NZ520701A - Serum free cultivation of primate embryonic stem cells with fibroblast growth factor supplied seperately from fibroblast feeder layers - Google Patents
Serum free cultivation of primate embryonic stem cells with fibroblast growth factor supplied seperately from fibroblast feeder layersInfo
- Publication number
- NZ520701A NZ520701A NZ520701A NZ52070101A NZ520701A NZ 520701 A NZ520701 A NZ 520701A NZ 520701 A NZ520701 A NZ 520701A NZ 52070101 A NZ52070101 A NZ 52070101A NZ 520701 A NZ520701 A NZ 520701A
- Authority
- NZ
- New Zealand
- Prior art keywords
- stem cells
- culture
- embryonic stem
- growth factor
- culturing
- Prior art date
Links
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- 102000018233 Fibroblast Growth Factor Human genes 0.000 title claims abstract description 17
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- 229940126864 fibroblast growth factor Drugs 0.000 title claims abstract description 15
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Developmental Biology & Embryology (AREA)
- Reproductive Health (AREA)
- Gynecology & Obstetrics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US09/522,030 US7005252B1 (en) | 2000-03-09 | 2000-03-09 | Serum free cultivation of primate embryonic stem cells |
| PCT/US2001/006912 WO2001066697A2 (en) | 2000-03-09 | 2001-03-02 | Serum free cultivation of primate embryonic stem cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| NZ520701A true NZ520701A (en) | 2004-03-26 |
Family
ID=24079156
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NZ520701A NZ520701A (en) | 2000-03-09 | 2001-03-02 | Serum free cultivation of primate embryonic stem cells with fibroblast growth factor supplied seperately from fibroblast feeder layers |
Country Status (14)
| Country | Link |
|---|---|
| US (4) | US7005252B1 (https=) |
| EP (1) | EP1261691B1 (https=) |
| JP (3) | JP5717311B2 (https=) |
| KR (1) | KR100795760B1 (https=) |
| CN (1) | CN100372928C (https=) |
| AU (2) | AU4197301A (https=) |
| BR (1) | BR0108507A (https=) |
| CA (1) | CA2402299C (https=) |
| IL (2) | IL151270A0 (https=) |
| IS (1) | IS6515A (https=) |
| MX (1) | MXPA02008698A (https=) |
| NO (1) | NO335780B1 (https=) |
| NZ (1) | NZ520701A (https=) |
| WO (1) | WO2001066697A2 (https=) |
Families Citing this family (152)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7413904B2 (en) * | 1998-10-23 | 2008-08-19 | Geron Corporation | Human embryonic stem cells having genetic modifications |
| US7410798B2 (en) * | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| US10638734B2 (en) | 2004-01-05 | 2020-05-05 | Abt Holding Company | Multipotent adult stem cells, sources thereof, methods of obtaining and maintaining same, methods of differentiation thereof, methods of use thereof and cells derived thereof |
| US7838289B2 (en) | 2001-02-14 | 2010-11-23 | Abt Holding Company | Assay utilizing multipotent adult stem cells |
| US8252280B1 (en) | 1999-08-05 | 2012-08-28 | Regents Of The University Of Minnesota | MAPC generation of muscle |
| US7015037B1 (en) | 1999-08-05 | 2006-03-21 | Regents Of The University Of Minnesota | Multiponent adult stem cells and methods for isolation |
| US7455983B2 (en) * | 2000-01-11 | 2008-11-25 | Geron Corporation | Medium for growing human embryonic stem cells |
| US7439064B2 (en) * | 2000-03-09 | 2008-10-21 | Wicell Research Institute, Inc. | Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium |
| US20100173410A1 (en) * | 2000-03-09 | 2010-07-08 | Wicell Research Institute, Inc. | Cultivation of Primate Embryonic Stem Cells |
| IL159895A0 (en) * | 2001-07-20 | 2004-06-20 | Technion Res & Dev Foundation | Methods of generating human cardiac cells and tissues and uses thereof |
| AU2002364506A1 (en) * | 2001-11-09 | 2003-06-10 | Trustees Of The University Of Pennsylvania | Compositions for the derivation of germ cells from stem cells and methods of use thereof |
| GB0202149D0 (en) | 2002-01-30 | 2002-03-20 | Univ Edinburgh | Pluripotency determining factors and uses thereof |
| US20040111285A1 (en) * | 2002-04-09 | 2004-06-10 | Mark Germain | Method for human pluripotent stem cells |
| WO2004018654A2 (en) * | 2002-08-22 | 2004-03-04 | Celltran Limited | Cell culture surface |
| CN1717478A (zh) * | 2002-10-25 | 2006-01-04 | 湖南惠霖生命科技有限公司 | 用于人类胚胎干细胞体外培养的饲养细胞层以及培养胚胎干细胞的方法 |
| GB0304918D0 (en) * | 2003-03-05 | 2003-04-09 | Celltran Ltd | Cell culture |
| US7820439B2 (en) | 2003-09-03 | 2010-10-26 | Reliance Life Sciences Pvt Ltd. | In vitro generation of GABAergic neurons from pluripotent stem cells |
| US20070269412A1 (en) * | 2003-12-02 | 2007-11-22 | Celavie Biosciences, Llc | Pluripotent cells |
| WO2005056755A2 (en) | 2003-12-02 | 2005-06-23 | Catholic Healthcare West | Compositions and methods for propagation of neural progenitor cells |
| US7541185B2 (en) * | 2003-12-23 | 2009-06-02 | Cythera, Inc. | Methods for identifying factors for differentiating definitive endoderm |
| WO2007059007A2 (en) | 2005-11-14 | 2007-05-24 | Cythera, Inc. | Markers of definitive endoderm |
| US7985585B2 (en) | 2004-07-09 | 2011-07-26 | Viacyte, Inc. | Preprimitive streak and mesendoderm cells |
| US20050266554A1 (en) * | 2004-04-27 | 2005-12-01 | D Amour Kevin A | PDX1 expressing endoderm |
| US8647873B2 (en) | 2004-04-27 | 2014-02-11 | Viacyte, Inc. | PDX1 expressing endoderm |
| CN103898047B (zh) | 2003-12-23 | 2020-03-03 | 维亚希特公司 | 定形内胚层 |
| US7625753B2 (en) * | 2003-12-23 | 2009-12-01 | Cythera, Inc. | Expansion of definitive endoderm cells |
| US20050233446A1 (en) * | 2003-12-31 | 2005-10-20 | Parsons Xuejun H | Defined media for stem cell culture |
| KR20060114381A (ko) * | 2004-02-13 | 2006-11-06 | 레프로셀 인코퍼레이티드 | 배아줄기세포용 피더세포 작성 배지 및 피더세포 |
| CA2560581C (en) * | 2004-03-23 | 2013-06-11 | Toshihiro Akaike | Pluripotent stem cell growing method |
| EP2377922B1 (en) | 2004-04-27 | 2020-04-08 | Viacyte, Inc. | PDX1 expressing endoderm |
| KR20070029745A (ko) | 2004-05-21 | 2007-03-14 | 위셀 리서치 인스티튜트, 인크. | 배아 줄기 세포의 영양 세포 독립적 장기간 배양 |
| NZ582597A (en) | 2004-07-09 | 2011-08-26 | Viacyte Inc | Preprimitive streak and mesendoderm cells |
| EP1791952A4 (en) * | 2004-08-13 | 2008-06-11 | Univ Georgia Res Found | COMPOSITIONS AND METHODS OF SELF-RENEWAL AND DIFFERENTIATION IN HUMAN EMBRYONAL STEM CELLS |
| CN101052712A (zh) * | 2004-09-08 | 2007-10-10 | 威斯康星校友研究基金会 | 培养人胚胎干细胞 |
| ES2800973T3 (es) * | 2004-09-28 | 2021-01-07 | Wisconsin Alumni Res Found | Cultivo de células madre embrionarias de primates |
| US8017395B2 (en) | 2004-12-17 | 2011-09-13 | Lifescan, Inc. | Seeding cells on porous supports |
| EP1844139A1 (en) * | 2004-12-30 | 2007-10-17 | Stemlifeline, Inc. | Methods and systems relating to embryonic stem cell lines |
| US20060275899A1 (en) * | 2004-12-30 | 2006-12-07 | Stemlifeline, Inc. | Methods and compositions relating to embryonic stem cell lines |
| CN1298843C (zh) * | 2005-02-07 | 2007-02-07 | 十堰市太和医院 | 不依赖饲养细胞的人胚干细胞培养基 |
| AU2006202209B2 (en) * | 2005-05-27 | 2011-04-14 | Lifescan, Inc. | Amniotic fluid derived cells |
| CA2613889A1 (en) * | 2005-06-08 | 2006-12-14 | Centocor, Inc. | A cellular therapy for ocular degeneration |
| KR20160116024A (ko) * | 2005-06-22 | 2016-10-06 | 아스테리아스 바이오세라퓨틱스, 인크. | 인간 배아 줄기 세포의 현탁 배양 |
| US9101590B2 (en) * | 2005-07-29 | 2015-08-11 | Yale University | Defined culture conditions of human embryonic stem cells |
| KR100670616B1 (ko) * | 2005-08-25 | 2007-01-17 | 주식회사 메디아나전자 | 상피세포 또는 섬유아세포를 이용한 세포 배양용 인공 배아 및 세포 배양 장치 |
| BRPI0617085A2 (pt) * | 2005-09-02 | 2016-11-08 | Agency Science Tech & Res | método para obter uma célula-tronco mesenquimal (msc), célula ou linhagem celular, método para derivar uma cultura celular a partir de uma célula-tronco embrionária, método para tratamento de uma doença, uso de uma célula-tronco mesenquimal, linhagem diferenciada, composição farmacêutica, método para condicionar um meio de cultura celular, meio condicionado e uso do mesmo, mpetodo para obterum polipeptídeo, método para obtar uma cultura celular, cultura celular, célula-tronco mesenquimal, linhagem de célula-tronco mesenquimal ou uma célula mesenquimal diferenciada |
| US7682799B2 (en) * | 2005-10-06 | 2010-03-23 | University Of Massachusetts | Cell division marker |
| EP1945758A2 (en) * | 2005-10-07 | 2008-07-23 | Cellartis AB | A method for obtaining a xeno-free hbs cell line |
| AU2006305879B2 (en) | 2005-10-27 | 2012-05-10 | Viacyte, Inc. | PDX1-expressing dorsal and ventral foregut endoderm |
| US7413900B2 (en) * | 2005-10-31 | 2008-08-19 | President And Fellows Of Harvard College | Immortalized fibroblasts |
| EP1999253B1 (en) * | 2006-03-02 | 2019-05-22 | Viacyte, Inc. | Endocrine precursor cells, pancreatic hormone-expressing cells and methods of production |
| US11254916B2 (en) | 2006-03-02 | 2022-02-22 | Viacyte, Inc. | Methods of making and using PDX1-positive pancreatic endoderm cells |
| US7695965B2 (en) | 2006-03-02 | 2010-04-13 | Cythera, Inc. | Methods of producing pancreatic hormones |
| CA2650812C (en) | 2006-04-28 | 2017-12-12 | Lifescan, Inc. | Differentiation of human embryonic stem cells |
| US8741643B2 (en) | 2006-04-28 | 2014-06-03 | Lifescan, Inc. | Differentiation of pluripotent stem cells to definitive endoderm lineage |
| US7989204B2 (en) * | 2006-04-28 | 2011-08-02 | Viacyte, Inc. | Hepatocyte lineage cells |
| CN100465268C (zh) * | 2006-05-17 | 2009-03-04 | 北京大学 | 人胚胎干细胞的培养方法及其专用培养基 |
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