NO960484L - Anvendelsen av exonuklease og/eller glykosylase som supplement til anti-polymerase-antistoff for å öke spesifisiteten i polymerasekjedereaksjon - Google Patents

Anvendelsen av exonuklease og/eller glykosylase som supplement til anti-polymerase-antistoff for å öke spesifisiteten i polymerasekjedereaksjon

Info

Publication number
NO960484L
NO960484L NO960484A NO960484A NO960484L NO 960484 L NO960484 L NO 960484L NO 960484 A NO960484 A NO 960484A NO 960484 A NO960484 A NO 960484A NO 960484 L NO960484 L NO 960484L
Authority
NO
Norway
Prior art keywords
polymerase
glycosylase
exonuclease
adjunct
chain reaction
Prior art date
Application number
NO960484A
Other languages
English (en)
Other versions
NO960484D0 (no
Inventor
John William Hender Sutherland
David Robert Patterson
Original Assignee
Johnson & Johnson Clin Diag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johnson & Johnson Clin Diag filed Critical Johnson & Johnson Clin Diag
Publication of NO960484D0 publication Critical patent/NO960484D0/no
Publication of NO960484L publication Critical patent/NO960484L/no

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6804—Nucleic acid analysis using immunogens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/319—Exonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/50—Other enzymatic activities
    • C12Q2521/531—Glycosylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Pathology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Saccharide Compounds (AREA)

Abstract

Blandinger og metoder for PCR-forsterknlng av mål- nuklelneyrer der forsterknlngseffektivlteten økes ved å Innarbeide et antistoff som er spesifikt for et polymerlserlngsmlddel og minst en av en exonuklease og en glykosylase l PCR-reaksJonsblandlngen. Videre beskrives sett for forsterkning av målnukleln- syren.
NO960484A 1995-02-07 1996-02-06 Anvendelsen av exonuklease og/eller glykosylase som supplement til anti-polymerase-antistoff for å öke spesifisiteten i polymerasekjedereaksjon NO960484L (no)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US38501995A 1995-02-07 1995-02-07

Publications (2)

Publication Number Publication Date
NO960484D0 NO960484D0 (no) 1996-02-06
NO960484L true NO960484L (no) 1996-08-08

Family

ID=23519709

Family Applications (1)

Application Number Title Priority Date Filing Date
NO960484A NO960484L (no) 1995-02-07 1996-02-06 Anvendelsen av exonuklease og/eller glykosylase som supplement til anti-polymerase-antistoff for å öke spesifisiteten i polymerasekjedereaksjon

Country Status (15)

Country Link
US (1) US5985619A (no)
EP (1) EP0726324B1 (no)
JP (1) JPH08266298A (no)
KR (1) KR960031627A (no)
AT (1) ATE201452T1 (no)
AU (1) AU709788B2 (no)
CA (1) CA2168712A1 (no)
DE (1) DE69612885T2 (no)
DK (1) DK0726324T3 (no)
ES (1) ES2156978T3 (no)
FI (1) FI960540A (no)
GR (1) GR3036391T3 (no)
NO (1) NO960484L (no)
PT (1) PT726324E (no)
SI (1) SI0726324T1 (no)

