NO319296B1 - Fremgangsmate og reagens-kit for pavisning av en analytt i en provevaeske. - Google Patents
Fremgangsmate og reagens-kit for pavisning av en analytt i en provevaeske. Download PDFInfo
- Publication number
- NO319296B1 NO319296B1 NO19975937A NO975937A NO319296B1 NO 319296 B1 NO319296 B1 NO 319296B1 NO 19975937 A NO19975937 A NO 19975937A NO 975937 A NO975937 A NO 975937A NO 319296 B1 NO319296 B1 NO 319296B1
- Authority
- NO
- Norway
- Prior art keywords
- particles
- analyte
- reaction
- matrix
- synthetic
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims description 65
- 239000003153 chemical reaction reagent Substances 0.000 title claims description 34
- 239000012491 analyte Substances 0.000 title claims description 27
- 239000007788 liquid Substances 0.000 title claims description 14
- 239000002245 particle Substances 0.000 claims description 137
- 238000006243 chemical reaction Methods 0.000 claims description 53
- 238000005119 centrifugation Methods 0.000 claims description 42
- 239000011159 matrix material Substances 0.000 claims description 42
- 230000004520 agglutination Effects 0.000 claims description 40
- 239000000427 antigen Substances 0.000 claims description 26
- 102000036639 antigens Human genes 0.000 claims description 25
- 108091007433 antigens Proteins 0.000 claims description 25
- 239000011541 reaction mixture Substances 0.000 claims description 22
- 210000003743 erythrocyte Anatomy 0.000 claims description 21
- 239000003446 ligand Substances 0.000 claims description 14
- 238000001514 detection method Methods 0.000 claims description 13
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 13
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 claims description 10
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 8
- 230000003993 interaction Effects 0.000 claims description 7
- 102000004169 proteins and genes Human genes 0.000 claims description 7
- 108090000623 proteins and genes Proteins 0.000 claims description 7
- 239000003599 detergent Substances 0.000 claims description 6
- 230000005484 gravity Effects 0.000 claims description 5
- 108020004707 nucleic acids Proteins 0.000 claims description 5
- 102000039446 nucleic acids Human genes 0.000 claims description 5
- 150000007523 nucleic acids Chemical class 0.000 claims description 5
- 102000015728 Mucins Human genes 0.000 claims description 4
- 108010063954 Mucins Proteins 0.000 claims description 4
- 230000000274 adsorptive effect Effects 0.000 claims description 4
- 210000000170 cell membrane Anatomy 0.000 claims description 4
- 239000003638 chemical reducing agent Substances 0.000 claims description 4
- 229920001577 copolymer Polymers 0.000 claims description 4
- 150000001720 carbohydrates Chemical class 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 239000012634 fragment Substances 0.000 claims description 3
- 239000005556 hormone Substances 0.000 claims description 3
- 229940088597 hormone Drugs 0.000 claims description 3
- 239000002207 metabolite Substances 0.000 claims description 3
- 229940051875 mucins Drugs 0.000 claims description 3
- 244000052769 pathogen Species 0.000 claims description 3
- 238000011895 specific detection Methods 0.000 claims description 3
- 210000004027 cell Anatomy 0.000 claims description 2
- 230000008105 immune reaction Effects 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 239000006166 lysate Substances 0.000 claims description 2
- 229920000620 organic polymer Polymers 0.000 claims description 2
- 150000003440 styrenes Chemical class 0.000 claims description 2
- 238000007899 nucleic acid hybridization Methods 0.000 claims 1
- 239000000725 suspension Substances 0.000 description 38
- 238000012360 testing method Methods 0.000 description 35
- 239000000243 solution Substances 0.000 description 30
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 27
- YMWUJEATGCHHMB-UHFFFAOYSA-N Dichloromethane Chemical compound ClCCl YMWUJEATGCHHMB-UHFFFAOYSA-N 0.000 description 18
- 239000000523 sample Substances 0.000 description 14
- 230000008878 coupling Effects 0.000 description 10
- 238000010168 coupling process Methods 0.000 description 10
- 238000005859 coupling reaction Methods 0.000 description 10
- 210000002966 serum Anatomy 0.000 description 9
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 238000004519 manufacturing process Methods 0.000 description 7
- 238000004062 sedimentation Methods 0.000 description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 6
- 239000000839 emulsion Substances 0.000 description 6
- 229920000642 polymer Polymers 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 239000013049 sediment Substances 0.000 description 6
- 230000006287 biotinylation Effects 0.000 description 5
- 238000007413 biotinylation Methods 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 108020004414 DNA Proteins 0.000 description 4
- 208000031886 HIV Infections Diseases 0.000 description 4
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 4
- 241000713340 Human immunodeficiency virus 2 Species 0.000 description 4
- 239000004372 Polyvinyl alcohol Substances 0.000 description 4
- YMOONIIMQBGTDU-VOTSOKGWSA-N [(e)-2-bromoethenyl]benzene Chemical compound Br\C=C\C1=CC=CC=C1 YMOONIIMQBGTDU-VOTSOKGWSA-N 0.000 description 4
- 238000011033 desalting Methods 0.000 description 4
- 239000000975 dye Substances 0.000 description 4
- 238000003018 immunoassay Methods 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 210000002381 plasma Anatomy 0.000 description 4
- 229920002451 polyvinyl alcohol Polymers 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 102000006395 Globulins Human genes 0.000 description 3
- 108010044091 Globulins Proteins 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 241000725303 Human immunodeficiency virus Species 0.000 description 3
- 108010090804 Streptavidin Proteins 0.000 description 3
- 101800000385 Transmembrane protein Proteins 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 239000008351 acetate buffer Substances 0.000 description 3
- 230000004523 agglutinating effect Effects 0.000 description 3
- 229960002685 biotin Drugs 0.000 description 3
- 239000011616 biotin Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 238000004040 coloring Methods 0.000 description 3
- 230000004069 differentiation Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000012674 dispersion polymerization Methods 0.000 description 3
- 239000012528 membrane Substances 0.000 description 3
- 229910052757 nitrogen Inorganic materials 0.000 description 3
- 239000013610 patient sample Substances 0.000 description 3
- 230000000704 physical effect Effects 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 238000010561 standard procedure Methods 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- OZAIFHULBGXAKX-UHFFFAOYSA-N 2-(2-cyanopropan-2-yldiazenyl)-2-methylpropanenitrile Chemical compound N#CC(C)(C)N=NC(C)(C)C#N OZAIFHULBGXAKX-UHFFFAOYSA-N 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 208000035473 Communicable disease Diseases 0.000 description 2
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 206010029719 Nonspecific reaction Diseases 0.000 description 2
- 108010039918 Polylysine Proteins 0.000 description 2
- 229920001213 Polysorbate 20 Polymers 0.000 description 2
- 239000004793 Polystyrene Substances 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- 101800001690 Transmembrane protein gp41 Proteins 0.000 description 2
- 239000007983 Tris buffer Substances 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 210000001124 body fluid Anatomy 0.000 description 2
- 239000010839 body fluid Substances 0.000 description 2
- 239000007795 chemical reaction product Substances 0.000 description 2
- 239000003086 colorant Substances 0.000 description 2
- 238000004132 cross linking Methods 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000007720 emulsion polymerization reaction Methods 0.000 description 2
- 238000001704 evaporation Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000035931 haemagglutination Effects 0.000 description 2
- 208000002672 hepatitis B Diseases 0.000 description 2
- BXWNKGSJHAJOGX-UHFFFAOYSA-N hexadecan-1-ol Chemical compound CCCCCCCCCCCCCCCCO BXWNKGSJHAJOGX-UHFFFAOYSA-N 0.000 description 2
- 239000004816 latex Substances 0.000 description 2
- 229920000126 latex Polymers 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 229920000656 polylysine Polymers 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 2
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 2
- 229920002223 polystyrene Polymers 0.000 description 2
- 239000004810 polytetrafluoroethylene Substances 0.000 description 2
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 description 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- BEOOHQFXGBMRKU-UHFFFAOYSA-N sodium cyanoborohydride Chemical compound [Na+].[B-]C#N BEOOHQFXGBMRKU-UHFFFAOYSA-N 0.000 description 2
- JQWHASGSAFIOCM-UHFFFAOYSA-M sodium periodate Chemical compound [Na+].[O-]I(=O)(=O)=O JQWHASGSAFIOCM-UHFFFAOYSA-M 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 230000000007 visual effect Effects 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 2
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 1
- CHRJZRDFSQHIFI-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;styrene Chemical compound C=CC1=CC=CC=C1.C=CC1=CC=CC=C1C=C CHRJZRDFSQHIFI-UHFFFAOYSA-N 0.000 description 1
- WGGLDBIZIQMEGH-UHFFFAOYSA-N 1-bromo-4-ethenylbenzene Chemical compound BrC1=CC=C(C=C)C=C1 WGGLDBIZIQMEGH-UHFFFAOYSA-N 0.000 description 1
- YMOONIIMQBGTDU-UHFFFAOYSA-N 2-bromoethenylbenzene Chemical class BrC=CC1=CC=CC=C1 YMOONIIMQBGTDU-UHFFFAOYSA-N 0.000 description 1
- JHWIEAWILPSRMU-UHFFFAOYSA-N 2-methyl-3-pyrimidin-4-ylpropanoic acid Chemical compound OC(=O)C(C)CC1=CC=NC=N1 JHWIEAWILPSRMU-UHFFFAOYSA-N 0.000 description 1
- IJJWOSAXNHWBPR-HUBLWGQQSA-N 5-[(3as,4s,6ar)-2-oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl]-n-(6-hydrazinyl-6-oxohexyl)pentanamide Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)NCCCCCC(=O)NN)SC[C@@H]21 IJJWOSAXNHWBPR-HUBLWGQQSA-N 0.000 description 1
- 108010088751 Albumins Proteins 0.000 description 1
- 102000009027 Albumins Human genes 0.000 description 1
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 108090001008 Avidin Proteins 0.000 description 1
- 239000004342 Benzoyl peroxide Substances 0.000 description 1
- OMPJBNCRMGITSC-UHFFFAOYSA-N Benzoylperoxide Chemical compound C=1C=CC=CC=1C(=O)OOC(=O)C1=CC=CC=C1 OMPJBNCRMGITSC-UHFFFAOYSA-N 0.000 description 1
- 102000004506 Blood Proteins Human genes 0.000 description 1
- 108010017384 Blood Proteins Proteins 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 241000759568 Corixa Species 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 1
- 108090000288 Glycoproteins Proteins 0.000 description 1
- 102000003886 Glycoproteins Human genes 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 102000004856 Lectins Human genes 0.000 description 1
- 108090001090 Lectins Proteins 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 240000002426 Persea americana var. drymifolia Species 0.000 description 1
- 239000012506 Sephacryl® Substances 0.000 description 1
- 229920005654 Sephadex Polymers 0.000 description 1
- 239000012507 Sephadex™ Substances 0.000 description 1
- 229920002684 Sepharose Polymers 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 206010047505 Visceral leishmaniasis Diseases 0.000 description 1
- 239000013566 allergen Substances 0.000 description 1
- 239000012062 aqueous buffer Substances 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 235000019400 benzoyl peroxide Nutrition 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 108091006004 biotinylated proteins Proteins 0.000 description 1
- 210000001772 blood platelet Anatomy 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229960000541 cetyl alcohol Drugs 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000011210 chromatographic step Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000007334 copolymerization reaction Methods 0.000 description 1
- 229920006037 cross link polymer Polymers 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000003748 differential diagnosis Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 229910001873 dinitrogen Inorganic materials 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 125000003700 epoxy group Chemical group 0.000 description 1
