CA2090392A1 - Coupling of antigens and antibodies to non-fixed erythrocytes - Google Patents
Coupling of antigens and antibodies to non-fixed erythrocytesInfo
- Publication number
- CA2090392A1 CA2090392A1 CA2090392A CA2090392A CA2090392A1 CA 2090392 A1 CA2090392 A1 CA 2090392A1 CA 2090392 A CA2090392 A CA 2090392A CA 2090392 A CA2090392 A CA 2090392A CA 2090392 A1 CA2090392 A1 CA 2090392A1
- Authority
- CA
- Canada
- Prior art keywords
- erythrocytes
- analyte
- antibodies
- binding partner
- incubate
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000003743 erythrocyte Anatomy 0.000 title abstract 7
- 239000000427 antigen Substances 0.000 title abstract 2
- 102000036639 antigens Human genes 0.000 title abstract 2
- 108091007433 antigens Proteins 0.000 title abstract 2
- 230000008878 coupling Effects 0.000 title 1
- 238000010168 coupling process Methods 0.000 title 1
- 238000005859 coupling reaction Methods 0.000 title 1
- 239000012491 analyte Substances 0.000 abstract 5
- 230000004520 agglutination Effects 0.000 abstract 3
- 239000011230 binding agent Substances 0.000 abstract 3
- 239000012488 sample solution Substances 0.000 abstract 3
- 210000003617 erythrocyte membrane Anatomy 0.000 abstract 1
- 238000011534 incubation Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000002028 premature Effects 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
- G01N33/555—Red blood cell
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Immunology (AREA)
- Biomedical Technology (AREA)
- Urology & Nephrology (AREA)
- Chemical & Material Sciences (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Pathology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Endocrinology (AREA)
- Mycology (AREA)
- Reproductive Health (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
To determine an analyte in an aqueous sample solution by agglutination of non-fixed erythrocytes, the procedure according to the invention is (a) to incubate erythrocytes with one or more antibodies and/or antibody derivatives which do not lead to premature agglutination, the antibodies and/or antibody derivatives being directed against one or more antigens on the erythrocyte membrane, and the antibodies and/or antibody derivatives being coupled, before or after the incubation with the erythrocytes, to a first binding partner in a pair of high-affinity binders, (b) to incubate a second binding partner of the pair of high-affinity binders with the treated erythrocytes from (a), the second binding partner being chosen so that it has, per molecule, more than one binding site for the first binding partner, (c) to incubate the first binding partner, which is coupled to the analyte and/or to an antigenically active determinant of the analyte, of the pair of high-affinity binders with the treated erythrocytes from (b), (d) to incubate a sample solution, which has been treated with an antibody or antibody derivative directed against the analyte, with the treated erythrocytes from (c), and (e) to determine, on the basis of the occurrence or non-occurrence of an agglutination reaction, the absence or the presence, respectively, of the analyte in the sample solution. <IMAGE>
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP92103185A EP0557546B1 (en) | 1992-02-25 | 1992-02-25 | Binding of antigens and antibodies to non-fixed erythrocytes |
| EPEP92103185.2 | 1992-02-25 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2090392A1 true CA2090392A1 (en) | 1993-08-26 |
| CA2090392C CA2090392C (en) | 2000-01-11 |
Family
ID=8209361