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US6994960B1 (en) * 1997-05-28 2006-02-07 The Walter And Eliza Hall Institute Of Medical Research Nucleic acid diagnostics based on mass spectrometry or mass separation and base specific cleavage
US6300075B1 (en) * 1999-02-03 2001-10-09 Ortho-Clinical Diagnostics, Inc Enhancement of the specificity of nucleic acid amplification by carrier nucleic acid
US7074556B2 (en) * 1999-03-02 2006-07-11 Invitrogen Corporation cDNA synthesis improvements
FR2803601A1 (fr) * 2000-01-11 2001-07-13 Inst Nat Sante Rech Med Utilisation de l'exonuclease iii dans des reactions d'amplification par polymerase de fragments d'adn
EP1275735A1 (en) 2001-07-11 2003-01-15 Roche Diagnostics GmbH Composition and method for hot start nucleic acid amplification
US20040152072A1 (en) * 2002-07-30 2004-08-05 Invitrogen Corporation Reverse transcription
PT1526187E (pt) 2003-05-01 2008-10-16 Veridex Llc Ensaios de gânglios linfáticos intra-operatórios
MXPA06014175A (es) * 2004-06-04 2007-05-09 Johnson & Johnson Diagnostico o pronostico del curso del cancer de mama.
US20070154889A1 (en) * 2004-06-25 2007-07-05 Veridex, Llc Methods and reagents for the detection of melanoma
ES2364983T3 (es) * 2006-02-27 2011-09-20 F. Hoffmann-La Roche Ag Reacción en cadena de polimerasa con arranque en caliente por secuestro de magnesio.
EP2019687B1 (en) 2006-03-29 2014-03-19 Merial Limited Vaccine against streptococci
CA2658520C (en) * 2008-03-19 2016-11-08 F. Hoffmann-La Roche Ag Nucleic acid amplification in the presence of modified randomers
EP2990487A1 (en) 2008-05-08 2016-03-02 Asuragen, INC. Compositions and methods related to mirna modulation of neovascularization or angiogenesis
US7910720B2 (en) 2008-09-09 2011-03-22 Roche Diagnostics Operations, Inc. Polyanion for improved nucleic acid amplification
US8597892B2 (en) 2009-05-22 2013-12-03 Asuragen, Inc. MiRNA biomarkers of prostate disease
IL206810A (en) 2009-07-08 2015-07-30 Janssen Diagnostics Llc Methods for identifying melanoma and kits for using these methods
CN102725632A (zh) 2009-08-28 2012-10-10 奥斯瑞根公司 肺病的miRNA生物标志物
WO2012068400A2 (en) 2010-11-17 2012-05-24 Asuragen, Inc. Mirnas as biomarkers for distinguishing benign from malignant thyroid neoplasms
WO2014145612A1 (en) 2013-03-15 2014-09-18 Ajay Goel Tissue and blood-based mirna biomarkers for the diagnosis, prognosis and metastasis-predictive potential in colorectal cancer
CA2905949A1 (en) 2013-03-15 2014-09-25 Baylor Research Institute Ulcerative colitis (uc)-associated colorectal neoplasia markers
US20150191794A1 (en) 2014-01-05 2015-07-09 Biomirna Holdings Ltd. Lung Cancer Determinations Using MIRNA
US10619210B2 (en) 2014-02-07 2020-04-14 The Johns Hopkins University Predicting response to epigenetic drug therapy
GB201414745D0 (en) 2014-08-19 2014-10-01 Articzymes As Exonucleases
WO2016094330A2 (en) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Methods and machine learning systems for predicting the liklihood or risk of having cancer
EP3292214A1 (en) 2015-05-04 2018-03-14 Academisch Medisch Centrum Method of quantifying mirnas using normalization
US10081829B1 (en) 2017-06-13 2018-09-25 Genetics Research, Llc Detection of targeted sequence regions
JP2019198259A (ja) * 2018-05-15 2019-11-21 株式会社島津製作所 １ステップｒｔ−ｐｃｒ法
WO2020172362A1 (en) * 2019-02-20 2020-08-27 Genetics Research, Llc Regulated multiplex reactions in a single tube
EP3719144A1 (en) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p as an universal endogenous control for exosome cargo normalization
EP3999664A1 (en) 2019-07-19 2022-05-25 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Method for determining the response to treatment of a patient affected by non-small cell lung carcinoma (nsclc)
CN114787193B (zh) * 2019-12-19 2023-04-21 深圳华大生命科学研究院 抗聚合酶的单克隆抗体及其应用
WO2023146243A1 (ko) * 2022-01-26 2023-08-03 주식회사 씨젠 샘플 내 타겟 핵산을 검출하는 방법
CN116773291B (zh) * 2023-03-31 2024-04-19 艾普拜生物科技(苏州)有限公司 一种石蜡切片dna预处理试剂及方法

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EP0487218B1 (en) * 1990-10-31 1997-12-29 Tosoh Corporation Method for detecting or quantifying target nucleic acid
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US5338671A (en) * 1992-10-07 1994-08-16 Eastman Kodak Company DNA amplification with thermostable DNA polymerase and polymerase inhibiting antibody
US5605796A (en) * 1994-07-19 1997-02-25 Behringwerke Ag Method for preventing amplification of nucleic acid contaminants in amplification mixtures using nuclease-receptor conjugates

Also Published As

Publication number Publication date
AU709788B2 (en) 1999-09-09
AU4339196A (en) 1996-08-15
JPH08266298A (ja) 1996-10-15
US5985619A (en) 1999-11-16
EP0726324A2 (en) 1996-08-14
CA2168712A1 (en) 1996-08-08
KR960031627A (ko) 1996-09-17
ES2156978T3 (es) 2001-08-01
EP0726324A3 (en) 1996-11-27
GR3036391T3 (en) 2001-11-30
DK0726324T3 (da) 2001-07-30
ATE201452T1 (de) 2001-06-15
PT726324E (pt) 2001-08-30
FI960540A0 (fi) 1996-02-06
FI960540A (fi) 1996-08-08
SI0726324T1 (en) 2001-12-31
NO960484D0 (no) 1996-02-06
DE69612885D1 (de) 2001-06-28
EP0726324B1 (en) 2001-05-23
DE69612885T2 (de) 2001-09-13

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