- 238000007306 functionalization reaction Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 229940072221 immunoglobulins Drugs 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 239000010781 infectious medical waste Substances 0.000 description 1
- 239000002523 lectin Substances 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000004811 liquid chromatography Methods 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 238000005497 microtitration Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- -1 mono- Chemical class 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 150000002482 oligosaccharides Polymers 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920000729 poly(L-lysine) polymer Polymers 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 229920000193 polymethacrylate Polymers 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000001267 polyvinylpyrrolidone Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000004007 reversed phase HPLC Methods 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- 239000007974 sodium acetate buffer Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- JVBXVOWTABLYPX-UHFFFAOYSA-L sodium dithionite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])=O JVBXVOWTABLYPX-UHFFFAOYSA-L 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 239000003774 sulfhydryl reagent Substances 0.000 description 1
- 238000010557 suspension polymerization reaction Methods 0.000 description 1
- 230000008961 swelling Effects 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- TUQOTMZNTHZOKS-UHFFFAOYSA-N tributylphosphine Chemical compound CCCCP(CCCC)CCCC TUQOTMZNTHZOKS-UHFFFAOYSA-N 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/823—Immunogenic carrier or carrier per se
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
- Y10S530/815—Carrier is a synthetic polymer
Landscapes
- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP96120421A EP0849595B1 (de) | 1996-12-18 | 1996-12-18 | Synthetische Partikel als Agglutinationsreagenzien |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| NO975937D0 NO975937D0 (no) | 1997-12-17 |
| NO975937L NO975937L (no) | 1998-06-19 |
| NO319296B1 true NO319296B1 (no) | 2005-07-11 |
Family
ID=8223513
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| NO19975937A NO319296B1 (no) | 1996-12-18 | 1997-12-17 | Fremgangsmate og reagens-kit for pavisning av en analytt i en provevaeske. |
Country Status (15)
| Country | Link |
|---|---|
| US (1) | US6203706B1 (xx) |
| EP (1) | EP0849595B1 (xx) |
| JP (1) | JPH10197534A (xx) |
| CN (1) | CN1123772C (xx) |
| AR (1) | AR010364A1 (xx) |
| AT (1) | ATE201100T1 (xx) |
| AU (1) | AU724475B2 (xx) |
| BR (1) | BR9705648A (xx) |
| DE (1) | DE59606885D1 (xx) |
| DK (1) | DK0849595T3 (xx) |
| ES (1) | ES2156610T3 (xx) |
| GR (1) | GR3035882T3 (xx) |
| HK (1) | HK1008244A1 (xx) |
| NO (1) | NO319296B1 (xx) |
| PT (1) | PT849595E (xx) |
Families Citing this family (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE69938973D1 (de) * | 1998-09-10 | 2008-08-07 | Fujifilm Corp | Verfahren zur feststellung von einer thiol enthaltenden zusammensetzung |
| DE19857692C1 (de) * | 1998-12-14 | 2000-08-24 | Evotec Biosystems Ag | Verfahren und Vorrichtung zur zellspurbasierten Zelluntersuchung |
| DE10122806A1 (de) * | 2001-05-10 | 2002-11-14 | Holger Kiesewetter | Verfahren zum Nachweis von Blutzell-Antigenen und gegen diese gerichtete Antikörper |
| JP2003107091A (ja) * | 2001-09-28 | 2003-04-09 | Olympus Optical Co Ltd | 生物学的検査のための分離体および検査方法 |
| US6890765B2 (en) * | 2001-11-02 | 2005-05-10 | Roche Diagnostics Operations, Inc. | Particles for immunoassays and methods for treating the same |
| US6777246B2 (en) | 2001-12-18 | 2004-08-17 | Roche Diagnostics Gmbh | Tertiary amine compounds for use in immunoassays |
| DE10239568A1 (de) * | 2002-08-23 | 2004-03-04 | Deutsches Rotes Kreuz Blutspendedienst Baden-Württemberg, Gemeinnützige GmbH | Verfahren zum Nachweis von Antikörpern und/oder Antigenen in einer Testflüssigkeit sowie zur Blutgruppenbestimmung |