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002090392A Expired - Lifetime CA2090392C (en) | 1992-02-25 | 1993-02-25 | Coupling of antigens and antibodies to non-fixed erythrocytes |
Country Status (9)
| Country | Link |
|---|---|
| EP (1) | EP0557546B1 (en) |
| JP (1) | JPH0688822A (en) |
| AT (1) | ATE152835T1 (en) |
| CA (1) | CA2090392C (en) |
| DE (1) | DE59208460D1 (en) |
| DK (1) | DK0557546T3 (en) |
| ES (1) | ES2101763T3 (en) |
| GR (1) | GR3023970T3 (en) |
| HK (1) | HK1002126A1 (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5665558A (en) * | 1994-05-17 | 1997-09-09 | Gamma Biologicals, Inc. | Method and apparatus useful for detecting bloodgroup antigens and antibodies |
| US5905028A (en) * | 1994-05-17 | 1999-05-18 | Gamma Biologicals, Inc. | Method and apparatus useful for detecting bloodgroup antigens and antibodies |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE59606885D1 (en) | 1996-12-18 | 2001-06-13 | Diagnostische Forsch Stiftung | Synthetic particles as agglutination reagents |
| US7939283B2 (en) * | 2001-11-01 | 2011-05-10 | Fisher Scientific Company L.L.C. | Analyte binding turbidity assay |
| CN109991405B (en) * | 2017-12-29 | 2023-01-24 | 上海索昕生物科技有限公司 | Immunoassay kit and application thereof |
| CN112114157B (en) * | 2020-09-22 | 2024-03-15 | 天津大学 | Method for detecting HCG (HCG) based on cell replacement fluorescent coding microsphere |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| BE633260A (en) * | 1962-02-05 | |||
| FR2486657A1 (en) * | 1980-07-09 | 1982-01-15 | Pasteur Institut | METHOD OF DETECTING AND ASSAYING A BIOLOGICAL SUBSTANCE BY ERYTHROADSORPTION |
| US4433059A (en) * | 1981-09-08 | 1984-02-21 | Ortho Diagnostic Systems Inc. | Double antibody conjugate |
| FR2577321B1 (en) * | 1985-02-08 | 1989-04-28 | Lapierre Yves | DEVICE AND METHOD FOR EVIDENCE OF ERYTHROCYTA AGGLUTINATES |
| EP0260280B2 (en) * | 1986-01-30 | 1995-09-20 | Fred Hutchinson Cancer Research Center | Immunoselection method |
| DE3705686C2 (en) * | 1987-02-23 | 1995-11-30 | Boehringer Mannheim Gmbh | Methods for the determination of antibodies |
| JPH087215B2 (en) * | 1987-08-24 | 1996-01-29 | シュティフツング・フュア・ディアグノスティッシュ・フォルシュンク | Method for detecting antigen and / or antibody and test kit for detection |
-
1992
- 1992-02-25 ES ES92103185T patent/ES2101763T3/en not_active Expired - Lifetime
- 1992-02-25 DK DK92103185.2T patent/DK0557546T3/en active
- 1992-02-25 EP EP92103185A patent/EP0557546B1/en not_active Expired - Lifetime
- 1992-02-25 AT AT92103185T patent/ATE152835T1/en active
- 1992-02-25 DE DE59208460T patent/DE59208460D1/en not_active Expired - Lifetime
-
1993
- 1993-02-25 CA CA002090392A patent/CA2090392C/en not_active Expired - Lifetime
- 1993-02-25 JP JP5036463A patent/JPH0688822A/en active Pending
-
1997
- 1997-07-01 GR GR970401621T patent/GR3023970T3/en unknown
-
1998
- 1998-02-16 HK HK98101191A patent/HK1002126A1/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5665558A (en) * | 1994-05-17 | 1997-09-09 | Gamma Biologicals, Inc. | Method and apparatus useful for detecting bloodgroup antigens and antibodies |
| US5905028A (en) * | 1994-05-17 | 1999-05-18 | Gamma Biologicals, Inc. | Method and apparatus useful for detecting bloodgroup antigens and antibodies |
Also Published As
| Publication number | Publication date |
|---|---|
| DE59208460D1 (en) | 1997-06-12 |
| HK1002126A1 (en) | 1998-07-31 |
| GR3023970T3 (en) | 1997-10-31 |
| ES2101763T3 (en) | 1997-07-16 |
| ATE152835T1 (en) | 1997-05-15 |
| JPH0688822A (en) | 1994-03-29 |
| EP0557546B1 (en) | 1997-05-07 |
| DK0557546T3 (en) | 1997-10-20 |
| EP0557546A1 (en) | 1993-09-01 |
| CA2090392C (en) | 2000-01-11 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |