ES2101763T3 - Acoplamiento de antigenos y anticuerpos a eritrocitos no fijados. - Google Patents

Acoplamiento de antigenos y anticuerpos a eritrocitos no fijados.

Info

Publication number
ES2101763T3
ES2101763T3 ES92103185T ES92103185T ES2101763T3 ES 2101763 T3 ES2101763 T3 ES 2101763T3 ES 92103185 T ES92103185 T ES 92103185T ES 92103185 T ES92103185 T ES 92103185T ES 2101763 T3 ES2101763 T3 ES 2101763T3
Authority
ES
Spain
Prior art keywords
erythrocytes
analyte
antibodies
binding partner
incubate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES92103185T
Other languages
English (en)
Inventor
Ives Lapierre
Dieter Josef
Jean-R Adam
Susanne Greber-Widmer
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Stiftung fur Diagnostische Forschung
Original Assignee
Stiftung fur Diagnostische Forschung
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Stiftung fur Diagnostische Forschung filed Critical Stiftung fur Diagnostische Forschung
Application granted granted Critical
Publication of ES2101763T3 publication Critical patent/ES2101763T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • G01N33/555—Red blood cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
    • G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Urology & Nephrology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Endocrinology (AREA)
  • Mycology (AREA)
  • Reproductive Health (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

Abstract

PARA LA DETERMINACION DE UNA MUESTRA DE ANALISIS EN UNA SOLUCION DE PRUEBA ACUOSA POR MEDIO DE AGLUTINACION DE ERITROCITOS NO FIJADOS SE DISPONE SEGUN LA INVENCION, QUE (A) LOS ERITROCITOS SE INCUBAN CON UNO O MAS ANTICUERPOS Y/O DERIVADOS DE ANTICUERPOS QUE CONDUCEN A UNA AGLUTINACION ANTICIPADA, DONDE LOS ANTICUERPOS Y/O DERIVADOS DE ANTICUERPOS SON DIRIGIDOS CONTRA UNO O MAS ANTIGENOS SOBRE LA MEMBRANA DEL ERITROCITO Y DONDE LOS ANTICUERPOS Y/O DERIVADOS DE ANTICUERPOS ANTES O DESPUES DE LA INCUBACION SON ACOPLADOS CON LOS ERITROCITOS EN UN PRIMER ACOMPAÑANTE DE ENLACE DE UN PAR DE ENLACES DE ALTA AFINIDAD, (B) UN SEGUNDO ACOMPAÑANTE DE ENLACE DEL PAR DE ENLACE DE ALTA AFINIDAD SE INCUBA CON LOS ERITROCITOS MANEJADOS EN EL APARTADO (A), CON LO QUE EL SEGUNDO PARTNER DE ENLACE ES ELEGIDO DE TAL MODO, QUE MUESTRA POR MOLECULA MAS DE UN LUGAR DE ENLACE PARA EL PRIMER PARTNER DE ENLACE, (C) EL PRIMER PARTNER DE ENLACE DEL PAR DE ENLACE DE ALTA AFINIDAD, QUE ESTA ACOPLADO CON LA MUESTRA DE ANALISIS Y/O DE UN DETERMINANTE ACTIVO ANTIGENO DE LA MUESTRA DE ANALISIS, SE INCUBA CON LOS ERITROCITOS MANEJADOS EN EL APARTADO (B), (D) UNA SOLUCION DE PRUEBA, QUE HA SIDO MANEJADA CON UN ANTICUERPO O DERIVADO DE ANTICUERPO DIRIGIDO CONTRA LA MUESTRA DE ANALISIS, SE INCUBA CON ERITROCITOS MANEJADOS EN EL APARTADO (C) Y (E) A CONTINUACION DE LA APARICION O AUSENCIA DE UNA REACCION DE AGLUTINACION, SE DETERMINE LA FALTA O EXISTENCIA DE LA NUESTRA DE ANALISIS EN LA SOLUCION DE PRUEBA.
ES92103185T 1992-02-25 1992-02-25 Acoplamiento de antigenos y anticuerpos a eritrocitos no fijados. Expired - Lifetime ES2101763T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP92103185A EP0557546B1 (de) 1992-02-25 1992-02-25 Kopplung von Antigenen und Antikörpern an nicht-fixierte Erythrozyten

Publications (1)

Publication Number Publication Date
ES2101763T3 true ES2101763T3 (es) 1997-07-16

Family

ID=8209361

Family Applications (1)

Application Number Title Priority Date Filing Date
ES92103185T Expired - Lifetime ES2101763T3 (es) 1992-02-25 1992-02-25 Acoplamiento de antigenos y anticuerpos a eritrocitos no fijados.

Country Status (9)

Country Link
EP (1) EP0557546B1 (es)
JP (1) JPH0688822A (es)
AT (1) ATE152835T1 (es)
CA (1) CA2090392C (es)
DE (1) DE59208460D1 (es)
DK (1) DK0557546T3 (es)
ES (1) ES2101763T3 (es)
GR (1) GR3023970T3 (es)
HK (1) HK1002126A1 (es)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5665558A (en) * 1994-05-17 1997-09-09 Gamma Biologicals, Inc. Method and apparatus useful for detecting bloodgroup antigens and antibodies
US5905028A (en) * 1994-05-17 1999-05-18 Gamma Biologicals, Inc. Method and apparatus useful for detecting bloodgroup antigens and antibodies
DE59606885D1 (de) 1996-12-18 2001-06-13 Diagnostische Forsch Stiftung Synthetische Partikel als Agglutinationsreagenzien
US7939283B2 (en) 2001-11-01 2011-05-10 Fisher Scientific Company L.L.C. Analyte binding turbidity assay
CN109991405B (zh) * 2017-12-29 2023-01-24 上海索昕生物科技有限公司 一种免疫检测试剂盒及其应用
CN112114157B (zh) * 2020-09-22 2024-03-15 天津大学 基于细胞代替荧光编码微球检测hcg的方法

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BE627961A (es) * 1962-02-05
FR2486657A1 (fr) * 1980-07-09 1982-01-15 Pasteur Institut Procede de detection et de dosage d'une substance biologique par erythroadsorption
US4433059A (en) * 1981-09-08 1984-02-21 Ortho Diagnostic Systems Inc. Double antibody conjugate
FR2577321B1 (fr) * 1985-02-08 1989-04-28 Lapierre Yves Dispositif et procede de mise en evidence d'agglutinats erythrocytaires
JPH0825888B2 (ja) * 1986-01-30 1996-03-13 フレッド ハッチンソン キャンサ− リサ−チ センタ− 免疫選択法
DE3705686C2 (de) * 1987-02-23 1995-11-30 Boehringer Mannheim Gmbh Verfahren zur Bestimmung von Antikörpern
JPH087215B2 (ja) * 1987-08-24 1996-01-29 シュティフツング・フュア・ディアグノスティッシュ・フォルシュンク 抗原および／又は抗体の検出方法および検出用の試験キット

Also Published As

Publication number Publication date
HK1002126A1 (en) 1998-07-31
CA2090392A1 (en) 1993-08-26
EP0557546B1 (de) 1997-05-07
GR3023970T3 (en) 1997-10-31
ATE152835T1 (de) 1997-05-15
DE59208460D1 (de) 1997-06-12
JPH0688822A (ja) 1994-03-29
EP0557546A1 (de) 1993-09-01
CA2090392C (en) 2000-01-11
DK0557546T3 (da) 1997-10-20

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