| AU2004200467A1 (en) * | 2003-02-10 | 2004-08-26 | Ortho-Clinical Diagnostics, Inc. | Antibodies for discrimination of prions |
| US20070054405A1 (en) * | 2003-10-23 | 2007-03-08 | Ortho-Clinical Diagnostics, Inc. | Patient sample classification based upon low angle light scattering |
| DE102004005193B4 (de) * | 2004-02-02 | 2006-08-24 | Medion Diagnostics Gmbh | Vorrichtung zur Separation einzelner Partikel von Partikel-Agglutinationen |
| WO2005111572A1 (ja) * | 2004-05-18 | 2005-11-24 | Eisai R & D Management Co., Ltd. | 試料調製装置及び方法 |
| EP1969011B1 (en) | 2006-01-03 | 2012-05-16 | TTO Nord AS | Preparation for treatment of neonatal alloimmune thrombocytopenia (nait) |
| GB0706558D0 (en) * | 2007-04-03 | 2007-05-09 | Common Services Agency For The | Diagnostic assay |
| US8058073B2 (en) | 2008-01-30 | 2011-11-15 | Ortho-Clinical Diagnostics, Inc. | Immunodiagnostic test cards having indicating indicia |
| US8076126B2 (en) | 2008-07-18 | 2011-12-13 | Ortho-Clinical Diagnostics, Inc. | Single column immunological test elements |
| WO2010025756A1 (en) * | 2008-09-03 | 2010-03-11 | Stichting Sanquin Bloedvoorziening | Reaction vessel capable of detecting carrier-bound analyte complexes, method for detecting carrier-bound analyte complexes, and kit of parts |
| CN101957366A (zh) * | 2009-07-15 | 2011-01-26 | 英科新创(厦门)科技有限公司 | 人红细胞膜抗原包被的微球及其应用 |
| JP5452217B2 (ja) * | 2009-12-28 | 2014-03-26 | シスメックス株式会社 | 試料中のｃ型肝炎ウイルスの有無を判定する方法、及びｃ型肝炎ウイルスの有無を判定するための試薬キット |
| DE102012201717A1 (de) | 2012-02-06 | 2013-08-08 | AusBio Laboratories Co, Ltd. | Probenträger-Zentrifuge |
| US9354242B2 (en) * | 2012-10-16 | 2016-05-31 | Ortho-Clinical Diagnostics, Inc. | Glass bead flow rates to facilitate immunodiagnostic test element manufacture |
| WO2023282862A1 (en) * | 2021-07-05 | 2023-01-12 | Sakarya Universitesi Rektorlugu | A test method for diagnosis of covid-19 disease |
Family Cites Families (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2322562C2 (de) * | 1972-11-06 | 1984-03-29 | Pharmacia AB, Uppsala | Verfahren zur Bestimmung von Antigenen in einer Probe |
| US4021534A (en) * | 1975-12-12 | 1977-05-03 | Hoffmann-La Roche Inc. | Radioimmunoassay |
| GB2013211B (en) * | 1978-01-26 | 1982-06-30 | Technicon Instr | Immunoassays using f(ab')2 fragments |
| JPS566161A (en) * | 1979-06-28 | 1981-01-22 | Seikagaku Kogyo Co Ltd | Erythropoietin sensitized latex |
| JPS58209984A (ja) * | 1982-05-28 | 1983-12-07 | Japan Synthetic Rubber Co Ltd | 粒子状重合体よりなる担体 |
| US4605686A (en) * | 1984-03-13 | 1986-08-12 | Sekisui Kagaku Kogyo Kabushiki Kaisha | Latex for immunoserological tests and a method for the production of the same |
| US4659658A (en) * | 1984-12-26 | 1987-04-21 | Becton, Dickinson And Company | Lectin-coated latex agglutination assay for Neisseria gonorrhoeae |
| FR2577321B1 (fr) | 1985-02-08 | 1989-04-28 | Lapierre Yves | Dispositif et procede de mise en evidence d'agglutinats erythrocytaires |
| US4891324A (en) * | 1987-01-07 | 1990-01-02 | Syntex (U.S.A.) Inc. | Particle with luminescer for assays |
| EP0279525A1 (en) * | 1987-01-23 | 1988-08-24 | Seitetsu Kagaku Co., Ltd. | Blood group substance-carrying latex and process for preparing the same |
| US5338689A (en) * | 1987-08-24 | 1994-08-16 | Stiftung Fur Diagnostische Forschung | Method and card for detecting antigens and/or antibodies |
| JPH087215B2 (ja) | 1987-08-24 | 1996-01-29 | シュティフツング・フュア・ディアグノスティッシュ・フォルシュンク | 抗原および／又は抗体の検出方法および検出用の試験キット |
| US4829011A (en) * | 1987-08-27 | 1989-05-09 | Biotrack, Inc. | Agglutination assay |
| EP0348174A3 (en) * | 1988-06-23 | 1991-05-22 | Bio-Rad Laboratories, Inc. | Sperm antibody test |
| FR2638848B1 (fr) * | 1988-11-04 | 1993-01-22 | Chemunex Sa | Procede de detection et/ou de dosage dans un milieu liquide ou semi-liquide d'au moins une substance organique, biologique ou medicamenteuse soluble, par une methode d'agglutination |
| CA2055095C (en) | 1990-11-09 | 2003-05-13 | Johnna B. Hawk | Column agglutination assay and device |
