NO330906B1 - In vitro fremgangsmate for pavisning av hoyrisiko HPV indusert celletransformasjon og anvendelsen av denne - Google Patents
In vitro fremgangsmate for pavisning av hoyrisiko HPV indusert celletransformasjon og anvendelsen av denne Download PDFInfo
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- NO330906B1 NO330906B1 NO20002979A NO20002979A NO330906B1 NO 330906 B1 NO330906 B1 NO 330906B1 NO 20002979 A NO20002979 A NO 20002979A NO 20002979 A NO20002979 A NO 20002979A NO 330906 B1 NO330906 B1 NO 330906B1
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PCT/US1998/026447 WO1999029890A2 (fr) | 1997-12-12 | 1998-12-11 | Evaluation de maladies liees au virus du papillome humain |
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EP (2) | EP1038022B1 (fr) |
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CA (2) | CA2313483C (fr) |
DE (1) | DE69830927T2 (fr) |
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NO (2) | NO330906B1 (fr) |
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Families Citing this family (78)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
BR9814271A (pt) * | 1997-12-12 | 2001-03-20 | Digene Corp | Determinação de doenças relacionadas com o vìrus do papiloma humano |
WO2001027857A2 (fr) | 1999-10-13 | 2001-04-19 | Sequenom, Inc. | Methodes de generation de bases de donnees et bases de donnees pour l'identification de marqueurs genetiques polymorphes |
KR100382703B1 (ko) * | 2000-03-15 | 2003-05-09 | 주식회사 바이오메드랩 | 인유두종바이러스의 유전형 진단키트 및 상기 진단키트의제조방법 |
AU2001249526A1 (en) * | 2000-03-28 | 2001-10-08 | Biosearch International Pty Ltd | Approaches for hpv detection and staging by targeting the e6 gene region of the viral genome |
US20040115692A1 (en) * | 2000-04-03 | 2004-06-17 | Cytyc Corporation | Methods, compositions and apparatuses for detecting a target in a preservative solution |
WO2001075174A2 (fr) * | 2000-04-03 | 2001-10-11 | Cytyc Corporation | Detection et typage du papillomavirus humain a l'aide de sondes de proteines pna |
IT1316070B1 (it) * | 2000-05-05 | 2003-03-28 | Bioanalisi Ct Sud Snc Di Perse | Metodo e mezzi per l'identificazione di virus hpv |
US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
US7439016B1 (en) * | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
EP1294853B1 (fr) | 2000-06-21 | 2010-05-05 | QIAGEN Gaithersburg, Inc. | Milieu de prelevement universel |
GB0018140D0 (en) * | 2000-07-24 | 2000-09-13 | Medical Res Council | Screening for abnormalities |
US20040229298A1 (en) * | 2000-11-11 | 2004-11-18 | Lu Peter S. | Methods and compositions for treating cervical cancer |
US20040121310A1 (en) * | 2002-12-18 | 2004-06-24 | Ecker David J. | Methods for rapid detection and identification of bioagents in forensic studies |
KR100437626B1 (ko) * | 2001-06-21 | 2004-06-26 | 주식회사 마이진 | 인간 유두종바이러스 유전자형 검사 방법과 이를 위한검사 키트 |
US7553623B2 (en) * | 2002-01-07 | 2009-06-30 | Norchip A/S | Method for detecting human papillomavirus mRNA |
ATE465753T1 (de) | 2002-10-21 | 2010-05-15 | Eisai Inc | Zusammensetzungen und verfahren zurbehandlung von krankheiten, die durch das humane papillomavirus vermitteltwerden |
ES2298467T3 (es) * | 2003-03-31 | 2008-05-16 | Stichting Researchfonds Pathologie | Deteccion de canceres invasivos inducidos por hpv y sus lesiones precursoras con potencial invasivo. |
US20040203004A1 (en) * | 2003-04-10 | 2004-10-14 | Bernard Hans Ulrich | Diagnostic apparatus and method |
