百泰克生物技术有限公司 一种从液体样本中快速提取总rna的试剂及方法
US9828600B2 (en) 2013-09-20 2017-11-28 University Of Massachusetts Compositions and methods for constructing cDNA libraries that allow for mapping the 5′ and 3′ ends of RNAs
WO2015164773A1 (en) 2014-04-25 2015-10-29 Shire Human Genetic Therapies, Inc. Methods for purification of messenger rna
CN105385681B (zh) * 2015-12-29 2018-07-17 中国海洋大学 一种用于鲆鲽鱼类血液rna提取的裂解液
WO2017167716A1 (en) 2016-03-30 2017-10-05 Life Technologies As Improved methods and compositions for nucleic acid isolation
CN107058297B (zh) * 2017-06-05 2018-01-16 陈礼平 一种离液剂以及使用该离液剂提取基因组dna的方法
CN107475243B (zh) * 2017-08-19 2021-03-23 皖南医学院 一种改良酚抽提总rna的方法
CN107523564A (zh) * 2017-10-16 2017-12-29 皖南医学院 一种简单经济钉螺组织总rna的提取方法
US20200347380A1 (en) * 2017-12-06 2020-11-05 Entopsis, LLC Separation Device, and Method of Use, to Remove PCR Inhibitors from Whole Blood and Serum Samples
DE102019108087A1 (de) 2019-03-28 2020-10-01 Axagarius Gmbh & Co. Kg Verfahren zur Isolierung von Nukleinsäuren aus Probematerialien
CN110804609A (zh) * 2019-09-29 2020-02-18 杭州联科生物技术股份有限公司 一种全血rna快速裂解液及应用
CN112522250B (zh) * 2020-12-10 2023-08-22 聊城大学 一种核酸纯化辅助剂及使用方法
CN112662739A (zh) * 2021-01-04 2021-04-16 深圳海普洛斯医学检验实验室 一种用于ngs平台的尿液外泌体rna提取和文库构建方法
CN114573483B (zh) * 2022-03-16 2022-11-01 湖南师范大学 一种疏水的磁性离子液体及其制备方法和应用
CN116656671B (zh) * 2023-07-27 2023-11-21 南京诺唯赞生物科技股份有限公司 一种rna提取方法

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US3163638A (en) * 1960-12-28 1964-12-29 Toyo Boseki Extraction of ribonucleic acid
US4843155A (en) * 1987-11-19 1989-06-27 Piotr Chomczynski Product and process for isolating RNA
US5346994A (en) † 1992-01-28 1994-09-13 Piotr Chomczynski Shelf-stable product and process for isolating RNA, DNA and proteins
US5386024A (en) * 1993-02-10 1995-01-31 Gen-Probe Incorporated Method to prepare nucleic acids from a biological sample using low pH and acid protease
US5637687A (en) † 1993-08-31 1997-06-10 Wiggins; James C. Methods and compositions for isolating nucleic acids
DE69534886T2 (de) 1994-04-14 2006-10-26 The Rockefeller University Verfahren zur Isolierung von Zellkomponenten
US5777099A (en) † 1995-02-24 1998-07-07 Biotecx Laboratories, Inc. RNA separation
US5945515A (en) * 1995-07-31 1999-08-31 Chomczynski; Piotr Product and process for isolating DNA, RNA and proteins
US5973137A (en) * 1996-02-13 1999-10-26 Gentra Systems, Inc. Low pH RNA isolation reagents, method, and kit
JPH09327291A (ja) † 1996-06-11 1997-12-22 Toyobo Co Ltd Ｒｎａの抽出精製方法
US5981235A (en) 1996-07-29 1999-11-09 Promega Corporation Methods for isolating nucleic acids using alkaline protease
EP1005479A4 (en) 1997-04-10 2002-12-18 Life Technologies Inc RNS INSULATION REAGENT AND METHOD
CN1220995A (zh) * 1997-12-23 1999-06-30 陈铁骅 总rna提取试剂及其制造方法以及提取总rna的方法
US6248535B1 (en) * 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
GB2363381B (en) 2000-06-12 2004-11-24 Cambridge Molecular Tech Solution
JP2004329176A (ja) * 2003-05-12 2004-11-25 Plant Genome Center Co Ltd Ｒｎａの単離方法
US7794932B2 (en) * 2004-04-16 2010-09-14 Piotr Chomczynski Reagents and methods for isolation of purified RNA
WO2006011157A2 (en) 2004-06-18 2006-02-02 Lupin Limited Methods for the preparation of aryl piperazinyl-heterocyclic compounds

Also Published As

Publication number Publication date
CN1997740A (zh) 2007-07-11
US20050233333A1 (en) 2005-10-20
PL2322613T3 (pl) 2014-05-30
KR101218469B1 (ko) 2013-01-04
CN1997740B (zh) 2011-12-07
EP2322613B1 (en) 2013-10-16
US7794932B2 (en) 2010-09-14
JP2007532140A (ja) 2007-11-15
CN101979539B (zh) 2014-07-23
ES2452874T5 (es) 2017-06-05
ES2452874T3 (es) 2014-04-03
EP1735440B1 (en) 2013-01-16
EP2322613B2 (en) 2016-11-09
WO2005103252A1 (en) 2005-11-03
JP2011152142A (ja) 2011-08-11
US8367817B2 (en) 2013-02-05
US20080057560A1 (en) 2008-03-06
JP5247143B2 (ja) 2013-07-24
PL2322613T5 (pl) 2017-08-31
JP5572578B2 (ja) 2014-08-13
EP1735440A1 (en) 2006-12-27
KR20070042912A (ko) 2007-04-24
CN101979539A (zh) 2011-02-23
EP2322613A1 (en) 2011-05-18

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