| EP0557546B1 (de) | 1992-02-25 | 1997-05-07 | Stiftung Für Diagnostische Forschung | Kopplung von Antigenen und Antikörpern an nicht-fixierte Erythrozyten |
| US5552064A (en) * | 1993-02-26 | 1996-09-03 | Ortho Diagnostic Systems, Inc. | Column agglutination assay and device using biphasic centrifugation |
| US5491067A (en) | 1993-07-15 | 1996-02-13 | Ortho Diagnostic Systems Inc. | Agglutination reaction and separation vessel |
| US5665558A (en) * | 1994-05-17 | 1997-09-09 | Gamma Biologicals, Inc. | Method and apparatus useful for detecting bloodgroup antigens and antibodies |
-
1996
- 1996-12-18 EP EP96120421A patent/EP0849595B1/de not_active Expired - Lifetime
- 1996-12-18 ES ES96120421T patent/ES2156610T3/es not_active Expired - Lifetime
- 1996-12-18 AT AT96120421T patent/ATE201100T1/de active
- 1996-12-18 PT PT96120421T patent/PT849595E/pt unknown
- 1996-12-18 DE DE59606885T patent/DE59606885D1/de not_active Expired - Lifetime
- 1996-12-18 DK DK96120421T patent/DK0849595T3/da active
-
1997
- 1997-12-05 AU AU46907/97A patent/AU724475B2/en not_active Ceased
- 1997-12-16 BR BR9705648A patent/BR9705648A/pt unknown
- 1997-12-16 US US08/991,883 patent/US6203706B1/en not_active Expired - Lifetime
- 1997-12-16 JP JP9346351A patent/JPH10197534A/ja active Pending
- 1997-12-17 CN CN97125532A patent/CN1123772C/zh not_active Expired - Fee Related
- 1997-12-17 NO NO19975937A patent/NO319296B1/no not_active IP Right Cessation
- 1997-12-18 AR ARP970105972A patent/AR010364A1/es active IP Right Grant
-
1998
- 1998-07-21 HK HK98109323A patent/HK1008244A1/xx not_active IP Right Cessation
-
2001
- 2001-05-16 GR GR20010400735T patent/GR3035882T3/el unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JPH10197534A (ja) | 1998-07-31 |
| US6203706B1 (en) | 2001-03-20 |
| CN1123772C (zh) | 2003-10-08 |
| ATE201100T1 (de) | 2001-05-15 |
| AU724475B2 (en) | 2000-09-21 |
| AU4690797A (en) | 1998-06-25 |
| NO975937D0 (no) | 1997-12-17 |
| EP0849595B1 (de) | 2001-05-09 |
| PT849595E (pt) | 2001-10-31 |
| NO975937L (no) | 1998-06-19 |
| HK1008244A1 (en) | 1999-05-07 |
| DK0849595T3 (da) | 2001-05-28 |
| CN1191312A (zh) | 1998-08-26 |
| BR9705648A (pt) | 1999-02-23 |
| EP0849595A1 (de) | 1998-06-24 |
| DE59606885D1 (de) | 2001-06-13 |
| ES2156610T3 (es) | 2001-07-01 |
| GR3035882T3 (en) | 2001-08-31 |
| AR010364A1 (es) | 2000-06-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| NO319296B1 (no) | Fremgangsmate og reagens-kit for pavisning av en analytt i en provevaeske. | |
| EP0462644B1 (en) | Biologically active reagents prepared from carboxy-containing polymer, analytical element and methods of use | |
| US4656144A (en) | Immunoparticles and process for preparing same | |
| EP0038960B1 (en) | Diagnostic reagents for immunological tests and a process for preparing the same | |
| NO170825B (no) | Fastfasesystem og apparat for detektering av en eller flere analytter i en vaeskeproeve, samt anvendelse av apparatetfor immunoanalyse | |
| US20050221297A1 (en) | Diagnostic reagent for hepatitis C virus infection | |
| MXPA05005951A (es) | Reduccion de efecto de gancho en dispositivos de ensayo a base de membrana. | |
| EP0468585A2 (en) | Biologically active reagent, analytical element and methods for use of the reagent | |
| JP3840521B2 (ja) | ウイルス検出方法およびウイルス検査用キット | |
| JPH05310849A (ja) | 多官能性親水性球状微粒子、その製造方法及びその使用方法 | |
| WO2018043584A1 (ja) | 異なる方式で抗原を固定化した抗原担持不溶性担体粒子を用いる抗体測定法、抗体測定用試薬 | |
| JP2001021563A (ja) | 特異的結合体 | |
| US4792527A (en) | Method of assaying biologically active substances and labelling agents therefor | |
| CN113125715B (zh) | 一种人类免疫缺陷病毒抗体检测试剂盒及其应用 | |
| US6548310B1 (en) | Particle for diagnostic agent and turbidmetric immunoassay using the same | |
| JP4005093B2 (ja) | Ｃ型肝炎ウイルス感染症診断薬および抗ｈｃｖ抗体検出方法 | |
| JP2004117068A (ja) | 免疫測定試薬および免疫測定方法 | |
| JPS6315552B2 (xx) | ||
| JP2005274584A (ja) | Ｃ型肝炎ウイルス感染症診断薬の製造方法 | |
| JPH09152432A (ja) | 低分子抗原結合高分子物質による抗体の測定方法及び測定試薬 | |
| JP2001091515A (ja) | 標識特異的結合体 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MM1K | Lapsed by not paying the annual fees |