US7361460B2 (en) | 2003-04-11 | 2008-04-22 | Digene Corporation | Approach to molecular diagnosis of human papillomavirus-related diseases |
US20070065810A1 (en) * | 2003-07-18 | 2007-03-22 | Georgetown University | Diagnosis and treatment of cervical cancer |
EP1510820B1 (fr) * | 2003-08-25 | 2010-03-17 | MTM Laboratories AG | Procédé pour la détection d'états médicalement pertinents dans des échantillons LBC solubilisés |
ATE289685T1 (de) | 2003-08-25 | 2005-03-15 | Mtm Lab Ag | Verfahren zum nachweis von karzinomen in solubilisierten zervikalen körperproben |
US7482142B1 (en) | 2004-05-07 | 2009-01-27 | Roche Molecular Systems, Inc. | High-risk human papillomavirus detection |
US7316904B1 (en) | 2004-06-30 | 2008-01-08 | Chromodynamics, Inc. | Automated pap screening using optical detection of HPV with or without multispectral imaging |
KR100572721B1 (ko) * | 2004-07-26 | 2006-04-24 | 이미영 | 환원전리수를 포함하는 rna 보존액 및 그를 이용한rna 보존방법 |
ATE476528T1 (de) | 2004-12-08 | 2010-08-15 | Gen Probe Inc | Nukleinsäuredetektion aus verschiedenen typen des humanen papillomavirus |
US20060161076A1 (en) * | 2005-01-06 | 2006-07-20 | Diamics, Inc. | Systems and methods for collection of cell clusters |
US20060189893A1 (en) * | 2005-01-06 | 2006-08-24 | Diamics, Inc. | Systems and methods for detecting abnormal cells |
GB0500996D0 (en) * | 2005-01-18 | 2005-02-23 | Delfts Diagnostic Labaratory B | Detection method and materials therefor |
US7524631B2 (en) * | 2005-02-02 | 2009-04-28 | Patterson Bruce K | HPV E6, E7 mRNA assay and methods of use thereof |
US20070111960A1 (en) * | 2005-03-04 | 2007-05-17 | Advandx, Inc. | High affinity probes for analysis of human papillomavirus expression |
DE102005015005A1 (de) * | 2005-04-01 | 2006-10-05 | Qiagen Gmbh | Verfahren zur Behandlung einer Biomoleküle enthaltenden Probe |
US7732166B2 (en) * | 2005-11-15 | 2010-06-08 | Oncohealth Corporation | Detection method for human pappilomavirus (HPV) and its application in cervical cancer |
US7972776B2 (en) * | 2005-11-15 | 2011-07-05 | Oncohealth Corporation | Protein chips for HPV detection |
JP5377298B2 (ja) * | 2006-06-19 | 2013-12-25 | ベクトン・ディキンソン・アンド・カンパニー | 輸送培地に曝露した組織、細胞、またはウイルスから増幅可能な核酸を取得するための方法および組成物 |
EP2484781B1 (fr) * | 2006-08-01 | 2017-08-23 | Applied Biosystems, LLC | Détection d'analytes et d'acides nucléiques |
US8916342B2 (en) | 2006-11-13 | 2014-12-23 | Oncohealth Corp. | Identification of high grade or ≧ CIN2 for early stages and late stages detection, screening, and diagnosis of human papillomavirus (HPV) and HPV-associated cancers |
US8278056B2 (en) | 2008-06-13 | 2012-10-02 | Oncohealth Corp. | Detection of early stages and late stages HPV infection |
US8968995B2 (en) * | 2008-11-12 | 2015-03-03 | Oncohealth Corp. | Detection, screening, and diagnosis of HPV-associated cancers |
WO2008071998A2 (fr) * | 2006-12-15 | 2008-06-19 | Oncomethylome Sciences Sa | Procédés améliorés de diagnostic de maladies impliquant une infection à pvh |
JP5241100B2 (ja) * | 2006-12-28 | 2013-07-17 | シスメックス株式会社 | 抗原賦活化液、抗原賦活化方法及び細胞の検出方法 |
US20090311392A1 (en) * | 2007-09-20 | 2009-12-17 | Paul Bernard Newman | Novel approach to the controlled decontamination and or detoxification of nuts, grains, fruits and vegetables |
WO2009092017A1 (fr) * | 2008-01-16 | 2009-07-23 | Medical College Of Georgia Research Institute, Inc. | Marqueurs biologiques pour le cancer induit par le hpv |
AU2009238247B2 (en) * | 2008-04-17 | 2014-12-11 | Qiagen Gaithersburg, Inc. | Compositions, methods, and kits using synthetic probes for determining the presence of a target nucleic acid |
US9782565B2 (en) | 2008-10-01 | 2017-10-10 | Covidien Lp | Endoscopic ultrasound-guided biliary access system |
US11298113B2 (en) | 2008-10-01 | 2022-04-12 | Covidien Lp | Device for needle biopsy with integrated needle protection |
US8968210B2 (en) | 2008-10-01 | 2015-03-03 | Covidien LLP | Device for needle biopsy with integrated needle protection |
US9186128B2 (en) | 2008-10-01 | 2015-11-17 | Covidien Lp | Needle biopsy device |
US9332973B2 (en) | 2008-10-01 | 2016-05-10 | Covidien Lp | Needle biopsy device with exchangeable needle and integrated needle protection |
CA2741650C (fr) * | 2008-10-27 | 2017-09-05 | Qiagen Gaithersburg Inc. | Essai et systeme de capture d'hybride a resultats rapides |
EP2184368A1 (fr) | 2008-11-07 | 2010-05-12 | DKFZ Deutsches Krebsforschungszentrum | Transcription de diagnostic et motifs d'épissage du HPV16 dans différentes lésions cervicales |
GB0820822D0 (en) * | 2008-11-13 | 2008-12-24 | Inst Catala D Oncologia | Novel product and processes |
WO2010064628A1 (fr) * | 2008-12-05 | 2010-06-10 | オリンパス株式会社 | Procédé pour préparer un échantillon contenant un acide nucléique, solution pour préparer un échantillon, et procédé pour analyser un acide nucléique |
WO2010085841A1 (fr) | 2009-01-27 | 2010-08-05 | Genetic Technologies Limited | Dispositif de prélèvement d'échantillon biologique |
CA2750338C (fr) * | 2009-01-28 | 2019-06-25 | Qiagen Gaithersburg, Inc. | Procede de preparation d'un echantillon de grand volume specifique a une sequence et dosage |
WO2010127228A1 (fr) | 2009-05-01 | 2010-11-04 | Qiagen Gaithersburg, Inc. | Procédé d'amplification non ciblée pour la détection de formes d'épissage d'arn dans un échantillon |
US10190152B2 (en) | 2009-09-03 | 2019-01-29 | Becton, Dickinson And Company | Methods and compositions for direct chemical lysis |
CA2773320C (fr) | 2009-09-14 | 2018-02-20 | Qiagen Gaithersburg, Inc. | Compositions et methodes pour la recuperation d'acides nucleiques ou de proteines a partir d'echantillons de tissus fixes dans des milieux de cytologie |
EP2521778B1 (fr) * | 2010-01-04 | 2014-03-19 | QIAGEN Gaithersburg, Inc. | Procédés, compositions et kits de récupération des acides nucléiques ou des protéines dans des échantillons de tissus fixés |
EP2521914A4 (fr) | 2010-01-08 | 2013-07-10 | Oncohealth Corp | Immunoessais sur le vph à base de cellule et à haut débit pour diagnostiquer et dépister des cancers associés au vph |
CA2787781A1 (fr) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Procede de determination de confirmation de la presence d'un hpv dans un echantillon |
ES2615728T3 (es) * | 2010-01-29 | 2017-06-08 | Qiagen Gaithersburg, Inc. | Métodos y composiciones para una purificación y un análisis múltiple específico de secuencia de ácidos nucleicos |
US9422593B2 (en) | 2010-05-19 | 2016-08-23 | Qiagen Gaithresburg, Inc | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
AU2011258501B2 (en) | 2010-05-25 | 2016-07-07 | Qiagen Gaithersburg, Inc. | Fast results hybrid capture assay and associated strategically-truncated probes |
JP2014511182A (ja) * | 2011-02-24 | 2014-05-15 | キアゲン ガイサーズバーグ アイエヌシー. | Hpv核酸を検出するための材料及び方法 |
WO2012176065A2 (fr) * | 2011-06-24 | 2012-12-27 | Biotechnology Developers, S.A. | Méthode, compositions et dispositif de préparation de prélèvements cytologiques |
CA2854165C (fr) | 2011-11-03 | 2022-11-22 | Qiagen Gaithersburg, Inc. | Substances et procede pour immobiliser, isoler et concentrer des cellules en utilisant des surfaces carboxylees |
JP5960421B2 (ja) * | 2011-12-05 | 2016-08-02 | 株式会社日立ハイテクノロジーズ | 細胞の測定方法及び細胞の測定用試薬 |
GB2515713B (en) | 2012-04-28 | 2019-06-05 | Medite Cancer Diagnostics Inc | Methods, packaging and apparatus for collection of biological samples |
US8734364B1 (en) | 2013-11-07 | 2014-05-27 | Genetic Technologies Limited | Device and method for obtaining a biological sample |
WO2016005789A1 (fr) | 2014-07-07 | 2016-01-14 | Institut Pasteur | Transcriptome de papillomavirus à large spectre génétique et génotypique en tant que biomarqueur de stades du cancer associé à papillomavirus |
EP3225685B1 (fr) | 2015-06-30 | 2018-12-12 | Sysmex Corporation | Solution de conservation de cellules, son utilisation et procédé de production d'une solution de conservation de cellules |
CA3037086A1 (fr) | 2016-10-03 | 2018-04-12 | Juno Therapeutics, Inc. | Molecules se liant specifiquement au vph |
CA3080546A1 (fr) | 2017-10-03 | 2019-04-11 | Juno Therapeutics, Inc. | Molecules de liaison specifique a l'hpv |
TWI840351B (zh) | 2018-04-05 | 2024-05-01 | 美商奇諾治療有限公司 | T細胞受體及表現其之工程化細胞 |
PL240016B1 (pl) * | 2019-09-09 | 2022-02-07 | Genomtec Spolka Akcyjna | Zestaw starterów do wykrywania wirusa brodawczaka ludzkiego typ 16 (HPV16 Human papillomavirus type 16), sposób wykrywania infekcji HPV16, zastosowanie zestawu starterów do wykrywania infekcji HPV16 |
EP4100051A2 (fr) * | 2020-02-07 | 2022-12-14 | ISA Pharmaceuticals B.V. | Traitement de maladies liées au hpv |
WO2023081900A1 (fr) | 2021-11-08 | 2023-05-11 | Juno Therapeutics, Inc. | Lymphocytes t modifiés exprimant un récepteur recombiné de lymphocytes t (tcr) et systèmes et procédés apparentés |
Family Cites Families (100)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4732847A (en) * | 1981-06-09 | 1988-03-22 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
FI63596C (fi) * | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
US4578282A (en) * | 1982-02-04 | 1986-03-25 | Harrison James S | Composition for diagnostic reagents |
US4465078A (en) | 1982-09-30 | 1984-08-14 | Medtest Corporation | Method for cell sampling in a body cavity |
US4865980A (en) * | 1982-12-29 | 1989-09-12 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
US5288611A (en) * | 1983-01-10 | 1994-02-22 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating organisms and viruses |
EP0756010B1 (fr) * | 1983-01-10 | 2001-07-04 | Gen-Probe Incorporated | Procédé de détection, identification et quantification d'organismes et de virus |
US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
EP0144914A3 (fr) | 1983-12-12 | 1986-08-13 | Miles Inc. | Test d'hybridation utilisant des paires marquées de réactifs pouvant lier un hybride |
US4743535A (en) * | 1984-11-07 | 1988-05-10 | Miles Inc. | Hybridization assay employing labeled probe and anti-hybrid |
AU3844485A (en) * | 1984-02-09 | 1985-08-15 | Enzo Biochem Inc. | Heterologous detection of cabeled dna |
FI71768C (fi) * | 1984-02-17 | 1987-02-09 | Orion Yhtymae Oy | Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning. |
US6221581B1 (en) * | 1984-04-27 | 2001-04-24 | Enzo Diagnostics, Inc. | Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions |
CA1260372A (fr) * | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Methode d'hybridation pour la detection de materiaux genetiques |
ZA853756B (en) | 1984-06-01 | 1986-01-29 | Miles Lab | Nucleic acid hybridization assay employing immobilized rna probes |
CA1253777A (fr) | 1984-06-01 | 1989-05-09 | Robert J. Carrico | Essai d'hybridation de l'acide nucleique au moyen de sondes arn immobilisees |
US4563417A (en) * | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
US4775619A (en) * | 1984-10-16 | 1988-10-04 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
US5641630A (en) * | 1985-06-13 | 1997-06-24 | Amgen Inc. | Method and kit for performing nucleic acid hybridization assays |
US4888278A (en) * | 1985-10-22 | 1989-12-19 | University Of Massachusetts Medical Center | In-situ hybridization to detect nucleic acid sequences in morphologically intact cells |
US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
US5750338A (en) * | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
IL85551A0 (en) | 1987-04-01 | 1988-08-31 | Miles Inc | Rapid hybridization assay and reagent system used therein |
GB8717169D0 (en) * | 1987-07-21 | 1987-08-26 | Ti Interlock Ltd | Pneumatic control system |
US5656731A (en) * | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
US5359100A (en) * | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
US5082830A (en) * | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
US6326136B1 (en) * | 1988-04-01 | 2001-12-04 | Digene Corporation | Macromolecular conjugate made using unsaturated aldehydes |
US5374524A (en) * | 1988-05-10 | 1994-12-20 | E. I. Du Pont De Nemours And Company | Solution sandwich hybridization, capture and detection of amplified nucleic acids |
US5639871A (en) * | 1988-09-09 | 1997-06-17 | Roche Molecular Systems, Inc. | Detection of human papillomavirus by the polymerase chain reaction |
AU5269590A (en) * | 1989-03-10 | 1990-10-09 | Gene-Trak Systems | Immobilized oligonucleotide probes and uses therefor |
US5106727A (en) * | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
EP0405913B1 (fr) * | 1989-06-30 | 1998-12-23 | Chiron Corporation | Sonde d'acide nucléique hydrophobe |
US5116734A (en) * | 1989-09-01 | 1992-05-26 | Digene Diagnostics, Inc. | Highly sensitive method for detecting peroxidase |
DE69033850T2 (de) | 1989-12-01 | 2002-06-06 | Vysis, Inc. | Nukleinsäuresonden zum Nachweis von HPV-Transkripts |
US5580970A (en) * | 1989-12-01 | 1996-12-03 | Amoco Corporation | Detection of HPV transcripts |
US5629153A (en) * | 1990-01-10 | 1997-05-13 | Chiron Corporation | Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays |
US5792606A (en) * | 1990-02-26 | 1998-08-11 | Boehringer Mannheim Gmbh | Nucleic acid hybridization based assay for determining a substance of interest |
CA2039517C (fr) * | 1990-04-03 | 2006-11-07 | David Segev | Amplification du signal d'une sonde d'adn |
US5695926A (en) * | 1990-06-11 | 1997-12-09 | Bio Merieux | Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support |
US5484699A (en) * | 1990-09-28 | 1996-01-16 | Abbott Laboratories | Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods |
US5474895A (en) * | 1990-11-14 | 1995-12-12 | Siska Diagnostics Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
US5256571A (en) | 1991-05-01 | 1993-10-26 | Cytyc Corporation | Cell preservative solution |
US5543294A (en) | 1991-07-19 | 1996-08-06 | The Trustees Of Columbia University In The City Of New York | Polymerase chain reaction/restriction fragment length polymorphism method for the detection and typing of myobacteria |
US5556748A (en) * | 1991-07-30 | 1996-09-17 | Xenopore Corporation | Methods of sandwich hybridization for the quantitative analysis of oligonucleotides |
US5981179A (en) * | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
DK0667918T3 (da) * | 1991-11-14 | 2000-06-05 | Dgi Inc | Ikke-radioaktivt hybridiseringsassay og -sæt |
EP0618924B1 (fr) * | 1991-12-23 | 2001-02-14 | Bayer Corporation | Sondes d'amplification de vhb utlisees dans des methodes d'hybridation en sandwich a phase en solution |
US5747244A (en) * | 1991-12-23 | 1998-05-05 | Chiron Corporation | Nucleic acid probes immobilized on polystyrene surfaces |
US5424413A (en) * | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
IL106273A0 (en) | 1992-07-17 | 1993-11-15 | Res Dev Foundation | Rapid detection of biopolymers in stained specimens |
JPH08503606A (ja) * | 1992-10-09 | 1996-04-23 | アモコ・コーポレーション | 検定法 |
AU6031094A (en) * | 1993-01-15 | 1994-08-15 | Public Health Research Institute Of The City Of New York, Inc., The | Sensitive nucleic acid sandwich hybridization assays and kits |
US5357977A (en) * | 1993-04-23 | 1994-10-25 | St. Mary's Hospital And Medical Center, Inc. | Cytological sampling method and device |
WO1994028156A1 (fr) * | 1993-05-20 | 1994-12-08 | Dana-Farber Cancer Institute | Compositions et procedes de traitement d'infections a virus herpetique |
JP3026843B2 (ja) * | 1993-07-23 | 2000-03-27 | ジェン−プローブ・インコーポレーテッド | 核酸増幅の促進法 |
DE4331012A1 (de) * | 1993-09-13 | 1995-03-16 | Bayer Ag | Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik |
US5370128A (en) | 1993-12-02 | 1994-12-06 | Wainwright; Sharon R. | Pap brush and pap unit container systems |
US5681697A (en) * | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
BR9507343A (pt) * | 1994-04-04 | 1997-09-16 | Ciba Corning Diagnostics Corp | Testes de hibridização - ligação para a detecção de sequências de ácido nucléico específicas |
CA2126952C (fr) * | 1994-06-28 | 2010-12-14 | Sithian Pandian | Sonde, kit et methode d'amplification pour augmenter la sensibilite des essais d'hybridation de l'acide nucleique en molecules cibles |
US5681702A (en) * | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
JP3093116B2 (ja) * | 1994-09-30 | 2000-10-03 | 株式会社豊田中央研究所 | 核酸検出方法 |
US6057099A (en) * | 1994-12-02 | 2000-05-02 | Intelligene Ltd. | Detection of nucleic acid sequences |
US5747248A (en) * | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
DE4445769C1 (de) * | 1994-12-21 | 1995-11-09 | Biochrom Kg | Protektionsmedium zum Aufbewahren vitaler Gewebe, insbesondere von Zähnen |
CA2139070C (fr) * | 1994-12-23 | 2010-03-30 | Burton W. Blais | Methode pour ameliorer l'aptitude a la detection d'essais d'acide nucleique utilisant une reaction en chaine de polymerase |
US5731153A (en) * | 1996-08-26 | 1998-03-24 | The Regents Of The University Of California | Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions |
US5888724A (en) * | 1995-02-17 | 1999-03-30 | The Trustees Of Columbia University In The City Of New York | Detection of high oncogenic-risk papilloma virus in high grade cervical lesions and cancers by a PCR/ELISA assay |
US6083925A (en) * | 1995-06-07 | 2000-07-04 | Connaught Laboratories Limited | Nucleic acid respiratory syncytial virus vaccines |
JP2000500342A (ja) * | 1995-11-15 | 2000-01-18 | ジェン−プローブ・インコーポレーテッド | ヒトパピローマウイルス核酸に相補的な核酸プローブおよび関連する方法およびキット |
US5853993A (en) * | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
US5679333A (en) * | 1996-10-25 | 1997-10-21 | Dunphy; Brian William | Formaldehyde-free tissue preservative compositions |
US6110676A (en) * | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
US20020034737A1 (en) * | 1997-03-04 | 2002-03-21 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
US6043038A (en) * | 1998-03-31 | 2000-03-28 | Tularik, Inc. | High-throughput screening assays for modulators of primase activity |
JP2002505071A (ja) * | 1997-11-04 | 2002-02-19 | ロシュ ダイアグノスティックス ゲーエムベーハー | 特異的かつ高感度な核酸検出方法 |
BR9814271A (pt) * | 1997-12-12 | 2001-03-20 | Digene Corp | Determinação de doenças relacionadas com o vìrus do papiloma humano |
US20030096232A1 (en) * | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
US5994079A (en) * | 1998-02-06 | 1999-11-30 | Digene Corporation | Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function |
US7399589B2 (en) | 1998-02-06 | 2008-07-15 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
US6686151B1 (en) * | 1998-02-06 | 2004-02-03 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
US20010055766A1 (en) * | 1999-04-02 | 2001-12-27 | Alexander Aristarkhov | Immunosorbant assay using branched bis-biotin/avidin/multiple label complex as a detection reagent |
US7019822B1 (en) * | 1999-04-29 | 2006-03-28 | Mss, Inc. | Multi-grade object sorting system and method |
US6544732B1 (en) * | 1999-05-20 | 2003-04-08 | Illumina, Inc. | Encoding and decoding of array sensors utilizing nanocrystals |
US6893819B1 (en) * | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
US6436662B1 (en) * | 2000-04-04 | 2002-08-20 | Digene Corporation | Device and method for cytology slide preparation |
US6521190B1 (en) * | 2000-05-19 | 2003-02-18 | Digene Corporation | Cell collection apparatus |
EP1290225A4 (fr) * | 2000-05-20 | 2004-09-15 | Univ Michigan | Procede de production d'une bibliotheque d'adn utilisant l'amplification positionnelle |
US7439016B1 (en) * | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
US7601497B2 (en) * | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
EP1294853B1 (fr) * | 2000-06-21 | 2010-05-05 | QIAGEN Gaithersburg, Inc. | Milieu de prelevement universel |
US6977148B2 (en) * | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
CN101151379A (zh) * | 2005-01-14 | 2008-03-26 | 密执安州立大学董事会 | 检测生物样品中的人乳头瘤病毒的系统、方法和组合物 |
US7972776B2 (en) * | 2005-11-15 | 2011-07-05 | Oncohealth Corporation | Protein chips for HPV detection |
EP2413142B1 (fr) * | 2007-02-27 | 2013-06-05 | Nuclea Biomarkers LLC | Méthode de prédiction de la réponse à un traitment par un inhibiteur de tyrosine kinase du récepteur à l'EGF des patients atteints de carcinome non à petites cellules |
US7985375B2 (en) * | 2007-04-06 | 2011-07-26 | Qiagen Gaithersburg, Inc. | Sample preparation system and method for processing clinical specimens |
AU2009238247B2 (en) * | 2008-04-17 | 2014-12-11 | Qiagen Gaithersburg, Inc. | Compositions, methods, and kits using synthetic probes for determining the presence of a target nucleic acid |
US9090948B2 (en) * | 2008-09-30 | 2015-07-28 | Abbott Molecular Inc. | Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples |
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- 1998-12-11 EP EP98963881A patent/EP1038022B1/fr not_active Revoked
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